##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003838_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 692429 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.11615053673373 31.0 31.0 34.0 28.0 34.0 2 30.895130042213715 33.0 31.0 34.0 27.0 34.0 3 31.094630640831046 33.0 31.0 34.0 27.0 34.0 4 34.457036028242605 37.0 35.0 37.0 32.0 37.0 5 34.33786135473817 37.0 35.0 37.0 30.0 37.0 6 34.43554646035911 37.0 35.0 37.0 31.0 37.0 7 34.41150356209806 37.0 35.0 37.0 30.0 37.0 8 34.50891860392907 37.0 35.0 37.0 32.0 37.0 9 36.021105412973746 39.0 37.0 39.0 32.0 39.0 10-11 35.93299457417295 39.0 37.0 39.0 31.5 39.0 12-13 35.92783158995363 39.0 37.0 39.0 31.0 39.0 14-15 36.92981951940199 40.0 37.0 41.0 31.0 41.0 16-17 36.913632300206956 40.0 37.0 41.0 31.0 41.0 18-19 36.85249823447603 40.0 37.0 41.0 30.5 41.0 20-21 36.82640386234546 40.0 37.0 41.0 30.5 41.0 22-23 36.63974934614235 40.0 37.0 41.0 30.0 41.0 24-25 36.5313432857376 39.5 36.5 41.0 30.0 41.0 26-27 36.385461902953224 39.0 36.0 41.0 29.5 41.0 28-29 36.24726578465085 39.0 36.0 41.0 29.5 41.0 30-31 35.92090091547293 39.0 36.0 40.0 27.0 41.0 32-33 35.687497923974874 39.0 35.0 40.0 27.0 41.0 34-35 35.514619549441164 39.0 35.0 40.0 26.0 41.0 36-37 35.482580163453584 38.5 35.0 40.0 26.5 41.0 38-39 35.390004606970535 38.5 35.0 40.0 26.0 41.0 40-41 35.59266076377506 39.0 35.0 41.0 27.0 41.0 42-43 35.470104516130895 39.0 35.0 41.0 26.0 41.0 44-45 35.25330972561808 39.0 35.0 41.0 24.5 41.0 46-47 34.90581344802139 39.0 35.0 40.0 24.0 41.0 48-49 34.58930013041048 38.5 34.0 40.0 23.0 41.0 50-51 33.77545784477542 37.5 33.5 39.5 21.5 40.5 52-53 33.87103443096693 38.0 33.5 40.0 21.5 40.5 54-55 34.226765487869514 38.0 34.0 40.0 21.0 41.0 56-57 33.793885004816374 38.0 33.5 40.0 16.0 41.0 58-59 33.482102136103485 38.0 33.0 40.0 13.5 41.0 60-61 32.897310049116946 38.0 32.0 40.0 10.0 41.0 62-63 32.79082620745231 37.0 32.0 40.0 8.0 41.0 64-65 32.467858076423724 37.0 31.5 39.5 8.0 41.0 66-67 31.963520447583797 36.0 31.0 39.0 6.5 41.0 68-69 31.591290948241625 36.0 30.5 39.0 2.0 40.5 70-71 30.929326328042297 35.0 30.0 38.5 2.0 40.0 72-73 30.49356612735746 35.0 29.5 37.0 2.0 40.0 74-75 30.048851940054504 35.0 29.0 37.0 2.0 39.0 76-77 29.585075870594675 34.0 29.0 36.5 2.0 39.0 78-79 28.895195030826265 34.0 28.5 36.0 2.0 38.5 80-81 28.372337813696422 34.0 26.0 35.5 2.0 37.5 82-83 27.893124060372976 34.0 26.0 35.0 2.0 37.0 84-85 27.583054724744343 33.5 26.0 35.0 2.0 36.5 86-87 27.50016608200985 34.0 26.0 35.0 2.0 36.0 88-89 27.081805499191972 33.5 25.0 35.0 2.0 36.0 90-91 26.743895041946537 33.0 25.0 35.0 2.0 35.5 92-93 26.41966252135598 33.0 24.5 35.0 2.0 35.0 94-95 26.111958049128503 33.0 23.0 35.0 2.0 35.0 96-97 25.838841960691997 32.5 21.5 35.0 2.0 35.0 98-99 25.490541990586763 32.0 19.5 35.0 2.0 35.0 100-101 25.132227997383126 32.0 18.5 35.0 2.0 35.0 102-103 24.678662361050733 32.0 16.5 35.0 2.0 35.0 104-105 24.161581187385277 31.5 8.5 34.0 2.0 35.0 106-107 23.54840496281929 31.0 6.0 34.0 2.0 35.0 108-109 23.296501879615093 31.0 2.0 34.0 2.0 35.0 110-111 22.95469716028647 31.0 2.0 34.0 2.0 35.0 112-113 22.269583596296513 30.5 2.0 34.0 2.0 35.0 114-115 21.64382052167081 29.5 2.0 34.0 2.0 35.0 116-117 20.83405663252117 29.0 2.0 34.0 2.0 35.0 118-119 20.038814087798173 29.0 2.0 34.0 2.0 35.0 120-121 18.4858628104831 26.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6905480364084653 1101 2 0.7295024943488038 1101 3 0.7336465332292406 1101 4 0.8138522852997028 1101 5 0.965035441933189 1101 6 0.9278300986876573 1101 7 0.9984125956250196 1101 8 0.8420959512951924 1101 9 1.0256276149085508 1101 10-11 1.080650104744734 1101 12-13 0.8292089160519609 1101 14-15 0.9635908767566121 1101 16-17 1.032743821001631 1101 18-19 1.121810717322937 1101 20-21 1.0277649371413986 1101 22-23 1.2868178432355037 1101 24-25 1.3858924732668996 1101 26-27 1.3258602191073905 1101 28-29 1.097896420969228 1101 30-31 1.2306949828838114 1101 32-33 1.1080992266908751 1101 34-35 1.316715560642983 1101 36-37 1.0148013341654405 1101 38-39 1.305534032743573 1101 40-41 1.229200035019126 1101 42-43 1.0441732609618413 1101 44-45 1.0580551580474165 1101 46-47 1.1866563337835387 1101 48-49 1.1914324308340696 1101 50-51 0.7668873445955313 1101 52-53 1.0900931688264208 1101 54-55 1.2048583531828854 1101 56-57 1.178291415537963 1101 58-59 1.162470067654091 1101 60-61 1.2134713068069019 1101 62-63 1.2581986077843723 1101 64-65 1.5157138572734894 1101 66-67 1.268916299007092 1101 68-69 1.0587204154125978 1101 70-71 0.7688459686367253 1101 72-73 0.7679113588369155 1101 74-75 0.877081037945505 1101 76-77 1.0130611110642356 1101 78-79 1.0472518891003446 1101 80-81 0.9124969242103873 1101 82-83 1.0016180158349606 1101 84-85 1.0119634035382319 1101 86-87 1.0827559563062117 1101 88-89 1.0120486374495243 1101 90-91 0.9456271569706338 1101 92-93 1.2544500040018036 1101 94-95 1.0152369701342643 1101 96-97 1.1409901539984446 1101 98-99 1.1582818695371522 1101 100-101 1.1976331950193746 1101 102-103 0.8663972860022646 1101 104-105 0.830515592401408 1101 106-107 0.8621046997580706 1101 108-109 0.7788231582155625 1101 110-111 0.8928859972152203 1101 112-113 0.7659593927685329 1101 114-115 0.06982990989053661 1101 116-117 0.16657333665169105 1101 118-119 0.2757759974618317 1101 120-121 0.17712800285215735 1102 1 0.7132299496587784 1102 2 0.9335098487977014 1102 3 1.0127294143140908 1102 4 1.1988091565148267 1102 5 1.2231930021152735 1102 6 1.266021970517933 1102 7 1.105089697042601 1102 8 1.0258717362937588 1102 9 0.9597690718549501 1102 10-11 1.145614972897782 1102 12-13 1.049363933617883 1102 14-15 1.1859638484734276 1102 16-17 1.2768828173517477 1102 18-19 1.1317505010638556 1102 20-21 1.198294512141743 1102 22-23 1.242195613115065 1102 24-25 1.234487199388866 1102 26-27 1.177894653785394 1102 28-29 1.2172507194942384 1102 30-31 1.2455593766885826 1102 32-33 1.1872501159230353 1102 34-35 1.0997849494582042 1102 36-37 0.9880953207415217 1102 38-39 1.109033205709821 1102 40-41 1.2305721591972159 1102 42-43 1.3634663863292218 1102 44-45 1.241926632901496 1102 46-47 1.1429311960584911 1102 48-49 1.2489078590461702 1102 50-51 0.8518291788333627 1102 52-53 1.0373646409464996 1102 54-55 1.0807930380215112 1102 56-57 1.059487333682121 1102 58-59 0.9453514942531385 1102 60-61 1.0930729636083996 1102 62-63 0.7281041482270112 1102 64-65 1.0049470397813778 1102 66-67 0.8372576520616555 1102 68-69 0.8193078144256241 1102 70-71 0.4432475652903136 1102 72-73 0.3044136212185009 1102 74-75 0.22275209723220613 1102 76-77 0.34408820496332737 1102 78-79 0.4801869703683721 1102 80-81 