##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003824_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1897512 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.851821226954034 31.0 31.0 34.0 30.0 34.0 2 32.042121999755466 33.0 31.0 34.0 30.0 34.0 3 32.10428708751249 33.0 31.0 34.0 30.0 34.0 4 35.80860358195363 37.0 35.0 37.0 35.0 37.0 5 35.6934290797634 37.0 35.0 37.0 33.0 37.0 6 35.77926411005569 37.0 35.0 37.0 33.0 37.0 7 35.73837266905295 37.0 35.0 37.0 33.0 37.0 8 35.74232995627959 37.0 35.0 37.0 33.0 37.0 9 37.358355572981885 39.0 37.0 39.0 34.0 39.0 10-11 37.25654725767215 39.0 37.0 39.0 34.0 39.0 12-13 37.23626464549368 39.0 37.0 39.0 33.0 39.0 14-15 38.372037172887445 40.0 38.0 41.0 34.0 41.0 16-17 38.41174785719405 40.0 38.0 41.0 34.0 41.0 18-19 38.44763116122586 40.0 38.0 41.0 34.0 41.0 20-21 38.386105858619075 40.0 38.0 41.0 34.0 41.0 22-23 38.30172562808562 40.0 38.0 41.0 34.0 41.0 24-25 38.17062052835503 40.0 37.5 41.0 33.0 41.0 26-27 37.97397170610779 40.0 37.0 41.0 33.0 41.0 28-29 37.84656934975905 40.0 37.0 41.0 33.0 41.0 30-31 37.67218389132717 40.0 37.0 41.0 33.0 41.0 32-33 37.411265646804864 39.0 36.0 41.0 32.0 41.0 34-35 37.34998144939267 39.0 36.0 41.0 32.0 41.0 36-37 37.19568703649832 39.0 36.0 41.0 31.5 41.0 38-39 36.98522828841135 39.0 36.0 40.5 31.0 41.0 40-41 37.05536170522242 39.0 36.0 41.0 31.0 41.0 42-43 36.93082652441724 39.0 36.0 41.0 31.0 41.0 44-45 36.69264568550818 39.0 36.0 41.0 30.0 41.0 46-47 36.52014690816185 39.0 36.0 41.0 30.0 41.0 48-49 36.26762571198496 39.0 35.0 41.0 30.0 41.0 50-51 36.01172271901311 39.0 35.0 41.0 29.0 41.0 52-53 35.87278894678927 39.0 35.0 40.5 28.0 41.0 54-55 35.706989731817245 39.0 35.0 40.0 28.0 41.0 56-57 35.404395861528144 38.5 34.5 40.0 27.5 41.0 58-59 35.14874266934807 38.0 34.0 40.0 27.0 41.0 60-61 34.73980375354675 38.0 34.0 40.0 26.0 41.0 62-63 34.53912728878658 38.0 34.0 40.0 26.0 41.0 64-65 34.11529650405373 37.0 33.5 39.5 25.5 41.0 66-67 33.71518625442158 36.5 33.0 39.0 24.5 41.0 68-69 33.384721941152414 36.0 33.0 39.0 24.5 40.5 70-71 32.914592898490234 35.5 32.0 38.5 23.5 40.0 72-73 32.47714744359983 35.0 31.5 37.5 22.5 40.0 74-75 32.11827909388715 35.0 31.0 37.0 22.0 39.5 76-77 31.145645192230667 34.0 30.5 36.0 20.5 39.0 78-79 31.56678771991956 35.0 31.0 36.0 21.0 39.0 80-81 31.31478351652058 35.0 31.0 36.0 20.0 38.5 82-83 30.996168667181024 34.5 31.0 35.5 19.5 37.0 84-85 30.666222137198606 34.0 31.0 35.0 18.5 37.0 86-87 30.448518112138423 34.0 31.0 35.0 18.0 36.0 88-89 30.123922536458267 34.0 30.5 35.0 14.5 36.0 90-91 29.803483192728162 34.0 30.0 35.0 8.5 36.0 92-93 29.574183984080207 34.0 30.0 35.0 4.5 35.5 94-95 29.222877641880526 34.0 30.0 35.0 2.0 35.0 96-97 28.846259733798785 34.0 29.0 35.0 2.0 35.0 98-99 28.5599042324897 34.0 29.0 35.0 2.0 35.0 100-101 27.64332135975952 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.006010833352501521 1101 2 0.053932177287109084 1101 3 0.002056179159374949 1101 4 -0.02554127648176774 1101 5 -0.004815319994634137 1101 6 0.025601005636971763 1101 7 0.002856090952491286 1101 8 0.016989074568733997 1101 9 -0.004420162894369639 1101 10-11 0.010761262608767197 1101 12-13 0.027244222098445903 1101 14-15 0.09410347181772494 1101 16-17 0.12185434659141237 1101 18-19 0.04104068640292269 1101 20-21 -0.020226218474668656 1101 22-23 -0.06887121213431868 1101 24-25 0.0036009028045356217 1101 26-27 0.08532069813598042 1101 28-29 0.13033578035533822 1101 30-31 0.133155724581421 1101 32-33 -0.004446407866772972 1101 34-35 0.0016800162252721407 1101 36-37 0.0414400498965648 1101 38-39 -0.04795004221566046 1101 40-41 0.01955715532889002 1101 42-43 -0.058905305685122755 1101 44-45 0.05999564670759128 1101 46-47 0.01907468834392745 1101 48-49 0.038187403584288404 1101 50-51 0.01719375757981112 1101 52-53 -0.0894955609141661 1101 54-55 0.08452162203554536 1101 56-57 -0.10760184135823891 1101 58-59 -0.2727583284028441 1101 60-61 -0.22831119685204015 1101 62-63 -0.3481234343255508 1101 64-65 -0.31669696587481155 1101 66-67 -0.2170643005377073 1101 68-69 -0.16068656186232744 1101 70-71 -0.06616045105973711 1101 72-73 -0.13455614237687996 1101 74-75 -0.24941306484474524 1101 76-77 -0.16780476286547596 1101 78-79 -0.17821467970253124 1101 80-81 -0.3606536071427229 1101 82-83 -0.15422713903563334 1101 84-85 -0.19329047327192583 1101 86-87 -0.3129790256365723 1101 88-89 -0.24479907028582204 1101 90-91 -0.10035764392926083 1101 92-93 -0.06804930999772907 1101 94-95 -0.07776774589790492 1101 96-97 -0.3327346894635532 1101 98-99 -0.2981016119690807 1101 100-101 -0.275191223952195 1102 1 -0.08244836783632081 1102 2 -0.031089996876175974 1102 3 -0.02488964754341083 1102 4 -0.09108562501977957 1102 5 0.10610835749530168 1102 6 -0.15162441385793812 1102 7 -0.1023801578734691 1102 8 -0.1429473906493115 1102 9 -0.10355476368119554 1102 10-11 -0.0445324779211731 1102 12-13 -0.08442194203560405 1102 14-15 0.014072968863636959 1102 16-17 -0.08013067131157214 1102 18-19 -0.22553559487550245 1102 20-21 -0.1591950215604001 1102 22-23 -0.03183272631229528 1102 24-25 -0.06306706072047064 1102 26-27 -0.18758812835240946 1102 28-29 -0.23795429922806477 1102 30-31 -0.14593425300125062 1102 32-33 -0.20911169366122806 1102 34-35 -0.22277011231171429 1102 36-37 -0.22588400413336984 1102 38-39 -0.2882871436273007 1102 40-41 -0.19188566019568754 1102 42-43 -0.1990751123700676 1102 44-45 -0.020903680857259133 1102 46-47 -0.2612659822474299 1102 48-49 -0.24724125109291606 1102 50-51 0.02622285189200113 1102 52-53 -0.1322209467894382 1102 54-55 -0.23046472500628568 1102 56-57 -0.09189449041272013 1102 58-59 -0.12030825991603677 1102 60-61 -0.336535682186053 1102 62-63 -0.3921065965934005 1102 64-65 -0.749842614203196 1102 66-67 -0.5510923693450636 1102 68-69 -0.6279948421478068 1102 70-71 -0.3885354018460774 1102 72-73 -0.33428896136491915 1102 74-75 -0.5259383288435231 1102 76-77 -0.29505648603076295 1102 78-79 -0.21858721917812218 1102 80-81 -0.12939571234133496 1102 82-83 -0.1543850879244708 1102 84-85 -0.25737649050294564 1102 86-87 -0.22540651610148998 1102 88-89 -0.4121705512712701 1102 90-91 -0.160501387990319 1102 92-93 -0.2204118405461415 1102 94-95 -0.10248896716632672 1102 96-97 -0.14795235656481154 1102 98-99 -0.398335088760323 1102 100-101 -0.09949158649458312 1103 1 -0.16300073231841594 1103 2 -0.09418148379365121 1103 3 -0.08928527200862035 1103 4 0.006450741004556448 1103 5 0.022134210263494936 1103 6 -0.024570887624683735 1103 7 -0.010485133730909979 1103 8 0.024465630208098332 1103 9 -0.05353035619683766 1103 10-11 -0.058879118312681555 1103 12-13 -0.14998777291033605 1103 14-15 -0.1258443726258136 1103 16-17 -0.12263994108247545 1103 18-19 -0.16127266144536634 1103 20-21 -0.07689095503676668 1103 22-23 -0.07540465173023136 1103 24-25 0.0026629147142074316 1103 26-27 -0.20194638061870052 1103 28-29 -0.24136176442343782 1103 30-31 -0.3029593808727995 1103 32-33 -0.1418081400893385 1103 34-35 -0.1818741039782239 1103 36-37 -0.3403137314346907 1103 38-39 -0.1654370033265451 1103 40-41 -0.1271711037523886 1103 42-43 -0.18641805612688245 1103 44-45 -0.10527240635817492 1103 46-47 -0.14402584304021815 1103 48-49 -0.07978820872536119 1103 50-51 -0.011799048329301343 1103 52-53 -0.08179305484075172 1103 54-55 -0.10995781594079546 1103 56-57 -0.1848529593588495 1103 58-59 -0.19555613962152307 1103 60-61 -0.33446121512037763 1103 62-63 -0.2776427100876333 1103 64-65 -0.15733396448385406 1103 66-67 -0.2251384763794917 1103 68-69 -0.2975408403913704 1103 70-71 -0.3340327564806316 1103 72-73 -0.21695615263278967 1103 74-75 -0.39144294371821786 1103 76-77 -0.26565668467273795 1103 78-79 -0.11814754624969126 1103 80-81 -0.08222861520856739 1103 82-83 -0.1318538796018025 1103 84-85 -0.42918849537767656 1103 86-87 -0.36570795135176226 1103 88-89 -0.4987175742909642 1103 90-91 -0.3791169143069979 1103 92-93 -0.3776413599610855 1103 94-95 -0.40932452836372235 1103 96-97 -0.35531958379567286 1103 98-99 -0.3192529229089267 1103 100-101 -0.26200597450932506 1104 1 -0.013291676703726552 1104 2 0.0845040852710639 1104 3 0.06401874025746679 1104 4 0.008361455541454177 1104 5 -5.399929802649694E-4 1104 6 0.05344229747784368 1104 7 -0.11182099280223667 1104 8 -0.05656695274974055 1104 9 0.029881828300823088 1104 10-11 -0.06662466271394152 1104 12-13 0.009987915298559358 1104 14-15 -0.02072729154118491 1104 16-17 -0.030168859688380678 1104 18-19 0.019409897364788264 1104 20-21 -0.13841924533408445 1104 22-23 -0.08575838008523817 1104 24-25 0.04389507942482851 1104 26-27 0.04274265352096762 1104 28-29 0.020155858694565154 1104 30-31 -0.13523767937564202 1104 32-33 0.11442993259752399 1104 34-35 -0.007880218264737948 1104 36-37 0.10792219843857254 1104 38-39 0.09911584258311734 1104 40-41 0.0954700429576647 1104 42-43 -0.04520077857859661 1104 44-45 -0.00594455587686582 1104 46-47 0.006830227405366429 1104 48-49 -0.04047566740469222 1104 50-51 0.11705098285168702 1104 52-53 0.10464860858278513 1104 54-55 0.01970092809848012 1104 56-57 -0.011011461921313526 1104 58-59 -0.05000974559701632 1104 60-61 -0.08781879772654122 1104 62-63 -0.3685432544654432 1104 64-65 -0.2683111953309876 1104 66-67 -0.1716329599239046 1104 68-69 -0.22810441912452717 1104 70-71 -0.13689835939897677 1104 72-73 -0.14985220976198121 1104 74-75 -0.06490300157887674 1104 76-77 -0.2208356588417537 1104 78-79 -0.2269456247507513 1104 80-81 -0.1157879130033912 1104 82-83 -0.12802025946591655 1104 84-85 -0.10921695681875221 1104 86-87 -0.2853380229508069 1104 88-89 -0.4570802912098131 1104 90-91 -0.3317364687705684 1104 92-93 -0.3531155219427866 1104 94-95 -0.20251710963356118 1104 96-97 -0.21117082470512472 1104 98-99 -0.21438822025362114 1104 100-101 0.028592020529845996 1105 1 -0.07581802430917861 1105 2 -0.06658203262303886 1105 3 -0.00920473516071496 1105 4 -0.01400716436102556 1105 5 -0.12140689879702649 1105 6 0.018139488922521707 1105 7 -0.027151719876833624 1105 8 -0.02726443849220317 1105 9 -0.0414237402904476 1105 10-11 -0.049959553704447046 1105 12-13 -0.08674110405350888 1105 14-15 2.8517236756187003E-4 1105 16-17 0.05134416895394622 1105 18-19 9.193467097716734E-4 1105 20-21 -0.12762965233996226 1105 22-23 -0.05134940161669732 1105 24-25 -0.044634366261320224 1105 26-27 -0.07992931183143526 1105 28-29 -0.12903472298102514 1105 30-31 -0.021944964438837644 1105 32-33 0.1287640242116268 1105 34-35 0.029736705448506484 1105 36-37 -0.07815602511638531 1105 38-39 -0.12112627699722367 1105 40-41 0.1918983019078908 1105 42-43 0.3121811244940318 1105 44-45 0.2699939160017877 1105 46-47 0.1662655534793558 1105 48-49 0.0997244153168495 1105 50-51 0.28103102230350174 1105 52-53 0.260529493544162 1105 54-55 0.25923297474070495 1105 56-57 0.20662025622094404 1105 58-59 0.3054865637497173 