##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003830_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2887742 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.916619282470524 31.0 31.0 34.0 28.0 34.0 2 30.64573704991651 31.0 31.0 34.0 27.0 34.0 3 30.804396653163614 31.0 31.0 34.0 27.0 34.0 4 34.21706475162947 37.0 35.0 37.0 30.0 37.0 5 34.14897175717221 37.0 35.0 37.0 30.0 37.0 6 34.27372632319646 37.0 35.0 37.0 30.0 37.0 7 34.23658727130055 37.0 35.0 37.0 30.0 37.0 8 34.30483505797956 37.0 35.0 37.0 30.0 37.0 9 35.767268682590064 39.0 35.0 39.0 30.0 39.0 10-11 35.680938255564385 39.0 35.0 39.0 30.0 39.0 12-13 35.692872493456825 39.0 35.0 39.0 30.0 39.0 14-15 36.645613077622585 39.0 36.0 41.0 30.0 41.0 16-17 36.62693446990763 39.0 36.0 41.0 30.0 41.0 18-19 36.55239941795354 39.5 36.0 41.0 30.0 41.0 20-21 36.47919914590708 39.5 36.0 41.0 29.5 41.0 22-23 36.291930858089124 39.0 36.0 41.0 28.5 41.0 24-25 36.201484066097315 39.0 36.0 41.0 28.0 41.0 26-27 36.05624758028937 39.0 36.0 41.0 28.0 41.0 28-29 35.89812091938961 39.0 35.5 40.0 27.0 41.0 30-31 35.63314381963485 39.0 35.0 40.0 27.0 41.0 32-33 35.384065473993175 38.0 35.0 40.0 26.0 41.0 34-35 35.303025685812656 38.0 35.0 40.0 25.5 41.0 36-37 35.26942677012005 38.0 35.0 40.0 25.5 41.0 38-39 35.20665004006591 38.5 35.0 40.0 25.0 41.0 40-41 35.33268657657089 39.0 35.0 40.5 25.0 41.0 42-43 35.2239322280176 39.0 35.0 41.0 25.0 41.0 44-45 35.02350556247754 39.0 35.0 40.5 24.0 41.0 46-47 34.7619190010742 38.5 34.0 40.0 23.5 41.0 48-49 34.47638137340524 38.0 34.0 40.0 23.0 41.0 50-51 33.66071207192332 37.5 33.5 39.5 20.5 40.5 52-53 33.78123599684459 38.0 33.0 40.0 20.5 40.5 54-55 34.14180266104105 38.0 34.0 40.0 21.5 41.0 56-57 33.86488318554774 38.0 33.5 40.0 18.5 41.0 58-59 33.65770176144545 38.0 33.0 40.0 18.0 41.0 60-61 33.188604626036536 38.0 32.0 40.0 13.0 41.0 62-63 33.049023423837724 37.0 32.0 40.0 13.0 41.0 64-65 32.71674252755267 37.0 32.0 39.5 12.0 41.0 66-67 32.28048437152626 36.0 31.0 39.0 8.0 41.0 68-69 31.95228694253157 36.0 31.0 39.0 8.0 41.0 70-71 31.507828781103022 35.0 31.0 38.5 7.0 40.0 72-73 31.09333174501046 35.0 30.5 37.5 4.0 40.0 74-75 30.668513842303085 35.0 30.0 37.0 2.0 39.5 76-77 30.230909651901037 35.0 29.5 36.5 2.0 39.0 78-79 29.66837168971466 34.0 29.0 36.0 2.0 39.0 80-81 29.2380896562089 34.0 29.0 36.0 2.0 37.5 82-83 28.914612351103386 34.0 28.5 35.0 2.0 37.0 84-85 28.67066136794769 34.0 28.5 35.0 2.0 37.0 86-87 28.500154272784755 34.0 29.0 35.0 2.0 36.0 88-89 28.102367351376955 34.0 27.5 35.0 2.0 36.0 90-91 27.80665066339029 34.0 26.0 35.0 2.0 36.0 92-93 27.617168708284883 34.0 26.0 35.0 2.0 35.0 94-95 27.324343033415033 34.0 25.5 35.0 2.0 35.0 96-97 27.068345440832317 33.5 25.0 35.0 2.0 35.0 98-99 26.81619670316808 33.0 25.0 35.0 2.0 35.0 100-101 26.479443108144704 33.0 25.0 35.0 2.0 35.0 102-103 26.192446728274202 33.0 24.0 35.0 2.0 35.0 104-105 25.879087189922092 33.0 22.5 35.0 2.0 35.0 106-107 25.46962886573662 32.0 19.5 35.0 2.0 35.0 108-109 25.224181038333757 32.0 19.0 35.0 2.0 35.0 110-111 24.902199192310118 32.0 18.0 34.5 2.0 35.0 112-113 24.343759241649703 32.0 10.0 34.0 2.0 35.0 114-115 23.861226695459635 31.0 4.5 34.0 2.0 35.0 116-117 23.26032588783901 31.0 2.0 34.0 2.0 35.0 118-119 22.56213401335715 31.0 2.0 34.0 2.0 35.0 120-121 20.988493951329446 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5979084796389458 1101 2 0.5641074903311214 1101 3 0.6765479345209968 1101 4 0.9330158866085796 1101 5 0.9652241542408078 1101 6 0.8353623881657768 1101 7 0.8935350253952734 1101 8 0.7462726867856802 1101 9 0.8906881309410366 1101 10-11 0.8287273196895981 1101 12-13 0.6239051063388388 1101 14-15 0.9375460153303905 1101 16-17 0.9172347504602669 1101 18-19 1.0491432356307229 1101 20-21 0.9532915771459685 1101 22-23 1.121131464392569 1101 24-25 0.9902250817632208 1101 26-27 0.8479336927345003 1101 28-29 0.9214798449299195 1101 30-31 1.0637217278952207 1101 32-33 1.0706183767400859 1101 34-35 1.0126654602644436 1101 