##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003832_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1728928 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.731330627996076 31.0 31.0 34.0 30.0 34.0 2 31.918819638527456 31.0 31.0 34.0 30.0 34.0 3 31.97181837531696 33.0 31.0 34.0 30.0 34.0 4 35.71448666456903 37.0 35.0 37.0 35.0 37.0 5 35.576851089229855 37.0 35.0 37.0 33.0 37.0 6 35.63796468100465 37.0 35.0 37.0 33.0 37.0 7 35.5880383682837 37.0 35.0 37.0 33.0 37.0 8 35.592256589054024 37.0 35.0 37.0 33.0 37.0 9 37.18042451738881 39.0 37.0 39.0 33.0 39.0 10-11 37.08681043976383 39.0 37.0 39.0 33.0 39.0 12-13 37.055359448166726 39.0 37.0 39.0 33.0 39.0 14-15 38.17634337577967 40.0 37.0 41.0 33.0 41.0 16-17 38.19363761822355 40.0 37.5 41.0 33.0 41.0 18-19 38.245206278109904 40.0 38.0 41.0 33.0 41.0 20-21 38.18999576616262 40.0 37.5 41.0 33.0 41.0 22-23 38.100281214718024 40.0 37.0 41.0 33.0 41.0 24-25 37.980492536415625 40.0 37.0 41.0 33.0 41.0 26-27 37.77410424262896 40.0 37.0 41.0 32.5 41.0 28-29 37.614951576930906 39.0 37.0 41.0 32.0 41.0 30-31 37.4342398873753 39.0 36.0 41.0 32.0 41.0 32-33 37.18558638647763 39.0 36.0 41.0 31.0 41.0 34-35 37.141605087082866 39.0 36.0 41.0 31.0 41.0 36-37 36.97323804114457 39.0 36.0 41.0 30.5 41.0 38-39 36.731699353587885 39.0 35.0 40.0 30.0 41.0 40-41 36.77176464259935 39.0 35.5 41.0 30.0 41.0 42-43 36.66923000842141 39.0 35.0 41.0 30.0 41.0 44-45 36.45152429713672 39.0 35.0 41.0 30.0 41.0 46-47 36.287872022432396 39.0 35.0 41.0 30.0 41.0 48-49 36.06340489598179 39.0 35.0 41.0 29.0 41.0 50-51 35.83310727803587 39.0 35.0 40.5 28.0 41.0 52-53 35.62873988968887 39.0 35.0 40.0 28.0 41.0 54-55 35.45432198448981 39.0 35.0 40.0 27.5 41.0 56-57 35.19418101852709 38.0 34.0 40.0 26.5 41.0 58-59 34.93657659543949 38.0 34.0 40.0 26.0 41.0 60-61 34.5598061342057 38.0 34.0 40.0 26.0 41.0 62-63 34.31021621490311 37.5 33.5 40.0 25.5 41.0 64-65 33.86574860260231 37.0 33.0 39.5 23.5 41.0 66-67 33.49545498713654 36.5 32.5 39.0 23.0 41.0 68-69 33.18911198152844 36.0 32.0 39.0 23.0 40.5 70-71 32.74423371013715 35.5 32.0 38.5 22.0 40.0 72-73 32.32621774880157 35.0 31.5 38.0 21.5 40.0 74-75 31.94861237714931 35.0 31.0 37.0 20.0 39.5 76-77 30.902123165337134 34.0 30.0 36.0 20.0 39.0 78-79 31.324571931277646 34.5 31.0 36.0 20.0 39.0 80-81 31.117733358474155 35.0 31.0 36.0 19.5 38.5 82-83 30.809786179644266 34.0 31.0 35.5 18.0 37.0 84-85 30.453222748431397 34.0 31.0 35.0 17.5 37.0 86-87 30.159873343482204 34.0 30.0 35.0 12.0 36.5 88-89 29.824888890688335 34.0 30.0 35.0 7.5 36.0 90-91 29.52307007579263 34.0 30.0 35.0 4.5 36.0 92-93 29.30505405661774 34.0 30.0 35.0 2.0 35.5 94-95 28.97598598669233 34.0 29.0 35.0 2.0 35.0 96-97 28.601197967758054 34.0 29.0 35.0 2.0 35.0 98-99 28.251714357104518 34.0 29.0 35.0 2.0 35.0 100-101 27.339607548723833 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.12349633913031965 1101 2 0.30760184080034847 1101 3 0.26722924115861346 1101 4 0.1990161762507583 1101 5 0.15704872573622453 1101 6 0.20121379568215758 1101 7 0.3008604910610089 1101 8 0.24162765330916613 1101 9 0.38092805251449136 1101 10-11 0.3166289247779943 1101 12-13 0.23863701607037768 1101 14-15 0.4659319421345174 1101 16-17 0.323401560342802 1101 18-19 0.28038505381608303 1101 20-21 0.206193962957137 1101 22-23 0.21945893160155805 1101 24-25 0.26230711944594276 1101 26-27 0.17627787716856602 1101 28-29 0.47354656973843845 1101 30-31 0.35367359576868296 1101 32-33 0.29807212839826747 1101 34-35 0.550366606332787 1101 36-37 0.5578294995740478 1101 38-39 0.4450098497073114 1101 40-41 0.4286821795988729 1101 42-43 0.5211626522445414 1101 44-45 0.6328594643479732 1101 46-47 