##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003824_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1897512 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.116385034719148 31.0 31.0 34.0 28.0 34.0 2 30.908723106889443 33.0 31.0 34.0 27.0 34.0 3 31.06532448806648 33.0 31.0 34.0 27.0 34.0 4 34.49462717495331 37.0 35.0 37.0 32.0 37.0 5 34.45116763424948 37.0 35.0 37.0 32.0 37.0 6 34.56403859369532 37.0 35.0 37.0 32.0 37.0 7 34.52722934031511 37.0 35.0 37.0 32.0 37.0 8 34.58426718777009 37.0 35.0 37.0 32.0 37.0 9 36.087061900003796 39.0 37.0 39.0 32.0 39.0 10-11 35.99768723465253 39.0 37.0 39.0 32.0 39.0 12-13 35.983656229842026 39.0 37.0 39.0 31.5 39.0 14-15 37.023417243211114 40.0 37.0 41.0 31.0 41.0 16-17 37.00712643714506 40.0 37.0 41.0 31.0 41.0 18-19 36.934737698628524 40.0 37.0 41.0 31.0 41.0 20-21 36.8598425201 40.0 37.0 41.0 30.5 41.0 22-23 36.687616731804596 40.0 36.5 41.0 30.0 41.0 24-25 36.59634537225588 39.5 36.0 41.0 30.0 41.0 26-27 36.45333310145074 39.0 36.0 41.0 30.0 41.0 28-29 36.315276267027556 39.0 36.0 41.0 30.0 41.0 30-31 36.05313774036739 39.0 36.0 40.5 29.0 41.0 32-33 35.79804080290401 39.0 35.0 40.0 27.0 41.0 34-35 35.66340950676465 39.0 35.0 40.0 27.0 41.0 36-37 35.65371655093617 39.0 35.0 40.0 27.0 41.0 38-39 35.60772632794944 39.0 35.0 40.5 27.0 41.0 40-41 35.745355760595984 39.0 35.0 41.0 27.0 41.0 42-43 35.626669291156 39.0 35.0 41.0 27.0 41.0 44-45 35.40061670229227 39.0 35.0 41.0 25.5 41.0 46-47 35.122278278082035 39.0 35.0 41.0 24.0 41.0 48-49 34.83756044757556 39.0 35.0 41.0 23.0 41.0 50-51 34.04485452529417 38.0 33.5 39.5 22.5 40.5 52-53 34.19154714173085 38.0 34.0 40.0 23.0 40.5 54-55 34.53591413387636 39.0 34.0 40.0 23.0 41.0 56-57 34.25483448853024 38.5 34.0 40.0 21.0 41.0 58-59 34.025337916176554 38.0 34.0 40.0 19.5 41.0 60-61 33.56694608518944 38.0 33.0 40.0 16.5 41.0 62-63 33.447499673256345 38.0 33.0 40.0 17.5 41.0 64-65 33.15239376615273 37.0 33.0 40.0 14.0 41.0 66-67 32.74303140111894 36.5 32.0 39.0 12.5 41.0 68-69 32.385186496844284 36.0 32.0 39.0 10.5 41.0 70-71 31.911214527233554 35.5 31.0 39.0 8.0 40.5 72-73 31.50921232645696 35.0 31.0 38.0 7.0 40.0 74-75 31.111478082879053 35.0 31.0 37.0 6.0 40.0 76-77 30.70202059328215 35.0 30.5 37.0 2.0 39.0 78-79 30.123441907086754 34.5 30.0 36.0 2.0 39.0 80-81 29.695921817622235 34.0 29.0 36.0 2.0 38.5 82-83 29.34813824629304 34.0 29.0 35.5 2.0 37.0 84-85 29.092938542681154 34.0 29.0 35.0 2.0 37.0 86-87 28.94581220039715 34.0 29.0 35.0 2.0 36.5 88-89 28.583781815345567 34.0 29.0 35.0 2.0 36.0 90-91 28.289499618447735 34.0 28.0 35.0 2.0 36.0 92-93 28.058291330963915 34.0 27.5 35.0 2.0 35.5 94-95 27.754711169152024 34.0 27.0 35.0 2.0 35.0 96-97 27.518162203980793 34.0 26.0 35.0 2.0 35.0 98-99 27.293745441399054 34.0 25.5 35.0 2.0 35.0 100-101 26.968818906020093 34.0 25.0 35.0 2.0 35.0 102-103 26.652254900100765 33.0 25.0 35.0 2.0 35.0 104-105 26.314593530897298 33.0 24.5 35.0 2.0 35.0 106-107 25.910559985918404 33.0 23.0 35.0 2.0 35.0 108-109 25.69054424952253 32.5 21.5 35.0 2.0 35.0 110-111 25.39755980462838 32.0 19.5 35.0 2.0 35.0 112-113 24.868479883131172 32.0 17.5 35.0 2.0 35.0 114-115 24.388895037290936 32.0 9.0 34.0 2.0 35.0 116-117 23.74935099224669 31.5 2.0 34.0 2.0 35.0 118-119 23.025746082238214 31.0 2.0 34.0 2.0 35.0 120-121 21.461032393998035 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4286168207566625 1101 2 0.5970118175805332 1101 3 0.5510865494330659 1101 4 0.7729014363222007 1101 5 0.7962765513204317 1101 6 0.7493244395496816 1101 7 0.7473523155011108 1101 8 0.7134316747882608 1101 9 0.7894277101237606 1101 10-11 0.6623834796364605 1101 12-13 0.5411682541534972 1101 14-15 0.76471167288517 1101 16-17 0.7531019324484092 1101 18-19 0.8348724731331458 1101 20-21 0.8044136209219204 1101 22-23 0.8942030343112606 1101 24-25 0.8360494399317915 1101 26-27 0.6402319572910358 1101 28-29 0.7548434484981641 1101 30-31 0.6078929094742591 1101 32-33 0.8742169069181358 1101 34-35 0.8420769367312033 1101 36-37 0.6592382948162694 1101 38-39 0.690489598995967 1101 40-41 0.8030429736569289 1101 42-43 0.8598679488337453 1101 44-45 0.8604526707895275 1101 46-47 0.7726132558528462 1101 48-49 1.0171086017289 1101 50-51 0.5367014162125088 1101 52-53 0.766736967540453 1101 54-55 0.8024298050966223 1101 56-57 0.7903715113343495 1101 58-59 0.7104404031775147 1101 60-61 0.8277537887934443 1101 62-63 0.8295057751466643 1101 64-65 0.7889834238182587 1101 66-67 0.6136400828968647 1101 68-69 0.654022010201615 1101 70-71 0.39327794369191693 1101 72-73 0.3125589655992158 1101 74-75 0.49170352674613227 1101 76-77 0.5476277607852076 1101 78-79 0.7718805964361515 1101 80-81 0.7367865953838759 1101 82-83 0.6255448230338558 1101 84-85 0.7632005209294626 1101 86-87 0.8178235874756972 1101 88-89 0.9075695017253729 1101 90-91 0.8783922768063803 1101 92-93 0.869707100255912 1101 94-95 0.9169826375774583 1101 96-97 0.7605731687375226 1101 98-99 0.7871548260450716 1101 100-101 0.8763640907475363 1101 102-103 0.8020361063262875 1101 104-105 0.6718827504610516 1101 106-107 0.532293890918595 1101 108-109 0.7517763071690098 1101 110-111 0.6172917726736209 1101 112-113 0.6153024506897218 1101 114-115 0.6153421785968405 1101 116-117 0.5489883315003645 1101 118-119 0.2794821636900906 1101 120-121 0.4542077285201316 1102 1 0.37155519447092544 1102 2 0.7341655828325422 1102 3 0.7524225390706043 1102 4 0.8249080638322042 1102 5 0.8805595162261781 1102 6 0.8389085243912788 1102 7 0.7523626995304653 1102 8 0.7125146408780907 1102 9 0.7715498834835586 1102 10-11 0.6012904564101476 1102 12-13 0.5525425828707213 1102 14-15 0.6076191334434142 1102 16-17 0.730097744582686 1102 18-19 0.7641851649924121 1102 20-21 0.8579348239215534 1102 22-23 0.7600223873001823 1102 24-25 0.6076205640838523 1102 26-27 0.6864183691193944 1102 28-29 0.7912006607996531 1102 30-31 0.8736180217406044 1102 32-33 0.9438963658237256 1102 34-35 0.8377893750264747 1102 36-37 0.7681843338091383 1102 38-39 0.8022474242321849 1102 40-41 0.7927236462527674 1102 42-43 0.898024234924641 1102 44-45 0.9247609159752628 1102 46-47 1.021953021039721 1102 48-49 0.9629072881523584 1102 50-51 0.9131672009309213 1102 