1105 60-61 0.28456036121811934 1105 62-63 0.17026825973641024 1105 64-65 0.0911606196909176 1105 66-67 0.02351082840440455 1105 68-69 -0.05852576207689708 1105 70-71 0.14409040160735742 1105 72-73 0.20306899399548684 1105 74-75 0.09937829539939713 1105 76-77 0.09889949303976309 1105 78-79 0.14115875949079637 1105 80-81 -0.001487526439277076 1105 82-83 0.15151540624361814 1105 84-85 0.11717708007187255 1105 86-87 0.13419639000465722 1105 88-89 0.07827024131008642 1105 90-91 0.006159143010890489 1105 92-93 0.08061378773006211 1105 94-95 0.05417070802907986 1105 96-97 0.1302096307405769 1105 98-99 0.46626687070030215 1105 100-101 0.566754787088076 1106 1 0.11077224612543546 1106 2 0.048723779586197224 1106 3 0.05361788280372082 1106 4 -0.01911036857638493 1106 5 -0.014550910761691682 1106 6 0.005350739556860162 1106 7 -0.0388506380936704 1106 8 6.564766529848498E-4 1106 9 0.06529084913942285 1106 10-11 -0.011758037777262587 1106 12-13 -0.08485583588797851 1106 14-15 0.06103822892476529 1106 16-17 -0.007454037910534339 1106 18-19 -0.003859003990065446 1106 20-21 -0.023868784767309137 1106 22-23 -0.03290331081309006 1106 24-25 0.09911142810822327 1106 26-27 0.17706111665663826 1106 28-29 0.031474351120785116 1106 30-31 0.07852489250824846 1106 32-33 0.014604211371967324 1106 34-35 0.1868279014012657 1106 36-37 0.11781196290999674 1106 38-39 0.1141660324835243 1106 40-41 0.14380433275402993 1106 42-43 0.15700789164126405 1106 44-45 0.3831107045432489 1106 46-47 0.3487218708113602 1106 48-49 0.4084517831566572 1106 50-51 0.41541768209956587 1106 52-53 0.3623528369449289 1106 54-55 0.2665576181144189 1106 56-57 0.21208408276901025 1106 58-59 0.4241634472573921 1106 60-61 0.25851172009784307 1106 62-63 0.300061560684064 1106 64-65 0.25933162593481285 1106 66-67 0.16700065217592197 1106 68-69 0.030083238922394173 1106 70-71 0.037246709102937814 1106 72-73 0.1036796068271002 1106 74-75 0.19418005231899116 1106 76-77 0.11667813212568845 1106 78-79 0.005745422863750349 1106 80-81 0.2011451982280157 1106 82-83 0.18616211651843528 1106 84-85 0.10928376839357767 1106 86-87 0.25807721724797617 1106 88-89 0.18431807763198194 1106 90-91 0.07656363244455378 1106 92-93 0.21959799803282465 1106 94-95 0.30993829007939055 1106 96-97 0.2839308731704442 1106 98-99 0.3544434881773917 1106 100-101 0.4134821918144773 1107 1 -0.012364142610071127 1107 2 -0.023059765416562072 1107 3 0.025456645918026766 1107 4 -0.05721086553938903 1107 5 0.03238448404780314 1107 6 0.03516851978877611 1107 7 0.00974130006204632 1107 8 -0.009282783424318097 1107 9 0.10007825940999027 1107 10-11 0.0487057943596767 1107 12-13 -0.09451900540758373 1107 14-15 0.1441414867933517 1107 16-17 0.08862547617008687 1107 18-19 0.08752697060960202 1107 20-21 0.07027809540939955 1107 22-23 0.1128725036540601 1107 24-25 0.1634405280530018 1107 26-27 0.1254321823661897 1107 28-29 0.021811181601187002 1107 30-31 -0.008995814283572656 1107 32-33 0.004941041852362105 1107 34-35 0.15618184949624236 1107 36-37 0.17827579504694313 1107 38-39 0.2527634924503914 1107 40-41 0.33182200254972116 1107 42-43 0.2878748159924527 1107 44-45 0.4515811628535715 1107 46-47 0.4735148028930851 1107 48-49 0.2811740360556101 1107 50-51 0.34170150319288695 1107 52-53 0.2764886734993226 1107 54-55 0.33696071032866115 1107 56-57 0.3606208028131874 1107 58-59 0.4321700734307754 1107 60-61 0.1386884180547696 1107 62-63 0.2216518422964313 1107 64-65 0.15027585564215684 1107 66-67 0.1473982225790067 1107 68-69 0.15349571821560204 1107 70-71 0.02427045289089591 1107 72-73 0.03990268799772423 1107 74-75 