##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003826_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3514055 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.960720592022607 31.0 31.0 34.0 28.0 34.0 2 30.673740166275145 31.0 31.0 34.0 27.0 34.0 3 30.847967376720057 31.0 31.0 34.0 27.0 34.0 4 34.22367378996629 37.0 35.0 37.0 30.0 37.0 5 34.17581824985665 37.0 35.0 37.0 30.0 37.0 6 34.31428278726428 37.0 35.0 37.0 30.0 37.0 7 34.27656169297293 37.0 35.0 37.0 30.0 37.0 8 34.34495589852749 37.0 35.0 37.0 30.0 37.0 9 35.80397859452968 39.0 35.0 39.0 30.0 39.0 10-11 35.709883880588094 39.0 35.0 39.0 30.0 39.0 12-13 35.7307341518559 39.0 35.0 39.0 30.0 39.0 14-15 36.653117694515316 39.0 36.0 41.0 30.0 41.0 16-17 36.68355731483997 39.5 36.0 41.0 30.0 41.0 18-19 36.62142169089556 40.0 36.0 41.0 30.0 41.0 20-21 36.53334694533808 39.5 36.0 41.0 29.5 41.0 22-23 36.349769852776916 39.0 36.0 41.0 29.0 41.0 24-25 36.2192835627217 39.0 36.0 41.0 28.0 41.0 26-27 36.062840792190215 39.0 36.0 41.0 28.0 41.0 28-29 35.94881582673009 39.0 36.0 40.0 27.0 41.0 30-31 35.7092925409534 39.0 35.0 40.0 27.0 41.0 32-33 35.44966527274047 38.0 35.0 40.0 26.5 41.0 34-35 35.31875098710749 38.0 35.0 40.0 25.5 41.0 36-37 35.29394759046173 38.0 35.0 40.0 25.5 41.0 38-39 35.237016922045896 38.5 35.0 40.0 25.0 41.0 40-41 35.46823740664276 39.0 35.0 40.5 26.0 41.0 42-43 35.33198342086279 39.0 35.0 41.0 25.0 41.0 44-45 35.106890757259066 39.0 35.0 40.0 24.5 41.0 46-47 34.8520654912914 38.5 34.5 40.0 24.0 41.0 48-49 34.524147886131544 38.0 34.0 40.0 23.0 41.0 50-51 33.66142163398125 37.5 33.0 39.5 21.5 40.5 52-53 33.85441320639546 38.0 33.5 39.5 22.0 40.5 54-55 34.238455573404515 38.0 34.0 40.0 22.0 41.0 56-57 33.85565849140096 38.0 33.5 40.0 19.0 41.0 58-59 33.60646005825179 38.0 33.0 40.0 18.5 41.0 60-61 33.02518344192109 37.5 32.0 40.0 13.0 41.0 62-63 32.98746661620265 37.0 32.0 40.0 13.5 41.0 64-65 32.68129995119598 36.5 32.0 39.5 13.0 41.0 66-67 32.12337527443367 36.0 31.0 39.0 8.0 41.0 68-69 31.736119952590386 36.0 31.0 39.0 8.0 40.0 70-71 31.189236081962292 35.0 30.5 38.5 7.0 40.0 72-73 30.741612467647776 35.0 29.5 37.0 4.0 40.0 74-75 30.353081980788573 35.0 29.0 37.0 2.0 39.0 76-77 29.95822660715327 34.0 29.0 36.5 2.0 39.0 78-79 29.33107777197568 34.0 29.0 36.0 2.0 38.5 80-81 28.785607510411758 34.0 27.5 35.5 2.0 37.0 82-83 28.389501160340405 34.0 26.5 35.0 2.0 37.0 84-85 28.14012984998812 34.0 26.0 35.0 2.0 36.5 86-87 28.12374991284997 34.0 27.0 35.0 2.0 36.0 88-89 27.692337911614928 34.0 26.0 35.0 2.0 36.0 90-91 27.319140138671706 33.0 25.0 35.0 2.0 35.0 92-93 27.12505979559227 33.0 25.0 35.0 2.0 35.0 94-95 26.78562060070204 33.0 25.0 35.0 2.0 35.0 96-97 26.495622151616864 33.0 25.0 35.0 2.0 35.0 98-99 26.305881524335845 33.0 24.5 35.0 2.0 35.0 100-101 25.96044398849762 32.5 23.5 35.0 2.0 35.0 102-103 25.534823871567177 32.0 19.5 35.0 2.0 35.0 104-105 25.13959955094613 32.0 19.0 35.0 2.0 35.0 106-107 24.572423596101938 31.5 17.0 34.0 2.0 35.0 108-109 24.41486274403787 31.0 15.5 34.0 2.0 35.0 110-111 24.152945244169487 31.5 8.5 34.0 2.0 35.0 112-113 23.46179641468332 31.0 2.0 34.0 2.0 35.0 114-115 22.944399276619176 31.0 2.0 34.0 2.0 35.0 116-117 22.295674939635262 30.5 2.0 34.0 2.0 35.0 118-119 21.516965158485 30.0 2.0 34.0 2.0 35.0 120-121 19.954978650021133 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5537590922398614 1101 2 0.5797037125422371 1101 3 0.6323322969827601 1101 4 0.8499249940431071 1101 5 0.9538624444517012 1101 6 0.899120562348692 1101 7 0.8553856300994127 1101 8 0.8352794825766381 1101 9 0.9534966736581154 1101 10-11 0.8771576312780667 1101 12-13 0.6982334101952787 1101 14-15 0.8328633646459664 1101 16-17 0.8508688300805076 1101 18-19 0.9470335041893776 1101 20-21 0.9889098798456502 1101 22-23 1.0519131790629004 1101 24-25 1.0238724096684493 1101 26-27 0.8413451262606131 1101 28-29 0.900243397763262 1101 30-31 1.0013469770202263 1101 32-33 1.0318226834117468 1101 34-35 1.0743850199314195 1101 36-37 0.8829158320540742 1101 38-39 0.8203794379116829 1101 40-41 1.0557573843556014 1101 42-43 1.1061641339630555 1101 44-45 1.0118991469756295 1101 46-47 0.9283176916119587 1101 48-49 1.1224478081725877 1101 50-51 0.8523637298899658 1101 52-53 0.9220206779621734 1101 54-55 0.988813071158269 1101 56-57 0.9992872072080985 1101 58-59 0.9399587967628662 1101 60-61 1.0534487683526805 1101 62-63 0.8929381444013416 1101 64-65 0.8958889814656814 1101 66-67 0.9140058177653714 1101 68-69 0.8020571534404972 1101 70-71 0.6467497486409997 1101 72-73 0.4900082462755364 1101 74-75 0.7384291546058073 1101 76-77 0.6807842718384549 1101 78-79 0.8056722724595744 1101 80-81 0.7638458486672768 1101 82-83 0.8164526371484548 1101 84-85 0.6836789823213252 1101 86-87 0.7711158178611015 1101 88-89 0.778398770115782 1101 90-91 0.6777284288125678 1101 92-93 0.5610499269933236 1101 94-95 0.63466342096293 1101 96-97 0.6037345716142539 1101 98-99 0.4911512496893131 1101 100-101 0.6621035299411702 1101 102-103 0.6312468359357055 1101 104-105 0.5147621093161625 1101 106-107 0.573421174728896 1101 108-109 0.44634668445154446 1101 110-111 0.4810249796671293 1101 112-113 0.4996353481541824 1101 114-115 0.41089736195713655 1101 116-117 0.40679107667092396 1101 118-119 0.2992856254847851 1101 120-121 0.3763830349331698 1102 1 0.5434010486981968 1102 2 0.6702665974776458 1102 3 0.7646145457775582 1102 4 0.9757916241105278 1102 5 1.1228541924659083 1102 6 1.0787321528717797 1102 7 