0.2238136166221807 1102 82-83 -0.031680345134514454 1102 84-85 0.0563993801899052 1102 86-87 0.47988936163476836 1102 88-89 0.5517923936864548 1102 90-91 0.36453342192217164 1102 92-93 0.40629784346156583 1102 94-95 0.3651935679498024 1102 96-97 0.5279394247050462 1102 98-99 0.6450766694770032 1102 100-101 0.2607987521393831 1102 102-103 0.1624787864192001 1102 104-105 -0.11050186416223085 1102 106-107 0.11130842346045 1102 108-109 0.28162379523660164 1102 110-111 0.16601731103693496 1102 112-113 0.2870111609803594 1102 114-115 0.2116849944393593 1102 116-117 0.034459821143101976 1102 118-119 0.12740375025392225 1102 120-121 0.15536539571369445 1103 1 0.6070378582963549 1103 2 0.8336113712454427 1103 3 0.76885965866812 1103 4 1.1451714632915468 1103 5 1.0583571106237102 1103 6 1.2897980610090514 1103 7 0.9483542077510805 1103 8 1.1954667356325004 1103 9 1.1434721906556575 1103 10-11 0.8576811686455059 1103 12-13 0.9625330676819743 1103 14-15 0.9766253391808206 1103 16-17 0.9194926833109918 1103 18-19 1.015006493222124 1103 20-21 0.9909650581846989 1103 22-23 1.0929109379479982 1103 24-25 0.8906119980943572 1103 26-27 1.0767893816196619 1103 28-29 0.951805498828783 1103 30-31 1.0365232953772647 1103 32-33 1.0399929723990766 1103 34-35 1.1537484091565133 1103 36-37 0.9170928157431888 1103 38-39 0.9602318318504928 1103 40-41 0.9873665781854228 1103 42-43 0.9735307227769496 1103 44-45 0.9094192229297349 1103 46-47 1.1927793013617745 1103 48-49 1.3517178136916996 1103 50-51 1.092880811360999 1103 52-53 0.943757832329922 1103 54-55 0.696620037546964 1103 56-57 1.101569555195546 1103 58-59 1.0865347947829278 1103 60-61 1.4459801619778148 1103 62-63 1.2249359477026687 1103 64-65 1.261253295275658 1103 66-67 0.9719547962079709 1103 68-69 1.0496237398357984 1103 70-71 0.9360690335927515 1103 72-73 0.7423235035146973 1103 74-75 0.9198894364883294 1103 76-77 0.8597454169468648 1103 78-79 0.8893049308952001 1103 80-81 0.794869605668648 1103 82-83 0.8556929462932565 1103 84-85 0.7138690595967994 1103 86-87 0.6445113169337766 1103 88-89 1.130825285403624 1103 90-91 0.7989911520202746 1103 92-93 0.6100166379505723 1103 94-95 0.6468240340195202 1103 96-97 0.3698308540321982 1103 98-99 0.5514795852328227 1103 100-101 0.6482566456069385 1103 102-103 0.5232938444153596 1103 104-105 0.3058704781667956 1103 106-107 0.1911708041423985 1103 108-109 0.48015366461883247 1103 110-111 0.5039752317327348 1103 112-113 -0.018015172620067688 1103 114-115 -0.12029827186063358 1103 116-117 -0.10677696343482168 1103 118-119 -0.04231085886520347 1103 120-121 -0.031101389719975003 1104 1 0.626409287465723 1104 2 0.9465804188146585 1104 3 0.6327887757181152 1104 4 1.0561021290094743 1104 5 1.127016466461555 1104 6 1.0466336510724616 1104 7 0.9326190090402378 1104 8 0.9548869269139217 1104 9 1.1607037374400804 1104 10-11 1.1188239914067424 1104 12-13 0.9368584030522982 1104 14-15 1.3428199062862731 1104 16-17 1.1924218631448298 1104 18-19 1.0528062860354694 1104 20-21 1.2041292588418386 1104 22-23 1.4847984816350888 1104 24-25 1.3714747003106211 1104 26-27 1.1207132143635192 1104 28-29 1.3619487506128891 1104 30-31 1.4433416198859845 1104 32-33 1.26330461042933 1104 34-35 1.0372019909743173 1104 36-37 0.9216040959055931 1104 38-39 0.9665612272421527 1104 40-41 0.9471260637765297 1104 42-43 0.9474388072421362 1104 44-45 0.8508419046366171 1104 46-47 1.1009265503833987 1104 48-49 1.2094104031063466 1104 50-51 1.1659376516598456 1104 52-53 1.2059823682527409 1104 54-55 0.9129495898982185 1104 56-57 1.082577445030843 1104 58-59 0.9917298955509821 1104 60-61 0.8156736199021779 1104 62-63 0.9294582150362416 1104 64-65 1.206102927990223 1104 66-67 0.9820616856874942 1104 68-69 0.8409184994783523 1104 70-71 0.5734923849938767 1104 72-73 0.9622513544240157 1104 74-75 0.7920039960597229 1104 76-77 0.4286689880914132 1104 78-79 0.8068959151364581 1104 80-81 0.703428873609127 1104 82-83 0.5499369765969213 1104 84-85 0.6474555338658909 1104 86-87 0.5071965292477785 1104 88-89 0.5121520648021551 1104 90-91 0.5880466215387194 1104 92-93 0.4519204007950961 1104 94-95 0.6126264637539443 1104 96-97 0.32812930745705415 1104 98-99 0.5354147713868933 1104 100-101 0.5903974174295534 1104 102-103 0.382102154902352 1104 104-105 0.26221216872619735 1104 106-107 0.4356484426330738 1104 108-109 -0.128168071144934 1104 110-111 -0.21880405169442696 1104 112-113 -0.42546395147447313 1104 114-115 -0.2596574581821294 1104 116-117 -0.41976092216491523 1104 118-119 0.1168265435582505 1104 120-121 0.04326083115355672 1105 1 0.9232734488796019 1105 2 0.9517980199245315 1105 3 0.9526797489665988 1105 4 1.1864516781242926 1105 5 1.2235797864241604 1105 6 1.1453873079171686 1105 7 1.077524308072121 1105 8 1.0744355893381439 1105 9 1.2050043376150086 1105 10-11 1.312250341800329 1105 12-13 0.9559022701090214 1105 14-15 1.0829615053452954 1105 16-17 1.1645808341011872 1105 18-19 1.1353058336423132 1105 20-21 1.1360026189166348 1105 22-23 1.078227093994471 1105 24-25 1.1167113773320665 1105 26-27 1.0703335811035757 1105 28-29 1.141599352295792 1105 30-31 1.122625029120833 1105 32-33 1.1684980839040122 1105 34-35 1.25594350860257 1105 36-37 1.2229224449721556 1105 38-39 1.00161227227035 1105 40-41 1.013399776717982 1105 42-43 1.2813728181598876 1105 44-45 1.3571603291020367 1105 46-47 1.2483313216232546 1105 48-49 1.3739344855022182 1105 50-51 1.0187854666008889 1105 52-53 1.2190075213124274 1105 54-55 1.2070164536072383 1105 56-57 1.2734421191569751 1105 58-59 1.028424635726502 1105 60-61 1.5509274878636958 1105 62-63 1.2248389038802188 1105 64-65 1.4857045204141386 1105 66-67 1.213375963215615 1105 68-69 1.2418500523932643 1105 70-71 0.7122112075652822 1105 72-73 0.6923554802496348 1105 74-75 0.6016143347514067 1105 76-77 0.974522804781703 1105 78-79 1.0091985963199193 1105 80-81 1.007721707701478 1105 82-83 0.7735191010351983 1105 84-85 0.8313175388723728 1105 86-87 1.0405360201546756 1105 88-89 1.1939594508744769 1105 90-91 0.6477380140048687 1105 92-93 0.7587466365824085 1105 94-95 0.77614208639773 1105 96-97 0.9168398361538621 1105 98-99 1.0736533032202935 1105 100-101 1.020745835561474 1105 102-103 0.8639065250202727 1105 104-105 0.4319463123459464 1105 106-107 0.30707453667053386 1105 108-109 0.022684312967019338 1105 110-111 -0.13706603257327643 1105 112-113 0.04110042407216952 1105 114-115 -0.017099325661092735 1105 116-117 0.0022446121114079176 1105 118-119 -0.28371293528482866 1105 120-121 -0.14017705526559965 1106 1 0.6817533773213142 1106 2 1.0455285379089716 1106 3 1.0075302626141749 1106 4 1.162859126691771 1106 5 