36-37 1.0345154321535688 1101 38-39 0.9044555509182288 1101 40-41 1.0655266417918767 1101 42-43 1.0448690466783646 1101 44-45 1.0199252072537988 1101 46-47 1.1011666756624976 1101 48-49 1.3197349539341943 1101 50-51 1.056568388876265 1101 52-53 1.088645842159508 1101 54-55 1.1445621465067362 1101 56-57 1.158033802041281 1101 58-59 1.111640936481642 1101 60-61 1.3445673304244607 1101 62-63 1.1673671555226761 1101 64-65 1.2051299465771308 1101 66-67 1.1257290866924734 1101 68-69 1.1136234458754508 1101 70-71 0.8308419658530788 1101 72-73 0.7756144828529692 1101 74-75 0.8529783012776662 1101 76-77 0.7945120901851297 1101 78-79 1.0167089177367536 1101 80-81 1.0620796500394967 1101 82-83 0.9615570960490061 1101 84-85 0.865020434317767 1101 86-87 1.0430147280927038 1101 88-89 1.0375850175665207 1101 90-91 0.9586404995102633 1101 92-93 0.9991223524711117 1101 94-95 1.1507657525550634 1101 96-97 1.116250414015738 1101 98-99 1.1284681998800714 1101 100-101 1.196506061622447 1101 102-103 1.0541321077286803 1101 104-105 1.0880220910148246 1101 106-107 0.9465097286350961 1101 108-109 0.8173571867597254 1101 110-111 1.054975564230336 1101 112-113 0.8969502342442084 1101 114-115 0.9215359446134563 1101 116-117 0.9051356937922961 1101 118-119 0.9929425372522402 1101 120-121 1.0947372774439117 1102 1 0.4589142283761518 1102 2 0.5588532123419263 1102 3 0.554923077166773 1102 4 0.8820149613573847 1102 5 0.9865561422575269 1102 6 0.9374645837861664 1102 7 0.8961623373986995 1102 8 0.8321459626662389 1102 9 0.9168903173573497 1102 10-11 0.780057861352546 1102 12-13 0.7624407112088321 1102 14-15 0.9264897134147674 1102 16-17 0.8773534483543273 1102 18-19 0.9961438854518647 1102 20-21 0.9552846388000873 1102 22-23 1.0520396984361398 1102 24-25 0.8641566657697268 1102 26-27 0.9848625646546836 1102 28-29 0.8843900402015095 1102 30-31 0.8996235502317589 1102 32-33 0.8232671418376967 1102 34-35 0.8662576078783388 1102 36-37 0.8514380134729009 1102 38-39 0.9464695327993624 1102 40-41 0.8800163730261232 1102 42-43 1.0609538500676692 1102 44-45 1.0983501072374011 1102 46-47 1.23714451807637 1102 48-49 1.037930222765688 1102 50-51 0.7463478233865359 1102 52-53 0.8184737052998372 1102 54-55 0.9375849428414398 1102 56-57 1.140569080270616 1102 58-59 1.0480963524536762 1102 60-61 1.2257703023008375 1102 62-63 1.0599182952010011 1102 64-65 1.021456858514945 1102 66-67 1.0557923456389346 1102 68-69 1.099354716113158 1102 70-71 0.6621663207204769 1102 72-73 0.6919327097478138 1102 74-75 0.9611233491076838 1102 76-77 0.8147442611067746 1102 78-79 0.9573267530088216 1102 80-81 0.888193844167855 1102 82-83 0.8724456140069776 1102 84-85 0.833796666780728 1102 86-87 0.7443085918849341 1102 88-89 0.8394378382677843 1102 90-91 0.8681387185574287 1102 92-93 0.7697503675823931 1102 94-95 0.660510820592517 1102 96-97 0.7114980000236137 1102 98-99 0.8161301793916742 1102 100-101 0.8211108229636928 1102 102-103 0.7061515013258344 1102 104-105 0.7160673058329614 1102 106-107 0.6209550450440169 1102 108-109 0.43753655538824177 1102 110-111 0.5151809395452993 1102 112-113 0.5478014722607512 1102 114-115 0.6263278738210154 1102 116-117 0.6857252109800314 1102 118-119 0.4888361728112294 1102 120-121 0.5641282102590495 1103 1 0.6590631210927249 1103 2 0.9005844062624249 1103 3 0.8608129258066661 1103 4 0.9070913066167634 1103 5 1.065077943293268 1103 6 1.1294845286166009 1103 7 1.0344773492851118 1103 8 0.9496693049963554 1103 9 1.0377281266718228 1103 10-11 1.0368249079409608 1103 12-13 0.931389125732565 1103 14-15 1.0841954540404473 1103 16-17 1.1020124363839443 1103 18-19 1.119486710931774 1103 20-21 1.18590086876668 1103 22-23 1.3232257732872768 1103 24-25 1.268061655610019 1103 26-27 1.2878213827975173 1103 28-29 1.1690854399544008 1103 30-31 1.1627094555735482 1103 32-33 1.1461671341477384 1103 34-35 1.148945244074369 1103 36-37 1.0379315664088438 1103 38-39 0.9810414450092679 1103 40-41 1.059951168772777 1103 42-43 0.9836484072274274 1103 44-45 1.1195216749559904 