0.5830041764124019 1101 48-49 0.5145004213414026 1101 50-51 0.5518681269068182 1101 52-53 0.5360659651303621 1101 54-55 0.7123749109373492 1101 56-57 0.49992570071565723 1101 58-59 0.5208277234899512 1101 60-61 0.47141443704854424 1101 62-63 0.3124834389176314 1101 64-65 0.41138004886393276 1101 66-67 0.2927150398493694 1101 68-69 0.4869607207040687 1101 70-71 0.4498987342679399 1101 72-73 0.4404045495449651 1101 74-75 0.37490120510175373 1101 76-77 0.4258452439018683 1101 78-79 0.4733233541703541 1101 80-81 0.43781320139246915 1101 82-83 0.48815755021187357 1101 84-85 0.47123215519885875 1101 86-87 0.21632999326202196 1101 88-89 0.2645516269979886 1101 90-91 0.437245428672842 1101 92-93 0.4645327593356683 1101 94-95 0.415892548438368 1101 96-97 0.5864801262986177 1101 98-99 0.6488619811648206 1101 100-101 0.7610586484368689 1102 1 0.09408737637429354 1102 2 0.14618211552774696 1102 3 0.11090111314131335 1102 4 0.1262365889855488 1102 5 0.05908085880006553 1102 6 0.011736215446049414 1102 7 0.15392703585548162 1102 8 0.059622040322054204 1102 9 0.16383290208386114 1102 10-11 0.0828795523768946 1102 12-13 0.03303046586889735 1102 14-15 0.18297141327784772 1102 16-17 0.08766125111355905 1102 18-19 0.051330121210547475 1102 20-21 -0.013616598727786311 1102 22-23 0.10998218816398264 1102 24-25 0.14828398518483965 1102 26-27 -0.04311911293147119 1102 28-29 0.07349443075263906 1102 30-31 0.2954787633214977 1102 32-33 0.2840277523281287 1102 34-35 0.19520581227533995 1102 36-37 0.05673264979868975 1102 38-39 0.009766148225530458 1102 40-41 -0.06772924205747444 1102 42-43 0.08049359962922153 1102 44-45 0.21386864338423806 1102 46-47 0.026472320135766836 1102 48-49 -0.017245140407894155 1102 50-51 0.1485746809704409 1102 52-53 0.08959596757639332 1102 54-55 0.2565524023440773 1102 56-57 0.25412031430784765 1102 58-59 0.3946710104345996 1102 60-61 0.2537827700171178 1102 62-63 0.04149910636101595 1102 64-65 -0.010544394665039647 1102 66-67 0.0642107850506548 1102 68-69 0.09920147408022473 1102 70-71 0.06346098564221592 1102 72-73 0.08069543359043507 1102 74-75 0.11588405716865324 1102 76-77 -0.026494895392904283 1102 78-79 0.0662913498447466 1102 80-81 0.15496081265449035 1102 82-83 0.19039759552773106 1102 84-85 0.11601503816292436 1102 86-87 0.09659055945278539 1102 88-89 0.05815462402413374 1102 90-91 0.09393949512751831 1102 92-93 0.21533672962288009 1102 94-95 0.26115852163506403 1102 96-97 0.3643513108191314 1102 98-99 0.1153066816553121 1102 100-101 0.22179412998224635 1103 1 0.04862359258344284 1103 2 0.09088193940525002 1103 3 0.015082960434284587 1103 4 0.07079329291349978 1103 5 0.10472435942254066 1103 6 0.010243912227814178 1103 7 0.11411518304029755 1103 8 0.22870747770380717 1103 9 0.24497113046800933 1103 10-11 0.31039844715105147 1103 12-13 0.08769673171257608 1103 14-15 0.24069694973258748 1103 16-17 0.12575582791308904 1103 18-19 0.09655275768914606 1103 20-21 0.1953519246860438 1103 22-23 0.20166374954381894 1103 24-25 0.138974980064539 1103 26-27 0.17974642293510357 1103 28-29 0.07660140801355908 1103 30-31 0.044623964718709885 1103 32-33 0.17713397804593 1103 34-35 0.12155850819119962 1103 36-37 -0.03858033523253113 1103 38-39 0.10516082272567218 1103 40-41 0.1226251922102719 1103 42-43 0.194508735054022 1103 44-45 0.2750767652779942 1103 46-47 0.14794325338893088 1103 48-49 0.20649953752320727 1103 50-51 0.39801184052957694 1103 52-53 0.30859955211029444 1103 54-55 0.35529961264871446 1103 56-57 0.29669866267748546 1103 58-59 0.24250509585564828 1103 60-61 0.13290718961344083 1103 62-63 0.20779599203169852 1103 64-65 0.3033872541225193 1103 66-67 0.23568972387177922 1103 68-69 0.11236769254400514 