##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003833_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2725692 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74513518035053 31.0 31.0 34.0 30.0 34.0 2 31.942089201567896 31.0 31.0 34.0 30.0 34.0 3 31.994507449851266 33.0 31.0 34.0 30.0 34.0 4 35.734638029535255 37.0 35.0 37.0 35.0 37.0 5 35.58786209153492 37.0 35.0 37.0 33.0 37.0 6 35.65447783535337 37.0 35.0 37.0 33.0 37.0 7 35.60485153861845 37.0 35.0 37.0 33.0 37.0 8 35.605382779859205 37.0 35.0 37.0 33.0 37.0 9 37.19897405869776 39.0 37.0 39.0 33.0 39.0 10-11 37.11140913940386 39.0 37.0 39.0 33.0 39.0 12-13 37.082470800075725 39.0 37.0 39.0 33.0 39.0 14-15 38.21056029074452 40.0 37.5 41.0 33.0 41.0 16-17 38.23439442167347 40.0 37.5 41.0 33.0 41.0 18-19 38.26931234343425 40.0 38.0 41.0 33.0 41.0 20-21 38.21445434773995 40.0 37.5 41.0 33.0 41.0 22-23 38.135439000444656 40.0 37.0 41.0 33.0 41.0 24-25 38.01480302983609 40.0 37.0 41.0 33.0 41.0 26-27 37.81998351244381 40.0 37.0 41.0 32.5 41.0 28-29 37.68368289593982 40.0 37.0 41.0 32.0 41.0 30-31 37.51915770380512 39.0 36.5 41.0 32.0 41.0 32-33 37.283917808761956 39.0 36.0 41.0 31.5 41.0 34-35 37.24820082386418 39.0 36.0 41.0 31.0 41.0 36-37 37.10438945412761 39.0 36.0 41.0 31.0 41.0 38-39 36.88889005067337 39.0 36.0 40.0 30.5 41.0 40-41 36.94601957961501 39.0 36.0 41.0 30.5 41.0 42-43 36.84008574703232 39.0 36.0 41.0 30.0 41.0 44-45 36.63441889252344 39.0 35.5 41.0 30.0 41.0 46-47 36.48986275778775 39.0 35.0 41.0 30.0 41.0 48-49 36.2727498191285 39.0 35.0 41.0 30.0 41.0 50-51 36.056755495485184 39.0 35.0 40.5 29.0 41.0 52-53 35.88713214845991 39.0 35.0 40.0 28.0 41.0 54-55 35.72328953528131 39.0 35.0 40.0 28.0 41.0 56-57 35.45720774761051 38.5 34.5 40.0 27.5 41.0 58-59 35.20123586964338 38.0 34.0 40.0 27.0 41.0 60-61 34.822725935285426 38.0 34.0 40.0 26.0 41.0 62-63 34.606709782323165 38.0 34.0 40.0 26.0 41.0 64-65 34.17895382163502 37.0 33.0 39.5 25.5 41.0 66-67 33.81037897898956 36.5 33.0 39.0 25.0 41.0 68-69 33.485963381042325 36.0 32.5 39.0 25.0 40.5 70-71 33.028490196251084 35.5 32.0 39.0 23.5 40.0 72-73 32.62732454730762 35.0 31.5 38.0 22.5 40.0 74-75 32.25859928414509 35.0 31.0 37.0 22.0 39.5 76-77 31.226001874019516 34.0 30.5 36.0 21.0 39.0 78-79 31.66664612142531 35.0 31.0 36.0 22.0 39.0 80-81 31.436425869100397 35.0 31.0 36.0 21.0 38.5 82-83 31.11011258792263 34.5 31.0 36.0 20.0 37.0 84-85 30.75738729834479 34.0 31.0 35.0 19.5 37.0 86-87 30.509730373057558 34.0 31.0 35.0 18.0 36.5 88-89 30.183584572284765 34.0 30.5 35.0 17.0 36.0 90-91 29.86893254997263 34.0 30.0 35.0 11.5 36.0 92-93 29.6180896447581 34.0 30.0 35.0 7.0 35.5 94-95 29.267493355815695 34.0 30.0 35.0 2.0 35.0 96-97 28.919205471491274 34.0 29.0 35.0 2.0 35.0 98-99 28.58810294780188 34.0 29.0 35.0 2.0 35.0 100-101 27.67961218655666 33.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.12131839693375923 1101 2 0.19304208482272145 1101 3 0.1858575806386007 1101 4 0.14093628987333773 1101 5 0.12469580582114048 1101 6 0.18690527417297886 1101 7 0.18151222042580883 1101 8 0.15756448290357383 1101 9 0.2936971189043618 1101 10-11 0.26107467685326213 1101 12-13 0.23223803316601987 1101 14-15 0.25062639394416664 1101 16-17 0.2488874269700645 1101 18-19 0.2497278653053172 1101 20-21 0.21917071289043122 1101 22-23 0.08678155273771893 1101 24-25 0.15064077435271628 1101 26-27 0.1922624476126913 1101 28-29 0.27979702769373915 1101 30-31 0.12084090727653063 1101 32-33 0.21565278524529674 1101 34-35 0.22828266841626998 1101 