0.15083383475963075 1107 76-77 0.06753401285456917 1107 78-79 0.028384848595390366 1107 80-81 0.05178020651073467 1107 82-83 0.16407487190219783 1107 84-85 0.24125505497557498 1107 86-87 0.2892754502684092 1107 88-89 0.3516289555999563 1107 90-91 0.3131732403959617 1107 92-93 0.2574224044381275 1107 94-95 0.36267158660065135 1107 96-97 0.3849800172897133 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-1.0609601802687791 2316 66-67 -0.9177855466953488 2316 68-69 -0.7951476411284588 2316 70-71 -0.9137483921429244 2316 72-73 -1.1263584311110328 2316 74-75 -0.9195198974531387 2316 76-77 -0.4442312056655915 2316 78-79 -0.5174662534344421 2316 80-81 -0.6157060531474627 2316 82-83 -0.7416417394921133 2316 84-85 -0.7397015671658416 2316 86-87 -0.8104689987202924 2316 88-89 -0.8140711229059505 2316 90-91 -0.8306722906435198 2316 92-93 -0.9921135573062436 2316 94-95 -1.1865544377278638 2316 96-97 -1.4248250486736893 2316 98-99 -1.4409763014585977 2316 100-101 -1.663041902194447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 15.0 5 30.0 6 63.0 7 180.0 8 485.0 9 902.0 10 1563.0 11 2931.0 12 4598.0 13 6758.0 14 7589.0 15 9724.0 16 12229.0 17 16179.0 18 14429.0 19 10259.0 20 9054.0 21 9202.0 22 9509.0 23 10578.0 24 12128.0 25 14698.0 26 18112.0 27 22755.0 28 29315.0 29 36400.0 30 46250.0 31 57540.0 32 72977.0 33 94164.0 34 125446.0 35 173973.0 36 259634.0 37 374739.0 38 359304.0 39 73766.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.99265601486513 19.962109508056344 14.199337944591086 19.84589653248743 2 14.970044101154386 24.030702161209536 37.974477405536675 23.0247763320994 3 23.310946352964248 26.3578883659578 29.687202622118352 20.643962658959598 4 12.369671443448052 17.193250951772637 41.072572927075036 29.36450467770428 5 11.73510365151841 40.22551636037085 34.40394579849824 13.635434189612502 6 27.31476796984683 39.454981048868206 17.661179481341883 15.569071499943082 7 26.953189228842824 33.1312265745882 22.703729936885775 17.211854259683207 8 24.48290182091075 32.0492834827922 19.943483888375937 23.52433080792111 9 23.300406005337514 21.134464498775237 23.8231958480368 31.741933647850452 10-11 25.03549384667923 25.822392691060713 28.38627634502443 20.75583711723562 12-13 28.555234433300026 23.84717461602351 27.28957708831354 20.308013862362927 14-15 22.37431527821549 26.940514976131574 29.332153175306697 21.353016570346238 16-17 19.951889632318533 29.468904544477187 29.34927420748854 21.22993161571574 18-19 19.441431727440985 31.872894611470176 29.41694176374115 19.268731897347685 20-21 22.001511616997995 27.423831474733124 28.28165303857153 22.29300386969735 22-23 20.230443926163293 28.207549730265143 29.011892681546332 22.550113662025236 24-25 23.165202644304753 28.073788202656953 28.66619552340117 20.094813629637127 26-27 22.286242375116345 25.906184548799782 32.1435949923211 19.66397808376278 28-29 19.95504574486813 30.91270472923613 29.722700396676892 19.409549129218842 30-31 21.5065820663891 27.621962515966647 28.857234791596227 22.01422062604803 32-33 21.706134585850368 28.290940368060397 29.74368690728289 20.25923813880635 34-35 23.457866643055457 26.6342065263908 29.895899871614056 20.01202695893969 36-37 22.633325769274055 26.212469944724386 31.71646953990226 19.437734746099306 38-39 21.21742151718073 28.67214920316404 31.036875312716 19.07355396693923 40-41 20.670123277469962 29.28837314953355 29.404609505398625 20.636894067597858 42-43 19.99033883390832 29.608364366688956 27.74617855553181 22.655118243870916 44-45 21.99587467160631 27.013838323705198 28.073842693064133 22.916444311624364 