1103 70-71 0.15361332016385632 1103 72-73 0.34985079715758616 1103 74-75 0.24721229525750843 1103 76-77 0.23412326515630966 1103 78-79 0.3558458808328311 1103 80-81 0.4228712287152945 1103 82-83 0.22388080180670755 1103 84-85 0.15835044205199722 1103 86-87 0.18593678229511923 1103 88-89 0.23040030020054303 1103 90-91 0.22427782023200038 1103 92-93 0.23286663613745517 1103 94-95 0.24654394888732512 1103 96-97 0.2375016161743666 1103 98-99 0.09735738140729921 1103 100-101 0.3936490037393412 1104 1 0.10853792565023923 1104 2 0.10870710067171174 1104 3 0.17318236403505338 1104 4 0.07829466574118271 1104 5 0.057786239066807354 1104 6 0.007212037453314224 1104 7 0.012161090646429784 1104 8 -0.04471846309880334 1104 9 0.12070528638953704 1104 10-11 0.06680707400949615 1104 12-13 0.13040309327327293 1104 14-15 0.2179352026939938 1104 16-17 0.12417960109306136 1104 18-19 -0.007551243447032618 1104 20-21 -0.033658418059381745 1104 22-23 0.004597949221796682 1104 24-25 0.08530744023699555 1104 26-27 0.11970172173916893 1104 28-29 0.18490624630269537 1104 30-31 0.0984723728446113 1104 32-33 0.12561494849447996 1104 34-35 0.12309511180153976 1104 36-37 -0.006153011191436519 1104 38-39 0.12825944473920714 1104 40-41 0.22669982555574109 1104 42-43 0.15033553780906317 1104 44-45 0.30057686456910204 1104 46-47 0.4320629546392567 1104 48-49 0.40204815737688904 1104 50-51 0.22287072751828418 1104 52-53 0.293816987192713 1104 54-55 0.13710609537874063 1104 56-57 0.2547909807160522 1104 58-59 0.1747607503984412 1104 60-61 0.2431010450014739 1104 62-63 0.15491656561417955 1104 64-65 0.07791238937751643 1104 66-67 0.1238633922444663 1104 68-69 0.1709357687921127 1104 70-71 0.2438527717662069 1104 72-73 0.11113043726074778 1104 74-75 0.012854062284848311 1104 76-77 -0.12404801960839507 1104 78-79 0.10653260544157916 1104 80-81 0.04690741356269612 1104 82-83 0.3056219295485221 1104 84-85 0.2623102457819222 1104 86-87 0.232580447818048 1104 88-89 0.0710390139099566 1104 90-91 0.04211609600394439 1104 92-93 0.16363074875935624 1104 94-95 0.17037275673216357 1104 96-97 0.17103795500843688 1104 98-99 0.2255538478871557 1104 100-101 0.2306043127988211 1105 1 0.06479709577864767 1105 2 0.11260633939177112 1105 3 0.04673926343032875 1105 4 -3.906488253235807E-4 1105 5 -0.06785134653623004 1105 6 0.12276991499562229 1105 7 0.09003633927902399 1105 8 0.03350265638197669 1105 9 0.05113336527721657 1105 10-11 0.045542084755766155 1105 12-13 0.10953297323788291 1105 14-15 0.18871730770037942 1105 16-17 0.2914369787609701 1105 18-19 0.09182094028441412 1105 20-21 -5.476556508057229E-4 1105 22-23 0.07969470204064777 1105 24-25 0.07083244184499904 1105 26-27 0.12887427731212142 1105 28-29 0.11478882011577696 1105 30-31 0.3416312598443554 1105 32-33 0.3734345968712489 1105 34-35 0.2479789113323676 1105 36-37 0.17274822993879724 1105 38-39 0.30866749952514994 1105 40-41 0.36758000011736414 1105 42-43 0.4284701592382518 1105 44-45 0.7368101125876265 1105 46-47 0.3850126868624102 1105 48-49 0.4028099834293357 1105 50-51 0.5010801359335062 1105 52-53 0.538359509282941 1105 54-55 0.549196496309797 1105 56-57 0.5342163982185681 1105 58-59 0.4597870001754458 1105 60-61 0.36792962655477623 1105 62-63 0.414704710617805 1105 64-65 0.23145052679625167 1105 66-67 0.3614654393144576 1105 68-69 0.357831480843096 1105 70-71 0.5063038189053728 1105 72-73 0.7177537729848424 1105 74-75 0.7246703757384942 1105 76-77 0.6982779739814653 1105 78-79 0.6753322333573593 1105 80-81 0.5661023354504948 1105 82-83 0.6579431173539625 1105 84-85 0.5827480575966284 1105 86-87 0.575780353886806 1105 88-89 0.7563576762514153 1105 90-91 0.6659539396144964 1105 92-93 