36-37 0.20926796197879582 1101 38-39 0.11617501277363829 1101 40-41 0.15360516569091942 1101 42-43 0.266722017133354 1101 44-45 0.2971379026764893 1101 46-47 0.24350591204797212 1101 48-49 0.2652498845096076 1101 50-51 0.21209989956354747 1101 52-53 0.196668789547509 1101 54-55 0.2720245720777754 1101 56-57 0.23099234622074505 1101 58-59 0.2529917161260755 1101 60-61 0.14279515863476178 1101 62-63 -0.006480395117264948 1101 64-65 -0.18530600672653463 1101 66-67 -0.2536954552344355 1101 68-69 -0.09654358510801586 1101 70-71 0.007168870490382062 1101 72-73 -0.04515181928571366 1101 74-75 0.031255744990161816 1101 76-77 0.011725260079540334 1101 78-79 0.05213387729651586 1101 80-81 0.060792981471017526 1101 82-83 0.1826514983270684 1101 84-85 0.24624972438240533 1101 86-87 0.033539291622940226 1101 88-89 0.1190799868212089 1101 90-91 0.1641101591008045 1101 92-93 0.1708252535642778 1101 94-95 0.23788291869374945 1101 96-97 0.2496745048818525 1101 98-99 0.16542107157796337 1101 100-101 0.3087390936334984 1102 1 0.0681943154113469 1102 2 0.10787091369925861 1102 3 0.11104485077096982 1102 4 0.05763980597102858 1102 5 0.03542309443559333 1102 6 -0.02981968961749004 1102 7 0.06200355794099721 1102 8 0.07245686049947153 1102 9 0.035429827694997584 1102 10-11 0.10304092718755697 1102 12-13 0.057779587408830935 1102 14-15 0.09684246488571802 1102 16-17 0.08237124720132982 1102 18-19 0.06215720045332063 1102 20-21 0.10024325640144127 1102 22-23 0.09464041103201737 1102 24-25 0.06478115876950596 1102 26-27 0.14249453283152036 1102 28-29 0.028112324664846255 1102 30-31 0.07435899085898257 1102 32-33 0.019279298830156222 1102 34-35 0.027059620354897618 1102 36-37 0.015228567068518828 1102 38-39 -0.02859161779382191 1102 40-41 -0.016433996207389612 1102 42-43 0.11096013418480055 1102 44-45 0.16386167492847648 1102 46-47 -0.060487330604878764 1102 48-49 -0.14853303836077458 1102 50-51 -0.030313310096921953 1102 52-53 -0.06909857447809031 1102 54-55 -0.023147313968777894 1102 56-57 -8.069033748014931E-4 1102 58-59 0.03162637189061002 1102 60-61 -0.23762071477619173 1102 62-63 -0.3716710545146569 1102 64-65 -0.5074530002101412 1102 66-67 -0.37734669986258496 1102 68-69 -0.2687460513279021 1102 70-71 -0.2334596793013617 1102 72-73 -0.21142704349354347 1102 74-75 -0.26675862231614644 1102 76-77 -0.28722777476899 1102 78-79 -0.14128591759999054 1102 80-81 -0.2747694114560133 1102 82-83 -0.2425741658232461 1102 84-85 -0.39191181116648366 1102 86-87 -0.21225126768522173 1102 88-89 -0.22925437476570565 1102 90-91 -0.2946308348541251 1102 92-93 -0.31723155899083366 1102 94-95 -0.13820899393735075 1102 96-97 -0.08732964961567546 1102 98-99 -0.04305844587542751 1102 100-101 0.13446599830777117 1103 1 -0.03190332452323119 1103 2 0.029857501751582305 1103 3 0.017935852782489548 1103 4 0.02073526631836131 1103 5 0.02098640377389671 1103 6 -0.008551047278956503 1103 7 0.0036972887519510778 1103 8 0.05905301749288583 1103 9 0.06820681578099652 1103 10-11 0.06159236972623461 1103 12-13 -0.0029597887563070913 1103 14-15 0.012436672919491798 1103 16-17 0.02434082113602898 1103 18-19 -0.08801549298273414 1103 20-21 0.0076872243450623046 1103 22-23 0.03645635172176043 1103 24-25 0.058259886074829126 1103 26-27 -0.14520124291540526 1103 28-29 -0.14053304245747 1103 30-31 0.0028202142118658458 1103 32-33 0.19231341536364255 1103 34-35 0.12171397967868103 1103 36-37 -0.04283065190016089 1103 38-39 -0.053912879056646545 1103 40-41 -0.050917656981198434 1103 42-43 0.291185793766239 1103 