##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003837_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2998495 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74115814767075 31.0 31.0 34.0 30.0 34.0 2 31.913498605133576 31.0 31.0 34.0 30.0 34.0 3 31.98170849042603 33.0 31.0 34.0 30.0 34.0 4 35.675889738018576 37.0 35.0 37.0 33.0 37.0 5 35.55023937008399 37.0 35.0 37.0 33.0 37.0 6 35.643398104715864 37.0 35.0 37.0 33.0 37.0 7 35.604474244579364 37.0 35.0 37.0 33.0 37.0 8 35.617420072402986 37.0 35.0 37.0 33.0 37.0 9 37.198107383870905 39.0 37.0 39.0 33.0 39.0 10-11 37.09587009483091 39.0 37.0 39.0 33.0 39.0 12-13 37.07496377349304 39.0 37.0 39.0 33.0 39.0 14-15 38.117517454589716 40.0 37.0 41.0 33.0 41.0 16-17 38.188766364459504 40.0 37.5 41.0 33.0 41.0 18-19 38.25549117140432 40.0 38.0 41.0 33.0 41.0 20-21 38.17923224817784 40.0 37.5 41.0 33.0 41.0 22-23 38.08800231449444 40.0 37.0 41.0 33.0 41.0 24-25 37.92634788452207 40.0 37.0 41.0 32.5 41.0 26-27 37.70210055377781 39.5 37.0 41.0 32.5 41.0 28-29 37.599292978644286 39.0 37.0 41.0 32.0 41.0 30-31 37.463205708196945 39.0 36.5 41.0 32.0 41.0 32-33 37.21348459810672 39.0 36.0 41.0 31.0 41.0 34-35 37.13752799320993 39.0 36.0 41.0 31.0 41.0 36-37 36.96949869851376 39.0 35.5 40.5 30.5 41.0 38-39 36.74671426832461 39.0 35.0 40.0 30.0 41.0 40-41 36.90421177957609 39.0 36.0 41.0 30.5 41.0 42-43 36.794434041077274 39.0 36.0 41.0 30.0 41.0 44-45 36.56744816983186 39.0 35.0 41.0 30.0 41.0 46-47 36.43321249493496 39.0 35.0 41.0 30.0 41.0 48-49 36.1773021132268 39.0 35.0 41.0 29.0 41.0 50-51 35.92112409725546 39.0 35.0 40.0 28.0 41.0 52-53 35.82000737036414 39.0 35.0 40.0 28.0 41.0 54-55 35.66267294092536 39.0 35.0 40.0 28.0 41.0 56-57 35.30542772290766 38.0 34.0 40.0 27.5 41.0 58-59 35.01820196465226 38.0 34.0 40.0 26.0 41.0 60-61 34.50737253188683 38.0 33.5 40.0 26.0 41.0 62-63 34.35345148149322 37.0 33.0 40.0 26.0 41.0 64-65 33.931553829504466 37.0 33.0 39.5 25.5 41.0 66-67 33.47156473497538 36.0 32.0 39.0 23.5 40.5 68-69 33.076572413827606 36.0 32.0 39.0 23.0 40.0 70-71 32.52364002607975 35.0 31.5 37.5 22.5 40.0 72-73 32.06382601938639 35.0 31.0 37.0 21.5 39.5 74-75 31.73557951572372 35.0 31.0 37.0 20.0 39.0 76-77 30.743660236218503 34.0 29.5 36.0 20.0 38.5 78-79 31.177174215731558 34.0 31.0 36.0 20.0 38.0 80-81 30.88483856067794 34.0 31.0 36.0 20.0 37.0 82-83 30.53473525885486 34.0 30.5 35.0 19.0 37.0 84-85 30.183130370402484 34.0 30.0 35.0 18.0 36.5 86-87 30.051705272144858 34.0 30.0 35.0 15.5 36.0 88-89 29.719193795554105 34.0 30.0 35.0 9.0 36.0 90-91 29.338614204792737 34.0 29.0 35.0 6.5 35.5 92-93 29.109600316158605 34.0 29.0 35.0 2.0 35.0 94-95 28.726250002084377 34.0 29.0 35.0 2.0 35.0 96-97 28.32084045496157 34.0 29.0 35.0 2.0 35.0 98-99 28.053630071085664 34.0 29.0 35.0 2.0 35.0 100-101 27.070314941328903 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.11918770497358722 1101 2 0.14723609581264796 1101 3 0.17720557814025284 1101 4 0.12160606459642054 1101 5 0.0779433602146753 1101 6 0.16966604521619644 1101 7 0.14609518112789743 1101 8 0.05564198911000773 1101 9 0.23099187493700413 1101 10-11 0.2647238710186173 1101 12-13 0.308704657673907 1101 14-15 0.39547894542021567 1101 16-17 0.3911727482879144 1101 18-19 0.2876963469451894 1101 20-21 0.18300113640587057 1101 22-23 0.11431224022956599 1101 24-25 0.22847147758214703 1101 26-27 0.2202257618257093 1101 28-29 0.33613414967519617 1101 30-31 0.311049074949338 1101 32-33 0.4076068994160451 1101 34-35 0.3940947449654928 1101 36-37 0.5079065843768689 1101 38-39 0.25109899221447307 1101 40-41 0.4159267544943859 1101 42-43 0.4352636160332324 1101 44-45 0.44529006682429895 1101 46-47 0.5582900547502945 1101 48-49 0.6435684810704174 1101 50-51 0.5265225904356257 1101 52-53 0.4172840394293402 