1103 46-47 1.053634009015191 1103 48-49 1.0355431507673742 1103 50-51 0.8758452113950099 1103 52-53 0.8673419838386707 1103 54-55 0.8439557913836779 1103 56-57 0.8532874008211664 1103 58-59 0.8118554831013753 1103 60-61 1.0215933803888362 1103 62-63 1.0423824743780088 1103 64-65 1.0641824868860823 1103 66-67 0.9126976463800389 1103 68-69 0.9288677199293609 1103 70-71 0.685567387030094 1103 72-73 0.5661776901620037 1103 74-75 0.8032684091106006 1103 76-77 0.8564464879398273 1103 78-79 0.8733907249815118 1103 80-81 0.7898874988188389 1103 82-83 0.8909012813893291 1103 84-85 0.7379112434059856 1103 86-87 0.7993545500090917 1103 88-89 0.8731425070168761 1103 90-91 1.0067278326882239 1103 92-93 0.8123539237405666 1103 94-95 0.8875525166862523 1103 96-97 0.9003643537136377 1103 98-99 0.8838084295908821 1103 100-101 0.9456297876781008 1103 102-103 0.9077117139461421 1103 104-105 1.1084207260015084 1103 106-107 1.209188250856112 1103 108-109 1.1649773745435077 1103 110-111 1.091201262740622 1103 112-113 1.2744110209261628 1103 114-115 1.260937551753237 1103 116-117 1.2217807428681233 1103 118-119 1.153682371258423 1103 120-121 1.0284679092749727 1104 1 0.7146605697003743 1104 2 0.8961753315620271 1104 3 0.9299047377163276 1104 4 1.095641440551475 1104 5 1.1867915530314974 1104 6 1.1555270104432225 1104 7 1.2405139650026342 1104 8 1.0693929406696014 1104 9 1.1498983558030176 1104 10-11 1.1541166641104184 1104 12-13 0.9908544943907529 1104 14-15 1.237408730919583 1104 16-17 1.2955940976124936 1104 18-19 1.278562445807978 1104 20-21 1.2121710578574252 1104 22-23 1.3078684335312474 1104 24-25 1.2717177507042692 1104 26-27 1.248104599031791 1104 28-29 1.1945046781147752 1104 30-31 1.3235144147481108 1104 32-33 1.3245953698484882 1104 34-35 1.2662489880393935 1104 36-37 1.0099333700548385 1104 38-39 0.9948769432451812 1104 40-41 1.1219967126596728 1104 42-43 1.1163281115852683 1104 44-45 1.144210266104004 1104 46-47 1.228547447394881 1104 48-49 1.1365257240420163 1104 50-51 0.9378364755171873 1104 52-53 1.144230913565849 1104 54-55 1.1594980596206454 1104 56-57 1.1176011329931157 1104 58-59 1.0757747742027775 1104 60-61 1.321378999658883 1104 62-63 1.2529949594541137 1104 64-65 1.1896460859752551 1104 66-67 1.1370496056239716 1104 68-69 1.0738849348906179 1104 70-71 0.9509614812456597 1104 72-73 0.802973772282666 1104 74-75 1.0334351185763353 1104 76-77 0.95684135497266 1104 78-79 1.0023139897024649 1104 80-81 1.1934336956450586 1104 82-83 1.1473683624158753 1104 84-85 1.0652188332018753 1104 86-87 1.0942077694928507 1104 88-89 1.173517292970832 1104 90-91 1.0269890936095898 1104 92-93 0.8654070388027151 1104 94-95 0.9610530381857849 1104 96-97 0.9294732771365766 1104 98-99 1.0642542052012587 1104 100-101 1.0984867775365466 1104 102-103 1.0764060421975437 1104 104-105 1.052824676984205 1104 106-107 1.1548460522335553 1104 108-109 1.1788561992283881 1104 110-111 1.161534450435422 1104 112-113 1.1298303564493501 1104 114-115 1.1723359372344966 1104 116-117 0.8989160613479505 1104 118-119 0.6692492236040728 1104 120-121 0.4968784857966604 1105 1 0.7422752857481463 1105 2 0.9892311291367655 1105 3 0.8794491078765532 1105 4 1.156478084498282 1105 5 1.11366587842479 1105 6 1.2069721261339623 1105 7 1.1544191684220166 1105 8 1.0436458744644384 1105 9 1.13829555921739 1105 10-11 1.1562330957433815 1105 12-13 1.1274534360809056 1105 14-15 1.2596896506286868 1105 16-17 1.2484870869679625 1105 18-19 1.309078425133201 1105 20-21 1.1832650458502059 1105 22-23 1.297355162035032 1105 24-25 1.1923529121146998 1105 26-27 1.216404696689139 1105 28-29 1.205507301950945 1105 30-31 1.2417879982464015 1105 32-33 1.373208923786315 1105 34-35 1.4042560634963053 1105 36-37 1.1758975891031582 1105 38-39 1.131823076252239 1105 40-41 1.1168240070746478 1105 42-43 1.164233304975653 1105 44-45 1.0768419875686916 1105 46-47 1.184617184020901 1105 48-49 1.1272169350057126 1105 50-51 0.9716910450413181 1105 52-53 1.155949607740375 1105 54-55 1.2352888773223185 