##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003834_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1392309 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.679817483044353 31.0 31.0 34.0 30.0 34.0 2 31.8656181925133 31.0 31.0 34.0 30.0 34.0 3 31.921167643102212 31.0 31.0 34.0 30.0 34.0 4 35.6880168123599 37.0 35.0 37.0 33.0 37.0 5 35.54131302749605 37.0 35.0 37.0 33.0 37.0 6 35.58935121442151 37.0 35.0 37.0 33.0 37.0 7 35.535177176905414 37.0 35.0 37.0 33.0 37.0 8 35.534833862310734 37.0 35.0 37.0 33.0 37.0 9 37.11749475152427 39.0 37.0 39.0 33.0 39.0 10-11 37.03374897382693 39.0 37.0 39.0 33.0 39.0 12-13 36.98933031388866 39.0 37.0 39.0 33.0 39.0 14-15 38.107573462500056 40.0 37.0 41.0 33.0 41.0 16-17 38.10667459594099 40.0 37.0 41.0 33.0 41.0 18-19 38.16207178147954 40.0 37.0 41.0 33.0 41.0 20-21 38.10720536892313 40.0 37.0 41.0 33.0 41.0 22-23 38.03136300921706 40.0 37.0 41.0 33.0 41.0 24-25 37.912801324993225 40.0 37.0 41.0 33.0 41.0 26-27 37.71203303289715 39.5 37.0 41.0 32.0 41.0 28-29 37.536621181074025 39.0 36.5 41.0 32.0 41.0 30-31 37.33212419082258 39.0 36.0 41.0 31.5 41.0 32-33 37.08217141453514 39.0 36.0 40.5 31.0 41.0 34-35 37.0392955155788 39.0 36.0 41.0 31.0 41.0 36-37 36.8582735585276 39.0 35.5 41.0 30.5 41.0 38-39 36.616556382239864 39.0 35.0 40.0 30.0 41.0 40-41 36.62386223173161 39.0 35.0 41.0 30.0 41.0 42-43 36.508098058692426 39.0 35.0 41.0 30.0 41.0 44-45 36.22659984242004 39.0 35.0 41.0 30.0 41.0 46-47 36.06084317489868 39.0 35.0 41.0 29.5 41.0 48-49 35.824351850056274 39.0 35.0 41.0 28.0 41.0 50-51 35.59778037777534 39.0 35.0 40.0 27.5 41.0 52-53 35.358754414429555 39.0 35.0 40.0 27.0 41.0 54-55 35.16073227997521 38.0 34.0 40.0 26.0 41.0 56-57 34.92302498942404 38.0 34.0 40.0 26.0 41.0 58-59 34.66754219070623 38.0 34.0 40.0 25.5 41.0 60-61 34.32155900737552 38.0 33.5 40.0 24.5 41.0 62-63 34.047356944471375 37.5 33.0 40.0 23.5 41.0 64-65 33.583034728641415 37.0 32.5 39.5 22.5 41.0 66-67 33.226686030184396 36.0 32.0 39.0 22.0 41.0 68-69 32.956354516131114 36.0 32.0 39.0 22.0 40.5 70-71 32.52419075076007 35.5 31.5 39.0 21.0 40.0 72-73 32.12867043163551 35.0 31.0 38.0 20.0 40.0 74-75 31.73630602114904 35.0 31.0 37.0 18.5 39.5 76-77 30.657557338205812 34.0 29.5 36.0 18.0 39.0 78-79 31.06364248166176 34.5 31.0 36.0 18.0 39.0 80-81 30.877184949605297 34.5 31.0 36.0 17.0 38.5 82-83 30.577164623657538 34.0 31.0 36.0 13.0 37.0 84-85 30.22250376891911 34.0 30.0 35.0 9.0 37.0 86-87 29.883183618004338 34.0 30.0 35.0 7.0 36.5 88-89 29.5361633085759 34.0 29.5 35.0 2.0 36.0 90-91 29.220429875839343 34.0 29.0 35.0 2.0 36.0 92-93 28.997218649021157 34.0 29.0 35.0 2.0 35.5 94-95 28.684871318076663 34.0 29.0 35.0 2.0 35.0 96-97 28.319071772142536 34.0 29.0 35.0 2.0 35.0 98-99 27.934942602540097 34.0 29.0 35.0 2.0 35.0 100-101 27.037550572466316 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.04534880722066603 1101 2 0.10111951698606703 1101 3 0.08293764810131776 1101 4 -0.08221958508206484 1101 5 0.12113181234479953 1101 6 0.08864542986029988 1101 7 -0.0014963519274502346 1101 8 0.025124873934061043 1101 9 0.1360631662499685 1101 10-11 0.17854814596241653 1101 12-13 0.1603504890513605 1101 14-15 0.2702906126931097 1101 16-17 0.21235846145847148 1101 18-19 0.09071084813118802 1101 20-21 -0.04306181013679122 1101 22-23 -0.0664640377720076 1101 24-25 -0.03872408384036419 1101 26-27 0.05884702879772874 1101 28-29 0.14113668650634992 1101 30-31 0.20456804014661856 1101 32-33 0.22772662451246362 1101 34-35 0.1622858787281345 1101 36-37 0.2592920357587474 1101 38-39 