1101 54-55 0.43657604312638654 1101 56-57 0.48433561007609427 1101 58-59 0.5245967061140178 1101 60-61 0.2980111863461943 1101 62-63 0.26691728093919664 1101 64-65 0.22147904794461937 1101 66-67 0.2071641784175 1101 68-69 0.3165939398544353 1101 70-71 0.3456769645674669 1101 72-73 0.3302457860844683 1101 74-75 0.09990158652328418 1101 76-77 0.16616667286195153 1101 78-79 0.2326271882786557 1101 80-81 0.17208524017399895 1101 82-83 0.3098655110197761 1101 84-85 0.31552684549589216 1101 86-87 0.12661031815788704 1101 88-89 0.2057618768512377 1101 90-91 0.2933307978094426 1101 92-93 0.42309007012567434 1101 94-95 0.49571294963509516 1101 96-97 0.20273137422800858 1101 98-99 0.1353454781289507 1101 100-101 0.31429353348400113 1102 1 0.13820057151910703 1102 2 0.12714046147393177 1102 3 0.13361510065062276 1102 4 0.12251247156121536 1102 5 0.12377415612817089 1102 6 -0.015303822415745572 1102 7 0.11106977445054866 1102 8 0.12235692765167272 1102 9 0.1693218548162747 1102 10-11 0.20580589585528486 1102 12-13 0.10349105158215366 1102 14-15 0.19301513105704515 1102 16-17 0.06876024664367009 1102 18-19 0.13820977862484796 1102 20-21 0.22911736160208562 1102 22-23 0.15605805409580853 1102 24-25 0.1719595854058511 1102 26-27 0.20184191150830344 1102 28-29 0.18123191544658113 1102 30-31 0.20595519447521582 1102 32-33 0.13237420786723675 1102 34-35 0.20161316054370815 1102 36-37 0.11682139284023663 1102 38-39 0.027909248424855093 1102 40-41 0.20348686089550228 1102 42-43 0.3380076224445787 1102 44-45 0.46710436666693766 1102 46-47 0.272241012311639 1102 48-49 0.31044613927143416 1102 50-51 0.5413934134365874 1102 52-53 0.6103624249969855 1102 54-55 0.517216483833586 1102 56-57 0.5504201317338797 1102 58-59 0.46531523033440436 1102 60-61 0.390726802074461 1102 62-63 0.22225201330432753 1102 64-65 0.24855140947457244 1102 66-67 0.20315318753272038 1102 68-69 0.3437281129316361 1102 70-71 0.4066285091862696 1102 72-73 0.42330364900045936 1102 74-75 0.4330199090492357 1102 76-77 0.2696594211190515 1102 78-79 0.43338068017433784 1102 80-81 0.4207406715516093 1102 82-83 0.3339292639976925 1102 84-85 0.3047532450554762 1102 86-87 0.35031160578681053 1102 88-89 0.21339088333566636 1102 90-91 0.3333156220088753 1102 92-93 0.467146478795609 1102 94-95 0.5918384313388785 1102 96-97 0.7053660076946677 1102 98-99 0.6426269392930273 1102 100-101 0.9074664937638381 1103 1 0.008713462045204068 1103 2 0.062322686299797425 1103 3 0.04949517134620862 1103 4 0.09727082073982274 1103 5 0.07408406989345906 1103 6 0.06537900560631726 1103 7 0.08046042217900151 1103 8 0.1196173495414854 1103 9 0.13516630466659763 1103 10-11 0.14321218945048741 1103 12-13 0.04251585957710802 1103 14-15 0.03295950296348593 1103 16-17 0.11556622349880996 1103 18-19 0.07270633320789699 1103 20-21 0.06176622446555058 1103 22-23 0.0780694403548523 1103 24-25 0.11971143843448573 1103 26-27 0.015436476785062325 1103 28-29 0.04705284560986911 1103 30-31 0.11924747902494914 1103 32-33 0.2497579871023916 1103 34-35 0.23176173794394828 1103 36-37 0.20186094811336375 1103 38-39 0.28516646816203206 1103 40-41 0.20484157004572268 1103 42-43 0.4326152842774036 1103 44-45 0.5938655506194337 1103 46-47 0.44782068881975334 1103 48-49 0.3039136237695459 1103 50-51 0.411823891972233 1103 52-53 0.4983901596105227 1103 54-55 0.490088231414596 1103 56-57 0.38689922837931334 1103 58-59 0.3813951252839374 1103 60-61 0.380391544139826 1103 62-63 0.33954394785133957 1103 64-65 0.39643476175604775 1103 66-67 0.4206344586632085 1103 68-69 0.21320384111108126 1103 70-71 0.3478611890156884 1103 72-73 0.4012532287489492 1103 74-75 0.14837882319679352 1103 76-77 0.2275415570789754 1103 78-79 0.39824369506310475 1103 80-81 0.34862353978675387 1103 82-83 0.3529959730287544 1103 84-85 