52-53 0.9777338470455419 1102 54-55 0.911625559802637 1102 56-57 0.9541880850994389 1102 58-59 0.8525878483595264 1102 60-61 0.944820451682304 1102 62-63 0.7231632497659533 1102 64-65 0.706574172538275 1102 66-67 0.9426022635472577 1102 68-69 0.9092751322823673 1102 70-71 0.7282749559840234 1102 72-73 0.5874188943255696 1102 74-75 0.8384275568405748 1102 76-77 0.8552778365456994 1102 78-79 1.0435861991602273 1102 80-81 0.9028823272116142 1102 82-83 0.8633822299825091 1102 84-85 0.7595203329639411 1102 86-87 0.8439416097610817 1102 88-89 1.0025682520895884 1102 90-91 0.7816344156246338 1102 92-93 0.8019748318688222 1102 94-95 0.9328123090479998 1102 96-97 0.9019161740967867 1102 98-99 1.1074108935448557 1102 100-101 1.075108090487042 1102 102-103 0.8598574519662456 1102 104-105 0.8152561496691462 1102 106-107 1.0208880570013221 1102 108-109 0.7977641728037703 1102 110-111 0.6486365922249462 1102 112-113 0.8826048316793589 1102 114-115 0.5440980319035518 1102 116-117 0.26480984122109064 1102 118-119 0.23140575702519328 1102 120-121 0.15292381761260998 1103 1 0.5868889569551001 1103 2 0.7993424722705811 1103 3 0.7733076795911984 1103 4 0.9702154572143371 1103 5 1.130978051033054 1103 6 1.03952406649109 1103 7 0.9748184870354137 1103 8 0.9400810061245437 1103 9 0.9685934776180929 1103 10-11 0.9446533680821076 1103 12-13 0.8371397128211271 1103 14-15 0.9381730198702556 1103 16-17 0.9473239044455113 1103 18-19 1.0598682532550612 1103 20-21 1.1145509432329987 1103 22-23 1.2103060890756723 1103 24-25 1.1358610944872325 1103 26-27 0.892148209270097 1103 28-29 0.9563593996373143 1103 30-31 0.9300970532877031 1103 32-33 0.9465690135453713 1103 34-35 1.0329315528504992 1103 36-37 0.9880524770252634 1103 38-39 0.8249150438993169 1103 40-41 1.045908174726101 1103 42-43 0.9614222527888856 1103 44-45 1.0154882139145442 1103 46-47 0.8644822451973795 1103 48-49 0.8798936571642102 1103 50-51 0.7072558547764274 1103 52-53 0.8100903924789762 1103 54-55 0.9441031843963614 1103 56-57 0.9748161860618652 1103 58-59 0.8134154706421484 1103 60-61 0.9142861814322671 1103 62-63 0.8694597945004219 1103 64-65 0.9185940412451785 1103 66-67 0.8731729477347017 1103 68-69 0.709524731221741 1103 70-71 0.39257103398876225 1103 72-73 0.42329721991577784 1103 74-75 0.6358340502460429 1103 76-77 0.6925333530317452 1103 78-79 0.9065210948731952 1103 80-81 0.7183557704030505 1103 82-83 0.737446374880669 1103 84-85 0.8797950045339 1103 86-87 0.8962013595082716 1103 88-89 0.9442486820220708 1103 90-91 0.8953118512717388 1103 92-93 0.8202564322331831 1103 94-95 0.7115304482619749 1103 96-97 0.6256059769542084 1103 98-99 0.7743445581736204 1103 100-101 0.6403646847354416 1103 102-103 0.705096639676146 1103 104-105 0.7414316828853131 1103 106-107 0.8675719648594082 1103 108-109 0.9605192535626692 1103 110-111 0.9420133141363252 1103 112-113 1.0121863645144735 1103 114-115 0.7454055515507321 1103 116-117 0.84823270624263 1103 118-119 0.791547489539461 1103 120-121 0.6087506676491898 1104 1 0.6405108746091628 1104 2 0.8017042561042977 1104 3 0.8486920497118433 1104 4 1.0093815885683384 1104 5 1.1567644917587074 1104 6 1.0164503715208397 1104 7 1.091365978255503 1104 8 0.9742103532931665 1104 9 0.9366756359987463 1104 10-11 1.000646798975204 1104 12-13 0.9725664703268322 1104 14-15 1.1253196549026825 1104 16-17 1.091684483239625 1104 18-19 1.0078099565339897 1104 20-21 0.9829007188705674 1104 22-23 1.1703164749227852 1104 24-25 1.0660121698795777 1104 26-27 1.075591105866451 1104 28-29 0.9185808221467084 1104 30-31 1.0925680409044034 1104 32-33 1.2630166318338425 1104 34-35 1.087044864083623 1104 36-37 0.9573927764565084 1104 38-39 1.0147872018579633 1104 40-41 1.2445152286621664 1104 42-43 1.198431152264213 1104 44-45 1.1482376266335805 1104 46-47 1.0759134758851019 1104 48-49 1.1023295485887346 1104 50-51 1.0123896985714111 1104 52-53 1.0660028231423055 1104 54-55 1.0341315989487398 1104 56-57 1.2270155005490722 1104 58-59 1.0666582931543473 1104 60-61 1.1922415762116785 1104 62-63 1.2730112618822957 1104 64-65 1.3427416046738685 1104 66-67 1.2343550822021143 1104 68-69 1.1226693922398852 1104 70-71 0.8382549291268724 1104 72-73 1.0363131714516136 1104 74-75 1.0557742905975225 1104 76-77 1.071467666651504 1104 78-79 1.0598667208607857 1104 80-81 0.8961013382486556 1104 82-83 0.8300574190451364 1104 84-85 0.9176738066213872 1104 86-87 0.8257735938955051 1104 88-89 0.8766631921992634 1104 90-91 0.9967842736826604 1104 92-93 1.004093428355489 1104 94-95 0.9067155648507281 1104 96-97 0.9684245036174737 1104 98-99 1.0018520486434923 1104 100-101 0.8938305802392819 1104 102-103 0.8811305355175882 1104 104-105 0.9646881265649512 1104 106-107 0.7569910607514885 1104 108-109 0.7707554085016923 1104 110-111 0.7771207595930463 1104 112-113 0.812912436038495 1104 114-115 0.800145530263265 1104 116-117 0.8506609548090083 1104 118-119 0.8811479274799225 1104 120-121 0.9585149347084752 1105 1 0.6911290988004417 1105 2 0.948232735380163 1105 3 0.810606337471441 1105 4 1.0546209242507558 1105 5 1.161553790455045 1105 6 1.147056943110762 1105 7 1.0370362215681794 1105 8 0.9721522573202748 1105 9 1.0728171932317068 1105 10-11 1.1882357842841742 1105 12-13 0.9536414327442131 1105 14-15 1.2404969693161192 1105 16-17 1.2566095566152242 1105 18-19 1.2017497090056963 1105 20-21 1.1670560643523018 1105 22-23 1.2540312685564032 1105 24-25 1.2958592344580566 1105 26-27 1.148073368459606 1105 28-29 1.1574149058068173 1105 30-31 1.2304137413728924 1105 32-33 1.3431645562390813 1105 34-35 1.287964743501071 1105 36-37 1.0595086383115344 1105 38-39 1.1429817247376306 1105 40-41 1.1680674028864573 1105 42-43 1.192465656690608 1105 44-45 1.1869616469003503 1105 46-47 1.2365950934093348 1105 48-49 1.2742844613731847 1105 50-51 1.1326491180889136 1105 52-53 1.1883282926268137 1105 54-55 1.2100182704408198 1105 56-57 1.4558543963756065 1105 58-59 1.2640406336966592 1105 60-61 1.3136861408498106 1105 62-63 1.0822511185513193 1105 64-65 1.2083479376439712 1105 66-67 1.2809103067831416 1105 68-69 1.1135153392821877 1105 70-71 1.053018238147498 1105 72-73 1.0802137505023843 1105 74-75 1.2097790272010052 1105 76-77 1.170666898295675 