##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003825_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3429418 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.732011962379623 31.0 31.0 34.0 30.0 34.0 2 31.93476648224276 31.0 31.0 34.0 30.0 34.0 3 31.99145569306512 33.0 31.0 34.0 30.0 34.0 4 35.73748082036077 37.0 35.0 37.0 35.0 37.0 5 35.601580501414524 37.0 35.0 37.0 33.0 37.0 6 35.67912018890669 37.0 35.0 37.0 33.0 37.0 7 35.631903139249864 37.0 35.0 37.0 33.0 37.0 8 35.63239418466924 37.0 35.0 37.0 33.0 37.0 9 37.22691576238301 39.0 37.0 39.0 33.0 39.0 10-11 37.13002978931119 39.0 37.0 39.0 33.0 39.0 12-13 37.09999524700693 39.0 37.0 39.0 33.0 39.0 14-15 38.21619280589301 40.0 37.5 41.0 33.0 41.0 16-17 38.23253070929236 40.0 38.0 41.0 33.0 41.0 18-19 38.26215701906271 40.0 38.0 41.0 33.5 41.0 20-21 38.205446959221646 40.0 37.5 41.0 33.0 41.0 22-23 38.12343785446977 40.0 37.0 41.0 33.0 41.0 24-25 38.00536140534633 40.0 37.0 41.0 33.0 41.0 26-27 37.8065076931421 40.0 37.0 41.0 32.5 41.0 28-29 37.65341291146194 39.0 37.0 41.0 32.0 41.0 30-31 37.487903924222714 39.0 36.5 41.0 32.0 41.0 32-33 37.24587072791943 39.0 36.0 40.0 31.5 41.0 34-35 37.22147081516455 39.0 36.0 41.0 31.0 41.0 36-37 37.074688329040086 39.0 36.0 40.5 31.0 41.0 38-39 36.848013569649424 39.0 35.5 40.0 30.5 41.0 40-41 36.891368593738065 39.0 36.0 41.0 30.5 41.0 42-43 36.77514537452127 39.0 35.5 41.0 30.0 41.0 44-45 36.562970159951334 39.0 35.0 41.0 30.0 41.0 46-47 36.39705891786886 39.0 35.0 41.0 30.0 41.0 48-49 36.168786802891915 39.0 35.0 40.0 29.5 41.0 50-51 35.93837700741059 39.0 35.0 40.0 28.5 41.0 52-53 35.7593746227494 39.0 35.0 40.0 28.0 41.0 54-55 35.58702409563372 38.5 35.0 40.0 28.0 41.0 56-57 35.31636155172686 38.0 34.0 40.0 27.5 41.0 58-59 35.05827446522996 38.0 34.0 40.0 26.5 41.0 60-61 34.67958513660335 38.0 34.0 40.0 26.0 41.0 62-63 34.45434356500141 37.5 34.0 40.0 26.0 41.0 64-65 34.001607269804964 37.0 33.0 39.5 25.5 41.0 66-67 33.63711918465465 36.0 33.0 39.0 24.5 41.0 68-69 33.33142416584972 36.0 32.0 39.0 24.0 40.0 70-71 32.8961054033075 35.5 32.0 38.5 23.0 40.0 72-73 32.48322805793869 35.0 31.5 38.0 22.5 40.0 74-75 32.10966729631675 35.0 31.0 37.0 22.0 39.5 76-77 31.128831626824145 34.0 30.5 36.0 20.5 39.0 78-79 31.571359630117996 34.5 31.0 36.0 21.5 39.0 80-81 31.36275353427316 35.0 31.0 36.0 20.0 38.5 82-83 31.045819144822822 34.0 31.0 35.5 20.0 37.0 84-85 30.70625773819348 34.0 31.0 35.0 19.5 37.0 86-87 30.431007389592054 34.0 31.0 35.0 18.0 36.5 88-89 30.087828313725538 34.0 30.5 35.0 16.5 36.0 90-91 29.77018797358619 34.0 30.0 35.0 10.5 36.0 92-93 29.513394692627145 34.0 30.0 35.0 7.0 35.5 94-95 29.164439126405703 34.0 29.0 35.0 2.0 35.0 96-97 28.786103210515606 34.0 29.0 35.0 2.0 35.0 98-99 28.43471880651469 34.0 29.0 35.0 2.0 35.0 100-101 27.475321614338057 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.028160701587058412 1101 2 0.073815237723867 1101 3 0.013061279680595561 1101 4 0.037957208497559236 1101 5 0.02212519679370928 1101 6 0.023645555355336967 1101 7 -0.05129662011200509 1101 8 -0.11304386685946355 1101 9 -0.0038773612998070917 1101 10-11 0.044453895807357924 1101 12-13 0.06940021983467659 1101 14-15 0.10129646017592364 1101 16-17 0.13845257584407733 1101 18-19 -0.020797920789021873 1101 20-21 -0.05379645796475785 1101 22-23 -0.11567058588524048 1101 24-25 -0.10190599194827854 1101 26-27 -0.026529195449924714 1101 28-29 0.04100685828469608 1101 30-31 0.04007276607674015 1101 32-33 0.06637247596540163 1101 34-35 0.09769642194026318 1101 36-37 0.04464458464053678 1101 38-39 -0.02463906325753129 1101 40-41 0.0986045129796409 1101 42-43 