##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003829_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1435462 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.679156954346407 31.0 31.0 34.0 30.0 34.0 2 31.863971320731583 31.0 31.0 34.0 30.0 34.0 3 31.906956088005117 31.0 31.0 34.0 30.0 34.0 4 35.713189899837126 37.0 35.0 37.0 33.0 37.0 5 35.55082126869259 37.0 35.0 37.0 33.0 37.0 6 35.59741393363252 37.0 35.0 37.0 33.0 37.0 7 35.53417297009604 37.0 35.0 37.0 33.0 37.0 8 35.53000427736854 37.0 35.0 37.0 33.0 37.0 9 37.115137147482834 39.0 37.0 39.0 33.0 39.0 10-11 37.03388456120747 39.0 37.0 39.0 33.0 39.0 12-13 36.9910028966284 39.0 37.0 39.0 33.0 39.0 14-15 38.14572102918781 40.0 37.0 41.0 33.0 41.0 16-17 38.13404952551861 40.0 37.0 41.0 33.0 41.0 18-19 38.15212175592249 40.0 37.0 41.0 33.0 41.0 20-21 38.112167720218295 40.0 37.0 41.0 33.0 41.0 22-23 38.027899728449796 40.0 37.0 41.0 33.0 41.0 24-25 37.91956735880156 40.0 37.0 41.0 33.0 41.0 26-27 37.7525016336204 40.0 37.0 41.0 32.0 41.0 28-29 37.59417560339459 39.5 37.0 41.0 32.0 41.0 30-31 37.37145288415855 39.0 36.0 41.0 32.0 41.0 32-33 37.113327625531014 39.0 36.0 40.5 31.0 41.0 34-35 37.0767373152337 39.0 36.0 41.0 31.0 41.0 36-37 36.923237954052425 39.0 35.5 41.0 31.0 41.0 38-39 36.69064071358211 39.0 35.0 40.0 30.0 41.0 40-41 36.67888805137301 39.0 35.0 41.0 30.0 41.0 42-43 36.53358709600114 39.0 35.0 41.0 30.0 41.0 44-45 36.24272115876282 39.0 35.0 41.0 30.0 41.0 46-47 36.0589231898859 39.0 35.0 41.0 29.5 41.0 48-49 35.82135995240557 39.0 35.0 41.0 28.0 41.0 50-51 35.608206974479295 39.0 35.0 40.0 27.5 41.0 52-53 35.409228875442196 39.0 35.0 40.0 27.0 41.0 54-55 35.217225186037666 38.0 34.5 40.0 26.5 41.0 56-57 34.96605552776737 38.0 34.0 40.0 26.0 41.0 58-59 34.705737246963 38.0 34.0 40.0 26.0 41.0 60-61 34.40019450183983 38.0 34.0 40.0 25.0 41.0 62-63 34.13468381608151 37.5 33.0 40.0 24.5 41.0 64-65 33.68490005308395 37.0 33.0 40.0 23.0 41.0 66-67 33.36924279430595 36.5 32.0 39.0 22.0 41.0 68-69 33.08798108204884 36.0 32.0 39.0 22.0 41.0 70-71 32.69997046247131 35.5 32.0 39.0 21.5 40.0 72-73 32.32124849003317 35.0 31.5 38.0 20.5 40.0 74-75 31.93115038921267 35.0 31.0 37.5 20.0 39.5 76-77 30.887558152009596 34.0 30.0 36.0 19.0 39.0 78-79 31.310232176121694 35.0 31.0 36.0 19.0 39.0 80-81 31.12186982309528 35.0 31.0 36.0 18.0 38.5 82-83 30.807485325282038 34.5 31.0 36.0 17.5 37.0 84-85 30.443492408715798 34.0 31.0 35.0 14.0 37.0 86-87 30.1099196634951 34.0 30.0 35.0 9.0 36.5 88-89 29.800005155134723 34.0 30.0 35.0 7.0 36.0 90-91 29.521968885278746 34.0 30.0 35.0 2.0 36.0 92-93 29.250176946516174 34.0 30.0 35.0 2.0 35.5 94-95 28.887282979277753 34.0 29.0 35.0 2.0 35.0 96-97 28.539754448393616 34.0 29.0 35.0 2.0 35.0 98-99 28.16123206326604 34.0 29.0 35.0 2.0 35.0 100-101 27.290131678860185 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.061285814649131964 1101 2 0.1914038126311901 1101 3 0.23462179674163863 1101 4 0.1761901064983249 1101 5 0.2714162422570183 1101 6 0.23422237705455728 1101 7 0.15253250798016182 1101 8 0.15428250894562012 1101 9 0.22455603849680728 1101 10-11 0.349543149084937 1101 12-13 0.37245935898941696 1101 14-15 0.4569223395667521 1101 16-17 0.414806981439952 1101 18-19 0.3226633488102877 1101 20-21 0.2859374043825298 1101 22-23 0.18270677110594136 1101 