FastQCFastQC Report
Wed 25 May 2016
SRR1003836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2954156
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT283670.9602404206142126No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT198510.671968575796268No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC124230.420526201053702No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT113870.3854569630039849No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA94540.3200237225116074No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA88100.2982239258861076No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT76020.2573323819053564No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73600.24914053286285492No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT71210.24105023566798775No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC60820.20587944577063633No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT57930.19609661778186394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56390.19088362293663572No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC54510.18451970715155191No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC52960.1792728616904456No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG48700.16485249932637275No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA42160.1427141965420919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40630.13753505231274177No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA40140.13587637213471462No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34580.11705542970648808No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33040.11184243486125986No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA30800.10425989690456428No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC30770.10415834505692997No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG29620.10026552423094785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG41900.054.5175632
ACCTGGG45700.049.7995873
GTACCCG4750.049.6981851
GTACCTG53150.044.198461
GTACCGG2500.041.455561
TATAACG4650.038.3591232
TAACGCA5800.034.677054
TACCCGG6650.032.879252
CCTGGGG29800.031.0464364
ATAACGC6900.029.984733
GTACACG5500.029.312011
TACCGGG3700.027.991792
GAGTACG8150.026.1393241
CTGGGGG19800.023.8001965
AGTACGC7000.023.0154722
ACCCGGG9800.021.6981333
TACACGG6700.021.469662
GAGTACT78300.021.32124312-13
GTACCGT1350.003126747221.2850936
GTATAAC12300.021.0648161
Produced by FastQC (version 0.11.5)