0.009673198647185188 1101 44-45 0.13523636254083016 1101 46-47 0.013811149106317089 1101 48-49 0.05691333296969958 1101 50-51 0.01743363916369134 1101 52-53 0.03215964034765051 1101 54-55 0.02114540605821702 1101 56-57 0.025737545285899444 1101 58-59 -0.13325700266766916 1101 60-61 -0.18966671759825715 1101 62-63 -0.3283396216879382 1101 64-65 -0.39793743668958825 1101 66-67 -0.39108240843747666 1101 68-69 -0.329890686948076 1101 70-71 -0.2755828716757165 1101 72-73 -0.282203823273683 1101 74-75 -0.37251751159185886 1101 76-77 -0.23639544226849551 1101 78-79 -0.2522858534239312 1101 80-81 -0.3161906321247052 1101 82-83 -0.24874379259032509 1101 84-85 -0.35796928717198284 1101 86-87 -0.5432078942235883 1101 88-89 -0.5454355900924703 1101 90-91 -0.4987862304322661 1101 92-93 -0.40654309272966316 1101 94-95 -0.4073446754722667 1101 96-97 -0.45460802702561054 1101 98-99 -0.5331971499410741 1101 100-101 -0.4060915124010869 1102 1 -0.07691446300313487 1102 2 0.015104032635658626 1102 3 -0.022848239509844603 1102 4 -0.0076171179971638026 1102 5 0.08880169519545689 1102 6 -0.09152243664377835 1102 7 -0.09434096196500263 1102 8 -0.10518502887394732 1102 9 -0.0786182643149047 1102 10-11 0.037732125110544246 1102 12-13 -0.0713548957937391 1102 14-15 -0.0599517692351057 1102 16-17 -0.10531470950072475 1102 18-19 -0.14640703680554168 1102 20-21 -0.11773816596473097 1102 22-23 -0.07872370616765068 1102 24-25 -0.08823849077505486 1102 26-27 -0.20262794312108667 1102 28-29 -0.244203605354393 1102 30-31 -0.2757891571945734 1102 32-33 -0.11503233209710118 1102 34-35 0.010340066335530196 1102 36-37 -0.10360097663873091 1102 38-39 -0.1767272384391987 1102 40-41 -0.0910027144968737 1102 42-43 0.06359632890225697 1102 44-45 0.24801456805862898 1102 46-47 0.1400917144059406 1102 48-49 0.08958056294317629 1102 50-51 0.08877280153823364 1102 52-53 0.10014304496105808 1102 54-55 0.019307414129905 1102 56-57 0.0783163544828227 1102 58-59 0.005120566526478854 1102 60-61 -0.0625687818060996 1102 62-63 -0.18380391942251606 1102 64-65 -0.307192142871898 1102 66-67 -0.2771191678790714 1102 68-69 -0.19122577437939725 1102 70-71 -0.20231451248180576 1102 72-73 -0.13737525307195142 1102 74-75 -0.24526591358755567 1102 76-77 -0.20149946513528505 1102 78-79 -0.14660799994973317 1102 80-81 -0.17927181188792218 1102 82-83 -0.07016170478102168 1102 84-85 -0.03402440072951762 1102 86-87 -0.07421226418503224 1102 88-89 -0.1592304142198664 1102 90-91 -0.13205314993199124 1102 92-93 -0.10262133534472895 1102 94-95 -0.12797817267005485 1102 96-97 -0.12031464394872415 1102 98-99 -0.20718654053295182 1102 100-101 0.012572713401713287 1103 1 -0.1217018996131749 1103 2 -0.10208890134313009 1103 3 -0.10032415442426412 1103 4 -0.04256335055661964 1103 5 -0.05683287750793653 1103 6 -0.06020153420807617 1103 7 -0.04877458384111577 1103 8 -0.016392639743280313 1103 9 -0.032810282537909075 1103 10-11 -0.06279947377779393 1103 12-13 -0.07802096022255967 1103 14-15 -0.03665784705486175 1103 16-17 -0.050792310681110564 1103 18-19 -0.30893523127877387 1103 20-21 -0.16347784688495182 1103 22-23 -0.18022653757117268 1103 24-25 -0.09993076140657564 1103 26-27 -0.2350699644438521 1103 28-29 -0.17965741813990377 1103 30-31 -0.23560430560191747 1103 32-33 -0.16706444200196557 1103 34-35 -0.22559245468522704 1103 36-37 -0.4011721159841741 1103 38-39 -0.1605601280649438 1103 40-41 -0.18769260274632416 1103 42-43 -0.09258842349827745 1103 44-45 -0.06812526448381817 1103 46-47 -0.10619161015951306 1103 48-49 -0.1344841174013709 1103 50-51 -0.022174040344218326 1103 52-53 -0.005349718442715812 1103 54-55 0.025899049046238076 1103 56-57 -0.163623028506791 