##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003833_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2725692 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.861055100869798 31.0 31.0 34.0 28.0 34.0 2 30.613646736315037 31.0 31.0 34.0 27.0 34.0 3 30.765199442930456 31.0 31.0 34.0 27.0 34.0 4 34.18184629811439 37.0 35.0 37.0 30.0 37.0 5 34.116740262656236 37.0 35.0 37.0 30.0 37.0 6 34.22831009519784 37.0 35.0 37.0 30.0 37.0 7 34.187998130383036 37.0 35.0 37.0 30.0 37.0 8 34.248473782070754 37.0 35.0 37.0 30.0 37.0 9 35.710520851218696 39.0 35.0 39.0 30.0 39.0 10-11 35.63009466953713 39.0 35.0 39.0 30.0 39.0 12-13 35.64471884570964 39.0 35.0 39.0 30.0 39.0 14-15 36.6109072485079 39.0 36.0 41.0 30.0 41.0 16-17 36.58661195028638 39.0 36.0 41.0 30.0 41.0 18-19 36.50719927269846 39.5 36.0 41.0 29.5 41.0 20-21 36.433275293026504 39.0 36.0 41.0 29.5 41.0 22-23 36.25024342442212 39.0 36.0 41.0 27.5 41.0 24-25 36.17220489328948 39.0 36.0 41.0 27.0 41.0 26-27 36.04410494656036 39.0 36.0 41.0 27.5 41.0 28-29 35.88803907411402 39.0 35.5 40.5 27.0 41.0 30-31 35.637659170588606 39.0 35.0 40.0 27.0 41.0 32-33 35.40917682555475 38.0 35.0 40.0 26.0 41.0 34-35 35.34175449757346 38.0 35.0 40.0 25.5 41.0 36-37 35.334747286193746 38.0 35.0 40.0 25.5 41.0 38-39 35.27821283549278 38.5 35.0 40.0 25.0 41.0 40-41 35.40334050949264 39.0 35.0 41.0 25.5 41.0 42-43 35.2955904408862 39.0 35.0 41.0 25.0 41.0 44-45 35.09924672340088 39.0 35.0 41.0 24.5 41.0 46-47 34.86075829550808 39.0 34.5 40.0 24.0 41.0 48-49 34.59011161202366 38.5 34.0 40.0 23.0 41.0 50-51 33.79228155639008 37.5 33.5 39.5 21.5 40.5 52-53 33.927596735067645 38.0 33.5 40.0 22.0 40.5 54-55 34.3029504067224 38.0 34.0 40.0 22.0 41.0 56-57 34.044799265654376 38.0 33.5 40.0 20.0 41.0 58-59 33.84046876903186 38.0 33.0 40.0 19.0 41.0 60-61 33.390140742240874 38.0 33.0 40.0 15.5 41.0 62-63 33.26928501092567 37.5 33.0 40.0 17.0 41.0 64-65 32.961992771010074 37.0 32.0 40.0 13.0 41.0 66-67 32.550457645251186 36.5 31.5 39.0 12.5 41.0 68-69 32.217768185106756 36.0 31.0 39.0 11.0 41.0 70-71 31.77701882677867 35.5 31.0 39.0 8.0 40.5 72-73 31.378156446142853 35.0 31.0 38.0 7.0 40.0 74-75 30.960785921520113 35.0 30.0 37.0 6.5 40.0 76-77 30.546726849548666 35.0 30.0 37.0 2.0 39.0 78-79 29.992403030129594 34.0 29.0 36.0 2.0 39.0 80-81 29.55504510414236 34.0 29.0 36.0 2.0 38.5 82-83 29.207894912557983 34.0 29.0 35.5 2.0 37.0 84-85 28.951859747909886 34.0 29.0 35.0 2.0 37.0 86-87 28.790967578141625 34.0 29.0 35.0 2.0 36.5 88-89 28.40768197580651 34.0 28.0 35.0 2.0 36.0 90-91 28.11487248008946 34.0 27.0 35.0 2.0 36.0 92-93 27.903341977009873 34.0 27.0 35.0 2.0 35.5 94-95 27.59309103889948 34.0 26.0 35.0 2.0 35.0 96-97 27.361156726438644 34.0 26.0 35.0 2.0 35.0 98-99 27.126056612412555 34.0 25.0 35.0 2.0 35.0 100-101 26.816404237896286 33.0 25.0 35.0 2.0 35.0 102-103 26.554344731539736 33.0 25.0 35.0 2.0 35.0 104-105 26.241095655708715 33.0 24.0 35.0 2.0 35.0 106-107 25.84207753480584 32.5 21.5 35.0 2.0 35.0 108-109 25.605615931660658 32.0 20.0 35.0 2.0 35.0 110-111 25.31358697167545 32.0 19.0 35.0 2.0 35.0 112-113 24.80113637197453 32.0 17.5 34.5 2.0 35.0 114-115 24.333713787177714 32.0 8.5 34.0 2.0 35.0 116-117 23.749607989457356 31.5 2.0 34.0 2.0 35.0 118-119 23.04658156534194 31.0 2.0 34.0 2.0 35.0 120-121 21.503382994116723 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6083282358221567 1101 2 0.7672590987036294 1101 3 0.8116994632244072 1101 4 1.0058160647075454 1101 5 1.0452533726985536 1101 6 1.063780195441204 1101 7 0.9267719275219619 1101 8 0.947709692463043 1101 9 1.0945776789837112 1101 10-11 1.0848192407838937 1101 12-13 0.8369480593708474 1101 14-15 