44-45 0.30340281984521766 1103 46-47 0.2070724658570242 1103 48-49 0.13118937814441978 1103 50-51 0.21858982247143643 1103 52-53 0.2853146240181914 1103 54-55 0.29712169563923396 1103 56-57 0.16936903761639144 1103 58-59 0.18076017471652506 1103 60-61 0.15667627129643336 1103 62-63 0.018573952475975375 1103 64-65 0.13230832155048233 1103 66-67 0.16487976364514623 1103 68-69 0.003336434866078264 1103 70-71 0.08077007214264853 1103 72-73 0.18336382973905785 1103 74-75 -0.058774399974069524 1103 76-77 0.039938948085310955 1103 78-79 0.12168656505779651 1103 80-81 0.1632551081539475 1103 82-83 0.2692024160339841 1103 84-85 0.1065933750397079 1103 86-87 0.08130311741708596 1103 88-89 -0.007831473558635338 1103 90-91 0.11013517979428045 1103 92-93 0.2610690710547665 1103 94-95 0.2452747994970963 1103 96-97 0.25593223513507724 1103 98-99 0.25732326982045706 1103 100-101 0.4813640357408211 1104 1 0.1256969399804042 1104 2 0.10950719865625302 1104 3 0.16201930827723388 1104 4 0.1447801903337833 1104 5 0.05074867346907297 1104 6 0.12449641557722657 1104 7 0.0012135985321251042 1104 8 0.05613154419888389 1104 9 0.15129199449064146 1104 10-11 0.153152188493074 1104 12-13 0.23202516869429957 1104 14-15 0.1783412676848144 1104 16-17 0.1974966855954321 1104 18-19 0.03979903205478763 1104 20-21 0.15879908934748244 1104 22-23 0.052072370942070734 1104 24-25 0.12586131106626652 1104 26-27 0.12150184409161113 1104 28-29 0.156134304809747 1104 30-31 0.09055466559125591 1104 32-33 0.31006837234348694 1104 34-35 0.31607283206572134 1104 36-37 0.37678593004724803 1104 38-39 0.4984534486235077 1104 40-41 0.505672271517426 1104 42-43 0.3296507640041 1104 44-45 0.501641642760255 1104 46-47 0.5480835846438978 1104 48-49 0.5430383554371758 1104 50-51 0.6918224460825471 1104 52-53 0.5855966581343424 1104 54-55 0.6153493461222013 1104 56-57 0.6316124028558363 1104 58-59 0.623318355115444 1104 60-61 0.5686396551693704 1104 62-63 0.38633670990446944 1104 64-65 0.45038878988394515 1104 66-67 0.45828234945281565 1104 68-69 0.39362031919390716 1104 70-71 0.39146187340583083 1104 72-73 0.5679973838205257 1104 74-75 0.567562282169412 1104 76-77 0.41066238632601326 1104 78-79 0.5306836572694955 1104 80-81 0.5477906776523866 1104 82-83 0.5610252057152785 1104 84-85 0.5194648953553624 1104 86-87 0.6023812981663461 1104 88-89 0.5014533867832256 1104 90-91 0.5527281200871847 1104 92-93 0.7094879201136237 1104 94-95 0.7991648832383333 1104 96-97 0.745362490365757 1104 98-99 0.7495618234147408 1104 100-101 0.9491909484381971 1105 1 -0.022334944932740797 1105 2 -0.0647634889441413 1105 3 0.014462360735276292 1105 4 0.02459816757908584 1105 5 -0.05775475432828614 1105 6 -0.007132747824492469 1105 7 -0.10638225342594154 1105 8 -0.007151315968343397 1105 9 0.014805859542335043 1105 10-11 -0.028290740769406852 1105 12-13 -0.038337391066121995 1105 14-15 0.03479174465782364 1105 16-17 0.10469355315016315 1105 18-19 -0.021786915086387637 1105 20-21 -0.08316259894978373 1105 22-23 -0.013061000143032686 1105 24-25 -0.09397957103505661 1105 26-27 0.030127224913925943 1105 28-29 -0.09217492872355137 1105 30-31 -0.049852115028656385 1105 32-33 0.06434842650723027 1105 34-35 -0.056603636314257244 1105 36-37 -0.10034430880408252 1105 38-39 0.06202169420247827 1105 40-41 0.22366099039996357 1105 42-43 0.37872727320252153 1105 44-45 0.3890632443870672 1105 46-47 0.31187659601913253 1105 48-49 0.24933836728087044 1105 50-51 0.35461050161254803 1105 52-53 0.5472675731970966 1105 54-55 0.3727154357260858 1105 