##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003831_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4447334 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771782375688446 31.0 31.0 34.0 30.0 34.0 2 31.94604430429556 31.0 31.0 34.0 30.0 34.0 3 32.01194963094744 33.0 31.0 34.0 30.0 34.0 4 35.72451720513908 37.0 35.0 37.0 35.0 37.0 5 35.59369680802027 37.0 35.0 37.0 33.0 37.0 6 35.67976342680806 37.0 35.0 37.0 33.0 37.0 7 35.63231387613343 37.0 35.0 37.0 33.0 37.0 8 35.642604085953515 37.0 35.0 37.0 33.0 37.0 9 37.23373845994027 39.0 37.0 39.0 33.0 39.0 10-11 37.135705683449906 39.0 37.0 39.0 33.0 39.0 12-13 37.11264973577428 39.0 37.0 39.0 33.0 39.0 14-15 38.19474003976315 40.0 37.0 41.0 33.0 41.0 16-17 38.252119472025264 40.0 38.0 41.0 33.0 41.0 18-19 38.29375036819812 40.0 38.0 41.0 33.5 41.0 20-21 38.22519390268417 40.0 37.5 41.0 33.5 41.0 22-23 38.13129697477185 40.0 37.0 41.0 33.0 41.0 24-25 37.98277417437053 40.0 37.0 41.0 33.0 41.0 26-27 37.77435706425467 39.5 37.0 41.0 32.5 41.0 28-29 37.67563230016005 39.0 37.0 41.0 32.0 41.0 30-31 37.52776674744915 39.0 36.5 41.0 32.0 41.0 32-33 37.28423129452386 39.0 36.0 41.0 31.0 41.0 34-35 37.21340627890777 39.0 36.0 41.0 31.0 41.0 36-37 37.063594504033205 39.0 35.5 41.0 30.5 41.0 38-39 36.83800688232546 39.0 35.0 40.0 30.0 41.0 40-41 36.97836850121893 39.0 36.0 41.0 31.0 41.0 42-43 36.86153693426219 39.0 36.0 41.0 30.0 41.0 44-45 36.62595557248455 39.0 35.0 41.0 30.0 41.0 46-47 36.4857178480411 39.0 35.0 41.0 30.0 41.0 48-49 36.21891249903875 39.0 35.0 41.0 29.0 41.0 50-51 35.97435677194472 39.0 35.0 40.0 28.0 41.0 52-53 35.879562452471525 39.0 35.0 40.0 28.0 41.0 54-55 35.715310116127995 39.0 35.0 40.0 28.0 41.0 56-57 35.36912159059787 38.0 34.5 40.0 27.5 41.0 58-59 35.080066956967926 38.0 34.0 40.0 26.5 41.0 60-61 34.60375766695283 38.0 34.0 40.0 26.0 41.0 62-63 34.44059609644789 37.0 34.0 40.0 26.0 41.0 64-65 34.02223995319443 37.0 33.0 39.5 25.5 41.0 66-67 33.59750684342575 36.0 32.5 39.0 24.5 40.5 68-69 33.20687989703494 36.0 32.0 39.0 24.0 40.0 70-71 32.68344034875726 35.0 31.5 38.5 23.0 40.0 72-73 32.2330880702911 35.0 31.0 37.0 22.0 39.5 74-75 31.89530604177694 35.0 31.0 37.0 22.0 39.0 76-77 30.9145624097493 34.0 30.5 36.0 20.5 38.5 78-79 31.34368432863374 34.0 31.0 36.0 21.0 38.5 80-81 31.068531506740893 34.0 31.0 36.0 20.0 37.0 82-83 30.729540551710308 34.0 30.5 35.0 19.0 37.0 84-85 30.380520442134546 34.0 30.0 35.0 18.0 36.5 86-87 30.239732612841763 34.0 31.0 35.0 18.0 36.0 88-89 29.930436751545983 34.0 30.0 35.0 13.0 36.0 90-91 29.569050806618076 34.0 30.0 35.0 7.5 35.5 92-93 29.33239284479196 34.0 29.0 35.0 4.5 35.0 94-95 28.946460621127173 34.0 29.0 35.0 2.0 35.0 96-97 28.573528208135478 34.0 29.0 35.0 2.0 35.0 98-99 28.304571457866665 34.0 29.0 35.0 2.0 35.0 100-101 27.340822276896677 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09009909373783742 1101 2 0.16321029224006978 1101 3 0.1736305721884186 1101 4 0.1422675343653097 1101 5 0.1344810538646044 1101 6 0.20668162145183544 1101 7 0.18818712969693507 1101 8 0.13008422197977154 1101 9 0.1958041389169054 1101 10-11 0.22469491978880285 1101 12-13 0.20873620807730475 1101 14-15 0.3134573002461636 1101 16-17 0.3068911759251307 1101 18-19 0.2274551611317719 1101 20-21 0.14487585381053947 1101 22-23 0.090670251698441 1101 24-25 0.28938665741911507 1101 26-27 0.2732155584280065 1101 28-29 0.30076752387754624 1101 30-31 0.2846087916971385 1101 32-33 0.3178989745642866 1101 34-35 0.3246837199027013 1101 36-37 0.26614975338898716 1101 38-39 0.2597656006094766 1101 40-41 0.32647304423198165 1101 42-43 0.3177288582199651 1101 44-45 0.4203270955453817 