##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003826_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3514055 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7972029464536 31.0 31.0 34.0 30.0 34.0 2 31.96857846561878 31.0 31.0 34.0 30.0 34.0 3 32.038015056679534 33.0 31.0 34.0 30.0 34.0 4 35.724968163560334 37.0 35.0 37.0 35.0 37.0 5 35.60263740891933 37.0 35.0 37.0 33.0 37.0 6 35.69310212845274 37.0 35.0 37.0 33.0 37.0 7 35.655972089224555 37.0 35.0 37.0 33.0 37.0 8 35.667724324178195 37.0 35.0 37.0 33.0 37.0 9 37.25986531229591 39.0 37.0 39.0 33.0 39.0 10-11 37.1623306977267 39.0 37.0 39.0 33.0 39.0 12-13 37.142322473609546 39.0 37.0 39.0 33.0 39.0 14-15 38.205009739460536 40.0 37.5 41.0 33.0 41.0 16-17 38.280189979951935 40.0 38.0 41.0 33.0 41.0 18-19 38.343551964895255 40.0 38.0 41.0 33.5 41.0 20-21 38.27352446105709 40.0 38.0 41.0 33.5 41.0 22-23 38.18173321134701 40.0 37.5 41.0 33.0 41.0 24-25 38.014844673745856 40.0 37.0 41.0 33.0 41.0 26-27 37.79163075136843 39.5 37.0 41.0 32.5 41.0 28-29 37.705825321459116 39.5 37.0 41.0 33.0 41.0 30-31 37.57320488723141 39.0 37.0 41.0 32.0 41.0 32-33 37.3252948516742 39.0 36.0 41.0 31.5 41.0 34-35 37.227214144343215 39.0 36.0 41.0 31.0 41.0 36-37 37.064715264843606 39.0 35.5 41.0 30.5 41.0 38-39 36.840505484404765 39.0 35.5 40.0 30.0 41.0 40-41 37.021920687069496 39.0 36.0 41.0 31.0 41.0 42-43 36.91926563471545 39.0 36.0 41.0 30.5 41.0 44-45 36.68998052107892 39.0 36.0 41.0 30.0 41.0 46-47 36.56235047544788 39.0 36.0 41.0 30.0 41.0 48-49 36.28852095371302 39.0 35.0 41.0 30.0 41.0 50-51 36.04882023758877 39.0 35.0 40.0 28.5 41.0 52-53 35.965379454789414 39.0 35.0 40.0 28.0 41.0 54-55 35.81868482422728 39.0 35.0 40.0 28.0 41.0 56-57 35.46069099658372 38.5 34.5 40.0 27.5 41.0 58-59 35.16450880820021 38.0 34.0 40.0 27.0 41.0 60-61 34.666679946671294 38.0 34.0 40.0 26.0 41.0 62-63 34.527015513416835 37.5 34.0 40.0 26.0 41.0 64-65 34.12230656606115 37.0 33.0 39.5 25.5 41.0 66-67 33.68221271437129 36.5 33.0 39.0 25.0 40.5 68-69 33.2790960869992 36.0 32.0 39.0 24.5 40.0 70-71 32.726616117277615 35.0 31.5 38.5 23.0 40.0 72-73 32.25965401793654 35.0 31.5 37.0 22.5 39.5 74-75 31.918955878607477 35.0 31.0 37.0 22.0 39.0 76-77 30.919684524004317 34.0 30.5 36.0 20.5 38.5 78-79 31.342017412931785 34.0 31.0 36.0 21.0 38.5 80-81 31.032701963970396 34.0 31.0 36.0 20.0 37.0 82-83 30.689039443036606 34.0 30.5 35.0 19.0 37.0 84-85 30.33283656630303 34.0 30.0 35.0 18.0 36.5 86-87 30.217198791709293 34.0 30.5 35.0 18.0 36.0 88-89 29.907660523241667 34.0 30.0 35.0 13.0 36.0 90-91 29.533353917340506 34.0 29.5 35.0 7.0 35.5 92-93 29.299967416560072 34.0 29.0 35.0 3.5 35.0 94-95 28.90431680779043 34.0 29.0 35.0 2.0 35.0 96-97 28.50668316232956 34.0 29.0 35.0 2.0 35.0 98-99 28.246340908153115 34.0 29.0 35.0 2.0 35.0 100-101 27.28498301819408 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.08754900270539778 1101 2 0.11396443314359672 1101 3 0.12654777085283087 1101 4 0.10002821476950885 1101 5 0.12768150267888245 1101 6 0.08521277983881248 1101 7 0.0781130861886794 1101 8 0.04887121397690919 1101 9 0.08233695850448441 1101 10-11 0.16198997759858713 1101 12-13 0.17532912220559638 1101 14-15 0.2575158644338842 1101 16-17 0.222612202732563 1101 18-19 0.16379401155075612 1101 20-21 0.053499791352834336 1101 22-23 -0.003515782331646733 1101 24-25 0.2123650851681731 1101 26-27 0.17347773939850697 1101 28-29 0.20181300663505652 1101 30-31 0.24003993535806245 1101 32-33 0.4195657341192742 1101 34-35 0.3011731048399753 1101 36-37 0.29625151341286227 1101 38-39 0.2782075555845722 1101 40-41 0.35406077702230476 1101 42-43 0.40616158047140516 1101 44-45 0.38596303185215675 