##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003835_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1295918 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.661879069509027 31.0 31.0 34.0 30.0 34.0 2 31.84811384670944 31.0 31.0 34.0 30.0 34.0 3 31.90597476074875 31.0 31.0 34.0 30.0 34.0 4 35.68467294998603 37.0 35.0 37.0 33.0 37.0 5 35.52888377196705 37.0 35.0 37.0 33.0 37.0 6 35.57259332766425 37.0 35.0 37.0 33.0 37.0 7 35.516717878754676 37.0 35.0 37.0 33.0 37.0 8 35.51160798754242 37.0 35.0 37.0 33.0 37.0 9 37.09660101950895 39.0 37.0 39.0 33.0 39.0 10-11 37.01493420108371 39.0 37.0 39.0 33.0 39.0 12-13 36.96871869979428 39.0 37.0 39.0 33.0 39.0 14-15 38.10742114856033 40.0 37.0 41.0 33.0 41.0 16-17 38.08019836131607 40.0 37.0 41.0 33.0 41.0 18-19 38.12917561141985 40.0 37.0 41.0 33.0 41.0 20-21 38.08432362232796 40.0 37.0 41.0 33.0 41.0 22-23 38.01122640475709 40.0 37.0 41.0 33.0 41.0 24-25 37.90816548577919 40.0 37.0 41.0 33.0 41.0 26-27 37.714280147355 40.0 37.0 41.0 32.0 41.0 28-29 37.51934883225636 39.0 36.5 41.0 32.0 41.0 30-31 37.33401688995754 39.0 36.0 41.0 31.5 41.0 32-33 37.10724984142515 39.0 36.0 40.5 31.0 41.0 34-35 37.096398460396415 39.0 36.0 41.0 31.0 41.0 36-37 36.928447633260745 39.0 35.5 41.0 31.0 41.0 38-39 36.66015095090893 39.0 35.0 40.0 30.0 41.0 40-41 36.64758919931663 39.0 35.0 41.0 30.0 41.0 42-43 36.55578207880437 39.0 35.0 41.0 30.0 41.0 44-45 36.30316385758975 39.0 35.0 41.0 30.0 41.0 46-47 36.13852535422766 39.0 35.0 41.0 29.5 41.0 48-49 35.92157644233663 39.0 35.0 41.0 28.5 41.0 50-51 35.7046213572155 39.0 35.0 40.5 28.0 41.0 52-53 35.43194206732216 39.0 35.0 40.0 27.0 41.0 54-55 35.2371106813857 38.0 34.0 40.0 27.0 41.0 56-57 35.04711949367167 38.0 34.0 40.0 26.0 41.0 58-59 34.80738634697566 38.0 34.0 40.0 26.0 41.0 60-61 34.505303576306524 38.0 34.0 40.0 25.5 41.0 62-63 34.18772175399987 37.5 33.0 40.0 24.5 41.0 64-65 33.67737966445408 37.0 32.5 40.0 23.0 41.0 66-67 33.35263496610125 36.0 32.0 39.0 22.0 41.0 68-69 33.134557510583235 36.0 32.0 39.0 22.0 41.0 70-71 32.74800025927566 35.5 32.0 39.0 22.0 40.0 72-73 32.364168874882516 35.0 31.5 38.0 21.0 40.0 74-75 31.96660514014004 35.0 31.0 37.0 20.0 39.5 76-77 30.86835895481041 34.0 30.0 36.0 19.0 39.0 78-79 31.27267851823958 35.0 31.0 36.0 19.5 39.0 80-81 31.13303465188384 35.0 31.0 36.0 18.5 38.5 82-83 30.85379514753248 34.5 31.0 36.0 18.0 37.0 84-85 30.49882824376234 34.0 31.0 35.0 16.0 37.0 86-87 30.114900016822055 34.0 30.0 35.0 10.5 36.5 88-89 29.747240566146935 34.0 30.0 35.0 7.0 36.0 90-91 29.46942514881343 34.0 30.0 35.0 4.0 36.0 92-93 29.25769223052693 34.0 30.0 35.0 2.0 36.0 94-95 28.94567866176718 34.0 29.0 35.0 2.0 35.0 96-97 28.57815965207675 34.0 29.0 35.0 2.0 35.0 98-99 28.171868513285563 34.0 29.0 35.0 2.0 35.0 100-101 27.310675521136368 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40874450532536954 1101 2 0.378470475322942 1101 3 0.3247569116150544 1101 4 0.10958707566830128 1101 5 0.337681510139646 1101 6 0.32990388561968587 1101 7 0.27466951438385223 1101 8 0.2643250456818578 1101 9 0.37807768053229296 1101 10-11 0.48635385751410354 1101 12-13 0.4483322741903777 1101 14-15 0.6373096334739117 1101 16-17 0.5488848993702291 1101 18-19 0.5451349383080952 1101 20-21 0.3319326418055937 1101 22-23 0.44530699041188626 1101 24-25 0.515989236359907 1101 26-27 0.4973158375410591 1101 28-29 0.6361642930829063 1101 30-31 0.592066548351518 1101 32-33 0.6130666657508712 