FastQCFastQC Report
Wed 25 May 2016
SRR1003829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435462
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT112810.7858793893533929No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT70370.4902254465809614No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC46310.32261390409498825No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46290.322474576129497No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37640.26221523105453154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.2519049616081791No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33120.2307271108535092No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28470.19833335887679365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27230.18969502501633623No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25340.17652853227741314No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23860.16621826283106067No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21270.14817529129994383No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20540.14308982055951325No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18310.1275547524072389No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA17190.11975238633972894No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16770.11682649906441271No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA16760.11675683508166709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15830.110278084686324No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15520.10811850122120963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17700.048.7806432
ACCTGGG18850.044.4967583
GTACCTG23650.037.7557371
TAACGCA2800.034.8801044
GTACCGG1102.5444442E-531.4225161
TATAACG3150.031.0647532
CCTGGGG14400.030.3206144
GTACCCG2554.3655746E-1127.1096231
ATAACGC3900.025.0421243
TAACGCC1401.3400851E-424.621254
AGTACGC2452.3050234E-823.4943522
GTACACG2002.4187684E-623.0431791
TACCCGG3003.3833203E-1023.0244622
CTGGGGG10650.022.657775
AGTACTT45400.022.65202512-13
GTATAAC6650.022.5234071
GAGTACG3657.2759576E-1222.09621
GTGCTAC3458.185452E-1121.7073421
GAGTACT42750.021.16675412-13
GTACATG51400.020.3981461
Produced by FastQC (version 0.11.5)