1103 58-59 -0.160625420178242 1103 60-61 -0.12455053399940397 1103 62-63 -0.20727319909291708 1103 64-65 -0.14336541732761532 1103 66-67 -0.06337103437356717 1103 68-69 -0.07642765690405184 1103 70-71 -0.19340025875191458 1103 72-73 0.011250457878681175 1103 74-75 -0.13277890155519856 1103 76-77 -0.1785399975135391 1103 78-79 -0.044654800915438386 1103 80-81 -0.06706399023534715 1103 82-83 -0.03214923137247894 1103 84-85 -0.15782328162989856 1103 86-87 -0.03670258852553232 1103 88-89 -0.23064969397882962 1103 90-91 -0.1626014874898445 1103 92-93 -0.2249112561858766 1103 94-95 -0.11688937978496483 1103 96-97 -0.0806317398869858 1103 98-99 -0.13466948745985619 1103 100-101 0.035250971375607065 1104 1 -0.009838961055212536 1104 2 -4.261954374982224E-4 1104 3 0.027304844634400638 1104 4 0.013480487208141767 1104 5 -0.023136017204748782 1104 6 -0.018037756585236764 1104 7 0.010326135383770918 1104 8 -0.10080940920479975 1104 9 -0.030371686663144715 1104 10-11 -0.0100635526650521 1104 12-13 0.06268265587520716 1104 14-15 -0.01781215768826172 1104 16-17 -0.06481048271438539 1104 18-19 -0.09599199437840866 1104 20-21 -0.06771082470530843 1104 22-23 -0.029121919847796107 1104 24-25 0.023256612488658845 1104 26-27 -0.012593863090287982 1104 28-29 -0.022148943344205918 1104 30-31 -0.04026382502136272 1104 32-33 0.02225093505939668 1104 34-35 0.0956606499657866 1104 36-37 0.10048245263809008 1104 38-39 0.13205099671105103 1104 40-41 0.11851104016749048 1104 42-43 0.055819942368778186 1104 44-45 0.24448682197111538 1104 46-47 0.2591290635335781 1104 48-49 0.19653775894521175 1104 50-51 0.1551981349550644 1104 52-53 0.10561249682171336 1104 54-55 0.07128718821597602 1104 56-57 0.14023134371104362 1104 58-59 0.06835851053649122 1104 60-61 0.1054650932834349 1104 62-63 -0.09591680642460432 1104 64-65 -0.024638717236442176 1104 66-67 0.07636099009006614 1104 68-69 0.04514994041509368 1104 70-71 0.0452782094414772 1104 72-73 0.1685153320004531 1104 74-75 0.09981132519760649 1104 76-77 -0.007352305524509717 1104 78-79 0.11611979448588627 1104 80-81 0.04790794538394394 1104 82-83 0.14036217469460865 1104 84-85 0.08883945201647592 1104 86-87 0.2017752956328387 1104 88-89 0.1014909848555412 1104 90-91 -0.06302074252155165 1104 92-93 0.008201236208535079 1104 94-95 0.13532146462463146 1104 96-97 0.2414370904340153 1104 98-99 0.22228853710364405 1104 100-101 0.21440277054745494 1105 1 -0.058295640937558346 1105 2 -0.029608242510647642 1105 3 -0.09420013410172245 1105 4 -0.06902334904232532 1105 5 -0.07298180595386583 1105 6 -0.018850321902732503 1105 7 -0.03509550064643463 1105 8 0.03450232485534599 1105 9 -0.014804213730919003 1105 10-11 -0.08063924251858623 1105 12-13 -0.06300192482507327 1105 14-15 -0.007505674534563411 1105 16-17 0.042853186345638505 1105 18-19 8.186189968029112E-4 1105 20-21 -0.06900671254610558 1105 22-23 -0.03409754727232439 1105 24-25 -0.12309453959807826 1105 26-27 -0.013984645870316115 1105 28-29 -0.13134260896951844 1105 30-31 -0.08917230206080973 1105 32-33 0.02530742094317162 1105 34-35 -0.021152868047465745 1105 36-37 -0.050665935546852836 1105 38-39 -0.014493611921260197 1105 40-41 0.096269555543536 1105 42-43 0.10749420235763552 1105 44-45 0.11473367555225167 1105 46-47 0.07859245796021952 1105 48-49 0.008988692363075756 1105 50-51 0.10423979172106357 1105 52-53 0.22873674749644124 1105 54-55 0.07167342377079677 1105 56-57 -0.08996735855981086 1105 58-59 0.04656698973608542 1105 60-61 0.06023986307452134 1105 62-63 0.05386395478026884 1105 64-65 -0.21387373330162518 1105 66-67 -0.14774255563283134 1105 68-69 -0.15108605839659361 1105 70-71 -0.09376547435375215 