1105 78-79 1.2473070659050514 1105 80-81 1.3370762394202806 1105 82-83 1.170204508280996 1105 84-85 1.2614419516704203 1105 86-87 1.2926964123137736 1105 88-89 1.2505138400520828 1105 90-91 1.2739799737534518 1105 92-93 1.2528695075136405 1105 94-95 1.1822359549145993 1105 96-97 1.1104147321357196 1105 98-99 1.1297786213469756 1105 100-101 1.265413842649501 1105 102-103 1.0290106966655088 1105 104-105 1.0982341960987654 1105 106-107 1.2359159591392128 1105 108-109 1.3650152098109167 1105 110-111 1.2306092819795609 1105 112-113 1.246902239018059 1105 114-115 1.1833654860390581 1105 116-117 1.1300494716277285 1105 118-119 1.0228592690801044 1105 120-121 1.0944372599791095 1106 1 0.6277174131278649 1106 2 0.830017912335208 1106 3 0.8176511177950161 1106 4 0.9743034424649082 1106 5 1.1966533395443975 1106 6 1.1329852668722111 1106 7 1.1026395856254396 1106 8 1.0770538148287088 1106 9 1.0812415880121478 1106 10-11 1.108962304850131 1106 12-13 1.0005291823714302 1106 14-15 1.122607838118327 1106 16-17 1.22570976966324 1106 18-19 1.2094077289333924 1106 20-21 1.2604220385942853 1106 22-23 1.267887773004034 1106 24-25 1.0246216389720786 1106 26-27 1.0958664044944584 1106 28-29 1.0265380611977335 1106 30-31 1.1543773398947437 1106 32-33 1.2082243181081793 1106 34-35 1.2523216760567308 1106 36-37 1.1738083128152965 1106 38-39 1.2418973148001626 1106 40-41 1.2171251739973314 1106 42-43 1.312826888210978 1106 44-45 1.0907804152458382 1106 46-47 1.079862004794336 1106 48-49 1.146744735630591 1106 50-51 1.135179482461389 1106 52-53 1.1533477866321746 1106 54-55 1.1095431040599308 1106 56-57 1.193509823064069 1106 58-59 1.2526622376151266 1106 60-61 1.3951842730468869 1106 62-63 1.3069523650945953 1106 64-65 1.3877542647006251 1106 66-67 1.3344236709074622 1106 68-69 1.1798441396631318 1106 70-71 0.8843737142416295 1106 72-73 1.0701636909249537 1106 74-75 1.173761644673629 1106 76-77 1.219626864047033 1106 78-79 1.2551637704344998 1106 80-81 1.480377438569878 1106 82-83 1.292434520777725 1106 84-85 1.3418576579816062 1106 86-87 1.249999482619618 1106 88-89 1.4899201223977094 1106 90-91 1.156996939350556 1106 92-93 1.0182738516364864 1106 94-95 1.1408299162440372 1106 96-97 1.1118660239489095 1106 98-99 1.163260609511969 1106 100-101 1.1047262039056136 1106 102-103 1.0307948061449146 1106 104-105 0.8785900455299291 1106 106-107 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114-115 1.3315538448671589 2204 116-117 1.365943001872438 2204 118-119 1.1313782455664132 2204 120-121 1.0749210457033662 2205 1 0.7896928157852088 2205 2 1.176399305651998 2205 3 1.0326518358327306 2205 4 1.084792507053919 2205 5 1.23264497996729 2205 6 1.1561662548516551 2205 7 1.1446369541689165 2205 8 1.0671734641835897 2205 9 1.215168263214359 2205 10-11 1.1928627362795439 2205 12-13 1.060940931491082 2205 14-15 1.1904801002202916 2205 16-17 1.308915803000417 2205 18-19 1.3610662696380444 2205 20-21 1.3536282640824027 2205 22-23 1.3901407730869622 2205 24-25 1.3827832753294658 2205 26-27 1.254854744977827 2205 28-29 1.2880058061345565 2205 30-31 1.1702031186622648 2205 32-33 1.3292475521904947 2205 34-35 1.3307520001041127 2205 36-37 1.233067535554646 2205 38-39 1.1752860432261656 2205 40-41 1.237471682817059 2205 42-43 1.2399039639973282 2205 44-45 1.173888097642589 2205 46-47 1.2047366010245355 2205 48-49 1.3065887798617197 2205 50-51 1.253540288322192 2205 52-53 1.2944228559582172 2205 54-55 1.163483664695697 2205 56-57 1.2853680844419344 2205 58-59 1.376593650854936 2205 60-61 1.4390958188910687 2205 62-63 1.142329198366241 2205 64-65 1.2755953831808924 2205 66-67 1.2525099790407097 2205 68-69 1.1818351773388684 2205 70-71 1.1151085601062398 2205 72-73 1.1492204017458754 2205 74-75 1.2509637197014847 2205 76-77 1.3273085023057014 2205 78-79 1.4974998555715224 2205 80-81 1.3123389153408525 2205 82-83 1.2122206062181462 2205 84-85 1.2211971087940015 2205 86-87 1.2023865029249166 2205 88-89 1.3622715998361592 2205 90-91 1.2835110128897576 2205 92-93 0.3112968258093787 2205 94-95 0.9635883744249121 2205 96-97 1.0973411828779582 2205 98-99 1.1385746498798461 2205 100-101 1.2509064202473468 2205 102-103 1.2402479243501041 2205 104-105 1.0212870204673763 2205 106-107 1.3126944438124326 2205 108-109 1.3695337176189 2205 110-111 1.4507027849678025 2205 112-113 1.3903738989370886 2205 114-115 1.3330064152852472 2205 116-117 1.3956654346121127 2205 118-119 1.2526284034545014 2205 120-121 1.1522862092754238 2206 1 0.6863590927706369 2206 2 0.9802337690609306 2206 3 0.7994081291847692 2206 4 0.9776009397559662 2206 5 1.1625120986641946 2206 6 0.986362654196995 2206 7 1.021897105365234 2206 8 0.9257873680102549 2206 9 0.7813945900789818 2206 10-11 1.0128007769622727 2206 12-13 0.916466611484843 2206 14-15 1.0860747537936746 2206 16-17 0.9915350454600826 2206 18-19 1.0699500277239267 2206 20-21 1.1339405935968543 2206 22-23 1.259490395096158 2206 24-25 1.2439544622985252 2206 26-27 1.144482404552292 2206 28-29 0.9471954639944258 2206 30-31 1.058564314204311 2206 32-33 1.1285519711757672 2206 34-35 1.2853590065728255 2206 36-37 1.05198753399592 2206 38-39 1.1404728953811016 2206 40-41 1.1936617693262903 2206 42-43 1.145303971790959 2206 44-45 1.2645523093174447 2206 46-47 1.362391888998971 2206 48-49 1.4533427351263057 2206 50-51 1.2172171256692437 2206 52-53 1.3403224351024718 2206 54-55 1.261809983766689 2206 56-57 1.3751896104336723 2206 58-59 1.4007879848896962 2206 60-61 1.402173521330468 2206 62-63 1.3135210384410598 2206 64-65 1.3652951337848904 2206 66-67 1.4106426265350507 2206 68-69 1.32586136391825 2206 70-71 1.3570226743607812 2206 72-73 1.0950352252202826 2206 74-75 1.1285112608128607 2206 76-77 1.2716230530032284 2206 78-79 1.231021210103517 2206 80-81 1.2266285264389722 2206 82-83 1.184358397504873 2206 84-85 1.1104545711897629 2206 86-87 1.0861056206865847 2206 88-89 1.1729985501907692 2206 90-91 1.0551626768286546 2206 92-93 1.0571808177040083 2206 94-95 1.2801556723751908 2206 96-97 1.110570861001257 2206 98-99 1.0779061517113462 2206 100-101 1.3005859074268322 2206 102-103 1.1962139441459136 2206 104-105 1.1628338601525172 2206 106-107 1.3444632069641784 2206 108-109 