46-47 23.39616426516006 29.103967492700615 28.2243520148412 19.27551622729812 48-49 22.812840582581988 28.42408684342002 28.62833098987923 20.13474158411876 50-51 19.67682246527088 29.109324991001177 31.193941830995048 20.019910712732898 52-53 21.539008413463122 30.542565472521794 27.30450602096607 20.613920093049018 54-55 20.24296256009242 28.701908938842497 28.310919560219954 22.744208940845137 56-57 22.866072757985943 27.123820868146737 28.485427452179195 21.52467892168812 58-59 25.182180430897926 26.976749579086977 27.414119645497554 20.426950344517536 60-61 21.955342564806354 28.55772200385576 30.612404620585192 18.874530810752695 62-63 20.77145181364381 28.477089421129175 29.796678049730435 20.954780715496575 64-65 20.151847209810157 31.721125785256337 27.951341597417667 20.175685407515836 66-67 21.83144683398393 27.854335767800976 27.180852732324627 23.13336466589047 68-69 20.657363120075 29.25559239406469 27.677273574295665 22.409770911564646 70-71 23.4064444308572 28.775635092483466 27.137672069002594 20.68024840765674 72-73 22.471692500090253 26.716019076584335 30.337476571263544 20.474811852061876 74-75 20.376710072742814 31.516194670164587 27.893915542867564 20.21317971422503 76-77 21.74410151048868 27.952512215424676 27.543526384869345 22.759859889217303 78-79 21.825361586948326 28.892226022244493 28.323715871933835 20.958696518873353 80-81 23.393226237919617 27.82110969286924 28.02487802358685 20.76078604562429 82-83 22.072554451939105 27.175745547164983 30.229844653409565 20.521855347486348 84-85 20.82834430217508 29.201668936324506 29.32912638551017 20.640860375990254 86-87 20.990510351864305 29.637705830394278 27.550573510779806 21.821210306961607 88-89 20.53267176457568 29.59763057902198 26.554616312957506 23.31508134344483 90-91 21.96222181115438 26.821122773843648 27.59611741151633 23.620538003485645 92-93 22.791434750220617 29.07948307895976 27.85013158071562 20.278950590104003 94-95 21.91585211668807 28.44747397717697 28.15295006137123 21.483723844763738 96-97 19.054993738853263 29.332734624352273 30.685469080054133 20.926802556740327 98-99 21.07831265770247 30.44015047203684 27.04572795130082 21.43580891895987 100-101 19.321254304715342 29.227343783764407 27.540796631912713 23.910605279607534 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 382.0 1 272.5 2 428.0 3 603.0 4 427.5 5 356.5 6 458.0 7 776.0 8 1193.0 9 1424.0 10 1397.5 11 1370.0 12 1456.0 13 1611.0 14 1832.0 15 2043.0 16 2299.5 17 2572.0 18 2751.5 19 2955.0 20 3223.0 21 3689.0 22 4402.5 23 5364.5 24 6525.0 25 7983.5 26 10093.0 27 12775.0 28 16439.5 29 20929.5 30 25416.0 31 29622.5 32 33935.5 33 38108.5 34 42072.5 35 46477.5 36 50147.5 37 54354.5 38 58540.0 39 60645.5 40 62074.0 41 63091.5 42 64675.5 43 67238.0 44 67529.5 45 67544.0 46 71909.5 47 104363.0 48 181088.0 49 176930.0 50 101838.5 51 66876.5 52 48477.5 53 38387.0 54 32526.5 55 27617.0 56 22693.5 57 18126.0 58 14624.0 59 11764.0 60 9209.5 61 7008.5 62 5156.0 63 3558.5 64 2292.5 65 1488.5 66 943.5 67 548.0 68 338.0 69 200.5 70 94.5 71 51.0 72 36.0 73 15.0 74 11.0 75 10.0 76 6.0 77 3.0 78 1.5 79 1.5 80 2.