##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003839_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 485584 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.002897541928895 31.0 31.0 34.0 30.0 34.0 2 32.153695344162905 33.0 31.0 34.0 30.0 34.0 3 32.24342441266599 34.0 31.0 34.0 30.0 34.0 4 35.835107828923526 37.0 35.0 37.0 35.0 37.0 5 35.76712371083067 37.0 35.0 37.0 35.0 37.0 6 35.90800561797753 37.0 35.0 37.0 35.0 37.0 7 35.89556698738014 37.0 35.0 37.0 35.0 37.0 8 35.912911051434975 37.0 35.0 37.0 35.0 37.0 9 37.547931974694386 39.0 37.0 39.0 35.0 39.0 10-11 37.45985349599657 39.0 37.0 39.0 34.5 39.0 12-13 37.45900091436291 39.0 37.0 39.0 34.0 39.0 14-15 38.51403464694059 40.0 38.0 41.0 34.0 41.0 16-17 38.62371083066988 40.0 38.0 41.0 34.5 41.0 18-19 38.70087461695608 40.0 38.0 41.0 34.0 41.0 20-21 38.650947106988696 40.0 38.0 41.0 34.0 41.0 22-23 38.57755609740025 40.0 38.0 41.0 34.0 41.0 24-25 38.38674256153415 40.0 38.0 41.0 34.0 41.0 26-27 38.072250115325055 40.0 37.5 41.0 33.5 41.0 28-29 37.95234810043165 40.0 37.0 41.0 33.0 41.0 30-31 37.87337309301789 40.0 37.0 41.0 33.0 41.0 32-33 37.583281162476524 40.0 37.0 41.0 33.0 41.0 34-35 37.457412105835445 39.0 36.5 41.0 33.0 41.0 36-37 37.24786957560381 39.5 36.5 41.0 32.0 41.0 38-39 37.030601296583086 39.0 36.0 40.5 31.0 41.0 40-41 37.212398884642 39.5 36.5 41.0 31.5 41.0 42-43 37.11198165507925 40.0 36.5 41.0 31.0 41.0 44-45 36.80730213516097 39.5 36.0 41.0 30.5 41.0 46-47 36.63008871791492 40.0 36.0 41.0 30.0 41.0 48-49 36.278241251771064 39.0 36.0 41.0 30.0 41.0 50-51 35.891318494843325 39.0 35.0 41.0 28.0 41.0 52-53 35.80965394246927 39.0 35.0 41.0 28.0 41.0 54-55 35.665932155919464 39.0 35.0 41.0 28.0 41.0 56-57 35.29386161817523 38.5 34.5 40.0 27.0 41.0 58-59 35.00147966160335 38.0 34.0 40.0 26.0 41.0 60-61 34.42459800981911 38.0 34.0 40.0 25.5 41.0 62-63 34.275517727107975 37.5 34.0 40.0 26.0 41.0 64-65 33.81432048831922 37.0 33.5 39.5 24.0 41.0 66-67 33.40266668588751 36.5 33.0 39.0 22.5 41.0 68-69 32.93023246235461 36.0 32.5 39.0 22.5 40.0 70-71 32.29264658637847 35.0 31.5 37.5 21.5 40.0 72-73 31.764533015914857 35.0 31.0 37.0 20.0 39.5 74-75 31.39057197106989 35.0 31.0 37.0 18.5 39.0 76-77 30.488282150976968 34.0 30.0 35.5 18.0 38.5 78-79 30.9128091123266 34.0 31.0 36.0 18.0 38.5 80-81 30.56381388184125 34.0 31.0 35.5 13.0 37.0 82-83 30.222008962404033 34.0 30.5 35.0 9.5 37.0 84-85 29.944855061122276 34.0 30.0 35.0 7.0 36.5 86-87 29.816868966028537 34.0 30.0 35.0 4.5 36.0 88-89 29.502226185376784 34.0 30.0 35.0 2.0 36.0 90-91 29.173739250057665 34.0 29.0 35.0 2.0 35.5 92-93 28.901118035190617 34.0 29.0 35.0 2.0 35.0 94-95 28.454641215526046 34.0 29.0 35.0 2.0 35.0 96-97 27.963204100629344 34.0 29.0 35.0 2.0 35.0 98-99 27.783810833964875 34.0 29.0 35.0 2.0 35.0 100-101 26.854537011104156 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.18358204905044317 1101 2 0.19316899660039866 1101 3 0.2528174427198948 1101 4 0.1696931997787985 1101 5 0.14656688768653936 1101 6 0.2266898171426277 1101 7 0.2753190027136725 1101 8 0.11619768511602757 1101 9 0.30654014427846477 1101 10-11 0.25572384097525 1101 12-13 0.23666576423050145 1101 14-15 0.3750207834796413 1101 16-17 0.2794252793960439 1101 18-19 0.23800796139914127 1101 20-21 0.2976887600559053 1101 22-23 0.2846156573113561 1101 24-25 0.31257975865991483 1101 26-27 0.4177667107413967 1101 28-29 0.3788289542933967 1101 30-31 0.30501780639011855 1101 32-33 0.37991631910535517 1101 34-35 0.15680667683371752 1101 36-37 0.40960102858733904 1101 38-39 0.3861352239546747 1101 40-41 0.30531018499382157 1101 42-43 0.5099651240861007 1101 44-45 0.5512818837174152 1101 46-47 0.32957783371008276 1101 48-49 0.29482997508990394 1101 50-51 0.11861966054083695 1101 52-53 0.14807308844811473 