1101 46-47 0.25575283085927936 1101 48-49 0.22292515351487197 1101 50-51 0.15997782746791955 1101 52-53 0.0865917310080988 1101 54-55 0.25175980330798353 1101 56-57 0.20459566232729287 1101 58-59 0.19214067894579756 1101 60-61 0.09821229126522013 1101 62-63 0.06220734114185689 1101 64-65 -0.06432078828137833 1101 66-67 -0.015504206754293648 1101 68-69 0.08621883501647432 1101 70-71 0.10071370156716597 1101 72-73 0.009643987055000025 1101 74-75 0.012436007263598725 1101 76-77 0.020379883058023296 1101 78-79 0.08150357619295434 1101 80-81 -0.051961751347580076 1101 82-83 0.040663623411823835 1101 84-85 0.09731079439073298 1101 86-87 -0.03832575506567437 1101 88-89 0.043890693595280084 1101 90-91 0.14047281117222354 1101 92-93 0.14911524200259763 1101 94-95 0.3158362348937871 1101 96-97 0.18695891968617673 1101 98-99 -0.023564176517638202 1101 100-101 0.023200186923663324 1102 1 0.004617060143335294 1102 2 0.07101156038887879 1102 3 0.10479272273054363 1102 4 0.05377420457890736 1102 5 0.05078191613596772 1102 6 0.036293086586226764 1102 7 -0.008046909693902649 1102 8 -0.05351997475509762 1102 9 -0.027435039642682568 1102 10-11 0.05940372633333624 1102 12-13 0.07919571796123392 1102 14-15 0.06981647003700431 1102 16-17 0.05604183782666894 1102 18-19 0.008814512846036848 1102 20-21 -0.007566790678083635 1102 22-23 0.07805621238433957 1102 24-25 0.07627388442703875 1102 26-27 0.0579135677709246 1102 28-29 -0.0784556546904085 1102 30-31 0.058787554977541845 1102 32-33 -0.029989070389298433 1102 34-35 0.03119557906325099 1102 36-37 -8.215192347975631E-4 1102 38-39 -0.11063424990189219 1102 40-41 -0.2021246095858018 1102 42-43 -0.06292366717395481 1102 44-45 0.005790615101140872 1102 46-47 -0.08471123611733589 1102 48-49 -0.02786281677111191 1102 50-51 0.03037550112847498 1102 52-53 -0.015571970547938463 1102 54-55 7.263321556223445E-4 1102 56-57 0.05252254390014599 1102 58-59 0.08941735207451273 1102 60-61 -0.025327656237742247 1102 62-63 -0.1680172295318556 1102 64-65 -0.19266095537980021 1102 66-67 -0.2178116747156551 1102 68-69 -0.07577347914228483 1102 70-71 -0.02870522824806443 1102 72-73 -0.06766253733211158 1102 74-75 -0.06854496684815459 1102 76-77 -0.13822482795091773 1102 78-79 -0.062112028809707454 1102 80-81 -0.05388904466192557 1102 82-83 -0.09496179476313316 1102 84-85 -0.08599171551680129 1102 86-87 -0.15603098246706892 1102 88-89 -0.17912599909116267 1102 90-91 -0.06692412478481558 1102 92-93 -0.09257272656780557 1102 94-95 -0.16281325300272798 1102 96-97 0.03849008337057569 1102 98-99 -0.09645580272498577 1102 100-101 0.1840631284735288 1103 1 -0.02755363773824726 1103 2 0.07193275351126616 1103 3 0.030564674245248113 1103 4 0.07319799212991995 1103 5 0.11362877917669323 1103 6 0.02648473166129861 1103 7 0.04895352485163329 1103 8 0.09519959936856282 1103 9 0.13076240323231048 1103 10-11 0.09265174256602648 1103 12-13 0.037452788733538966 1103 14-15 0.09507565058036249 1103 16-17 0.09552458752662574 1103 18-19 -0.02091147742752497 1103 20-21 -0.010917820616135998 1103 22-23 0.07299750170012942 1103 24-25 0.10528195192233625 1103 26-27 -0.05671701243963412 1103 28-29 -0.07288753795867109 1103 30-31 -0.03001046890761927 1103 32-33 0.11230567016241366 1103 34-35 0.171048072381339 1103 36-37 -0.016667452036905672 1103 38-39 0.0977154648213201 1103 40-41 0.01890844373521361 1103 42-43 0.24981434239040112 1103 44-45 0.27676821954312913 1103 46-47 0.2711347401031432 1103 48-49 0.19751478738141515 1103 50-51 0.32666380924839444 1103 52-53 0.4384079564151264 1103 54-55 0.4195655636938582 1103 56-57 0.3485741133476523 1103 58-59 0.2500204983868244 1103 60-61 0.34899019591329505 1103 62-63 