1.3298601106048977 1106 6 1.004357844333292 1106 7 0.823709221199266 1106 8 1.2929106388055231 1106 9 1.3810594158141214 1106 10-11 1.3007793310394717 1106 12-13 1.0629035982742607 1106 14-15 1.2936979594151765 1106 16-17 1.2881029114600295 1106 18-19 1.4070918093016473 1106 20-21 1.1487584262513622 1106 22-23 1.2951679770888163 1106 24-25 0.9530868415122598 1106 26-27 1.2322504759074633 1106 28-29 1.1488581818840586 1106 30-31 1.201230726678638 1106 32-33 1.5794215653232584 1106 34-35 1.500664729430369 1106 36-37 0.8823212469129018 1106 38-39 1.127077807737784 1106 40-41 1.1911245483548853 1106 42-43 0.8381234212553252 1106 44-45 1.2666201182219297 1106 46-47 0.9972538814584553 1106 48-49 1.0206289803928499 1106 50-51 1.3615117290354988 1106 52-53 1.0570743632607602 1106 54-55 1.3800872258404482 1106 56-57 1.377906380669998 1106 58-59 1.682144853654023 1106 60-61 1.7872839782548269 1106 62-63 1.627066968824792 1106 64-65 1.3001571265995935 1106 66-67 0.5846713309005374 1106 68-69 0.8428150050943266 1106 70-71 0.7240183254467212 1106 72-73 1.0734142176794954 1106 74-75 1.4028882891600993 1106 76-77 1.3221036466758314 1106 78-79 1.8590888193974635 1106 80-81 1.7566090304998383 1106 82-83 1.7791868428832451 1106 84-85 1.3874203225982846 1106 86-87 1.3322255005439096 1106 88-89 1.1096805903860378 1106 90-91 0.9936871100393461 1106 92-93 0.787182925309267 1106 94-95 1.022955845105347 1106 96-97 1.1004193769881638 1106 98-99 0.9172252545389696 1106 100-101 0.9854789827709673 1106 102-103 1.2417451393915648 1106 104-105 1.170070075661478 1106 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1.6674412560900365 2205 24-25 1.4587801540375551 2205 26-27 1.320395669826894 2205 28-29 1.371677672086939 2205 30-31 1.167336732111849 2205 32-33 1.3329618231118232 2205 34-35 1.3837524122232665 2205 36-37 1.3575943525137362 2205 38-39 1.3738727906867467 2205 40-41 1.4397517953348213 2205 42-43 1.343834073818762 2205 44-45 1.35636637566369 2205 46-47 1.3704808625578053 2205 48-49 1.423033465682721 2205 50-51 1.5905678529589693 2205 52-53 1.3202903623359603 2205 54-55 1.271324726562959 2205 56-57 1.3737794515907567 2205 58-59 1.5033632803345611 2205 60-61 1.5949078150272769 2205 62-63 1.3609031632644175 2205 64-65 1.5197340296687827 2205 66-67 1.5070399801115748 2205 68-69 1.620954997002304 2205 70-71 1.5654725766460729 2205 72-73 1.3651430194828613 2205 74-75 1.3467181255857419 2205 76-77 1.6129943466353716 2205 78-79 1.7434481049878947 2205 80-81 1.695913116968832 2205 82-83 1.714057659599046 2205 84-85 1.8125344778494252 2205 86-87 1.8618133856372552 2205 88-89 1.8500250791439115 2205 90-91 1.8711508404595527 2205 92-93 0.897662088363429 2205 94-95 1.4062458283433088 2205 96-97 1.601939520332479 2205 98-99 1.7239011692259822 2205 100-101 1.8868835258609629 2205 102-103 1.69157462901644 2205 104-105 1.4092296618125708 2205 106-107 1.4619443459065984 2205 108-109 1.5984775330739893 2205 110-111 1.7800117885525353 2205 112-113 1.7757821093654549 2205 114-115 1.6076716305052479 2205 116-117 1.6074522426688382 2205 118-119 2.0398191797406433 2205 120-121 1.6822247516629183 2206 1 0.8053781624439864 2206 2 1.2686005022784066 2206 3 1.117813119310533 2206 4 1.3174262465542554 2206 5 1.4684462178091522 2206 6 1.393627652115761 2206 7 1.2840252862094275 2206 8 1.37508558801634 2206 9 1.2059676045936172 2206 10-11 1.2424655320083744 2206 12-13 1.1439582552519099 2206 14-15 1.4094767920452753 2206 16-17 1.5000422769858943 2206 18-19 1.5269738221161546 2206 20-21 1.5278283981219687 2206 22-23 1.617617773932075 2206 24-25 1.4584324033187954 2206 26-27 1.531576542480373 2206 28-29 1.4825636436749576 2206 30-31 1.4190088951424613 2206 32-33 1.5861941079080353 2206 34-35 1.618236811005616 2206 36-37 1.571050931238581 2206 38-39 1.6489425522291157 2206 40-41 1.5935516854075473 2206 42-43 1.5194154200051386 2206 44-45 1.6706178131742675 2206 46-47 1.6863410435150215 2206 48-49 1.810664532822912 2206 50-51 1.807532591670281 2206 52-53 1.6799216831544967 2206 54-55 1.7081482510006296 2206 56-57 1.9001852523687006 2206 58-59 1.8266879463247605 2206 60-61 1.9973050753012416 2206 62-63 1.6582173438833934 2206 64-65 1.7632832009862156 2206 66-67 1.9171490617959996 2206 68-69 1.9101673257694252 2206 70-71 1.792497775360772 2206 72-73 1.6735841661089417 2206 74-75 1.8866777484506123 2206 76-77 1.9715908712421708 2206 78-79 2.0730724496521944 2206 80-81 1.8406006698184854 2206 82-83 1.7566999308657465 2206 84-85 1.6466024213637134 2206 86-87 1.5865142565995427 2206 88-89 1.6705973351899104 2206 90-91 1.5862425874576935 2206 92-93 1.7150105155577435 2206 94-95 1.5852888268212375 2206 96-97 1.7596249169162803 2206 98-99 1.649427551772913 2206 100-101 1.8442391406081349 2206 102-103 1.53294005265926 2206 104-105 1.4538907898355689 2206 106-107 1.53742422075862 2206 108-109 1.7610631495123457 2206 110-111 1.7986222654678024 2206 112-113 1.6169308494297177 2206 114-115 1.4151382295243558 2206 116-117 0.5833961374768855 2206 118-119 0.8648540605118242 2206 120-121 0.7269408433253552 2207 1 0.5552607146126753 2207 2 0.9509054842515283 2207 3 0.9381452508521058 2207 4 1.3320088970513595 2207 5 1.3187589568018296 2207 6 1.1588890418240538 2207 7 1.2858129514557959 2207 8 1.2972682057373035 2207 9 1.3446530082864 2207 10-11 1.2100881693950285 2207 12-13 1.2246824713559192 2207 14-15 1.455168778279294 2207 16-17 1.3893463303017484 2207 18-19 1.476140761917307 2207 20-21 1.5553787914356292 2207 22-23 1.6117132890637365 2207 24-25 1.2691672716679676 2207 26-27 1.3925114818445081 2207 28-29 1.430660579105968 2207 30-31 1.4963018591979633 2207 32-33 1.4959108674404291 2207 34-35 1.4032355819004039 2207 36-37 1.22092324086384 2207 38-39 1.552035800344484 2207 40-41 1.7351370945630151 2207 42-43 1.6626949458436613 2207 44-45 1.847470347862341 2207 46-47 1.936119121740738 2207 48-49 1.8097126591203079 2207 50-51 1.8027349843284242 2207 52-53 1.8279660401959035 2207 54-55 1.6106406032348772 2207 56-57 1.9322471446444567 2207 58-59 1.5561898855796485 2207 60-61 1.9047534528823675 2207 62-63 1.9843706542734267 2207 64-65 2.0410275197887984 2207 66-67 2.166501733051323 2207 68-69 1.9346879747369776 2207 70-71 1.8554006487356247 2207 72-73 1.9997108458860602 2207 74-75 2.043568248833001 2207 76-77 2.114693542497495 2207 78-79 2.2987942075457823 2207 80-81 2.340847856533223 2207 82-83 2.1919458884752707 2207 84-85 2.179455720828372 2207 86-87 2.1892995455758353 2207 88-89 2.2923231354739713 2207 90-91 1.8650268658363665 2207 92-93 2.0804337374522035 2207 94-95 2.067214834687512 2207 96-97 2.217076708678544 2207 98-99 