0.44044657315945557 1103 86-87 0.5583445407955203 1103 88-89 0.3509441284319017 1103 90-91 0.3905756963782494 1103 92-93 0.47761460124195665 1103 94-95 0.49310942529698565 1103 96-97 0.48841389849381756 1103 98-99 0.42885619356380644 1103 100-101 0.5831359670717866 1104 1 0.05595332760996996 1104 2 0.001376406391344176 1104 3 0.09540057673834212 1104 4 0.03494724734684951 1104 5 0.040332279240104185 1104 6 0.04241323199988756 1104 7 -0.007681451568522846 1104 8 -0.028674889995158992 1104 9 0.0760973265884175 1104 10-11 0.17000123502120346 1104 12-13 0.16095064199370057 1104 14-15 0.13631714368994352 1104 16-17 0.147830139538641 1104 18-19 0.04230272067828622 1104 20-21 -0.017597397705849005 1104 22-23 -0.07808559610623433 1104 24-25 0.14744656017928293 1104 26-27 0.10767187737594242 1104 28-29 0.05313450087559346 1104 30-31 0.01603797528262163 1104 32-33 0.13712478654823457 1104 34-35 0.06529602373959165 1104 36-37 0.15695242975812107 1104 38-39 0.2601702237475436 1104 40-41 0.3287786534334671 1104 42-43 0.13125716146141286 1104 44-45 0.3597590412324365 1104 46-47 0.3528091005065477 1104 48-49 0.2405816879394962 1104 50-51 0.40923689493840953 1104 52-53 0.3042215406939519 1104 54-55 0.3721156560255068 1104 56-57 0.3620011078412233 1104 58-59 0.33208668076400016 1104 60-61 0.278409724119264 1104 62-63 0.09472984687959496 1104 64-65 0.12744622684677154 1104 66-67 0.3369368785366831 1104 68-69 0.269797691965735 1104 70-71 0.1827000498508724 1104 72-73 0.17194865272755777 1104 74-75 0.1793472463319894 1104 76-77 0.18674718446794714 1104 78-79 0.18551346294337634 1104 80-81 0.23632582593193163 1104 82-83 0.3706107736685311 1104 84-85 0.2784320869088255 1104 86-87 0.22890237043037942 1104 88-89 0.12539949489359614 1104 90-91 0.13910733608161152 1104 92-93 0.05067270579549543 1104 94-95 0.2587388589743256 1104 96-97 0.12316780454821341 1104 98-99 0.29803123134057685 1104 100-101 0.4732188771449053 1105 1 -0.030701309367891838 1105 2 -0.015704097142620554 1105 3 -0.032555261845811145 1105 4 -0.02756528357898702 1105 5 -0.022585377734685608 1105 6 0.11295869468546726 1105 7 -0.012217890040943757 1105 8 0.06353885499490985 1105 9 -0.0029604061850889707 1105 10-11 0.05040449724700835 1105 12-13 0.11615185216481905 1105 14-15 0.08076402132386562 1105 16-17 0.09521631006824549 1105 18-19 0.022362124566328134 1105 20-21 -0.007598935481603064 1105 22-23 0.047576961390028316 1105 24-25 0.0655673008810922 1105 26-27 0.06188253610846317 1105 28-29 0.035838538412896526 1105 30-31 0.08550042532924351 1105 32-33 0.16488163889646046 1105 34-35 0.05099665578489265 1105 36-37 0.053443940846648275 1105 38-39 0.10018363548797282 1105 40-41 0.18566100288229848 1105 42-43 0.2461321733840407 1105 44-45 0.2961082642663726 1105 46-47 0.13124055463897832 1105 48-49 0.18264744634518593 1105 50-51 0.19710644775711472 1105 52-53 0.35606903713662774 1105 54-55 0.221989689805973 1105 56-57 0.29447949138585017 1105 58-59 0.37432867508395873 1105 60-61 0.3770492792154343 1105 62-63 0.17459783175100085 1105 64-65 0.08013869700126719 1105 66-67 0.14178863403423492 1105 68-69 0.03404279979496039 1105 70-71 0.04818494071745505 1105 72-73 0.04836245747674894 1105 74-75 0.2709058550537087 1105 76-77 0.27574425296125327 1105 78-79 0.31883837744109655 1105 80-81 0.2804097446906546 1105 82-83 0.3784750853970884 1105 84-85 0.23217423500636514 1105 86-87 0.29747354316931407 1105 88-89 0.4195115169908483 1105 90-91 0.34142996143466675 1105 92-93 0.48672982443197554 1105 94-95 0.3499996453033063 1105 96-97 0.6265573591577436 1105 98-99 0.5621223182755166 1105 100-101 0.7355314242271227 1106 1 0.07086515807224458 1106 2 0.15296351574156475 1106 3 0.1302332151346235 1106 4 -0.048017471381463395 1106 5 0.03984327616238659 1106 6 0.012757297929518074 1106 7 -0.011354141022970055 1106 8 0.0809245167612076 1106 9 0.070536388394558 1106 10-11 0.01642747557514923 1106 12-13 -0.05866400435735386 1106 14-15 0.10629019205102708 1106 16-17 0.13268522161426688 1106 18-19 0.13732755290337195 1106 20-21 0.05686755843525759 1106 22-23 0.1298577362466844 1106 24-25 0.09543825938207107 1106 26-27 0.20768141714113852 1106 28-29 0.07784213230328163 1106 30-31 0.18443549657768443 1106 32-33 0.25895215380372605 1106 34-35 0.2678855533546809 1106 36-37 0.27124758769049606 1106 38-39 0.3537493129666984 1106 40-41 0.3904650099832665 1106 42-43 0.4454021101437249 1106 44-45 0.44472735094944227 1106 46-47 0.267417749060634 1106 48-49 0.4167722602485071 1106 50-51 0.32183354213145776 1106 52-53 0.33489524996612374 1106 54-55 0.37577933436673305 1106 56-57 0.16305074633938688 1106 58-59 0.3849399836854204 1106 60-61 0.22016381059903978 1106 62-63 0.20155743520773228 1106 64-65 0.34308625137622784 1106 66-67 0.15413395134263652 1106 68-69 0.2125165683015453 1106 70-71 0.3820630001902572 1106 72-73 0.32327011445988063 1106 74-75 0.3432513459800006 1106 76-77 0.26526858333874515 1106 78-79 0.38244329256365006 1106 80-81 0.35242753184379794 1106 82-83 0.3818530385289378 1106 84-85 0.5203278521181467 1106 86-87 0.7235014696074131 1106 88-89 0.5010218624470504 1106 90-91 0.5575603658341457 1106 92-93 0.6343896722558888 1106 94-95 0.6687837991982057 1106 96-97 0.5205375927606184 1106 98-99 0.4492891346876178 1106 100-101 0.5485742514208916 1107 1 -6.13042867346536E-4 1107 2 0.020277847527019333 1107 3 0.01636157952385986 1107 4 0.004459649882157635 1107 5 0.017922014786229568 1107 6 0.027674945715432386 1107 7 0.003944389849863228 1107 8 -0.04494341442794081 1107 9 0.08091434053110902 1107 10-11 0.14197293199341487 1107 12-13 0.03616347690751098 1107 14-15 0.12680122618957768 1107 16-17 0.10000582731903762 1107 18-19 0.05645872276458874 1107 20-21 0.07135153325342714 1107 22-23 0.051795197444370444 1107 24-25 0.046690842691759826 1107 26-27 0.1326288379516356 1107 28-29 0.13305578266618312 1107 30-31 0.060477716309065954 1107 32-33 0.12384634462264188 1107 34-35 0.18596373481702955 1107 36-37 0.212560925012923 1107 38-39 0.2178026113159106 1107 40-41 0.1769064148542654 1107 42-43 0.16451043170174984 1107 44-45 0.29279187025017706 1107 46-47 0.14713805399184565 1107 48-49 0.18737248704600518 1107 50-51 0.16171036262151262 1107 52-53 0.3118384794239546 1107 54-55 0.30034493568444987 1107 56-57 0.4312603289551973 1107 58-59 0.2229231574083812 1107 60-61 0.10682604338392565 1107 62-63 0.3754881533467511 1107 64-65 0.38979206917223763 1107 66-67 0.4257457305793295 1107 68-69 0.26543941875846144 1107 70-71 0.353889005679477 1107 72-73 0.5054113563026164 1107 74-75 0.4962707164717912 1107 76-77 0.5028960867027834 1107 78-79 0.4569934900048267 1107 80-81 0.6128936231826998 1107 82-83 0.515977728461543 1107 84-85 0.5065405632659612 1107 86-87 0.5106509143655522 1107 88-89 0.29562018178579663 1107 90-91 0.2693448229949844 1107 92-93 0.32615927333762684 1107 94-95 0.38335500022300906 1107 96-97 0.507181752912242 1107 98-99 0.6318853527315973 1107 100-101 0.6232145779268912 1108 1 0.014767155222894957 1108 2 0.0029932287414773384 1108 3 0.009211426306290349 1108 4 -0.035219380908728226 1108 5 -0.015691836285277816 1108 6 -0.013036969682048039 1108 7 0.007262833382959855 1108 8 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-0.9953707806570762 2316 50-51 -0.9479311599074904 2316 52-53 -0.7717443014894769 2316 54-55 -0.939610589718832 2316 56-57 -1.0595318550057513 2316 58-59 -0.9829172612621235 2316 60-61 -1.24598505354313 2316 62-63 -1.0281817033942815 2316 64-65 -0.9441512231522253 2316 66-67 -0.8505244337505289 2316 68-69 -0.6717149563982119 2316 70-71 -0.9221000422670933 2316 72-73 -1.1387144821400987 2316 74-75 -0.9798219018424064 2316 76-77 -0.720735843301135 2316 78-79 -0.7900915304728464 2316 80-81 -0.8012675282057735 2316 82-83 -1.1378146776270697 2316 84-85 -0.9873044264171433 2316 86-87 -1.1518543154473697 2316 88-89 -1.0635309203331573 2316 90-91 -1.0222355350319923 2316 92-93 -1.083753017458708 2316 94-95 -1.174759595348533 2316 96-97 -1.4520838029377003 2316 98-99 -1.3528851867174723 2316 100-101 -1.6875486000865756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 23.0 5 44.0 6 119.0 7 307.0 8 805.0 9 1597.0 10 2757.0 11 4436.0 12 6929.0 13 9725.0 14 11432.0 15 14619.0 16 18014.0 17 22736.0 18 21346.0 19 16931.0 20 16608.0 21 16755.0 22 17852.0 23 19484.0 24 22418.0 25 27017.0 26 34074.0 27 42641.0 28 53478.0 29 67135.0 30 83677.0 31 102404.0 32 127655.0 33 160226.0 34 210040.0 35 284192.0 36 415458.0 37 569956.0 38 500230.0 39 95317.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.922404206029235 19.194696351155528 14.293979608485866 19.588919834329374 2 14.211669045745007 25.159753998852707 36.308415267012634 24.320161688389653 3 26.95206684917064 24.621087383370117 29.007088294221766 19.41975747323748 4 11.845775964275411 16.016034710746556 42.34881165384635 29.789377671131685 5 11.45834827138281 40.66686787871916 34.42280210572304 13.451981744174995 6 26.919037717254824 39.312488431696565 16.84098189258278 16.92749195846583 7 26.38890510072553 33.41866503029019 22.24375895240779 17.948670916576482 8 23.662504022851465 28.885791038504316 19.6378183055166 27.81388663312762 9 21.551378274767842 22.945344247697594 25.31783444694755 30.18544303058701 10-11 25.491088029161297 24.26512300337336 28.08473917748737 22.15904978997797 12-13 31.034402258466333 22.751213525451934 25.683684648465317 20.53069956761642 14-15 23.048221072850794 26.237134813464486 30.522731657567803 20.191912456116917 16-17 19.83595103543611 28.89858078802866 29.881357147502328 21.384111029032898 18-19 18.81263767323274 33.659702617479766 28.055507846436296 19.4721518628512 20-21 22.4341494693851 27.02377865182874 25.9849791831524 24.557092695633756 22-23 19.93288632011831 27.984043325742302 27.615208231997755 24.46786212214163 24-25 24.481298117889143 27.58253723951516 27.123707059708284 20.812457582887415 26-27 23.404165552852014 23.98578942040893 32.50489981171182 20.105145215027235 28-29 19.39305520186104 32.28557481710588 28.91897557614961 19.40239440488347 30-31 21.537992791612496 27.000434264322216 27.278720314431382 24.182852629633903 32-33 22.094922583798553 27.703338534602786 28.948991705817935 21.25274717578073 34-35 25.378379568237065 25.42335487434263 28.95065623590874 20.24760932151157 36-37 23.61690268258586 24.6470935734488 31.957199244402148 19.778804499563194 38-39 21.089641427948678 28.257178639727453 31.416382374977413 19.236797557346456 40-41 21.10551406268839 29.539958639985603 28.41375623205967 20.940771065266343 42-43 19.527733550317045 30.31280576916951 25.84488811068571 24.31457256982773 44-45 22.31582398673028 25.560325052153804 26.60591937536513 25.51793158575079 46-47 24.973051593524644 28.898330466174084 26.914871051207978 19.2137468890933 48-49 23.89358631745946 27.723699819473335 28.082085104665776 20.300628758401427 50-51 18.381447196203474 28.587003412290883 32.402155731399695 20.629393660105947 52-53 21.62700500405791 31.38676053242549 25.976194831591414 21.01003963192519 54-55 19.703791625277415 27.51005640055315 28.033274375359245 24.75287759881019 56-57 23.538362324078076 25.494372482943344 28.112069714537487 22.855195478441097 58-59 27.723390460028657 25.48838521696671 25.936747060283206 20.85147726272143 60-61 22.580177693493233 27.497933001568555 31.604130861762414 18.3177584431758 62-63 20.774465036559185 27.783128977893725 30.06571414563439 21.3766918399127 