56-57 0.24560239493536784 1105 58-59 0.3803853121640799 1105 60-61 0.43151064298623254 1105 62-63 0.40632882380272406 1105 64-65 0.11665077501376686 1105 66-67 0.3391615665212697 1105 68-69 0.3568261557023007 1105 70-71 0.31511228724772167 1105 72-73 0.2435645328004341 1105 74-75 0.3956212727483859 1105 76-77 0.4976243046235638 1105 78-79 0.48804279399118755 1105 80-81 0.3939297395261647 1105 82-83 0.4291190505903941 1105 84-85 0.3433775359595579 1105 86-87 0.5009300228015441 1105 88-89 0.4904888501835707 1105 90-91 0.3757694693798932 1105 92-93 0.4261768069228147 1105 94-95 0.43001753130828746 1105 96-97 0.40284976632696967 1105 98-99 0.5473282562742234 1105 100-101 0.5817007882205267 1106 1 -0.08478023461456985 1106 2 0.013395082923885582 1106 3 0.020995394190805428 1106 4 -0.04520947641899653 1106 5 0.06696725855228891 1106 6 0.05578216676739345 1106 7 -0.03905561946248781 1106 8 0.05262931147940009 1106 9 0.04860350498995558 1106 10-11 0.0490903919842367 1106 12-13 -0.045834926519198405 1106 14-15 0.014616501105493285 1106 16-17 0.03686174445802948 1106 18-19 -0.03438768603208331 1106 20-21 0.039662745840821856 1106 22-23 0.05447808006115906 1106 24-25 0.16109214842833808 1106 26-27 0.20151808205153543 1106 28-29 0.09732613416355207 1106 30-31 0.15645725628395724 1106 32-33 0.16864542884420075 1106 34-35 0.1353286537434002 1106 36-37 0.06354599573717223 1106 38-39 0.12787091181131416 1106 40-41 0.13807991812036846 1106 42-43 0.07097452678227256 1106 44-45 0.20481450322451167 1106 46-47 0.14017772790699468 1106 48-49 0.15351691394918987 1106 50-51 0.25788003548358773 1106 52-53 0.35714655295664954 1106 54-55 0.1638602513884706 1106 56-57 0.15469429384008038 1106 58-59 0.28730237548670345 1106 60-61 0.17682275661972113 1106 62-63 0.2762096663203053 1106 64-65 0.20597566296952863 1106 66-67 0.2146257638529363 1106 68-69 0.20766200277511615 1106 70-71 0.21313632076162747 1106 72-73 0.30139037935508384 1106 74-75 0.3927989319830445 1106 76-77 0.3627373392728295 1106 78-79 0.4086839894095853 1106 80-81 0.5007024391019925 1106 82-83 0.45336153446015715 1106 84-85 0.4021765424011612 1106 86-87 0.5450096334064085 1106 88-89 0.465904248502234 1106 90-91 0.44246591393890355 1106 92-93 0.6231236546967871 1106 94-95 0.5767827929700395 1106 96-97 0.5089746983592605 1106 98-99 0.4120401420477968 1106 100-101 0.5202278713447939 1107 1 -0.055527102431593534 1107 2 -0.1315413946941284 1107 3 -0.021766939140988484 1107 4 0.004519738326891343 1107 5 0.015814513469663893 1107 6 -0.029200466902473465 1107 7 -0.021396998501714393 1107 8 -0.07154064268829075 1107 9 -0.05575542074874562 1107 10-11 -0.08570655376158953 1107 12-13 -0.1892045032987184 1107 14-15 -0.11593482784656572 1107 16-17 -0.06999136552563812 1107 18-19 0.0014512395216286222 1107 20-21 -0.08089010931334428 1107 22-23 -0.06681105199231752 1107 24-25 -0.013209845346608518 1107 26-27 0.023189227119360112 1107 28-29 -0.06940404717700943 1107 30-31 -0.07437295141827605 1107 32-33 -0.10041336693608116 1107 34-35 -0.08335992240252921 1107 36-37 0.011496491290373001 1107 38-39 -0.04482255743108965 1107 40-41 0.0809241113838084 1107 42-43 0.0024360271776942 1107 44-45 -0.024758668917208126 1107 46-47 -0.1027677496786481 1107 48-49 -0.0026288244782719516 1107 50-51 -0.06468093166606792 1107 52-53 -0.08044103023292593 1107 54-55 6.699339846818475E-4 1107 56-57 -0.018408475060553542 1107 58-59 0.1353856043753936 1107 60-61 -0.16374693300627285 1107 62-63 0.14891146030151958 1107 64-65 2.977286147114455E-4 1107 