1105 72-73 -0.17638389164655166 1105 74-75 -0.04034343844465127 1105 76-77 -0.052580724139605906 1105 78-79 0.022385714098355436 1105 80-81 0.021839217534900257 1105 82-83 0.12490458221070355 1105 84-85 0.0743061784870136 1105 86-87 0.14898620941968943 1105 88-89 0.22581160524286403 1105 90-91 0.08047248167688537 1105 92-93 0.010988979305576407 1105 94-95 0.03372051007958632 1105 96-97 0.1342858381037857 1105 98-99 0.1726218172171663 1105 100-101 0.3321606944741582 1106 1 -0.07748034662569836 1106 2 4.8152504943033136E-4 1106 3 0.028439495243059554 1106 4 -0.02175851883792035 1106 5 0.03197558378726484 1106 6 -0.00943062125384131 1106 7 -0.055775367217819394 1106 8 -0.03608569662800676 1106 9 -0.1258444307345954 1106 10-11 -0.08318367974592888 1106 12-13 -0.15668583001460235 1106 14-15 -0.0881929140139377 1106 16-17 -0.1575600653250504 1106 18-19 -0.12784325789290563 1106 20-21 -0.07181177619089141 1106 22-23 0.007778538875236052 1106 24-25 -0.004469385598646625 1106 26-27 0.010155881613322038 1106 28-29 -0.025787929939127707 1106 30-31 -0.046812379962766215 1106 32-33 0.06330907779845063 1106 34-35 0.09353723239814826 1106 36-37 -0.010737620952099292 1106 38-39 0.10972269366208565 1106 40-41 0.21720943382641167 1106 42-43 0.3093414115792186 1106 44-45 0.3352993938998594 1106 46-47 0.21048497101092067 1106 48-49 0.28602927337959017 1106 50-51 0.28369731638569107 1106 52-53 0.25211209969400983 1106 54-55 0.30213185454764613 1106 56-57 0.22017558373827484 1106 58-59 0.15172291168411078 1106 60-61 -0.005641515245905282 1106 62-63 -0.09620046907676993 1106 64-65 0.030075756042535318 1106 66-67 0.049007805774174074 1106 68-69 -0.15985711062797492 1106 70-71 0.037432910947323705 1106 72-73 0.1160215320555622 1106 74-75 0.02459209449071409 1106 76-77 -0.015197604018663213 1106 78-79 0.05027764059484596 1106 80-81 0.02213516727297815 1106 82-83 0.016588002584320805 1106 84-85 0.03809625586780285 1106 86-87 0.14575597415390362 1106 88-89 0.060554411824565335 1106 90-91 0.13498389333664562 1106 92-93 0.08517107071163821 1106 94-95 0.14746222613728222 1106 96-97 0.18194897995146064 1106 98-99 0.12232581848078539 1106 100-101 0.0821430328138355 1107 1 -0.10403273791508028 1107 2 -0.03375459578672135 1107 3 0.008359496467825522 1107 4 -0.05087108883825664 1107 5 -0.0979229135518267 1107 6 6.449753064003971E-4 1107 7 -0.008383320340008993 1107 8 0.04516254875542103 1107 9 0.09669105912993103 1107 10-11 -0.057761475206454804 1107 12-13 -0.03495782303266992 1107 14-15 -0.054792857114811966 1107 16-17 -0.0446067613955492 1107 18-19 -0.09319405957599258 1107 20-21 -0.10391897007892226 1107 22-23 -0.1001717655933021 1107 24-25 0.05030226511362912 1107 26-27 0.006607294337371172 1107 28-29 0.055042635690554675 1107 30-31 -0.023229440945605973 1107 32-33 -0.04100538405541698 1107 34-35 -0.04872808901473036 1107 36-37 -0.0055517046212401056 1107 38-39 0.07807351985930922 1107 40-41 0.2516626598460121 1107 42-43 0.188658355373164 1107 44-45 0.23687527791431506 1107 46-47 0.14230873063026195 1107 48-49 0.23533824209192034 1107 50-51 0.04510350989924916 1107 52-53 0.08631986489317711 1107 54-55 0.07261758125547857 1107 56-57 0.19496137376022915 1107 58-59 0.22202197395690604 1107 60-61 -0.09145446403208979 1107 62-63 0.059001794715562994 1107 64-65 0.02560187708813544 1107 66-67 0.04922373690839521 1107 68-69 -0.1414262753238873 1107 70-71 -0.0881464471257658 1107 72-73 0.00899985356528532 1107 74-75 -0.06900123157478433 1107 76-77 -0.05178609685222213 1107 78-79 0.050067767554651255 1107 80-81 0.055550205040265865 1107 82-83 0.08533633367333948 1107 84-85 0.14667129541212987 1107 86-87 0.1559265278157973 