2.035411655345513 2207 100-101 1.9741425875654137 2207 102-103 2.234060586910328 2207 104-105 2.4000689554643273 2207 106-107 1.9715919799632502 2207 108-109 2.193493090515293 2207 110-111 2.01778606422231 2207 112-113 2.06489190045124 2207 114-115 2.1944619485235926 2207 116-117 1.906945110491197 2207 118-119 2.140257616722902 2207 120-121 2.220089947444226 2208 1 0.4217790999233557 2208 2 1.1480692089529931 2208 3 1.0803139445564582 2208 4 1.3129121513538848 2208 5 1.3319134060312408 2208 6 1.2117970916087302 2208 7 1.1283302640928383 2208 8 1.0256640854085575 2208 9 1.195177514146664 2208 10-11 1.2983841957701756 2208 12-13 1.1662667156731175 2208 14-15 1.476117463465357 2208 16-17 1.472627607414367 2208 18-19 1.4522643086811442 2208 20-21 1.4171072157096063 2208 22-23 1.4015828330196882 2208 24-25 1.390710659739213 2208 26-27 1.3558362968871833 2208 28-29 1.435481300636738 2208 30-31 1.437523287431361 2208 32-33 1.3639925168338962 2208 34-35 1.3869719565789964 2208 36-37 1.4714004228870863 2208 38-39 1.415641403486788 2208 40-41 1.600324891322913 2208 42-43 1.5091369820306184 2208 44-45 1.444374580190832 2208 46-47 1.46675341890289 2208 48-49 1.8222716167339286 2208 50-51 1.7273063092381165 2208 52-53 1.9409469686895804 2208 54-55 1.8785574079293994 2208 56-57 1.9112724313480385 2208 58-59 1.9747597592250017 2208 60-61 2.0385261091693323 2208 62-63 1.921394458163448 2208 64-65 1.7975329699173628 2208 66-67 2.064034583681522 2208 68-69 1.916294776749826 2208 70-71 1.74057330186767 2208 72-73 1.9470417814783225 2208 74-75 1.856583092743211 2208 76-77 1.875773630204339 2208 78-79 2.0377085987246204 2208 80-81 2.2029785334218346 2208 82-83 2.4114314783668966 2208 84-85 2.269595010655326 2208 86-87 2.1540874559129364 2208 88-89 2.250611116796833 2208 90-91 2.184129132648156 2208 92-93 2.500803494365428 2208 94-95 2.5047221304457175 2208 96-97 2.408810811812959 2208 98-99 2.6126504672017106 2208 100-101 2.5184795017357473 2208 102-103 2.416116176644209 2208 104-105 2.2225351583573882 2208 106-107 2.264321461136255 2208 108-109 1.973685698531952 2208 110-111 2.0522677893339143 2208 112-113 2.277659871678349 2208 114-115 2.3508845819373754 2208 116-117 2.191422891949717 2208 118-119 2.32117585690715 2208 120-121 2.244454624932274 2209 1 0.6230721001628012 2209 2 1.0641515170458398 2209 3 0.9811288159595648 2209 4 1.0961483766445426 2209 5 1.1068431879847296 2209 6 1.1116693878813777 2209 7 1.196587146221539 2209 8 1.0811106873872092 2209 9 1.0716507628762812 2209 10-11 1.2704670587661226 2209 12-13 1.0924655534171706 2209 14-15 1.278842759825416 2209 16-17 1.3910502003321312 2209 18-19 1.3317429658156428 2209 20-21 1.3572156878637287 2209 22-23 1.555324248174621 2209 24-25 1.4828350264465442 2209 26-27 1.39188314439145 2209 28-29 1.3121236807909682 2209 30-31 1.4069741614037454 2209 32-33 1.6091333139940076 2209 34-35 1.5696034895178173 2209 36-37 1.2825965431262318 2209 38-39 1.4152252261610414 2209 40-41 1.6608055286253247 2209 42-43 1.5486130166778551 2209 44-45 1.551561145454663 2209 46-47 1.5703625694830663 2209 48-49 1.7054350585014006 2209 50-51 1.6400021881324136 2209 52-53 1.5644803711957707 2209 54-55 1.4688108770239552 2209 56-57 1.1760562281774867 2209 58-59 1.5484602027674086 2209 60-61 1.7786661271846853 2209 62-63 1.9397832247962938 2209 64-65 1.9490006126053672 2209 66-67 2.0420503490107116 2209 68-69 2.1902401758049628 2209 70-71 1.7177985203132522 2209 72-73 1.8816164254471737 2209 74-75 1.9821223179312533 2209 76-77 1.8330993013140322 2209 78-79 1.5135988191275658 2209 80-81 1.5012475017961577 2209 82-83 1.5137027803748104 2209 84-85 1.7079960155620348 2209 86-87 1.6800339267672229 2209 88-89 1.8571192560090992 2209 90-91 1.9730075062499743 2209 92-93 1.947402622483434 2209 94-95 1.9629466685649 2209 96-97 1.930313306976558 2209 98-99 2.3636491027481945 2209 100-101 2.407942613982005 2209 102-103 2.2579336363282216 2209 104-105 2.1014228056822617 2209 106-107 2.3501841644265227 2209 108-109 2.596506468631034 2209 110-111 2.3291121770211483 2209 112-113 2.395825726894248 2209 114-115 2.7271364395325186 2209 116-117 2.7192241074155525 2209 118-119 2.802055454336351 2209 120-121 2.583727511834663 2210 1 0.7275034557814024 2210 2 0.9443542518957919 2210 3 0.7851429836100969 2210 4 0.7830547538494272 2210 5 0.9149631548563875 2210 6 0.9544441504322592 2210 7 0.7446606805569402 2210 8 0.7812750074633357 2210 9 0.8933018051853949 2210 10-11 0.6607646851560176 2210 12-13 0.5693517100014276 2210 14-15 1.0295123751490323 2210 16-17 1.0040852317864264 2210 18-19 0.9172720059749437 2210 20-21 0.8276160473414365 2210 22-23 0.9451130550258497 2210 24-25 1.143678169056983 2210 26-27 1.0041813787215688 2210 28-29 1.0980461199528904 2210 30-31 1.2596246486370077 2210 32-33 1.237128961494406 2210 34-35 1.2270809340542854 2210 36-37 1.1844736687143467 2210 38-39 1.1028375629423053 2210 40-41 1.2887262849382068 2210 42-43 1.277195238391009 2210 44-45 1.2597509373452667 2210 46-47 1.4361465480553761 2210 48-49 1.35926926703371 2210 50-51 1.2638380707030308 2210 52-53 1.3744362735024183 2210 54-55 1.3245649431748987 2210 56-57 1.3997771307484612 2210 58-59 1.0929841150830484 2210 60-61 1.458221053262676 2210 62-63 1.465025960351106 2210 64-65 1.6446505359520387 2210 66-67 1.6552880272076926 2210 68-69 1.5639207692955956 2210 70-71 1.4273947246516627 2210 72-73 1.4516297737000023 2210 74-75 1.532182671417658 2210 76-77 1.6872608283894017 2210 78-79 1.1283442331436717 2210 80-81 1.3314625716652344 2210 82-83 1.0903935590669462 2210 84-85 1.0338046201189464 2210 86-87 0.7187042117061644 2210 88-89 1.0437767301496095 2210 90-91 1.0964711355650287 2210 92-93 1.2253315287196678 2210 94-95 1.3220025550122365 2210 96-97 1.408496843250326 2210 98-99 1.4082468049103447 2210 100-101 1.4454564908785095 2210 102-103 1.3056194986341616 2210 104-105 1.608584826158392 2210 106-107 1.50202604710287 2210 108-109 1.7368723781624809 2210 110-111 1.3986662660799887 2210 112-113 1.5754236052709594 2210 114-115 1.645133893232142 2210 116-117 1.8043908063882235 2210 118-119 1.868747158154921 2210 120-121 1.7388943429682477 2211 1 0.2032000319325249 2211 2 0.6998359520138564 2211 3 0.5765573873881351 2211 4 0.8380417668364402 2211 5 0.9437045952341947 2211 6 0.7636779166660261 2211 7 0.9134741398249488 2211 8 0.5666032247006427 2211 9 0.7561624899551731 2211 10-11 0.9366418988514127 2211 12-13 0.7071350630120534 2211 14-15 0.9356871095055865 2211 16-17 0.8396472152575001 2211 18-19 0.852241899717562 2211 20-21 0.9306101441537109 2211 22-23 0.28365260638357626 2211 24-25 0.6992785232482461 