1105 56-57 1.4402706763102273 1105 58-59 1.2515418357193866 1105 60-61 1.2986869537812424 1105 62-63 1.18634914474233 1105 64-65 1.2875132318607925 1105 66-67 1.1915262988633017 1105 68-69 1.1380586646868842 1105 70-71 0.981742602161507 1105 72-73 0.9563528545542859 1105 74-75 1.0563663560165821 1105 76-77 1.1392994494382833 1105 78-79 1.2722014894360711 1105 80-81 1.243763022812228 1105 82-83 0.998164288637561 1105 84-85 1.228930915760575 1105 86-87 1.1678958200690808 1105 88-89 1.093239627671032 1105 90-91 0.9551352211007007 1105 92-93 0.9887131726622371 1105 94-95 1.0865642976146113 1105 96-97 1.0857912314219043 1105 98-99 1.1222075753827951 1105 100-101 1.1317532091068259 1105 102-103 0.9060742579262673 1105 104-105 0.8935029756151174 1105 106-107 0.8977779917035456 1105 108-109 0.9086582393769653 1105 110-111 0.9967567243348334 1105 112-113 0.9624173861991139 1105 114-115 0.7377210444256619 1105 116-117 0.7983126291746778 1105 118-119 0.7271354621913879 1105 120-121 0.7825173391313633 1106 1 0.6022642658935737 1106 2 0.8327690780414798 1106 3 0.7497099424971054 1106 4 0.9984991028040113 1106 5 1.1811457433818617 1106 6 1.0160196569749047 1106 7 0.9625905004744126 1106 8 0.9075284319079131 1106 9 1.1022293438101087 1106 10-11 1.0461013964462182 1106 12-13 0.872923182237038 1106 14-15 1.0945119201536073 1106 16-17 1.0734232754169 1106 18-19 1.068474342449754 1106 20-21 1.07385531447094 1106 22-23 1.1704953653529202 1106 24-25 1.0408212779110784 1106 26-27 1.0430800704014018 1106 28-29 0.9980123618746717 1106 30-31 1.121248396886763 1106 32-33 1.1567203224810996 1106 34-35 1.1802128966433614 1106 36-37 1.0585940889126917 1106 38-39 1.0446784482481988 1106 40-41 1.0673228307862814 1106 42-43 1.1407673200120527 1106 44-45 1.0273290379314162 1106 46-47 1.0882792711164697 1106 48-49 0.9886219873945024 1106 50-51 0.9002362358637654 1106 52-53 1.0014641711113441 1106 54-55 1.0873925916307599 1106 56-57 1.1736126957587558 1106 58-59 1.2333374911966644 1106 60-61 1.212932496652975 1106 62-63 1.1211535711029015 1106 64-65 1.0874264753608571 1106 66-67 1.1779297814722298 1106 68-69 0.967776140941357 1106 70-71 0.7922356386180702 1106 72-73 0.8599817146429451 1106 74-75 0.9828845686024508 1106 76-77 0.8532934089254063 1106 78-79 0.890557657833682 1106 80-81 0.9867738396419448 1106 82-83 0.9029121401322016 1106 84-85 0.8207880228865356 1106 86-87 0.8795246445760618 1106 88-89 0.9665578701873692 1106 90-91 0.769553884527344 1106 92-93 0.8833916712829222 1106 94-95 0.9415482963252977 1106 96-97 0.7906444936337458 1106 98-99 0.9900174603911864 1106 100-101 1.008856844926271 1106 102-103 0.810312585043274 1106 104-105 0.8448704108460028 1106 106-107 0.8095589802043861 1106 108-109 0.7543670630048744 1106 110-111 0.6705503223539857 1106 112-113 0.5796495922212088 1106 114-115 0.6582411281891147 1106 116-117 0.6271556072354336 1106 118-119 0.6507338842486838 1106 120-121 0.5225616644818416 1107 1 0.6844034612846457 1107 2 0.9661875810088532 1107 3 0.8646856935630822 1107 4 1.0664338205507562 1107 5 1.1956111547457695 1107 6 1.0546968423626168 1107 7 1.1152375401515258 1107 8 0.9698098117644847 1107 9 1.05673302726116 1107 10-11 1.01930191668621 1107 12-13 0.9414411356704093 1107 14-15 1.0686929083383632 1107 16-17 1.1479210051307902 1107 18-19 1.2186236605502003 1107 20-21 1.1205585172831576 1107 22-23 1.2873030094632014 1107 24-25 1.319859376404061 1107 26-27 1.0592720655407675 1107 28-29 1.1065890660810709 1107 30-31 1.2029847317715863 1107 32-33 1.1910006933216195 1107 34-35 1.1594494078347566 1107 36-37 0.9408019760547859 1107 38-39 1.1119693079732684 1107 40-41 1.2105218727635148 1107 42-43 1.1412591475245577 1107 44-45 1.1316927610921326 1107 46-47 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-0.5410712243288138 2316 112-113 -0.9353131018703422 2316 114-115 -0.7338906019984215 2316 116-117 -0.4747122088565199 2316 118-119 -0.7073839318432711 2316 120-121 -1.0103721427373529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44687.0 3 7769.0 4 6923.0 5 7749.0 6 9007.0 7 9760.0 8 11407.0 9 12937.0 10 15189.0 11 17991.0 12 18415.0 13 20849.0 14 25587.0 15 26319.0 16 20916.0 17 20392.0 18 21471.0 19 22850.0 20 24609.0 21 27126.0 22 30312.0 23 33852.0 24 38442.0 25 43954.0 26 50431.0 27 58694.0 28 68003.0 29 79445.0 30 93748.0 31 112690.0 32 138368.0 33 173688.0 34 225155.0 35 299231.0 36 410757.0 37 478457.0 38 179650.0 39 912.