0.21146819704416941 1101 40-41 0.3171737731959041 1101 42-43 0.3074411756764519 1101 44-45 0.1955345583055248 1101 46-47 0.22284434177726808 1101 48-49 0.33173481338289434 1101 50-51 0.26696974165329834 1101 52-53 0.2130752050434097 1101 54-55 0.3479526615916697 1101 56-57 0.27131448720187734 1101 58-59 0.17207792648010667 1101 60-61 0.13880071625173684 1101 62-63 0.10060792713669287 1101 64-65 0.10863697546506046 1101 66-67 -0.14219814657750618 1101 68-69 0.19772565521352448 1101 70-71 0.4508324558880119 1101 72-73 0.3507019320099438 1101 74-75 0.24285843920074868 1101 76-77 0.1815600832375175 1101 78-79 0.10867776497035564 1101 80-81 0.09780953342641752 1101 82-83 0.34344389166581735 1101 84-85 0.06867153823753824 1101 86-87 -0.007141240236780533 1101 88-89 -0.06119582382004296 1101 90-91 -0.03583825240367133 1101 92-93 -0.07557372575385202 1101 94-95 0.12219060504597934 1101 96-97 0.09397781896888446 1101 98-99 -0.12826282549275447 1101 100-101 -0.2359885915065547 1102 1 0.1104944618125252 1102 2 0.13132066165887935 1102 3 0.16927800282960703 1102 4 0.06621638791487783 1102 5 0.0946424597063853 1102 6 0.048504200088785865 1102 7 0.08410039146838244 1102 8 0.015134671812106149 1102 9 0.06452299494998215 1102 10-11 0.1331580976997131 1102 12-13 -0.010915980655404667 1102 14-15 -0.06311977556347159 1102 16-17 -0.021880674423805146 1102 18-19 0.031234157558706954 1102 20-21 -0.05586608311418928 1102 22-23 0.05903901184885996 1102 24-25 0.08155771165210979 1102 26-27 0.02180271783162624 1102 28-29 0.008823673120090803 1102 30-31 0.06075379638743783 1102 32-33 0.0706363812945483 1102 34-35 0.2752349272564416 1102 36-37 0.12033287862684716 1102 38-39 0.16403180807592577 1102 40-41 0.14145315304786266 1102 42-43 0.29496830151528997 1102 44-45 0.4150474333687981 1102 46-47 0.14085915636933777 1102 48-49 0.23952230968676247 1102 50-51 0.4588675104434188 1102 52-53 0.37079034489496365 1102 54-55 0.3172293601828784 1102 56-57 0.334059434153005 1102 58-59 0.40140183530721885 1102 60-61 0.3286089184037664 1102 62-63 0.29544631226596607 1102 64-65 0.26359849812045866 1102 66-67 0.3321300347258358 1102 68-69 0.2209658585884 1102 70-71 0.20759950695224205 1102 72-73 0.14659440343070784 1102 74-75 0.09244159085987036 1102 76-77 0.10967755002246093 1102 78-79 0.29972009881989337 1102 80-81 0.34356963325952705 1102 82-83 0.13055178860516747 1102 84-85 0.018666651827768277 1102 86-87 1.8712148784416627E-4 1102 88-89 0.1043112602211913 1102 90-91 0.14632710370283775 1102 92-93 0.17116553542098956 1102 94-95 0.189691970734863 1102 96-97 0.1917902735503354 1102 98-99 0.07380337408325488 1102 100-101 0.23948695639780126 1103 1 0.0023459112531654114 1103 2 0.02911543286275986 1103 3 0.024883414369561763 1103 4 0.008612682168404717 1103 5 -0.02059743650838186 1103 6 -0.02331468169033002 1103 7 0.03459367542493652 1103 8 0.010065672880642751 1103 9 0.10681257343652106 1103 10-11 0.05455612954028055 1103 12-13 -0.10886743880471528 1103 14-15 -0.06821054617018518 1103 16-17 -0.009932862857311875 1103 18-19 -0.052690121245916544 1103 20-21 -0.18771431172922348 1103 22-23 -0.010405029416411082 1103 24-25 -0.05175025595164584 1103 26-27 -0.17488639785131 1103 28-29 -0.09903873542159403 1103 30-31 -0.023490837067399184 1103 32-33 -0.09701035634086708 1103 34-35 -0.22493337124911505 1103 36-37 -0.1733276242366486 1103 38-39 -0.07147309435240601 1103 40-41 -0.2080793434237478 1103 42-43 0.08319176046082788 1103 44-45 0.17716838159091708 1103 46-47 0.15621371674047424 1103 48-49 0.08050268476780076 1103 50-51 0.0914404248206111 1103 