1.0314721880253828 1101 16-17 1.081172524424133 1101 18-19 1.1549864437268553 1101 20-21 1.161486768304762 1101 22-23 1.2298531045679937 1101 24-25 1.2066456789596245 1101 26-27 1.1207488796363378 1101 28-29 1.1441088149038023 1101 30-31 1.1199863685531426 1101 32-33 1.258867013832294 1101 34-35 1.2469665106841887 1101 36-37 1.090722595559825 1101 38-39 1.1030540375550189 1101 40-41 1.225404427869769 1101 42-43 1.20512640134978 1101 44-45 1.1435770150868976 1101 46-47 1.3062028101596113 1101 48-49 1.2879126106373633 1101 50-51 1.0928349867792235 1101 52-53 1.0615423590111703 1101 54-55 1.233806526016501 1101 56-57 1.0962863344664697 1101 58-59 1.1097260166541574 1101 60-61 1.1547704515199513 1101 62-63 1.1065145875214668 1101 64-65 1.0150561062445291 1101 66-67 0.8862991551962764 1101 68-69 1.0067531175156361 1101 70-71 0.8230678681528616 1101 72-73 0.6647713406982234 1101 74-75 0.7454509850205007 1101 76-77 0.867974116368238 1101 78-79 1.064874197886919 1101 80-81 0.94950358504029 1101 82-83 0.9925664066799449 1101 84-85 0.9224545517986904 1101 86-87 0.9233315973888772 1101 88-89 1.0401399651952232 1101 90-91 0.8352327717525796 1101 92-93 0.8114148159324017 1101 94-95 0.8723514036650535 1101 96-97 0.8220526705138269 1101 98-99 1.0244768990122886 1101 100-101 1.0922114840420285 1101 102-103 1.0001682215138068 1101 104-105 0.7579774077107757 1101 106-107 0.8710157945458583 1101 108-109 0.7577950648404581 1101 110-111 0.6242834123692518 1101 112-113 0.5218227954399559 1101 114-115 0.37610452838486097 1101 116-117 0.5502880130551553 1101 118-119 0.5010065072315406 1101 120-121 0.3618705047598709 1102 1 0.6031754938273117 1102 2 0.6931109825259796 1102 3 0.7681421118291176 1102 4 1.113637058556563 1102 5 1.2119681422152908 1102 6 1.1575834009445174 1102 7 1.1426813494139694 1102 8 0.8992776247201064 1102 9 1.0566894029280718 1102 10-11 1.0032319526460611 1102 12-13 0.7063104274079564 1102 14-15 0.9625191796554944 1102 16-17 0.9508301292199448 1102 18-19 1.1034113622388588 1102 20-21 1.1323999898806107 1102 22-23 1.1531815746590581 1102 24-25 1.1297278977631606 1102 26-27 1.0924723473840388 1102 28-29 1.1050501832665987 1102 30-31 1.0432049523169766 1102 32-33 1.1438372121307836 1102 34-35 1.133870973128218 1102 36-37 1.0009110439913584 1102 38-39 1.0996581528641727 1102 40-41 1.1434543824589198 1102 42-43 1.212296815184338 1102 44-45 1.1194496846362014 1102 46-47 0.9867195671625453 1102 48-49 0.8231397840697525 1102 50-51 0.6297660602494872 1102 52-53 0.781103184403122 1102 54-55 0.8047163053700501 1102 56-57 0.9114773910433627 1102 58-59 0.8666071514224498 1102 60-61 0.8932714431447621 1102 62-63 0.7413940965016934 1102 64-65 0.807292835815602 1102 66-67 0.9019784777669386 1102 68-69 0.9062825922060327 1102 70-71 0.6493693591646945 1102 72-73 0.5890318586732448 1102 74-75 0.6466168392344436 1102 76-77 0.7289493773813618 1102 78-79 0.9125505654421211 1102 80-81 0.7918709866655504 1102 82-83 0.7040486011231408 1102 84-85 0.5208398847542632 1102 86-87 0.6763428799344275 1102 88-89 0.7933988229599507 1102 90-91 0.7285410916038728 1102 92-93 0.7774797997936744 1102 94-95 0.7923051719717158 1102 96-97 0.8835175214523616 1102 98-99 0.9935379402050977 1102 100-101 0.9893208116700976 1102 102-103 0.8878034942304929 1102 104-105 0.8668019417530317 1102 106-107 0.7174542860051751 1102 108-109 0.6593845771834737 1102 110-111 0.5999138024259949 1102 112-113 0.5750554393579463 1102 114-115 0.6697088793538448 1102 116-117 0.6570286786750295 1102 118-119 0.39499532795266035 1102 120-121 0.35935503461849905 1103 1 0.6485141502693921 1103 2 0.9384522552121233 1103 3 0.9062115483683506 1103 4 1.0577202448802012 1103 5 