##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003827_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2641791 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80471316618158 31.0 31.0 34.0 30.0 34.0 2 31.998778858736365 31.0 31.0 34.0 30.0 34.0 3 32.04310106287742 33.0 31.0 34.0 30.0 34.0 4 35.80114096838092 37.0 35.0 37.0 35.0 37.0 5 35.65501131618663 37.0 35.0 37.0 33.0 37.0 6 35.71282588213829 37.0 35.0 37.0 33.0 37.0 7 35.66654477965895 37.0 35.0 37.0 33.0 37.0 8 35.6615833727952 37.0 35.0 37.0 33.0 37.0 9 37.2686385864741 39.0 37.0 39.0 33.0 39.0 10-11 37.18619622066999 39.0 37.0 39.0 33.0 39.0 12-13 37.151962437603885 39.0 37.0 39.0 33.0 39.0 14-15 38.329679183553885 40.0 38.0 41.0 33.0 41.0 16-17 38.327038361475225 40.0 38.0 41.0 33.0 41.0 18-19 38.34475418380939 40.0 38.0 41.0 33.5 41.0 20-21 38.297234527636746 40.0 38.0 41.0 33.5 41.0 22-23 38.21078219283812 40.0 38.0 41.0 33.0 41.0 24-25 38.109440905809734 40.0 37.0 41.0 33.0 41.0 26-27 37.94024849051269 40.0 37.0 41.0 33.0 41.0 28-29 37.788050985108214 40.0 37.0 41.0 33.0 41.0 30-31 37.58959035745069 39.5 37.0 41.0 32.0 41.0 32-33 37.33321258191886 39.0 36.0 41.0 31.5 41.0 34-35 37.2948141999121 39.0 36.0 41.0 31.5 41.0 36-37 37.15513093200787 39.0 36.0 41.0 31.0 41.0 38-39 36.93076534063444 39.0 36.0 41.0 31.0 41.0 40-41 36.94014893683868 39.0 36.0 41.0 31.0 41.0 42-43 36.815682239813825 39.0 36.0 41.0 30.0 41.0 44-45 36.578691879864834 39.0 35.0 41.0 30.0 41.0 46-47 36.396651930451725 39.0 35.0 41.0 30.0 41.0 48-49 36.1544357596797 39.0 35.0 41.0 29.5 41.0 50-51 35.94050286339835 39.0 35.0 41.0 28.5 41.0 52-53 35.75120666244983 39.0 35.0 40.5 28.0 41.0 54-55 35.56358697565402 39.0 35.0 40.0 28.0 41.0 56-57 35.30728679899356 38.0 34.5 40.0 27.0 41.0 58-59 35.04705595559982 38.0 34.0 40.0 26.5 41.0 60-61 34.71392740758069 38.0 34.0 40.0 26.0 41.0 62-63 34.463777793171374 37.5 34.0 40.0 26.0 41.0 64-65 34.0244303958943 37.0 33.0 39.5 25.5 41.0 66-67 33.67749549453382 36.5 33.0 39.0 24.5 41.0 68-69 33.367620489281705 36.0 33.0 39.0 24.0 40.5 70-71 32.944329244819144 35.5 32.0 39.0 23.0 40.0 72-73 32.552329461338914 35.0 32.0 38.0 22.5 40.0 74-75 32.16512377398515 35.0 31.5 37.0 22.0 39.5 76-77 31.14365216627659 34.0 30.5 36.0 20.5 39.0 78-79 31.571342320418232 35.0 31.0 36.0 21.0 39.0 80-81 31.37328842440602 35.0 31.0 36.0 20.0 38.5 82-83 31.066806950284864 35.0 31.0 35.5 20.0 37.0 84-85 30.72787400668713 34.0 31.0 35.0 18.5 37.0 86-87 30.4474578041942 34.0 31.0 35.0 18.0 36.5 88-89 30.141605448727773 34.0 31.0 35.0 14.0 36.0 90-91 29.85695348345119 34.0 30.0 35.0 8.5 36.0 92-93 29.61495875336088 34.0 30.0 35.0 4.5 35.5 94-95 29.286388665870994 34.0 30.0 35.0 2.0 35.0 96-97 28.9680413779894 34.0 29.5 35.0 2.0 35.0 98-99 28.62839130726087 34.0 29.0 35.0 2.0 35.0 100-101 27.747169249952023 33.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.006201019335470903 1101 2 0.11264357568958516 1101 3 0.12104653198808535 1101 4 0.057120197928945515 1101 5 0.08248444813855826 1101 6 0.051867234062513035 1101 7 0.0032365125975601927 1101 8 -0.013507111750136858 1101 9 0.08333779926969243 1101 10-11 0.1180561652921952 1101 12-13 0.13069860732793614 1101 14-15 0.21723369645656732 1101 16-17 0.15317791393396618 1101 18-19 0.11212779625192582 1101 20-21 -0.002245704252246128 1101 22-23 -0.058202251422024176 1101 24-25 0.06477459683371478 1101 26-27 