0.24776198370553715 1103 64-65 0.28853411359570913 1103 66-67 0.2958561392814829 1103 68-69 0.1264436800153561 1103 70-71 0.26356358791746715 1103 72-73 0.42132283682752103 1103 74-75 0.32960902870941666 1103 76-77 0.3942755601007839 1103 78-79 0.6019682416288568 1103 80-81 0.4883889798166301 1103 82-83 0.557697526238659 1103 84-85 0.4098607806958512 1103 86-87 0.4969929443121863 1103 88-89 0.3735916472567915 1103 90-91 0.3812288395864307 1103 92-93 0.4492374287722747 1103 94-95 0.36695405708802653 1103 96-97 0.39436909373562656 1103 98-99 0.4450911287358572 1103 100-101 0.4955739539399957 1104 1 0.011198869658016974 1104 2 0.001430972720232404 1104 3 0.014951599440145458 1104 4 -3.292259549070309E-4 1104 5 0.022506555302101106 1104 6 -0.04211872657526072 1104 7 -0.1347063268142179 1104 8 -0.14187027694806886 1104 9 -0.06894387075799813 1104 10-11 -0.04854062329898312 1104 12-13 -0.07684610318063534 1104 14-15 -0.03379475155562517 1104 16-17 0.007094509416710082 1104 18-19 -0.04256446178018791 1104 20-21 -0.11266288389241197 1104 22-23 -0.13280407813370942 1104 24-25 -0.06145119029947921 1104 26-27 0.001035624938040769 1104 28-29 0.0440632945734194 1104 30-31 -0.02523747835971335 1104 32-33 0.04374858490762534 1104 34-35 0.07668762090495562 1104 36-37 0.034517925899464785 1104 38-39 0.13013177815099652 1104 40-41 0.15848152447058084 1104 42-43 0.004453410965076898 1104 44-45 0.16560201672050567 1104 46-47 0.08252474396146425 1104 48-49 0.04171938680452314 1104 50-51 0.09077770037788468 1104 52-53 0.07009306148190575 1104 54-55 0.032716552980325275 1104 56-57 0.07693809054128309 1104 58-59 0.04079074447658826 1104 60-61 0.06236416385745969 1104 62-63 -0.15099254765436143 1104 64-65 -0.13054343413705283 1104 66-67 -0.10579373374106638 1104 68-69 -0.2191217265626193 1104 70-71 -0.13319153692523145 1104 72-73 -0.02397212160698814 1104 74-75 -0.027803875178118886 1104 76-77 -0.19034770739433782 1104 78-79 -0.02119748421453238 1104 80-81 0.05641665923266714 1104 82-83 0.0812184508473841 1104 84-85 0.01992408324255024 1104 86-87 4.7959860206603366E-4 1104 88-89 -0.14064086047756064 1104 90-91 -0.285118356070317 1104 92-93 -0.1466808579219432 1104 94-95 -0.14843160735570393 1104 96-97 0.03227446041456261 1104 98-99 -0.07663141508946225 1104 100-101 -0.04417580100278329 1105 1 0.010311082124538018 1105 2 0.04475974733949073 1105 3 0.0050656794571963815 1105 4 -0.027225799228020264 1105 5 -0.05001601594082672 1105 6 -0.08298138910463848 1105 7 -0.057594837012040045 1105 8 -0.024124781382198535 1105 9 -0.0185500507398757 1105 10-11 -0.024830262679863324 1105 12-13 0.03866906756282873 1105 14-15 0.08704129577488118 1105 16-17 0.06538251599279477 1105 18-19 -0.02378973035574461 1105 20-21 -0.010153788496708671 1105 22-23 -0.01389042210109892 1105 24-25 0.08851239607052719 1105 26-27 0.07263115082857041 1105 28-29 0.015156320381827015 1105 30-31 0.08178101848243102 1105 32-33 0.1446059676609508 1105 34-35 0.018979908986842986 1105 36-37 0.089112773190557 1105 38-39 0.07405695114577782 1105 40-41 0.21217897006845732 1105 42-43 0.13648606866332358 1105 44-45 0.31155830113618777 1105 46-47 0.17916463519326697 1105 48-49 0.2655173163952185 1105 50-51 0.1870581822716204 1105 52-53 0.34357664810828936 1105 54-55 0.3033938464982171 1105 56-57 0.22124710886346577 1105 58-59 0.42961812944917455 1105 60-61 0.41944923875576734 1105 62-63 0.33762520857701617 1105 64-65 0.11412451281522351 1105 66-67 0.1312976945144655 1105 68-69 0.12842213603821762 1105 70-71 0.16068600591987803 1105 72-73 0.27152616827434883 1105 74-75 0.24934607126299468 1105 76-77 0.33009544127502366 1105 78-79 0.43591579221899934 1105 80-81 0.3684903613811912 