1.128044151498976 1103 6 1.1683992951390607 1103 7 1.1150128622300812 1103 8 1.0534022685589974 1103 9 1.1306401006618785 1103 10-11 1.1164096042911709 1103 12-13 1.0351181324742171 1103 14-15 1.2025116551100794 1103 16-17 1.200005408045449 1103 18-19 1.2977254960850502 1103 20-21 1.2586062525717026 1103 22-23 1.4436976330655682 1103 24-25 1.2620464317337365 1103 26-27 1.3456800545546699 1103 28-29 1.2885130894047236 1103 30-31 1.213958813089846 1103 32-33 1.2771000111450803 1103 34-35 1.4420521531481327 1103 36-37 1.1424283062967007 1103 38-39 1.0161254612594846 1103 40-41 1.2672635115297552 1103 42-43 1.3113906136479514 1103 44-45 1.3800787093331266 1103 46-47 1.3821421780234004 1103 48-49 1.3761386324958096 1103 50-51 1.2255596943305065 1103 52-53 1.1739030105935129 1103 54-55 1.1082196694475712 1103 56-57 1.2123735399463271 1103 58-59 1.188227934524015 1103 60-61 1.3726469087188988 1103 62-63 1.4262652955363038 1103 64-65 1.3353569588200216 1103 66-67 1.2466816428392846 1103 68-69 1.2989319843629659 1103 70-71 0.9835080169717685 1103 72-73 0.882029723164031 1103 74-75 1.05877424100834 1103 76-77 1.0336861441274223 1103 78-79 1.0380569828470847 1103 80-81 0.8849216168177918 1103 82-83 1.066455776001849 1103 84-85 1.037126004029684 1103 86-87 1.0382541770326839 1103 88-89 1.0566689033795953 1103 90-91 1.2691383033417658 1103 92-93 1.1301807092086413 1103 94-95 1.1402689022978123 1103 96-97 0.9426712262174668 1103 98-99 0.8465546473406071 1103 100-101 0.915621714146333 1103 102-103 0.7883929027360992 1103 104-105 0.9947795012039791 1103 106-107 1.0240929484333385 1103 108-109 1.0628491525990533 1103 110-111 0.893022430727811 1103 112-113 1.0769729045359533 1103 114-115 0.96716274779061 1103 116-117 0.9527956384416036 1103 118-119 0.8475404997524478 1103 120-121 0.8137118132428789 1104 1 0.8862044949014241 1104 2 0.9469443436412917 1104 3 1.030051197419322 1104 4 1.0152510443358693 1104 5 1.2288408646909872 1104 6 1.247708646152681 1104 7 1.194941459386449 1104 8 1.099958356795483 1104 9 1.1869548238581729 1104 10-11 1.2529815698619018 1104 12-13 1.1234409703266977 1104 14-15 1.4051095603201205 1104 16-17 1.3292680111071604 1104 18-19 1.2965472862141212 1104 20-21 1.4034457357605064 1104 22-23 1.4722058780029386 1104 24-25 1.278706137298279 1104 26-27 1.252401263235548 1104 28-29 1.3128317407427303 1104 30-31 1.454398063195768 1104 32-33 1.477515980333031 1104 34-35 1.5453424097582626 1104 36-37 1.3611868252077315 1104 38-39 1.373096092707165 1104 40-41 1.4860693805325766 1104 42-43 1.4298017662641413 1104 44-45 1.5197971295588886 1104 46-47 1.4881039514458223 1104 48-49 1.5641615362552415 1104 50-51 1.470578081493322 1104 52-53 1.3763717294259337 1104 54-55 1.4376946089325102 1104 56-57 1.593891978017325 1104 58-59 1.48116358821256 1104 60-61 1.595877411155378 1104 62-63 1.6313877406840902 1104 64-65 1.6959123069718416 1104 66-67 1.7695537899278193 1104 68-69 1.7218456243643203 1104 70-71 1.4842828615375652 1104 72-73 1.4735251540170253 1104 74-75 1.509230170389376 1104 76-77 1.3979866655805715 1104 78-79 1.5121982218758205 1104 80-81 1.5538187784398012 1104 82-83 1.328250191209058 1104 84-85 1.3998834735750876 1104 86-87 1.4046474083233775 1104 88-89 1.427527617821866 1104 90-91 1.3266703755022107 1104 92-93 1.3027826049639906 1104 94-95 1.3530430401703306 1104 96-97 1.3562714726569034 1104 98-99 1.2922077583784848 1104 100-101 1.2944269645872595 1104 102-103 1.29006575684636 1104 104-105 1.3768542344286345 1104 106-107 1.4811388884229792 1104 108-109 1.355488389238939 1104 110-111 1.3126667932204086 1104 112-113 1.3892854884934103 1104 114-115 1.3661759499815211 1104 116-117 1.4398875381272909 