0.5782924263907354 1105 94-95 0.5810209672449353 1105 96-97 0.6105580379689002 1105 98-99 0.7158799312820747 1105 100-101 0.943937451959048 1106 1 0.016574156784340488 1106 2 0.05618341336036181 1106 3 0.07437311489837484 1106 4 0.0175677326423056 1106 5 0.08766246085796325 1106 6 -0.04089622171721885 1106 7 0.04218131787117585 1106 8 0.07662431319302954 1106 9 0.04049520936403894 1106 10-11 0.10211942179495992 1106 12-13 -0.008133457046497483 1106 14-15 -0.01237252900521213 1106 16-17 0.06905748606784812 1106 18-19 -0.0312689383599718 1106 20-21 0.029194573281621672 1106 22-23 -0.04506272017242452 1106 24-25 0.07188091165097177 1106 26-27 0.03403637074412558 1106 28-29 -0.014972662534901815 1106 30-31 0.07412991589669105 1106 32-33 0.02400582228463577 1106 34-35 0.24454707902653894 1106 36-37 0.3381896166640246 1106 38-39 0.29556353325919105 1106 40-41 0.2153946077799631 1106 42-43 0.2894797594090619 1106 44-45 0.3981820067341104 1106 46-47 0.2585357741675338 1106 48-49 0.3745537221582893 1106 50-51 0.32526128101503105 1106 52-53 0.18620995554981334 1106 54-55 0.2997050041656948 1106 56-57 0.2807097432947714 1106 58-59 0.5023238967810713 1106 60-61 0.2645690901957565 1106 62-63 0.42178569442561553 1106 64-65 0.41887880192997784 1106 66-67 0.41014207978843586 1106 68-69 0.3452274440437222 1106 70-71 0.45908645777043233 1106 72-73 0.37229679265413296 1106 74-75 0.2494290370522485 1106 76-77 0.1811979242649322 1106 78-79 0.3933591466241282 1106 80-81 0.4666835689847524 1106 82-83 0.5095689127612815 1106 84-85 0.6191185220115649 1106 86-87 0.43140558502674153 1106 88-89 0.28486318738997696 1106 90-91 0.35602531181365293 1106 92-93 0.33696660772644904 1106 94-95 0.3335649362559856 1106 96-97 0.24013508524916816 1106 98-99 0.19521506798347588 1106 100-101 0.2877135445598995 1107 1 -0.1435753898925327 1107 2 -0.11205757444438547 1107 3 -0.06236192368103488 1107 4 -0.07109477944376152 1107 5 -0.07390763605513229 1107 6 -0.08415970563538622 1107 7 -0.16966096167423217 1107 8 -0.06015628713507937 1107 9 -0.1586467327315404 1107 10-11 -0.05393168286590111 1107 12-13 -0.22632248818568002 1107 14-15 -0.09762978853762405 1107 16-17 -0.0079355231326943 1107 18-19 -0.09055860466246912 1107 20-21 -0.09379448571531412 1107 22-23 -0.14570764672526337 1107 24-25 -0.17447894870877434 1107 26-27 -0.12042316440236789 1107 28-29 -0.1695828768931662 1107 30-31 -0.11309225913657883 1107 32-33 -0.22111361946677732 1107 34-35 -0.27471057038426494 1107 36-37 -0.09143505541342023 1107 38-39 -0.07516704612678637 1107 40-41 0.04094062465798487 1107 42-43 0.0772108266311875 1107 44-45 0.33782408014515397 1107 46-47 0.07247688821087195 1107 48-49 0.23645996714446227 1107 50-51 -0.06439629966261862 1107 52-53 -0.15691768926902228 1107 54-55 0.003971178505125295 1107 56-57 -0.1199208963954348 1107 58-59 -0.10825804716301235 1107 60-61 -0.5047411993068494 1107 62-63 -0.19808303566755825 1107 64-65 -0.2113103151256368 1107 66-67 -0.1662322659529849 1107 68-69 -0.2039126918539651 1107 70-71 -0.2678112134709565 1107 72-73 -0.3451746409430356 1107 74-75 -0.4543139907797986 1107 76-77 -0.34445220120887043 1107 78-79 -0.265126419902046 1107 80-81 -0.09974041628187891 1107 82-83 -0.20941484545162936 1107 84-85 -0.15171173997174137 1107 86-87 -0.16765281634818052 1107 88-89 -0.18028370206062405 1107 90-91 -0.3155752040755182 1107 92-93 -0.2852226798602864 1107 94-95 -0.24582462092612545 1107 96-97 -0.2873711933225245 1107 98-99 -0.35431022741123996 1107 100-101 -0.07186823763997197 1108 1 0.029025592427736058 1108 2 0.035888252386971686 1108 3 0.062308125705779815 1108 4 -0.06397143427429342 1108 5 0.0802293133360763 1108 6 0.06257903847052404 