0.156783091241671 1101 28-29 0.11252098416520795 1101 30-31 0.15630773315698576 1101 32-33 0.2645517034262852 1101 34-35 0.10311995868490698 1101 36-37 0.1872238437143423 1101 38-39 0.15297215252842022 1101 40-41 0.19201758275311676 1101 42-43 0.10414193874283484 1101 44-45 0.28257471757559216 1101 46-47 0.2525140163526771 1101 48-49 0.3226722223755516 1101 50-51 0.2946308861952218 1101 52-53 0.22711846892149623 1101 54-55 0.21805799085394284 1101 56-57 0.1397795714980532 1101 58-59 0.07511864647742783 1101 60-61 -1.3086573981269112E-4 1101 62-63 -0.0061528340317877905 1101 64-65 -0.014623301224794716 1101 66-67 0.012693248281230751 1101 68-69 0.07167312313959684 1101 70-71 -0.019464527276781496 1101 72-73 0.05066721593544088 1101 74-75 0.13095399729017032 1101 76-77 0.02090041636378359 1101 78-79 0.08145492504073815 1101 80-81 -0.00511849041953738 1101 82-83 0.12217723594773489 1101 84-85 0.1879470656231632 1101 86-87 0.02618074985091212 1101 88-89 0.13598555530177237 1101 90-91 0.09930888913894975 1101 92-93 0.2628360108616654 1101 94-95 0.008804118014371198 1101 96-97 -0.12119340550195545 1101 98-99 -0.20969874505958686 1101 100-101 -0.11279245576714558 1102 1 -0.030804987114454008 1102 2 0.08406718054636642 1102 3 0.14573511479411394 1102 4 0.06116331939303166 1102 5 0.03536997792788554 1102 6 -0.037492892334661576 1102 7 -0.003748161308955389 1102 8 -0.025408172936366213 1102 9 0.07366093909487148 1102 10-11 0.09889285901629563 1102 12-13 0.04227620669188781 1102 14-15 0.07056062858442402 1102 16-17 0.10045452057843818 1102 18-19 -0.02820247350901184 1102 20-21 -0.032552712749094326 1102 22-23 -0.004944718142539273 1102 24-25 -0.0237402266537714 1102 26-27 -0.060640970705726716 1102 28-29 -0.05185756627647464 1102 30-31 0.03649406537277855 1102 32-33 0.023765965137776845 1102 34-35 0.023387787640054114 1102 36-37 0.034447296627227786 1102 38-39 0.058458438357249065 1102 40-41 0.040350775666667005 1102 42-43 0.243259393116098 1102 44-45 0.283816827436155 1102 46-47 0.054619381066444816 1102 48-49 0.14641058690144604 1102 50-51 0.29575787604044734 1102 52-53 0.2494642807318641 1102 54-55 0.1811974335132831 1102 56-57 0.40495659000828965 1102 58-59 0.3044799034959951 1102 60-61 0.19881643374557711 1102 62-63 0.02808356800652234 1102 64-65 -0.17523618033401078 1102 66-67 -0.002680418457096323 1102 68-69 0.1470446545287487 1102 70-71 -0.009147304450920046 1102 72-73 0.17187448958358686 1102 74-75 0.10145585518922218 1102 76-77 0.037511714055671774 1102 78-79 0.2219809351432147 1102 80-81 0.37013444677504737 1102 82-83 0.18130244004062845 1102 84-85 0.17875429056016756 1102 86-87 0.12105247395411212 1102 88-89 0.028030217509869004 1102 90-91 0.0513980208410878 1102 92-93 -0.021138375116500185 1102 94-95 -0.012188821775563241 1102 96-97 0.11669702203271726 1102 98-99 0.16275578313961248 1102 100-101 0.3393678542410079 1103 1 0.01614833224777712 1103 2 0.06070541479517644 1103 3 0.04590990675950479 1103 4 0.06360213454554753 1103 5 0.06599150813120502 1103 6 0.07991261889736734 1103 7 0.03906284889975353 1103 8 0.00156126221443742 1103 9 0.13359880445757 1103 10-11 0.06637251513111408 1103 12-13 0.10023521560152204 1103 14-15 0.07912558808047976 1103 16-17 0.07882458933096359 1103 18-19 0.02662222899230926 1103 20-21 0.11896672117617157 1103 22-23 0.11849004355213566 1103 24-25 0.08823692936911698 1103 26-27 -0.11012808420255027 1103 