1104 118-119 1.3758570862980157 1104 120-121 1.360822143883368 1105 1 0.6830296449832183 1105 2 0.8741688165176491 1105 3 0.8437967018390289 1105 4 1.0393278443392546 1105 5 1.1071639722645017 1105 6 1.1148016593256145 1105 7 1.1111415162784724 1105 8 1.0018320395812026 1105 9 1.1239885445437068 1105 10-11 1.1291508040719904 1105 12-13 0.998959396257618 1105 14-15 1.1783432903114104 1105 16-17 1.2801940134061098 1105 18-19 1.2073812646057291 1105 20-21 1.1121230781415719 1105 22-23 1.299845184152879 1105 24-25 1.289705041756683 1105 26-27 1.2863900841642035 1105 28-29 1.1485289218380856 1105 30-31 1.2551981205107836 1105 32-33 1.3040235925493775 1105 34-35 1.3671553724686092 1105 36-37 1.058134219119431 1105 38-39 1.1230545208164813 1105 40-41 1.177493859542274 1105 42-43 1.3854736393970413 1105 44-45 1.4239867433804747 1105 46-47 1.4729563837913702 1105 48-49 1.4199144117875449 1105 50-51 1.227738492680949 1105 52-53 1.4143839030502434 1105 54-55 1.4963873771948002 1105 56-57 1.653114011304453 1105 58-59 1.4759854754627213 1105 60-61 1.6031160753137215 1105 62-63 1.4894600501301767 1105 64-65 1.481992478197796 1105 66-67 1.5605428810407176 1105 68-69 1.420569175653327 1105 70-71 1.2012225676236383 1105 72-73 1.2445926514341465 1105 74-75 1.2925217864295426 1105 76-77 1.4132014488357036 1105 78-79 1.4893127969146036 1105 80-81 1.508197759163135 1105 82-83 1.3924529203342857 1105 84-85 1.3585243331522463 1105 86-87 1.3294456425556653 1105 88-89 1.4226769250187061 1105 90-91 1.3845374559578971 1105 92-93 1.2441453540120015 1105 94-95 1.366262042212309 1105 96-97 1.328089233649063 1105 98-99 1.3075763378015495 1105 100-101 1.490372006679955 1105 102-103 1.3358411010272455 1105 104-105 1.3401308555076596 1105 106-107 1.1952156196906465 1105 108-109 1.2771910707778993 1105 110-111 1.2226855924391486 1105 112-113 1.144355408580207 1105 114-115 1.1473835519403828 1105 116-117 1.2391465935293375 1105 118-119 1.3763621616376476 1105 120-121 1.415302602318878 1106 1 0.5926111081796464 1106 2 0.8786877100656199 1106 3 0.8874283978474118 1106 4 1.0620440472513195 1106 5 1.2047560951593042 1106 6 1.1103622609439867 1106 7 1.0703409501049777 1106 8 0.9904794476911576 1106 9 1.1442351500935501 1106 10-11 1.1935594013561683 1106 12-13 1.0285568503134144 1106 14-15 1.2413847937407922 1106 16-17 1.2211517989803582 1106 18-19 1.1665029086077396 1106 20-21 1.2844108795033549 1106 22-23 1.367054477478895 1106 24-25 1.222628896275289 1106 26-27 1.2052000047936602 1106 28-29 1.1866489529802493 1106 30-31 1.2881001335356572 1106 32-33 1.3546702778567479 1106 34-35 1.328859035214954 1106 36-37 1.1472812268020007 1106 38-39 1.0338929521381033 1106 40-41 1.1489822491158108 1106 42-43 1.107282206903328 1106 44-45 1.1370697981199882 1106 46-47 1.2404478456038461 1106 48-49 1.2169233733589806 1106 50-51 1.123834899591266 1106 52-53 1.3064396336219701 1106 54-55 1.390306521690384 1106 56-57 1.4533238339423349 1106 58-59 1.5669530667526246 1106 60-61 1.6163280145432068 1106 62-63 1.6093488618414185 1106 64-65 1.4569412031596087 1106 66-67 1.448963872224759 1106 68-69 1.194373025921692 1106 70-71 1.256480890839697 1106 72-73 1.2885618739491207 1106 74-75 1.4063294802760744 1106 76-77 1.43890724719477 1106 78-79 1.447808252506519 1106 80-81 1.6012156304898468 1106 82-83 1.4672443603052692 1106 84-85 1.4142785173068653 1106 86-87 1.4711390863689182 1106 88-89 1.4518887094556305 1106 90-91 1.361677479609675 1106 92-93 1.2815579306457998 1106 94-95 1.3286819485365697 1106 96-97 1.1719157321690261 1106 98-99 1.3883489363686685 1106 100-101 1.290104530369451 1106 102-103 1.0678861797714063 1106 104-105 1.1671262202573232 1106 106-107 