0.9809214944992135 1102 8 0.8541866520254189 1102 9 1.0369878984175074 1102 10-11 1.0023532942676567 1102 12-13 0.8963622166596181 1102 14-15 1.0210553048062039 1102 16-17 1.0840593119896198 1102 18-19 1.1985437376811205 1102 20-21 1.1897779681642007 1102 22-23 1.1437632708469394 1102 24-25 1.0535385559396175 1102 26-27 1.195493764940295 1102 28-29 1.0589259965383278 1102 30-31 1.169263050378838 1102 32-33 1.2196099159942193 1102 34-35 1.1023058969408055 1102 36-37 0.9814128040384915 1102 38-39 1.0294353489755181 1102 40-41 1.0706332614507659 1102 42-43 1.1674067354830768 1102 44-45 1.2271757857925607 1102 46-47 1.2161021792511377 1102 48-49 1.1888735488783837 1102 50-51 0.9564646751798094 1102 52-53 1.0293102180126112 1102 54-55 1.1843834189082756 1102 56-57 1.1936854641691 1102 58-59 1.147548736545673 1102 60-61 1.163578981600459 1102 62-63 1.0046094717529854 1102 64-65 1.0745540812272196 1102 66-67 1.2509307950338098 1102 68-69 1.1210471858651339 1102 70-71 0.9060878741878398 1102 72-73 0.8404421188758562 1102 74-75 0.8399074781316891 1102 76-77 0.9183112494843222 1102 78-79 1.0869117859183746 1102 80-81 1.0787942952009288 1102 82-83 1.074716460511354 1102 84-85 0.9169475235555211 1102 86-87 0.8661670282095315 1102 88-89 0.9927821014475953 1102 90-91 1.0510666229829937 1102 92-93 1.0459795020417886 1102 94-95 1.0599378295134265 1102 96-97 1.0142950547242755 1102 98-99 1.1570316044732358 1102 100-101 1.1498341480680878 1102 102-103 1.081960006516777 1102 104-105 1.1366025937986528 1102 106-107 1.0289410841457673 1102 108-109 1.0191472128531238 1102 110-111 0.9008575381258446 1102 112-113 0.8894562406038773 1102 114-115 0.8154488210660595 1102 116-117 0.7861895909703662 1102 118-119 0.6875020985757629 1102 120-121 0.8013724840573637 1103 1 0.5790342170055354 1103 2 0.9023901140618378 1103 3 0.8176976300884391 1103 4 0.9891308333968496 1103 5 1.0587825695197353 1103 6 1.0651115201720245 1103 7 0.9235081512375416 1103 8 0.8613425786150799 1103 9 0.9545241807990195 1103 10-11 0.9243259573371105 1103 12-13 0.8497855200957858 1103 14-15 0.940677839954283 1103 16-17 1.0129511629763144 1103 18-19 1.0467039022656976 1103 20-21 1.1558856594754943 1103 22-23 1.1626472956721159 1103 24-25 1.153577596292287 1103 26-27 1.0775188484573448 1103 28-29 1.155923037214471 1103 30-31 1.1886398375423397 1103 32-33 1.166580959920232 1103 34-35 1.126964601401717 1103 36-37 1.0573902510888828 1103 38-39 1.0908197727069862 1103 40-41 1.1721037331588064 1103 42-43 1.2040565811488548 1103 44-45 1.0616751556834387 1103 46-47 1.0906571680763335 1103 48-49 1.0554982444522807 1103 50-51 0.8773848769160395 1103 52-53 0.9824449701859663 1103 54-55 1.09752941643994 1103 56-57 1.171042819440295 1103 58-59 1.1822753654311242 1103 60-61 1.1916942588249526 1103 62-63 1.2358284751059756 1103 64-65 1.2598576860013608 1103 66-67 1.0709973553978287 1103 68-69 1.1362293603078548 1103 70-71 0.9900692448117709 1103 72-73 0.880843420413914 1103 74-75 0.9592959158916337 1103 76-77 1.0125917111808143 1103 78-79 0.9436821754571518 1103 80-81 0.9508310546417995 1103 82-83 0.9044293167274375 1103 84-85 1.0862720096719514 1103 86-87 1.1051823429547412 1103 88-89 1.061038893618683 1103 90-91 1.100621747490603 1103 92-93 1.0880003088492103 1103 94-95 0.9412688341747533 1103 96-97 1.0533640273406704 1103 98-99 1.1011172081351397 1103 100-101 1.026916194679881 1103 102-103 0.9791638271887706 1103 104-105 1.0929096369460218 1103 106-107 1.168385372404682 1103 108-109 1.1229366598007609 1103 110-111 0.9766033481625058 1103 112-113 0.8959438137231253 1103 114-115 0.8016869339299468 1103 116-117 0.7718971675124315 1103 118-119 0.6311440026595108 1103 120-121 0.7063429107188455 1104 1 0.5281440956156906 1104 2 0.5593383286906146 1104 3 0.6520074230799047 1104 4 0.85675767206844 1104 5 0.9581024857559655 1104 6 0.8847665371689786 1104 7 0.855987719141865 1104 8 0.684442674125151 1104 9 0.7717666605519611 1104 10-11 0.9246309225128257 1104 12-13 0.7097521361176788 1104 14-15 0.8824133864895671 1104 16-17 0.8952685282416724 1104 18-19 0.8637331348339643 1104 20-21 0.900653793424425 1104 22-23 0.9809592094850714 1104 24-25 0.8484289845919974 1104 26-27 0.7972357493989435 1104 28-29 0.8564430085500234 1104 30-31 0.9648348041777837 1104 32-33 0.9770996999731665 1104 34-35 0.9508725690795217 1104 36-37 0.6001083337419857 1104 38-39 0.6545531541037448 1104 40-41 0.9090954835652525 1104 42-43 0.8518120945651475 1104 44-45 0.763865468116272 1104 46-47 0.8294761967154187 1104 48-49 1.0349181207346447 1104 50-51 0.8010606598712897 1104 52-53 0.8961379979510511 1104 54-55 0.7706233098492419 1104 56-57 0.9968161003333265 1104 58-59 0.879321360804326 1104 60-61 0.9614308168851835 1104 62-63 1.0955558019731342 1104 64-65 1.1331148442878813 1104 66-67 1.2253017389695486 1104 68-69 1.190333494836107 1104 70-71 0.9284630811800199 1104 72-73 0.8353611197018012 1104 74-75 1.0473560381198475 1104 76-77 1.0616893911839433 1104 78-79 1.0458077027784896 1104 80-81 0.9827556544578151 1104 82-83 0.9820167977584866 1104 84-85 1.0594798481221872 1104 86-87 1.125905126411876 1104 88-89 1.1821991593290129 1104 90-91 1.052433937371287 1104 92-93 1.1135722670385988 1104 94-95 1.134290888296821 1104 96-97 1.0018623765310473 1104 98-99 0.9888795153074881 1104 100-101 1.0156771428725797 1104 102-103 0.840199534613177 1104 104-105 0.8329741113813753 1104 106-107 0.7929294951546488 1104 108-109 0.7526602791137584 1104 110-111 0.7284502149822707 