24-25 0.2762622959749237 1101 26-27 0.36646700698822343 1101 28-29 0.3855507849348072 1101 30-31 0.3755575252185679 1101 32-33 0.3806396021055676 1101 34-35 0.39056794427004604 1101 36-37 0.37803954777341175 1101 38-39 0.2646064014667644 1101 40-41 0.31355985571337186 1101 42-43 0.3340134652819202 1101 44-45 0.4648466669104252 1101 46-47 0.40762081234781533 1101 48-49 0.35071297915003896 1101 50-51 0.20969575735023938 1101 52-53 0.06507378879370407 1101 54-55 0.217671604420957 1101 56-57 0.11021925004192923 1101 58-59 0.20701106488632348 1101 60-61 0.2485201910182937 1101 62-63 -0.0093756505508793 1101 64-65 0.05084827913936607 1101 66-67 -0.046047985138677916 1101 68-69 0.1002129025331655 1101 70-71 0.23211258906682275 1101 72-73 0.1273007305490239 1101 74-75 0.08334324991179898 1101 76-77 0.14122092798123376 1101 78-79 0.02085420448867481 1101 80-81 0.08012477033857479 1101 82-83 0.2564251425624704 1101 84-85 0.1840121158003356 1101 86-87 0.011050473377306247 1101 88-89 0.022457865642625308 1101 90-91 0.1442607103440814 1101 92-93 0.30021588813098177 1101 94-95 0.2499366586117091 1101 96-97 0.11094638936193846 1101 98-99 0.1273861549154809 1101 100-101 0.2953316452635555 1102 1 0.061751547615724434 1102 2 0.16839428400966838 1102 3 0.15198856660854076 1102 4 0.034398048534335146 1102 5 0.05592795733155498 1102 6 0.005545771886978912 1102 7 -0.022288352388450505 1102 8 0.03420116894078973 1102 9 0.13973936285060518 1102 10-11 0.21836735379434202 1102 12-13 0.041908393452800397 1102 14-15 0.14302347580052555 1102 16-17 0.14638797300434447 1102 18-19 0.09664064819495621 1102 20-21 0.09972799812332056 1102 22-23 0.0760786262269022 1102 24-25 0.1384708584153671 1102 26-27 -0.008340835198758612 1102 28-29 -0.03108666601071519 1102 30-31 0.021540320595953233 1102 32-33 0.02342542423166094 1102 34-35 0.12715749861859393 1102 36-37 0.06864613488738769 1102 38-39 0.08688115470133084 1102 40-41 0.1052962319499926 1102 42-43 0.13182843008986111 1102 44-45 0.34564695425563485 1102 46-47 0.2571162396578188 1102 48-49 0.2122639730431004 1102 50-51 0.43651071958031906 1102 52-53 0.24523188880943536 1102 54-55 0.19639332209913363 1102 56-57 0.3354251993938959 1102 58-59 0.3425739444221776 1102 60-61 0.1843199327169529 1102 62-63 0.11484422524792848 1102 64-65 0.0735020633915795 1102 66-67 -0.06489169563837294 1102 68-69 0.01004008294970049 1102 70-71 0.03132518241749693 1102 72-73 0.08460173926476244 1102 74-75 0.0716445018054479 1102 76-77 -0.12980708731749502 1102 78-79 -0.018888843243797027 1102 80-81 0.12102076494913305 1102 82-83 0.013145188070254221 1102 84-85 0.06685986666241561 1102 86-87 0.09692578927229079 1102 88-89 -0.11020750178373717 1102 90-91 -0.1315960621608312 1102 92-93 -0.10562699831352518 1102 94-95 0.03911343692539404 1102 96-97 0.2393754772213441 1102 98-99 0.1053089515130523 1102 100-101 0.28893791358245835 1103 1 -0.09479021319164715 1103 2 -0.019255909541467275 1103 3 6.512028389558111E-4 1103 4 0.026663436853269218 1103 5 0.13151746934283182 1103 6 0.019624380757079507 1103 7 0.058180452203451694 1103 8 0.12614056074766467 1103 9 0.049192815673038126 1103 10-11 0.16089696382262275 1103 12-13 0.029145813372288387 1103 14-15 0.09890776735776541 1103 16-17 0.06220524645832626 1103 18-19 -0.10208254865232647 1103 20-21 -0.11336422102682064 1103 22-23 -0.0726219718959129 1103 24-25 -0.01491104565602086 1103 26-27 -0.12576016493843412 1103 28-29 -0.15707368760289597 1103 30-31 -0.11590019049203448 1103 32-33 -0.03858379069558282 1103 34-35 -0.12582246037661093 1103 36-37 -0.1883647972455691 1103 38-39 -0.07160825933257797 1103 40-41 0.04211594715650335 1103 42-43 0.14186553461838258 1103 44-45 0.1814549552943845 1103 46-47 0.1667442626708464 1103 48-49 0.2194353511638596 1103 50-51 0.1291481195772448 1103 52-53 0.085454446598483 1103 54-55 0.07944888770407488 1103 56-57 -0.13058379152845845 1103 58-59 -0.1853325067702798 1103 60-61 -0.06624937177261359 1103 62-63 -0.08061392672155421 1103 64-65 -0.016328733614891178 1103 66-67 -0.044136179641604656 1103 68-69 -0.00818943088533075 1103 70-71 -0.18144651644656307 1103 72-73 0.08644556721115748 1103 74-75 -0.21938085584661948 1103 76-77 -0.06585913971283475 1103 78-79 0.10390325889847318 1103 80-81 0.10840624120486098 1103 82-83 0.18250144159820536 1103 84-85 0.11118223449332731 1103 86-87 0.1304689124457461 1103 88-89 0.019013094971267464 1103 90-91 0.31762767660980984 1103 92-93 0.2626016234665869 1103 94-95 0.11298772557355008 1103 96-97 0.27979213129186675 1103 98-99 0.377804841865629 1103 100-101 0.48345494761881014 1104 1 -0.07662089490724355 1104 2 -0.07982142968821648 1104 3 0.02138657308798031 1104 4 0.008631678851287461 1104 5 -0.1688810256002995 1104 6 -0.0022790236756762283 1104 7 -0.10945302579334992 1104 8 -0.2542126989704485 1104 9 0.02010372880924649 1104 10-11 -0.04411821386656811 1104 12-13 -0.09800072578909891 1104 14-15 -0.14440965852087828 1104 16-17 -0.10438424725228401 1104 18-19 -0.26389443250968725 1104 20-21 -0.29938149466799757 1104 22-23 -0.20957382641117306 1104 24-25 -0.08990780623418715 1104 26-27 -0.016050005681698565 1104 28-29 -0.11166958277950556 1104 30-31 -0.06424461564665762 1104 32-33 -0.05178328319640002 1104 34-35 -0.1504466720917108 1104 36-37 -0.10168846246560292 1104 38-39 -0.010736913200815934 1104 40-41 0.12022101741971625 1104 42-43 0.005361211602270544 1104 44-45 0.14409275354380213 1104 46-47 7.235868691140013E-4 1104 48-49 0.04176411778240663 1104 50-51 -0.02585345561080743 1104 52-53 -0.035269286296291114 1104 54-55 -0.0392505903651994 1104 56-57 0.009787265051194538 1104 58-59 -0.08434268684528945 1104 60-61 -0.029354660044312197 1104 62-63 -0.04077548636038131 1104 64-65 -0.01838937436330923 1104 66-67 0.010367476452778135 1104 68-69 0.07829714439791502 1104 70-71 -0.058109291712987954 1104 72-73 -0.07806916125774421 1104 74-75 -0.06193712506437521 1104 76-77 -0.1114221450856725 1104 78-79 -0.0065912703168145015 1104 80-81 -5.447265378677457E-4 1104 82-83 -0.10365250186820418 1104 84-85 -0.08908342880208409 1104 86-87 -0.0010189125480977168 1104 88-89 -0.03238516845299344 1104 90-91 0.04082921409160889 1104 92-93 0.06958520282198322 1104 94-95 0.14665529764070584 1104 96-97 0.22972203081758735 1104 98-99 0.1698771015450511 1104 100-101 0.2668219136223833 1105 1 -0.05309985355219027 1105 2 -0.022286562889494377 1105 3 0.012205184884077624 1105 4 -0.11687262644038299 1105 5 -0.10996118891840467 1105 6 0.004585996334832032 1105 7 -0.12249583615839299 1105 8 0.0034935709895265177 1105 9 0.10528139788795698 1105 10-11 0.06398018125005223 1105 12-13 0.030436433122652318 1105 14-15 0.15979214444633527 1105 16-17 