28-29 -0.08120760365530799 1103 30-31 -0.04220214894882446 1103 32-33 0.10346673538668227 1103 34-35 0.08740591943411147 1103 36-37 -0.011796361227844443 1103 38-39 0.11576791358305627 1103 40-41 0.12461474983238219 1103 42-43 0.34557818240981675 1103 44-45 0.3096074508659399 1103 46-47 0.11227507000108972 1103 48-49 0.17919633576475746 1103 50-51 0.17799159786845564 1103 52-53 0.23672369284260952 1103 54-55 0.24059529740533492 1103 56-57 0.07216517304443215 1103 58-59 0.027888469374651947 1103 60-61 0.051148244351360006 1103 62-63 0.07599203890619322 1103 64-65 0.04138789898262729 1103 66-67 -0.07922658437006191 1103 68-69 -0.04350156653330117 1103 70-71 -0.08043822200916395 1103 72-73 0.06139669934619718 1103 74-75 -0.16039621964196726 1103 76-77 -0.07401888755958552 1103 78-79 0.02258213678053167 1103 80-81 0.12931715263433574 1103 82-83 0.20048666249978808 1103 84-85 -0.03628042272586285 1103 86-87 -0.0196374411162914 1103 88-89 -0.012981553756233666 1103 90-91 0.11976220702440443 1103 92-93 0.06846089017511403 1103 94-95 0.035237477598190736 1103 96-97 0.03472747964757872 1103 98-99 -0.02948320848998165 1103 100-101 0.05273737150267266 1104 1 0.030411021031934382 1104 2 -0.026833829777711316 1104 3 0.09447857406523497 1104 4 -0.01148247828451332 1104 5 -0.021187773717642244 1104 6 -0.06111504523896372 1104 7 -0.05682698130932806 1104 8 -0.06325008512957453 1104 9 -0.0675865995053968 1104 10-11 -0.09823113725402521 1104 12-13 -0.0873550204194018 1104 14-15 -0.03682617769958796 1104 16-17 0.006122717760320029 1104 18-19 -0.04448355922399827 1104 20-21 -0.12400693991558853 1104 22-23 -0.08168652267826104 1104 24-25 0.059103893898068804 1104 26-27 -0.040673728025517164 1104 28-29 -0.06618966047524566 1104 30-31 -0.12290112040539469 1104 32-33 -0.00973840097127976 1104 34-35 0.05140748803939488 1104 36-37 0.15135860802419643 1104 38-39 0.2397487715740283 1104 40-41 0.23268462735084938 1104 42-43 0.03354526054096851 1104 44-45 0.36156907106719416 1104 46-47 0.3710152554231101 1104 48-49 0.20410913214518445 1104 50-51 0.3304360077690376 1104 52-53 0.3736371047318201 1104 54-55 0.1889339732606672 1104 56-57 0.19611698278940537 1104 58-59 0.23293886023057553 1104 60-61 0.2200325875822955 1104 62-63 0.07290212987430778 1104 64-65 0.12501701411810018 1104 66-67 0.09943661534919102 1104 68-69 0.13866822753033858 1104 70-71 0.11045448554644821 1104 72-73 0.20170247531513752 1104 74-75 0.10648159636958354 1104 76-77 -0.004510360329209817 1104 78-79 0.11310137810315268 1104 80-81 0.06412130857474097 1104 82-83 0.13739367592400598 1104 84-85 0.2317092737827764 1104 86-87 0.36459685889556326 1104 88-89 0.16208103736301283 1104 90-91 0.053079210532960275 1104 92-93 0.19383096090366791 1104 94-95 0.26534492470486626 1104 96-97 0.25762193323829763 1104 98-99 0.2539632952395756 1104 100-101 0.43556832719781724 1105 1 -0.13859404578722945 1105 2 -0.08455100251646286 1105 3 0.0012807853049849882 1105 4 -0.05344294385685089 1105 5 -0.056126940505251355 1105 6 0.03770266041136239 1105 7 -0.06064055996808548 1105 8 -0.02295206467400135 1105 9 -0.16861107736867353 1105 10-11 -0.14446762654707612 1105 12-13 -0.14979353180812893 1105 14-15 -0.06707422339126623 1105 16-17 -0.05829631104886346 1105 18-19 -0.10453536348606463 1105 20-21 -0.15101033291523436 1105 22-23 -0.13993246835271833 1105 24-25 -0.009809533563284845 1105 26-27 -0.08282803817931494 