1101 46-47 0.39442755423686293 1101 48-49 0.3356319030885828 1101 50-51 0.2368029247875043 1101 52-53 0.23549110648332316 1101 54-55 0.2523855375471129 1101 56-57 0.18344939600118693 1101 58-59 0.23025835342188117 1101 60-61 0.2065209917221864 1101 62-63 0.20736275206037647 1101 64-65 0.09230375649882916 1101 66-67 8.759362690540229E-4 1101 68-69 0.19724677219314657 1101 70-71 0.23184976837399063 1101 72-73 0.22661846284769638 1101 74-75 0.16135876397613558 1101 76-77 0.19139086728877075 1101 78-79 0.2091781885042323 1101 80-81 0.09721462299667039 1101 82-83 0.2593770250557377 1101 84-85 0.3096739858897486 1101 86-87 0.1151099199957244 1101 88-89 0.24471670829348469 1101 90-91 0.2705990841561814 1101 92-93 0.350801002539729 1101 94-95 0.34003736288029174 1101 96-97 0.27462535406219146 1101 98-99 0.32393522209908454 1101 100-101 0.3823669084013268 1102 1 0.07436851674769684 1102 2 0.1071160569548617 1102 3 0.1176605852709649 1102 4 0.11760301775415627 1102 5 0.142874493892009 1102 6 0.021807685133879318 1102 7 0.06291760500217691 1102 8 0.07187051342233985 1102 9 0.16989219087310659 1102 10-11 0.16114921600987486 1102 12-13 0.0941557538256177 1102 14-15 0.0662047332143203 1102 16-17 0.13236175862054012 1102 18-19 0.10183502827260327 1102 20-21 0.05488715004731404 1102 22-23 0.20650873666852476 1102 24-25 0.21520362136055837 1102 26-27 0.07699081074645875 1102 28-29 0.010349717521073387 1102 30-31 0.06462910393802446 1102 32-33 0.08740601095451694 1102 34-35 0.16137416906332902 1102 36-37 0.08286223370886603 1102 38-39 0.04778971716220326 1102 40-41 0.10738580780230933 1102 42-43 0.16391104241157706 1102 44-45 0.3448602876458935 1102 46-47 0.2844097619388464 1102 48-49 0.27999360770282067 1102 50-51 0.36085992958034296 1102 52-53 0.30960267733757263 1102 54-55 0.25482194439180716 1102 56-57 0.32516732738231013 1102 58-59 0.28068843277022637 1102 60-61 0.22512109606863362 1102 62-63 0.09030357912712361 1102 64-65 0.03964885081669678 1102 66-67 0.004441433627633273 1102 68-69 0.0643180372692953 1102 70-71 0.0931613773088742 1102 72-73 0.20728050319271318 1102 74-75 0.10660302440958347 1102 76-77 0.04680773783707437 1102 78-79 0.17863392605755735 1102 80-81 0.24568489654269499 1102 82-83 0.1327036174992564 1102 84-85 0.16729335389421962 1102 86-87 0.05297857040508802 1102 88-89 0.050264536536094084 1102 90-91 0.13362959013750597 1102 92-93 0.09422159721212253 1102 94-95 0.07206385333180876 1102 96-97 0.13807580075917159 1102 98-99 0.14267056650719923 1102 100-101 0.35423326902374797 1103 1 -0.06592383284697689 1103 2 0.006521257317658069 1103 3 0.03152955112351563 1103 4 0.027539201288149684 1103 5 -0.01913138083616417 1103 6 9.220500211597482E-4 1103 7 0.04264357652021289 1103 8 0.07318839298918789 1103 9 0.02185616458810813 1103 10-11 0.05985775904126456 1103 12-13 -0.023844170603858572 1103 14-15 0.03481790976183419 1103 16-17 0.07045873023324845 1103 18-19 -0.04752124762265453 1103 20-21 -0.08179921235724663 1103 22-23 -0.01903366808045348 1103 24-25 -0.04824756001127639 1103 26-27 -0.0784023720065079 1103 28-29 -0.06617769758363323 1103 30-31 -0.03848268180030345 1103 32-33 -0.020240954421588242 1103 34-35 0.014948891995096858 1103 36-37 -0.04678638636382715 1103 38-39 0.03760171313015093 1103 40-41 -0.05972832306011355 1103 42-43 0.2523726418170966 1103 44-45 0.36786067863534555 1103 46-47 0.24206467034614576 1103 48-49 0.27363647412271064 1103 50-51 0.2047049471599749 1103 52-53 0.24151972690167867 1103 54-55 0.2754098343367417 1103 56-57 0.13977196825234017 1103 58-59 0.15702688092809325 1103 60-61 0.1544428126680515 1103 62-63 0.09734545210606882 1103 64-65 0.11470074461004032 