1104 112-113 0.9025413412436656 1104 114-115 0.8533494053266111 1104 116-117 0.7802515000396042 1104 118-119 0.7646714628889697 1104 120-121 0.7118614097693445 1105 1 0.6082349334015795 1105 2 0.8660774540744818 1105 3 0.7144274352738655 1105 4 1.0024808531695655 1105 5 1.0510951253155056 1105 6 1.0785501817206793 1105 7 1.073622091044804 1105 8 1.0024893332447036 1105 9 1.1386016551319784 1105 10-11 1.0663237677352626 1105 12-13 0.952871682585716 1105 14-15 1.1174630876894938 1105 16-17 1.05880662715375 1105 18-19 1.0600890077636222 1105 20-21 1.1041219012536416 1105 22-23 1.2353669011333324 1105 24-25 1.1328008875306423 1105 26-27 1.1068161393973313 1105 28-29 1.133188118748329 1105 30-31 1.1786743470565355 1105 32-33 1.339301630977154 1105 34-35 1.2918250438844083 1105 36-37 1.0421186465306604 1105 38-39 1.1140509825054181 1105 40-41 1.2081115143754317 1105 42-43 1.2448040531217046 1105 44-45 1.1945410871680053 1105 46-47 1.2843160448437345 1105 48-49 1.163160035426607 1105 50-51 1.073881750773971 1105 52-53 1.2257539763640608 1105 54-55 1.2937402765177524 1105 56-57 1.4650842055987567 1105 58-59 1.4521583551295691 1105 60-61 1.422337199805952 1105 62-63 1.2426915034342443 1105 64-65 1.2370173919600873 1105 66-67 1.3568973930488752 1105 68-69 1.343825557946655 1105 70-71 1.0898711229439328 1105 72-73 1.070108175165089 1105 74-75 1.185929809570439 1105 76-77 1.2901394123751508 1105 78-79 1.3769836987854092 1105 80-81 1.3945722980104414 1105 82-83 1.0399767211336375 1105 84-85 1.0344776649067526 1105 86-87 1.017855591056204 1105 88-89 1.0872329330709825 1105 90-91 1.129800362642424 1105 92-93 1.1061361951919828 1105 94-95 1.142132637625231 1105 96-97 1.0034093348913196 1105 98-99 1.1589775156423805 1105 100-101 1.1971864297502925 1105 102-103 1.0962911863979237 1105 104-105 1.0107859126022127 1105 106-107 0.9332606148277058 1105 108-109 0.8443582118290642 1105 110-111 0.9207951806477048 1105 112-113 0.9460525044848858 1105 114-115 0.9952306079618509 1105 116-117 0.9220820681002451 1105 118-119 0.8921109557740898 1105 120-121 0.894881481731332 1106 1 0.5552884025779008 1106 2 0.900206737858845 1106 3 0.8371554822848779 1106 4 1.0607266931018486 1106 5 1.0200419636290974 1106 6 1.0779172067470384 1106 7 1.0309560312048234 1106 8 0.9656572830120282 1106 9 1.0787181693494503 1106 10-11 1.0718573631921018 1106 12-13 0.8506924788622925 1106 14-15 1.105131781285401 1106 16-17 1.2176812053378683 1106 18-19 1.2771983317837368 1106 20-21 1.1979169382616206 1106 22-23 1.3114001152852026 1106 24-25 1.1747283164170526 1106 26-27 1.1585828960688573 1106 28-29 1.2211850054129556 1106 30-31 1.2560598179834486 1106 32-33 1.2843856796758644 1106 34-35 1.3240059597675824 1106 36-37 1.1882898729873759 1106 38-39 1.1245877969972753 1106 40-41 1.3118637211833644 1106 42-43 1.297590855165474 1106 44-45 1.1568333458473958 1106 46-47 1.3410657104921384 1106 48-49 1.2528565900545416 1106 50-51 1.2018690109078207 1106 52-53 1.2626418028209443 1106 54-55 1.3589149910631662 1106 56-57 1.382761774471696 1106 58-59 1.3761309937686548 1106 60-61 1.64206263402626 1106 62-63 1.5655613714319685 1106 64-65 1.4804032577320072 1106 66-67 1.3581582764009994 1106 68-69 1.2759834120863722 1106 70-71 1.1539838179969237 1106 72-73 1.2785950277874463 1106 74-75 1.5036684833280098 1106 76-77 1.488974750653366 1106 78-79 1.546056765202355 1106 80-81 1.5585412502647706 1106 82-83 1.4690781080703808 1106 84-85 1.4413081340123135 1106 86-87 1.374259548940568 1106 88-89 1.4947974033104146 1106 90-91 1.3714462023999978 1106 92-93 1.279207416669454 1106 94-95 1.274207635463192 1106 96-97 1.108490125245222 1106 98-99 1.3406337768620489 1106 100-101 1.369566279377242 1106 102-103 1.0490008975757945 1106 104-105 1.092753551036008 1106 106-107 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2204 100-101 1.4057878009699856 2204 102-103 1.280864295263278 2204 104-105 1.258804493132832 2204 106-107 1.3512955464293377 2204 108-109 1.4605909862882314 2204 110-111 1.4360122627978065 2204 112-113 1.4249785311937622 2204 114-115 1.4791747362756702 2204 116-117 1.3601465196346894 2204 118-119 1.3502153220329802 2204 120-121 1.3030791779138937 2205 1 0.8441461341225782 2205 2 1.1991495571796769 2205 3 1.0817600015402569 2205 4 1.1920225009860985 2205 5 1.2688435819791977 2205 6 1.3004828341930406 2205 7 1.3044685126062703 2205 8 1.1216352528879057 2205 9 1.285006474117239 2205 10-11 1.2739198678054393 2205 12-13 1.141557860775599 2205 14-15 1.3404234436275502 2205 16-17 1.3591808394555613 2205 18-19 1.3222130588745031 2205 20-21 1.310329622031766 2205 22-23 1.3552610672686356 2205 24-25 1.3828689261309748 2205 26-27 1.2999711356035561 2205 28-29 1.365054050645604 2205 30-31 1.291906429928261 2205 32-33 1.3797030230967877 2205 34-35 1.5744983552981537 2205 36-37 1.2688408958654165 2205 38-39 1.3851928198326036 2205 40-41 1.4876694772071204 2205 42-43 1.4032198224031731 2205 44-45 1.4135144167491234 2205 46-47 1.4125468987459868 2205 48-49 1.492592607748783 2205 50-51 1.4967231282172193 2205 52-53 1.4654637614547 2205 54-55 1.4016186833458377 2205 56-57 1.4849751657267163 2205 58-59 1.4938070622180533 2205 60-61 1.5213032624370868 2205 62-63 1.4281599189185457 2205 64-65 1.5185732317024687 2205 66-67 1.5285217344815294 2205 68-69 1.4373768522830623 2205 70-71 1.4314754802641332 2205 72-73 1.414439241732488 2205 74-75 1.4121531685399589 2205 76-77 1.3915089118461594 2205 78-79 1.6184103008094688 2205 