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.921204484202875 18.69818367024067 13.354978114669693 21.025633730886764 2 13.798755056860282 22.622842933718545 38.881901047267064 24.696500962154104 3 22.709006575401304 25.766520962480094 29.983955866916588 21.54051659520201 4 11.199909647411612 15.951602622043303 42.42438652725421 30.424101203290878 5 11.145672039288257 39.49114657423687 35.096954768428965 14.266226618045911 6 26.480332819787318 37.97912356756511 18.943552490989628 16.596991121657943 7 25.70649144853562 33.31113036533848 23.41392136582869 17.568456820297218 8 23.12653485340926 31.498758132275697 20.695138255679307 24.679568758635735 9 22.888558395283404 20.55871831876178 24.99482584059114 31.557897445363675 10-11 24.10282089801369 25.029236699726727 28.744687712196566 22.123254690063018 12-13 28.152513887587787 23.201412318391736 27.56635491543008 21.079718878590395 14-15 22.794066884660026 25.75326644855679 29.57866016403508 21.874006502748102 16-17 19.6448881962842 29.17586957183021 29.85402039190128 21.325221839984305 18-19 18.868657050519747 31.761073492172308 29.325482322985014 20.044787134322927 20-21 21.20701536457937 28.006517999074305 27.45346028708502 23.333006349261304 22-23 19.712977125925406 27.94587838369359 28.545481561765246 23.795662928615755 24-25 22.536666999944764 27.563683501405713 28.284490612395008 21.615158886254516 26-27 21.912899866783178 25.407931723625698 31.9063465290996 20.772821880491524 28-29 19.597317243461887 30.033801109731122 29.91517986488299 20.453701781924003 30-31 20.534998745345316 27.92441645442456 28.759675485971037 22.780909314259084 32-33 21.121248939770947 27.821774162950145 29.45094691065296 21.606029986625945 34-35 23.03993030692337 26.211248570282976 29.425950144340003 21.322870978453658 36-37 22.404526592366043 25.880914972144264 31.373101794463548 20.34145664102614 38-39 21.457153748500566 27.867409710394803 30.76688897359945 19.908547567505185 40-41 20.439729744145314 29.080467307942854 29.793006119841873 20.68679682806995 42-43 19.547182797744693 29.455903447680104 27.606411990069596 23.390501764505604 44-45 20.88497385411804 27.382072544114074 27.65398621987017 24.078967381897716 46-47 22.64941904394609 28.668488094692812 27.890243693503592 20.791849167857507 48-49 22.25567403647761 27.929500723950795 28.551266240816 21.263558998755595 50-51 19.502900756526813 28.705821562875773 31.018840365134004 20.772437315463414 52-53 20.62965211689074 29.99543190682838 27.9220280865179 21.452887889762977 54-55 19.510116617086894 28.12255546995997 28.956786041617637 23.4105418713355 56-57 22.001174481939582 26.882149291453622 28.274279492765146 22.842396733841646 58-59 24.074463019180268 26.55033794601503 27.68037471794112 21.694824316863585 60-61 21.890350611777027 27.408376862623975 30.609856642797702 20.091415882801297 62-63 20.84998614390581 27.934113179374787 29.803960307753908 21.411940368965496 64-65 19.903570081795326 30.85157733143427 28.43061960217195 20.81423298459845 66-67 20.83718833896636 27.991064778780373 27.41652033379978 23.755226548453486 68-69 19.80488222817886 29.016158108516887 28.068160955113648 23.11079870819061 70-71 22.50014467640652 28.16794561775602 27.136311050602473 22.19559865523499 72-73 21.597543956211112 26.378884513559193 30.301539905466303 21.722031624763392 74-75 19.86100801970962 30.501019839885572 28.397570569160706 21.2404015712441 76-77 21.0484776549771 27.594703782834877 28.090810981723184 23.266007580464834 78-79 21.397745781392892 28.064485973455234 28.90442608877106 21.633342156380813 80-81 22.659451184191507 