1105 28-29 -0.1690124596677407 1105 30-31 -0.04004479274679085 1105 32-33 -0.10380626343084032 1105 34-35 -0.21676652067065305 1105 36-37 -0.22861480052824135 1105 38-39 -0.27708973773167145 1105 40-41 -0.07642854842089974 1105 42-43 -0.13105571768554114 1105 44-45 -0.015806587123897486 1105 46-47 -0.0023416736401884464 1105 48-49 -0.15391523183696876 1105 50-51 -0.2123923388456319 1105 52-53 -0.16792484543374542 1105 54-55 -0.16051915753146062 1105 56-57 -0.26263776893809876 1105 58-59 -0.11779135621345205 1105 60-61 -0.11880883713494939 1105 62-63 -0.31441277390300115 1105 64-65 -0.4533860957273035 1105 66-67 -0.32530728574115386 1105 68-69 -0.31620223235069034 1105 70-71 -0.2648293778822648 1105 72-73 -0.31445747517467026 1105 74-75 -0.1682940228744343 1105 76-77 -0.269335359578907 1105 78-79 -0.2595606157373993 1105 80-81 -0.28157703417399915 1105 82-83 -0.2089845275307205 1105 84-85 -0.25533363089570926 1105 86-87 -0.2216541814173958 1105 88-89 -0.08918470814104751 1105 90-91 -0.27467675715988094 1105 92-93 -0.09698298488809698 1105 94-95 -0.23121972996604612 1105 96-97 -0.21913192854455588 1105 98-99 -0.1993890237624143 1105 100-101 -0.07647614317479778 1106 1 -0.049632850069134093 1106 2 0.056159336808118354 1106 3 0.027390352684392383 1106 4 -0.019714158472211807 1106 5 -0.027422806345938966 1106 6 0.04039411005434346 1106 7 -0.06733465589726961 1106 8 0.034353784617927374 1106 9 0.01571111919456314 1106 10-11 0.05116385959670566 1106 12-13 -0.024886535714472302 1106 14-15 0.07783281932270825 1106 16-17 0.072710663206081 1106 18-19 -0.006621826997857738 1106 20-21 0.03288878419779451 1106 22-23 0.05318654648987575 1106 24-25 0.07443747315689109 1106 26-27 0.1354405295670844 1106 28-29 0.054048823186654715 1106 30-31 0.09673589023805818 1106 32-33 0.16929625655799185 1106 34-35 0.28043757102044964 1106 36-37 0.16251799907036713 1106 38-39 0.2247677180019636 1106 40-41 0.2303876053469267 1106 42-43 0.1646056491728629 1106 44-45 0.33617956256027526 1106 46-47 0.17302344727617935 1106 48-49 0.256698890421049 1106 50-51 0.25988874558392183 1106 52-53 0.2774731384411595 1106 54-55 0.14470971985549141 1106 56-57 0.11640885706884774 1106 58-59 0.2724128247847162 1106 60-61 0.19329041928542523 1106 62-63 0.11039643605877814 1106 64-65 0.2831129130808847 1106 66-67 0.2074801332979206 1106 68-69 0.11597881651629649 1106 70-71 0.21992140515708059 1106 72-73 0.23576266745730123 1106 74-75 0.23341277636489366 1106 76-77 0.2006629314947972 1106 78-79 0.2425999449535965 1106 80-81 0.3093567112053037 1106 82-83 0.4323157045947923 1106 84-85 0.40017895302269935 1106 86-87 0.38895239417549377 1106 88-89 0.327925956418575 1106 90-91 0.47061061999373877 1106 92-93 0.5202222606560589 1106 94-95 0.5836417807160537 1106 96-97 0.5461012991014726 1106 98-99 0.5638436091830918 1106 100-101 0.7068020640522583 1107 1 -0.08470728604239852 1107 2 -0.09073638070528034 1107 3 -0.0700000103837013 1107 4 -0.07377921862700987 1107 5 -0.11409006793399357 1107 6 0.005046305665722173 1107 7 -0.04830980336188162 1107 8 0.027866441927010044 1107 9 -0.08404313956140186 1107 10-11 -0.06473327014189323 1107 12-13 -0.07296201595814011 1107 14-15 0.006656332734877424 1107 16-17 -0.0083566716061938 1107 18-19 0.008367976314403336 1107 20-21 -0.09302479959947618 1107 22-23 -0.005455868369381278 1107 24-25 -0.12888712379660205 1107 26-27 -0.021129969687073924 