1103 66-67 0.17988010496233642 1103 68-69 0.06852845324441859 1103 70-71 0.20384619661203374 1103 72-73 0.27953154739999064 1103 74-75 0.09052681681491137 1103 76-77 0.044323796912639324 1103 78-79 0.19752418776587533 1103 80-81 0.3048064419843506 1103 82-83 0.320820463618837 1103 84-85 0.11647894603466824 1103 86-87 0.09631149609095502 1103 88-89 0.152152499573166 1103 90-91 0.291604833612066 1103 92-93 0.24538059584034144 1103 94-95 0.26004161176100027 1103 96-97 0.4815571182486984 1103 98-99 0.41142218067605185 1103 100-101 0.5187849089515844 1104 1 0.017122891421120556 1104 2 0.036831255656395 1104 3 0.08903295177913506 1104 4 0.050359039734395594 1104 5 -0.02399279692344436 1104 6 -0.02885634401408055 1104 7 -0.07752926067103516 1104 8 -0.13650022702722708 1104 9 0.066513886749199 1104 10-11 -0.010047384460648345 1104 12-13 0.0018787668388853263 1104 14-15 0.0425682794340716 1104 16-17 0.011475324774757212 1104 18-19 -0.09505295554951942 1104 20-21 -0.20294627364799567 1104 22-23 -0.09497758645813548 1104 24-25 0.00171222425474582 1104 26-27 -0.033715701096213024 1104 28-29 0.10040162939085207 1104 30-31 0.0336521733161419 1104 32-33 0.09180633072852373 1104 34-35 -7.837663060357158E-4 1104 36-37 0.018288263578355668 1104 38-39 0.1529529387642654 1104 40-41 0.16001061185619392 1104 42-43 0.05174596718951818 1104 44-45 0.31263403131170975 1104 46-47 0.31841321328652583 1104 48-49 0.3125261580056531 1104 50-51 0.36270310825647556 1104 52-53 0.2947933205434765 1104 54-55 0.2928595153248139 1104 56-57 0.3105297840034211 1104 58-59 0.2969574039300156 1104 60-61 0.36078851007796686 1104 62-63 0.24390737693472175 1104 64-65 0.2476449146444608 1104 66-67 0.3185381988516838 1104 68-69 0.24254020709452817 1104 70-71 0.2276833533185183 1104 72-73 0.2889328181842856 1104 74-75 0.21327395442508745 1104 76-77 0.16341779975158843 1104 78-79 0.2329512064888064 1104 80-81 0.37406138999996585 1104 82-83 0.33169812061234794 1104 84-85 0.3988785999655313 1104 86-87 0.42314863141484693 1104 88-89 0.26766327229094244 1104 90-91 0.2591486802758034 1104 92-93 0.35534554482639535 1104 94-95 0.4718194761657166 1104 96-97 0.3557358208512902 1104 98-99 0.2918723995187271 1104 100-101 0.4544887500887427 1105 1 -0.08687332670287518 1105 2 -0.09069747102248371 1105 3 -0.08877108902644792 1105 4 -0.09021930795108801 1105 5 -0.10450664500018547 1105 6 -0.041326148993192646 1105 7 -0.024257454449205795 1105 8 -0.011693305048112279 1105 9 -0.12127058213179964 1105 10-11 -0.13709316987140596 1105 12-13 -0.0504468541175811 1105 14-15 0.016254516234660343 1105 16-17 -0.014273305817248172 1105 18-19 -0.16696106599032134 1105 20-21 -0.19005047042015377 1105 22-23 -0.03598049986355534 1105 24-25 0.021085743601943818 1105 26-27 -0.10590727317121207 1105 28-29 -0.1629002062157312 1105 30-31 -0.11141052667830564 1105 32-33 -0.031184323811793035 1105 34-35 -0.040095937607837584 1105 36-37 0.02513575119145628 1105 38-39 -0.004099848130955763 1105 40-41 0.10002311066988057 1105 42-43 0.16402059012107628 1105 44-45 0.3313967561963409 1105 46-47 0.09012166502731134 1105 48-49 0.09285700052955548 1105 50-51 0.16270883837163552 1105 52-53 0.2162443647674337 1105 54-55 0.15610906363057353 1105 56-57 0.18598143347079343 1105 58-59 0.2496963094422071 1105 60-61 0.20836995381628753 1105 62-63 0.08225241223760094 1105 64-65 -0.11255567519926757 1105 66-67 0.007857980462674163 1105 68-69 -0.07800655333115714 1105 70-71 0.11502499173750635 1105 72-73 0.11177001307803636 1105 74-75 0.12958275749356574 1105 76-77 0.10969689575429697 1105 78-79 0.19510146801484396 1105 80-81 0.13663944043571874 1105 82-83 0.14348640001992052 