27.614550713538954 28.1088986478233 21.617099454446244 82-83 21.40081982320538 26.873233362208072 30.300587255825697 21.42535955876085 84-85 20.96463508554741 28.567869500879844 29.411516682418387 21.055978731154358 86-87 21.044672111362285 29.27442189464493 28.136437131676693 21.544468862316087 88-89 20.35381698676256 28.658066991966162 27.103178080572054 23.88493794069922 90-91 20.89905935296821 26.76342236473902 28.202775182508056 24.134743099784714 92-93 22.276289669526285 28.489720247308725 28.40554083479812 20.82844924836687 94-95 21.25230268928224 27.812761301563143 28.71255249641862 22.222383512735995 96-97 18.64066448567007 28.349714128995966 30.732986967192698 22.276634418141263 98-99 20.39435925719581 29.578466601528053 27.885218387007367 22.14195575426877 100-101 19.414791435579644 27.66625143900877 28.876016053914277 24.042941071497303 102-103 21.083952776563184 26.956624398775688 29.00696108439003 22.952461740271097 104-105 23.02964755009237 26.817913558798917 28.20707907499714 21.94535981611157 106-107 20.931924737724465 27.767500234763993 30.34053107199796 20.96004395551358 108-109 19.75422887973289 27.785646762332533 30.021067894647935 22.43905646328664 110-111 19.614245129231396 29.8766879857697 28.189760185447167 22.319306699551735 112-113 21.207735973782054 27.267121586787496 26.92009051767858 24.605051921751876 114-115 19.95922309823655 27.504226607365624 28.200141204625513 24.336409089772317 116-117 21.83832335957895 27.067765196723165 27.746736267761978 23.34717517593591 118-119 21.23910587101687 26.30431713564115 29.92036586114679 22.536211132195188 120-121 19.8659643837926 30.162263582041415 27.544355858457216 22.42741617570877 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7459.0 1 9099.5 2 6430.5 3 1962.5 4 1647.5 5 1532.5 6 1658.5 7 1858.0 8 2182.0 9 2387.5 10 2258.0 11 2169.0 12 2217.5 13 2349.0 14 2583.5 15 2832.0 16 3033.5 17 3265.0 18 3558.0 19 3855.0 20 4347.0 21 4934.0 22 5801.5 23 7260.0 24 9184.5 25 11592.0 26 14748.5 27 19090.5 28 24816.5 29 31457.0 30 38230.5 31 44841.0 32 51442.5 33 57389.0 34 63020.5 35 69158.5 36 75033.0 37 80862.0 38 86405.5 39 89626.0 40 91840.0 41 93710.5 42 95099.0 43 97664.0 44 98729.5 45 100715.0 46 110603.5 47 156965.5 48 266933.5 49 264964.5 50 154882.0 51 101287.0 52 75614.5 53 61534.0 54 52747.0 55 45324.0 56 38252.5 57 31261.0 58 24858.0 59 19813.0 60 15748.5 61 11925.0 62 8740.5 63 6183.0 64 4131.5 65 2745.0 66 1860.0 67 1221.0 68 763.5 69 494.5 70 326.0 71 222.0 72 171.5 73 136.0 74 101.5 75 74.0 76 48.0 77 35.0 78 25.5 79 16.0 80 14.0 81 16.0 82 13.0 83 9.5 84 8.0 85 8.0 86 7.0 87 6.0 88 6.5 89 6.5 90 6.0 91 5.0 92 3.0 93 3.5 94 4.5 95 2.0 96 0.5 97 1.5 98 1.5 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1885902549465984 2 0.970065885387268 3 0.9956914433491634 4 0.9639018998234608 5 0.9716241963444103 6 0.9882115507548805 7 0.961443231424414 8 0.9534785309767977 9 0.9479378697958474 10-11 0.936354425014423 12-13 0.932008468900615 14-15 0.9333070613649004 16-17 0.9587075299663197 18-19 0.992540192302498 20-21 0.9791560326372647 22-23 0.9528898357263218 24-25 0.9442671817634678 26-27 0.9477127804353712 28-29 0.9772514303563131 30-31 0.9831037537286919 32-33 0.9730959344706003 34-35 0.9816493301686923 36-37 0.9947564567748781 38-39 0.9811125786167878 40-41 0.9903758715286892 42-43 0.9849390977448815 44-45 0.9636248667644131 46-47 0.9597464039377479 48-49 0.9606467613796523 50-51 0.9477127804353712 52-53 0.9587594736648911 54-55 0.9710355010939343 56-57 1.0201915545086784 58-59 0.9914840037648793 60-61 1.0021324619720182 62-63 0.9692521007763159 64-65 0.9605255594163191 66-67 0.9635209793672703 68-69 0.9760913544215516 70-71 0.9665510284506025 72-73 0.9703429184463155 74-75 0.9773899468858368 76-77 0.9932500895163072 78-79 1.0937957753843661 80-81 1.0612097618139016 82-83 1.0787147882324666 84-85 1.0337834889681974 86-87 1.0104780828758249 88-89 1.0092833778086823 90-91 1.0196028592582025 92-93 1.0031020776786848 94-95 