1107 28-29 0.03927890648685661 1107 30-31 0.03816957234405294 1107 32-33 -0.008576732411619048 1107 34-35 -0.07733979859779794 1107 36-37 -0.08027655043101589 1107 38-39 0.08633442616032028 1107 40-41 0.1986912328495265 1107 42-43 0.07956721409319556 1107 44-45 0.15688693733416414 1107 46-47 0.13775624760477 1107 48-49 0.1333062096044202 1107 50-51 0.03357384961825716 1107 52-53 0.08115786657482005 1107 54-55 0.10985937263634327 1107 56-57 0.19584201719244732 1107 58-59 0.19229792447938365 1107 60-61 -0.0934631924935232 1107 62-63 0.08111782306502135 1107 64-65 -0.1069207071084648 1107 66-67 0.005207141394457437 1107 68-69 -0.03931637094658669 1107 70-71 -0.02407541527333734 1107 72-73 0.15100287257071443 1107 74-75 0.15924696260564986 1107 76-77 0.13261482476590203 1107 78-79 0.22121287758699992 1107 80-81 0.29700883255338084 1107 82-83 0.23770818309739994 1107 84-85 0.1742665458250876 1107 86-87 0.22626724399400544 1107 88-89 0.0443508445881271 1107 90-91 0.05988802277069638 1107 92-93 0.07336972587836854 1107 94-95 0.1335490127088086 1107 96-97 0.09140328944968701 1107 98-99 0.12260139193145037 1107 100-101 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-0.8871776823018429 2316 98-99 -0.8087337875098513 2316 100-101 -1.1157729338269355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 18.0 5 43.0 6 109.0 7 281.0 8 758.0 9 1469.0 10 2454.0 11 4231.0 12 6586.0 13 9540.0 14 10700.0 15 13886.0 16 17579.0 17 23156.0 18 20371.0 19 14057.0 20 12288.0 21 12536.0 22 13276.0 23 14607.0 24 17074.0 25 20786.0 26 26194.0 27 33681.0 28 41862.0 29 52890.0 30 66256.0 31 82642.0 32 102941.0 33 131615.0 34 173705.0 35 235075.0 36 346484.0 37 512145.0 38 509077.0 39 111351.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07043816492636 20.228039676858575 14.487166376930155 21.214355781284915 2 15.269005011170844 22.04052090738608 39.338675351989515 23.351798729453556 3 20.7643504577484 26.474807176137706 30.15072979211982 22.610112573994073 4 12.680261231868833 17.18023870926958 39.64613400530171 30.49336605355988 5 12.241884388280527 38.5148560200258 35.00182262714954 14.24143696454413 6 28.47969426801742 38.5008882231789 18.06857544748998 14.950842061313708 7 26.73084282594649 33.18048248328501 22.55704558006292 17.53162911070558 8 25.00954844648952 33.33000225983055 20.347029723395984 21.313419570283948 9 24.256990806615665 19.052604842699516 23.099442764397335 33.590961586287484 10-11 24.422049283989537 26.357270503230573 28.28194963189745 20.93873058088244 12-13 26.636796779154746 24.233181201692336 28.07129708595419 21.05872493319873 14-15 22.277484453475502 26.354368395471482 28.293160437710185 23.07498671334283 16-17 20.386340176039663 29.473338352655453 28.69731178583014 21.44300968547474 18-19 20.12698960667214 29.89231547840083 30.108873109189936 19.871821805737092 20-21 21.58957355319214 27.334878280955216 29.386353694576993 21.689194471275652 22-23 20.67404272328886 27.569913744122832 29.864569150246933 21.891474382341375 24-25 22.061340204429495 27.53813605996841 29.718702198622072 20.681821536980028 26-27 21.594382489703005 26.506524191055767 31.42409806529472 20.474995253946513 28-29 20.47307751569095 28.897524163436483 30.489617416830118 20.13978090404245 30-31 21.225415197277503 27.601114574782077 29.623226251450145 21.550243976490275 32-33 21.245874048681827 27.716683067317245 30.155468325167078 