80-81 1.6357272799677425 2205 82-83 1.5491879364597771 2205 84-85 1.584602521081699 2205 86-87 1.5883453621116566 2205 88-89 1.6909271440903844 2205 90-91 1.5002858590214174 2205 92-93 0.7125022571106712 2205 94-95 1.3545667808910125 2205 96-97 1.4801463674821669 2205 98-99 1.7144957667297511 2205 100-101 1.935977159401947 2205 102-103 1.9060680839089912 2205 104-105 1.9656895008368807 2205 106-107 1.8798665217181245 2205 108-109 1.8971130626962882 2205 110-111 2.0310376616699237 2205 112-113 2.0341430777546243 2205 114-115 2.066353031133449 2205 116-117 2.026304023550992 2205 118-119 1.9848979735816812 2205 120-121 1.8773088211370776 2206 1 0.7531772160155263 2206 2 1.0474192558443747 2206 3 1.0294495186809876 2206 4 1.0729742818130603 2206 5 1.2026818838577356 2206 6 1.0847382314579832 2206 7 1.1650928781586174 2206 8 1.0438692912781775 2206 9 0.8478156414687064 2206 10-11 1.077622999243438 2206 12-13 1.0318220096777324 2206 14-15 1.1543728328314131 2206 16-17 1.1451795147403487 2206 18-19 1.1409887388988054 2206 20-21 1.209926107565785 2206 22-23 1.283507655350114 2206 24-25 1.1934623030153304 2206 26-27 1.2340382990096472 2206 28-29 1.1348294820700104 2206 30-31 1.2067605904086989 2206 32-33 1.2476871541365497 2206 34-35 1.2676918109058093 2206 36-37 1.1136215927737965 2206 38-39 1.2197442351256456 2206 40-41 1.318457510114385 2206 42-43 1.215973802878871 2206 44-45 1.3461272419718853 2206 46-47 1.347240942476546 2206 48-49 1.4373294256240357 2206 50-51 1.3645527187409598 2206 52-53 1.3553878694299186 2206 54-55 1.3962824174544366 2206 56-57 1.6395549283138067 2206 58-59 1.5130351710403573 2206 60-61 1.5128442192704767 2206 62-63 1.3750933355788817 2206 64-65 1.5508028850667372 2206 66-67 1.5585430496210364 2206 68-69 1.4644648900242139 2206 70-71 1.4182768109641515 2206 72-73 1.2565283555889408 2206 74-75 1.4034786721273953 2206 76-77 1.571478061289227 2206 78-79 1.6203738371890744 2206 80-81 1.6087745047411843 2206 82-83 1.5710356379465402 2206 84-85 1.4910652552662569 2206 86-87 1.5447893649843678 2206 88-89 1.6161560919373485 2206 90-91 1.4872706184664857 2206 92-93 1.4637412859137306 2206 94-95 1.4833742724950092 2206 96-97 1.4412837868310682 2206 98-99 1.5402800073376248 2206 100-101 1.5565726827622761 2206 102-103 1.5523938407000273 2206 104-105 1.5811149231689754 2206 106-107 1.6705214673599151 2206 108-109 1.7503690308202557 2206 110-111 1.6023917600003905 2206 112-113 1.5295664226662673 2206 114-115 1.1425928080380565 2206 116-117 0.5294188306673959 2206 118-119 0.816107676804041 2206 120-121 0.7712836975852326 2207 1 0.5305245274518988 2207 2 0.9822481583876552 2207 3 0.8829648359648132 2207 4 1.1444309411704126 2207 5 1.1891173513570976 2207 6 1.0515334568975092 2207 7 1.0672639136281816 2207 8 1.0164193136193802 2207 9 1.2076991701907147 2207 10-11 1.059968121758459 2207 12-13 0.9426544584027354 2207 14-15 1.1131155396554178 2207 16-17 1.099533563405437 2207 18-19 1.279846096128189 2207 20-21 1.2876153814637732 2207 22-23 1.2780889450444874 2207 24-25 1.1917755744648204 2207 26-27 1.1292361025981918 2207 28-29 1.1508654294532548 2207 30-31 1.2561580559089762 2207 32-33 1.3266135882997432 2207 34-35 1.2998235943020333 2207 36-37 1.2442922873140176 2207 38-39 1.3559348533700089 2207 40-41 1.4984464105397706 2207 42-43 1.4636596158274742 2207 44-45 1.4604310681289192 2207 46-47 1.5261863924903665 2207 48-49 1.5651107762724479 2207 50-51 1.5150935243673302 2207 52-53 1.379596435537934 2207 54-55 1.307600109362788 2207 56-57 1.529033771323597 2207 58-59 1.1760500841162909 2207 60-61 1.362861620872323 2207 62-63 1.405444763995341 2207 64-65 1.5251547318964853 2207 66-67 1.555645795910202 2207 68-69 1.0759179528352796 2207 70-71 1.0715456970291797 2207 72-73 1.3481337453719107 2207 74-75 1.5348499506382538 2207 76-77 1.5459240188228627 2207 78-79 1.5475728314475141 2207 80-81 1.713739094579939 2207 82-83 1.6194022441757348 2207 84-85 1.4776595648455952 2207 86-87 1.4853838798961512 2207 88-89 1.5859652812745288 2207 90-91 1.4671021942057472 2207 92-93 1.3956680919998377 2207 94-95 1.2014389431753436 2207 96-97 1.3242015156734297 2207 98-99 1.4472770009606961 2207 100-101 1.5969776525822823 2207 102-103 1.5257847252308778 2207 104-105 1.4525030415231122 2207 106-107 1.4284835880437363 2207 108-109 1.5385750709517723 2207 110-111 1.5776979507090338 2207 112-113 1.5311399923772768 2207 114-115 1.6659093402700869 2207 116-117 1.5667696417951191 2207 118-119 1.5527278143638625 2207 120-121 1.1996680347230253 2208 1 0.2588757243703981 2208 2 0.8598656293433251 2208 3 0.7135465811666428 2208 4 0.9836865141633311 2208 5 1.169058259713509 2208 6 1.110672117086672 2208 7 1.0377775299742211 2208 8 1.0080980286311032 2208 9 1.0842727418039715 2208 10-11 1.10558572215961 2208 12-13 0.9562635040703 2208 14-15 1.247753221430706 2208 16-17 1.251135304120254 2208 18-19 1.303433258100334 2208 20-21 1.310959642479908 2208 22-23 1.2799558528632886 2208 24-25 1.22249623929158 2208 26-27 1.2011672014279569 2208 28-29 1.1666725966576976 2208 30-31 1.1431479570912941 2208 32-33 1.2094312426582903 2208 34-35 1.3218153788725076 2208 36-37 1.1548234507139838 2208 38-39 1.1870550013301155 2208 40-41 1.3348688926917163 2208 42-43 1.2366281526217549 2208 44-45 1.1742531540338632 2208 46-47 1.0108613770097534 2208 48-49 1.284772645720146 2208 50-51 1.21484971903633 2208 52-53 1.3452653804668273 2208 54-55 1.1720692210889396 2208 56-57 1.1704691386398522 2208 58-59 