1101 34-35 0.6895594820781312 1101 36-37 0.7328349379674748 1101 38-39 0.6409784166475987 1101 40-41 0.8084462778263131 1101 42-43 0.7862362739509692 1101 44-45 0.9075428416477962 1101 46-47 0.924527259465485 1101 48-49 0.7713624885272736 1101 50-51 0.8399684590617369 1101 52-53 0.9025935123882007 1101 54-55 0.8726433273028746 1101 56-57 0.8478413273606833 1101 58-59 0.771516030351826 1101 60-61 0.8398908299969534 1101 62-63 0.8681358695333472 1101 64-65 0.6802175432364734 1101 66-67 0.719856758307678 1101 68-69 0.8502518425549752 1101 70-71 0.8008103071164925 1101 72-73 0.7610764152043146 1101 74-75 0.7113395466678405 1101 76-77 0.8487861711607501 1101 78-79 0.8149244390581316 1101 80-81 0.8455897697843326 1101 82-83 1.024237678591728 1101 84-85 1.0550051459608198 1101 86-87 0.8625802836672349 1101 88-89 0.978506059661175 1101 90-91 1.0999134974983846 1101 92-93 1.103237557038387 1101 94-95 1.0512314050313059 1101 96-97 0.9782896600445632 1101 98-99 1.0929245755294943 1101 100-101 1.1000459838489505 1102 1 0.24044542892832155 1102 2 0.32772227895446093 1102 3 0.2961434914393948 1102 4 0.17410733563730219 1102 5 0.26186987309463916 1102 6 0.21613852225102193 1102 7 0.24577733712178684 1102 8 0.2752130367854093 1102 9 0.44803130257930945 1102 10-11 0.3853010978761233 1102 12-13 0.3190101387052877 1102 14-15 0.3392992135843329 1102 16-17 0.36224811162146153 1102 18-19 0.34850931685015496 1102 20-21 0.27402926097836655 1102 22-23 0.3724500870120764 1102 24-25 0.3465195631414133 1102 26-27 0.3344031301282868 1102 28-29 0.31453041067357645 1102 30-31 0.2622778206112102 1102 32-33 0.42413499142238464 1102 34-35 0.5729339429438625 1102 36-37 0.3840009726856479 1102 38-39 0.3904960799909034 1102 40-41 0.5823938326189975 1102 42-43 0.6583907049194906 1102 44-45 0.6804389402605793 1102 46-47 0.4147621860590718 1102 48-49 0.5328585885578505 1102 50-51 0.6379065027266719 1102 52-53 0.637745581353478 1102 54-55 0.6912917190299837 1102 56-57 0.5949161501990048 1102 58-59 0.6374847523889358 1102 60-61 0.620137403102774 1102 62-63 0.33145175710939867 1102 64-65 0.42134505276487033 1102 66-67 0.48049600616205623 1102 68-69 0.5069078368728199 1102 70-71 0.3627570377577882 1102 72-73 0.5176063399719197 1102 74-75 0.36808483929760527 1102 76-77 0.45492984928618796 1102 78-79 0.510190919627572 1102 80-81 0.48075860076309596 1102 82-83 0.38334483725207846 1102 84-85 0.45327207267880354 1102 86-87 0.5286152305215914 1102 88-89 0.41993299866368616 1102 90-91 0.7219822318154385 1102 92-93 0.8036443502959862 1102 94-95 0.7425640720002846 1102 96-97 0.7285916850009819 1102 98-99 0.7869381356096454 1102 100-101 0.9990774916320859 1103 1 -0.08044984083077011 1103 2 0.10694000400930648 1103 3 -0.0051677202825217705 1103 4 0.08358248678004543 1103 5 0.08605932892946555 1103 6 0.05981586486740298 1103 7 0.0845663589293224 1103 8 0.11479835658946058 1103 9 0.1754224662104633 1103 10-11 0.16620848777798614 1103 12-13 0.16094793565006427 1103 14-15 0.14798124679341385 1103 16-17 0.23822719911780865 1103 18-19 0.07452689849442606 1103 20-21 0.08382508555584423 1103 22-23 0.08021308205682232 1103 24-25 0.16509441284497228 1103 26-27 0.06476136933774512 1103 28-29 0.1422620461952846 1103 30-31 0.1500507097547299 1103 32-33 0.32242645136359016 1103 34-35 0.40459308431663743 1103 36-37 0.28401902723603456 1103 38-39 0.3681362630599381 1103 40-41 0.4052585614647839 1103 42-43 0.6088952065036253 1103 44-45 0.6051572354186234 