1105 82-83 0.48998732564618663 1105 84-85 0.40895344568756187 1105 86-87 0.4525058628840988 1105 88-89 0.44397976878521916 1105 90-91 0.4761455534155701 1105 92-93 0.5545140218970523 1105 94-95 0.4056431138937526 1105 96-97 0.4435085660052742 1105 98-99 0.4828533540785678 1105 100-101 0.5245265838696547 1106 1 -0.019884339944677976 1106 2 0.02984701070547402 1106 3 0.04041950687673079 1106 4 -4.0481688510851654E-4 1106 5 0.01821346238332211 1106 6 0.07444619571060684 1106 7 -0.015419625416171812 1106 8 0.013184839121088032 1106 9 -0.05076791782561685 1106 10-11 0.014396452602817078 1106 12-13 -0.00807647492536745 1106 14-15 0.06460352972403882 1106 16-17 -0.02532692089010169 1106 18-19 -0.01377573607052085 1106 20-21 0.012237897744768134 1106 22-23 -0.015140517099759165 1106 24-25 -0.01019169118672636 1106 26-27 -0.02397894759440078 1106 28-29 -0.0850203116077779 1106 30-31 -0.04679842179857019 1106 32-33 -0.008635255554885646 1106 34-35 0.1120688111342929 1106 36-37 0.10488486551090404 1106 38-39 0.09280780831758761 1106 40-41 -0.0288971834773335 1106 42-43 0.12226575214089763 1106 44-45 0.1359880689550934 1106 46-47 0.058267681876706945 1106 48-49 0.060164458617165906 1106 50-51 0.06983075417141293 1106 52-53 0.037597978473876026 1106 54-55 0.07968392828879445 1106 56-57 -0.0902643700319814 1106 58-59 0.08473473538949605 1106 60-61 0.021091558103258023 1106 62-63 -0.028717181440747197 1106 64-65 0.03054054317286159 1106 66-67 -0.08663674199716098 1106 68-69 -0.11480544231274337 1106 70-71 0.08490607784805349 1106 72-73 0.004577359229955391 1106 74-75 -0.058854387700638 1106 76-77 -0.03722580619058746 1106 78-79 -0.042708286512393556 1106 80-81 -0.030938773113323492 1106 82-83 0.0025678997962437222 1106 84-85 -0.012758923705664671 1106 86-87 0.0972287419680562 1106 88-89 0.035372409817824746 1106 90-91 0.05797429725813075 1106 92-93 0.14947564243877665 1106 94-95 0.10967931209545867 1106 96-97 0.050297672476276034 1106 98-99 -0.003371752996933708 1106 100-101 0.10564146192632151 1107 1 -0.052500321068819744 1107 2 -0.11659512294243157 1107 3 -0.051542829964489556 1107 4 -0.07366102964402188 1107 5 -0.06974638896690522 1107 6 -0.040639675678889375 1107 7 -0.03332560293716824 1107 8 0.026461146868115293 1107 9 -0.03117807180908727 1107 10-11 -0.10321660522820508 1107 12-13 -0.09920771648827298 1107 14-15 -0.028456817783464317 1107 16-17 -0.1131288000260895 1107 18-19 -0.0854519038241861 1107 20-21 -0.11148815435939952 1107 22-23 -0.07971785171691437 1107 24-25 -0.09878398487317241 1107 26-27 0.022049958415031767 1107 28-29 -0.00492055803520941 1107 30-31 -0.06108233859440304 1107 32-33 -0.1641981283116607 1107 34-35 -0.2237289194899148 1107 36-37 -0.1388071771357815 1107 38-39 -0.19199543071701441 1107 40-41 -0.0193447766449637 1107 42-43 -0.06351884257481544 1107 44-45 0.14674881332570777 1107 46-47 -0.1300763855724938 1107 48-49 -0.09347594994648745 1107 50-51 -0.03558034533919141 1107 52-53 -0.057038309067472426 1107 54-55 0.113838120608456 1107 56-57 -0.0264641664622971 1107 58-59 -0.12368213488067425 1107 60-61 -0.2631818167570046 1107 62-63 -0.09921688283866814 1107 64-65 -0.23696411573907028 1107 66-67 -0.21395612002665843 1107 68-69 -0.25218873428579514 1107 70-71 -0.3215381184326773 1107 72-73 -0.17764628809894134 1107 74-75 -0.23244823205155996 1107 76-77 -0.22505679833727044 1107 78-79 -0.24168326131524864 1107 80-81 -0.1790476173987514 1107 82-83 -0.11540644164180591 1107 84-85 -0.09857478077664794 1107 86-87 -0.13288101113036177 1107 88-89 -0.19680481904789104 1107 90-91 -0.4030640354260946 1107 92-93 -0.3641436840986927 1107 94-95 -0.2115068419928079 1107 96-97 -0.15657010150565753 