52-53 0.31699218975069954 1103 54-55 0.1733185867285627 1103 56-57 -0.11465824873251762 1103 58-59 0.136670705129184 1103 60-61 0.09892431049436112 1103 62-63 -0.04906107420684691 1103 64-65 0.004788572448262585 1103 66-67 0.026955080308120216 1103 68-69 -0.09839904436521607 1103 70-71 -0.023699315165565338 1103 72-73 -0.03707801510521236 1103 74-75 -0.33163332029095827 1103 76-77 -0.23543633311824763 1103 78-79 -0.07301754765510182 1103 80-81 -0.03840507842128815 1103 82-83 -0.06144501268648028 1103 84-85 -0.19579886391211687 1103 86-87 -0.15773458895025172 1103 88-89 -0.03385213654719976 1103 90-91 0.029198601054986284 1103 92-93 0.23040833729045218 1103 94-95 -0.11367420672571527 1103 96-97 0.12414740643496103 1103 98-99 0.04084624147509075 1103 100-101 0.1378709451622342 1104 1 0.08681632991351051 1104 2 0.10631551440225451 1104 3 0.1183938018266062 1104 4 0.08672125489023585 1104 5 0.027276517563052494 1104 6 0.07008736097023416 1104 7 -0.015040279673769419 1104 8 -0.039284980639116895 1104 9 0.09094607990751769 1104 10-11 0.03365561574516818 1104 12-13 0.050831186099181025 1104 14-15 0.11581389320568292 1104 16-17 0.13216093644960125 1104 18-19 -0.03592476718315396 1104 20-21 0.08682939616678453 1104 22-23 0.03331249724042351 1104 24-25 0.21999374214791345 1104 26-27 0.024792259507876224 1104 28-29 0.1322188186183837 1104 30-31 0.011766566092418884 1104 32-33 0.18000281036083976 1104 34-35 0.38949237725291397 1104 36-37 0.16927427203805934 1104 38-39 0.4483636340520647 1104 40-41 0.5248118093809353 1104 42-43 0.31428479092095785 1104 44-45 0.5735682846423913 1104 46-47 0.5525686767652545 1104 48-49 0.47179433130374093 1104 50-51 0.6399893268464112 1104 52-53 0.5816636803022277 1104 54-55 0.4395518474575084 1104 56-57 0.6456288890547768 1104 58-59 0.6406782587678848 1104 60-61 0.5889124487455248 1104 62-63 0.4724228963811612 1104 64-65 0.5077231113247862 1104 66-67 0.6506595574361356 1104 68-69 0.6460424662983257 1104 70-71 0.6564391575098796 1104 72-73 0.5963181047064801 1104 74-75 0.2975353460728769 1104 76-77 0.21554647456893505 1104 78-79 0.249790958133719 1104 80-81 0.19706668692475304 1104 82-83 0.23818693602710894 1104 84-85 0.3449163080511397 1104 86-87 0.32079508388056155 1104 88-89 0.14285782523517554 1104 90-91 0.1839779709216458 1104 92-93 0.38070584407604713 1104 94-95 0.589855104955781 1104 96-97 0.36371540764200105 1104 98-99 0.507267380106672 1104 100-101 0.7968950233887853 1105 1 -0.041899671992524645 1105 2 -0.05862845391732918 1105 3 -0.10524851883896602 1105 4 -0.10969299379986097 1105 5 -0.10303277889134677 1105 6 -0.007065091174204952 1105 7 -0.024319370617838842 1105 8 -0.08890056749931574 1105 9 -0.1980662057253042 1105 10-11 -0.23973301099277222 1105 12-13 -0.12118666471912576 1105 14-15 -0.07882627379920848 1105 16-17 0.09918245233864553 1105 18-19 -0.20330849860992117 1105 20-21 -0.21211138359058168 1105 22-23 -0.133773786318649 1105 24-25 -0.12170257887085256 1105 26-27 -0.11607249000945075 1105 28-29 -0.059903647032058416 1105 30-31 -0.03882300221848567 1105 32-33 0.04924117610431722 1105 34-35 -0.055172700394301444 1105 36-37 0.12926802711267982 1105 38-39 0.10559862181379032 1105 40-41 0.24543550280542092 1105 42-43 0.5089254512058972 1105 44-45 0.4374422298863294 1105 46-47 0.38715263944171596 1105 48-49 0.24829284209127422 1105 50-51 0.4260944665285322 1105 52-53 0.43956375754988386 1105 54-55 0.425995771875165 1105 56-57 0.3730216179095862 1105 58-59 0.3887312824265834 1105 60-61 0.4956445375004179 1105 62-63 0.3242738804255225 1105 64-65 0.005226867652332601 