1108 7 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-1.395692782524705 2316 48-49 -0.8196736769588711 2316 50-51 -0.6425213154258671 2316 52-53 -0.5797850435888563 2316 54-55 -0.4532587133900918 2316 56-57 -0.5214899575850893 2316 58-59 -0.48662074749918816 2316 60-61 -0.8840226238270361 2316 62-63 -0.7850354708415139 2316 64-65 -0.7754799374365078 2316 66-67 -0.6313148620146407 2316 68-69 -0.39599767451354495 2316 70-71 -0.5953195709353807 2316 72-73 -1.0659916127245523 2316 74-75 -0.5996265974444519 2316 76-77 -0.4083134523349976 2316 78-79 -0.4273537072881872 2316 80-81 -0.6304270482733223 2316 82-83 -0.6637392553008112 2316 84-85 -0.9061356222270831 2316 86-87 -1.0339959342734275 2316 88-89 -0.8458010106964586 2316 90-91 -0.6423492883862849 2316 92-93 -0.6983442268680591 2316 94-95 -0.8410958480114097 2316 96-97 -0.9361572634192967 2316 98-99 -0.8716689693644177 2316 100-101 -1.4156324981258521 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 10.0 5 30.0 6 91.0 7 200.0 8 555.0 9 1074.0 10 1910.0 11 3066.0 12 4789.0 13 6737.0 14 7487.0 15 9463.0 16 11766.0 17 14329.0 18 13435.0 19 9942.0 20 9130.0 21 9414.0 22 9909.0 23 10744.0 24 12394.0 25 14984.0 26 18785.0 27 23638.0 28 29559.0 29 36995.0 30 45871.0 31 56488.0 32 69832.0 33 88009.0 34 114684.0 35 154544.0 36 222888.0 37 322701.0 38 319197.0 39 74236.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.428776626189055 19.930773499942998 14.225056150319077 20.415393723548874 2 14.712338513062113 23.46107683987379 38.3914787532789 23.435105893785195 3 22.203178975020684 26.619547166754426 30.178332745062868 20.99894111316202 4 11.994368764922541 17.045996131707046 41.02733023006163 29.93230487330878 5 11.692100538599641 39.39354328231135 34.430120861019084 14.484235318069924 6 26.81372503655444 39.07247728071962 18.27444520535268 15.83935247737326 7 26.18454903847934 33.35882118862092 22.83947046956264 17.617159303337097 8 24.039693960650762 31.964720335375446 20.714396435247735 23.281189268726056 9 23.389753650817156 20.471818375316957 24.31899998149142 31.819427992374465 10-11 24.212431055544243 25.68571392215292 28.825029151011496 21.276825871291344 12-13 27.89766259786411 23.712815108552814 27.769577449147683 20.619944844435395 14-15 22.520820933895305 26.409458219027144 29.457623275718962 21.612097571358586 16-17 19.72537317921857 29.434250587647377 29.603488404375426 21.23688782875863 18-19 19.035870782357623 31.364319393288792 29.788689870254863 19.811119954098725 20-21 21.42591261617843 27.863936566975315 28.33427572772852 22.37587508911773 22-23 20.004193351080364 28.090738333446602 29.06258359589977 22.84248471957326 24-25 22.298123461474393 28.021843593255475 29.025471274685817 20.654561670584314 26-27 21.953069055631584 25.906851885486088 31.99299182456656 20.147087234315766 28-29 19.876306896340285 29.78083708467682 30.49156772028224 19.851288298700652 30-31 20.98906117453039 28.180729376506324 29.111199100159336 21.71901034880395 32-33 21.04160810158915 28.14510372815161 29.881782414396657 20.93150575586258 34-35 22.799737049804076 26.503829580971566 30.140015484330718 20.556417884893637 36-37 22.53973010544365 26.395969920184008 31.450982615421374 19.613317358950965 38-39 21.371265820111272 27.85482430392668 31.300580016497502 19.473329859464553 40-41 20.495570665455816 28.78390689576386 30.20331845801893 20.51720398076139 42-43 19.811172865072734 29.485331377362726 28.318198856497624 22.385296901066916 44-45 21.114683412649608 27.44909132873613 28.366989002928733 23.06923625568552 46-47 22.543920992247415 28.72632159195485 28.144805514439426 20.584951901358306 48-49 22.36074619592487 28.188200317831686 28.890698000610215 