1107 88-89 -0.0224784353490719 1107 90-91 -0.006415590250753667 1107 92-93 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-0.570122545487159 2316 64-65 -0.4877029478940784 2316 66-67 -0.3846865090484428 2316 68-69 -0.16341857224036005 2316 70-71 -0.31930870109960807 2316 72-73 -0.6865340563968765 2316 74-75 -0.5455771317032116 2316 76-77 -0.055277109157376714 2316 78-79 -0.09355464862361274 2316 80-81 -0.26890436726570144 2316 82-83 -0.32388910540743865 2316 84-85 -0.3760607800084905 2316 86-87 -0.44215795068702235 2316 88-89 -0.23998455823733167 2316 90-91 -0.3208483059358933 2316 92-93 -0.3771790111545812 2316 94-95 -0.5862888119334286 2316 96-97 -0.8795016096462511 2316 98-99 -0.8019269855502031 2316 100-101 -1.123435091280225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 14.0 5 54.0 6 119.0 7 348.0 8 837.0 9 1631.0 10 2798.0 11 4804.0 12 7395.0 13 10563.0 14 13122.0 15 17285.0 16 21645.0 17 27305.0 18 24877.0 19 17892.0 20 16459.0 21 16668.0 22 17870.0 23 19577.0 24 22976.0 25 27952.0 26 35432.0 27 44530.0 28 56402.0 29 71371.0 30 89828.0 31 112081.0 32 141321.0 33 181009.0 34 239116.0 35 325378.0 36 474752.0 37 664317.0 38 605382.0 39 116246.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.96291423186774 20.719951688143464 14.72385514611711 20.593278933871687 2 15.031114221561252 23.16776018429417 38.980491645525326 22.82063394861925 3 22.268366198884006 26.069356759706885 29.84873222041335 21.81354482099576 4 12.597472807339322 17.18883495683524 40.448758360748094 29.764933875077343 5 11.993201178742282 39.9522601211051 35.002615604163736 13.051923095988883 6 28.195950449901414 39.85947469803914 17.095670460702078 14.848904391357367 7 26.83846063676111 33.28276109823883 22.53568972927768 17.343088535722387 8 24.185444877235728 33.84448906490839 19.67642906172418 22.293636996131706 9 23.46541016580656 20.230692204916405 23.187287172342362 33.11661045693467 10-11 24.718975639598323 26.324583354959934 28.32215262181513 20.634288383626608 12-13 27.789442376146972 23.9244273991137 27.699558306913634 20.586571917825694 14-15 22.287224519051044 26.815102266716355 28.62094626237036 22.276726951862237 16-17 20.07533056629434 29.809562438874472 28.92333334694108 21.19177364789011 18-19 19.637428275001763 31.45048226841989 29.47549700853031 19.43659244804804 20-21 21.823532096440164 27.845187958117247 28.38450666416646 21.946773281276133 22-23 20.458136045241496 28.231743695285903 28.978021926752582 22.33209833272001 24-25 22.65089878224235 28.178979640277156 28.90843285945312 20.261688718027372 26-27 22.10637265143994 26.49575104567157 31.480812672021667 19.917063630866817 28-29 20.101445761032036 30.264277785125188 29.87877456110331 19.755501892739467 30-31 21.252697680312068 28.100755780628962 29.00421349195205 21.642333047106916 32-33 21.428997385871927 28.50605401967573 29.549489020997445 20.5154595734549 34-35 22.883698808677277 27.103361064814212 29.767423278901205 20.245516847607302 36-37 22.33480641955469 26.84831246440933 31.092492364183077 19.724388751852903 38-39 21.32708369190736 28.498846242484532 30.775686784735978 19.39838328087213 40-41 20.800746635670574 28.961949254494314 29.671084008323106 20.566220101512005 42-43 20.135455966749596 29.447893284681793 28.262624412695086 22.15402633587352 44-45 21.62459660744896 27.663907295390374 28.271540564390868 22.439955532769794 46-47 22.785146584743874 28.935027972629545 28.297234522467775 19.98259092015881 48-49 22.468758884636415 28.4241589210862 28.702664479679243 20.404417714598143 50-51 20.256130207784302 29.110389594294777 30.38324032006653 20.250239877854387 52-53 21.33338562651066 