1.2932390016965236 2206 110-111 1.3055026836506798 2206 112-113 1.3784058138307067 2206 114-115 1.168823966528329 2206 116-117 0.5048395052223533 2206 118-119 0.6006868166724892 2206 120-121 0.6155684943662223 2207 1 0.5869774253065501 2207 2 1.038768192030993 2207 3 0.9331134915597623 2207 4 1.0612433589259993 2207 5 1.1356698896265698 2207 6 1.0694090777406444 2207 7 1.1232263583059208 2207 8 1.0276684942868712 2207 9 1.2017745381525629 2207 10-11 1.171908858649175 2207 12-13 0.9874555850486999 2207 14-15 1.2263548545028016 2207 16-17 1.2478310894830997 2207 18-19 1.304552210122715 2207 20-21 1.277346482819567 2207 22-23 1.3478009870656251 2207 24-25 1.236265636130696 2207 26-27 1.2049997453018477 2207 28-29 1.2192097292824542 2207 30-31 1.3168063925296494 2207 32-33 1.3952348504861334 2207 34-35 1.4156886690235169 2207 36-37 1.355286916145964 2207 38-39 1.4352970060067136 2207 40-41 1.547896199328349 2207 42-43 1.5036774518125213 2207 44-45 1.4729546411804861 2207 46-47 1.5448454882058726 2207 48-49 1.6720246988991505 2207 50-51 1.6246487326720072 2207 52-53 1.4712000067770674 2207 54-55 1.3792973020552424 2207 56-57 1.5535126049032115 2207 58-59 1.378148768722177 2207 60-61 1.5149573345166942 2207 62-63 1.5448057761271556 2207 64-65 1.6458370658172612 2207 66-67 1.7583674675224614 2207 68-69 1.4170030212893607 2207 70-71 1.3518969051506922 2207 72-73 1.589200914072645 2207 74-75 1.6882376750356762 2207 76-77 1.7528915895357962 2207 78-79 1.7957193102252695 2207 80-81 1.7651279824938584 2207 82-83 1.6245592504439017 2207 84-85 1.5595186615742733 2207 86-87 1.47752066390575 2207 88-89 1.7078246532135601 2207 90-91 1.6751563480376177 2207 92-93 1.594134442419108 2207 94-95 1.4175709265540526 2207 96-97 1.4440593692128267 2207 98-99 1.6611638796615757 2207 100-101 1.741022366502719 2207 102-103 1.558879868087356 2207 104-105 1.672060934141463 2207 106-107 1.621718717769312 2207 108-109 1.7896406220876422 2207 110-111 1.803401704273714 2207 112-113 1.6168538743113352 2207 114-115 1.7775745542510286 2207 116-117 1.7820970842598527 2207 118-119 1.5720331807889352 2207 120-121 1.3846670690783611 2208 1 0.1673642329097511 2208 2 0.7398350886190315 2208 3 0.7480335894957335 2208 4 1.0925463417608725 2208 5 1.047734838025896 2208 6 0.9789426971593471 2208 7 1.0815374234912625 2208 8 0.9408184390004379 2208 9 1.0233928511264452 2208 10-11 0.9675729869663598 2208 12-13 0.740036770547853 2208 14-15 1.0751155168869602 2208 16-17 1.03261300423668 2208 18-19 0.9922118167992124 2208 20-21 1.0214367576721415 2208 22-23 1.1261347920887914 2208 24-25 0.9636998847617022 2208 26-27 0.9892898411316722 2208 28-29 0.9687335419404235 2208 30-31 0.9902845346421216 2208 32-33 1.098087696421267 2208 34-35 1.0117501686022905 2208 36-37 0.9723067468641702 2208 38-39 1.0589949775210314 2208 40-41 0.9944235200423321 2208 42-43 0.9202854314881748 2208 44-45 0.8395932258477217 2208 46-47 0.9153171012534642 2208 48-49 1.002497233387352 2208 50-51 0.9783435449613904 2208 52-53 1.0721829864527237 2208 54-55 1.0376909678092474 2208 56-57 1.024897760495044 2208 58-59 1.0354056071278208 2208 60-61 1.0978104766291636 2208 62-63 0.9678786489156934 2208 64-65 1.0185777263585436 2208 66-67 1.1051077068337491 2208 68-69 1.135080369646559 2208 70-71 1.143247868714301 2208 72-73 1.0952733722129118 2208 74-75 1.1324526734560685 2208 76-77 1.2045714086701658 2208 78-79 1.1557766699030232 2208 80-81 1.3056846971631941 2208 82-83 1.211343488544152 2208 84-85 1.222089250506457 2208 86-87 1.037967003153856 2208 88-89 1.2143849812464147 2208 90-91 1.2142902596846277 2208 92-93 1.2413413255804393 2208 94-95 1.174642489580588 2208 96-97 1.134140061083091 2208 98-99 1.285808795942117 2208 100-101 1.2016676381960636 2208 102-103 1.1502017075460742 2208 104-105 1.2469745490845945 2208 106-107 1.2013899280157325 2208 108-109 1.1622341812178014 2208 110-111 1.1231565681637719 2208 112-113 1.3027987787447834 2208 114-115 1.3666745700471523 2208 116-117 1.1231637175791462 2208 118-119 1.2636653482805116 2208 120-121 1.1602646891289083 2209 1 0.6315110630217085 2209 2 1.022632075839141 2209 3 1.0084314427910464 2209 4 1.0890178444541547 2209 5 1.0625561440039277 2209 6 1.0610848470326033 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2209 72-73 1.6712558757775504 2209 74-75 1.7709149608979793 2209 76-77 1.7314813784367509 2209 78-79 1.381909515408939 2209 80-81 1.5678357421304554 2209 82-83 1.4848613579463503 2209 84-85 1.6182433188154945 2209 86-87 1.682785487126182 2209 88-89 1.671095248726349 2209 90-91 1.7346521035270435 2209 92-93 1.7465347237373905 2209 94-95 1.9068756532635227 2209 96-97 1.7287676883538943 2209 98-99 1.6891310062822065 2209 100-101 1.6379774449134246 2209 102-103 1.632680270204503 2209 104-105 1.576203349385608 2209 106-107 1.6678578165613587 2209 108-109 1.9306716977207579 2209 110-111 1.8726820256257497 2209 112-113 2.0177523559258113 2209 114-115 2.013011243579051 2209 116-117 1.882609020177373 2209 118-119 1.6846217111607373 2209 120-121 1.4948350841436913 2210 1 0.380615238920349 2210 2 0.6221617746418495 2210 3 0.5978470318347711 2210 4 0.7672989777633177 2210 5 0.8978551772679495 2210 6 0.8902424883166447 2210 7 0.7455811403820505 2210 8 0.6915420662416736 2210 9 0.725859487230764 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1.0330311618792152 2210 78-79 0.8304743891878665 2210 80-81 0.9133849760446147 2210 82-83 0.8468897268285858 2210 84-85 1.0290668572199806 2210 86-87 0.9013216346228852 2210 88-89 1.000762133345031 2210 90-91 0.9522190876186123 2210 92-93 1.0129831285042386 2210 94-95 1.1247403845989261 2210 96-97 1.131923654196374 2210 98-99 1.0233797897277732 2210 100-101 1.115809090926298 2210 102-103 1.2696236016475417 2210 104-105 1.1839710662504466 2210 106-107 1.2674050754790578 2210 108-109 1.4114741020103843 2210 110-111 1.2693510784021598 2210 112-113 1.483099276656354 2210 114-115 1.6142964374065656 2210 116-117 1.3221226876280454 2210 118-119 1.123187907113028 2210 120-121 1.1451796239292307 2211 1 0.39178908474750784 2211 2 0.6930548988626164 2211 3 0.6030829995653306 2211 4 0.8748061218282928 2211 5 0.901436515385619 2211 6 0.7772473268896647 