1101 54-55 0.27933227218311174 1101 56-57 0.376935265576428 1101 58-59 0.18148350757781628 1101 60-61 0.24913141404989148 1101 62-63 0.2987791594737885 1101 64-65 0.42235119010239686 1101 66-67 0.38364429739601036 1101 68-69 0.4679965822047123 1101 70-71 0.5628387124973386 1101 72-73 0.3239747144962557 1101 74-75 0.19712359287233738 1101 76-77 -0.0770200723475476 1101 78-79 0.23335596353790677 1101 80-81 -0.09154431639463212 1101 82-83 0.4294881268574855 1101 84-85 0.3183501191920044 1101 86-87 0.22588352505099962 1101 88-89 0.4250729403148519 1101 90-91 0.3926662072553597 1101 92-93 0.3635447223842263 1101 94-95 0.19341751999298396 1101 96-97 0.09586632632638015 1101 98-99 0.1261654947267239 1101 100-101 0.15567791178139245 1102 1 -0.05387667713008781 1102 2 0.06565685405065835 1102 3 -0.07344916174219662 1102 4 -0.07714322750875624 1102 5 -0.07519241677953659 1102 6 0.07673701305448333 1102 7 0.09030379514206999 1102 8 0.04369680810386711 1102 9 0.12965854634423835 1102 10-11 0.17642024287466285 1102 12-13 0.1522754956297021 1102 14-15 -0.08701272742604971 1102 16-17 0.05624286448431093 1102 18-19 0.005762484792761313 1102 20-21 0.015115443648006988 1102 22-23 -0.1431040749196555 1102 24-25 0.0789726528723591 1102 26-27 -0.07138727089378705 1102 28-29 -0.07804013039523738 1102 30-31 -0.08052035358413434 1102 32-33 -0.13604891866438606 1102 34-35 -0.07385836962080816 1102 36-37 -0.37510243280785716 1102 38-39 -0.13974296357764615 1102 40-41 0.026400428640464213 1102 42-43 0.13763277806955188 1102 44-45 0.38067137476104307 1102 46-47 -0.18559176416628276 1102 48-49 -0.33502930514530505 1102 50-51 -0.2689980127911227 1102 52-53 -0.21170622997782118 1102 54-55 -0.27911471107589847 1102 56-57 -0.0944867960712017 1102 58-59 -0.42076526008447956 1102 60-61 -0.27656413796145074 1102 62-63 -0.6964705087082095 1102 64-65 -0.9287827775729269 1102 66-67 -0.7049266971900252 1102 68-69 -1.0155173975498712 1102 70-71 -0.5892731689378508 1102 72-73 -0.8878029258528137 1102 74-75 -0.8898506971094697 1102 76-77 -0.7081903501343625 1102 78-79 -0.7710724957777693 1102 80-81 -0.8127430563903637 1102 82-83 -0.9573953878455761 1102 84-85 -0.915294960317734 1102 86-87 -0.8255582106012618 1102 88-89 -0.5870352522444691 1102 90-91 -0.3948653923063148 1102 92-93 -0.571679340659383 1102 94-95 -0.578490605298601 1102 96-97 -0.33618421850611924 1102 98-99 -0.30051448082543075 1102 100-101 -0.3510705611324916 1103 1 0.012196899245839887 1103 2 -0.0860083609964164 1103 3 -0.024094095589823894 1103 4 0.042748108396018836 1103 5 -0.10233770316334301 1103 6 0.01286989845948483 1103 7 -0.05831301876029471 1103 8 0.03851245241559553 1103 9 -0.07906361366571701 1103 10-11 0.0854202843495031 1103 12-13 0.021799779151741916 1103 14-15 -0.11467644937869181 1103 16-17 0.0012347768894329647 1103 18-19 -0.2740454866725486 1103 20-21 -0.28641497815117845 1103 22-23 0.0983230745142194 1103 24-25 0.19850523136350517 1103 26-27 -0.03003062869728268 1103 28-29 0.11022870955333275 1103 30-31 0.07924904627484608 1103 32-33 0.344186243709629 1103 34-35 -0.17415491476617717 1103 36-37 -0.23435547084028485 1103 38-39 -0.17593598750863038 1103 40-41 -0.21138937684251147 1103 42-43 0.142211601013436 1103 44-45 0.4147761402207877 1103 46-47 0.3327318225783529 1103 48-49 0.3531254789781215 1103 50-51 0.11677818894252567 1103 52-53 0.4507237812203897 1103 54-55 0.39702066281983406 1103 56-57 0.1328741595092353 1103 58-59 0.3193886115923519 1103 60-61 0.10522425737678276 1103 62-63 -0.1908568883689412 1103 64-65 0.01980707767986445 1103 66-67 0.07647183460044715 1103 68-69 0.2984430354475123 1103 70-71 0.043419378032410805 1103 72-73 0.06936807493520902 1103 74-75 -0.12224047770753899 1103 76-77 -0.0167528319891197 1103 78-79 0.25027460720427186 1103 80-81 