1107 98-99 -0.2717273401951026 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-0.6707175175887947 2316 78-79 -0.68173240980963 2316 80-81 -0.7132510898252598 2316 82-83 -0.8005975095675417 2316 84-85 -0.8570358562551093 2316 86-87 -0.8322552920351605 2316 88-89 -0.7526089377436449 2316 90-91 -0.8702980220798331 2316 92-93 -0.840944367957885 2316 94-95 -0.8918334759901043 2316 96-97 -1.1194339364813892 2316 98-99 -0.9825525237066444 2316 100-101 -1.190620975392541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 18.0 5 59.0 6 140.0 7 361.0 8 886.0 9 1731.0 10 3118.0 11 5054.0 12 7808.0 13 11136.0 14 13085.0 15 16588.0 16 20153.0 17 25617.0 18 24154.0 19 19335.0 20 18319.0 21 18745.0 22 19753.0 23 21929.0 24 25308.0 25 30320.0 26 37505.0 27 47819.0 28 60226.0 29 75332.0 30 93781.0 31 115613.0 32 144436.0 33 183659.0 34 240775.0 35 328070.0 36 485263.0 37 684408.0 38 614483.0 39 119006.0 40 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.317407340549785 18.887397563096464 13.998756321604755 19.796438774749 2 14.452218896728478 24.933272736431082 35.891712159402104 24.722796207438336 3 27.26090014965581 24.128234274966427 29.30845465645352 19.30241091892424 4 11.909033865434662 16.127465278716468 42.777503482444075 29.1859973734048 5 11.622214222600386 40.27885164005686 34.7903205840546 13.308613553288154 6 27.007773071280898 39.245572422742384 16.771166074520746 16.975488431455968 7 26.144752999028185 34.23079604616319 21.80122394214092 17.823227012667704 8 23.260478279366716 28.515746054060052 19.97159976152906 28.252175905044176 9 20.99409371794124 23.214292320410465 25.58753349051167 30.204080471136624 10-11 25.656613228876612 24.03434209197067 27.74499829968512 22.564046379467595 12-13 31.21001145827746 22.69313771119307 25.565547024255608 20.531303806273865 14-15 23.110503004549795 25.962819051998803 30.795255213141477 20.131422730309925 16-17 19.589405971164368 29.16535170906545 29.84408325993759 21.401159059832587 18-19 18.72908932842542 33.833349221910304 28.04264588915085 19.39491556051342 20-21 22.191534114008828 26.85966354518848 25.77175437680269 25.177047964 22-23 19.676413715778494 27.91273898672616 27.697674054617817 24.71317324287753 24-25 24.989904825052538 27.031691308189544 27.016054102738856 20.962349764019063 26-27 23.450274648381345 23.809395745102282 32.94526967734738 19.795059929168993 28-29 19.303710825213486 32.36286476708299 28.915443708639426 19.417980699064096 30-31 21.329487615875266 27.10130825354072 27.12171413999861 24.447489990585403 32-33 22.20872166602587 27.532260226469734 28.871362355845314 21.38765575165908 34-35 25.65354971144178 25.274492444040707 28.709457919221915 20.362499925295594 36-37 23.729238574215707 24.45816106712238 32.11233822891716 19.70026212974476 38-39 21.1127183633775 28.199647492662738 31.550887090322675 19.136747053637084 40-41 20.760048977582297 29.93167755334067 28.379179262646907 20.929094206430122 42-43 19.27701448045795 30.3264410552339 25.436862035711126 24.95968242859702 44-45 22.320828243142383 25.306026904668222 26.482264790488095 25.8908800617013 46-47 25.343120591333506 28.69412333373697 26.853583999606133 19.109172075323393 48-49 24.022826411899032 27.447442721605398 28.154557221763977 20.375173644731596 50-51 18.142723675924074 28.43080450964562 33.11923605690302 20.30723575752729 52-53 21.678341578646542 31.78930719306526 25.580727922023133 20.95162330626507 54-55 19.408081301918916 27.318396374743063 28.16516330474588 25.108359018592136 56-57 23.738805011211177 25.040872372752055 27.964259139594756 23.256063476442016 58-59 28.20115630296105 25.374788155792118 25.760782176484902 20.66327336476193 60-61 22.701139611842127 