0.9992755585505907 96-97 1.008054043609159 98-99 1.0473927379939068 100-101 1.0344414424834352 102-103 1.0039678059882082 104-105 0.9834500450525012 106-107 0.9864974087020238 108-109 0.9839867966044058 110-111 0.9866186106653572 112-113 0.9915013183310697 114-115 0.9962974531658298 116-117 1.0299050261415321 118-119 1.0253166661010575 120-121 0.9999161974996382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2887742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.078675543652956 #Duplication Level Percentage of deduplicated Percentage of total 1 81.40129631016244 44.83475588300191 2 12.18156490195821 13.418889216978139 3 2.9036237594324636 4.797832528398675 4 1.1549134548481488 2.5444441384232195 5 0.5908917690582257 1.6272768014686565 6 0.3480131545545157 1.1500862174778792 7 0.23053145574295103 0.8888157077430393 8 0.17684983506353946 0.7792523748330583 9 0.12609610597213552 0.6250685857341599 >10 0.7867975231575154 8.057187649403415 >50 0.06373371190211456 2.38921559534627 >100 0.02948236455005984 3.071669112505114 >500 0.0018552984983358388 0.7240152343412259 >1k 0.0029428872732106793 3.688858852253859 >5k 7.037339131590756E-4 2.722236673425276 >10k+ 7.037339131590756E-4 8.680395428666142 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 61891 2.143231632188748 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 45843 1.5875033157394254 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 28322 0.9807662872929784 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 23858 0.826181840344463 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15742 0.5451318019407552 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14250 0.49346513642839285 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 12224 0.4233065142246087 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11270 0.3902703219331921 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 11230 0.3888851566379545 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 11178 0.38708444175414564 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10535 0.3648179096332013 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9992 0.34601429075035095 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9168 0.31747988566845653 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8449 0.2925815394865608 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7925 0.2744358741189483 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7088 0.24545129031610163 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6550 0.226820817095156 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6049 0.20947162177230513 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5749 0.1990828820580232 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5667 0.1962432932027861 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5592 0.19364610827421563 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5026 0.17404601934660366 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4736 0.1640035709561311 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4441 0.15378797690375387 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4430 0.15340705644756353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4282 0.14828194485518442 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4069 0.14090593965804424 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3399 0.11770442096281455 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3347 0.11590370607900567 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3163 0.10953194572091274 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3162 0.1094973165885318 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.4629132380939845E-5 0.0 4 0.0 0.0 0.0 3.4629132380939845E-5 0.0 5 0.0 0.0 0.0 3.4629132380939845E-5 0.0 6 0.0 0.0 0.0 3.4629132380939845E-5 0.0 7 0.0 0.0 0.0 3.4629132380939845E-5 0.0 8 0.0 0.0 0.0 6.925826476187969E-5 0.0 9 0.0 0.0 0.0 1.0388739714281954E-4 0.0 10-11 0.0 0.0 0.0 1.7314566190469925E-4 0.0 