1105 66-67 0.10344303807932675 1105 68-69 0.2177540395361035 1105 70-71 0.28637775400976295 1105 72-73 0.0211503648268625 1105 74-75 0.1306934827765609 1105 76-77 0.2626196960723952 1105 78-79 0.26755347930776807 1105 80-81 0.14579392575022965 1105 82-83 0.24459143750349455 1105 84-85 0.16027492757733341 1105 86-87 0.23251670030007787 1105 88-89 0.19807658013714757 1105 90-91 0.019866905959624148 1105 92-93 0.20403734898467718 1105 94-95 0.008098130222567335 1105 96-97 0.0585383349039148 1105 98-99 0.27845918416005233 1105 100-101 0.32087484626178764 1106 1 -0.111695458977902 1106 2 -0.0472371310938442 1106 3 0.03316429145323596 1106 4 -0.05002515960795506 1106 5 -0.09494110377464438 1106 6 -0.06269154077362771 1106 7 -0.18948369141967447 1106 8 0.00801123705873863 1106 9 -0.034805845900223176 1106 10-11 -0.025071111968230753 1106 12-13 0.03933035006345165 1106 14-15 0.12061125299810271 1106 16-17 -0.015241276625864941 1106 18-19 -0.08373745917318587 1106 20-21 0.0058813339018115585 1106 22-23 -0.07361230905359406 1106 24-25 -0.11596715883530351 1106 26-27 0.005176363351836244 1106 28-29 -0.25976547786261506 1106 30-31 -0.12351650457791408 1106 32-33 0.020070750922414504 1106 34-35 0.1433462431586392 1106 36-37 0.28487055792401605 1106 38-39 0.16906356361352692 1106 40-41 0.2930943241944135 1106 42-43 0.3054242749867697 1106 44-45 0.4143434236628707 1106 46-47 0.4484445338987868 1106 48-49 0.5042168770381537 1106 50-51 0.45513766411425394 1106 52-53 0.45467457914752885 1106 54-55 0.5117395380410201 1106 56-57 0.1985630031382115 1106 58-59 0.2316182419796533 1106 60-61 0.2731244259981551 1106 62-63 0.17319524486515547 1106 64-65 0.27119870200709784 1106 66-67 0.10161700400523443 1106 68-69 0.11246938687379782 1106 70-71 0.25119868752952357 1106 72-73 0.2606604495222662 1106 74-75 0.3251020207660851 1106 76-77 0.22730385992627333 1106 78-79 0.49667129587865233 1106 80-81 0.5499874109339409 1106 82-83 0.5186966140127005 1106 84-85 0.3502712125315277 1106 86-87 0.4406801317810185 1106 88-89 0.37828683646258554 1106 90-91 0.4065834543258333 1106 92-93 0.48882169184778235 1106 94-95 0.656753716213796 1106 96-97 0.5545408438321111 1106 98-99 0.4572565780299733 1106 100-101 0.5498132719220621 1107 1 -0.11388711379461469 1107 2 -0.1619016639866544 1107 3 -0.05867167702823295 1107 4 0.04868143553661497 1107 5 -0.03847587850037826 1107 6 0.04657676564505664 1107 7 -0.07541282947828876 1107 8 0.02284284256712965 1107 9 0.03994428824282181 1107 10-11 -0.09518412063629711 1107 12-13 -0.0633813730215067 1107 14-15 -0.03945229091242908 1107 16-17 -0.0476487492145381 1107 18-19 0.02544793667784262 1107 20-21 0.028021975254162612 1107 22-23 0.027883522734946098 1107 24-25 0.0642108016585965 1107 26-27 0.15767901101477833 1107 28-29 0.11283226665707957 1107 30-31 0.027695945147776513 1107 32-33 -0.05789920729379361 1107 34-35 -0.1108239237419042 1107 36-37 0.13416182900559903 1107 38-39 0.11078525630316705 1107 40-41 0.21864063443949533 1107 42-43 0.214534738456841 1107 44-45 0.22998473690368826 1107 46-47 0.008443626575591168 1107 48-49 0.08768513558771929 1107 50-51 0.002000515956197546 1107 52-53 -0.04251584765417249 1107 54-55 -0.011724152605573579 1107 56-57 -0.11396173315143443 1107 58-59 -0.25307341674793093 1107 60-61 -0.28693137437823424 1107 62-63 -0.16726174879766376 1107 64-65 -0.30538485072636945 1107 66-67 -0.25197583940485657 1107 68-69 -0.15930050344960023 1107 70-71 -0.25569618656373905 1107 72-73 -0.12827106791319665 1107 74-75 -0.10287411663393087 1107 76-77 -0.01815052909323711 1107 78-79 -0.1592568879262508 1107 80-81 0.054193557892137534 1107 82-83 0.10494354984148302 1107 84-85 0.2635189915451406 1107 86-87 0.13340418194660586 1107 88-89 0.015210510245481146 1107 90-91 0.06189215539882298 1107 92-93 0.1784882192872672 1107 94-95 0.2968557587860232 1107 96-97 0.3614530822140729 1107 98-99 0.1925145315781549 1107 100-101 0.09157595059042478 1108 1 0.06744525935216572 1108 2 0.0018520557836616547 1108 3 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-0.7515498101219862 2316 100-101 -1.122082445927525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 10.0 5 21.0 6 65.0 7 187.0 8 532.0 9 998.0 10 1625.0 11 2819.0 12 3967.0 13 5530.0 14 6594.0 15 8526.0 16 10701.0 17 13614.0 18 12352.0 19 8786.0 20 8003.0 21 8014.0 22 8430.0 23 9224.0 24 10745.0 25 12709.0 26 15743.0 27 19749.0 28 24681.0 29 30701.0 30 37954.0 31 46527.0 32 57333.0 33 71235.0 34 92234.0 35 122947.0 36 174212.0 37 250625.0 38 252791.0 39 62075.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90340311276441 20.1069114408753 15.257518872643436 20.732166573716864 2 14.597164431783819 22.55389324995708 39.25952581553651 23.589416502722585 3 21.018028653566894 26.48913689374871 30.774525787707237 21.71830866497716 4 11.909209809029461 16.976906706772706 40.83942573092611 30.274457753271722 5 12.031596434412188 38.91851593288559 35.20741444607483 13.842473186627394 6 27.63323371464237 38.950189936285696 17.858032951018778 15.558543398053162 7 25.644953814131778 33.77210087703232 22.366227611830418 18.21671769700548 8 24.046242608501416 33.3082670585337 20.86878702931605 21.77670330364883 9 23.665579982604438 19.583799285934372 23.71628711729939 33.034333614161795 10-11 24.029866933274153 26.05937331440075 28.742362507173336 21.16839724515176 12-13 26.91414046738188 24.092999470663482 28.263122625796427 20.72973743615821 14-15 22.358716711586908 26.099300968014756 29.277212060052467 22.26477026034587 16-17 19.844481361536843 29.504765106021726 29.402812163104596 21.247941369336836 18-19 19.12161739958587 30.37292727404621 30.79804842172248 19.70740690464545 20-21 21.0118108044528 27.53190641145129 29.39121471528904 22.06506806880687 22-23 20.159820844367164 27.638153599524244 29.967018815507192 22.235006740601403 24-25 21.710302813527743 27.64397127361814 30.078057385249966 20.567668527604148 26-27 21.48410368607159 26.215145222702102 31.918432225455216 20.382318865771083 28-29 20.058549746273656 28.645091637069154 31.346166485711485 19.950192130945705 30-31 20.64122685725573 28.120818144270654 30.096000861960604 21.14195413651301 32-33 20.570090537876148 28.00177412577618 30.452689380743912 20.975445955603757 34-35 21.994434843567966 26.677088765250666 30.63712709214112 20.691349299040247 36-37 21.91511631081157 26.537305520076202 31.867768737527353 19.67980943158488 38-39 21.361869815265454 27.27846279817669 31.616600635722243 19.74306675083561 40-41 20.35891455802239 28.05371687273362 31.183525944611702 20.403842624632286 42-43 20.041971624796805 28.920410507344496 29.414976033146306 21.622641834712393 44-45 20.797703402623217 27.682934231418894 29.30919985791725 22.21016250804064 46-47 21.721690625623992 28.12618064962844 29.074112115566113 21.07801660918145 48-49 21.903079852947354 28.101606669010526 29.27068740323948 20.724626074802636 50-51 20.53289549052795 28.428861856500898 30.694245879332506 20.343996773638644 52-53 20.82886080658825 29.452490902620237 28.644293798052356 21.074354492739154 54-55 20.065984626810142 28.443705136989745 29.620288531014282 21.87002170518583 56-57 21.7592634161461 27.78987132552985 