1.19334601053896 2208 60-61 1.2380560206337492 2208 62-63 1.2196929521672217 2208 64-65 1.304546755461665 2208 66-67 1.2519775420109553 2208 68-69 1.2930513042343215 2208 70-71 1.2081567413654568 2208 72-73 1.1962478892627608 2208 74-75 1.2416942114909446 2208 76-77 1.3646234752934951 2208 78-79 1.2601269127992047 2208 80-81 1.3965498898535245 2208 82-83 1.3036941441684213 2208 84-85 1.328186237969284 2208 86-87 1.3910449295224758 2208 88-89 1.5032767509612377 2208 90-91 1.540253382058708 2208 92-93 1.6547004009596122 2208 94-95 1.6368904044437436 2208 96-97 1.6418053436421296 2208 98-99 1.7708287016269573 2208 100-101 1.6246183213011172 2208 102-103 1.5563013336416276 2208 104-105 1.6109827446789176 2208 106-107 1.6417522073540631 2208 108-109 1.4758988880810513 2208 110-111 1.4450796946904987 2208 112-113 1.5945064421791777 2208 114-115 1.6122450362013474 2208 116-117 1.5009545422414128 2208 118-119 1.5427256994339302 2208 120-121 1.3830512830178705 2209 1 0.6381673820443403 2209 2 1.0011846488576772 2209 3 1.0468798357028177 2209 4 1.1292980482707193 2209 5 1.1891704463795634 2209 6 1.1572082018906968 2209 7 1.1716299474670961 2209 8 1.0165587749993605 2209 9 1.204387918822185 2209 10-11 1.1289227317743595 2209 12-13 1.0951834160570186 2209 14-15 1.2839578168336985 2209 16-17 1.2877992968053604 2209 18-19 1.3236813698643317 2209 20-21 1.3011931582522962 2209 22-23 1.351068111392351 2209 24-25 1.208686397085252 2209 26-27 1.2260667586903722 2209 28-29 1.2002639138569933 2209 30-31 1.3861606387847445 2209 32-33 1.3198351624071734 2209 34-35 1.415229045514664 2209 36-37 1.2607322683760245 2209 38-39 1.3654775243788748 2209 40-41 1.4612638387838217 2209 42-43 1.4530553921532103 2209 44-45 1.5203805652564526 2209 46-47 1.4472881744891595 2209 48-49 1.5456051373028359 2209 50-51 1.5188341150714777 2209 52-53 1.5589678832142937 2209 54-55 1.4284039216317694 2209 56-57 1.3485926560220705 2209 58-59 1.3543309391641003 2209 60-61 1.3831636272149836 2209 62-63 1.4349187615775492 2209 64-65 1.4709366569597009 2209 66-67 1.5864461257021674 2209 68-69 1.4351774233411057 2209 70-71 1.5075722811736654 2209 72-73 1.3649306107716228 2209 74-75 1.4657643419861337 2209 76-77 1.3795807912668714 2209 78-79 1.11402681661151 2209 80-81 1.1986386035553203 2209 82-83 1.2613394404154015 2209 84-85 1.3679697116521723 2209 86-87 1.4321543229868645 2209 88-89 1.5633600174942792 2209 90-91 1.4925161035250163 2209 92-93 1.4628909696016699 2209 94-95 1.607286745481943 2209 96-97 1.4826692327404878 2209 98-99 1.5697513356615183 2209 100-101 1.6828857467213787 2209 102-103 1.5844332783799224 2209 104-105 1.740070957939956 2209 106-107 1.4983566955771543 2209 108-109 1.749315591171701 2209 110-111 1.8209326246800899 2209 112-113 1.9125099459736177 2209 114-115 1.9948651744856285 2209 116-117 1.8334683161816656 2209 118-119 1.868710784077443 2209 120-121 1.763663187077917 2210 1 0.4681047465855137 2210 2 0.7028938557323805 2210 3 0.7007638654143307 2210 4 0.882618966737077 2210 5 0.9814082319974915 2210 6 0.8527868960150258 2210 7 0.8432377779515576 2210 8 0.7340673613645166 2210 9 0.772624212785658 2210 10-11 0.8573141414345784 2210 12-13 0.787847129809002 2210 14-15 0.8159103176929179 2210 16-17 0.8227069919186647 2210 18-19 0.861422448578324 2210 20-21 0.8885935295292953 2210 22-23 0.9947869719366906 2210 24-25 0.7938257896218275 2210 26-27 0.7150347699502575 2210 28-29 0.9277492252690891 2210 30-31 0.9491058847791365 2210 32-33 0.8817927133817776 2210 34-35 0.9098362353826346 2210 36-37 0.8387366778749126 2210 38-39 0.9273125048447497 2210 40-41 0.9455842241824399 2210 42-43 0.9498670976660222 2210 44-45 1.0572937523702421 2210 46-47 1.0283310116252764 2210 48-49 0.9649153406401254 2210 50-51 0.8840753516015809 2210 52-53 0.959666365607859 2210 54-55 0.7614404437856379 2210 56-57 0.8962269341478262 2210 58-59 0.7704849372890124 2210 60-61 0.861926956830871 2210 62-63 0.8561982176614151 2210 64-65 0.9394021349788417 2210 66-67 1.069430393189947 2210 68-69 0.9959033072866497 2210 70-71 0.9574124193036724 2210 72-73 0.9575174037846921 2210 74-75 1.0359116720883286 2210 76-77 1.0593124103665943 2210 78-79 0.7742170610043608 2210 80-81 0.8326636974851276 2210 82-83 0.6958499065457247 2210 84-85 0.8076150462573892 2210 86-87 0.8059076926529727 2210 88-89 1.0098439915610022 2210 90-91 0.9046248657090032 2210 92-93 1.0902440661874628 2210 94-95 1.0969478032473141 2210 96-97 0.9863419642216478 2210 98-99 0.8869387954768619 2210 100-101 1.0084938063314475 2210 102-103 0.9700213947102654 2210 104-105 1.06989697445103 2210 106-107 0.9446499459576678 2210 108-109 1.1659737240785795 2210 110-111 0.9773253459674969 2210 112-113 1.252745571264409 2210 114-115 1.138403189462963 2210 116-117 1.1232146530945002 2210 118-119 1.2323026085017652 2210 120-121 1.2439554625055997 2211 1 0.30671350624690774 2211 2 0.6190889093887364 2211 3 0.5573942319518181 2211 4 0.8186852740108108 2211 5 0.9409685890810664 2211 6 0.7539884808079407 2211 7 0.8272123173440562 2211 8 0.7022452103473213 2211 9 0.6621599608353392 2211 10-11 0.690442519764197 2211 12-13 0.6228648468700584 2211 14-15 0.8292197636649021 2211 16-17 0.6600048333960729 2211 18-19 0.7819183097862634 2211 20-21 0.8537450446808137 2211 22-23 0.4053883416711912 2211 24-25 0.7253253530099428 2211 26-27 0.7042320535747919 2211 28-29 0.7504598995277476 2211 30-31 0.7266322799525895 2211 32-33 0.8241675212364541 2211 34-35 0.8999478781258574 2211 