2211 7 0.7879479892263603 2211 8 0.6415988394462815 2211 9 0.7181521214613724 2211 10-11 0.6897707325894586 2211 12-13 0.5570496871692114 2211 14-15 0.7278514210844378 2211 16-17 0.6336296656927516 2211 18-19 0.8122889902985548 2211 20-21 0.7664491951449577 2211 22-23 0.4231432570463838 2211 24-25 0.6038673148943658 2211 26-27 0.5917382562034419 2211 28-29 0.6808092745959158 2211 30-31 0.7954043727510793 2211 32-33 0.7599615750097826 2211 34-35 0.8197427740829326 2211 36-37 0.7201491341793016 2211 38-39 0.8569167131941526 2211 40-41 0.8297806895997439 2211 42-43 0.8063236886108385 2211 44-45 0.7639516425506301 2211 46-47 0.8532637118722164 2211 48-49 0.9831189880904887 2211 50-51 0.8193900087771766 2211 52-53 0.9313060086906475 2211 54-55 1.0711535466478708 2211 56-57 0.945245686903263 2211 58-59 0.561987901739613 2211 60-61 0.8124983334419085 2211 62-63 1.0003112282980382 2211 64-65 0.9226551615205736 2211 66-67 0.9565155030223025 2211 68-69 1.0775465234090689 2211 70-71 0.9988579845255181 2211 72-73 0.8910282350608902 2211 74-75 0.9795932440846009 2211 76-77 0.8848528319792166 2211 78-79 0.6173446240406939 2211 80-81 0.697439859054235 2211 82-83 0.6625653173284078 2211 84-85 0.5207499283827062 2211 86-87 0.6764355798448065 2211 88-89 0.7575737657722961 2211 90-91 0.6214052757242072 2211 92-93 0.8631557523692628 2211 94-95 0.9843280016238687 2211 96-97 0.9404767156067955 2211 98-99 1.0304775038042315 2211 100-101 1.1385212973957124 2211 102-103 0.9159243213183679 2211 104-105 0.765106498437575 2211 106-107 0.8620619423426135 2211 108-109 1.0499116530805672 2211 110-111 1.2456429922883956 2211 112-113 1.2497379200834082 2211 114-115 1.3606478328788185 2211 116-117 1.3378593053346926 2211 118-119 1.156186468196779 2211 120-121 1.075593364639996 2212 1 -0.019712399005587855 2212 2 0.15027161942372658 2212 3 0.20940788224510598 2212 4 0.3241516042294421 2212 5 0.45836913706193627 2212 6 0.3189265875616982 2212 7 0.3599777393965411 2212 8 0.324890565467328 2212 9 0.3644801834818523 2212 10-11 0.41918869447893314 2212 12-13 0.25660919170250907 2212 14-15 0.24524400075117114 2212 16-17 0.37614116983810675 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60-61 -0.5403010236750561 2313 62-63 -0.40898046411837896 2313 64-65 -0.2544692542728839 2313 66-67 -0.35585841928172357 2313 68-69 -0.2757354429548471 2313 70-71 -0.3441308664128009 2313 72-73 -0.35979471780196093 2313 74-75 -0.3086335133340903 2313 76-77 -0.26081729383794183 2313 78-79 -0.7797722120939987 2313 80-81 -0.541418783603433 2313 82-83 -0.4554128239896471 2313 84-85 -0.19272861979506217 2313 86-87 -0.10623393738832831 2313 88-89 0.09404015803116295 2313 90-91 0.16649436494422432 2313 92-93 -0.03520036502935042 2313 94-95 0.05376433331369057 2313 96-97 -0.0646781345632057 2313 98-99 0.08834838659922539 2313 100-101 0.16906471093808406 2313 102-103 0.07722296481351876 2313 104-105 0.4359237065683672 2313 106-107 0.3815042845936105 2313 108-109 0.5489925430011979 2313 110-111 0.4594260677243476 2313 112-113 0.3778073348899973 2313 114-115 0.4238148316750596 2313 116-117 0.30961543134334235 2313 118-119 0.15195684452848113 2313 120-121 0.677840067711923 2314 1 -0.6299863197538507 2314 2 -5.284954916113257 2314 3 -2.1075467072863105 2314 4 -0.7501868501671964 2314 5 -3.115556552590654 2314 6 -0.8433865179569295 2314 7 -0.17673837592734287 2314 8 -0.06536501320345423 2314 9 -0.05616789116668741 2314 10-11 0.15863202648096575 2314 12-13 0.06694605850756119 2314 14-15 0.1850267019870202 2314 16-17 0.10189084445436691 2314 18-19 0.28535697127460935 2314 20-21 0.1816893435293565 2314 22-23 0.20176699615180382 2314 24-25 0.1553104259326048 2314 26-27 0.07021491887640252 2314 28-29 0.10131977943365911 2314 30-31 -0.08607230097848628 2314 32-33 0.07941396230823727 2314 34-35 0.07628505232321459 2314 36-37 0.024149909553450755 2314 38-39 -0.040297397580367544 2314 40-41 0.019971430230270926 2314 42-43 0.07316684542814045 2314 44-45 0.23824114364112603 2314 46-47 0.2024303846161004 2314 48-49 0.22742783354284057 2314 50-51 0.0973211806766372 2314 52-53 0.12235516567121607 2314 54-55 0.1097151193776611 2314 56-57 0.13986224721083573 2314 58-59 0.23565183397341372 2314 60-61 0.13141341656291416 2314 62-63 -0.03959160527575989 2314 64-65 0.21713701938855934 2314 66-67 0.18470404691622377 2314 68-69 0.19884541156110913 2314 70-71 0.2578161319647059 2314 72-73 0.05421285385839525 2314 74-75 0.23544436660101198 2314 76-77 0.17996150551305945 2314 78-79 0.07203957806284222 2314 80-81 0.1822156133679016 2314 82-83 0.11002795480970207 2314 84-85 0.05226867604052643 2314 86-87 -0.019700676166049647 2314 88-89 0.19517590708470678 2314 90-91 0.18758684736715736 2314 92-93 0.0050916847272795 2314 94-95 0.15246633439535628 2314 96-97 0.16382375835188512 2314 98-99 0.1497329187787848 2314 100-101 0.22747466226312696 2314 102-103 0.42497850835056283 2314 104-105 0.2725921760113117 2314 106-107 0.38258595035889087 2314 108-109 0.43298388967507506 2314 110-111 0.4313027578271047 2314 112-113 0.5752113370533287 2314 114-115 0.5787634525512075 2314 116-117 0.4258945011107862 2314 118-119 0.33205419444735895 2314 120-121 0.3814636480256084 2315 1 -0.07580423165133965 2315 2 0.14293691987644408 2315 3 -0.06748112728955746 2315 4 0.24186008360548072 2315 5 0.4108400055822443 2315 6 0.1767657621059655 2315 7 0.21241718698473733 2315 8 0.16891795338359117 2315 9 0.09222625859975864 2315 10-11 0.10050387924098203 2315 12-13 0.028185727027683072 2315 14-15 -0.7730430314522962 2315 16-17 -0.21419550147209776 2315 18-19 0.1560773824872328 2315 20-21 0.06585679770772401 2315 22-23 0.18902101502770563 2315 24-25 -0.013789828316596697 2315 26-27 0.160246432175569 2315 28-29 0.2378537784469401 2315 30-31 0.17455782386216612 2315 32-33 0.1571971826312364 2315 34-35 0.06960900608746101 2315 36-37 -0.016773593067625825 2315 38-39 0.244960511699567 2315 40-41 0.19206053998598804 2315 42-43 0.043323038991140095 2315 44-45 0.0014556220517576435 2315 46-47 -0.0018108550780908672 2315 48-49 -0.1818339776891591 2315 50-51 -0.35206844161927364 2315 52-53 -0.2871971106706468 2315 54-55 -0.21000420567830247 2315 56-57 -0.516559229051218 2315 