1103 46-47 0.5052429243670815 1103 48-49 0.4264358617770512 1103 50-51 0.6455523740049003 1103 52-53 0.632741213896189 1103 54-55 0.7311613723569721 1103 56-57 0.5617454018532797 1103 58-59 0.5512935085381301 1103 60-61 0.4315480071762252 1103 62-63 0.4515655520681747 1103 64-65 0.3400854709020962 1103 66-67 0.3109913471135215 1103 68-69 0.15724633404692412 1103 70-71 0.2510916952624598 1103 72-73 0.3116738044924148 1103 74-75 0.12539205952773003 1103 76-77 0.2139201947179501 1103 78-79 0.4152698903335512 1103 80-81 0.39507629519701126 1103 82-83 0.5361039008158919 1103 84-85 0.4348197579777313 1103 86-87 0.5515656671903812 1103 88-89 0.35158883174526423 1103 90-91 0.49749005451359807 1103 92-93 0.41848911450201953 1103 94-95 0.34521560327748446 1103 96-97 0.5035535114239167 1103 98-99 0.49102238778452545 1103 100-101 0.40411376230056817 1104 1 -0.005506514812029906 1104 2 0.04756357506287401 1104 3 0.11333957051972021 1104 4 0.07793746792097522 1104 5 -0.06888382773266244 1104 6 0.10773289948147635 1104 7 -0.08674874356771767 1104 8 -0.21618555695157227 1104 9 -0.01635333466486344 1104 10-11 0.012087315539886845 1104 12-13 -0.026341729483519316 1104 14-15 -0.06236722863415167 1104 16-17 0.08173019552018701 1104 18-19 -0.0176006802624471 1104 20-21 0.031238042304579494 1104 22-23 -0.20474030858356684 1104 24-25 -0.016734711170037997 1104 26-27 0.06729718221320269 1104 28-29 0.17639405725668666 1104 30-31 -0.05313246877859257 1104 32-33 0.07497587325268995 1104 34-35 0.17019143333158837 1104 36-37 0.1508929339786036 1104 38-39 0.2890796882732687 1104 40-41 0.3844146937468125 1104 42-43 0.23638626084532177 1104 44-45 0.4328468603904909 1104 46-47 0.42908174283856937 1104 48-49 0.3628938649657911 1104 50-51 0.4726483435594204 1104 52-53 0.49202582736391065 1104 54-55 0.4662903495155959 1104 56-57 0.5257063143467136 1104 58-59 0.33028826816060786 1104 60-61 0.4710994298927531 1104 62-63 0.3480203132874493 1104 64-65 0.3793952580208355 1104 66-67 0.16421450590470243 1104 68-69 0.29758883360538135 1104 70-71 0.12663121704776614 1104 72-73 0.4739697600145334 1104 74-75 0.49588021787132064 1104 76-77 0.3739389086238205 1104 78-79 0.4193577356093492 1104 80-81 0.4204002502062174 1104 82-83 0.5814911710050161 1104 84-85 0.528133437879216 1104 86-87 0.5826117588026349 1104 88-89 0.5632776438053888 1104 90-91 0.2570877357796135 1104 92-93 0.49052585762916223 1104 94-95 0.6106648579874125 1104 96-97 0.5724756049619977 1104 98-99 0.6521503875135579 1104 100-101 0.7940845845753195 1105 1 -0.08217752520518928 1105 2 -0.11851245074521799 1105 3 -0.0701712199325577 1105 4 -0.07228978313582246 1105 5 -0.006243583636418748 1105 6 0.052750857082465075 1105 7 -0.19284161455904325 1105 8 0.010299520758756842 1105 9 -0.16101531858248563 1105 10-11 -0.2148141242465229 1105 12-13 -0.1115680270393753 1105 14-15 -0.08183612861190426 1105 16-17 -0.05691042997642626 1105 18-19 -0.14272066246375914 1105 20-21 -0.21985133251664024 1105 22-23 -0.19746986393429466 1105 24-25 -0.09640050909140285 1105 26-27 -0.30888198061297345 1105 28-29 -0.08508450486389307 1105 30-31 -0.14092447844073064 1105 32-33 -0.035573820037832604 1105 34-35 0.04423554864163037 1105 36-37 0.07228234376152187 1105 38-39 0.16709672415668564 1105 40-41 0.31682419061615263 1105 42-43 0.36241842561027937 1105 44-45 0.28417540503023275 1105 46-47 0.19255312191875618 1105 48-49 0.22259017491716548 1105 50-51 0.22913294451927868 1105 52-53 0.25925320555416675 