36-37 0.6277376665663112 2211 38-39 0.7119798498836118 2211 40-41 0.8348201172883023 2211 42-43 0.8153830574544259 2211 44-45 0.6858200248593604 2211 46-47 0.8538302286105619 2211 48-49 0.8368025865211806 2211 50-51 0.6547390016769938 2211 52-53 0.6984025004740246 2211 54-55 0.7600678225987778 2211 56-57 0.6704917302392488 2211 58-59 0.533856447100149 2211 60-61 0.7458877844449461 2211 62-63 0.6888257552425188 2211 64-65 0.7483819064973076 2211 66-67 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-0.926238386133118 2316 106-107 -0.7260037920135822 2316 108-109 -1.0510489298470453 2316 110-111 -1.1173320793147425 2316 112-113 -1.3750558152933614 2316 114-115 -1.232211406714864 2316 116-117 -0.9325615622791901 2316 118-119 -1.135402260975411 2316 120-121 -1.5045383836902602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55518.0 3 9769.0 4 8452.0 5 9538.0 6 10937.0 7 11739.0 8 13494.0 9 15132.0 10 17866.0 11 20313.0 12 21706.0 13 24437.0 14 28900.0 15 30460.0 16 26368.0 17 26574.0 18 28643.0 19 30400.0 20 33211.0 21 36570.0 22 40779.0 23 45501.0 24 51339.0 25 58683.0 26 67265.0 27 77576.0 28 89262.0 29 104788.0 30 122822.0 31 147829.0 32 179913.0 33 224187.0 34 283953.0 35 366592.0 36 479711.0 37 526687.0 38 186371.0 39 770.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.98071135778048 18.150289577683886 13.640957885856242 20.228041178679394 2 13.48297812098922 23.96107395861629 36.45718983415759 26.098758086236906 3 26.41814480218157 23.770149663915937 29.868159377035663 19.943546156866827 4 11.004402830226637 15.220974945252014 44.11538174147454 29.659240483046805 5 10.83559494621131 40.50624107437701 35.18459388253714 13.473570096874536 6 26.19931046654751 38.041734193305075 17.586006276743568 18.17294906340385 7 25.046030301401846 33.57670169864528 23.460265942634763 17.917002057318108 8 22.342324220798535 28.426108583177324 20.3104305884618 28.921136607562346 9 20.605767343709996 22.663102867350865 26.828259794533675 29.902869994405464 10-11 25.262296730448774 23.55698730104005 27.947250474056197 23.23346549445498 12-13 30.53697937001952 22.31633134724486 26.15557568554241 20.991113597193216 14-15 23.167794975987754 25.36367433901682 31.025657305778914 20.442873379216508 16-17 19.41342483845698 29.02784235539292 30.216849556220076 21.341883249930028 18-19 18.269089943237425 33.84671129043919 27.91049240274308 19.97370636358031 20-21 21.67996922297349 27.325360565885703 25.39553063425019 25.599139576890618 22-23 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27.19852338335565 19.39540732681638 48-49 23.356455378418737 27.209368020424186 28.238207067310846 21.195969533846238 50-51 17.596741227140637 28.627931620370205 33.077492842697396 20.697834309791766 52-53 20.9182961651402 31.116336068533407 26.22945708321222 21.73591068311417 54-55 18.877169768155696 27.00175087457507 28.56434607766158 25.556733279607656 56-57 23.276578664150968 24.78910363257294 27.911453860138884 24.02286384313721 58-59 27.484833707235683 25.034368402542277 26.256424717424537 21.2243731727975 60-61 22.629910160527125 26.65956924720539 32.040708866029114 18.66981172623837 62-63 20.02778349395471 27.922382683930923 30.458822832875974 21.591010989238388 64-65 18.839532643820167 33.1066615915187 27.288073416642206 20.765732348018933 66-67 21.373846164015347 27.51612331226475 25.21379680186367 25.896233721856234 68-69 19.250395389126854 28.441545283462204 27.241876109730274 25.066183217680667 70-71 24.356976851640933 27.20693541031938 25.904986626245268 22.531101111794413 72-73 22.507867546958074 24.40629771694538 31.933056575050134 21.152778161046413 74-75 19.13278968409911 32.40071914174446 27.586082985898603 20.88040818825783 76-77 20.827175841856068 26.928770713609655 26.804897318917998 25.439156125616275 78-79 22.074828767271104 27.424011744796978 28.450731906075006 22.050427581856912 80-81 25.036434442669165 25.926235948487097 27.34378854057854 21.693541068265194 82-83 22.378540519771324 24.841959865969596 31.612385541828974 21.167114072430103 84-85 21.072987607229095 28.512029199586635 29.8888120255042 20.526171167680072 86-87 20.98143325683144 29.922666722252135 27.7448282157569 21.35107180515953 88-89 19.345996480340133 29.437216679887197 25.096437012837473 26.1203498269352 90-91 21.295503835401572 25.43966404024386 26.66569862103817 26.599133503316395 92-93 24.21958967375172 28.35221928838394 27.647250004563904 19.78094103330043 94-95 22.440214131389556 27.039111621801066 28.4691600748997 22.051514171909677 96-97 17.14079489433102 28.397034880093656 32.603352392189805 21.858817833385515 98-99 20.493680366683694 30.586412615796664 26.563887776750008 22.35601924076963 100-101 18.382398721977655 26.537484030112896 28.98824429379847 26.091872954110972 102-103 22.485430289272525 24.886401320621033 28.446063788330346 24.182104601776093 104-105 26.13192559502679 25.32214359402132 26.595742234534963 21.950188576416924 106-107 21.763766766593836 26.548586077018754 31.543243820473734 20.144403335913672 108-109 19.621671466029536 27.385170284920612 30.62492706099052 22.368231188059333 110-111 18.72728299724374 31.779233671748912 27.195294827152793 22.29818850385455 112-113 21.26054680161858 27.099374612016113 25.13465798703863 26.50542059932668 114-115 19.23884551029786 27.22296733135256 27.316280451634352 26.221906706715224 116-117 23.49685243768931 26.558532926253786 26.211698619650647 23.73291601640626 118-119 22.07902879602643 24.276476130459095 