58-59 -0.6528985799273315 2315 60-61 -0.35297332193252373 2315 62-63 -0.33246239497517394 2315 64-65 -0.47271569186506923 2315 66-67 -0.3971435998833357 2315 68-69 -0.33596982311737733 2315 70-71 -0.5006606054849918 2315 72-73 -0.46357175864414657 2315 74-75 -0.1882977157788055 2315 76-77 -0.3759800537408644 2315 78-79 -0.46886259277490794 2315 80-81 -0.6802307925313471 2315 82-83 -0.4806313223989065 2315 84-85 -0.41719126736127876 2315 86-87 -0.3334907277921708 2315 88-89 -0.24482644743092763 2315 90-91 -0.5080244830496561 2315 92-93 -0.44041491144506395 2315 94-95 -0.4029433737794328 2315 96-97 -0.5281606877306722 2315 98-99 -2.3263506235418703 2315 100-101 -11.748449132266572 2315 102-103 -4.686742937694138 2315 104-105 -1.8967149804972578 2315 106-107 -0.8020791278116697 2315 108-109 -0.5620334548290558 2315 110-111 -0.4790312561986241 2315 112-113 -0.39856099475759166 2315 114-115 -0.347937492526988 2315 116-117 -0.34205258580163544 2315 118-119 0.022928906628671086 2315 120-121 -0.02414738085923318 2316 1 -1.4361099505381851 2316 2 -3.6151261302742235 2316 3 -1.5144382711787898 2316 4 -3.7751632706327065 2316 5 -4.115337669341493 2316 6 -1.8317814555911411 2316 7 -7.883748625542793 2316 8 -3.4246108647814886 2316 9 -2.248818797531051 2316 10-11 -1.1878764492238787 2316 12-13 -0.9973091026921637 2316 14-15 -4.8489763503363505 2316 16-17 -3.473557821933916 2316 18-19 -5.161066926242697 2316 20-21 -1.6923612326684037 2316 22-23 -3.6468904667930033 2316 24-25 -1.3699734227857832 2316 26-27 -3.7906498895493996 2316 28-29 -2.1889011686849926 2316 30-31 -1.008774460732802 2316 32-33 -0.7353461710476239 2316 34-35 -0.7672445923669358 2316 36-37 -0.8424598758028239 2316 38-39 -1.045543511959977 2316 40-41 -0.781761785090282 2316 42-43 -0.9368831895825522 2316 44-45 -1.0027119476951754 2316 46-47 -0.6325017173041658 2316 48-49 -0.7775820427668734 2316 50-51 -0.6765428489073102 2316 52-53 -0.7178871572113295 2316 54-55 -0.8124630310447145 2316 56-57 -0.7883741523651366 2316 58-59 -1.0481092248548194 2316 60-61 -0.8726732895381559 2316 62-63 -0.962172104194984 2316 64-65 -1.153902141233143 2316 66-67 -1.0324261882628463 2316 68-69 -0.9273469797313041 2316 70-71 -0.807291894710719 2316 72-73 -0.9424851258633815 2316 74-75 -0.9317011514456972 2316 76-77 -0.8460175985744307 2316 78-79 -0.9999728338215981 2316 80-81 -1.0687807835588075 2316 82-83 -1.1111672043995284 2316 84-85 -1.3290517991415491 2316 86-87 -1.3264857381280706 2316 88-89 -1.1265293421818754 2316 90-91 -1.04062568047582 2316 92-93 -0.7378843057636963 2316 94-95 -0.8726721234269412 2316 96-97 -0.7858322088907137 2316 98-99 -0.5893398308588012 2316 100-101 -0.7378819279467024 2316 102-103 -0.6837843302322462 2316 104-105 -0.6067755276590177 2316 106-107 -0.6372460053912761 2316 108-109 -0.956078252271066 2316 110-111 -0.922289849444283 2316 112-113 -1.075594440189402 2316 114-115 -0.8391799445239023 2316 116-117 -0.38304893386158767 2316 118-119 -0.5513176913805893 2316 120-121 -1.1317764863786266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28000.0 3 5023.0 4 4500.0 5 4880.0 6 5627.0 7 6034.0 8 6928.0 9 7719.0 10 9090.0 11 10992.0 12 11335.0 13 12971.0 14 16684.0 15 17082.0 16 12748.0 17 11936.0 18 12396.0 19 13133.0 20 14124.0 21 15470.0 22 17409.0 23 19449.0 24 22254.0 25 25374.0 26 29941.0 27 34908.0 28 40777.0 29 47913.0 30 57336.0 31 69809.0 32 87371.0 33 111018.0 34 147833.0 35 199403.0 36 282810.0 37 341428.0 38 135051.0 39 756.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.581083914071364 18.998147024545982 13.977159133574235 21.443609927808414 2 13.668253263910394 22.093233604363725 39.325052381119605 24.91346075060628 3 21.942895205727613 25.815640267567996 30.244408774859714 21.997055751844677 4 11.04473991741485 16.043625555119974 42.436033210655964 30.475601316809207 5 10.838193873724357 38.98156053267799 35.829323886198196 14.350921707399458 6 26.50798413387469 37.98427219257255 18.96577589949184 16.54196777406093 7 25.090353227265695 33.04941543655967 23.986328539492938 17.873902796681705 8 23.14048575782316 31.49118024843696 21.128417603867767 24.239916389872114 9 22.502669860571583 20.014966110853464 25.17253043215576 32.309833596419196 10-11 23.944636052561396 24.990062110810324 28.976013192730704 22.08928864389758 12-13 27.240349313582428 23.4076739654813 27.97478633205849 21.377190388877782 14-15 22.162243079317733 25.688226098974802 29.818180947992484 22.33134987371498 16-17 19.514835794197484 29.13405364549993 29.85642247912239 21.494688081180193 18-19 18.813787335335068 31.22285733743399 29.82158101212719 20.14177431510375 20-21 20.886230832414764 27.500244715215327 28.238832474347465 23.374691978022447 22-23 19.385258544576466 27.632862446413565 29.46269958620103 23.51917942280894 24-25 22.1457404826992 27.240381649346368 28.902279471987917 21.711598395966515 26-27 21.216234292399054 25.36663368224487 32.41936080073163 20.997771224624444 28-29 19.232516643595634 29.886412846291595 30.304723833884307 20.576346676228464 30-31 20.17912312240736 27.417095436744287 29.348382981281667 23.055398459566685 32-33 20.752678876607426 27.306493807030446 30.314708984294477 21.626118332067655 34-35 22.634737547750028 26.09684078357931 29.935730322731676 21.332691345938986 36-37 21.680606853735384 25.705059358087173 31.92848669401407 20.685847094163375 38-39 20.77512830532691 27.933971004977927 31.089591739938076 20.201308949757088 40-41 20.136998364139078 28.898577952936478 30.057727347822233 20.90669633510221 42-43 19.29860450862519 29.09635961316334 28.069971956098072 23.535063922113398 44-45 20.563354957934827 26.93056819303142 28.37951667672493 24.126560172308825 46-47 22.32706008393058 28.470966605971533 28.740903043640387 20.461070266457504 48-49 21.742970834345137 27.645999161125367 29.240738667146832 21.370291337382664 50-51 18.777148550698616 28.409642521529328 31.652959233585992 21.160249694186064 52-53 20.1912882968905 29.80472209736234 28.163025929777618 21.84096367596954 54-55 19.244941838087215 27.878749349301046 29.239544727523054 23.63676408508868 56-57 21.733474428459633 26.40406192185079 28.79388944928997 23.068574200399606 58-59 23.703052019449196 