2211 26-27 1.02013474353857 2211 28-29 1.1005543844157089 2211 30-31 1.2247822637491694 2211 32-33 1.069316682863942 2211 34-35 1.090494747513553 2211 36-37 1.0684984621027809 2211 38-39 0.9231356739222321 2211 40-41 0.8581914561306476 2211 42-43 0.8087707991938444 2211 44-45 1.0628660495058355 2211 46-47 1.080516087606732 2211 48-49 1.1123902823819947 2211 50-51 0.9251066657443516 2211 52-53 1.1006765332426767 2211 54-55 0.9126658876849376 2211 56-57 1.186022113038895 2211 58-59 1.2249079639614422 2211 60-61 1.5682098372060054 2211 62-63 1.5597107301267812 2211 64-65 1.3174952821149688 2211 66-67 1.48223371788065 2211 68-69 1.309476849578953 2211 70-71 1.4751503326799948 2211 72-73 1.5326185576433318 2211 74-75 1.5587363904259242 2211 76-77 1.4815683844696856 2211 78-79 1.3263365589996354 2211 80-81 1.7683775657620444 2211 82-83 1.8247347633172417 2211 84-85 1.5251346083125696 2211 86-87 1.3640808350827882 2211 88-89 1.5105051835144252 2211 90-91 1.52543394548238 2211 92-93 1.5505753303725598 2211 94-95 1.7032451760275897 2211 96-97 1.8729401726482031 2211 98-99 1.9926682925136525 2211 100-101 2.1092475180331753 2211 102-103 1.8084069838761927 2211 104-105 1.8237554282835404 2211 106-107 1.9757108168785464 2211 108-109 2.145551244750436 2211 110-111 2.1761526887482283 2211 112-113 1.9607553644209013 2211 114-115 1.6920447550975481 2211 116-117 1.452793403790821 2211 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104-105 1.5940143712791581 2312 106-107 1.3225827301026456 2312 108-109 1.6556680229641465 2312 110-111 2.106804892763922 2312 112-113 2.0828269083858224 2312 114-115 2.3109129757299023 2312 116-117 2.199364370788281 2312 118-119 2.3530051255510145 2312 120-121 2.1269544305528605 2313 1 -0.31094420072615137 2313 2 -2.144222998664077 2313 3 -0.4606082640168552 2313 4 -1.7403602767870652 2313 5 -1.832032877746748 2313 6 -0.15692573827600853 2313 7 0.21818626401437768 2313 8 0.26147923177472165 2313 9 0.05979463772487037 2313 10-11 -0.16646598088920683 2313 12-13 -0.026541494268357724 2313 14-15 0.11305790926979142 2313 16-17 0.0488136526715266 2313 18-19 -0.08921693305901357 2313 20-21 -5.1454818189038605 2313 22-23 -2.0371601269628528 2313 24-25 -0.23809070449787129 2313 26-27 0.08873368242636559 2313 28-29 0.5093686897802172 2313 30-31 0.40772350957349346 2313 32-33 0.4436424836106525 2313 34-35 0.4675652918248119 2313 36-37 -0.021465774485946554 2313 38-39 0.12882244932015396 2313 40-41 0.3728615288364878 2313 42-43 0.09448822894429298 2313 44-45 -0.00925263785907049 2313 46-47 0.34014037528604746 2313 48-49 0.28121087581882875 2313 50-51 0.3572772297058435 2313 52-53 0.12544446407388676 2313 54-55 0.22985826339725435 2313 56-57 0.2835964891442231 2313 58-59 0.4033469500097837 2313 60-61 0.3772340069839615 2313 62-63 0.3942408932236603 2313 64-65 0.4302199824490245 2313 66-67 0.5493832561003451 2313 68-69 0.38056507909359283 2313 70-71 0.07900413499871917 2313 72-73 0.1610485045877219 2313 74-75 0.14985606030212395 2313 76-77 0.21992719759603574 2313 78-79 -0.23181710576750802 2313 80-81 -0.23950296086400868 2313 82-83 -0.22648237167981122 2313 84-85 0.03638868573445819 2313 86-87 0.4795832913610383 2313 88-89 0.3945829984092981 2313 90-91 0.7112606304892601 2313 92-93 0.576291974113964 2313 94-95 0.4122842799772819 2313 96-97 0.4058047825912183 2313 98-99 0.41346609168834547 2313 100-101 0.3314577754511383 2313 102-103 0.1601004403509414 2313 104-105 0.4903806984741479 2313 106-107 0.647368902946539 2313 108-109 0.43250113667419754 2313 110-111 0.9645645574847173 2313 112-113 0.9958483099897713 2313 114-115 0.9898533618965288 2313 116-117 0.6559445140285867 2313 118-119 0.3466841970445067 2313 120-121 0.17545080732420004 2314 1 -0.5974408225401078 2314 2 -5.446300229561579 2314 3 -1.8079232534663383 2314 4 -0.5040249888377915 2314 5 -2.751953615713738 2314 6 -0.49243957332062394 2314 7 0.08687867921079828 2314 8 0.42879815126939747 2314 9 0.3411344525719926 2314 10-11 0.261400243310689 2314 12-13 0.5183767185527728 2314 14-15 0.4473560013613991 2314 16-17 0.5960797920265293 2314 18-19 0.5891657982489349 2314 20-21 0.6137716928853507 2314 22-23 0.6526032071846473 2314 24-25 0.8154974818597296 2314 26-27 0.6397609117628633 2314 28-29 0.587998977206091 2314 30-31 0.7844725174207312 2314 32-33 0.6478266962150769 2314 34-35 0.8597764564588104 2314 36-37 0.7638949908077137 2314 38-39 0.419584272053072 2314 40-41 0.41666408630421614 2314 42-43 0.7454276750282887 2314 44-45 0.7821023645926104 2314 46-47 0.7461342451501238 2314 48-49 0.7371539672952139 2314 50-51 0.8801881076669247 2314 52-53 0.8582252648848723 2314 54-55 0.6605590786061626 2314 56-57 0.6809433413433013 2314 58-59 0.9105101001430214 2314 60-61 0.9349441496225595 2314 62-63 1.0149630665753833 2314 64-65 1.1837115704994758 2314 66-67 0.9358839181476704 2314 68-69 0.9552552207405007 2314 70-71 1.1878232298762583 2314 72-73 0.8333929234644266 2314 74-75 1.027665629411235 2314 76-77 1.0290022963521928 2314 78-79 0.8626463053280737 2314 80-81 0.9995337740397474 2314 82-83 1.0810712119153756 2314 84-85 1.2754043012364669 2314 86-87 1.144817817726313 2314 88-89 1.012844302339527 2314 90-91 1.1020053740538138 2314 92-93 1.2976407509333079 2314 94-95 1.3858887007271896 2314 96-97 1.239252589203609 2314 98-99 1.322494727760649 2314 100-101 1.5740646074857771 2314 102-103 1.6771493069736572 2314 104-105 1.5962467500458928 2314 106-107 1.6058683602697386 2314 108-109 1.5754684677143906 2314 110-111 1.5360298583076393 2314 112-113 1.7588968864502732 2314 114-115 1.9960073719199762 2314 116-117 1.9056318550591769 2314 118-119 1.8131199582520203 2314 120-121 1.8315654250722098 2315 1 0.05249395639886245 2315 2 0.4354747040664009 2315 3 0.2584687174373954 2315 4 0.24883322824859277 2315 5 0.541950900652914 2315 6 0.4822132204427092 2315 7 0.4937368656049159 2315 8 0.42365412782051237 2315 9 0.1387634751768445 2315 10-11 0.2910819645328573 2315 12-13 0.2604519759841395 2315 14-15 -0.3702406409049175 2315 16-17 0.04348629571727258 2315 18-19 0.31274436277174544 2315 20-21 0.3693753329878646 2315 22-23 0.38078561130968325 2315 24-25 0.3819024969756697 2315 26-27 0.3142234134893158 2315 28-29 0.36834748786397853 2315 30-31 0.3318068942910344 2315 32-33 0.1282066664435817 2315 34-35 0.2112205274807124 2315 36-37 0.09925708279243395 2315 38-39 0.22287294814226755 2315 40-41 -0.17023875733404736 2315 42-43 -0.20970867535459803 2315 44-45 -0.13831950057626585 2315 46-47 -0.014938756399835995 2315 48-49 -0.0296983875687431 2315 50-51 -0.061927817014264974 2315 52-53 -0.21128734196258137 2315 54-55 -0.0797774620687548 2315 56-57 -0.02015851093483434 2315 58-59 -0.2603038586986557 2315 60-61 -0.020223660330941584 2315 62-63 -0.10491324360064169 2315 64-65 -0.4546951940755086 2315 66-67 -0.1920782689830567 2315 68-69 -0.399459111012181 2315 70-71 -0.4772765967124535 2315 72-73 -0.19606781345683189 2315 74-75 0.30257547088569225 2315 76-77 0.10209055195736738 2315 78-79 -0.38797214379182776 2315 80-81 0.040095135548579464 2315 82-83 0.049178113070937 2315 84-85 -0.08441875786148856 2315 86-87 -0.07852671828338487 2315 88-89 -0.14650302666619552 2315 90-91 -0.21737065221876506 2315 92-93 0.038805215430031126 2315 94-95 -0.25151672873431963 2315 96-97 -0.174780681210585 2315 98-99 -1.8214696385208278 2315 100-101 -10.489080889191783 2315 102-103 -4.348230126140226 2315 104-105 -1.492195310632713 2315 106-107 -0.33256832926266355 2315 108-109 -0.14274432175202634 2315 110-111 0.14063216329666872 2315 112-113 0.42245979791843524 2315 114-115 0.29785427890707084 2315 116-117 0.5529017781750589 2315 118-119 0.9114456952500127 2315 120-121 1.1686048492853374 2316 1 -1.352530021678323 2316 2 -3.191373130294565 2316 3 -1.2579124959294283 2316 4 -3.801482673403232 2316 5 -4.508768441662685 2316 6 -1.8994340353995725 2316 7 -7.712505046551591 2316 8 -3.4860817327260385 2316 9 -1.8517290269164732 2316 10-11 -0.9544725760776345 2316 12-13 -0.6336943283471399 2316 14-15 -4.522067542023898 2316 16-17 -3.8006485904820124 2316 18-19 -4.775076296435074 2316 20-21 -1.3314201302076896 2316 22-23 -3.5587706039601628 2316 24-25 -0.9638271216587242 2316 26-27 -3.5646492362785267 2316 28-29 -2.269728176290691 2316 30-31 -0.8266425233842298 2316 32-33 -0.3195934401256437 2316 34-35 -0.30254593365958016 2316 36-37 -0.1747818910981067 2316 38-39 -0.17309264521999523 2316 40-41 -0.2789469860011664 2316 42-43 -0.524586501342263 2316 44-45 -0.6438725187691645 2316 46-47 -0.21519756739169082 2316 48-49 -0.11683015281194287 2316 50-51 -9.816344763891038E-4 2316 52-53 -0.1859314768487721 2316 54-55 -0.457930769520992 2316 56-57 -0.2503594700579228 2316 58-59 -0.405409286117397 2316 60-61 -0.011800884963655278 2316 62-63 -0.04980172651150383 2316 64-65 -0.038580403788536444 2316 66-67 -0.3507256009462161 2316 68-69 -0.01160419740937968 2316 70-71 0.3582528240076499 2316 72-73 0.3737160032440876 2316 74-75 0.38592402839501716 2316 76-77 0.26766488399664823 2316 78-79 -0.004478956610579843 2316 80-81 -0.10374668526286968 2316 82-83 0.09797159712361392 2316 84-85 0.0208640312075552 2316 86-87 -0.013485232403809277 2316 88-89 -0.18607066006804018 2316 90-91 -0.041245966217395846 2316 92-93 0.23422654450059355 2316 94-95 0.14748989378223953 2316 96-97 0.14645704429752726 2316 98-99 0.280482862050075 2316 100-101 0.12633739504358132 2316 102-103 0.30796611348830183 2316 104-105 0.5293164500432397 2316 106-107 0.8472976979886084 2316 108-109 0.06052507004214647 2316 110-111 0.01202213056469148 2316 112-113 0.04261660693114777 2316 114-115 0.01750107802407186 2316 116-117 0.5925455324568567 2316 118-119 0.355188558917515 2316 120-121 -0.0625362027052212 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11696.0 3 1893.0 4 1650.0 5 1783.0 6 2030.0 7 2181.0 8 2646.0 9 2854.0 10 3351.0 11 3871.0 12 4315.0 13 5214.0 14 6470.0 15 7012.0 16 5862.0 17 6136.0 18 6441.0 19 6771.0 20 7411.0 21 8137.0 22 8862.0 23 9706.0 24 10889.0 25 12169.0 26 13617.0 27 15628.0 28 18311.0 29 20783.0 30 24237.0 31 28967.0 32 35235.0 33 43839.0 34 55471.0 35 71037.0 36 91676.0 37 97281.0 38 36845.0 39 152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.54964223767233 17.43494111617213 14.776843757329711 21.23857288882583 2 12.981814941576719 20.547845153110043 36.67001181758556 29.800328087727678 3 24.851646988426566 22.38094543119427 32.30826486084152 20.459142719537645 4 10.19303847343751 13.907292499693543 45.83306579185468 30.066603235014274 5 10.475316271895325 37.82289357591182 38.45872084453973 13.24306930765313 6 25.757580180032924 34.73149758905325 19.67986363264877 19.831058598265052 7 21.35772025563954 34.15589342516123 24.736189336679605 19.750196982519626 8 20.006741533684323 26.832179587453158 23.305569499083617 29.8555093797789 9 18.51014154384941 20.690938275207937 30.435429738800522 30.363490442142126 10-11 23.75236845887655 21.224091048046244 28.965349182156647 26.05819131092056 12-13 28.6130467677837 21.222465988094466 27.59708541298373 22.567401831138103 14-15 23.89465950602674 22.569513096427148 32.9289726485637 20.60685474898241 16-17 18.968497473595885 27.686616442604112 32.18036883675964 21.16451724704037 18-19 16.63172504580843 32.399894627844375 30.640675841244153 20.327704485103048 20-21 19.192103460026573 26.977290953231233 26.220882165579535 27.609723421162663 22-23 17.023620771803046 26.26894247558063 29.804652357370976 26.902784395245344 24-25 23.2997786206182 24.491316295244502 27.8943858363894 24.3145192477479 26-27 21.500797947447115 21.821874195029007 34.79109246922958 21.88623538829429 28-29 18.08966673818521 28.856272541112215 32.06555769138143 20.988503029321144 30-31 18.189288758626326 26.327155268043768 29.86467905977417 25.618876913555738 32-33 19.635375654881056 25.240259887162324 31.59250580352875 23.531858654427868 34-35 23.841323501077227 23.688643277107307 29.061863224209304 23.40816999760616 36-37 21.557788687997792 22.3234584245931 34.62737917036754 21.49137371704157 38-39 21.756331885377985 25.324841191994206 31.971204371890984 20.94762255073682 40-41 19.348279689882197 28.509351629592704 32.15169586093597 19.990672819589122 42-43 18.112116999266565 28.52681780531503 27.16284806223614 26.19821713318227 44-45 18.64914999941621 25.652533071794693 27.50687414620477 28.191442782584332 46-47 22.564346955397205 26.82721415705999 28.903948831873233 21.704490055669567 48-49 21.96635269030332 24.84716549183995 29.598116579688867 23.588365238167867 50-51 16.66397796677351 26.525642532361122 35.248928595673796 21.561450905191577 52-53 19.077274064170147 29.03026636238041 28.466042573315885 23.426417000133558 54-55 17.042460548575868 25.16833726716325 31.82380821010009 25.965393974160794 56-57 21.53999408873466 23.09786793065575 28.882369468679414 26.479768511930175 58-59 24.59878883227173 23.638348439485032 28.474116926795062 23.288745801448176 60-61 22.314976299650098 23.469515137489772 33.92862226978758 20.28688629307255 62-63 19.82426059301466 25.922730428041806 31.986017344740773 22.26699163420276 64-65 17.8602190409731 30.44529377242747 30.44770258078267 21.246784605816764 66-67 18.946668447718466 26.510518059792243 26.865122553152354 27.677690939336934 68-69 17.32604325872466 