28.7718011588496 21.67906409947445 58-59 22.492191296271756 27.525101521229224 28.761528993931535 21.221178188567492 60-61 21.137072361533647 27.66098427372884 30.78871259628027 20.41323076845724 62-63 21.35699001810776 27.93755445435746 29.67212102101606 21.033334506518724 64-65 20.526810276847478 29.38186089408084 29.72331066825674 20.368018160814934 66-67 20.597182754520276 27.77145895378956 28.925624288234175 22.705734003455994 68-69 20.32474210694771 28.953243559453973 29.016142989695055 21.70587134390326 70-71 21.49252316072771 28.513852257670347 28.333400978617746 21.6602236029842 72-73 20.925392837494055 27.361442096780447 29.95261099212386 21.760554073601636 74-75 20.66443774891277 29.519142716574315 29.05024401980888 20.76617551470403 76-77 21.176965748414226 27.832748749282217 28.744979980973934 22.245305521329627 78-79 20.803324567272245 28.13984614682955 29.47425539655024 21.582573889347962 80-81 21.54544390249508 28.465437380907403 28.604164766332516 21.384953950264993 82-83 20.90387161670316 27.838263247239546 30.02256339243101 21.23530174362628 84-85 20.671465206634927 28.369958022651034 29.057274715156268 21.90130205555777 86-87 20.91030575404571 28.43472761468605 28.42582114589622 22.229145485372023 88-89 21.141752911445863 27.82627244791287 28.35205466612416 22.679919974517105 90-91 20.91383421012089 26.426912045082286 29.535297208177703 23.123956536619122 92-93 20.928265769162813 27.71251434670252 29.332021840026545 22.027198044108122 94-95 20.18127152949559 27.784936320097454 29.41040425892567 22.623387891481286 96-97 18.806948513210816 28.018408122616428 30.811554135273266 22.363089228899486 98-99 19.98245911436485 28.98634942708393 28.405711634161197 22.62547982439002 100-101 19.235094320241668 28.104246287474982 29.31579350003286 23.344865892250485 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 184.0 1 149.0 2 208.5 3 310.5 4 246.5 5 210.5 6 321.5 7 587.5 8 922.0 9 1163.0 10 1166.0 11 1086.5 12 1172.0 13 1334.0 14 1521.0 15 1709.0 16 1839.0 17 2010.0 18 2220.0 19 2423.5 20 2697.5 21 3010.0 22 3479.0 23 4107.0 24 5007.0 25 6311.0 26 7898.0 27 10192.0 28 13474.5 29 17709.0 30 22125.5 31 26294.5 32 29978.0 33 33026.5 34 35545.0 35 37671.5 36 39820.5 37 42120.0 38 44250.0 39 44879.0 40 45359.5 41 46124.0 42 46518.0 43 47738.5 44 47946.0 45 48175.5 46 51469.0 47 69517.5 48 114476.5 49 111936.0 50 66981.5 51 47136.5 52 36382.0 53 30370.0 54 26531.0 55 22914.5 56 19109.0 57 15235.0 58 12138.5 59 9815.5 60 7736.0 61 5857.0 62 4302.0 63 3048.0 64 2019.5 65 1293.5 66 809.5 67 457.0 68 260.0 69 153.5 70 89.5 71 48.0 72 26.0 73 19.5 74 11.0 75 6.0 76 3.0 77 2.0 78 1.5 79 2.0 80 1.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06298889111540613 2 0.0047403270394718415 3 0.041657419437782846 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0019033131294849059 16-17 0.0 18-19 0.0 20-21 0.010881205249696727 22-23 0.0 24-25 0.0 26-27 0.0048839733133952306 28-29 0.011455790345390283 30-31 0.009624300352867071 32-33 0.005314912135165398 34-35 0.005386735272127093 36-37 0.01888948502092567 38-39 0.047654651374084346 40-41 0.005997231936301496 42-43 0.012784518379181634 44-45 0.011671259756275369 46-47 0.004812150176433536 48-49 0.0013287280337913494 50-51 0.002046959403408295 52-53 8.977892120211822E-4 54-55 0.0010773470544254186 56-57 0.01095302838665842 58-59 0.008080102908190638 60-61 0.006069055073263191 62-63 0.006320436052629122 64-65 0.08489494788872298 66-67 0.037635323767927956 68-69 0.02905245890100545 70-71 0.01748893385017263 72-73 2.154694108850837E-4 74-75 2.872925478467783E-4 76-77 6.104966641744038E-4 78-79 5.386735272127093E-4 80-81 7.182313696169457E-4 82-83 6.823198011360985E-4 84-85 7.54142938097793E-4 86-87 0.0044889460601059104 88-89 1.7955784240423643E-4 90-91 7.54142938097793E-4 92-93 2.154694108850837E-4 94-95 0.0023701635197359208 96-97 0.00700275585376522 98-99 0.009552477215905377 100-101 0.011743082893237061 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1392309.