1105 84-85 0.1751034568469514 1105 86-87 0.14975409328898692 1105 88-89 0.20667138935719365 1105 90-91 0.1378451865171506 1105 92-93 0.19304719563675832 1105 94-95 0.11419600561402987 1105 96-97 0.2398524788332459 1105 98-99 0.280313503783546 1105 100-101 0.43195972795080095 1106 1 0.023109582717051325 1106 2 0.03655195091377905 1106 3 0.010270498051735188 1106 4 0.04072469526596478 1106 5 0.08856664342848575 1106 6 0.04422728420274069 1106 7 0.08927761475089113 1106 8 0.10210985168255604 1106 9 -0.04229367117036986 1106 10-11 0.08211446392101607 1106 12-13 -0.0016297903462643148 1106 14-15 0.021976509465453375 1106 16-17 -0.058122372537532385 1106 18-19 -0.07028200027536968 1106 20-21 -0.0042951338027492625 1106 22-23 0.0252230455940321 1106 24-25 0.0606998651126176 1106 26-27 0.09815341514351417 1106 28-29 -0.04171626465880962 1106 30-31 0.021280234355167238 1106 32-33 0.10271701997695715 1106 34-35 0.19713220488068828 1106 36-37 0.2696022570056087 1106 38-39 0.2836963048129846 1106 40-41 0.3133956518045551 1106 42-43 0.3921170010992654 1106 44-45 0.44019840493465523 1106 46-47 0.5535277171033144 1106 48-49 0.5206660551858988 1106 50-51 0.5863290253626801 1106 52-53 0.5947588247386122 1106 54-55 0.49449417136825247 1106 56-57 0.35674062384644145 1106 58-59 0.5641001497960829 1106 60-61 0.389990878048053 1106 62-63 0.45375889660475366 1106 64-65 0.454751050446653 1106 66-67 0.3073871517370961 1106 68-69 0.31335935263714276 1106 70-71 0.4363991078864302 1106 72-73 0.5404754854147527 1106 74-75 0.5411515722115716 1106 76-77 0.5090635389633071 1106 78-79 0.46870573730876686 1106 80-81 0.5570551436628257 1106 82-83 0.6287667190440835 1106 84-85 0.572145278501722 1106 86-87 0.6224175429052892 1106 88-89 0.5896302379212521 1106 90-91 0.5033741567259291 1106 92-93 0.5212156551387856 1106 94-95 0.5952916458405646 1106 96-97 0.6313901283758234 1106 98-99 0.6356635255656542 1106 100-101 0.7486548789175949 1107 1 -0.06617117393033212 1107 2 -0.04476287246089328 1107 3 0.0507362444365036 1107 4 0.036823845148170165 1107 5 -0.06795110659933101 1107 6 -0.018844451682170416 1107 7 -0.03843787906474461 1107 8 0.024124097471329264 1107 9 0.06511383372056656 1107 10-11 0.061125295489432574 1107 12-13 -0.07354240755384467 1107 14-15 0.13268634231912557 1107 16-17 0.01847968979193837 1107 18-19 0.07187001617941746 1107 20-21 0.023490145532832685 1107 22-23 0.07039641510790773 1107 24-25 0.16314176940339564 1107 26-27 0.06940713517504093 1107 28-29 0.08875585634746841 1107 30-31 0.11961469021800752 1107 32-33 0.101661133151012 1107 34-35 0.029549637066807577 1107 36-37 0.09075569452986798 1107 38-39 0.2554714482119138 1107 40-41 0.22495156422995422 1107 42-43 0.1861018594502255 1107 44-45 0.2651784481868944 1107 46-47 0.11808373985869736 1107 48-49 0.07966321328733272 1107 50-51 0.1360903135746554 1107 52-53 0.16199575802647104 1107 54-55 0.13632931961786454 1107 56-57 0.10460935015044015 1107 58-59 0.13628868211159073 1107 60-61 -0.11627719930408631 1107 62-63 0.0644927364464678 1107 64-65 0.08540987911717224 1107 66-67 0.0467572540411183 1107 68-69 0.06835905577977286 1107 70-71 0.12276439461099642 1107 72-73 0.12133787171056554 1107 74-75 -0.030313505462903123 1107 76-77 -0.061721440870822875 1107 78-79 -0.044512918939283 1107 80-81 0.1416642125474361 1107 82-83 0.10897144006185044 1107 84-85 0.13363312405512318 1107 86-87 0.09615630168571698 1107 88-89 0.09409316404078183 1107 90-91 0.08892094036641751 1107 92-93 0.06792306818421068 1107 94-95 0.13813294228281947 1107 96-97 0.062439384585854185 1107 98-99 0.011554788752672351 1107 100-101 0.1843482403934864 1108 1 0.013972027413739596 1108 