##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003823_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 519174 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97169735002138 31.0 31.0 34.0 28.0 34.0 2 30.77580541398452 33.0 31.0 34.0 27.0 34.0 3 30.887633047879902 33.0 31.0 34.0 27.0 34.0 4 34.350304522183315 37.0 35.0 37.0 32.0 37.0 5 34.34550844225635 37.0 35.0 37.0 30.0 37.0 6 34.439008887193886 37.0 35.0 37.0 31.0 37.0 7 34.3509883006468 37.0 35.0 37.0 30.0 37.0 8 34.3560174430923 37.0 35.0 37.0 30.0 37.0 9 35.843482146640625 39.0 37.0 39.0 30.0 39.0 10-11 35.717412659339644 39.0 37.0 39.0 30.0 39.0 12-13 35.661122282702905 39.0 36.0 39.0 30.0 39.0 14-15 36.73800209563653 40.0 37.0 41.0 30.5 41.0 16-17 36.75821882451741 40.0 37.0 41.0 31.0 41.0 18-19 36.7065145789273 40.0 37.0 41.0 30.0 41.0 20-21 36.60948159961785 40.0 37.0 41.0 30.0 41.0 22-23 36.42876665626553 40.0 36.0 41.0 29.0 41.0 24-25 36.31801188041004 39.5 36.0 41.0 29.0 41.0 26-27 36.13536116985827 39.0 36.0 41.0 28.0 41.0 28-29 36.013114100474986 39.0 36.0 41.0 28.0 41.0 30-31 35.74935763347163 39.0 35.5 40.5 27.0 41.0 32-33 35.42320590014138 39.0 35.0 40.0 26.5 41.0 34-35 35.104935917438084 39.0 35.0 40.0 24.5 41.0 36-37 35.17626845720317 38.5 35.0 40.0 25.0 41.0 38-39 35.12351831948441 38.5 35.0 40.0 25.0 41.0 40-41 35.255381625428086 39.0 35.0 41.0 25.0 41.0 42-43 35.16964639985824 39.0 35.0 41.0 24.0 41.0 44-45 34.91235886234672 39.0 35.0 41.0 23.5 41.0 46-47 34.56663758200526 39.0 34.5 41.0 21.5 41.0 48-49 34.25025328695197 39.0 34.0 41.0 18.0 41.0 50-51 33.49660711052557 38.0 33.5 39.5 15.0 40.5 52-53 33.65654096699758 38.0 33.5 40.0 15.0 40.5 54-55 33.94801261234191 38.0 34.0 40.0 14.0 41.0 56-57 33.70950394280145 38.0 34.0 40.0 11.0 41.0 58-59 33.45678038576662 38.0 33.0 40.0 8.0 41.0 60-61 33.06256091406735 38.0 33.0 40.0 7.5 41.0 62-63 32.85984178714651 38.0 32.5 40.0 7.0 41.0 64-65 32.5829750719412 37.0 32.0 40.0 4.0 41.0 66-67 32.21698987237419 36.5 32.0 39.5 2.0 41.0 68-69 31.858059147800162 36.0 31.0 39.0 2.0 41.0 70-71 31.35509193449595 35.5 31.0 39.0 2.0 40.5 72-73 30.949835700555113 35.0 30.5 38.0 2.0 40.0 74-75 30.57452607411003 35.0 30.0 37.0 2.0 40.0 76-77 30.148042852685226 35.0 30.0 37.0 2.0 39.0 78-79 29.50557038680674 34.5 29.0 36.0 2.0 39.0 80-81 29.162839433407683 34.0 29.0 36.0 2.0 38.5 82-83 28.803333757083365 34.0 28.5 35.5 2.0 37.0 84-85 28.557049274424372 34.0 28.5 35.0 2.0 37.0 86-87 28.376204316857162 34.0 29.0 35.0 2.0 36.5 88-89 28.03926429289602 34.0 27.5 35.0 2.0 36.0 90-91 27.75918285584409 34.0 27.0 35.0 2.0 36.0 92-93 27.50532865667387 34.0 26.0 35.0 2.0 35.5 94-95 27.196144259920565 34.0 25.5 35.0 2.0 35.0 96-97 26.92570698840851 34.0 25.0 35.0 2.0 35.0 98-99 26.669462453820877 34.0 25.0 35.0 2.0 35.0 100-101 26.336397816531644 33.0 24.5 35.0 2.0 35.0 102-103 25.992105729485683 33.0 23.5 35.0 2.0 35.0 104-105 25.6130988839965 33.0 20.0 35.0 2.0 35.0 106-107 