0.09881805679448874 1103 82-83 0.2296914190060697 1103 84-85 -0.1681339134926354 1103 86-87 0.2798210768608911 1103 88-89 0.4737150277107389 1103 90-91 0.5913359265937714 1103 92-93 0.4598519106367043 1103 94-95 0.17825942580753562 1103 96-97 0.245515005914271 1103 98-99 0.03588799107535934 1103 100-101 0.18473882047755907 1104 1 -5.635886390820133E-4 1104 2 -0.10108247355999822 1104 3 0.1553075343396202 1104 4 -0.10181788186816476 1104 5 0.05220476798471907 1104 6 0.11092273442732647 1104 7 0.1694340686979956 1104 8 -0.03062280984989485 1104 9 0.04402887662580923 1104 10-11 -0.1172652705375512 1104 12-13 -0.1630604735420107 1104 14-15 -0.14391125767054547 1104 16-17 -0.07611029777007872 1104 18-19 -0.3005810661726329 1104 20-21 0.05811819474167379 1104 22-23 0.029124912287009863 1104 24-25 0.11393760023076283 1104 26-27 -0.25589345208512526 1104 28-29 -0.298951950075832 1104 30-31 -0.354875550344957 1104 32-33 -0.21465803786341553 1104 34-35 -0.4897896574232945 1104 36-37 -0.4956719943867469 1104 38-39 -0.43790338891977854 1104 40-41 -0.3499306446869568 1104 42-43 -0.5965183667161469 1104 44-45 -0.22729026026053134 1104 46-47 -0.6742090514576375 1104 48-49 -0.689416773387471 1104 50-51 -0.6653609567119076 1104 52-53 -0.2909241977764907 1104 54-55 -0.6706870437928742 1104 56-57 -0.661617991669452 1104 58-59 -0.9207552378853094 1104 60-61 -1.0691672538631707 1104 62-63 -0.9960064808865852 1104 64-65 -0.873254783065093 1104 66-67 -0.5156574658425868 1104 68-69 -0.7187149801954291 1104 70-71 -0.8661714987383604 1104 72-73 -0.5449485033068733 1104 74-75 -0.6740126157592776 1104 76-77 -1.1077253200928219 1104 78-79 -0.7433861705674296 1104 80-81 -0.7151594086105355 1104 82-83 -0.6887328002139732 1104 84-85 -0.6303337712840253 1104 86-87 -0.9908894192283704 1104 88-89 -0.6818803341007822 1104 90-91 -0.747689397477771 1104 92-93 -0.7878782211797386 1104 94-95 -0.7916853316173977 1104 96-97 -0.6290980225821485 1104 98-99 -0.638618380964239 1104 100-101 -0.8706514327631574 1105 1 -0.10651398936492384 1105 2 -0.4253074849012961 1105 3 -0.3060715674296226 1105 4 -0.1297174735814508 1105 5 -0.35872068188674433 1105 6 -0.12367578364314369 1105 7 -0.28165469585666614 1105 8 -0.2587341057821533 1105 9 -0.36385710553054906 1105 10-11 -0.2882655729721435 1105 12-13 -0.1720049767931826 1105 14-15 -0.5360782015689267 1105 16-17 -0.3064936336237096 1105 18-19 -0.3572119447451385 1105 20-21 -0.5312209856605605 1105 22-23 -0.16438030470981602 1105 24-25 -0.1442356947941832 1105 26-27 -0.38412950228926235 1105 28-29 -0.43914550821887843 1105 30-31 -0.6420901276926045 1105 32-33 -0.28991888770463703 1105 34-35 -0.1511574178950852 1105 36-37 -0.4534105396763266 1105 38-39 -0.36548542430463016 1105 40-41 -0.2719025182692931 1105 42-43 -0.02496612113925778 1105 44-45 0.29018289866885283 1105 46-47 -0.5452093538922327 1105 48-49 -0.6763175748391532 1105 50-51 -0.6390415857758711 1105 52-53 -0.3036566943740979 1105 54-55 -0.2944999879936816 1105 56-57 -0.2948479931816266 1105 58-59 -0.3551420517368129 1105 60-61 -0.45835521696614734 1105 62-63 -0.5113512563288864 1105 64-65 -0.9801616332087519 1105 66-67 -0.6209311691542538 1105 68-69 -0.6317196679317121 1105 70-71 -0.7042026495020046 1105 72-73 -0.6006569563538982 1105 74-75 -0.7813768982331943 1105 76-77 -0.9362146592732259 1105 78-79 -0.7134300235842126 1105 80-81 -0.8456750596010032 1105 82-83 -1.0712785434469971 1105 84-85 -1.0138473052323107 1105 86-87 -0.9229323649413637 1105 88-89 -0.7872674503868851 1105 90-91 -0.8121684538818243 1105 92-93 -0.8631768753457756 1105 94-95 -1.2744314377719412 1105 96-97 -1.1405300504061273 1105 98-99 -1.249906752353926 1105 100-101 -1.190355046856606 1106 1 -0.12616851784298078 1106 2 -0.1878658415772989 1106 3 -0.05779892503932871 1106 4 -0.2728547284834164 1106 5 -0.20395877712483923 1106 6 -0.2245161197775971 1106 7 -0.1386574969771175 1106 8 -0.05098433920885981 1106 9 -0.3869663492280253 1106 10-11 -0.042070428247590996 1106 12-13 -0.10099657343184276 1106 14-15 -0.18080275805819213 1106 16-17 -0.21109213969280205 1106 18-19 -0.25048925566951397 1106 20-21 -0.020389986594267384 1106 22-23 -0.11820850265565497 1106 24-25 0.05455048634492954 1106 26-27 -0.09489047334435696 1106 28-29 -0.5155227257725699 1106 30-31 -0.4517539932388246 1106 32-33 -0.08291608658418426 1106 34-35 -0.333813813786783 1106 36-37 -0.1603433127855709 1106 38-39 -0.3746357511020122 1106 40-41 0.03790140329932967 1106 42-43 -0.330871816750836 1106 44-45 -0.16485911813787624 1106 46-47 -0.2371094472629096 1106 48-49 -0.3374520286206675 1106 50-51 -0.42349536728847426 1106 52-53 -0.019809822291932733 1106 54-55 -0.47393509536997414 1106 56-57 -0.4743531285691134 1106 58-59 -0.15766306014017317 1106 60-61 -0.6085886436701671 1106 62-63 -0.39928309573132026 1106 64-65 -0.4586630337689712 1106 66-67 -0.823055352160786 1106 68-69 -0.7182742897804459 1106 70-71 -0.5290392508091948 1106 72-73 -0.8137288517787375 1106 74-75 -1.1145234902033145 1106 76-77 -1.0281847806551134 1106 78-79 -1.0464832448727286 1106 80-81 -1.0873183835972746 1106 82-83 -0.61710020544513 1106 84-85 -0.6288566423004731 1106 86-87 -0.7687773696098965 1106 88-89 -0.8927996632720365 1106 90-91 -0.7136623847875185 1106 92-93 -0.9742413011160842 1106 94-95 -0.8669617832434646 1106 96-97 -0.8958054939168676 1106 98-99 -0.6197013368543942 1106 100-101 -0.553660368985458 1107 1 -0.13654134914604654 1107 2 -0.16807994272163285 1107 3 0.014995464034129213 1107 4 0.027145271516587854 1107 5 -0.18158889894100838 1107 6 -0.19029685067460633 1107 7 0.04417749748578359 1107 8 -0.07017961421808394 1107 9 -0.1315953636244842 1107 10-11 -0.05567323371085564 1107 12-13 -0.08438527775077631 1107 14-15 0.060319427250043134 1107 16-17 -0.042118348625983515 1107 18-19 -0.1064139511290918 1107 20-21 -0.01660630257802609 1107 22-23 0.010676694834195644 1107 24-25 0.01663981820170335 1107 26-27 -0.24629320497964358 1107 28-29 -0.17462202440675156 1107 30-31 -0.02644945281800659 1107 32-33 0.01990783368159299 1107 34-35 0.18519689127043648 1107 36-37 0.35781837049238874 1107 38-39 0.30624280186956554 1107 40-41 0.2805370622140586 1107 42-43 0.54301153503949 1107 44-45 0.6939448685730909 1107 46-47 0.819553528004711 1107 48-49 0.678162589319534 1107 50-51 0.6829830380271389 1107 52-53 0.45863240731308963 1107 54-55 0.7084753882033041 1107 56-57 0.4359089607627453 1107 58-59 0.3515838944556151 1107 60-61 0.1938107657059902 1107 62-63 0.10069844727347999 1107 64-65 0.2571730681136444 1107 66-67 0.6844381878908976 1107 68-69 0.7349925983701624 1107 70-71 0.506878061852305 1107 72-73 0.7799403988672573 1107 74-75 0.21611512183064363 1107 76-77 0.3291980174253588 1107 78-79 0.390947541395267 1107 80-81 0.7934494274031003 1107 82-83 1.0035162581945904 1107 84-85 0.6486811945396909 1107 86-87 0.7183581047348788 1107 88-89 0.30902757925287716 1107 90-91 0.06797290498730746 1107 92-93 -0.13337751374066897 1107 94-95 0.32253618648706706 1107 96-97 -0.18847806682199675 1107 98-99 -0.2836732822216881 1107 100-101 0.34473387243203746 1108 1 -0.1285902506994958 1108 2 -0.1291670524437265 1108 3 -0.2535953990966746 1108 4 0.02385146387495496 1108 5 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0.08757833776374824 2316 74-75 0.12366511857352691 2316 76-77 0.24697861803769783 2316 78-79 0.41682207725612486 2316 80-81 0.3988627555250446 2316 82-83 0.28691473386392374 2316 84-85 0.4157745435071867 2316 86-87 0.23757183673930982 2316 88-89 0.5646233059156387 2316 90-91 0.5869912099837222 2316 92-93 0.18430211625086557 2316 94-95 0.4457366294549523 2316 96-97 0.46871364707286034 2316 98-99 0.4026142560494357 2316 100-101 0.4365357094459128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 4.0 6 14.0 7 47.0 8 122.0 9 209.0 10 425.0 11 719.0 12 1242.0 13 1862.0 14 2077.0 15 2599.0 16 3360.0 17 4784.0 18 4563.0 19 3187.0 20 2875.0 21 2833.0 22 3002.0 23 3137.0 24 3456.0 25 4082.0 26 4727.0 27 5832.0 28 7300.0 29 8883.0 30 11281.0 31 14079.0 32 17908.0 33 23338.0 34 31793.0 35 45389.0 36 72690.0 37 98226.0 38 81761.0 39 17768.