26.856494720753943 29.618811136851665 26.19865088366974 70-71 22.348613212893877 25.07916985585828 26.93900415422639 25.63321277702146 72-73 20.27005214210641 22.647013496764036 33.26540763090488 23.81752673022467 74-75 18.311992812639236 28.4121337842477 30.759712797738608 22.516160605374452 76-77 18.561982360254618 25.522698352172533 29.31062694582871 26.604692341744133 78-79 19.8406699777049 24.506283596923815 31.282227670026636 24.370818755344654 80-81 23.157531301803182 24.074194379580852 28.49473236255711 24.27354195605886 82-83 19.985030869268375 22.76994636669989 33.508946793867764 23.736075970163967 84-85 20.731747379394474 24.815358547893624 31.39280648436981 23.060087588342093 86-87 19.905897535467258 27.601467449540003 30.93957938563324 21.553055629359502 88-89 18.720445915386446 26.25264061503154 27.262242453352176 27.764671016229837 90-91 18.06312725021681 23.62264101358671 29.287785392461174 29.02644634373531 92-93 21.510433623525255 25.67081636734998 30.3368571966672 22.48189281245757 94-95 20.267797379690816 23.980376513422353 30.43034589278324 25.321480214103598 96-97 15.314453150663333 25.410430819138885 34.54480685479691 24.73030917540087 98-99 18.57421251808027 27.708060897190173 28.680908916337554 25.036817668392004 100-101 16.406775305368395 23.662464162880664 32.21263320968447 27.718127322066465 102-103 20.28485777913705 22.700813201588012 30.0113794208393 27.002949598435638 104-105 22.968363188378717 23.55274066981176 28.696459432948764 24.782436708860757 106-107 21.024589655426606 23.62959984059895 32.72523974019965 22.620570763774797 108-109 19.635603005209635 24.850564243143474 31.28137421049021 24.23245854115669 110-111 18.367850422540556 27.721040023473662 29.415727647100898 24.495381906884877 112-113 20.135541509130984 24.449075222982934 26.157467556165425 29.257915711720656 114-115 18.65951073389915 24.123317947056027 29.423262766668714 27.793908552376113 116-117 22.439691417466147 22.53051110898582 27.52340395708113 27.506393516466908 118-119 20.51265391699451 21.82370878360769 32.55385954141185 25.109777757985945 120-121 18.353295314451383 27.4741774031201 29.660056305706263 24.512470976722256 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1993.0 1 2262.5 2 1466.0 3 385.0 4 371.5 5 374.0 6 352.5 7 318.0 8 321.0 9 331.5 10 342.0 11 366.5 12 387.5 13 412.0 14 448.0 15 481.5 16 509.0 17 553.5 18 620.5 19 678.0 20 761.5 21 883.5 22 1003.0 23 1247.0 24 1509.0 25 1812.5 26 2359.5 27 3166.0 28 4424.5 29 5916.5 30 7584.0 31 9595.0 32 11498.5 33 12945.5 34 14262.0 35 15399.5 36 16157.5 37 16756.5 38 17190.0 39 17312.0 40 17301.5 41 17169.0 42 17177.0 43 17866.5 44 18450.5 45 19474.5 46 24549.5 47 47213.5 48 95697.5 49 95940.0 50 48339.0 51 24144.0 52 15594.5 53 12065.5 54 9885.0 55 8323.5 56 6869.5 57 5363.0 58 4159.5 59 3285.0 60 2630.5 61 2053.0 62 1523.0 63 1093.0 64 769.5 65 541.5 66 360.0 67 243.0 68 162.0 69 118.0 70 85.5 71 48.0 72 33.0 73 23.5 74 21.0 75 18.5 76 11.5 77 7.5 78 6.5 79 6.5 80 5.5 81 4.0 82 5.5 83 4.0 84 0.5 85 0.0 86 1.5 87 3.5 88 2.0 89 1.5 90 2.0 91 2.5 92 2.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 1.5 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.252300235836454 2 1.134556755999532 3 1.0454501472353122 4 1.0364961606171896 5 1.0369294180341957 6 1.0428506027332767 7 1.0237872763850158 8 1.0289863653890867 9 1.0295640419450949 10-11 1.028336479263578 12-13 1.027903221846572 14-15 1.0266034495955543 16-17 1.0349797596576689 18-19 1.0453779376658112 20-21 1.0423451357467697 22-23 1.034546502240663 24-25 1.0369294180341957 26-27 1.0446558419708012 28-29 1.053104361602417 30-31 1.0567148400774664 32-33 1.052165637198904 34-35 1.059603222857506 36-37 1.0603975281220168 38-39 1.0578701931894823 40-41 1.0592421750100012 42-43 1.0537542477279258 44-45 1.04747201518134 46-47 1.0564260017994624 48-49 1.053176571171918 50-51 1.077872243941256 52-53 1.0583756601759893 54-55 1.0609029951085238 56-57 1.0537542477279258 58-59 1.0430672314417795 60-61 1.0865373922813746 62-63 1.0741173463272047 64-65 1.0749116515917156 66-67 1.0745506037442105 68-69 1.066968598946607 70-71 1.1300797626904708 72-73 1.134773384708035 74-75 1.1400446832816074 76-77 1.148420993343722 78-79 1.1191761176958215 80-81 1.0751282803002185 82-83 1.110077711938697 84-85 1.0861041348643687 86-87 1.0731786219236918 88-89 1.0767891003987413 90-91 1.0824214468198183 92-93 1.0802551597347887 94-95 1.0735396697711967 96-97 1.0799663214567847 98-99 1.1027123358495963 100-101 1.0917364812854458 102-103 1.071662220964171 104-105 1.060180899413514 106-107 1.0635747491800602 108-109 1.0617695099425357 110-111 1.0695681434486424 112-113 1.0685572094756286 114-115 1.0691348860316365 116-117 1.091158804729438 118-119 1.0863929731423727 120-121 1.071373382686167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 692429.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.15276474369709 #Duplication Level Percentage of deduplicated Percentage of total 1 91.05610587782978 50.219959859571205 2 5.318481403592988 5.866579072921839 3 1.3074130132649198 2.163223270303447 4 0.6079424916774506 1.3411883688473385 5 0.3843307140673034 1.059845072836555 6 0.24178597891512527 0.8001099128058246 7 0.175165643719266 0.6762608677458857 8 0.1360034790079914 0.6000774305641677 9 0.10747061748516125 0.5334571514717062 >10 0.580694423628849 5.704126355920417 >50 0.0439953107372619 1.6264480344500645 >100 0.026553376279117524 3.0167101793918416 >500 0.004685889931608128 1.7021131396262452 >1k 0.007809816552680213 10.756694585209504 >5k 2.6032721842267375E-4 1.1012406394713876 >10k+ 0.001301636092113369 12.831966058862623 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 27530 3.9758588967244295 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 17867 2.580336756548325 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 16892 2.439528096021397 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16169 2.335113058522968 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10915 1.57633490220658 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7670 1.1076947961451644 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4538 0.655374052790972 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4466 0.6449758747828297 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4408 0.6365995647207151 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4167 0.6017945522212386 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3957 0.5714665330308234 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3672 0.5303070784152599 