20.881974558833857 34-35 22.403065796053472 26.750043438546093 30.298811771221384 20.548078994179043 36-37 22.09818838323156 26.663832160376515 30.890054536481554 20.34792491991037 38-39 21.302577351015298 27.833130164197772 30.864705260849856 19.999587223937073 40-41 20.923480227699677 28.317549247389167 29.941958349697867 20.81701217521329 42-43 20.48229624497924 28.637740249022347 28.901053556083028 21.97890994991539 44-45 21.42699767901685 27.193794710914375 29.02981692966221 22.349390680406565 46-47 22.35314069315034 28.22760760395476 28.83275327273044 20.58649843016446 48-49 22.159101555380296 27.905107398820977 29.155161576604737 20.780629469193993 50-51 20.55022964877658 28.254720400461586 30.22994403523571 20.96510591552612 52-53 21.362251017261315 29.438867700479353 28.068217026093635 21.130664256165694 54-55 20.7145243270815 28.294686397218516 28.747699211604395 22.24309006409559 56-57 22.170273555160872 27.486862087456863 28.629188037917142 21.713676319465122 58-59 23.322904554623513 27.405535764073502 28.199987999554054 21.071571681748928 60-61 21.80341403750649 28.05190218286258 29.923668138867658 20.221015640763273 62-63 21.006527772940707 28.29918656468946 29.381129724155784 21.313155938214045 64-65 20.66340628966648 29.727126824018807 28.644524335591807 20.964942550722903 66-67 21.43024425005817 27.804949068738793 28.01029094895931 22.75451573224373 68-69 21.013683381544475 28.63241908858229 28.230020142664586 22.12387738720865 70-71 22.414296363404393 28.146220998796473 28.093786663809595 21.345695973989542 72-73 21.9584236130984 27.15287903495663 29.6729147077661 21.215782644178866 74-75 20.88649269441852 29.722594393215335 28.522268022544633 20.868644889821507 76-77 21.4626056795601 28.149179442658077 27.986201683226575 22.402013194555245 78-79 21.507138763573266 28.368115516315928 28.59951305448536 21.525232665625445 80-81 22.35075792131397 28.075794024071026 28.15823890467169 21.415209149943315 82-83 21.82663930369398 27.69781769378441 29.33113581635339 21.14440718616821 84-85 21.11340629207648 28.440017609425812 28.891457876489486 21.555118222008225 86-87 21.316673278600653 28.757900251657276 28.03294711563213 21.89247935410995 88-89 20.970804989198598 28.59808531119277 27.667238123285486 22.763871576323147 90-91 21.49116074749197 26.87810339100621 28.32900700004826 23.30172886145355 92-93 21.878442874752746 28.032838483620033 28.542192314760683 21.54652632686654 94-95 21.311367122379174 27.642989956558672 29.13758212440058 21.908060796661573 96-97 19.690698390141655 28.167959477010868 30.340456250820978 21.800885882026495 98-99 20.67591099401125 29.12860290276421 28.035126172575918 22.160359930648617 100-101 19.91195852810195 28.168652587299498 28.370727393590844 23.548661491007707 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 370.0 1 297.0 2 556.5 3 840.0 4 630.5 5 486.5 6 655.0 7 1096.0 8 1656.5 9 2046.0 10 2085.0 11 2035.0 12 2163.0 13 2394.5 14 2671.0 15 2942.0 16 3208.5 17 3603.0 18 3954.0 19 4205.5 20 4528.0 21 4943.5 22 5596.5 23 6696.0 24 8137.0 25 9958.0 26 12533.0 27 16146.0 28 21327.5 29 27702.5 30 34211.0 31 40304.0 32 46489.5 33 52398.5 34 58535.0 35 65323.5 36 71295.0 37 78041.5 38 84657.5 39 88422.5 40 91288.0 41 94092.5 42 97420.0 43 102382.0 44 103890.0 45 104940.0 46 110199.5 47 135614.5 48 191950.5 49 185806.5 50 127320.5 51 97525.0 52 78129.0 53 65200.5 54 56408.5 55 48667.5 56 40707.0 57 32575.5 58 25912.5 59 21007.5 60 16572.5 61 12425.0 62 9120.0 63 6497.0 64 4340.0 65 2763.5 66 1689.0 67 1017.0 68 603.0 69 346.0 70 178.0 71 98.0 72 59.0 73 33.0 74 16.5 75 12.5 76 10.5 77 5.5 78 3.5 79 4.0 80 2.0 81 0.