1.3019317806250683 1106 108-109 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-0.9873643850038647 2316 112-113 -1.4314035255836792 2316 114-115 -1.3907084871212767 2316 116-117 -0.978383010982391 2316 118-119 -1.1366813860402907 2316 120-121 -1.5403014787356248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42421.0 3 7458.0 4 6525.0 5 7242.0 6 8379.0 7 9312.0 8 10643.0 9 11727.0 10 13446.0 11 15292.0 12 16479.0 13 18773.0 14 22575.0 15 23131.0 16 18580.0 17 18194.0 18 19144.0 19 20344.0 20 21875.0 21 24404.0 22 27455.0 23 30877.0 24 35567.0 25 40357.0 26 46905.0 27 54122.0 28 62609.0 29 73963.0 30 86762.0 31 104907.0 32 128204.0 33 162046.0 34 209087.0 35 279120.0 36 388028.0 37 465592.0 38 193144.0 39 1003.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.14872441843325 19.672823556527483 14.245798452929328 20.93265357210994 2 13.807143500255101 22.368761831081883 39.4412746685815 24.382820000081516 3 22.021473316992548 25.731767751328505 30.28759211150083 21.959166820178115 4 11.306205613252692 16.318279216099523 42.600346886818635 29.77516828382915 5 11.200305626257771 39.37846854120897 35.783728662577566 13.637497169955695 6 26.806499852680382 38.74050066432808 18.498044077288135 15.9549554057034 7 25.205551930693126 33.58454185752772 23.662249768706605 17.547656443072555 8 23.055674623712722 32.53914769592928 20.767627160778318 23.637550519579683 9 22.525959760981266 20.552647469594756 24.902662473095575 32.0187302963284 10-11 23.956980365392788 25.668486932332236 28.692196070115262 21.682336632159714 12-13 27.192604909046665 23.814528606103213 27.902326513422704 21.090539971427418 14-15 22.219114982361578 26.237689774160046 29.402993864703454 22.140201378774925 16-17 19.516707896260733 29.895969527767086 29.471015107555704 21.116307468416476 18-19 18.92326676941671 31.782582292536677 29.41118369420935 19.882967243837268 20-21 21.09186603420461 28.13761960338396 27.848131286583243 22.92238307582819 22-23 19.772091245005154 28.054497455249212 29.06272853299507 23.110682766750564 24-25 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28.038976974820052 28.865205554971006 21.148409933267438 50-51 19.222516884755837 28.817810581440856 31.175147891514555 20.78452464228876 52-53 20.567173695624426 30.17225202475367 27.886314812169953 21.37425946745195 54-55 19.509749613257622 28.244995507304104 29.05831241373561 23.186942465702668 56-57 21.78564887963895 27.12683609682387 28.514098954784433 22.573416068752746 58-59 23.74549316291249 27.015598747563942 27.866980276003638 21.371927813519928 60-61 21.556162704006855 27.80317789892331 30.70943523642436 19.931224160645474 62-63 20.411713695921637 28.52251648427582 29.821825509079897 21.243944310722647 64-65 19.626032326088342 31.00158785200597 28.47382452271821 20.89855529918748 66-67 20.79951110752152 28.19389869892952 27.385610793275838 23.62097940027312 68-69 19.801532425646453 29.037531755701206 28.284936458953997 22.875999359698344 70-71 22.33985188813088 28.157463378165033 27.543161630286413 21.95952310341768 72-73 21.445435318731228 26.644589194709482 30.510592018210303 21.399383468348987 74-75 19.774148620404358 30.671359120959572 28.511995251474374 21.042497007161693 76-77 20.714465440501545 28.01320265075787 28.020817854335796 23.251514054404794 78-79 21.203962929017894 28.2134710724026 28.933885580635955 21.64868041794355 80-81 22.572308331347628 27.896389659734773 28.042587161730527 21.48871484718708 82-83 21.395114197722627 27.157756904304637 30.375787430599928 21.071341467372807 84-85 20.884217442018254 28.676910596475825 29.381341434748336 21.05753052675758 86-87 20.872379918025572 29.41367803491188 28.448944831746697 