27.096645026449785 31.97403230892349 18.2281830527846 62-63 20.26005811322688 28.061943804908037 30.299616088427726 21.37838199343736 64-65 19.29688870461033 33.212201189659844 27.27979218542473 20.211117920305092 66-67 21.944602827901438 27.138511148692984 25.191215406657076 25.725670616748502 68-69 19.85779901579658 28.636015662659347 26.817016924615515 24.68916839692855 70-71 24.907923862949893 27.526883556648475 25.949425542527354 21.615767037874274 72-73 23.257019084419678 24.62410478865592 31.59036546065367 20.528510666270734 74-75 19.716386388720792 32.52871373317589 27.62417575318903 20.13072412491429 76-77 21.503564861005422 27.296983342733768 26.173199826295907 25.026251969964903 78-79 22.255047804833627 27.850550401213418 27.9549035970834 21.939498196869547 80-81 25.308451028881535 26.24268286089112 27.318647357292665 21.13021875293468 82-83 23.113853209547138 25.12864198683216 30.910075537504667 20.847429266116034 84-85 20.875650786920374 28.388851498926442 30.254978991267823 20.48051872288536 86-87 21.125364287949303 30.025750770054316 27.134612481290244 21.714272460706134 88-89 19.536727683235746 29.97138198694354 24.865073602490348 25.626816727330365 90-91 22.011968035533023 24.91449268887234 26.54763324243098 26.52590603316366 92-93 24.49025907502476 28.433071336695086 27.041226053203722 20.035443535076435 94-95 22.86848663340682 27.28475984412969 28.32875680822733 21.51799671423616 96-97 17.545561938880024 28.14656204047819 32.78779103107339 21.520084989568403 98-99 20.937120463437488 31.366626894258758 25.7528262244601 21.943426417843654 100-101 18.49915730001315 27.015137293786108 28.213003592218588 26.27270181398216 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 411.0 1 303.5 2 479.5 3 726.0 4 565.0 5 440.0 6 603.0 7 1041.5 8 1607.0 9 1949.5 10 1947.5 11 1955.0 12 2087.5 13 2315.0 14 2624.5 15 2902.5 16 3174.5 17 3452.5 18 3772.0 19 4156.0 20 4515.0 21 5037.5 22 5814.0 23 6873.0 24 8327.5 25 10268.0 26 12900.5 27 16711.5 28 22127.5 29 28535.5 30 35567.5 31 42946.0 32 50347.5 33 57125.5 34 63153.5 35 69466.5 36 76349.0 37 83994.0 38 90852.0 39 94258.5 40 96204.5 41 97999.0 42 99906.5 43 103238.5 44 104824.0 45 107939.5 46 121905.5 47 238552.5 48 487159.0 49 469879.0 50 225724.0 51 116025.0 52 80276.0 53 64618.0 54 55490.0 55 47803.5 56 40160.0 57 32239.0 58 25389.5 59 20481.0 60 16282.0 61 12431.0 62 9265.0 63 6652.0 64 4479.0 65 2955.5 66 1921.5 67 1176.5 68 689.5 69 393.5 70 217.5 71 116.0 72 68.5 73 42.0 74 29.0 75 16.5 76 8.5 77 8.0 78 6.5 79 2.5 80 1.0 81 1.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05386939020590172 2 0.003528686944285163 3 0.037791098887183044 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.268572916473988E-5 14-15 0.001451314791601156 16-17 0.0 18-19 0.0 20-21 0.01105560385366763 22-23 0.0 24-25 0.0 26-27 0.005648744826133911 28-29 0.012606518679986512 30-31 0.010329946457867051 32-33 0.005919087777510597 34-35 0.00586217347195761 36-37 0.0204464642699104 38-39 0.042799557775845855 40-41 0.005762573437239884 42-43 0.01105560385366763 44-45 0.010031146353713872 46-47 0.0047381159372861265 48-49 0.0015793719790953755 50-51 0.002162743611013487 52-53 0.0010956003818949904 54-55 0.0011952004166127168 56-57 0.01062874656202023 58-59 0.0076834312496531785 60-61 0.006459773680263969 62-63 0.006402859374710982 64-65 0.07008996728850288 66-67 0.03221349694299036 68-69 0.024430465658619456 70-71 0.014868862325717724 72-73 2.988001041531792E-4 74-75 3.841715624826589E-4 76-77 9.390860416242774E-4 78-79 7.825717013535644E-4 80-81 0.0012805718749421965 82-83 0.0011098289582832368 84-85 0.00113828611105973 86-87 0.005108058923380539 88-89 2.703429513766859E-4 90-91 7.968002777418111E-4 92-93 4.268572916473988E-4 94-95 0.0030733724998612713 96-97 0.00603291638861657 98-99 0.008892860242654142 100-101 0.009931546318996145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3514055.