1105 54-55 0.27660325673996056 1105 56-57 0.33099347704575877 1105 58-59 0.29644613613250925 1105 60-61 0.36242813344931335 1105 62-63 0.20296898315363876 1105 64-65 0.16281069837935291 1105 66-67 0.3495282077131776 1105 68-69 0.2438948120562614 1105 70-71 0.3120823819080769 1105 72-73 0.3737186714342897 1105 74-75 0.34272889727252576 1105 76-77 0.33449813692372743 1105 78-79 0.5431745998625992 1105 80-81 0.5462258231013628 1105 82-83 0.5042313737828827 1105 84-85 0.33592036220302646 1105 86-87 0.5805649101232326 1105 88-89 0.6174333187251371 1105 90-91 0.4451713578118408 1105 92-93 0.36927867840277173 1105 94-95 0.3882130892431732 1105 96-97 0.5055565254082346 1105 98-99 0.4879026997533309 1105 100-101 0.5181366457265142 1106 1 -0.093062841632225 1106 2 -0.0990683207780485 1106 3 -0.04534386795701195 1106 4 -0.09773413952662935 1106 5 -0.10228683055139953 1106 6 -0.09632646659012778 1106 7 -0.020408692561986186 1106 8 0.02384531493967046 1106 9 0.01907072240019403 1106 10-11 -0.1294976972115407 1106 12-13 -0.19933265966702862 1106 14-15 -0.17935395947343835 1106 16-17 -0.24858370066588265 1106 18-19 -0.2941754343419518 1106 20-21 -0.09961755010975537 1106 22-23 -0.19143567396486816 1106 24-25 -0.17633088382252282 1106 26-27 0.008184701148600482 1106 28-29 -0.11432711779268345 1106 30-31 -0.20012293875999632 1106 32-33 -0.055145313301729004 1106 34-35 0.012564542188563621 1106 36-37 -0.05551681847101264 1106 38-39 -0.04967843717015086 1106 40-41 -0.08180311607465995 1106 42-43 -0.10599547680113375 1106 44-45 -0.18393565576451465 1106 46-47 -0.29473036047680523 1106 48-49 -0.2206539097652751 1106 50-51 -0.14072723888386207 1106 52-53 -0.09963109196649356 1106 54-55 -0.2599924638532727 1106 56-57 -0.5124175950701684 1106 58-59 -0.12583961049944037 1106 60-61 -0.24213713316451901 1106 62-63 -0.440504355462906 1106 64-65 -0.23143531090401837 1106 66-67 -0.5895864758597895 1106 68-69 -0.7198264604445868 1106 70-71 -0.5018624839359163 1106 72-73 -0.4077683658123945 1106 74-75 -0.33317962757746855 1106 76-77 -0.35405048755266577 1106 78-79 -0.31786638281599267 1106 80-81 -0.3443238202814065 1106 82-83 -0.36820154821417717 1106 84-85 -0.31851188052311485 1106 86-87 -0.21033852019489174 1106 88-89 -0.3470323214877027 1106 90-91 -0.18227967807630563 1106 92-93 -0.1730314087703313 1106 94-95 -0.2146879636267478 1106 96-97 -0.3521730115554256 1106 98-99 -0.5788696312780282 1106 100-101 -0.6217818417635783 1107 1 -0.17138023414777592 1107 2 -0.30445724577882416 1107 3 -0.18307717652790956 1107 4 -0.01865220681854396 1107 5 -0.18628565433571964 1107 6 -0.08522051431972244 1107 7 -0.1500911143281698 1107 8 -0.2079063178334124 1107 9 -0.18202501536561044 1107 10-11 -0.16472690450407868 1107 12-13 -0.2872029354586445 1107 14-15 -0.14353896581881287 1107 16-17 -0.3135301518993643 1107 18-19 -0.23308031563702514 1107 20-21 -0.1712622401150128 1107 22-23 -0.1754468789668735 1107 24-25 -0.18964610962498085 1107 26-27 -0.1971214244588495 1107 28-29 -0.15844913469304345 1107 30-31 -0.0063290241284832405 1107 32-33 -0.1089564068727853 1107 34-35 -0.2634692894887891 1107 36-37 -0.06615847507793404 1107 38-39 -0.023464304010154535 1107 40-41 0.0745623774716293 1107 42-43 -0.06156315563234216 1107 44-45 -0.07730938282056599 1107 46-47 -0.05514838133915845 1107 48-49 -0.04579010315989507 1107 50-51 0.08764199998077515 1107 52-53 -0.15076552103759155 1107 54-55 -0.2521536054015385 1107 56-57 -0.20309918110415737 