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05530339076785408 2 0.003520339042717611 3 0.0393293791976731 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0016276836434070674 16-17 0.0 18-19 0.0 20-21 0.010504237466173516 22-23 0.0 24-25 0.0 26-27 0.004637005728310831 28-29 0.011355932395863261 30-31 0.009292938010614768 32-33 0.004826271268241885 34-35 0.004845197822234991 36-37 0.020118926894671076 38-39 0.04292542445636313 40-41 0.005810452075883368 42-43 0.009879661184401037 44-45 0.009009039700718188 46-47 0.004277401202441828 48-49 9.652542536483772E-4 50-51 0.0014951977654553295 52-53 6.245762817724794E-4 54-55 0.001268079117538064 56-57 0.011147740301939102 58-59 0.008422316526931917 60-61 0.006359322141683426 62-63 0.005867231737862685 64-65 0.06851412545504168 66-67 0.03139915307456192 68-69 0.023885311139299058 70-71 0.014838418330594662 72-73 1.1355932395863261E-4 74-75 3.5960452586900326E-4 76-77 7.38135605731112E-4 78-79 6.435028357655848E-4 80-81 9.841808076414826E-4 82-83 8.138418217035337E-4 84-85 9.084745916690609E-4 86-87 0.0038988701225797194 88-89 1.5141243194484347E-4 90-91 8.895480376759553E-4 92-93 4.731638498276359E-4 94-95 0.002536158235076128 96-97 0.004920904038207413 98-99 0.006472881465642059 100-101 0.00738135605731112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2641791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.313013502272895 #Duplication Level Percentage of deduplicated Percentage of total 1 77.72258917169665 40.65902856770576 2 13.629633459881296 14.260143984356013 3 3.6850353921280457 5.783259186742439 4 1.5229920756591873 3.1868922007125473 5 0.8119993474107803 2.123906641246846 6 0.5094434285733794 1.5990312574562036 7 0.353651934558834 1.2950418899376848 8 0.24606535300564994 1.0297936107380887 9 0.20341983798142044 0.957735425785699 >10 1.1630463022979105 11.479244325406347 >50 0.09529080352613419 3.4055103549697234 >100 0.050301178785838535 4.558834246440994 >500 0.003411006458458367 1.180589590857543 >1k 0.0021046635594716917 2.3437528046159515 >5k 6.531714494912147E-4 2.5559066068318477 >10k+ 3.6287302749511924E-4 3.581329306196405 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 39674 1.50178420624493 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15881 0.6011452079290148 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14026 0.5309276926145937 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13680 0.5178305172513646 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11069 0.4189960522993681 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8825 0.3340536779783109 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8560 0.32402260436196506 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8162 0.30895706738345313 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8153 0.3086163894115772 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7116 0.2693627164298766 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6990 0.26459322482361397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6684 0.2530101737798334 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6650 0.2517231681083023 