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3667 0.52958498272025 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3607 0.5209198343801313 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3368 0.4864036601586588 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3190 0.46069705341630696 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3115 0.44986561799115865 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2475 0.3574373690298933 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2457 0.3548378245278577 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2287 0.3302865708975216 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2156 0.31136766368826263 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2141 0.309201376603233 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2056 0.2969257497880649 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1748 0.252444654975456 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1685 0.2433462492183314 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1651 0.2384359984922642 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1590 0.2296264310131436 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1551 0.2239940845920665 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1550 0.2238496654530645 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1545 0.22312756975805462 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1502 0.2169175467809696 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1381 0.1994428309617304 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1376 0.1987207352667205 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1246 0.17994624719646346 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1212 0.17503599647039625 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1155 0.16680410554728353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 836 0.12073440020565285 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 812 0.11726834086960541 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 805 0.11625740689659156 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 784 0.11322460497755005 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 740 0.10687016286146304 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 711 0.10268200783040571 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 706 0.10195991213539583 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.444191390019771E-4 0.0 5 0.0 0.0 0.0 1.444191390019771E-4 0.0 6 0.0 0.0 0.0 1.444191390019771E-4 0.0 7 0.0 0.0 0.0 1.444191390019771E-4 0.0 8 0.0 0.0 0.0 1.444191390019771E-4 0.0 9 0.0 0.0 0.0 2.888382780039542E-4 0.0 10-11 0.0 0.0 0.0 4.332574170059313E-4 0.0 12-13 0.0 0.0 0.0 0.0011553531120158168 0.0 14-15 0.0 0.0 0.0 0.0014441913900197709 0.0 16-17 0.0 0.0 0.0 0.0018052392375247138 0.0 18-19 0.0 0.0 0.0 0.002743963641037565 0.0 20-21 0.0 0.0 0.0 0.004765831587065244 0.0 22-23 0.0 0.0 0.0 0.007654214367104785 0.0 24-25 0.0 0.0 0.0 0.01090364499464927 0.0 26-27 0.0 0.0 0.0 0.013792027774688811 0.0 28-29 0.0 0.0 0.0 0.02043530816877976 0.0 30-31 0.0 0.0 0.0 0.03328861153995572 0.0 32-33 0.0 0.0 0.0 0.053146243152727576 0.0 34-35 0.0 0.0 0.0 0.07220956950098854 0.0 36-37 0.0 0.0 0.0 0.0890343991947189 0.0 38-39 1.444191390019771E-4 0.0 0.0 0.11524647292357773 0.0 40-41 1.444191390019771E-4 0.0 0.0 0.19648223861218983 0.0 42-43 1.444191390019771E-4 0.0 0.0 0.25172255928044607 0.0 44-45 1.444191390019771E-4 0.0 0.0 0.3165667526923338 0.0 46-47 1.444191390019771E-4 0.0 0.0 0.5036617472693952 0.0 48-49 1.444191390019771E-4 0.0 0.0 0.5608517263141781 0.0 50-51 1.444191390019771E-4 1.444191390019771E-4 0.0 0.6350109541916933 0.0 52-53 1.444191390019771E-4 1.444191390019771E-4 0.0 0.7070761045536799 0.0 54-55 1.444191390019771E-4 1.444191390019771E-4 0.0 0.8031870415594956 0.0 56-57 1.444191390019771E-4 1.444191390019771E-4 0.0 1.3298114319302052 0.0 58-59 1.444191390019771E-4 1.444191390019771E-4 0.0 1.6014638323929242 0.0 60-61 1.444191390019771E-4 1.444191390019771E-4 0.0 1.7259531302126283 0.0 62-63 1.444191390019771E-4 1.444191390019771E-4 0.0 1.9459034789126393 0.0 64-65 1.444191390019771E-4 1.444191390019771E-4 0.0 2.1346592935882236 0.0 66-67 1.444191390019771E-4 1.444191390019771E-4 0.0 2.705981407480045 0.0 68-69 1.444191390019771E-4 1.444191390019771E-4 0.0 3.292972997953581 0.0 70-71 1.444191390019771E-4 1.444191390019771E-4 0.0 3.4357313168570354 0.0 72-73 1.444191390019771E-4 2.1662870850296565E-4 0.0 3.575529043410949 0.0 74-75 1.444191390019771E-4 2.888382780039542E-4 0.0 4.113634755332315 0.0 76-77 1.444191390019771E-4 2.888382780039542E-4 0.0 4.592528620262872 0.0 78-79 1.444191390019771E-4 2.888382780039542E-4 0.0 4.991414282186332 0.0 80-81 1.444191390019771E-4 2.888382780039542E-4 0.0 5.286318164028369 0.0 82-83 1.444191390019771E-4 2.888382780039542E-4 0.0 5.843776040576001 0.0 84-85 1.444191390019771E-4 2.888382780039542E-4 0.0 6.908145095020572 0.0 86-87 1.444191390019771E-4 2.888382780039542E-4 0.0 8.083716886496667 0.0 88-89 1.444191390019771E-4 2.888382780039542E-4 0.0 9.121007352378367 0.0 90-91 1.444191390019771E-4 2.888382780039542E-4 0.0 10.324163199403838 0.0 92-93 1.444191390019771E-4 2.888382780039542E-4 0.0 11.549415174696612 0.0 94-95 1.444191390019771E-4 2.888382780039542E-4 0.0 12.66108149716433 0.0 96-97 1.444191390019771E-4 2.888382780039542E-4 0.0 13.64197628926576 0.0 98-99 1.444191390019771E-4 2.888382780039542E-4 0.0 14.31626924926599 0.0 100-101 1.444191390019771E-4 2.888382780039542E-4 0.0 14.639190444074412 0.0 102-103 1.444191390019771E-4 2.888382780039542E-4 0.0 14.978503211159556 0.0 104-105 1.444191390019771E-4 2.888382780039542E-4 0.0 15.424758350675665 0.0 106-107 1.444191390019771E-4 2.888382780039542E-4 0.0 15.964308253987053 0.0 108-109 1.444191390019771E-4 2.888382780039542E-4 0.0 16.747276616086268 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 105 1.5643309E-10 49.32314 2 TACCTGG 505 0.0 46.718613 2 ACCTGGG 605 0.0 37.02656 3 GTACCCG 90 2.8930552E-4 32.00146 1 GTATAAC 180 8.5128704E-10 32.00146 1 TAACGCA 165 1.3287718E-8 31.325598 4 ATAACGC 155 2.6815542E-7 29.64575 3 GGGCGTC 110 9.523403E-4 26.10657 7 GTGTTAT 295 9.094947E-12 25.38421 1 GTACCTG 1060 0.0 24.997372 1 CCTGGGG 445 0.0 24.520786 4 CGCCGTA 425 0.0 24.342058 114-115 TCTCGGG 380 0.0 24.199709 114-115 TATGTAC 390 0.0 23.561132 4 GCCGTAT 340 0.0 22.820683 114-115 GGCGCCG 155 5.2023097E-10 22.249744 112-113 GTACGCA 285 4.800313E-9 22.1693 3 TACCCGG 130 0.0024911307 22.132177 2 AGTACGC 260 4.3330147E-8 22.132177 2 TACATGG 1850 0.0 22.084322 2 >>END_MODULE