21.264997215315855 88-89 20.224993976685138 28.87981170191078 27.307059325018066 23.58813499638601 90-91 20.895088610091317 26.866374317836772 28.298011196346053 23.94052587572586 92-93 22.195630725027446 28.386721828065152 28.612164763667607 20.805482683239795 94-95 21.184815741307144 27.69027438991057 29.1722901195801 21.952619749202185 96-97 18.491455911621788 28.393917474205015 31.245381755424994 21.869244858748203 98-99 20.082947439305123 29.722894220044143 28.11194733844365 22.082211002207085 100-101 19.25312169445564 27.932579778901832 29.08543500917442 23.728863517468106 102-103 20.851592113763555 26.973069480288668 29.151576880353236 23.023761525594537 104-105 22.8031947870704 27.087393427733193 28.277471251844993 21.831940533351418 106-107 20.83033467608943 27.498230452522392 30.584488017106242 21.086946854281933 108-109 19.774191773197142 27.793841320934025 30.030559745664647 22.401407160204183 110-111 19.34156149842806 29.760198071867762 28.515003903217984 22.383236526486204 112-113 20.789691948836275 27.411298445920757 27.2793458739852 24.51966373125777 114-115 19.68469414208814 27.67055733894249 28.344579388041424 24.300169130927944 116-117 21.87969853730496 27.266091044966746 27.460172901538588 23.39403751618971 118-119 21.25190625180152 26.373497636312642 29.649016108071013 22.725580003814823 120-121 20.0721323347775 29.91095759547518 27.65073450856928 22.366175561178036 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7077.0 1 8605.5 2 5784.5 3 1401.5 4 1338.5 5 1334.5 6 1381.0 7 1489.5 8 1687.0 9 1774.5 10 1692.0 11 1647.0 12 1706.0 13 1874.0 14 2057.5 15 2179.5 16 2375.0 17 2622.5 18 2893.0 19 3229.0 20 3641.0 21 4182.5 22 4964.5 23 6114.5 24 7671.5 25 9771.5 26 12855.0 27 17179.0 28 23519.5 29 31900.5 30 40923.0 31 49797.0 32 58409.0 33 65315.0 34 70444.0 35 75205.5 36 79553.0 37 83869.5 38 87576.5 39 88792.5 40 89583.5 41 90470.5 42 91277.0 43 94028.5 44 94547.0 45 95631.0 46 104151.0 47 145647.5 48 236010.5 49 229677.5 50 135137.0 51 88468.5 52 67376.5 53 55932.0 54 48353.0 55 41022.0 56 33947.5 57 27172.5 58 21260.5 59 16896.5 60 13388.5 61 10094.5 62 7507.5 63 5326.0 64 3448.0 65 2261.0 66 1537.0 67 1018.0 68 659.0 69 422.5 70 263.5 71 196.5 72 164.5 73 122.0 74 87.0 75 62.5 76 43.5 77 33.0 78 28.5 79 23.5 80 15.5 81 9.0 82 7.5 83 7.0 84 7.5 85 6.5 86 7.0 87 7.5 88 6.5 89 6.0 90 4.0 91 2.0 92 3.0 93 4.0 94 5.0 95 5.0 96 5.0 97 5.0 98 1.5 99 0.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19536323252957416 2 0.9818790971246935 3 1.0176865177723675 4 0.9843005005701304 5 0.9895101867709191 6 1.0080742798526026 7 0.9809985867808982 8 0.9718999798950138 9 0.9656997195574555 10-11 0.9536844221577493 12-13 0.9502541006100469 14-15 0.9503641644030213 16-17 0.9751285178222632 18-19 1.0110643462284072 20-21 0.9956187272809989 22-23 0.9689282574847047 24-25 0.9594627712889057 26-27 0.9638286350768906 28-29 0.9939310824553911 30-31 0.9992141445181627 32-33 0.9897670022878594 34-35 0.9968661169347087 36-37 1.010202179850108 38-39 0.9954169436605457 40-41 1.0060747876135674 42-43 0.9995076479660945 44-45 0.9779351445431106 46-47 0.9735325928241343 48-49 0.9744314471334251 50-51 0.9536477342267579 52-53 0.9735692807551257 54-55 0.9856946419478063 56-57 1.0367275539569403 58-59 1.0063682910614993 60-61 1.014476323810614 62-63 0.9788523428178972 64-65 0.9703223988623807 66-67 0.9733308092036812 68-69 0.9892533712539788 70-71 0.9703407428278764 72-73 0.9744497910989209 74-75 0.9813654660908129 76-77 0.9958205109014517 78-79 1.1065263426682106 80-81 1.0764055513242141 82-83 1.0940157582001195 84-85 1.047440429806449 86-87 1.0235749306964983 88-89 1.0214653746644888 90-91 1.0314995237906557 92-93 1.0151550505339562 94-95 1.0104773393325437 96-97 1.0211168393200698 98-99 1.0611617159972588 100-101 1.0463031039457136 102-103 1.0153384901889133 104-105 0.9968477729692129 106-107 1.0001863746894368 108-109 0.9981868824504015 110-111 1.0007550376198044 112-113 1.0042954229604812 114-115 1.0084228151970214 116-117 1.0410750737794292 118-119 1.0389104858509326 120-121 1.0124217996750917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2725692.