0.04664700148443046 1105 18-19 0.07417889893712726 1105 20-21 -0.025554029736923667 1105 22-23 0.21846110102545424 1105 24-25 0.17062823289747797 1105 26-27 0.14027169864784383 1105 28-29 0.049185411763083664 1105 30-31 0.21816675194035895 1105 32-33 0.28771342214377427 1105 34-35 0.23333470802713663 1105 36-37 0.10572472425873514 1105 38-39 1.3303869311442895E-4 1105 40-41 0.15971570149620362 1105 42-43 0.2120447221330508 1105 44-45 0.24726735030169777 1105 46-47 0.050494375566209726 1105 48-49 -0.07530787963037966 1105 50-51 -0.07354800224135971 1105 52-53 -0.06127122670943663 1105 54-55 0.1049233191630492 1105 56-57 -0.10394843450817604 1105 58-59 -0.03562133825814584 1105 60-61 0.026004922766865946 1105 62-63 -0.01777835655482818 1105 64-65 -0.04531664929560009 1105 66-67 -0.04109793972541098 1105 68-69 -0.21999286651691108 1105 70-71 -0.13062536075934617 1105 72-73 -0.13009568023740314 1105 74-75 0.04254522930357396 1105 76-77 -0.21891092707216941 1105 78-79 -0.07194074680952056 1105 80-81 0.012989250904830385 1105 82-83 0.12845527191196382 1105 84-85 0.06755278415825572 1105 86-87 0.043859205251148836 1105 88-89 0.1256408654233887 1105 90-91 0.12318519905340608 1105 92-93 0.17401055755376404 1105 94-95 -0.07404122982826777 1105 96-97 -0.09626899477160933 1105 98-99 -0.2740930646028268 1105 100-101 -0.19316595879545062 1106 1 -0.06444479569905681 1106 2 -0.09120396465372593 1106 3 -0.03868380583650932 1106 4 -0.11831036272174345 1106 5 -0.028938162225770725 1106 6 -0.07912233222513976 1106 7 -0.039367184537361766 1106 8 -0.017552367835079963 1106 9 -0.2770281450150094 1106 10-11 -0.22126268734886878 1106 12-13 -0.21587471362955313 1106 14-15 -0.12287826453522399 1106 16-17 -0.07867033154341385 1106 18-19 -0.3317685177199863 1106 20-21 -0.22494857664951695 1106 22-23 -0.3675997306849368 1106 24-25 -0.01446857086011022 1106 26-27 -0.08234085720047091 1106 28-29 -0.21880805827930772 1106 30-31 -0.13247994298460952 1106 32-33 -0.11364526728227986 1106 34-35 -0.039428495088912996 1106 36-37 -0.07586938732335113 1106 38-39 0.04937922955117102 1106 40-41 -0.015322676324849738 1106 42-43 0.13889373118335158 1106 44-45 0.18760587705160958 1106 46-47 0.022377180011162068 1106 48-49 0.045028928709641036 1106 50-51 -0.037983790991496846 1106 52-53 0.1930328172818534 1106 54-55 0.30326390800483694 1106 56-57 0.011317919026019752 1106 58-59 0.15128552392537387 1106 60-61 0.09016633873662272 1106 62-63 0.06604166864851635 1106 64-65 0.0653861803526965 1106 66-67 -0.2564633882303937 1106 68-69 -0.29503296274786095 1106 70-71 -0.26655330210429184 1106 72-73 -0.20517186307622026 1106 74-75 -0.1486834337735239 1106 76-77 -0.1769072611310527 1106 78-79 -0.11650770751521478 1106 80-81 0.04997172312386766 1106 82-83 0.0794748617848029 1106 84-85 0.1231210050312086 1106 86-87 0.16743578905227352 1106 88-89 0.223013989492582 1106 90-91 0.3155090620388563 1106 92-93 0.11581202393021073 1106 94-95 0.26374545822305606 1106 96-97 0.305573140639094 1106 98-99 0.2509905646788795 1106 100-101 0.16115469649152203 1107 1 -0.0990809973227833 1107 2 0.08703193774665863 1107 3 0.1325940538365451 1107 4 0.037236269915602804 1107 5 -0.01077024512823499 1107 6 -0.06257154126344489 1107 7 0.021231042124320254 1107 8 -0.02019807420017372 1107 9 -0.0996173069696411 1107 10-11 -0.02430057430404986 