64-65 19.553844426092372 32.84427974675032 27.318522666801055 20.283353160356256 66-67 22.16492515192555 27.202342933808314 25.64519672380267 24.987535190463458 68-69 20.1599730459345 28.865902690152506 26.79305832736346 24.18106593654953 70-71 24.377998765512192 28.001442292698357 26.125210952818783 21.495347988970664 72-73 23.207261648978612 24.662392852408335 31.08663770211198 21.043707796501074 74-75 19.83484014782112 32.4805927252026 27.545790563180322 20.138776563795965 76-77 21.694338802727454 27.000872277177034 26.55239407651927 24.75239484357624 78-79 22.119901810201405 27.93323347157567 28.24482483366147 21.70203988456145 80-81 25.03478442991554 26.531832255671894 27.562825416179194 20.870557898233365 82-83 22.91663887473353 25.499004159451882 30.743358617322425 20.840998348492164 84-85 20.73859680747128 28.62955584507363 29.881294440380334 20.75055290707476 86-87 21.507416732995445 29.585836608916022 26.997288316146218 21.90945834194232 88-89 20.128801410842517 29.76591233193219 25.340537843689425 24.76474841353587 90-91 22.317707925139178 25.095040105440408 26.625987044730465 25.961264924689953 92-93 24.193888075051298 28.672536858227453 27.00087460907309 20.132700457648163 94-95 22.879182736369714 27.463967401237195 28.15260225800728 21.504247604385814 96-97 17.862181490476818 28.20969281537104 32.12180923481671 21.80631645933544 98-99 21.201219200015476 30.751013401227507 26.065311618271725 21.982455780485296 100-101 19.337492931002078 27.35144764572142 27.865902255958847 25.445157167317657 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 200.0 1 165.0 2 310.5 3 491.0 4 381.5 5 300.0 6 434.0 7 768.0 8 1199.5 9 1447.0 10 1480.0 11 1475.5 12 1597.0 13 1806.0 14 2069.0 15 2345.0 16 2550.0 17 2777.0 18 3057.0 19 3341.0 20 3637.5 21 4061.5 22 4827.0 23 5954.0 24 7338.0 25 9208.0 26 11894.5 27 15421.5 28 20485.5 29 26693.5 30 33007.5 31 39344.5 32 45110.0 33 50349.5 34 55284.5 35 60330.5 36 65529.5 37 70900.0 38 75793.0 39 78411.0 40 80495.5 41 82998.5 42 85840.5 43 89616.0 44 90586.0 45 92616.5 46 104874.5 47 198686.0 48 403458.0 49 396900.5 50 199382.5 51 104860.0 52 70810.0 53 55719.5 54 47254.5 55 40710.0 56 34018.5 57 26683.0 58 20879.5 59 16770.0 60 13223.5 61 9844.0 62 7143.0 63 5044.0 64 3255.5 65 2059.0 66 1266.0 67 768.0 68 461.0 69 266.0 70 144.5 71 76.0 72 45.5 73 26.0 74 14.0 75 9.0 76 5.0 77 2.0 78 1.0 79 1.5 80 2.0 81 1.5 82 0.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04882449362096652 2 0.004502258633080928 3 0.043355083133371906 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001484077845719269 16-17 0.0 18-19 0.0 20-21 0.010755395623471107 22-23 1.667503197437381E-5 24-25 0.0 26-27 0.005519435583517732 28-29 0.01268969933249847 30-31 0.010705370527547987 32-33 0.006286487054338926 34-35 0.006103061702620815 36-37 0.020143438625043565 38-39 0.04757386622288848 40-41 0.006286487054338926 42-43 0.014023701890448374 44-45 0.013056550035934695 46-47 0.005769561063133338 48-49 0.0015341029416423904 50-51 0.00231782944443796 52-53 9.504768225393072E-4 54-55 0.0010505270143855501 56-57 0.010855445815317352 58-59 0.008237465795340662 60-61 0.006203111894467058 62-63 0.0068701131734420105 64-65 0.07215286335311548 66-67 0.035234342561851865 68-69 0.026763426318869965 70-71 0.01635820636686071 72-73 8.337515987186906E-5 74-75 2.0010038369248575E-4 76-77 7.170263748980739E-4 78-79 5.502760551543358E-4 80-81 9.004517266161859E-4 82-83 7.670514708211952E-4 84-85 8.504266306930643E-4 86-87 0.005786236095107712 88-89 1.0005019184624288E-4 90-91 8.00401534769943E-4 92-93 4.1687579935934525E-4 94-95 0.003301656330926014 96-97 0.007770564900058195 98-99 0.009988344152649913 100-101 0.012289498565113497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2998495.