30.209472082879046 27.79823659241327 20.65890569819702 54-55 20.275595286781723 28.752690175604805 28.58735399860587 22.3843605390076 56-57 22.38573797238169 27.806366503756863 28.39209231513562 21.415803208725826 58-59 24.00394447407644 27.429390385410052 27.770820981942496 20.795844158571008 60-61 21.655556613447573 28.47391041456131 30.285821847712246 19.58471112427887 62-63 21.072703697218035 28.39409094825971 29.47747347897703 21.055731875545224 64-65 20.419531833940983 30.690393274254628 28.378100971677796 20.511973920126593 66-67 21.52017590326021 28.022374694012804 27.789704828822188 22.667744573904805 68-69 20.56771285877996 29.28602908127655 28.030986466790857 22.115271593152634 70-71 22.571877115308734 28.685955997787026 27.63660246384162 21.105564423062624 72-73 21.88020679942031 27.179879337845286 29.844608839421362 21.09530502331305 74-75 20.469694544146073 30.768569069974067 28.151740290354084 20.609996095525776 76-77 21.4197714282715 27.889506654695612 27.92551896361532 22.765202953417567 78-79 21.45612739780672 28.663646299160433 28.64065383170855 21.239572471324298 80-81 22.56525392987404 28.280994304496566 28.201664911243224 20.952086854386167 82-83 21.500784617158878 27.65908526600803 29.750644394999153 21.089485721833938 84-85 20.730250745763797 28.92793720129353 28.99204518614206 21.349766866800607 86-87 21.09571176910921 29.26734423512265 27.88278860838155 21.75415538738659 88-89 20.69030522884327 28.960035551299562 27.329624143857963 23.02003507599921 90-91 21.614181551502675 26.988672896160683 28.043205997576827 23.35393955475982 92-93 22.24516110972117 28.88541500821426 28.104214670662696 20.76520921140187 94-95 21.50780559831208 28.578356262929926 28.35940496920437 21.554433169553626 96-97 19.41091267301203 29.02012120137561 29.781238544965266 21.78772758064709 98-99 20.927379744751633 30.015267892384905 27.172036675020188 21.885315687843267 100-101 20.038427816174647 28.913279031235252 27.599363842769954 23.448929309820148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 249.0 1 192.0 2 269.5 3 413.5 4 344.5 5 303.0 6 473.5 7 900.0 8 1447.5 9 1774.5 10 1832.0 11 1883.0 12 2053.5 13 2385.5 14 2738.5 15 3081.5 16 3451.5 17 3825.0 18 4336.0 19 4910.0 20 5472.0 21 6194.0 22 7371.5 23 9147.0 24 11524.0 25 14361.0 26 18246.5 27 23574.0 28 30453.5 29 38734.5 30 47455.0 31 56146.0 32 64860.0 33 73449.0 34 81532.0 35 89640.0 36 96803.0 37 104268.5 38 111494.0 39 115011.5 40 116847.0 41 118761.0 42 121224.0 43 125412.0 44 127088.0 45 128127.0 46 135318.0 47 180853.0 48 293640.0 49 284269.5 50 167678.0 51 116901.5 52 91320.0 53 75517.0 54 64285.0 55 54505.5 56 44655.0 57 35114.0 58 27491.5 59 21850.5 60 17161.5 61 12927.0 62 9305.5 63 6384.0 64 4072.0 65 2527.5 66 1563.0 67 944.0 68 536.0 69 281.0 70 153.0 71 78.0 72 47.0 73 35.0 74 22.0 75 13.5 76 10.0 77 7.0 78 6.0 79 4.5 80 1.0 81 1.0 82 1.5 83 1.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0492211798036868 2 0.003440817071584741 3 0.03671176858580669 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.4579733354172631E-5 14-15 0.0015162922688339538 16-17 0.0 18-19 0.0 20-21 0.010774422948733575 22-23 0.0 24-25 0.0 26-27 0.004738413340106106 28-29 0.011474250149733863 30-31 0.009170652279774585 32-33 0.004884210673647832 34-35 0.004738413340106106 36-37 0.019697219761487226 38-39 0.043389286462017757 40-41 0.004913370140356177 42-43 0.011867902950296522 44-45 0.01096395948233782 46-47 0.005044587740543731 48-49 0.0012538570684588464 50-51 0.0019974234695216506 52-53 0.0010205813347920843 54-55 0.0012101178683963285 56-57 0.012378193617692565 58-59 0.00924355094654545 60-61 0.004855051206939486 