20.560355485633227 50-51 19.993394609608124 28.621468906946706 30.933673667107993 20.45146281633718 52-53 20.946568311741192 29.988891835513563 28.21641515441679 20.848124698328455 54-55 20.079703396937955 28.573291842837396 28.976783059846955 22.370221700377694 56-57 21.928208670179675 27.476451487040833 28.70270665814012 21.892633184639372 58-59 23.758156447024056 27.03221740537567 28.119464093122833 21.090162054477446 60-61 21.737944669290005 27.940886408871606 30.557147823310533 19.764021098527856 62-63 21.168186147184894 28.071803660924715 29.739467632794636 21.020542559095755 64-65 20.316367718649964 30.475419813659254 28.867707797016916 20.340504670673866 66-67 21.111704815981543 27.997183389519932 27.976151386448738 22.91496040804979 68-69 20.33258816736613 29.239606728246997 28.073607123971783 22.354197980415094 70-71 22.42028662308922 28.679576767141228 27.43678363390086 21.463352975868695 72-73 21.716111620740275 26.98175052026518 30.0966900839018 21.205447775092743 74-75 20.441488846376863 30.3396479599288 28.52186838912675 20.696994804567584 76-77 21.483347778821784 27.817614935622863 28.07075079233287 22.628286493222475 78-79 21.418232559484647 28.443718233282482 28.92913898187444 21.208910225358427 80-81 22.53522674592293 28.055334397782644 28.290454110734153 21.118984745560272 82-83 21.524290311320517 27.237602662256176 29.958566367990926 21.27954065843238 84-85 20.85905290287951 28.33844447117943 29.544179433907264 21.2583231920338 86-87 21.383710188955142 28.89532358141927 28.274843636393083 21.446122593232506 88-89 20.546246471437623 28.698414883455136 27.646345868375548 23.10899277673169 90-91 20.980964885390215 26.54782493015831 28.71277278747882 23.75843739697266 92-93 21.65806833913512 28.477232771991094 28.624723563215564 21.239975325658218 94-95 20.899345959132205 28.101112433428245 28.987704378733476 22.011837228706074 96-97 18.915395943790106 28.34684747739191 30.55050711300169 22.18724946581629 98-99 20.303562121805466 29.57467521762545 27.870576414601572 22.251186245967517 100-101 19.25162921939769 27.916571703585614 28.905093993204307 23.92670508381239 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 213.0 1 180.5 2 396.0 3 577.0 4 398.0 5 291.0 6 394.5 7 660.0 8 952.0 9 1164.0 10 1206.0 11 1160.0 12 1271.5 13 1434.5 14 1595.0 15 1761.0 16 1973.5 17 2238.5 18 2373.5 19 2554.0 20 2845.5 21 3238.5 22 3693.0 23 4388.0 24 5306.5 25 6598.5 26 8611.0 27 11231.0 28 14922.5 29 19661.5 30 25012.5 31 30441.0 32 35449.5 33 39764.0 34 43299.5 35 46673.0 36 49667.5 37 52521.0 38 54697.0 39 55451.5 40 55931.0 41 56283.0 42 56838.5 43 57986.5 44 58066.5 45 58766.0 46 63670.0 47 91045.5 48 155885.5 49 152997.5 50 88226.0 51 58714.0 52 44427.5 53 36238.5 54 31275.5 55 27076.5 56 22739.0 57 18373.0 58 15002.5 59 12063.5 60 9524.5 61 7233.5 62 5140.0 63 3507.5 64 2295.0 65 1448.5 66 861.0 67 504.5 68 280.5 69 163.5 70 80.5 71 45.5 72 31.5 73 17.5 74 10.5 75 6.5 76 6.0 77 3.0 78 0.5 79 1.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056509004423550314 2 0.0042222695219234115 3 0.04037183734661015 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001098946861870477 16-17 0.0 18-19 0.0 20-21 0.010150798645172038 22-23 2.8919654259749393E-5 24-25 0.0 26-27 0.005205537766754891 28-29 0.012377612023172741 30-31 0.010266477262211035 32-33 0.004742823298598901 34-35 0.004829582261378149 36-37 0.018913453885876102 38-39 0.0455484554591053 40-41 0.0065358418627033624 42-43 0.011799218937977753 44-45 0.011278665161302263 46-47 0.004916341224157397 48-49 0.0014749023672472191 50-51 0.002140054415221455 52-53 0.0011278665161302264 54-55 0.0010411075533509782 56-57 0.012117335134834997 