1107 58-59 -0.23546987638609096 1107 60-61 -0.3623935791359827 1107 62-63 -0.0016575111257708386 1107 64-65 -0.025312024928844323 1107 66-67 -0.15733649907456737 1107 68-69 -0.20090022154898435 1107 70-71 -0.2370274636616685 1107 72-73 0.03332566953598359 1107 74-75 -0.1691060521913279 1107 76-77 -0.23044337454309982 1107 78-79 -0.13617356082695053 1107 80-81 -0.12542776060393734 1107 82-83 -0.20218953843301435 1107 84-85 -0.2856575455492667 1107 86-87 -0.1802003148523994 1107 88-89 -0.3429612223450995 1107 90-91 -0.2423626322348511 1107 92-93 -0.06927757833110704 1107 94-95 -0.29802671374281786 1107 96-97 -0.30946322729814213 1107 98-99 -0.16454843153803722 1107 100-101 -0.01802491601993239 1108 1 -0.1422724102121009 1108 2 -0.12583304896062586 1108 3 -0.21853743835658435 1108 4 -0.11996478627770557 1108 5 -0.19366032361379837 1108 6 -0.24430205230549262 1108 7 -0.12175640656607811 1108 8 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-0.8215076424362202 2316 74-75 -0.5757863899820173 2316 76-77 -0.3360367867702152 2316 78-79 -0.3571170819958347 2316 80-81 -0.2707724580591133 2316 82-83 -0.4504290251962324 2316 84-85 -0.2850078111393337 2316 86-87 -0.571222943410806 2316 88-89 -0.4797240853063265 2316 90-91 -0.5202317881853489 2316 92-93 -0.5846629331394944 2316 94-95 -0.5617191988968315 2316 96-97 -0.6392986753834755 2316 98-99 -0.3885465944807436 2316 100-101 -0.5427415250115466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 10.0 5 21.0 6 65.0 7 164.0 8 393.0 9 863.0 10 1419.0 11 2255.0 12 3364.0 13 4736.0 14 5689.0 15 7668.0 16 9456.0 17 12163.0 18 11220.0 19 7671.0 20 6923.0 21 7017.0 22 7349.0 23 8236.0 24 9625.0 25 11493.0 26 14455.0 27 17898.0 28 22626.0 29 28454.0 30 34993.0 31 43163.0 32 53536.0 33 66835.0 34 86640.0 35 114354.0 36 161811.0 37 234106.0 38 241958.0 39 57252.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03284226554724 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19.414808653016628 29.794709233145923 31.167249779692852 19.6232323341446 20-21 20.786683125355243 28.158981979433843 29.87873228996361 21.175602605247306 22-23 20.329411274478787 27.783818111948438 29.990747871393097 21.896022742179674 24-25 21.033892576536477 27.99444100629824 30.464196037094936 20.507470380070345 26-27 21.40119003108774 27.010140460092362 31.13392499200331 20.454744516816586 28-29 20.333964749022876 27.74371597329918 31.799233734807725 20.123085542870218 30-31 20.492429197241396 28.808717082606467 30.52159649575687 20.177257224395266 32-33 20.182751364153045 28.611484737151187 30.306229304268445 20.899534594427326 34-35 21.188602075857546 27.12185052282286 30.68962457074507 20.999922830574526 36-37 21.827670134267425 27.49952630337963 30.866727819517536 19.80607574283541 38-39 21.76816709434568 26.98426851448705 31.50956024715833 19.73800414400894 40-41 20.45001090011826 27.602880717980177 31.711009891133862 20.236098490767695 42-43 20.054599159211534 28.916795125819228 30.274828630024135 20.753777084945103 44-45 20.54848751908061 28.595296561708512 29.520580822306837 21.335635096904042 46-47 20.882802899204975 28.22137208714592 29.010724434695547 21.885100578953562 48-49 21.52502338128941 28.050602357574768 29.44275365226544 20.981620608870383 50-51 21.72934039506769 28.466333746043787 29.479371357997447 20.324954500891078 52-53 20.806482852603303 28.93146211429643 29.510745722507174 20.75130931059309 54-55 20.14189300767185 28.83237673102833 