FastQCFastQC Report
Wed 25 May 2016
SRR1003836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2954156
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT343701.1634456677304787No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT158030.5349412827216978No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC114150.38640478024857183No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT109620.37107045125579013No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA107390.36352176391497265No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA96920.32808016909059645No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT91960.31129026361505624No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC83790.2836343104426442No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80230.2715834911900387No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT76620.2593634188580427No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC73970.2503930056503448No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG65780.2226693512461766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63100.21359738619084437No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT60030.20320524711626603No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA58290.1973152399534757No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC55180.18678769841538495No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA54900.18583988117079803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39300.13303292040095377No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC38100.12897084649558113No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG34630.11722468278587861No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC31130.10537696722854176No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA30730.10402294259341754No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT30130.10199190564073121No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT30110.10192420440897501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG39000.050.4371382
ACCTGGG41050.046.411313
GTACCTG46300.043.6402741
TATAACG4500.041.1781432
GTACCCG4500.038.0337831
TAACGCA5150.035.0381434
ATAACGC6100.033.491243
TACCCGG5350.031.9715292
GTACCGG2700.031.694821
CCTGGGG28100.031.0939714
GTACACG4650.028.6275791
TACACGG4400.028.0760082
CTGGGGG18400.025.0333275
GCGCTAA1151.2747057E-424.804641
TACCGGG2853.6379788E-1223.3398382
ACACGGG5000.022.8051993
GTATAAC9200.022.2208231
TCACGCA4600.021.6783454
ACCCGGG7650.019.8738123
CCTGGGA25200.019.7857934
Produced by FastQC (version 0.11.5)