66-67 0.03813991359649549 1107 68-69 0.05434066323214637 1107 70-71 -0.0816144689323437 1107 72-73 0.13213918961116633 1107 74-75 0.02258854733386073 1107 76-77 0.11404964184933775 1107 78-79 0.180157421930641 1107 80-81 0.21515141076550748 1107 82-83 0.31290561626459024 1107 84-85 0.2918802279480275 1107 86-87 0.1533047731463988 1107 88-89 0.06098247792278855 1107 90-91 0.04692797394177006 1107 92-93 0.17457100303623108 1107 94-95 0.0533222915432745 1107 96-97 -0.11006166687478114 1107 98-99 -0.20881749104396263 1107 100-101 0.05198998730840643 1108 1 -0.009438209795384012 1108 2 0.0028155810950707405 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2316 50-51 -1.0956804057515086 2316 52-53 -0.9727062099332286 2316 54-55 -0.8366537382460137 2316 56-57 -0.8135243425872645 2316 58-59 -0.7237786423420403 2316 60-61 -0.9248903834181377 2316 62-63 -0.7568499020508597 2316 64-65 -0.8651187981883695 2316 66-67 -0.7955587145445264 2316 68-69 -0.5364465398618705 2316 70-71 -0.6591215734524312 2316 72-73 -1.0343445203710147 2316 74-75 -0.8925853934761463 2316 76-77 -0.5553862549944348 2316 78-79 -0.6015084488538314 2316 80-81 -0.6688488965907275 2316 82-83 -0.5881409344589485 2316 84-85 -0.7739261711647636 2316 86-87 -0.927852530289254 2316 88-89 -0.7310750163871376 2316 90-91 -0.8106089754733858 2316 92-93 -0.7981340177295557 2316 94-95 -0.8514811942832878 2316 96-97 -1.023015934851479 2316 98-99 -0.894141272141443 2316 100-101 -1.2839035712142284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 22.0 5 36.0 6 88.0 7 322.0 8 709.0 9 1421.0 10 2475.0 11 4042.0 12 5941.0 13 8365.0 14 10079.0 15 13143.0 16 16269.0 17 20555.0 18 18627.0 19 14173.0 20 13138.0 21 13420.0 22 14112.0 23 15803.0 24 18456.0 25 22861.0 26 28112.0 27 36107.0 28 45758.0 29 57503.0 30 71879.0 31 88838.0 32 110454.0 33 139969.0 34 182682.0 35 246210.0 36 357268.0 37 513109.0 38 514074.0 39 119584.0 40 76.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10465730897985 20.62132935925295 14.876519645269278 20.397493686497914 2 14.639716818938615 23.39490740774712 38.38665603172286 23.57871974159141 3 22.53513837697292 26.018622036810967 30.09450492821839 21.35173465799772 4 12.175880473655864 17.012450416261267 41.28305766021986 29.528611449863007 5 11.870233320565934 39.43864530548573 35.20559182769 13.485529546258345 6 27.659287989985664 39.59955857081431 17.471086241585624 15.270067197614404 7 26.082807595282226 33.49652125038339 22.77924284915537 17.641428305179016 8 24.058551002827905 32.46533357400616 20.42692277777533 23.0491926453906 9 22.94283433344633 20.649765270617518 24.023220525283122 32.38417987065303 10-11 24.45815594718699 26.005983067786087 28.584906144934934 20.950954840091985 12-13 27.681997819269384 24.016433258049698 27.59928487884911 20.702284043831806 14-15 22.183332480327202 26.708227326684288 29.185063624168066 21.923376568820444 16-17 19.88460178185943 29.817547250386323 29.16789571235488 21.129955255399363 18-19 19.310270566153477 31.634847224117767 29.484806060259196 19.570076149469564 20-21 21.662722027013366 27.7102664773631 28.251484124423005 22.37552737120053 22-23 20.222442594394376 28.14481606872677 29.10369183311981 22.529049503759047 24-25 22.58631936403673 28.116878209276763 28.846491092904113 20.450311333782395 26-27 21.94361760323632 26.137423882810438 31.72218844739462 20.196770066558628 28-29 19.852923370008316 30.462041651408867 29.936629067044546 19.74840591153827 30-31 21.10793322900748 27.895895517596276 28.896634849115827 22.099536404280418 