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.75907740364208 19.120755945488188 13.171146816778826 16.9490198340909 2 15.52836219253011 27.94722144422294 34.90184852628631 21.62256783696064 3 30.78027744754849 25.38914975286029 26.450439164896476 17.380133634694747 4 12.819821081419486 17.248303074236386 42.85931991169396 27.07255593265017 5 12.415359649411842 42.124122705855214 33.09849583182313 12.362021812909814 6 27.204561929552867 42.339121552604695 14.916059837226928 15.540256680615505 7 28.822407657583444 34.77771096246993 20.11557217700748 16.28430920293914 8 24.124765231144355 29.72297275033774 19.20573989258295 26.94652212593496 9 22.028938350522257 27.023336848001584 21.834327325447298 29.11339747602886 10-11 26.443931431019145 25.310244983360242 27.074203433391546 21.171620152229067 12-13 33.33213203070942 23.40511631355234 23.79907492833372 19.46367672740453 14-15 23.349891056769927 27.950837565438018 30.065757227528657 18.6335141502634 16-17 19.171142377014068 29.21389502125276 29.8351057695476 21.779856832185573 18-19 18.654341988203896 35.80379501795775 27.474443144749415 18.06741984908893 20-21 23.22167846859205 26.65731575196596 25.877420630507046 24.243585148934944 22-23 20.164173448878053 29.635243335859503 25.88089805265412 24.319685162608323 24-25 26.51261161817523 28.81015437081947 26.128538007842106 18.5486960031632 26-27 25.668894685192413 24.731726455205706 31.962369106915027 17.637009752686854 28-29 20.409193096424115 33.985044792504475 28.345689590364074 17.26007252070733 30-31 22.982935441782683 28.56755952411601 26.43920101083642 22.01030402326489 32-33 22.836536481287737 30.066273446960643 27.103430237826526 19.993759833925083 34-35 26.056565731455912 26.11258364071102 28.683949839257433 19.146900788575643 36-37 24.959755121630682 25.27965426997387 31.422006301168672 18.338584307226775 38-39 21.76633791484184 28.779023346427774 31.352889482311173 18.10174925641921 40-41 20.253659862656683 30.4257972306859 28.404158810803352 20.91638409585406 42-43 19.330305651672433 32.397532542428735 24.511039710001647 23.761122095897182 44-45 23.807336662406634 25.80354485546954 25.706020652063096 24.68309783006073 46-47 26.37773180504311 30.276272336622018 25.249328858714847 18.09666699962002 48-49 26.25149821449901 29.02036336077829 26.0950866802038 18.6330517445189 50-51 19.94369556061943 29.872141144149865 32.4353935389935 17.7487697562372 52-53 23.389094713343397 33.36322879190084 24.26670318649206 18.98097330826371 54-55 19.830283859049725 30.19612809954374 26.24154225244066 23.732045788965877 56-57 24.608250225534174 26.84587880061131 27.2444254954543 21.30144547840021 58-59 29.134022062397534 26.384666716096984 24.434434805532902 20.046876415972584 60-61 22.445259984183473 29.31154853697113 30.91173635824436 17.331455120601028 62-63 20.498929585354627 28.38086784420859 29.681429361386197 21.43877320905059 64-65 19.538813063046813 34.985504169419315 26.390038329181607 19.08564443835227 66-67 22.642038108953656 27.006633063539994 25.103657713730616 25.247671113775738 68-69 21.042786807572668 30.366170199616832 24.731578188410275 23.859464804400222 70-71 26.312184853178657 29.211844577002932 24.559000428434892 19.916970141383516 72-73 25.265247619361432 25.57580150911068 29.710101650795746 