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05238438544789177 2 0.002951232982979818 3 0.038207927011792285 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001370215313526344 16-17 0.0 18-19 0.0 20-21 0.010856321330247188 22-23 2.635029449089123E-5 24-25 0.0 26-27 0.00558626243206894 28-29 0.013175147245445613 30-31 0.010408366323902036 32-33 0.00558626243206894 34-35 0.005691663610032506 36-37 0.02015797528553179 38-39 0.04337258473200697 40-41 0.004479550063451509 42-43 0.011883982815391944 44-45 0.010988072802701643 46-47 0.004848454186323986 48-49 0.001475616491489909 50-51 0.002160724148253081 52-53 9.222603071811931E-4 54-55 9.486106016720842E-4 56-57 0.010250264556956688 58-59 0.007826037463794695 60-61 0.004743053008360421 62-63 0.005401810370632702 64-65 0.07354367192407743 66-67 0.03459793666654019 68-69 0.026007740662509642 70-71 0.016231781406389 72-73 1.3175147245445614E-4 74-75 2.635029449089123E-4 76-77 7.114579512540632E-4 78-79 4.4795500634515096E-4 80-81 7.378082457449545E-4 82-83 6.85107656763172E-4 84-85 8.432094237085194E-4 86-87 0.004005244762615467 88-89 1.0540117796356492E-4 90-91 7.905088347267369E-4 92-93 3.9525441736336847E-4 94-95 0.002266125326216646 96-97 0.005059256542251116 98-99 0.007878738052776477 100-101 0.009169902482830148 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1897512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.42011666005904 #Duplication Level Percentage of deduplicated Percentage of total 1 81.50232758423356 43.53863847616104 2 11.926787657518306 12.742607760887603 3 2.8300593894201485 4.535463082131593 4 1.1211985247260114 2.395782239597984 5 0.6006521088076447 1.6043452862307426 6 0.37867998800159736 1.2137477481525052 7 0.26587931701308326 0.9942312892635009 8 0.18389558301297845 0.7858978798258304 9 0.15055081456071853 0.7238197869390449 >10 0.9192327653292467 9.293139660731352 >50 0.07392824602438068 2.6613035617608136 >100 0.039637901243881044 3.823499575997392 >500 0.0025892100391567423 0.9615083150225088 >1k 0.0031867200481929137 4.059465337099568 >5k 4.979250075301428E-4 1.864498363666531 >10k+ 8.962650135542569E-4 8.802051636531992 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 57600 3.0355539253506696 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 22788 1.2009410217168588 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 16490 0.8690327123095927 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14751 0.777386388070273 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12005 0.6326705707262984 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11320 0.5965706672737775 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10235 0.5393905282285435 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10166 0.5357541875888004 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10102 0.5323813498939665 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8999 0.4742526002470604 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8051 0.4242924418923306 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7134 0.37596600179603606 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5455 0.28748171289562335 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5409 0.2850574858024613 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4803 0.25312092887950116 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4496 0.23694184806209395 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4362 0.22987996913853512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4352 0.2293529632487173 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4281 0.22561122143101073 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4168 0.2196560548760693 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3500 0.1844520614362386 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3353 0.17670507485591658 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3303 0.17407004540682747 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3285 0.17312143480515538 