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6181 0.23397006046276939 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4002 0.15148813816081588 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3677 0.13918587806529736 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3568 0.1350598892948004 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3405 0.12888983269304802 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3356 0.12703503040172368 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3170 0.11999435231628845 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3137 0.1187451997527435 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3038 0.11499774206210862 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2999 0.1135214708506464 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2816 0.1065943520891698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1355932395863261E-4 0.0 12-13 0.0 0.0 0.0 2.838983098965815E-4 0.0 14-15 0.0 0.0 0.0 4.3531074184142505E-4 0.0 16-17 0.0 0.0 0.0 7.570621597242174E-4 0.0 18-19 0.0 0.0 0.0 0.001003107361634588 0.0 20-21 0.0 0.0 0.0 0.0014573446574691184 0.0 22-23 0.0 0.0 0.0 0.001911581953303649 0.0 24-25 0.0 0.0 0.0 0.002649717559034761 0.0 26-27 0.0 0.0 0.0 0.003633898366676243 0.0 28-29 0.0 0.0 0.0 0.0052237289020971005 0.0 30-31 0.0 0.0 0.0 0.007854519907138756 0.0 32-33 0.0 0.0 0.0 0.012718644283366852 0.0 34-35 0.0 0.0 0.0 0.017412429673657 0.0 36-37 0.0 0.0 0.0 0.021462712228181562 0.0 38-39 0.0 0.0 0.0 0.024774859176975014 0.0 40-41 0.0 0.0 0.0 0.03054745814487217 0.0 42-43 0.0 0.0 0.0 0.038931921563817876 0.0 44-45 0.0 0.0 0.0 0.05269152631680553 0.0 46-47 0.0 0.0 0.0 0.08554802404883657 0.0 48-49 0.0 0.0 0.0 0.186331924062123 0.0 50-51 0.0 0.0 0.0 0.23022260277213452 0.0 52-53 0.0 0.0 0.0 0.26762147346251086 0.0 54-55 0.0 0.0 0.0 0.32544209591144796 0.0 56-57 0.0 0.0 0.0 0.35638701169017534 0.0 58-59 0.0 0.0 0.0 0.40046695594011794 0.0 60-61 0.0 0.0 0.0 0.514480517194585 0.0 62-63 0.0 0.0 0.0 0.5694432299905632 0.0 64-65 0.0 0.0 0.0 0.6240841913686586 0.0 66-67 0.0 0.0 0.0 0.8158101833188167 0.0 68-69 0.0 0.0 0.0 0.998432502798291 0.0 70-71 0.0 1.8926553993105437E-5 0.0 1.1000870242952603 0.0 72-73 0.0 3.785310798621087E-5 0.0 1.1990918282331948 0.0 74-75 0.0 3.785310798621087E-5 0.0 1.2591647106073114 0.0 76-77 0.0 3.785310798621087E-5 0.0 1.2921915473252805 0.0 78-79 0.0 3.785310798621087E-5 0.0 1.3543463506386386 0.0 80-81 0.0 3.785310798621087E-5 0.0 1.4890655619615631 0.0 82-83 0.0 3.785310798621087E-5 0.0 1.6604265818151398 0.0 84-85 0.0 3.785310798621087E-5 0.0 1.9163514449099117 0.0 86-87 0.0 3.785310798621087E-5 0.0 2.5874870495054303 0.0 88-89 0.0 3.785310798621087E-5 0.0 3.3658415824718912 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 545 0.0 52.298004 2 TACCTGG 3205 0.0 48.912098 2 TAACGCA 615 0.0 46.327847 4 ACCTGGG 3420 0.0 44.86406 3 GTACCTG 3795 0.0 42.32688 1 ATAACGC 830 0.0 34.339607 3 GTATAAC 1040 0.0 33.358067 1 GTACACG 375 0.0 30.415245 1 GTACCCG 365 0.0 29.946518 1 GTACCGG 210 1.8189894E-12 29.419506 1 CCTGGGG 2645 0.0 27.827358 4 TACACGG 415 0.0 27.472204 2 CCGCGTA 120 2.3101165E-10 23.743471 10-11 TATCACG 445 0.0 23.485136 2 TCACGCA 445 0.0 23.476244 4 TACCCGG 450 0.0 23.22419 2 TACGCAG 675 0.0 21.104906 4 AGTACTT 10060 0.0 20.368404 12-13 ACACGGG 540 0.0 20.232811 3 GAGTACG 885 0.0 19.868715 1 >>END_MODULE