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.24842480942193 #Duplication Level Percentage of deduplicated Percentage of total 1 77.31784013031545 32.66556955172537 2 13.694960901515616 11.57181051831311 3 3.84726194166957 4.876222705943322 4 1.620921379745689 2.7392550013668067 5 0.8760495257372489 1.8505856258719948 6 0.5320432985937743 1.3486794777597533 7 0.36624480147940747 1.0831286170001084 8 0.26320857920689517 0.889611829425383 9 0.20258431717637615 0.770298146261479 >10 1.1450799673427023 8.894637222959147 >50 0.08177058332806066 2.374115498351621 >100 0.04279923978553019 3.2743090961150454 >500 0.00421613078644651 1.2099486203242953 >1k 0.002944599279396942 2.68869617350438 >5k 8.030725307446206E-4 2.4394061565551386 >10k+ 0.001271531507012316 21.323725758523032 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 173166 4.92781131769423 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 76336 2.172305214346389 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 65776 1.8717976810266201 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 62597 1.7813323923501483 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 48313 1.3748504220907185 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 40464 1.151490229948023 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 36601 1.0415602487724296 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 34924 0.9938376035662505 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 31264 0.8896844244042851 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 30148 0.8579262419057185 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 28936 0.8234361727406088 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 27876 0.793271590797526 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 14846 0.42247489011981887 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 14768 0.4202552322032524 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 14701 0.41834860296722737 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 13599 0.38698882060753176 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 13559 0.38585053449647205 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 13220 0.37620355970524083 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 13093 0.3725895013026262 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 9739 0.2771442108902678 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 8474 0.24114591262800383 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7650 0.2176972187401734 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 7618 0.21678658985132562 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7580 0.21570521804581888 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 7572 0.2154775608236069 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 7549 0.21482304630974758 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6564 0.1867927508249017 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6313 0.17965000547800192 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6073 0.17282028881164352 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 5708 0.1624334280482235 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 5438 0.1547499967985703 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 4995 0.1421434781185838 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 4649 0.13229730325791714 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4356 