2 -0.019808437010681246 1108 3 0.01641235538447816 1108 4 -0.03292580601540607 1108 5 0.004625749328582174 1108 6 0.03375750408165601 1108 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27.0 5 57.0 6 178.0 7 427.0 8 1227.0 9 2187.0 10 3847.0 11 6193.0 12 9497.0 13 13682.0 14 16593.0 15 21320.0 16 26241.0 17 33661.0 18 31118.0 19 24261.0 20 22581.0 21 23492.0 22 24718.0 23 27419.0 24 31683.0 25 38445.0 26 48223.0 27 60689.0 28 76392.0 29 96128.0 30 119878.0 31 148927.0 32 185060.0 33 235179.0 34 308818.0 35 419494.0 36 618329.0 37 860246.0 38 767118.0 39 143940.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.08417681806759 19.458585951302524 14.024872703940853 20.432364526689035 2 14.5834012608089 24.11808025148719 37.2340906356775 24.064427852026412 3 25.84459801430896 24.735682829292564 28.926652519364065 20.49306663703441 4 12.195665987758058 16.23390552632206 41.79701816863766 29.773410317282217 5 11.734243481600437 39.910224867302524 35.13052988599462 13.225001765102418 6 27.87557669381252 39.5796223085561 16.79774894352437 15.747052054107021 7 26.461313676912958 33.76888266093799 22.07349391792926 17.696309744219796 8 23.82751104369494 30.267436626077554 19.776679691698444 26.12837263852906 9 22.09710356811519 21.91677081145693 24.393243232912123 31.592882387515758 10-11 25.325981812924326 24.809380181475014 27.871877848616723 21.992760156983937 12-13 30.003405411354915 23.130365644942074 26.0320931632404 20.834135780462606 14-15 22.789500962951386 26.083817807124156 29.87939778717931 21.24728344274515 16-17 20.11186926819528 29.040465591295817 29.238055877970936 21.60960926253796 18-19 19.404782730507762 32.687931691210956 28.368456248170254 19.538829330111028 20-21 22.387016536288048 26.815279512617508 26.648858581337176 24.148845369757264 22-23 20.30613171846324 27.738674450805807 28.042822958653428 23.912370872077517 24-25 24.35412991243743 27.172717407777334 27.643426376341424 20.829726303443817 26-27 23.240720971234076 24.511092604604208 32.0603420060941 20.187844418067613 28-29 19.791927974808722 31.537815867782903 29.026615341109373 19.643640816298994 30-31 21.4981557242261 27.052188984717894 27.632946113190915 23.816709177865093 32-33 22.059628420844806 27.668260073927257 28.91216786588215 21.35994363934578 34-35 24.84597465323567 25.768265960794697 28.974911944959796 20.410847441009842 36-37 23.402636520524283 25.064891968913926 31.25036194779698 20.282109562764813 38-39 21.167119684723726 28.129002056296315 31.151471946885874 19.552406312094085 40-41 21.21136006760435 29.323994710202335 28.377120028531266 21.087525193662046 42-43 19.78108396136654 29.9275054820086 26.254722757998472 24.03668779862639 44-45 22.338984038025124 25.62231980191819 27.10982235850348 24.928873801553213 46-47 24.753149173656023 28.583820221586155 27.236903262539187 19.426127342218635 48-49 23.750826913278328 27.57161597405325 28.241668694131356 20.43588841853707 50-51 18.85432055459241 28.307092279913565 32.05674093761735 20.78184622787668 52-53 21.848261440259932 31.19195689507368 26.00479505766155 20.954986607004834 54-55 19.993436444194206 27.53764684944262 28.00700067648022 24.461916029882953 56-57 23.340674158010856 25.765024047516192 27.98631876569801 22.90798302877494 58-59 27.122018122099977 25.844129881496197 26.03672273061108 20.99712926579275 60-61 22.582538763795064 27.468573633558517 31.13568014118085 18.81320746146557 62-63 20.658039806693935 28.00499253323727 29.754414227348025 21.58255343272077 64-65 19.86089919258573 32.17345489737493 27.42533111230468 20.54031479773466 66-67 22.1599703092227 27.132208307794393 25.854593916764607 24.853227466218307 68-69 