62-63 0.005963110941856606 64-65 0.07020141610034122 66-67 0.03411657604876396 68-69 0.02592276590371894 70-71 0.01589190935604817 72-73 2.332757336667621E-4 74-75 5.540298674585601E-4 76-77 0.0010205813347920843 78-79 9.91421868083739E-4 80-81 0.0013413354685838821 82-83 0.0011226394682712925 84-85 0.0013996544020005728 86-87 0.005686096008127327 88-89 2.1869600031258947E-4 90-91 9.622624013753937E-4 92-93 4.665514673335242E-4 94-95 0.003397077871522223 96-97 0.007260707210377971 98-99 0.00952056588027473 100-101 0.011415931216317171 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3429418.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.040425508773005 #Duplication Level Percentage of deduplicated Percentage of total 1 78.06679061179351 39.84562210930226 2 13.68715221519245 13.971961461335352 3 3.6211820420014056 5.544800168054378 4 1.510466801181876 3.083794729967927 5 0.7690285602653473 1.9625772472171206 6 0.4789233241337534 1.466667014991767 7 0.3026437488347271 1.0812946002666286 8 0.22601437409127864 0.9228695859774291 9 0.16943450094011858 0.7783208121465227 >10 1.0173226895160326 9.855946146221473 >50 0.0959099322954484 3.3504180039099163 >100 0.04864286702631227 4.604731960573686 >500 0.0026986870639439785 0.9435043251374169 >1k 0.00246901156880003 2.6253064992425807 >5k 6.316076106232636E-4 2.335725638347114 >10k+ 6.890264843162875E-4 7.626459697308532 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 77667 2.2647283008370516 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 29988 0.8744340876498579 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 20638 0.6017930739268296 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 19902 0.5803317064294875 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17241 0.5027383655185808 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15912 0.46398543426318983 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 14724 0.42934398781367566 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14649 0.42715702781054976 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14548 0.42421192167300686 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 12062 0.3517214874360606 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11468 0.3344007642113035 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11429 0.33326354500967803 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9834 0.2867541956098673 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9653 0.2814763321356568 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9012 0.26278511397560755 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7678 0.22388638538667494 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6848 0.19968402801874835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6730 0.19624321094716363 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 6474 0.18877838746982725 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6089 0.1775519927871143 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6009 0.17521923545044668 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5917 0.17253656451327895 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5455 0.15906489089402343 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4824 0.14066526740105756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4693 0.13684537726226434 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4395 0.12815585618317743 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4085 0.1191164215035904 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3462 0.10095007374429131 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.831893341669053E-5 