31.190809242230337 22.453685831284137 120-121 19.323849125078628 31.65856160327315 27.031790285653173 21.985798985995046 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8938.0 1 11183.5 2 7759.5 3 1991.0 4 1777.5 5 1685.0 6 1809.5 7 2001.0 8 2288.5 9 2442.5 10 2286.5 11 2182.0 12 2245.5 13 2400.0 14 2617.5 15 2784.5 16 2952.5 17 3197.0 18 3495.5 19 3829.0 20 4171.0 21 4689.5 22 5556.0 23 6708.0 24 8276.0 25 10441.5 26 13326.0 27 17214.5 28 22587.5 29 29282.5 30 36268.0 31 43572.5 32 51343.5 33 58211.5 34 64132.5 35 70700.0 36 77464.0 37 84997.5 38 91573.0 39 94947.5 40 97478.5 41 99932.5 42 102465.5 43 105939.0 44 108243.5 45 112996.5 46 131452.0 47 231712.0 48 448674.0 49 441274.0 50 224335.5 51 119578.0 52 82606.5 53 66123.0 54 56157.0 55 47609.0 56 39349.5 57 31430.0 58 25072.0 59 20239.5 60 16257.0 61 12627.5 62 9519.5 63 6963.0 64 4842.5 65 3298.5 66 2229.0 67 1475.0 68 960.0 69 647.0 70 451.0 71 313.0 72 231.0 73 167.0 74 115.5 75 88.5 76 65.0 77 46.0 78 31.5 79 33.0 80 30.0 81 20.0 82 13.5 83 10.5 84 8.5 85 11.0 86 11.0 87 7.0 88 5.0 89 3.5 90 5.5 91 7.0 92 4.5 93 2.5 94 4.0 95 3.5 96 4.5 97 5.0 98 2.5 99 2.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19083366651916375 2 1.0002689200937378 3 1.0091190946072273 4 0.981060341969605 5 0.9847882289833255 6 1.0011510918298092 7 0.9755681114837418 8 0.9689660520395953 9 0.9639575931509325 10-11 0.9520340461375818 12-13 0.9488753021793911 14-15 0.9485053591932966 16-17 0.9725801104422098 18-19 1.0035841783921993 20-21 0.9913476026983072 22-23 0.9672870800257822 24-25 0.9578535338803746 26-27 0.9615956494704835 28-29 0.9922724601635433 30-31 0.9972097761702648 32-33 0.9876624014137513 34-35 0.9959576614480992 36-37 1.0078100655795086 38-39 0.9955023470036752 40-41 1.0057753791559894 42-43 0.999415205510443 44-45 0.976222625997601 46-47 0.9734622821782812 48-49 0.9749135969698823 50-51 0.9737326251296579 52-53 0.9725374247130452 54-55 0.9860688008582678 56-57 1.0290675587035492 58-59 1.0018482920728333 60-61 1.0186380122109642 62-63 0.9860688008582678 64-65 0.9776027979072609 66-67 0.9808042275946165 68-69 0.9927989174899083 70-71 0.9971244047119354 72-73 1.001890977801998 74-75 1.0092044660655568 76-77 1.0218109847455432 78-79 1.1045359278668092 80-81 1.070145458736417 82-83 1.0892402082494441 84-85 1.045672307348633 86-87 1.0223943563774613 88-89 1.020985727315025 90-91 1.031785216793704 92-93 1.0152516110305616 94-95 1.0112676096418525 96-97 1.0210995559261309 98-99 1.06062654113268 100-101 1.0474366508207755 102-103 1.015678468322209 104-105 0.9986753195382543 106-107 1.0013502918992447 108-109 0.9994436626632196 110-111 1.002986578183893 112-113 1.0055192647810007 114-115 1.0086495515864151 116-117 1.041588705925206 118-119 1.0379604189462033 120-121 1.0122493814126416 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 3514055.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.654331564598294 #Duplication Level Percentage of deduplicated Percentage of total 1 79.39441489095533 36.24698941808955 2 12.71337679574186 11.608414390769381 3 3.4044893777227916 4.662890605761277 4 1.4429885239690505 2.635147060687733 5 0.754082007176537 1.7213554991267705 6 0.4675963367433242 1.2808678917642766 7 0.31952407559384854 1.0211360663023332 8 0.22792821310829622 0.832472817133806 9 0.17940285644350523 0.7371465742537036 >10 0.9835385483278629 8.164444220630894 >50 0.06929196290182148 2.1723202934095758 >100 0.03521798563178348 2.984654329598361 >500 0.0033478028003644652 1.0343345698494746 >1k 0.0029056401663306386 2.9316479782432987 >5k 6.316609057240519E-4 1.8685907041998522 >10k+ 0.0012633218114481038 20.097587580179756 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 140180 3.9891236762088242 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 105566 3.0041077900032866 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 67988 1.934744902968223 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 58324 1.6597349785361926 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 38183 1.0865794644648419 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 36332 1.0339052746755528 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 29380 0.8360711485733718 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 26757 0.76142803684063 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 25691 0.7310927119808882 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 24208 0.6888907544133487 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 21474 0.6110888987224161 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 19575 0.5570487655998555 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 15215 0.4329755794943448 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 15125 0.4304144357444605 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 14635 0.4164704308839788 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 13222 0.3762604740107938 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 12035 0.3424818336650963 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 11374 0.3236716556798343 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 11000 0.3130286805414258 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 10648 0.3030117627641002 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9603 0.2732740381126647 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 9189 0.2614927768631965 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 7838 0.2230471634621541 