FastQCFastQC Report
Wed 25 May 2016
SRR1003829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435462
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129760.9039598401072269No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT67820.4724611309808271No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52970.3690101166035743No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52710.3671988530521881No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46540.3242161756981376No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43940.30610354018427516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.3058248842532927No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT43230.3011573974093358No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29060.20244353385878555No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA26750.18635115384454623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26050.18147467505235249No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24470.17046776577854378No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22860.1592518645564982No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC21920.15270345017840944No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG18210.1268581125797827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2700.052.7721442
TAACGCA3000.047.485014
ATAACGC3150.046.7410473
TACCTGG17250.044.6039352
ACCTGGG18700.040.6373753
GTACCTG22050.035.7857441
GTACCCG1900.035.025551
TATCACG2400.031.6632862
GTACACG1901.2732926E-1130.02191
CCTGGGG14100.028.9624844
TACCCGG2151.8189894E-1228.7178672
GTATAAC5600.027.1626741
GGACGGT908.9651486E-426.3805586
TAACCGT2007.221388E-1026.1167537
TACCGCG2501.4551915E-1124.6922048
GTACCGG1000.001646834723.767341
TCACGCA3200.023.7425024
ACACGGG2303.6325218E-922.7149683
TACACGG2303.6325218E-922.7149682
AACCGTG2354.669346E-922.2270248
Produced by FastQC (version 0.11.5)