32-33 21.22961948421062 28.239396041276798 29.675619659221137 20.855364815291445 34-35 23.08664023165945 26.85002398596248 29.792895201981985 20.270440580396084 36-37 22.33571065818482 26.540130016527275 31.32162798070741 19.802531344580494 38-39 21.02775584196599 28.372728175315043 30.982079395057312 19.61743658766165 40-41 20.7315563068017 28.998451869543178 29.6321598740509 20.637831949604216 42-43 19.970778381506136 29.631047712804765 27.944310141028065 22.45386376466103 44-45 21.419357868737844 27.212544468104504 28.3304135875411 23.037684075616554 46-47 22.890824298250024 28.83114009903951 28.28934696077421 19.988688641936264 48-49 22.470560481759918 28.313466068747058 28.737930045166827 20.4780434043262 50-51 19.674485522651533 28.705270605647193 31.04294766633115 20.577296205370125 52-53 21.200615039634172 30.269331153569862 27.618265987398367 20.911787819397595 54-55 20.15249817292763 28.349008978336354 28.820232221631947 22.67826062710407 56-57 22.232479517463567 27.253757799453044 28.72701075040778 21.786751932675617 58-59 24.207140746127454 27.21420422201508 27.723523706388498 20.855131325468964 60-61 21.66034345139502 28.21842362527625 30.641119176999332 19.480113746329394 62-63 20.827335272158642 28.43584567938717 29.69326922600658 21.043549822447606 64-65 20.136449604494032 30.910777062618088 28.43178593646829 20.520987396419585 66-67 21.386182035710583 27.89016532269533 27.58278750267219 23.140865138921892 68-69 20.446188123998514 29.0744661546086 28.01816062398154 22.461185097411338 70-71 22.641808520748533 28.494553030756144 27.6115602193832 21.252078229112122 72-73 21.884901155376323 26.737265252273552 30.288730348109766 21.089103244240363 74-75 20.3224470298875 30.91663462582658 28.310521967297795 20.450396376988124 76-77 21.336075404385774 28.00443706279211 27.833066794047863 22.826420738774257 78-79 21.376103554285415 28.512594006306312 28.786011865289467 21.325290574118803 80-81 22.879787355035646 27.96180388675078 28.187161321803444 20.97124743641013 82-83 21.819418543603728 27.254755064162133 30.141748478265733 20.784077913968403 84-85 20.803100738790018 28.70265435362202 29.349100203876255 21.145144703711708 86-87 21.144192222297438 29.16564165303192 28.02492759780648 21.665238526864165 88-89 20.57087542204894 29.111883922278793 27.27952288062 23.037717775052272 90-91 21.46310153207893 26.634480149966492 28.244833253261437 23.657585064693137 92-93 22.24473443517418 28.500345418264278 28.37830252329601 20.876617623265524 94-95 21.415436792683348 27.933261374117723 28.947508418274314 21.703793414924615 96-97 18.6993517984136 28.397206279413883 31.147889538260475 21.75555238391204 98-99 20.531610207549996 29.716049681674487 27.77314867811993 21.97919143265559 100-101 19.607684862236198 28.068709084918446 28.5912993833706 23.732306669474752 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 181.0 1 143.5 2 242.5 3 387.0 4 317.0 5 267.0 6 399.5 7 692.0 8 1082.0 9 1355.0 10 1404.0 11 1416.5 12 1538.0 13 1735.0 14 1997.5 15 2214.0 16 2446.5 17 2735.5 18 3014.0 19 3352.5 20 3800.5 21 4329.5 22 5077.0 23 6180.5 24 7700.0 25 9696.0 26 12513.0 27 16682.5 28 22904.5 29 31016.5 30 40067.5 31 49055.5 32 57466.5 33 64902.5 34 70567.5 35 75427.0 36 79491.5 37 83427.0 38 87327.5 39 88857.5 40 89268.0 41 90234.5 42 91783.0 43 93803.5 44 93603.0 45 94267.0 46 101153.0 47 148457.0 48 251160.5 49 242975.5 50 138062.5 51 88557.5 52 67715.5 53 55973.5 54 48415.5 55 41659.5 56 34742.5 57 27630.0 58 21604.5 59 17277.0 60 13579.0 61 10012.5 