1107 12-13 -0.07729690456044125 1107 14-15 0.013174847607523077 1107 16-17 -0.1279734271848696 1107 18-19 -0.011448282741660876 1107 20-21 -0.08262772562313359 1107 22-23 -0.08853821388662197 1107 24-25 -0.09189811036419115 1107 26-27 -0.027971866419200353 1107 28-29 -0.1197810841753224 1107 30-31 0.15044287499519982 1107 32-33 -0.02671648683740102 1107 34-35 -0.14223701415340173 1107 36-37 -0.024069096900696252 1107 38-39 -0.09902234577170788 1107 40-41 0.09252423573428104 1107 42-43 0.10628443765845219 1107 44-45 0.22005565813454098 1107 46-47 -0.052013372451924056 1107 48-49 -0.014793737458326461 1107 50-51 -0.1493454772030205 1107 52-53 0.19035960877159397 1107 54-55 0.143413189648804 1107 56-57 0.15898243685841607 1107 58-59 0.17228066154610389 1107 60-61 -0.19978602754795105 1107 62-63 -0.20871301221659166 1107 64-65 0.0742116187368893 1107 66-67 -0.026812225439698523 1107 68-69 0.028252003479501298 1107 70-71 0.1297168569681162 1107 72-73 0.063315078905255 1107 74-75 -0.10929211125036531 1107 76-77 0.103400102843338 1107 78-79 -0.024565286006332343 1107 80-81 0.030907236093881352 1107 82-83 0.07534954000970373 1107 84-85 0.10588541065484591 1107 86-87 0.05931935597049076 1107 88-89 0.04352948970160142 1107 90-91 0.11627527180321806 1107 92-93 0.2392168995105024 1107 94-95 0.05566972831612205 1107 96-97 0.1293943986972188 1107 98-99 0.12399390893973106 1107 100-101 0.33624160327025265 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78-79 -0.5362989698224432 2316 80-81 -0.5186349445846794 2316 82-83 -0.6257805085286208 2316 84-85 -0.5869331140155722 2316 86-87 -0.5292505221309831 2316 88-89 -0.6109385914339178 2316 90-91 -0.6824094846366151 2316 92-93 -0.6553421712411733 2316 94-95 -0.7813898772041235 2316 96-97 -0.9819699000094282 2316 98-99 -0.9690870471259778 2316 100-101 -1.3274650535576313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 11.0 5 27.0 6 87.0 7 166.0 8 499.0 9 881.0 10 1526.0 11 2631.0 12 4022.0 13 5476.0 14 6614.0 15 8775.0 16 10921.0 17 14071.0 18 12512.0 19 8504.0 20 7633.0 21 7716.0 22 8203.0 23 8860.0 24 10465.0 25 12718.0 26 15754.0 27 19546.0 28 24948.0 29 31119.0 30 38606.0 31 47450.0 32 58088.0 33 73235.0 34 94395.0 35 126482.0 36 178451.0 37 260228.0 38 272683.0 39 62130.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68098514475326 20.753915971307276 15.15465211117385 21.410446772765614 2 15.320291763328944 21.405312767780636 40.24411143854369 23.03028403034673 3 19.540430940943708 26.714951639531048 30.738428366643365 23.00618905288187 4 12.46525508860562 17.61753358848928 40.02822784580853 29.88898347709657 5 12.258771043747588 38.014172440649766 35.70864293168332 14.018413583919322 6 28.50280954842413 38.771698589025696 18.235453115442972 14.490038747107203 7 26.0241650423348 33.53777390136416 22.818089228415662 17.61997182788538 8 24.668294946156706 34.55918721638051 20.83148143245868 19.941036405004102 9 24.254212232716714 18.497947002428486 23.002002142864107 34.245838621990686 10-11 23.964410064494913 26.77016180156632 28.61691915216146 20.648508981777294 12-13 25.622099365918427 24.627297692310908 28.82974261944935 20.920860322321317 14-15 21.929407486438784 26.476877574784798 28.391662321295524 23.2020526174809 16-17 20.048562762371976 29.830605059555737 28.88164228659484 21.239189891477448 18-19 