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.30149885048742 #Duplication Level Percentage of deduplicated Percentage of total 1 83.65364763058552 50.444403364348325 2 10.515457913704813 12.681957465912392 3 2.5500781109411026 4.6132059682670405 4 1.0118975016531397 2.440757441309916 5 0.564785469978698 1.7028705184346231 6 0.3540427284169911 1.2809584308396371 7 0.24184956221294257 1.020872376844225 8 0.1828315973207789 0.8820015484537382 9 0.12950679115965577 0.7028508256419869 >10 0.7243915742647459 7.896436578706665 >50 0.041795152521541895 1.7117630194358302 >100 0.02132842964450485 2.4010952882593775 >500 0.0033550150382371914 1.3737014368523541 >1k 0.004073946832145162 5.522695961728006 >5k 8.387537595592979E-4 3.884687778820217 >10k+ 1.1982196565132827E-4 1.4397419961455928 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19926 1.431147827098726 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8946 0.6425297832593195 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8731 0.6270878088125552 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8444 0.606474568504549 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8049 0.5781044294046795 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6701 0.4812868407803153 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6647 0.47740839138438373 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6246 0.4486073134627443 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4875 0.350137792688261 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4771 0.3426681864442448 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4730 0.33972343782881526 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4665 0.33505493392630514 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4079 0.2929665756667521 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3916 0.2812594043419959 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3231 0.23206055552323512 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2808 0.20167936858843832 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2657 0.19083407490722248 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2612 0.1876020337439462 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2484 0.1784086722128493 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2471 0.17747497143234728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2376 0.17065177342098628 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2246 0.161314765615966 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2119 0.1521932272218308 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1883 0.13524296689887086 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1826 0.13114904809205427 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1740 0.12497225831334854 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1631 0.11714353638452384 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1597 0.11470154972782623 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1596 0.11462972659086453 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1534 0.11017669209923947 