20.401705584961956 28.53778038024014 27.02560705264802 24.034906982149877 70-71 24.34467402592788 27.604976969137613 26.596721750930254 21.453627254004257 72-73 23.280655629701208 25.05812760584868 30.78340868757698 20.877808076873134 74-75 20.153137170250847 31.763444051698553 27.800844103864797 20.282574674185803 76-77 21.602016053551562 27.31966175001588 26.618438930115502 24.459883266317053 78-79 22.025673747845744 27.970520577010056 28.02962378006188 21.974181895082314 80-81 24.502419280400538 26.764851476521617 27.546562870204088 21.18616637287375 82-83 22.911815873776813 25.861899936073527 30.106871657098683 21.119412533050973 84-85 20.966997009867125 28.455573209236896 29.68460146776894 20.892828313127037 86-87 21.449092112331662 29.559277197784343 27.024434679737112 21.967196010146882 88-89 20.144878052016193 29.657233396288756 25.525301549144185 24.67258700255086 90-91 22.29834842247313 25.212469111831727 26.84348357040074 25.645698895294405 92-93 24.11105312353774 28.260187668374137 27.165481718961537 20.46327748912658 94-95 22.835701803569183 27.211917945399243 28.442656007098382 21.50972424393319 96-97 18.373397762931145 27.954152265183158 31.94425845866152 21.72819151322418 98-99 21.198461816440474 30.727983676841582 26.21582940244103 21.857725104276913 100-101 19.29953373610642 27.47899743593088 27.871489809163275 25.349979018799424 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 380.0 1 298.5 2 477.5 3 717.5 4 570.5 5 480.5 6 705.0 7 1334.5 8 2093.0 9 2514.5 10 2514.5 11 2486.5 12 2714.5 13 3041.5 14 3390.5 15 3733.0 16 4105.5 17 4497.0 18 4898.5 19 5494.0 20 6040.5 21 6690.0 22 7852.0 23 9438.5 24 11486.5 25 14086.5 26 17783.5 27 22632.0 28 29177.5 29 37490.5 30 45882.0 31 54020.5 32 62587.0 33 70699.5 34 78376.0 35 87643.0 36 97406.5 37 107771.0 38 117468.0 39 123094.5 40 127484.0 41 132514.0 42 137681.5 43 144586.0 44 148044.0 45 151775.0 46 167068.5 47 285989.5 48 539228.0 49 520000.5 50 269930.5 51 155742.5 52 114502.5 53 95089.0 54 82685.0 55 71310.5 56 59255.0 57 46683.5 58 36325.5 59 29208.5 60 23214.5 61 17492.5 62 12750.0 63 8976.5 64 6035.5 65 3980.5 66 2508.0 67 1483.0 68 859.0 69 472.0 70 269.0 71 132.5 72 64.5 73 40.5 74 24.0 75 16.0 76 11.5 77 5.5 78 3.0 79 3.0 80 3.0 81 1.5 82 0.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05353769246924112 2 0.004249737033467691 3 0.0388547385917046 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.124269056472934E-5 14-15 0.0015514912979326491 16-17 0.0 18-19 0.0 20-21 0.01073676948931652 22-23 0.0 24-25 0.0 26-27 0.005610102591799941 28-29 0.012513114598543755 30-31 0.010489430296892475 32-33 0.005846199093659257 34-35 0.00578998564083561 36-37 0.020360512612724837 38-39 0.04413880315712739 40-41 0.006363362859636806 42-43 0.01157997128167122 44-45 0.010894167157222731 46-47 0.004834356942833617 48-49 0.0016189474413210252 50-51 0.002338479637463703 52-53 0.0010455702225198287 54-55 0.0011130263659082048 56-57 0.011535000519412305 58-59 0.008724327878229968 60-61 0.0061160236672127615 62-63 0.006588216670931395 64-65 0.07076149441440648 66-67 0.03393044012435315 68-69 0.025127413412170077 70-71 0.01555988374158541 72-73 1.4615497734148144E-4 74-75 3.1479533581242153E-4 76-77 7.082895055779485E-4 78-79 6.408333621895724E-4 80-81 9.106579357430766E-4 82-83 8.319591017899714E-4 84-85 8.994152451783472E-4 86-87 0.005688801425753047 88-89 1.6864035847094012E-4 90-91 9.106579357430766E-4 92-93 4.721930037186324E-4 94-95 0.002799429950617606 96-97 0.0069592254595674625 98-99 0.009005395142348202 100-101 0.011152749040211506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4447334.