19.870989270353377 29.260475024765547 31.125310178883176 19.7432255259979 20-21 20.982925261659958 27.30446263257177 30.604731041954423 21.107881063813853 22-23 20.355432606366453 27.48575023232938 30.959649227914078 21.199167933390086 24-25 21.454904414049274 27.401108493293446 30.819798782552237 20.32418831010504 26-27 20.872646027951927 26.889604449791797 32.07445626389428 20.163293258361996 28-29 20.20840293277382 28.318016521325035 31.48558503417238 19.987995511728762 30-31 20.636988071757564 27.82601668044976 30.749171098274758 20.787824149517917 32-33 20.716215251229194 27.786023683417604 30.929519277828188 20.568241787525015 34-35 21.414889247985656 27.130995272021607 31.0704083171734 20.38370716281934 36-37 21.420157882029883 27.12349727613608 31.489587931316194 19.966756910517837 38-39 20.978254847211055 27.733249980921638 31.4173777930637 19.871117378803607 40-41 20.361251722518773 28.16856370149603 31.06284807419266 20.407336501792532 42-43 20.26342988341742 28.41261044028413 29.95974109171068 21.364218584587775 44-45 20.919922290703415 27.521102003379667 30.05929914630548 21.499676559611437 46-47 21.588500846448053 27.969952835775146 29.978055050473383 20.463491267303418 48-49 21.550059093029112 28.056527280498045 29.831826080838148 20.56158754563469 50-51 20.46669961820109 28.33953874121655 30.6224948909447 20.571266749637655 52-53 20.8436088423716 29.304776474587797 28.868607240088835 20.983007442951767 54-55 20.445582456196572 28.478237092696563 29.456503792895173 21.61967665821169 56-57 21.604923864110212 27.64249591808795 29.35862336362094 21.3939568541809 58-59 22.131496177371897 27.768126523817273 29.168009268919544 20.93236802989129 60-61 21.200210255200908 27.924126993305343 30.40159231042808 20.47407044106567 62-63 20.414126879129277 28.419007729871453 29.987797873144554 21.179067517854712 64-65 20.351228721861546 29.075718660062915 29.732503402271153 20.840549215804387 66-67 20.648879671577532 27.772702216963154 29.05763212195841 22.520785989500904 68-69 20.500020556044486 28.409603226532486 29.34981579693694 21.740560420486084 70-71 21.51720798336161 28.045546548126264 29.260330189829542 21.176915278682586 72-73 20.975590386071634 27.528267961676367 30.27717963383141 21.21896201842059 74-75 20.52041926688207 28.947659910634794 29.455337599567528 21.076583222915612 76-77 20.555279994649784 28.422327121137812 29.01200036782737 22.01039251638504 78-79 20.707078447545587 28.21800180569798 29.384495240536808 21.690424506219628 80-81 21.103378371317806 28.163752956379685 29.04761904761905 21.68524962468346 82-83 20.95344615230282 27.98222307057827 29.904539045432255 21.159791731686656 84-85 20.57693942125627 28.215150050088685 29.25440313656864 21.953507392086404 86-87 20.44757491599175 28.427235288237036 28.816492960793134 22.308696834978083 88-89 20.450183530146106 28.24114098695957 28.588590827045568 22.72008465584876 90-91 20.65090480463242 26.954233103532832 29.270792115074872 23.12406997675987 92-93 20.824684813029993 27.60747009228765 29.408986333625343 22.158858761057015 94-95 20.478152373972616 28.023667971259027 29.473291153526233 22.024888501242128 96-97 19.713879658076785 28.33911565336733 30.051831880786672 21.89517280776921 98-99 20.106061545514017 29.083881557176326 28.12431856641352 22.68573833089614 100-101 19.175210974800066 