19.44884922073215 74-75 20.86722736839504 34.27464362352806 26.470400467892137 18.387728540184767 76-77 23.27152758399483 28.454396335931943 25.052900600415377 23.221175479657852 78-79 22.914864697887065 30.42104287655999 25.425367601631038 21.23872482392191 80-81 25.73142289335894 27.93920640886367 26.02169581580695 20.307674881970435 82-83 24.18656136250135 26.742384068454573 29.26124048169448 19.8098140873496 84-85 21.141917467430567 29.290350880805967 28.667587905547613 20.90014374621585 86-87 20.799673779065635 30.139498579476708 26.1094137599743 22.951413881483358 88-89 20.212259876766943 30.87261936142871 23.892982470592113 25.022138291212233 90-91 23.34704552826274 24.682980574676545 25.540825100009783 26.42914879705093 92-93 24.705970785512612 28.866862583173557 25.738960132295123 20.6882064990187 94-95 24.6628944482487 28.17991319435506 26.15784623147142 20.99934612592482 96-97 18.29259125391691 29.899012366274025 32.17434912043859 19.634047259370472 98-99 22.426265769666838 33.26615789078035 23.5876423273253 20.719934012227508 100-101 19.018284871626935 31.53295931523382 24.09718703788375 25.35156877525549 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40.0 1 31.0 2 47.0 3 79.0 4 78.0 5 64.0 6 77.5 7 137.5 8 238.5 9 314.5 10 325.5 11 339.5 12 396.5 13 425.5 14 468.5 15 572.0 16 626.0 17 645.5 18 694.0 19 748.5 20 813.5 21 915.0 22 992.5 23 1070.5 24 1229.5 25 1479.5 26 1879.0 27 2464.0 28 3280.0 29 4425.5 30 5676.0 31 6828.0 32 7984.0 33 8921.0 34 9582.5 35 10139.5 36 10755.5 37 11220.5 38 11621.0 39 11815.0 40 11843.0 41 12263.0 42 12651.5 43 13168.0 44 13355.5 45 13660.5 46 16087.0 47 32408.5 48 67703.5 49 64693.5 50 30872.0 51 17443.5 52 12267.5 53 9512.0 54 7735.0 55 6570.5 56 5628.5 57 4557.5 58 3548.5 59 2754.5 60 2114.5 61 1623.5 62 1226.0 63 860.0 64 564.0 65 402.0 66 272.0 67 158.5 68 99.0 69 59.0 70 23.0 71 10.5 72 8.0 73 5.5 74 3.5 75 4.0 76 4.0 77 1.0 78 0.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.053131898909354505 2 0.0016475007413753334 3 0.04757158390721276 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002059375926719167 16-17 0.0 18-19 0.0 20-21 0.014415631487034168 22-23 0.0 24-25 0.0 26-27 0.005251408613133876 28-29 0.013900787505354378 30-31 0.00937016046657221 32-33 0.004118751853438334 34-35 0.005148439816797918 36-37 0.02440360473162213 38-39 0.054264555669050044 40-41 0.002574219908398959 42-43 0.013180005931002667 44-45 0.013077037134666711 46-47 0.00628109657649346 48-49 0.0012356255560315003 50-51 0.0019564071303832084 52-53 6.178127780157502E-4 54-55 0.001750469537711292 56-57 0.014415631487034168 58-59 0.010708754818939669 60-61 0.003295001482750667 62-63 0.005045471020461959 64-65 0.09153925994266697 66-67 0.0437617384427823 68-69 0.03171438927147517 70-71 0.019770008896504005 72-73 0.0 74-75 4.1187518534383335E-4 76-77 7.207815743517084E-4 78-79 8.237503706876667E-4 80-81 0.0013385943523674586 82-83 0.0013385943523674586 84-85 0.0012356255560315003 86-87 0.004839533427790042 88-89 0.0 90-91 0.0013385943523674586 92-93 6.178127780157502E-4 94-95 0.0033979702790866255 96-97 0.006075158983821543 98-99 0.00813453491054071 100-101 0.009473129262908168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 485584.