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.46100931305337 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59433445390334 30.181620610404007 2 14.982826357281553 12.124405535556608 3 4.325627609681882 5.250577770004181 4 1.8707407326049155 3.0276823281694307 5 1.0444284414247238 2.112931444765178 6 0.6211723447639571 1.50799560159034 7 0.4170983757860326 1.1813354886996668 8 0.3033883191870837 0.9820318086480161 9 0.23394214660088236 0.8518981835100596 >10 1.3951975090509852 10.619354433368475 >50 0.12789574632637962 3.5860725860085236 >100 0.07377695338876072 5.298817639626228 >500 0.005200433184636727 1.447631270219823 >1k 0.002600216585971642 2.1168174056202678 >5k 6.08561328631661E-4 1.643496084618633 >10k+ 0.001161798900114989 18.067331809190502 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 183089 4.116825945611461 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 68479 1.5397764143642012 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 67410 1.5157395419368098 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 64190 1.443336614699953 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 51515 1.1583344088840641 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 41564 0.9345823812648207 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 38808 0.8726126708720326 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 38786 0.8721179924871845 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 35866 0.8064606795891651 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 30558 0.6871082765539984 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 30275 0.6807449136943616 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 27774 0.6245089754895855 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 18287 0.41119016471441094 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 16737 0.37633782396374993 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 16053 0.36095782327120024 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 14817 0.3331658921951893 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 14735 0.3313220909425737 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 14382 0.32338475140387474 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 12253 0.27551337497925726 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 10796 0.24275217467363597 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10760 0.24194270095297543 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 9210 0.20709036020231447 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 8815 0.19820863465617827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8274 0.18604404346514114 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 7688 0.17286761012327836 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 7540 0.16953977371611847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5527 0.12427670150251813 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5459 0.12274769558571494 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 5334 0.11993702294453262 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 5247 0.11798079478626972 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5177 0.1164068181072076 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5150 0.11579971281671221 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 4866 0.10941386457594596 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 4853 0.10912155462126298 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 4820 0.10837953704399086 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 4558 0.10248836718807267 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.2485381129458684E-5 