29.83588315819203 21.189847103107788 56-57 21.29670431437314 28.91559942428721 28.584834675505583 21.20286158583407 58-59 21.33400111975126 28.058759545570826 29.19699326335724 21.41024607132067 60-61 20.978785697033114 27.88668779531296 29.96602285258332 21.168503655070605 62-63 21.937491005018668 27.904795381296537 29.24108787860431 20.916625735080487 64-65 21.255746510581687 28.35402173682188 30.00361057531718 20.38662117727925 66-67 20.40085177238363 28.092972414663546 29.743870021726604 21.76230579122622 68-69 20.30310784144594 29.129196929015617 29.479898621854655 21.08779660768379 70-71 20.743962008874167 28.80580795587769 28.74962085282452 21.700609182423626 72-73 20.515179996234323 28.04344571716068 29.09833662058343 22.343037666021562 74-75 20.911402368210876 28.759640871507774 29.215496386722894 21.11346037355845 76-77 21.339533333230445 28.047154363638327 28.986838632810514 21.626473670320713 78-79 20.644896437918117 28.036091915085382 29.880350810451468 21.438660836545033 80-81 20.59517112645679 29.110326078988326 28.809109870801514 21.48539292375337 82-83 20.45942091836341 28.75765675808734 29.20849686013115 21.5744254634181 84-85 20.736893887511695 27.998572434481495 28.674814078882644 22.58971959912417 86-87 21.230250902474147 28.108886457254595 28.474814836536044 22.186047803735217 88-89 21.517019621642145 27.175102398612257 29.415023813271844 21.892854166473754 90-91 20.61214198378975 26.816978204865542 30.107449715778543 22.46343009556616 92-93 19.998387239281605 28.129286307082758 29.533854278195047 22.338472175440586 94-95 19.912107246136365 28.421615135456314 29.156549804286218 22.509727814121103 96-97 19.629221780905322 28.43319810875945 28.855077172152434 23.082502938182795 98-99 20.186171957294274 28.288182614433055 28.43279867576755 23.09284675250512 100-101 20.141601948734213 28.19355841938918 28.544611482113 23.120228149763605 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 138.0 1 97.0 2 127.0 3 184.0 4 160.5 5 150.0 6 219.5 7 391.5 8 632.5 9 799.5 10 803.5 11 830.0 12 949.5 13 1095.5 14 1227.0 15 1336.5 16 1462.5 17 1611.5 18 1826.5 19 2021.0 20 2206.0 21 2505.5 22 2935.5 23 3504.5 24 4265.0 25 5331.0 26 6856.0 27 9048.0 28 12042.0 29 15792.5 30 19847.0 31 24027.0 32 28088.0 33 31708.0 34 34960.0 35 37950.0 36 40858.5 37 43834.5 38 46314.0 39 47128.0 40 47286.5 41 47491.0 42 47550.5 43 48558.5 44 48830.0 45 48652.0 46 51155.0 47 59599.5 48 92358.0 49 90439.0 50 55302.5 51 43317.0 52 34414.0 53 28223.0 54 23683.5 55 20120.5 56 16885.5 57 13631.5 58 10938.0 59 8814.5 60 6982.5 61 5294.0 62 3909.0 63 2723.5 64 1760.0 65 1151.5 66 723.0 67 416.0 68 236.0 69 132.5 70 67.5 71 27.5 72 14.0 73 11.0 74 6.5 75 6.5 76 4.5 77 3.0 78 2.5 79 1.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06551340439750046 2 0.0033952765529917787 3 0.03402993090612214 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.8582688102179305E-5 14-15 0.0015047248359849929 16-17 0.0 18-19 0.0 20-21 0.011459058366347254 22-23 0.0 24-25 0.0 26-27 0.005362993646202923 28-29 0.011767719871164687 30-31 0.009066931704012137 32-33 0.004552757196057158 34-35 0.0052472455818963855 36-37 0.019870084372622342 38-39 0.04274961841721467 40-41 0.004668505260363696 42-43 0.008526774070581626 44-45 0.007716537620435861 46-47 0.003511024617298317 48-49 7.716537620435862E-4 50-51 0.001041732578758841 52-53 5.401576334305103E-4 54-55 9.259845144523034E-4 56-57 0.010262995035179696 58-59 0.007292128051311888 