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.63078879947286 #Duplication Level Percentage of deduplicated Percentage of total 1 88.70256838386746 47.57188710965995 2 7.096673077700159 7.6120035001808475 3 1.628870001415849 2.620727490831914 4 0.7500640176309049 1.609060996625886 5 0.441144725854143 1.1829469811142448 6 0.272502808379958 0.8768724338093253 7 0.1777395440511951 0.6672618363826974 8 0.13677910374408103 0.5868456980063999 9 0.10535755438293626 0.5085367872786207 >10 0.6018195593904313 5.816479391059948 >50 0.037566504362211345 1.4210080896628072 >100 0.0320880558087447 3.3066612701804914 >500 0.007043719567773226 2.524697580621434 >1k 0.0074350373215384055 9.6444287046931 >5k 0.0015652710150607172 5.081504781345535 >10k+ 7.826355075303586E-4 8.969077348546818 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 32404 6.6732017529407885 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10333 2.1279531450789153 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6430 1.3241787208804245 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6242 1.285462453458104 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6094 1.2549836897426603 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5447 1.1217420672839302 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4607 0.9487544894395202 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4582 0.9436060496227224 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4528 0.9324854196184389 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2710 0.5580908761408943 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2708 0.5576790009555505 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2690 0.5539721242874559 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2680 0.5519127483607367 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2579 0.5311130515008732 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2421 0.4985749118587103 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2364 0.4868364690764111 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2294 0.4724208375893769 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2171 0.44709051369073116 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1573 0.32393983327292497 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1486 0.30602326271046826 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1451 0.29881544696695117 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1425 0.2934610695574813 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1396 0.28748887936999573 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1247 0.25680417806188016 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1043 0.2147929091568091 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 962 0.19811196415038387 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 818 0.16845695080562786 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 815 0.1678391380276121 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 780 0.16063132228409502 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 773 0.1591897591353916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 710 0.14621569079706087 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 699 0.1439503772776698 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 686 0.14127318857293486 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 679 0.13983162542423144 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 640 0.1318000593100267 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 626 0.12891693301261983 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 580 0.11944380374971168 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 574 0.1182081781936802 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 572 0.11779630300833635 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 557 0.1147072391182576 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 528 0.10873504893077202 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 522 0.10749942337474053 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 509 0.10482223467000559 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 490 0.10090942040923917 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 488 0.10049754522389535 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 