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1529 0.10981757641443099 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1512 0.1085965830860822 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1452 0.1042871948683805 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0773470544254185E-4 0.0 12-13 0.0 7.182313696169457E-5 0.0 2.872925478467783E-4 0.0 14-15 0.0 7.182313696169457E-5 0.0 4.6685039025101465E-4 0.0 16-17 0.0 7.182313696169457E-5 0.0 6.464082326552511E-4 0.0 18-19 0.0 7.182313696169457E-5 0.0 8.97789212021182E-4 0.0 20-21 0.0 7.182313696169457E-5 0.0 0.0016878437185998223 0.0 22-23 0.0 7.182313696169457E-5 0.0 0.003088394889352866 0.0 24-25 0.0 7.182313696169457E-5 0.0 0.004345299786182521 0.0 26-27 0.0 7.182313696169457E-5 0.0 0.00581767409389726 0.0 28-29 0.0 7.182313696169457E-5 0.0 0.009983416037675545 0.0 30-31 0.0 7.182313696169457E-5 0.0 0.01720164130232585 0.0 32-33 0.0 7.182313696169457E-5 0.0 0.03142262242074137 0.0 34-35 0.0 7.182313696169457E-5 0.0 0.044314875505365545 0.0 36-37 0.0 7.182313696169457E-5 0.0 0.054262379974560244 0.0 38-39 0.0 7.182313696169457E-5 0.0 0.06668778266893341 0.0 40-41 0.0 7.182313696169457E-5 0.0 0.07825130771976622 0.0 42-43 0.0 7.182313696169457E-5 0.0 0.11477337286478792 0.0 44-45 0.0 7.182313696169457E-5 0.0 0.1507567644825969 0.0 46-47 0.0 7.182313696169457E-5 0.0 0.17661309378880694 0.0 48-49 0.0 7.182313696169457E-5 0.0 0.20706610386056543 0.0 50-51 0.0 7.182313696169457E-5 0.0 0.244090930964319 0.0 52-53 0.0 7.182313696169457E-5 0.0 0.32399417083420423 0.0 54-55 0.0 7.182313696169457E-5 0.0 0.37157699907132685 0.0 56-57 3.5911568480847285E-5 7.182313696169457E-5 0.0 0.5024028430470535 0.0 58-59 7.182313696169457E-5 7.182313696169457E-5 0.0 0.6628557310194791 0.0 60-61 7.182313696169457E-5 7.182313696169457E-5 0.0 0.7848114175804365 0.0 62-63 7.182313696169457E-5 7.182313696169457E-5 0.0 0.9881427183189938 0.0 64-65 1.4364627392338914E-4 7.182313696169457E-5 0.0 1.0724630811120233 0.0 66-67 2.154694108850837E-4 7.182313696169457E-5 0.0 1.4424599711701926 0.0 68-69 2.154694108850837E-4 7.182313696169457E-5 0.0 1.7886474913255606 0.0 70-71 2.154694108850837E-4 7.182313696169457E-5 0.0 1.8941915910907707 0.0 72-73 2.154694108850837E-4 7.182313696169457E-5 0.0 2.1435615226217744 0.0 74-75 2.154694108850837E-4 7.182313696169457E-5 0.0 2.3459950341483102 0.0 76-77 2.154694108850837E-4 7.182313696169457E-5 0.0 2.4260778318606 0.0 78-79 2.154694108850837E-4 7.182313696169457E-5 0.0 2.520130229711939 0.0 80-81 2.154694108850837E-4 7.182313696169457E-5 0.0 2.72066042810899 0.0 82-83 2.154694108850837E-4 7.182313696169457E-5 0.0 2.8698729951469106 0.0 84-85 2.154694108850837E-4 7.182313696169457E-5 0.0 3.2024859424165184 0.0 86-87 2.154694108850837E-4 7.182313696169457E-5 0.0 4.130584518235535 0.0 88-89 2.154694108850837E-4 7.182313696169457E-5 0.0 5.4645915525935695 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1615 0.0 39.706856 2 ATAACGC 240 0.0 37.60374 3 ACCTGGG 1665 0.0 37.37195 3 GTACCCG 155 3.6379788E-11 33.738384 1 GTACCTG 2005 0.0 32.483997 1 TATAACG 255 0.0 31.667444 2 TAACGCA 275 0.0 31.080507 4 GTACACG 210 1.8189894E-12 29.429802 1 TACACGG 205 3.092282E-11 27.805563 2 ACGGGTC 70 0.008405875 27.133776 5 ACACGGG 230 1.3460522E-10 24.782328 3 AGTACGC 410 0.0 24.329866 2 TACGCAG 380 0.0 23.742054 4 GAGTACG 495 0.0 23.049917 1 GTACGCA 400 0.0 22.562244 3 CCTGGGG 1245 0.0 22.121113 4 ATAAGCG 130 2.9421668E-4 21.922827 3 GTATAAC 480 0.0 21.789371 1 TACTCCG 205 6.85337E-7 20.846684 5 GAGTACT 5375 0.0 20.32097 12-13 >>END_MODULE