60-61 0.0036267726816048545 62-63 0.004359843755546261 64-65 0.08569215027494025 66-67 0.03869843616648584 68-69 0.02874410263612358 70-71 0.019059847922476576 72-73 1.5433075240871722E-4 74-75 2.3149612861307584E-4 76-77 3.0866150481743444E-4 78-79 3.4724419291961374E-4 80-81 3.858268810217931E-4 82-83 3.0866150481743444E-4 84-85 4.2440956912397234E-4 86-87 0.00277795354335691 88-89 1.5433075240871722E-4 90-91 7.330710739414068E-4 92-93 2.7007881671525514E-4 94-95 0.0015818902121893515 96-97 0.0037811034340135717 98-99 0.005633072462918178 100-101 0.007909451060946758 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1295918.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.2951953757752 #Duplication Level Percentage of deduplicated Percentage of total 1 84.61107269686585 52.70863304605178 2 9.962857704562685 12.412763344135598 3 2.3024326929221433 4.302914833354715 4 0.9591220877567386 2.3899479138410977 5 0.5127373250610807 1.5970535920566193 6 0.3178292186553531 1.1879539963359118 7 0.20581562173741255 0.8974927057263132 8 0.15458258398092256 0.7703801816627001 9 0.11918994861063026 0.6682465021975037 >10 0.7618751843504057 8.946609395264305 >50 0.05900247998217761 2.5310527935523566 >100 0.02653796997619012 3.062332161004474 >500 0.003100216758213692 1.327720558816967 >1k 0.0033482340988707873 4.4679917356314025 >5k 2.4801734065709537E-4 0.9950758432606825 >10k+ 2.4801734065709537E-4 1.7338313971075814 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 11768 0.908082147172892 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10676 0.8238175563577326 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6734 0.5196316433601509 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6147 0.47433556752819234 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4506 0.3477071851768399 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4178 0.3223969417818103 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4156 0.3206993035053144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.26336542898547594 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2884 0.2225449449733702 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2599 0.200552812755128 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2564 0.19785202458797546 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2429 0.18743469880038707 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2254 0.1739307579646243 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2177 0.1679890239968887 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2070 0.15973232874302232 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2033 0.15687720982346104 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2012 0.15525673692316952 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1817 0.14020948856331958 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1796 0.13858901566302806 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1784 0.13766303114857575 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1744 0.1345764161004014 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1636 0.1262425554703307 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1632 0.12593389396551327 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1580 0.1219212944028866 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1533 0.11829452172128176 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1320 0.10185829658975337 