58-59 0.009109691091821059 60-61 0.005032019841196395 62-63 0.005928529123248626 64-65 0.07441027041033518 66-67 0.03525305854263451 68-69 0.02625904606785245 70-71 0.016281765348238907 72-73 1.1567861703899757E-4 74-75 3.181161968572433E-4 76-77 8.386699735327324E-4 78-79 8.965092820522312E-4 80-81 0.001243545133169224 82-83 0.0010700272076107275 84-85 0.001388143404467971 86-87 0.006073127394547372 88-89 8.675896277924818E-5 90-91 0.0010411075533509782 92-93 3.759555053767421E-4 94-95 0.003499278165429677 96-97 0.007056395639378851 98-99 0.00896509282052231 100-101 0.010758111384626774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1728928.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.134051226384095 #Duplication Level Percentage of deduplicated Percentage of total 1 82.87522842775675 48.17872774817495 2 11.408866408687857 13.264872484752651 3 2.5315427004816002 4.415064990947282 4 0.9739596600844992 2.264808830871357 5 0.5423858971031346 1.5765544763330956 6 0.3394465851394495 1.1840043101470745 7 0.23657297115738532 0.9627061656828929 8 0.15482785575043223 0.7200616397973468 9 0.12287492000206575 0.6428895204454144 >10 0.7316877994199805 7.863123281151971 >50 0.04959602152491753 1.9777568313452878 >100 0.025830476540051873 2.7145294573688514 >500 0.002493151511076104 1.0825634229223347 >1k 0.0031912339341774133 3.8967181442886414 >5k 0.0010969866648734858 4.598097263365083 >10k+ 3.9890424177217666E-4 4.657521432405697 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38478 2.2255409132132744 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17257 0.9981329471209905 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12319 0.7125224416517055 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12283 0.7104402265450036 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9427 0.5452511614133151 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8995 0.5202645801328916 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8403 0.48602370948934825 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7837 0.45328666086731195 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7802 0.4512622850691295 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7218 0.4174841288937423 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6696 0.3872920098465639 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6390 0.36959318143959724 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5954 0.34437524292509575 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5588 0.32320605600695923 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5002 0.2893122212145329 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4459 0.2579054766884451 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3877 0.22424299913009682 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3769 0.21799635380999094 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3555 0.20561874178681822 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3118 0.18034296396379723 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3060 0.1769882840696663 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2973 0.1719562642284699 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2863 0.16559394029132501 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2524 0.14598641470321494 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2521 0.14581289677765644 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2345 0.13563317847822465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2239 0.12950221177515778 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1978 0.1144061522515686 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1961 