28.014833898928558 28.68532724520173 24.124627881069642 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 221.0 1 173.5 2 312.5 3 492.5 4 355.5 5 241.0 6 366.0 7 696.0 8 1057.0 9 1310.0 10 1355.0 11 1289.5 12 1347.5 13 1503.5 14 1687.5 15 1835.0 16 2002.0 17 2199.0 18 2401.5 19 2611.0 20 2832.0 21 3096.0 22 3540.0 23 4195.5 24 5172.0 25 6396.0 26 7861.5 27 9997.5 28 13092.0 29 16820.0 30 20767.5 31 24997.5 32 29406.0 33 33173.5 34 36764.0 35 40513.0 36 43963.0 37 48076.0 38 51724.0 39 52973.0 40 53953.5 41 55081.5 42 55493.0 43 56743.5 44 56881.0 45 57172.5 46 58934.0 47 67791.5 48 85064.5 49 80235.0 50 60091.0 51 48757.5 52 39893.5 53 33492.5 54 29135.0 55 25145.0 56 20829.0 57 16830.5 58 13509.5 59 11065.0 60 8800.0 61 6649.5 62 5092.5 63 3671.0 64 2418.5 65 1607.0 66 1024.0 67 618.5 68 335.0 69 184.5 70 101.0 71 61.5 72 39.0 73 18.5 74 8.0 75 8.0 76 5.5 77 6.0 78 5.0 79 2.0 80 1.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06632011157383477 2 0.0036225271027724875 3 0.035458967217523 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0018460955427590559 16-17 0.0 18-19 0.0 20-21 0.010763085334199025 22-23 0.0 24-25 0.0 26-27 0.004806814809448109 28-29 0.011529389144400897 30-31 0.008986653774185593 32-33 0.004841646800820921 34-35 0.0049113107835665444 36-37 0.019262091229165244 38-39 0.04113658181129142 40-41 0.004458494895719984 42-43 0.008882157800067157 44-45 0.007837198058882784 46-47 0.0036225271027724875 48-49 9.404637670659342E-4 50-51 0.0014281116462853075 52-53 6.966398274562475E-4 54-55 0.0010449597411843713 56-57 0.01159905312714652 58-59 0.008882157800067157 60-61 0.004423662904347172 62-63 0.0039360150251277985 64-65 0.07844164457157347 66-67 0.03350837570064551 68-69 0.025148697771170535 70-71 0.01581372408325682 72-73 2.0899194823687425E-4 74-75 2.78655930982499E-4 76-77 4.179838964737485E-4 78-79 4.179838964737485E-4 80-81 4.8764787921937327E-4 82-83 4.179838964737485E-4 84-85 5.57311861964998E-4 86-87 0.0026123993529609283 88-89 2.0899194823687425E-4 90-91 9.752957584387465E-4 92-93 3.1348792235531137E-4 94-95 0.001393279654912495 96-97 0.0036225271027724875 98-99 0.004806814809448109 100-101 0.0070360622573081 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1435462.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.12958590990985 #Duplication Level Percentage of deduplicated Percentage of total 1 82.7055511920963 51.38461648015794 2 10.78710430655715 13.40396647466802 3 2.805901339824834 5.229884650421345 4 1.1484956243825326 2.8542223024892044 5 0.6169808391280108 1.9166382024686006 6 0.3982533956269523 1.484599113451083 7 0.2659117233009849 1.1564689680096503 8 0.20597973695755797 1.0237948610404184 9 0.15272301876102737 0.8539756123080621 >10 0.8277717380886127 9.369206143937827 >50 0.05504259744334017 2.309082843491128 >100 0.025781295811444623 2.975440864391047 >500 0.002026436409611823 0.9011760683583808 >1k 0.002026436409611823 3.0157643094799713 >5k 3.3773940160197047E-4 1.2135520634909893 >10k+ 1.1257980053399015E-4 0.9076110418362581 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12976 0.9039598401072269 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6782 0.4724611309808271 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5297 0.3690101166035743 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5271 