26.39177572500194 28.150152663490402 21.755019592058467 60-61 21.554591394713707 27.299289456150845 31.08357936996175 20.0625397791737 62-63 20.02456816188599 28.060346811153135 30.298800027770856 21.616284999190015 64-65 19.276185804919518 30.92383781489091 28.666204200708805 21.13377217948077 66-67 20.494677746869396 27.86566185166211 27.553412716272224 24.08624768519627 68-69 19.499120155033605 28.56039274470397 28.557652789101894 23.38283431116053 70-71 22.185048828545902 27.709009216577602 27.78962300946454 22.316318945411957 72-73 21.214897148882358 26.0911094372715 30.970008974346353 21.723984439499787 74-75 19.413280245769453 30.23035032417419 28.852207596002362 21.504161834054 76-77 20.379801472008786 27.487307929144745 28.57252722162466 23.56036337722181 78-79 20.914828883974053 27.842760606914297 29.2909747300109 21.95143577910075 80-81 22.34642180364127 27.126036829007493 28.499478407897005 22.028062959454235 82-83 20.944940337522052 26.52673269857363 30.89927427380788 21.62905269009644 84-85 20.494266776846747 28.465369788568683 29.81331928138103 21.22704415320354 86-87 20.518565191569618 29.061022570283345 28.59963344310173 21.82077879504531 88-89 19.75569434972672 28.582585000490923 27.413261995033317 24.24845865474904 90-91 20.504730266253105 26.467723703061903 28.486761853872665 24.540784176812327 92-93 21.957498166097672 28.06127322444064 28.8456805447242 21.135548064737485 94-95 20.933682008980465 27.33723141284142 29.18004375561878 22.54904282255934 96-97 18.14129187390924 27.978086766888126 31.566779138419015 22.31384222078362 98-99 19.81873256532016 29.332744191989097 28.511397755584422 22.33712548710632 100-101 18.892755647416205 27.28083010990099 29.229607210552455 24.59680703213035 102-103 20.797324195453356 26.596701114643622 29.067339715268727 23.538634974634295 104-105 22.831736177930818 26.479757408663136 28.340612155264626 22.347894258141423 106-107 20.75597986586287 27.161159556465847 30.976972797738966 21.105887779932317 108-109 19.74145794754513 27.33831877435936 30.113679441160983 22.806543836934527 110-111 19.14867070414704 29.702004152760946 28.557667111383644 22.591658031708363 112-113 20.47732039096343 27.05558690407115 27.479044603661208 24.988048101304212 114-115 19.49459405817314 27.43509490927841 28.387167492940268 24.68314353960819 116-117 21.92197986264615 26.985166572013902 27.355350258702778 23.737503306637173 118-119 21.19078546259106 25.631435003224073 29.975402317020304 23.202377217164567 120-121 19.989102261546787 29.043643633310257 28.02615776498452 22.94109634015843 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4875.0 1 5725.5 2 3960.0 3 1278.0 4 1115.0 5 1060.0 6 1108.5 7 1248.5 8 1554.0 9 1673.0 10 1550.0 11 1514.0 12 1563.0 13 1655.5 14 1856.5 15 2064.0 16 2228.0 17 2376.5 18 2555.0 19 2773.5 20 3112.5 21 3603.5 22 4213.0 23 5140.5 24 6432.0 25 8106.0 26 10253.5 27 12988.0 28 16793.5 29 21209.0 30 25454.0 31 29791.5 32 34133.0 33 38346.0 34 42456.0 35 46511.5 36 50225.5 37 54478.0 38 58659.0 39 60697.5 40 62327.5 41 63644.0 42 64745.5 43 66810.5 44 67316.0 45 67776.0 46 72997.5 47 102277.5 48 168691.0 49 166280.0 50 100023.5 51 66033.5 52 48301.5 53 38760.5 54 32960.5 55 28162.0 56 23648.0 57 19054.0 58 15206.5 59 12360.5 60 9922.5 61 7692.5 62 5778.0 63 4137.5 64 2800.0 65 1893.5 66 1240.0 67 831.5 68 560.0 69 379.5 70 262.5 71 178.5 72 128.5 73 99.5 74 66.0 75 44.0 76 38.0 77 35.5 78 29.5 79 20.0 80 12.5 81 9.0 82 8.0 83 8.5 84 8.0 85 5.0 86 5.0 87 5.5 88 5.0 89 2.5 90 4.0 91 5.5 92 4.0 93 2.5 94 0.5 95 0.0 96 2.5 97 3.0 98 1.5 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20031493871975514 2 0.9495592122737564 3 0.9441310516086328 4 0.9229454148379562 5 0.9263182525327903 6 0.9372272744520193 7 0.9165159429821789 8 0.9147241229567982 9 0.9098229681814923 10-11 0.9002578112812989 12-13 0.8971748268258646 14-15 0.8982024883110094 16-17 0.9194935262596494 18-19 0.9473194372420305 20-21 0.936805669740165 22-23 0.9155146317915249 24-25 0.909190561113711 26-27 0.9133802579377628 28-29 0.9385974897655457 30-31 0.9439202492527057 32-33 0.9359361100219655 34-35 0.945026961621323 36-37 0.9545921185215167 38-39 0.9440520007251602 40-41 0.9524840949622453 42-43 0.9464498775238313 44-45 0.9291904346322974 46-47 0.9288478808039159 48-49 0.9281891234416436 50-51 0.9324051705601861 52-53 0.9289532819818794 54-55 0.9382022353481823 56-57 0.9697698881482699 58-59 0.951324682004646 60-61 0.969058430197016 62-63 0.9403629594964354 64-65 0.9345922450029301 66-67 0.9361205620834019 68-69 0.9443682042590508 70-71 0.9581494082777869 72-73 0.9626553086357292 74-75 0.9671875592881626 76-77 0.9793350450484635 78-79 1.0408893329791855 80-81 1.0080832163380258 82-83 1.0292952034031932 84-85 0.989163704893566 86-87 0.9699016396207244 88-89 0.9691901816694705 90-91 0.9774905244341011 92-93 0.9656065416187092 94-95 0.9597040756527495 96-97 0.96829427165678 98-99 1.005105633060555 100-101 0.9918777852261277 102-103 0.9644998292500918 104-105 0.9471613354750852 106-107 0.9495328619792656 108-109 0.9471613354750852 110-111 0.9506922749368646 112-113 0.9540651126316987 114-115 0.9586764141676047 116-117 0.9862915227940589 118-119 0.9842625501182601 120-121 0.9603628330150218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1897512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.49394729913346 #Duplication Level Percentage of deduplicated Percentage of total 1 83.1354868177188 46.9665181096801 2 11.001536767553022 12.43040476711239 3 2.5258131930445797 4.280794722459494 4 1.0250107086824474 2.316276038294145 5 0.5442313586267782 1.5372888846398503 6 0.3295018273878527 1.1168915322850514 7 0.23016716644123977 0.9102136239645597 8 0.15818765457795114 0.7149316016880239 9 0.13165701307539954 0.6694041922418652 >10 0.8151768061384391 8.52048944385147 >50 0.06467673965109197 2.5244286307229284 >100 0.03168347772476218 3.1800221059037845 >500 0.002481002830813069 0.9729374369174929 >1k 0.0032443883172170902 4.49816448769019 >5k 5.725391148030159E-4 2.259082238002242 >10k+ 5.725391148030159E-4 7.102152184546494 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 39690 2.091686376686946 