0.11342288400673713 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1873 0.10833302485702123 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1836 0.10619297044179977 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.783930851949879E-5 0.0 9 0.0 0.0 0.0 2.3135723407799515E-4 0.0 10-11 0.0 0.0 0.0 3.759555053767421E-4 0.0 12-13 0.0 0.0 0.0 5.205537766754891E-4 0.0 14-15 0.0 0.0 0.0 6.65152047974236E-4 0.0 16-17 0.0 0.0 0.0 9.5434859057173E-4 0.0 18-19 0.0 0.0 0.0 0.001243545133169224 0.0 20-21 0.0 0.0 0.0 0.0017062596013252143 0.0 22-23 0.0 0.0 0.0 0.002284652686520202 0.0 24-25 0.0 2.8919654259749393E-5 0.0 0.003152242314312684 0.0 26-27 0.0 5.783930851949879E-5 0.0 0.004077671250624665 0.0 28-29 0.0 5.783930851949879E-5 0.0 0.007229913564937349 0.0 30-31 0.0 5.783930851949879E-5 0.0 0.013534398193562718 0.0 32-33 0.0 5.783930851949879E-5 0.0 0.023193562716319012 0.0 34-35 0.0 5.783930851949879E-5 0.0 0.03215865553684133 0.0 36-37 0.0 8.675896277924818E-5 0.0 0.0404007570008699 0.0 38-39 0.0 1.1567861703899757E-4 0.0 0.052518092135704894 0.0 40-41 0.0 1.1567861703899757E-4 0.0 0.08473458698106573 0.0 42-43 0.0 1.1567861703899757E-4 0.0 0.10694488145255326 0.0 44-45 0.0 1.1567861703899757E-4 0.0 0.13300148994058747 2.8919654259749393E-5 46-47 0.0 1.1567861703899757E-4 0.0 0.19390628181161967 5.783930851949879E-5 48-49 0.0 1.1567861703899757E-4 0.0 0.21718660349071794 5.783930851949879E-5 50-51 0.0 1.1567861703899757E-4 0.0 0.2524685816876122 5.783930851949879E-5 52-53 0.0 1.1567861703899757E-4 0.0 0.2869118899109737 5.783930851949879E-5 54-55 0.0 1.1567861703899757E-4 0.0 0.32693669140646686 5.783930851949879E-5 56-57 0.0 1.1567861703899757E-4 0.0 0.5186739991486053 5.783930851949879E-5 58-59 0.0 1.1567861703899757E-4 0.0 0.6522249625201281 5.783930851949879E-5 60-61 0.0 1.1567861703899757E-4 0.0 0.7220659275574228 5.783930851949879E-5 62-63 0.0 1.1567861703899757E-4 0.0 0.8062799607618131 5.783930851949879E-5 64-65 0.0 1.1567861703899757E-4 0.0 0.8891058505617353 5.783930851949879E-5 66-67 0.0 1.1567861703899757E-4 0.0 1.1070732847174665 5.783930851949879E-5 68-69 0.0 1.1567861703899757E-4 0.0 1.3202111366118197 5.783930851949879E-5 70-71 0.0 1.1567861703899757E-4 0.0 1.3771828555035257 5.783930851949879E-5 72-73 0.0 1.1567861703899757E-4 0.0 1.4292382331710747 5.783930851949879E-5 74-75 0.0 1.1567861703899757E-4 0.0 1.6103041884913658 5.783930851949879E-5 76-77 0.0 1.1567861703899757E-4 0.0 1.814766144107794 5.783930851949879E-5 78-79 0.0 1.7351792555849637E-4 0.0 1.9486641433304337 5.783930851949879E-5 80-81 0.0 1.7351792555849637E-4 0.0 2.054654676192415 5.783930851949879E-5 82-83 0.0 1.7351792555849637E-4 0.0 2.320455218493772 5.783930851949879E-5 84-85 5.783930851949879E-5 1.7351792555849637E-4 0.0 3.0470326121157156 5.783930851949879E-5 86-87 5.783930851949879E-5 1.7351792555849637E-4 0.0 3.7417116270891557 5.783930851949879E-5 88-89 5.783930851949879E-5 2.3135723407799515E-4 0.0 4.5264753650817156 5.783930851949879E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 315 0.0 66.36675 2 ATAACGC 445 0.0 51.245052 3 TACCTGG 2565 0.0 51.124622 2 TAACGCA 460 0.0 47.48645 4 ACCTGGG 2865 0.0 44.938168 3 GTACCCG 265 0.0 44.851723 1 GTACCTG 3005 0.0 44.615894 1 GTATAAC 595 0.0 42.34907 1 TACCCGG 285 0.0 40.010574 2 CCTGGGG 1635 0.0 36.304626 4 GTACCGG 140 4.5110937E-10 33.95916 1 GTACACG 245 0.0 29.107851 1 TCACGCA 245 0.0 27.135117 4 TATCACG 265 1.8189894E-12 25.100975 2 TACCGGG 165 2.4254841E-6 23.03639 2 CTGGGGG 1080 0.0 22.863846 5 TACGCAG 635 0.0 22.434544 4 GAGTACG 660 0.0 22.330719 1 AGTACGC 575 0.0 22.310244 2 ACAACGC 225 7.143535E-8 21.114859 3 >>END_MODULE