2.059375926719167E-4 0.0 0.0 0.0 8 0.0 2.059375926719167E-4 0.0 0.0 0.0 9 0.0 2.059375926719167E-4 0.0 0.0 0.0 10-11 0.0 2.059375926719167E-4 0.0 4.118751853438334E-4 0.0 12-13 0.0 2.059375926719167E-4 0.0 8.237503706876668E-4 0.0 14-15 0.0 2.059375926719167E-4 0.0 0.002368282315727042 0.0 16-17 0.0 2.059375926719167E-4 0.0 0.002677188704734917 0.0 18-19 1.0296879633595835E-4 2.059375926719167E-4 0.0 0.0033979702790866255 0.0 20-21 2.059375926719167E-4 2.059375926719167E-4 0.0 0.0046335958351181265 0.0 22-23 2.059375926719167E-4 2.059375926719167E-4 0.0 0.0074137533361890015 0.0 24-25 2.059375926719167E-4 2.059375926719167E-4 0.0 0.00998797324458796 0.0 26-27 2.059375926719167E-4 2.059375926719167E-4 0.0 0.01297406833833075 0.0 28-29 2.059375926719167E-4 2.059375926719167E-4 0.0 0.021520478434215295 0.0 30-31 2.059375926719167E-4 2.059375926719167E-4 0.0 0.03675986029193713 0.0 32-33 2.059375926719167E-4 2.059375926719167E-4 0.0 0.06033971465287159 0.0 34-35 2.059375926719167E-4 2.059375926719167E-4 0.0 0.0870086329038848 0.0 36-37 2.059375926719167E-4 2.059375926719167E-4 0.0 0.10471926587366964 0.0 38-39 2.059375926719167E-4 2.059375926719167E-4 0.0 0.1176933342120004 0.0 40-41 2.059375926719167E-4 2.059375926719167E-4 0.0 0.13756631190484037 0.0 42-43 2.059375926719167E-4 2.059375926719167E-4 0.0 0.17587070414181688 0.0 44-45 2.059375926719167E-4 2.059375926719167E-4 0.0 0.23075307258888267 0.0 46-47 2.059375926719167E-4 2.059375926719167E-4 0.0 0.3577135984711193 0.0 48-49 2.059375926719167E-4 2.059375926719167E-4 0.0 0.7855489472470263 0.0 50-51 2.059375926719167E-4 2.059375926719167E-4 0.0 0.9552415236086856 0.0 52-53 2.059375926719167E-4 2.059375926719167E-4 0.0 1.09805924412666 0.0 54-55 2.059375926719167E-4 2.059375926719167E-4 0.0 1.2550866585389964 0.0 56-57 2.059375926719167E-4 2.059375926719167E-4 0.0 1.3691760848792383 0.0 58-59 2.059375926719167E-4 2.059375926719167E-4 0.0 1.495621766779795 0.0 60-61 2.059375926719167E-4 2.059375926719167E-4 0.0 1.866721308774589 0.0 62-63 2.059375926719167E-4 2.059375926719167E-4 0.0 1.9886363636363635 0.0 64-65 2.059375926719167E-4 2.059375926719167E-4 0.0 2.1396915878612144 0.0 66-67 2.059375926719167E-4 2.059375926719167E-4 0.0 2.741647171241227 0.0 68-69 2.059375926719167E-4 2.059375926719167E-4 0.0 3.3052983623842627 0.0 70-71 2.059375926719167E-4 2.059375926719167E-4 0.0 3.6492141421463637 0.0 72-73 2.059375926719167E-4 2.059375926719167E-4 0.0 3.9945714850571683 0.0 74-75 2.059375926719167E-4 2.059375926719167E-4 0.0 4.196287357079311 0.0 76-77 2.059375926719167E-4 2.059375926719167E-4 0.0 4.2870028666512905 0.0 78-79 2.059375926719167E-4 2.059375926719167E-4 0.0 4.465756697090514 0.0 80-81 2.059375926719167E-4 2.059375926719167E-4 0.0 4.808951695278263 0.0 82-83 2.059375926719167E-4 2.059375926719167E-4 0.0 5.2613965863784635 0.0 84-85 2.059375926719167E-4 2.059375926719167E-4 0.0 5.966217997298099 0.0 86-87 2.059375926719167E-4 2.059375926719167E-4 0.0 7.8384996210748294 0.0 88-89 2.059375926719167E-4 2.059375926719167E-4 0.0 10.022467791360507 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 535 0.0 53.275707 2 ACCTGGG 560 0.0 51.745613 3 GTACCCG 70 7.8773155E-8 47.523754 1 GTACCTG 670 0.0 42.55859 1 CCTGGGG 430 0.0 40.858845 4 TACCCGG 85 3.6324127E-7 39.121082 2 GTGTCGG 50 0.0016109339 38.019005 1 ACGTCTT 95 8.7082117E-7 34.98866 8 CAAGACG 130 8.292773E-9 32.87396 4 TACGCAG 360 0.0 30.337387 4 GACGTCT 110 2.7360693E-6 30.217476 7 ACCCGGG 95 3.484642E-5 30.002638 3 AGTACGC 365 0.0 29.934137 2 GTACGCA 370 0.0 29.52962 3 GAGTACG 390 0.0 29.245388 1 CCCCTAT 135 4.1982275E-7 28.162226 1 GTACTAG 135 4.1982275E-7 28.162226 1 ACGCAGT 405 0.0 28.139025 5 CTGGGGC 190 3.965397E-10 27.491087 5 AAGACGT 140 5.8109254E-7 27.13406 5 >>END_MODULE