0.12395935749440462 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 4312 0.1227072427722389 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3991 0.11357249673098457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3860 0.10984460971726397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.8457152776493254E-5 0.0 6 0.0 0.0 0.0 2.8457152776493254E-5 0.0 7 0.0 0.0 0.0 2.8457152776493254E-5 0.0 8 0.0 0.0 0.0 2.8457152776493254E-5 0.0 9 0.0 0.0 0.0 5.691430555298651E-5 0.0 10-11 0.0 0.0 0.0 1.1382861110597302E-4 0.0 12-13 0.0 2.8457152776493254E-5 0.0 2.2765722221194603E-4 0.0 14-15 0.0 4.268572916473988E-5 0.0 3.5571440970616567E-4 0.0 16-17 0.0 5.691430555298651E-5 0.0 5.406859027533718E-4 0.0 18-19 0.0 5.691430555298651E-5 0.0 8.252574305183044E-4 0.0 20-21 0.0 5.691430555298651E-5 0.0 0.0012378861457774566 0.0 22-23 0.0 5.691430555298651E-5 0.0 0.0018781720832485548 0.0 24-25 0.0 5.691430555298651E-5 0.0 0.002674972360990366 0.0 26-27 0.0 5.691430555298651E-5 0.0 0.003272572569296724 0.0 28-29 0.0 5.691430555298651E-5 0.0 0.00476657309006262 0.0 30-31 0.0 5.691430555298651E-5 0.0 0.008024917082971098 0.0 32-33 0.0 5.691430555298651E-5 0.0 0.01281994732581021 0.0 34-35 0.0 5.691430555298651E-5 0.0 0.017373091770049132 0.0 36-37 0.0 8.537145832947976E-5 0.0 0.02112943593654624 0.0 38-39 0.0 1.1382861110597302E-4 0.0 0.027617666769586702 0.0 40-41 0.0 1.1382861110597302E-4 0.0 0.04536070152573025 0.0 42-43 0.0 1.1382861110597302E-4 0.0 0.058379848920975905 0.0 44-45 0.0 1.2805718749421964E-4 0.0 0.07349059704529383 0.0 46-47 0.0 1.4228576388246626E-4 0.0 0.1140989540573497 0.0 48-49 0.0 1.4228576388246626E-4 0.0 0.12992113100107994 0.0 50-51 0.0 1.4228576388246626E-4 0.0 0.14911548054882465 0.0 52-53 0.0 1.4228576388246626E-4 0.0 0.17033028794370036 0.0 54-55 0.0 1.4228576388246626E-4 0.0 0.19996841256041809 0.0 56-57 0.0 1.4228576388246626E-4 0.0 0.33966457554022345 0.0 58-59 0.0 1.4228576388246626E-4 0.0 0.42980260695976585 0.0 60-61 0.0 1.4228576388246626E-4 0.0 0.47832205244368686 0.0 62-63 0.0 1.4228576388246626E-4 0.0 0.5422795033088554 0.0 64-65 0.0 1.4228576388246626E-4 0.0 0.6029359244519508 0.0 66-67 0.0 1.4228576388246626E-4 0.0 0.7774067281246309 0.0 68-69 0.0 1.4228576388246626E-4 0.0 0.9564022190887735 0.0 70-71 0.0 1.4228576388246626E-4 0.0 0.9985045766215952 0.0 72-73 0.0 1.4228576388246626E-4 0.0 1.0308888164812446 0.0 74-75 0.0 1.4228576388246626E-4 0.0 1.1668570924473294 0.0 76-77 0.0 1.4228576388246626E-4 0.0 1.2978880524066927 2.8457152776493254E-5 78-79 0.0 1.4228576388246626E-4 0.0 1.407305804832309 2.8457152776493254E-5 80-81 0.0 1.4228576388246626E-4 0.0 1.4993646940642649 2.8457152776493254E-5 82-83 0.0 1.4228576388246626E-4 0.0 1.720974771311206 2.8457152776493254E-5 84-85 0.0 1.4228576388246626E-4 0.0 2.3111192055901233 2.8457152776493254E-5 86-87 0.0 1.4228576388246626E-4 0.0 2.8312305868860905 2.8457152776493254E-5 88-89 0.0 1.7074291665895953E-4 0.0 3.475528982898674 2.8457152776493254E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 505 0.0 45.15499 2 TACCTGG 3010 0.0 43.87672 2 ACCTGGG 3295 0.0 38.784016 3 ATAACGC 645 0.0 36.82699 3 GTACCTG 3610 0.0 36.74142 1 TAACGCA 635 0.0 36.642105 4 TAGACCG 100 1.3033768E-6 33.239628 5 TACGCAG 2670 0.0 32.546024 4 AGTACGC 2690 0.0 32.142155 2 GTACGCA 2685 0.0 32.025074 3 GAGTACG 2805 0.0 31.52383 1 CGCAGTG 2975 0.0 29.5286 6 ACGCAGT 2990 0.0 29.380463 5 GTATAAC 945 0.0 29.17797 1 GTACCGG 250 0.0 26.622385 1 CCTGGGG 2605 0.0 25.155296 4 GTACACG 310 0.0 24.536758 1 CTAACGC 175 4.048132E-6 21.717402 3 GTACCCG 330 1.8189894E-12 21.609077 1 ACCGTCC 210 8.643892E-7 20.350792 8 >>END_MODULE