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5961 0.16963308770067628 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5554 0.15805102652064354 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 5536 0.15753879777066665 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5453 0.1551768540902177 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 5317 0.15130668131261463 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5288 0.15048142388209632 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 5057 0.14390782159072638 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 4912 0.13978153443813487 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4821 0.137191933535474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4233 0.12045912770289594 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4188 0.11917855582795375 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3963 0.11277569645324276 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3820 0.10870632360620422 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8457152776493254E-5 0.0 5 0.0 0.0 0.0 2.8457152776493254E-5 0.0 6 0.0 0.0 0.0 2.8457152776493254E-5 0.0 7 0.0 0.0 0.0 2.8457152776493254E-5 0.0 8 0.0 0.0 0.0 2.8457152776493254E-5 0.0 9 0.0 0.0 0.0 5.691430555298651E-5 0.0 10-11 0.0 0.0 0.0 1.1382861110597302E-4 0.0 12-13 0.0 0.0 0.0 2.561143749884393E-4 0.0 14-15 0.0 0.0 0.0 3.841715624826589E-4 0.0 16-17 0.0 0.0 0.0 5.122287499768785E-4 0.0 18-19 0.0 0.0 0.0 7.683431249653179E-4 0.0 20-21 0.0 0.0 0.0 0.0011098289582832368 0.0 22-23 0.0 2.8457152776493254E-5 0.0 0.0017358863193660885 0.0 24-25 0.0 2.8457152776493254E-5 0.0 0.002518458020719653 0.0 26-27 0.0 2.8457152776493254E-5 0.0 0.0030733724998612713 0.0 28-29 0.0 2.8457152776493254E-5 0.0 0.0045246872914624274 0.0 30-31 0.0 4.268572916473988E-5 0.0 0.007555374062158959 0.0 32-33 0.0 5.691430555298651E-5 0.0 0.011966232742515414 0.0 34-35 0.0 5.691430555298651E-5 0.0 0.016334405693707126 0.0 36-37 0.0 8.537145832947976E-5 0.0 0.019962692672710017 0.0 38-39 0.0 1.1382861110597302E-4 0.0 0.026209037707150287 0.0 40-41 1.4228576388246627E-5 1.1382861110597302E-4 0.0 0.043653272359140655 0.0 42-43 2.8457152776493254E-5 1.1382861110597302E-4 0.0 0.05617441958079768 0.0 44-45 2.8457152776493254E-5 1.1382861110597302E-4 0.0 0.07077293895513873 0.0 46-47 2.8457152776493254E-5 1.1382861110597302E-4 0.0 0.10994420975198169 0.0 48-49 2.8457152776493254E-5 1.1382861110597302E-4 0.0 0.12535375798045278 0.0 50-51 2.8457152776493254E-5 1.1382861110597302E-4 0.0 0.14383667870878514 0.0 52-53 2.8457152776493254E-5 1.1382861110597302E-4 0.0 0.16391319999260112 0.0 54-55 2.8457152776493254E-5 1.1382861110597302E-4 0.0 0.1926691528732476 0.0 56-57 2.8457152776493254E-5 1.1382861110597302E-4 0.0 0.326830399638025 0.0 58-59 2.8457152776493254E-5 1.1382861110597302E-4 0.0 0.41302711539802306 0.0 60-61 2.8457152776493254E-5 1.2805718749421964E-4 0.0 0.45961147449314255 0.0 62-63 2.8457152776493254E-5 1.4228576388246626E-4 0.0 0.5214061817472977 0.0 64-65 2.8457152776493254E-5 1.4228576388246626E-4 0.0 0.5798571735502148 0.0 66-67 2.8457152776493254E-5 1.4228576388246626E-4 0.0 0.7483804322926078 0.0 68-69 2.8457152776493254E-5 1.4228576388246626E-4 0.0 0.9209446067292629 0.0 70-71 2.8457152776493254E-5 1.4228576388246626E-4 0.0 0.9613822208246598 0.0 72-73 2.8457152776493254E-5 1.4228576388246626E-4 0.0 0.991859831448284 0.0 74-75 2.8457152776493254E-5 1.4228576388246626E-4 0.0 1.1211549050882812 0.0 76-77 2.8457152776493254E-5 1.4228576388246626E-4 0.0 1.2448439196313092 0.0 78-79 2.8457152776493254E-5 1.4228576388246626E-4 0.0 1.3482572128210857 0.0 80-81 2.8457152776493254E-5 1.4228576388246626E-4 0.0 1.4347242715324602 0.0 82-83 2.8457152776493254E-5 1.4228576388246626E-4 0.0 1.6479110315575596 0.0 84-85 4.268572916473988E-5 1.4228576388246626E-4 0.0 2.2176659158721193 0.0 86-87 5.691430555298651E-5 1.4228576388246626E-4 0.0 2.7190951763703186 0.0 88-89 5.691430555298651E-5 1.4228576388246626E-4 0.0 3.3395607069325894 0.0 90-91 5.691430555298651E-5 1.4228576388246626E-4 0.0 4.029689347491715 0.0 92-93 5.691430555298651E-5 1.4228576388246626E-4 0.0 4.7514481133619135 0.0 94-95 5.691430555298651E-5 1.4228576388246626E-4 0.0 5.340909575974195 0.0 96-97 5.691430555298651E-5 1.4228576388246626E-4 0.0 6.001371634763827 0.0 98-99 5.691430555298651E-5 1.9920006943545277E-4 0.0 6.7044482798362575 0.0 100-101 5.691430555298651E-5 1.9920006943545277E-4 0.0 7.467911003100407 0.0 102-103 5.691430555298651E-5 1.9920006943545277E-4 0.0 8.245659217058357 0.0 104-105 5.691430555298651E-5 1.9920006943545277E-4 0.0 8.842149596406431 0.0 106-107 5.691430555298651E-5 1.9920006943545277E-4 0.0 9.500818854571143 0.0 108-109 5.691430555298651E-5 1.9920006943545277E-4 0.0 10.310524451097095 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3305 0.0 47.53696 2 ACCTGGG 3450 0.0 44.64518 3 GTACCTG 4385 0.0 36.246105 1 TATAACG 480 0.0 35.968327 2 AGTACGC 2635 0.0 32.97893 2 GTACGCA 2710 0.0 32.02333 3 GAGTACG 2855 0.0 31.667616 1 TACGCAG 2950 0.0 30.19429 4 CCTGGGG 2850 0.0 28.229183 4 ACGCAGT 3295 0.0 26.684399 5 ATAACGC 755 0.0 26.642805 3 GTACCGG 285 5.456968E-12 26.267595 1 CGCAGTG 3505 0.0 25.41573 6 GTATAAC 995 0.0 24.886694 1 GTACCCG 445 0.0 24.587559 1 TAACGCA 810 0.0 24.121761 4 GTACACG 540 0.0 23.461227 1 TACCGGG 330 4.5474735E-11 22.670944 2 GTCGCCG 505 0.0 22.198835 112-113 GAGTACT 7390 0.0 21.270363 12-13 >>END_MODULE