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 32026 1.6877890627305652 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 21044 1.10903119453263 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 17262 0.9097175670035288 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10985 0.5789159699648804 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10738 0.5658989244863801 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8370 0.4411039297775192 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7695 0.405531032214816 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7548 0.397784045634494 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6902 0.3637394651522625 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5891 0.31045916969168047 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5500 0.2898532393998035 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4734 0.24948458823975816 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4568 0.24073629046878225 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4440 0.23399061507911414 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4323 0.22782464616824555 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4282 0.2256639220199925 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3790 0.19973523224095552 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3622 0.19088153329201607 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3504 0.18466286379216573 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3436 0.18107922374140453 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3144 0.16569065175872405 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3066 0.16158000581814502 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3052 0.1608421975724001 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2833 0.1493007685853897 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2649 0.13960386021274174 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2485 0.1309609636197294 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2235 0.1177858163742838 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2025 0.10671869268810948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2020 0.10645518974320056 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1969 0.10376745970512966 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.270058898178246E-5 0.0 10-11 0.0 0.0 0.0 1.0540117796356492E-4 0.0 12-13 0.0 0.0 0.0 1.0540117796356492E-4 0.0 14-15 0.0 0.0 0.0 3.1620353389069476E-4 0.0 16-17 0.0 0.0 0.0 4.743053008360421E-4 0.0 18-19 0.0 0.0 0.0 7.114579512540632E-4 0.0 20-21 0.0 0.0 0.0 0.0011330626631083228 0.0 22-23 0.0 0.0 0.0 0.001739119436398821 0.0 24-25 0.0 0.0 0.0 0.002503277976634667 0.0 26-27 0.0 0.0 0.0 0.0032937868113614038 0.0 28-29 0.0 0.0 0.0 0.005744364199014288 0.0 30-31 0.0 0.0 0.0 0.011725881048446598 0.0 32-33 0.0 0.0 0.0 0.021475490010076353 0.0 34-35 0.0 0.0 0.0 0.029275177179380156 0.0 36-37 0.0 2.635029449089123E-5 0.0 0.03354392488690454 0.0 38-39 0.0 5.270058898178246E-5 0.0 0.037312016999101985 0.0 40-41 0.0 5.270058898178246E-5 0.0 0.04271382736973468 0.0 42-43 0.0 5.270058898178246E-5 0.0 0.04943315246491195 0.0 44-45 0.0 5.270058898178246E-5 0.0 0.055124816074944454 0.0 46-47 0.0 5.270058898178246E-5 0.0 0.062239395587485084 0.0 48-49 0.0 5.270058898178246E-5 0.0 0.07088229218049741 0.0 50-51 0.0 5.270058898178246E-5 0.0 0.08295072705732559 0.0 52-53 0.0 5.270058898178246E-5 0.0 0.12089515112420895 0.0 54-55 0.0 5.270058898178246E-5 0.0 0.15493973160644042 0.0 56-57 0.0 5.270058898178246E-5 0.0 0.19723195426432086 0.0 58-59 0.0 1.0540117796356492E-4 0.0 0.28036713338308267 0.0 60-61 0.0 1.5810176694534738E-4 0.0 0.3923295346748795 0.0 62-63 0.0 1.5810176694534738E-4 0.0 0.4400499179978835 5.270058898178246E-5 64-65 0.0 1.5810176694534738E-4 0.0 0.5004184426765154 5.270058898178246E-5 66-67 0.0 1.5810176694534738E-4 0.0 0.7128808671565714 5.270058898178246E-5 68-69 0.0 1.5810176694534738E-4 0.0 0.9237095733781922 5.270058898178246E-5 70-71 0.0 1.5810176694534738E-4 0.0 1.047740409546817 5.270058898178246E-5 72-73 0.0 2.1080235592712984E-4 0.0 1.126975745080927 5.270058898178246E-5 74-75 0.0 2.1080235592712984E-4 0.0 1.430952742327848 5.270058898178246E-5 76-77 0.0 2.1080235592712984E-4 0.0 1.5253658474887115 5.270058898178246E-5 78-79 0.0 2.1080235592712984E-4 0.0 1.5891072098621772 5.270058898178246E-5 80-81 0.0 2.635029449089123E-4 0.0 1.7108719207045857 5.270058898178246E-5 82-83 0.0 2.635029449089123E-4 0.0 2.009051853163511 5.270058898178246E-5 84-85 0.0 3.1620353389069476E-4 0.0 2.2970605719489523 5.270058898178246E-5 86-87 0.0 3.42553828381586E-4 0.0 2.8294946224319 5.270058898178246E-5 88-89 0.0 3.689041228724772E-4 0.0 3.5709128585221066 5.270058898178246E-5 90-91 0.0 3.689041228724772E-4 0.0 4.333490381088499 5.270058898178246E-5 92-93 0.0 3.689041228724772E-4 0.0 4.975488956064573 5.270058898178246E-5 94-95 0.0 4.216047118542597E-4 0.0 5.528766089489816 5.270058898178246E-5 96-97 0.0 4.216047118542597E-4 0.0 6.21453250361526 5.270058898178246E-5 98-99 0.0 4.216047118542597E-4 0.0 6.833843475034676 5.270058898178246E-5 100-101 0.0 4.216047118542597E-4 0.0 7.230784311245462 5.270058898178246E-5 102-103 0.0 4.216047118542597E-4 0.0 7.9192648057034685 5.270058898178246E-5 104-105 0.0 4.216047118542597E-4 0.0 8.698785567627503 5.270058898178246E-5 106-107 0.0 4.743053008360421E-4 0.0 9.41179818625653 5.270058898178246E-5 108-109 0.0 4.743053008360421E-4 0.0 10.120621108061503 5.270058898178246E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2670 0.0 61.19946 2 ACCTGGG 2780 0.0 56.21788 3 TATAACG 360 0.0 55.93789 2 GTACCTG 3405 0.0 49.036858 1 GTACCCG 235 0.0 49.000877 1 TAACGCA 465 0.0 44.47886 4 ATAACGC 515 0.0 40.164803 3 TACCCGG 330 0.0 36.61389 2 CCTGGGG 2195 0.0 35.858337 4 GTATAAC 725 0.0 35.736847 1 TACACGG 240 0.0 31.165396 2 TCACGCA 320 0.0 28.72593 4 GTACGCA 910 0.0 27.150476 3 CCGGTCG 260 5.820766E-11 26.503542 9 GAGTACG 1130 0.0 26.495165 1 AGTACGC 940 0.0 26.319714 2 CTGGGGG 1365 0.0 26.09813 5 TACGCAG 1015 0.0 24.905241 4 TATCACG 400 0.0 24.452848 2 GTACACG 310 2.0008883E-11 24.144789 1 >>END_MODULE