0.3671988530521881 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4654 0.3242161756981376 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4394 0.30610354018427516 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4390 0.3058248842532927 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4323 0.3011573974093358 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2906 0.20244353385878555 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2675 0.18635115384454623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2605 0.18147467505235249 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2447 0.17046776577854378 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2286 0.1592518645564982 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2192 0.15270345017840944 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1821 0.1268581125797827 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3932796549124952E-4 0.0 10-11 0.0 0.0 0.0 2.0899194823687428E-4 0.0 12-13 0.0 0.0 0.0 3.1348792235531137E-4 0.0 14-15 0.0 0.0 0.0 6.966398274562475E-4 0.0 16-17 0.0 0.0 0.0 0.0012539516894212455 0.0 18-19 0.0 0.0 0.0 0.0017764315600134312 0.0 20-21 0.0 0.0 0.0 0.002891055283943427 0.0 22-23 0.0 0.0 0.0 0.004179838964737485 0.0 24-25 0.0 0.0 0.0 0.005398958662785919 0.0 26-27 0.0 0.0 0.0 0.006652910352207164 0.0 28-29 0.0 0.0 0.0 0.010867581308317463 0.0 30-31 0.0 0.0 0.0 0.016684523867577128 0.0 32-33 0.0 0.0 0.0 0.027029625305302403 0.0 34-35 0.0 0.0 0.0 0.03678258288968987 0.0 36-37 0.0 0.0 0.0 0.04468944493131828 0.0 38-39 0.0 0.0 0.0 0.052526642990201065 0.0 40-41 0.0 0.0 0.0 0.0658672956859882 0.0 42-43 0.0 0.0 0.0 0.09146880934500531 0.0 44-45 0.0 0.0 0.0 0.12563899288173425 0.0 46-47 0.0 0.0 0.0 0.16496431114163942 0.0 48-49 0.0 0.0 0.0 0.24155986017045383 0.0 50-51 0.0 0.0 0.0 0.3014012213489455 0.0 52-53 0.0 0.0 0.0 0.3645516217078544 0.0 54-55 0.0 0.0 0.0 0.5055515227849988 0.0 56-57 0.0 0.0 0.0 0.653657150102197 0.0 58-59 0.0 0.0 0.0 0.8314744660604043 0.0 60-61 0.0 0.0 0.0 1.01138170150098 0.0 62-63 0.0 0.0 0.0 1.3153256582201411 0.0 64-65 0.0 0.0 0.0 1.4880923354292903 0.0 66-67 0.0 0.0 0.0 1.8320582502358125 0.0 68-69 0.0 0.0 0.0 2.2567298890531413 0.0 70-71 0.0 0.0 0.0 2.3995758856730447 0.0 72-73 0.0 0.0 0.0 2.6804958960947767 0.0 74-75 0.0 0.0 0.0 3.0909909144233705 0.0 76-77 0.0 0.0 0.0 3.2335234231209187 0.0 78-79 0.0 0.0 0.0 3.3666861261391805 0.0 80-81 0.0 0.0 0.0 3.6711177307375604 0.0 82-83 0.0 0.0 0.0 4.12912358529867 0.0 84-85 0.0 0.0 0.0 4.817438566816816 0.0 86-87 0.0 0.0 0.0 5.880545775506422 0.0 88-89 0.0 0.0 0.0 6.77287173049513 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 270 0.0 52.772144 2 TAACGCA 300 0.0 47.48501 4 ATAACGC 315 0.0 46.741047 3 TACCTGG 1725 0.0 44.603935 2 ACCTGGG 1870 0.0 40.637375 3 GTACCTG 2205 0.0 35.785744 1 GTACCCG 190 0.0 35.02555 1 TATCACG 240 0.0 31.663286 2 GTACACG 190 1.2732926E-11 30.0219 1 CCTGGGG 1410 0.0 28.962484 4 TACCCGG 215 1.8189894E-12 28.717867 2 GTATAAC 560 0.0 27.162674 1 GGACGGT 90 8.9651486E-4 26.380558 6 TAACCGT 200 7.221388E-10 26.116753 7 TACCGCG 250 1.4551915E-11 24.692204 8 GTACCGG 100 0.0016468347 23.76734 1 TCACGCA 320 0.0 23.742502 4 ACACGGG 230 3.6325218E-9 22.714968 3 TACACGG 230 3.6325218E-9 22.714968 2 AACCGTG 235 4.669346E-9 22.227024 8 >>END_MODULE