62 7190.0 63 4991.0 64 3210.5 65 2008.0 66 1278.5 67 771.0 68 441.5 69 247.0 70 123.0 71 64.5 72 39.0 73 24.5 74 18.0 75 13.0 76 7.0 77 3.0 78 2.0 79 2.0 80 1.0 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050335841320295914 2 0.0031551620652663617 3 0.04321838270795086 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013757974121800997 16-17 0.0 18-19 0.0 20-21 0.009667269816252166 22-23 0.0 24-25 0.0 26-27 0.0045859913739336655 28-29 0.01137325860735549 30-31 0.009428798264807615 32-33 0.005191342235292909 34-35 0.0051179663733099704 36-37 0.018343965495734662 38-39 0.04411723701724186 40-41 0.005374781890250256 42-43 0.011464978434834162 44-45 0.010401028436081552 46-47 0.004145736202036033 48-49 6.970706888379171E-4 50-51 0.0013757974121800997 52-53 5.136310338805705E-4 54-55 7.337586198293864E-4 56-57 0.009557206023277759 58-59 0.0070624267158578445 60-61 0.005631597407190541 62-63 0.005815037062147888 64-65 0.06979878871127039 66-67 0.031900155997082576 68-69 0.02478269738473753 70-71 0.015225491361459767 72-73 0.0 74-75 1.467517239658773E-4 76-77 5.503189648720398E-4 78-79 3.8522327541042794E-4 80-81 8.071344818123251E-4 82-83 6.970706888379171E-4 84-85 6.970706888379171E-4 86-87 0.004347519822489114 88-89 3.6687930991469324E-5 90-91 6.053508613592439E-4 92-93 4.0356724090616255E-4 94-95 0.0027149068933687297 96-97 0.005539877579711868 98-99 0.006603827578464477 100-101 0.008438224128037944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2725692.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.5558497660719 #Duplication Level Percentage of deduplicated Percentage of total 1 79.49620210208133 41.779904546502756 2 13.098483185325737 13.768038289027967 3 3.2646518909748012 5.147296629617826 4 1.3160763634513681 2.766700465529134 5 0.6675738789953823 1.7542456246117586 6 0.42415541349620656 1.3375108913503644 7 0.28542184640281815 1.0500411375650953 8 0.20296201791343724 0.853347305734192 9 0.16778558913713357 0.7936302794042752 >10 0.9561723214402249 9.452660503600411 >50 0.07610762845127456 2.742654871460244 >100 0.03853175316272334 3.647109156032105 >500 0.002028622212668714 0.788333145249131 >1k 0.0022384796829442765 2.3469482500772116 >5k 7.694773910120952E-4 2.471909512992911 >10k+ 8.394298811041037E-4 9.299669391244729 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 68616 2.517379072910659 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 27480 1.0081843436455769 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 23047 0.8455467455603934 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 21065 0.7728312663353012 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 18237 0.669077797491426 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 16742 0.6142293406591794 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 15880 0.5826043441445329 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14648 0.5374048131630427 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12804 0.46975226841477324 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 12050 0.4420895684472053 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 11551 0.4237822908824621 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10835 0.3975137322925701 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7573 0.2778377013983972 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6735 0.2470932152275459 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6612 0.24258059971559517 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6354 