0.0 10-11 0.0 0.0 0.0 5.831893341669053E-5 0.0 12-13 0.0 0.0 0.0 5.831893341669053E-5 0.0 14-15 0.0 0.0 0.0 1.1663786683338106E-4 0.0 16-17 0.0 0.0 0.0 3.499136005001432E-4 0.0 18-19 0.0 0.0 0.0 5.394501341043874E-4 0.0 20-21 0.0 0.0 0.0 8.018853344794948E-4 0.0 22-23 0.0 0.0 0.0 0.0011226394682712928 0.0 24-25 0.0 0.0 0.0 0.0016183504023131621 0.0 26-27 0.0 0.0 0.0 0.0022744384032509306 0.0 28-29 0.0 0.0 0.0 0.0046071957399185515 0.0 30-31 2.9159466708345266E-5 0.0 0.0 0.0098558997474207 0.0 32-33 2.9159466708345266E-5 0.0 0.0 0.020032553628633196 0.0 34-35 2.9159466708345266E-5 0.0 0.0 0.030384164310095767 0.0 36-37 2.9159466708345266E-5 1.4579733354172633E-5 0.0 0.04315601072835099 0.0 38-39 2.9159466708345266E-5 2.9159466708345266E-5 0.0 0.06785407903031943 0.0 40-41 2.9159466708345266E-5 2.9159466708345266E-5 0.0 0.096109602270706 0.0 42-43 2.9159466708345266E-5 2.9159466708345266E-5 0.0 0.12522532977898876 0.0 44-45 2.9159466708345266E-5 2.9159466708345266E-5 0.0 0.1464388418093099 0.0 46-47 2.9159466708345266E-5 2.9159466708345266E-5 0.0 0.1781935010546979 0.0 48-49 2.9159466708345266E-5 2.9159466708345266E-5 0.0 0.20232295975585363 0.0 50-51 2.9159466708345266E-5 2.9159466708345266E-5 0.0 0.23021398966238588 0.0 52-53 5.831893341669053E-5 5.831893341669053E-5 0.0 0.29550203562237093 0.0 54-55 5.831893341669053E-5 5.831893341669053E-5 0.0 0.3826159424135524 2.9159466708345266E-5 56-57 7.289866677086316E-5 5.831893341669053E-5 0.0 0.5151019794029191 2.9159466708345266E-5 58-59 8.74784001250358E-5 5.831893341669053E-5 0.0 0.6955990783275763 2.9159466708345266E-5 60-61 8.74784001250358E-5 5.831893341669053E-5 0.0 0.9028645676904945 2.9159466708345266E-5 62-63 8.74784001250358E-5 5.831893341669053E-5 0.0 1.0235556003963355 2.9159466708345266E-5 64-65 8.74784001250358E-5 5.831893341669053E-5 0.0 1.1824309547567546 2.9159466708345266E-5 66-67 8.74784001250358E-5 5.831893341669053E-5 0.0 1.4437143561968824 2.9159466708345266E-5 68-69 8.74784001250358E-5 5.831893341669053E-5 0.0 1.745864750228756 2.9159466708345266E-5 70-71 8.74784001250358E-5 5.831893341669053E-5 0.0 2.0546780824034867 2.9159466708345266E-5 72-73 1.1663786683338106E-4 5.831893341669053E-5 0.0 2.368186088718261 2.9159466708345266E-5 74-75 1.749568002500716E-4 5.831893341669053E-5 0.0 2.6517619024569186 2.9159466708345266E-5 76-77 1.749568002500716E-4 5.831893341669053E-5 0.0 2.8894261358632862 2.9159466708345266E-5 78-79 1.749568002500716E-4 5.831893341669053E-5 0.0 3.12382450899832 2.9159466708345266E-5 80-81 1.749568002500716E-4 5.831893341669053E-5 0.0 3.3798883658976537 2.9159466708345266E-5 82-83 1.749568002500716E-4 5.831893341669053E-5 0.0 3.7205875749179596 4.37392000625179E-5 84-85 1.749568002500716E-4 5.831893341669053E-5 0.0 4.257107182618158 5.831893341669053E-5 86-87 1.749568002500716E-4 8.74784001250358E-5 0.0 4.902493659273965 5.831893341669053E-5 88-89 1.749568002500716E-4 8.74784001250358E-5 0.0 5.63149490671595 5.831893341669053E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3165 0.0 42.324207 2 ACCTGGG 3465 0.0 37.425404 3 TATAACG 550 0.0 36.27438 2 TAACGCA 540 0.0 34.294117 4 GTACCTG 4080 0.0 34.244987 1 ATAACGC 585 0.0 29.231678 3 CCTGGGG 2850 0.0 26.990995 4 GTACACG 400 0.0 26.138008 1 GTATAAC 1070 0.0 25.760485 1 TACGCAG 925 0.0 24.640429 4 TACACGG 455 0.0 24.012083 2 AGTACGC 940 0.0 23.24574 2 GAGTACG 1075 0.0 22.988188 1 ACGCGAT 145 2.3421217E-5 22.923388 8 GTACGCA 960 0.0 22.266315 3 CTGGGGG 2065 0.0 20.695274 5 GTACCCG 325 2.1827873E-11 20.471727 1 ACGTGCG 220 1.3506506E-6 19.425337 9 CCTATAC 1365 0.0 19.139788 3 AGTACTT 11055 0.0 18.877691 12-13 >>END_MODULE