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.508920188949794 #Duplication Level Percentage of deduplicated Percentage of total 1 81.34740419905492 45.15506567263579 2 11.968784622474029 13.287486207352808 3 2.952929776426562 4.9174182984970605 4 1.1734765802901208 2.6055367135570418 5 0.6211882919636876 1.7240745660461188 6 0.3864382326110532 1.287046140717946 7 0.2557634557726766 0.9938007277614849 8 0.19430182144527994 0.8628387439338895 9 0.13228371215038234 0.6608633418048223 >10 0.8681797764266537 8.978947308623104 >50 0.06383649806230035 2.4444717578711606 >100 0.029574625624572227 2.902410055986646 >500 0.002080352168198312 0.7795516936699808 >1k 0.002483000974942864 3.1896541942043015 >5k 6.710813445791525E-4 2.5220790089295573 >10k+ 6.039732101212372E-4 7.688755568408482 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 53544 1.9644185770072333 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 40144 1.4728003017215445 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 25173 0.9235452868482572 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 23784 0.8725857507011063 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15488 0.5682226751958769 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14861 0.5452193424642257 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11953 0.4385308391410328 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10782 0.3955692719500223 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10675 0.391643663333935 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9992 0.3665858064667615 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8210 0.3012079134399631 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7948 0.29159567552019816 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6621 0.24291079109451835 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6295 0.2309505255912994 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6150 0.22563077559753633 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6008 0.22042108939674768 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5693 0.20886439113443483 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5676 0.20824069630757985 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5112 0.18754870322839118 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4856 0.17815659289457503 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4812 0.17654232393095037 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4787 0.17562512565616364 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4624 0.16964499290455415 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4399 0.16139020843147356 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4119 0.15111758775386214 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3855 0.14143197397211424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3374 0.1237850791652175 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3194 0.11718125158675301 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3131 0.11486991193429044 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2838 0.10412034815378993 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.6687930991469324E-5 