0.23311511351979605 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6278 0.2303268307644444 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 6176 0.22658466180331452 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5920 0.2171925514694984 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5709 0.20945139803029833 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5688 0.20868095147947752 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5141 0.18861265322714377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5051 0.18531073943791154 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4826 0.17705595496483095 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4267 0.1565474015405996 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3622 0.1328836860511019 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3387 0.12426202226810659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3119 0.1144296567623928 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3041 0.11156799814505822 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2920 0.10712875849509043 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.337586198293865E-5 0.0 12-13 0.0 0.0 0.0 1.1006379297440797E-4 0.0 14-15 0.0 0.0 0.0 2.751594824360199E-4 0.0 16-17 0.0 0.0 0.0 3.668793099146932E-4 0.0 18-19 0.0 0.0 0.0 6.236948268549785E-4 0.0 20-21 0.0 0.0 0.0 9.538862057782023E-4 0.0 22-23 0.0 0.0 0.0 0.0014308293086673037 0.0 24-25 0.0 0.0 0.0 0.0017793646530862622 0.0 26-27 0.0 0.0 0.0 0.0022012758594881594 0.0 28-29 0.0 0.0 0.0 0.003650449133651198 0.0 30-31 0.0 0.0 0.0 0.006567139647473009 0.0 32-33 0.0 0.0 0.0 0.012033641365201938 0.0 34-35 0.0 0.0 0.0 0.016619632739135602 0.0 36-37 0.0 0.0 0.0 0.020893776699641778 0.0 38-39 0.0 0.0 0.0 0.023902187040942262 0.0 40-41 0.0 0.0 0.0 0.029368688758671192 0.0 42-43 0.0 0.0 0.0 0.03665124306047785 0.0 44-45 0.0 0.0 0.0 0.04967545856244947 0.0 46-47 0.0 0.0 0.0 0.07777841370191496 0.0 48-49 0.0 0.0 0.0 0.16282103774014084 0.0 50-51 0.0 0.0 0.0 0.20875432734146043 0.0 52-53 0.0 0.0 0.0 0.2482121971227857 0.0 54-55 0.0 0.0 0.0 0.30386778843684464 0.0 56-57 0.0 0.0 0.0 0.33461227460769594 0.0 58-59 0.0 0.0 0.0 0.38764467885586484 0.0 60-61 0.0 0.0 0.0 0.5132274666396643 0.0 62-63 0.0 0.0 0.0 0.5659847114053973 0.0 64-65 0.0 0.0 0.0 0.6205029768587207 0.0 66-67 0.0 0.0 0.0 0.8264690214448294 0.0 68-69 0.0 0.0 0.0 1.0265283091413115 0.0 70-71 0.0 0.0 0.0 1.1328682771200853 0.0 72-73 0.0 0.0 0.0 1.2380709192381238 0.0 74-75 0.0 0.0 0.0 1.304622826056649 0.0 76-77 0.0 0.0 0.0 1.3434019691146322 0.0 78-79 0.0 0.0 0.0 1.4155671293748524 0.0 80-81 0.0 0.0 0.0 1.5707387335032719 0.0 82-83 0.0 0.0 0.0 1.7680464263753939 0.0 84-85 0.0 0.0 0.0 2.039812275194703 0.0 86-87 0.0 0.0 0.0 2.6870790977117007 0.0 88-89 0.0 3.6687930991469324E-5 0.0 3.4633590295601997 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 435 0.0 50.24188 2 ATAACGC 495 0.0 47.029705 3 TACCTGG 2640 0.0 45.351734 2 TAACGCA 525 0.0 45.225655 4 GTACCTG 3135 0.0 40.32968 1 ACCTGGG 2955 0.0 39.872692 3 GTATAAC 940 0.0 29.32789 1 AGTACGC 880 0.0 29.154688 2 GTACCGG 210 4.1836756E-11 27.160805 1 GAGTACG 1070 0.0 25.764687 1 GTACGCA 1005 0.0 25.527544 3 CCTGGGG 2420 0.0 25.509514 4 TACGCAG 1045 0.0 24.538704 4 GTACCCG 300 0.0 23.765703 1 GTACACG 325 1.8189894E-12 21.937574 1 ACGCAGT 1290 0.0 20.246368 5 TACACGG 330 2.7284841E-11 20.156326 2 CGCAGTG 1310 0.0 19.57477 6 AGTACTT 8370 0.0 19.120295 12-13 GAGTACT 8000 0.0 18.69867 12-13 >>END_MODULE