0.0 4 0.0 0.0 0.0 2.2485381129458684E-5 0.0 5 0.0 0.0 0.0 2.2485381129458684E-5 0.0 6 0.0 0.0 0.0 2.2485381129458684E-5 0.0 7 0.0 0.0 0.0 2.2485381129458684E-5 0.0 8 0.0 0.0 0.0 4.497076225891737E-5 0.0 9 0.0 0.0 0.0 6.745614338837604E-5 0.0 10-11 0.0 0.0 0.0 6.745614338837604E-5 0.0 12-13 0.0 0.0 0.0 8.994152451783474E-5 0.0 14-15 0.0 0.0 0.0 2.5858188298877484E-4 0.0 16-17 0.0 0.0 0.0 5.621345282364671E-4 0.0 18-19 0.0 0.0 0.0 0.0010005994602609114 0.0 20-21 0.0 0.0 0.0 0.0016301901318857546 2.2485381129458684E-5 22-23 0.0 0.0 0.0 0.002574576139323019 2.2485381129458684E-5 24-25 0.0 0.0 0.0 0.0033165937165951557 2.2485381129458684E-5 26-27 0.0 0.0 0.0 0.004272222414597149 3.372807169418803E-5 28-29 0.0 0.0 0.0 0.006509517836978288 4.497076225891737E-5 30-31 0.0 0.0 0.0 0.010354518010115724 4.497076225891737E-5 32-33 0.0 0.0 0.0 0.01859541019406233 4.497076225891737E-5 34-35 0.0 0.0 0.0 0.027938086053352414 4.497076225891737E-5 36-37 0.0 0.0 0.0 0.03780916836918477 4.497076225891737E-5 38-39 0.0 1.1242690564729342E-5 0.0 0.05302052870326357 4.497076225891737E-5 40-41 0.0 2.2485381129458684E-5 0.0 0.06885023701840248 4.497076225891737E-5 42-43 0.0 2.2485381129458684E-5 0.0 0.08298229905826726 4.497076225891737E-5 44-45 0.0 2.2485381129458684E-5 0.0 0.09269598370619342 4.497076225891737E-5 46-47 0.0 2.2485381129458684E-5 0.0 0.10808722708930789 4.497076225891737E-5 48-49 0.0 2.2485381129458684E-5 0.0 0.11822813397869375 4.497076225891737E-5 50-51 0.0 2.2485381129458684E-5 0.0 0.12887496194349243 4.497076225891737E-5 52-53 0.0 2.2485381129458684E-5 0.0 0.15260828172563606 4.497076225891737E-5 54-55 0.0 2.2485381129458684E-5 0.0 0.188832230725194 4.497076225891737E-5 56-57 0.0 2.2485381129458684E-5 0.0 0.24855340300503626 4.497076225891737E-5 58-59 0.0 2.2485381129458684E-5 0.0 0.3268250147166819 4.497076225891737E-5 60-61 0.0 2.2485381129458684E-5 0.0 0.4184866708909203 4.497076225891737E-5 62-63 0.0 2.2485381129458684E-5 0.0 0.4626816875008713 4.497076225891737E-5 64-65 0.0 2.2485381129458684E-5 0.0 0.5212336199619818 4.497076225891737E-5 66-67 0.0 2.2485381129458684E-5 0.0 0.6996213911525422 4.497076225891737E-5 68-69 0.0 2.2485381129458684E-5 0.0 0.9024282862496948 4.497076225891737E-5 70-71 0.0 2.2485381129458684E-5 0.0 1.0356541694417374 4.497076225891737E-5 72-73 0.0 2.2485381129458684E-5 0.0 1.1824387374548437 4.497076225891737E-5 74-75 0.0 2.2485381129458684E-5 0.0 1.3075586407497166 4.497076225891737E-5 76-77 0.0 2.2485381129458684E-5 0.0 1.3890569046534396 4.497076225891737E-5 78-79 0.0 2.2485381129458684E-5 0.0 1.4718818060438008 4.497076225891737E-5 80-81 0.0 2.2485381129458684E-5 0.0 1.5559546460868465 4.497076225891737E-5 82-83 0.0 2.2485381129458684E-5 0.0 1.6875166110753095 4.497076225891737E-5 84-85 0.0 2.2485381129458684E-5 0.0 2.011025032075396 4.497076225891737E-5 86-87 0.0 2.2485381129458684E-5 0.0 2.573755422911794 4.497076225891737E-5 88-89 0.0 2.2485381129458684E-5 0.0 3.2576932607265388 4.497076225891737E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 665 0.0 44.294502 2 TACCTGG 3855 0.0 43.873817 2 ACCTGGG 4175 0.0 39.369095 3 ATAACGC 835 0.0 38.117477 3 GTACCTG 4780 0.0 36.693024 1 TAACGCA 805 0.0 35.981503 4 GTACACG 415 0.0 26.342972 1 GTACCCG 465 0.0 25.554775 1 AGTACGC 2350 0.0 25.473139 2 TCACGCA 505 0.0 25.387371 4 GTATAAC 1280 0.0 25.251312 1 CCTGGGG 3555 0.0 24.576702 4 TACGCAG 2455 0.0 23.983665 4 GTACGCA 2445 0.0 23.703735 3 GAGTACG 2675 0.0 23.277296 1 CGCAGTG 3005 0.0 19.910007 6 ACGCAGT 2960 0.0 19.73144 5 CTGGGGG 2245 0.0 19.458834 5 TACACGG 550 0.0 19.00377 2 ACACGGG 630 0.0 18.096996 3 >>END_MODULE