0.0 4 0.0 0.0 0.0 3.6687930991469324E-5 0.0 5 0.0 0.0 0.0 3.6687930991469324E-5 0.0 6 0.0 0.0 0.0 3.6687930991469324E-5 0.0 7 0.0 0.0 0.0 7.337586198293865E-5 0.0 8 0.0 0.0 0.0 7.337586198293865E-5 0.0 9 0.0 0.0 0.0 7.337586198293865E-5 0.0 10-11 0.0 0.0 0.0 9.171982747867332E-5 0.0 12-13 0.0 0.0 0.0 1.1006379297440797E-4 0.0 14-15 0.0 0.0 0.0 3.3019137892322393E-4 0.0 16-17 0.0 0.0 0.0 4.769431028891012E-4 0.0 18-19 0.0 0.0 0.0 7.337586198293865E-4 0.0 20-21 0.0 0.0 0.0 0.0010456060332568757 0.0 22-23 0.0 0.0 0.0 0.0014308293086673037 0.0 24-25 0.0 0.0 0.0 0.001834396549573466 0.0 26-27 0.0 0.0 0.0 0.0023113396524625674 0.0 28-29 0.0 0.0 0.0 0.003687137064642667 0.0 30-31 0.0 0.0 0.0 0.006365356027019927 0.0 32-33 0.0 0.0 0.0 0.011464978434834162 0.0 34-35 0.0 0.0 0.0 0.01590421808480195 0.0 36-37 0.0 1.8343965495734662E-5 0.0 0.0202700818727868 0.0 38-39 0.0 3.6687930991469324E-5 0.0 0.023241804283095815 0.0 40-41 0.0 3.6687930991469324E-5 0.0 0.02845149048388446 0.0 42-43 0.0 3.6687930991469324E-5 0.0 0.03566066892370818 0.0 44-45 0.0 3.6687930991469324E-5 0.0 0.04824462925378216 0.0 46-47 0.0 3.6687930991469324E-5 0.0 0.07521025853251212 0.0 48-49 0.0 3.6687930991469324E-5 0.0 0.15684090498853134 0.0 50-51 0.0 3.6687930991469324E-5 0.0 0.20064629459234573 0.0 52-53 0.0 3.6687930991469324E-5 0.0 0.23814136006562736 0.0 54-55 0.0 3.6687930991469324E-5 0.0 0.2910820444863176 0.0 56-57 0.0 3.6687930991469324E-5 0.0 0.3212395237613054 0.0 58-59 0.0 3.6687930991469324E-5 0.0 0.37199727628800316 0.0 60-61 0.0 3.6687930991469324E-5 0.0 0.49189343476812497 0.0 62-63 0.0 3.6687930991469324E-5 0.0 0.5429446907427545 0.0 64-65 0.0 3.6687930991469324E-5 0.0 0.5954451199915471 0.0 66-67 0.0 3.6687930991469324E-5 0.0 0.7948073369991914 0.0 68-69 0.0 3.6687930991469324E-5 0.0 0.9878408859108072 0.0 70-71 0.0 5.5031896487203986E-5 0.0 1.0895581745846559 0.0 72-73 0.0 7.337586198293865E-5 0.0 1.1884321486066658 0.0 74-75 0.0 7.337586198293865E-5 0.0 1.250581503706215 0.0 76-77 0.0 7.337586198293865E-5 0.0 1.2861137648714527 0.0 78-79 0.0 7.337586198293865E-5 0.0 1.354279940653603 0.0 80-81 0.0 7.337586198293865E-5 0.0 1.5017103913428222 0.0 82-83 0.0 7.337586198293865E-5 0.0 1.6902313247424874 0.0 84-85 0.0 7.337586198293865E-5 0.0 1.9517062089186892 0.0 86-87 0.0 7.337586198293865E-5 0.0 2.5771070245647714 0.0 88-89 0.0 7.337586198293865E-5 0.0 3.3268799262719337 0.0 90-91 0.0 1.1006379297440797E-4 0.0 4.109488526216461 0.0 92-93 0.0 1.1006379297440797E-4 0.0 4.899306304600813 0.0 94-95 0.0 1.1006379297440797E-4 0.0 5.597642727057936 0.0 96-97 0.0 1.1006379297440797E-4 0.0 6.307095592605474 0.0 98-99 0.0 1.1006379297440797E-4 0.0 7.036433316750388 0.0 100-101 0.0 1.1006379297440797E-4 0.0 7.7945894106891025 0.0 102-103 0.0 1.1006379297440797E-4 0.0 8.636283923495391 0.0 104-105 0.0 1.1006379297440797E-4 0.0 9.44947191392131 0.0 106-107 0.0 1.1006379297440797E-4 0.0 10.289808973280913 0.0 108-109 0.0 1.1006379297440797E-4 0.0 11.085166629244977 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3025 0.0 52.884712 2 ACCTGGG 3405 0.0 46.244915 3 GTACCTG 3995 0.0 41.079884 1 TATAACG 435 0.0 34.394962 2 GTACCCG 355 0.0 29.197475 1 GAGTACG 1405 0.0 26.640242 1 GTACGCA 1140 0.0 25.70963 3 AGTACGC 1135 0.0 25.350407 2 CCTGGGG 2595 0.0 24.79926 4 ATAACGC 650 0.0 24.755697 3 TAACGCA 585 0.0 23.572922 4 GTACCGG 155 2.6299834E-4 22.290546 1 GAGTACT 6425 0.0 21.734596 12-13 GGACCGT 215 4.631962E-6 21.382074 6 TACGCAG 1370 0.0 20.970438 4 TACCCGG 415 1.8189894E-12 20.799547 2 GTATAAC 1005 0.0 20.627073 1 GTACATG 8350 0.0 20.619867 1 ACCGTCC 225 6.9032285E-6 20.418888 8 AGCGCGT 170 4.998605E-4 20.26875 8 >>END_MODULE