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.92445036773475 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87446167367226 35.08441827434749 2 12.740082594109323 11.192022511715928 3 3.1733386401778043 4.181614668015145 4 1.259069755506962 2.2121578794112593 5 0.6738395717046302 1.4799016411577834 6 0.43584942864132137 1.1486667957696761 7 0.295785031679281 0.90945364604608 8 0.2175356707856095 0.7644107819707513 9 0.15437661690292354 0.6102817242382139 >10 1.0156456354065528 8.573294749386653 >50 0.09766557672395045 2.930586506864564 >100 0.05279346605612427 4.269741437590177 >500 0.004281239707727859 1.2718634736496126 >1k 0.0029815776535959543 2.8644548162581938 >5k 9.174085087987552E-4 2.843836356249723 >10k+ 0.0013761127631981328 19.663294737328783 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 136992 4.568691960466834 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 59932 1.9987360325763426 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 50828 1.6951170503869442 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 44458 1.4826771430334218 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 36254 1.2090732183978963 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 32465 1.0827098260960915 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 30021 1.0012022698053524 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 28354 0.9456077132027901 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 26709 0.8907468580071002 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 22834 0.7615153602057032 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 22257 0.7422723733072758 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 20781 0.6930476789189243 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 14699 0.49021258998264133 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 12646 0.4217449086958624 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 12257 0.4087717338197996 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 12102 0.40360247390774373 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 11325 0.3776894742195668 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 10642 0.35491138054257215 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 9397 0.3133905509263814 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 9385 0.31299035015899646 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 8473 0.2825750918377386 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7976 0.26600011005521107 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7799 0.2600971487362827 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 6952 0.23184964457169344 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6437 0.21467436163808842 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6311 0.21047225358054622 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 6290 0.20977190223762254 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 5461 0.18212469922411076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5108 0.17035212665020286 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5098 0.17001862601071538 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 4687 0.1563117497277801 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3608 0.12032703072708142 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3586 0.11959332932020897 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3551 0.1184260770820028 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3455 0.11522447094292304 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3338 0.11132251346091955 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3191 0.10642005406045366 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3168 0.10565300258963245 