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 7.716537620435861E-5 0.0 0.0 0.0 4 0.0 7.716537620435861E-5 0.0 0.0 0.0 5 0.0 2.3149612861307582E-4 0.0 0.0 0.0 6 0.0 2.3149612861307582E-4 0.0 0.0 0.0 7 0.0 2.3149612861307582E-4 0.0 0.0 0.0 8 0.0 2.3149612861307582E-4 0.0 7.716537620435861E-5 0.0 9 0.0 2.3149612861307582E-4 0.0 3.0866150481743444E-4 0.0 10-11 0.0 2.3149612861307582E-4 0.0 3.4724419291961374E-4 0.0 12-13 0.0 2.3149612861307582E-4 0.0 5.01574945328331E-4 0.0 14-15 0.0 2.3149612861307582E-4 0.0 7.330710739414068E-4 0.0 16-17 0.0 2.3149612861307582E-4 0.0 0.0011574806430653792 0.0 18-19 0.0 2.3149612861307582E-4 0.0 0.0013503940835762757 0.0 20-21 0.0 2.3149612861307582E-4 0.0 0.002044882469415503 0.0 22-23 0.0 2.3149612861307582E-4 0.0 0.003433859241093958 0.0 24-25 0.0 2.3149612861307582E-4 0.0 0.004629922572261517 0.0 26-27 0.0 2.3149612861307582E-4 0.0 0.005748820527224717 0.0 28-29 0.0 2.3149612861307582E-4 0.0 0.009915750842260082 0.0 30-31 0.0 2.3149612861307582E-4 0.0 0.017863784591309016 0.0 32-33 0.0 2.3149612861307582E-4 0.0 0.030904733169845624 0.0 34-35 0.0 2.3149612861307582E-4 0.0 0.041977964655171084 0.0 36-37 0.0 2.3149612861307582E-4 0.0 0.049617336899402585 0.0 38-39 0.0 2.7007881671525514E-4 0.0 0.05887718204392562 0.0 40-41 0.0 3.0866150481743444E-4 0.0 0.07218820943917748 0.0 42-43 0.0 3.0866150481743444E-4 0.0 0.09688112982457223 0.0 44-45 0.0 3.0866150481743444E-4 0.0 0.13002365890434425 0.0 46-47 0.0 3.0866150481743444E-4 0.0 0.16065831325747462 0.0 48-49 0.0 3.0866150481743444E-4 0.0 0.22385675636884433 0.0 50-51 0.0 3.0866150481743444E-4 0.0 0.2848174035702876 0.0 52-53 0.0 3.0866150481743444E-4 0.0 0.3430772626045784 0.0 54-55 0.0 3.0866150481743444E-4 0.0 0.4626835957213342 0.0 56-57 0.0 3.0866150481743444E-4 0.0 0.5875371744199864 0.0 58-59 0.0 3.0866150481743444E-4 0.0 0.7557576945454882 0.0 60-61 0.0 3.0866150481743444E-4 0.0 0.9531467268762375 0.0 62-63 0.0 3.0866150481743444E-4 0.0 1.2294759390640457 0.0 64-65 0.0 3.0866150481743444E-4 0.0 1.396307482417869 0.0 66-67 0.0 3.0866150481743444E-4 0.0 1.7694792417421472 0.0 68-69 0.0 3.0866150481743444E-4 0.0 2.206929759444656 0.0 70-71 0.0 3.0866150481743444E-4 0.0 2.3386510566254963 0.0 72-73 0.0 3.0866150481743444E-4 0.0 2.6045243603376136 0.0 74-75 0.0 3.0866150481743444E-4 0.0 2.9766929697712357 0.0 76-77 0.0 3.0866150481743444E-4 0.0 3.114587497048424 0.0 78-79 0.0 3.0866150481743444E-4 0.0 3.2318788688790496 0.0 80-81 0.0 3.0866150481743444E-4 0.0 3.503385245054085 0.0 82-83 0.0 3.0866150481743444E-4 0.0 3.9341609577149175 0.0 84-85 0.0 3.0866150481743444E-4 0.0 4.62112571937422 0.0 86-87 0.0 3.0866150481743444E-4 0.0 5.821355980856813 0.0 88-89 0.0 3.0866150481743444E-4 0.0 6.817097995397857 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1370 0.0 38.831627 2 ACCTGGG 1460 0.0 35.78584 3 TAACGCA 230 0.0 35.09877 4 GTACACG 245 0.0 34.91504 1 TATAACG 225 0.0 33.777355 2 ATAACGC 235 0.0 32.33877 3 ACACGGG 240 0.0 31.665047 3 TACACGG 250 0.0 30.399616 2 GTACCTG 1870 0.0 29.733795 1 GTACCCG 215 1.8189894E-12 28.734987 1 GTCGAGC 85 6.418224E-4 27.933279 8 ACAACGC 120 5.3845124E-6 27.706915 3 TACCCGG 230 5.456968E-12 26.847488 2 CCTGGGG 1075 0.0 22.086779 4 GTATAAC 495 0.0 22.081507 1 GGCATCG 285 8.367351E-11 21.660542 7 CCAACGG 165 1.8189894E-12 21.619423 62-63 GTACCGG 110 0.002869404 21.601475 1 GCATCGT 270 1.0059011E-9 21.105145 8 TAGCGTA 115 0.0037296347 20.646336 9 >>END_MODULE