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2760 0.1454536255897196 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2619 0.13802284254328825 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2552 0.13449190308150882 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2358 0.12426798881904305 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2254 0.11878712756493767 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2197 0.11578319399297606 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1982 0.10445256736189283 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1961 0.1033458549932754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.270058898178246E-5 0.0 10-11 0.0 0.0 0.0 1.3175147245445614E-4 0.0 12-13 0.0 0.0 0.0 1.5810176694534738E-4 0.0 14-15 0.0 0.0 0.0 4.216047118542597E-4 0.0 16-17 0.0 0.0 0.0 5.270058898178246E-4 0.0 18-19 0.0 0.0 0.0 7.378082457449545E-4 0.0 20-21 0.0 0.0 0.0 0.001054011779635649 0.0 22-23 0.0 2.635029449089123E-5 0.0 0.0016337182584352564 0.0 24-25 0.0 5.270058898178246E-5 0.0 0.0023978767986711017 0.0 26-27 0.0 5.270058898178246E-5 0.0 0.0032674365168705124 0.0 28-29 0.0 5.270058898178246E-5 0.0 0.005876115671468745 0.0 30-31 0.0 5.270058898178246E-5 0.0 0.012252886938264422 0.0 32-33 0.0 5.270058898178246E-5 0.0 0.022503151495221113 0.0 34-35 0.0 5.270058898178246E-5 0.0 0.030513641020452044 0.0 36-37 0.0 7.905088347267369E-5 0.0 0.03488778990593999 0.0 38-39 0.0 1.0540117796356492E-4 0.0 0.03881398378508279 0.0 40-41 0.0 1.0540117796356492E-4 0.0 0.04490090181247866 0.0 42-43 0.0 1.5810176694534738E-4 0.0 0.051883729852564835 0.0 44-45 0.0 1.5810176694534738E-4 0.0 0.05783889640750625 0.0 46-47 0.0 1.5810176694534738E-4 0.0 0.06511157768699223 0.0 48-49 0.0 1.5810176694534738E-4 0.0 0.07417607899185881 0.0 50-51 0.0 1.5810176694534738E-4 0.0 0.08653436710808679 0.0 52-53 0.0 1.5810176694534738E-4 0.0 0.126217910611369 0.0 54-55 0.0 1.5810176694534738E-4 0.0 0.16171175729059947 0.0 56-57 0.0 1.5810176694534738E-4 0.0 0.20605930291876942 0.0 58-59 0.0 1.5810176694534738E-4 0.0 0.29314702621116495 0.0 60-61 0.0 1.5810176694534738E-4 0.0 0.40972072903886775 0.0 62-63 0.0 1.5810176694534738E-4 0.0 0.4585741750249801 0.0 64-65 0.0 1.5810176694534738E-4 0.0 0.5205764179620471 0.0 66-67 0.0 1.5810176694534738E-4 0.0 0.7404959757830254 0.0 68-69 0.0 1.5810176694534738E-4 0.0 0.9596250247692768 0.0 70-71 0.0 1.5810176694534738E-4 0.0 1.0892421233699707 0.0 72-73 0.0 1.5810176694534738E-4 0.0 1.1721137995438236 0.0 74-75 0.0 1.5810176694534738E-4 0.0 1.4888706896188273 0.0 76-77 0.0 1.5810176694534738E-4 0.0 1.5896078654575043 0.0 78-79 0.0 1.5810176694534738E-4 0.0 1.6564585625808954 0.0 80-81 0.0 1.5810176694534738E-4 0.0 1.7817542128850832 0.0 82-83 0.0 1.5810176694534738E-4 0.0 2.090948568441201 0.0 84-85 0.0 1.5810176694534738E-4 0.0 2.3898136085568895 0.0 86-87 0.0 1.5810176694534738E-4 0.0 2.9425373857978236 0.0 88-89 0.0 1.5810176694534738E-4 0.0 3.710305916378921 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 345 0.0 56.45606 2 ATAACGC 395 0.0 52.917747 3 TACCTGG 2800 0.0 50.559647 2 TAACGCA 420 0.0 48.61885 4 ACCTGGG 2935 0.0 46.9392 3 GTACCTG 3125 0.0 46.244026 1 GTACCGG 175 0.0 43.462433 1 TACCGGG 235 0.0 34.36583 2 GTACCCG 250 0.0 34.22666 1 TACACGG 260 0.0 32.888565 2 CCTGGGG 1895 0.0 32.82824 4 GTATAAC 660 0.0 32.411617 1 ACACGGG 280 0.0 32.236015 3 GTACACG 310 0.0 30.669054 1 TACCCGG 300 0.0 28.503426 2 TACGCAG 875 0.0 27.136103 4 GAGTACG 930 0.0 27.090998 1 AGTACGC 915 0.0 25.440214 2 GTACGCA 925 0.0 25.165186 3 ACGCAGT 955 0.0 24.365665 5 >>END_MODULE