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3005 0.10021694216598662 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.335006394874762E-5 0.0 5 0.0 0.0 0.0 3.335006394874762E-5 0.0 6 0.0 0.0 0.0 3.335006394874762E-5 0.0 7 0.0 0.0 0.0 6.670012789749524E-5 0.0 8 0.0 0.0 0.0 6.670012789749524E-5 0.0 9 0.0 0.0 0.0 6.670012789749524E-5 0.0 10-11 0.0 3.335006394874762E-5 0.0 1.1672522382061668E-4 0.0 12-13 0.0 3.335006394874762E-5 0.0 2.3345044764123336E-4 0.0 14-15 0.0 1.0005019184624288E-4 0.0 2.834755435643548E-4 0.0 16-17 0.0 1.6675031974373812E-4 0.0 3.8352573541059766E-4 0.0 18-19 0.0 1.6675031974373812E-4 0.0 5.669510871287096E-4 0.0 20-21 0.0 1.6675031974373812E-4 0.0 0.0010838770783342976 0.0 22-23 0.0 1.6675031974373812E-4 0.0 0.0018342535171811192 0.0 24-25 0.0 1.6675031974373812E-4 0.0 0.0028514304676179217 0.0 26-27 0.0 1.6675031974373812E-4 0.0 0.0037685572262084813 0.0 28-29 0.0 2.0010038369248573E-4 0.0 0.006436562342108291 0.0 30-31 0.0 2.0010038369248573E-4 0.0 0.011655847350087293 0.0 32-33 0.0 2.0010038369248573E-4 0.0 0.02227784271776341 0.0 34-35 0.0 2.0010038369248573E-4 0.0 0.034117115419568814 0.0 36-37 0.0 2.0010038369248573E-4 0.0 0.04622318863296421 0.0 38-39 3.335006394874762E-5 2.0010038369248573E-4 0.0 0.06276482035154302 0.0 40-41 3.335006394874762E-5 2.0010038369248573E-4 0.0 0.0799067532211993 0.0 42-43 3.335006394874762E-5 3.001505755387286E-4 0.0 0.09611488430029064 0.0 44-45 3.335006394874762E-5 3.001505755387286E-4 0.0 0.10722045559522361 0.0 46-47 3.335006394874762E-5 3.001505755387286E-4 0.0 0.12191115876464693 0.0 48-49 3.335006394874762E-5 3.001505755387286E-4 0.0 0.1324331039404768 0.0 50-51 3.335006394874762E-5 3.668507034362238E-4 0.0 0.1430550993081529 0.0 52-53 3.335006394874762E-5 3.668507034362238E-4 0.0 0.16628341884845566 0.0 54-55 3.335006394874762E-5 3.668507034362238E-4 0.0 0.2022848128811287 0.0 56-57 6.670012789749524E-5 3.668507034362238E-4 0.0 0.2637656557706449 0.0 58-59 6.670012789749524E-5 3.668507034362238E-4 0.0 0.3509260479006968 0.0 60-61 6.670012789749524E-5 3.668507034362238E-4 0.0 0.44877513552632237 0.0 62-63 6.670012789749524E-5 3.668507034362238E-4 0.0 0.4916633177644118 0.0 64-65 6.670012789749524E-5 3.668507034362238E-4 0.0 0.5488753524684884 0.0 66-67 6.670012789749524E-5 3.668507034362238E-4 0.0 0.7435396757373283 0.0 68-69 6.670012789749524E-5 3.668507034362238E-4 0.0 0.953144827655207 0.0 70-71 6.670012789749524E-5 3.8352573541059766E-4 0.0 1.0841939039418107 0.0 72-73 6.670012789749524E-5 4.0020076738497146E-4 0.0 1.223597171247576 0.0 74-75 6.670012789749524E-5 4.0020076738497146E-4 0.0 1.350694264956253 0.0 76-77 6.670012789749524E-5 4.0020076738497146E-4 0.0 1.437637881670638 0.0 78-79 6.670012789749524E-5 4.335508313337191E-4 0.0 1.5223637191324313 0.0 80-81 6.670012789749524E-5 4.335508313337191E-4 0.0 1.6069728313704041 0.0 82-83 6.670012789749524E-5 4.335508313337191E-4 0.0 1.7322856966578235 0.0 84-85 8.337515987186906E-5 4.669008952824667E-4 0.0 2.0532467121005706 0.0 86-87 1.0005019184624286E-4 4.669008952824667E-4 0.0 2.6036228174467526 0.0 88-89 1.0005019184624286E-4 4.835759272568405E-4 0.0 3.263920733568007 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3405 0.0 49.663628 2 ACCTGGG 3535 0.0 47.163803 3 GTACCTG 4000 0.0 43.25505 1 AGTACGC 2230 0.0 35.359722 2 TACGCAG 2315 0.0 34.254032 4 GTACGCA 2360 0.0 33.41082 3 GAGTACG 2425 0.0 33.32212 1 CCTGGGG 2295 0.0 32.06973 4 ACGCAGT 2660 0.0 29.811312 5 CGCAGTG 2675 0.0 29.466633 6 TACACGG 325 0.0 27.769995 2 GTACCGG 190 3.929017E-10 27.519121 1 TATAACG 435 0.0 27.299604 2 TAACGCA 505 0.0 26.327698 4 GTACCCG 390 0.0 25.594706 1 GTACACG 355 0.0 25.44021 1 TCACGCA 390 0.0 23.133173 4 TATCACG 350 0.0 23.072063 2 GTAGGCA 1795 0.0 22.757528 3 ACACGGG 365 0.0 22.12316 3 >>END_MODULE