12-13 0.0 0.0 0.0 3.462913238093985E-4 0.0 14-15 0.0 0.0 0.0 4.8480785333315785E-4 0.0 16-17 0.0 1.7314566190469923E-5 0.0 6.579535152378571E-4 0.0 18-19 0.0 3.4629132380939845E-5 0.0 9.349865742853758E-4 0.0 20-21 0.0 3.4629132380939845E-5 0.0 0.0014197944276185337 0.0 22-23 0.0 5.194369857140977E-5 0.0 0.002077747942856391 0.0 24-25 0.0 6.925826476187969E-5 0.0 0.002995419950951297 0.0 26-27 0.0 6.925826476187969E-5 0.0 0.004259383282855601 0.0 28-29 0.0 6.925826476187969E-5 0.0 0.007341376064759248 0.0 30-31 0.0 6.925826476187969E-5 0.0 0.014561550166185206 0.0 32-33 0.0 6.925826476187969E-5 0.0 0.027911080699037517 0.0 34-35 0.0 6.925826476187969E-5 0.0 0.039684985708557066 0.0 36-37 0.0 6.925826476187969E-5 0.0 0.044498435109507704 0.0 38-39 0.0 6.925826476187969E-5 0.0 0.04789209008283981 0.0 40-41 0.0 6.925826476187969E-5 0.0 0.054177277609980395 0.0 42-43 0.0 1.0388739714281954E-4 0.0 0.06075681276235896 0.0 44-45 0.0 1.0388739714281954E-4 0.0 0.06773458293711834 0.0 46-47 0.0 1.0388739714281954E-4 0.0 0.07504132986949665 0.0 48-49 0.0 1.0388739714281954E-4 0.0 0.0834735236042555 0.0 50-51 0.0 1.0388739714281954E-4 0.0 0.09647676281329842 0.0 52-53 0.0 1.0388739714281954E-4 0.0 0.12850871026566776 0.0 54-55 1.7314566190469923E-5 1.0388739714281954E-4 0.0 0.16519827602327355 0.0 56-57 3.4629132380939845E-5 1.0388739714281954E-4 0.0 0.2091426450146862 0.0 58-59 3.4629132380939845E-5 1.0388739714281954E-4 0.0 0.29381087368608416 0.0 60-61 3.4629132380939845E-5 1.0388739714281954E-4 0.0 0.4082255270727094 0.0 62-63 3.4629132380939845E-5 1.0388739714281954E-4 0.0 0.4584204544588817 0.0 64-65 3.4629132380939845E-5 1.0388739714281954E-4 0.0 0.5122341261788622 0.0 66-67 3.4629132380939845E-5 1.0388739714281954E-4 0.0 0.7079926115283152 0.0 68-69 3.4629132380939845E-5 1.0388739714281954E-4 0.0 0.9043397921282441 0.0 70-71 3.4629132380939845E-5 1.0388739714281954E-4 0.0 1.0473234797291449 0.0 72-73 3.4629132380939845E-5 1.0388739714281954E-4 0.0 1.1131188312529305 0.0 74-75 3.4629132380939845E-5 1.0388739714281954E-4 0.0 1.4044883511061583 0.0 76-77 3.4629132380939845E-5 1.0388739714281954E-4 0.0 1.4715649805280389 0.0 78-79 3.4629132380939845E-5 1.3851652952375938E-4 0.0 1.519837991067069 0.0 80-81 3.4629132380939845E-5 1.3851652952375938E-4 0.0 1.6175094589475099 0.0 82-83 3.4629132380939845E-5 1.3851652952375938E-4 0.0 1.864345914558849 0.0 84-85 5.194369857140977E-5 1.3851652952375938E-4 0.0 2.096759336533527 0.0 86-87 6.925826476187969E-5 1.3851652952375938E-4 0.0 2.5803724847995424 0.0 88-89 6.925826476187969E-5 1.3851652952375938E-4 0.0 3.254480490293108 0.0 90-91 6.925826476187969E-5 1.3851652952375938E-4 0.0 3.9939856122880784 0.0 92-93 6.925826476187969E-5 1.3851652952375938E-4 0.0 4.7318804796273355 0.0 94-95 6.925826476187969E-5 1.3851652952375938E-4 0.0 5.378008146157102 0.0 96-97 6.925826476187969E-5 1.3851652952375938E-4 0.0 6.067318340765899 0.0 98-99 6.925826476187969E-5 1.3851652952375938E-4 0.0 6.782825474020878 0.0 100-101 6.925826476187969E-5 1.3851652952375938E-4 0.0 7.56289862460012 0.0 102-103 6.925826476187969E-5 1.3851652952375938E-4 0.0 8.32447981848794 0.0 104-105 6.925826476187969E-5 1.3851652952375938E-4 0.0 9.032472430016256 0.0 106-107 6.925826476187969E-5 1.3851652952375938E-4 0.0 9.725886176812194 0.0 108-109 6.925826476187969E-5 1.3851652952375938E-4 0.0 10.543358790362852 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4225 0.0 60.318676 2 ACCTGGG 4680 0.0 53.165554 3 GTACCTG 5670 0.0 45.792614 1 TATAACG 490 0.0 41.091007 2 GTACCGG 315 0.0 36.552864 1 TACACGG 500 0.0 34.51644 2 CCTGGGG 2945 0.0 31.802425 4 ATAACGC 655 0.0 31.58272 3 GTACCCG 495 0.0 30.239187 1 AGTACGC 1185 0.0 30.098726 2 GTACGCA 1205 0.0 30.0429 3 GTACACG 595 0.0 29.994848 1 GAGTACG 1385 0.0 27.018766 1 TACGCAG 1360 0.0 26.194538 4 TACCGGG 405 0.0 25.567736 2 TACCCGG 595 0.0 25.138023 2 ACACGGG 665 0.0 25.059053 3 TAACGCA 740 0.0 24.847128 4 GTATAAC 1070 0.0 24.212 1 CCTGGGA 3015 0.0 23.822132 4 >>END_MODULE