25.21360372437757 32.0 18.5 35.0 2.0 35.0 108-109 24.945681024088262 32.0 18.0 35.0 2.0 35.0 110-111 24.62219891597037 32.0 13.0 35.0 2.0 35.0 112-113 24.111927792994255 32.0 4.5 34.5 2.0 35.0 114-115 23.58130318544457 31.5 2.0 34.0 2.0 35.0 116-117 22.8910846845181 31.0 2.0 34.0 2.0 35.0 118-119 22.12404608089003 31.0 2.0 34.0 2.0 35.0 120-121 20.635347686902655 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7902876449250336 1101 2 0.8912741274773239 1101 3 0.9054098496362286 1101 4 0.9593176666749628 1101 5 1.1174221346822293 1101 6 1.072200974959408 1101 7 1.0103153332880979 1101 8 0.9404205611654959 1101 9 1.1456388968238684 1101 10-11 1.1500717583967557 1101 12-13 0.8478272597095113 1101 14-15 1.082114477335388 1101 16-17 1.0992727206303243 1101 18-19 1.2228543363609887 1101 20-21 1.2535026334326957 1101 22-23 1.0115328013684888 1101 24-25 1.1098223922004777 1101 26-27 0.9318482674426747 1101 28-29 1.0748825102475479 1101 30-31 1.0571883626926848 1101 32-33 1.3482743489766449 1101 34-35 1.4877113282979266 1101 36-37 1.1985958156456817 1101 38-39 1.1735270627660626 1101 40-41 1.2923334598401155 1101 42-43 1.386128767388385 1101 44-45 1.3778367686608632 1101 46-47 1.5885791904234523 1101 48-49 1.8402326635472406 1101 50-51 1.2022655301518412 1101 52-53 1.3481303865980792 1101 54-55 1.429581426022196 1101 56-57 1.553732202426481 1101 58-59 1.6760135788094672 1101 60-61 1.7321798869316822 1101 62-63 1.7605741712285266 1101 64-65 1.8743622477197803 1101 66-67 1.7276250746251272 1101 68-69 1.6271268403707175 1101 70-71 1.2267884868412722 1101 72-73 1.3598805738497113 1101 74-75 1.6367763061764613 1101 76-77 1.7102342508981856 1101 78-79 1.797964664000574 1101 80-81 1.9259149079186209 1101 82-83 1.9395553197710207 1101 84-85 1.7484944071781783 1101 86-87 1.7993526577677699 1101 88-89 1.9579561447447347 1101 90-91 2.079176743508075 1101 92-93 2.0773943032549766 1101 94-95 2.2345759259412183 1101 96-97 2.226322834391425 1101 98-99 2.018803447850722 1101 100-101 2.2500281286198778 1101 102-103 2.430352215898722 1101 104-105 2.4629776503417027 1101 106-107 2.488245558204472 1101 108-109 2.3297542237570603 1101 110-111 2.2969470874529243 1101 112-113 2.4311342967096294 1101 114-115 2.4999509569448257 1101 116-117 2.8827773037763045 1101 118-119 2.9921216652947074 1101 120-121 2.9950860298191238 1102 1 0.8354397620688516 1102 2 0.8874916253545742 1102 3 0.879239132386676 1102 4 1.225356052422896 1102 5 1.3324968715411956 1102 6 1.307823141286967 1102 7 1.1696113809658826 1102 8 1.0499769462405553 1102 9 1.4096098031184283 1102 10-11 1.3768476599651862 1102 12-13 1.2752147539019845 1102 14-15 1.4798423326358616 1102 16-17 1.4837429941047446 1102 18-19 1.4965594581988881 1102 20-21 1.4513939005148373 1102 22-23 1.5242729125525756 1102 24-25 1.4462720309322705 1102 26-27 1.4951330845656727 1102 28-29 1.5320189590852493 1102 30-31 1.6169133662973678 1102 32-33 1.5289821462819475 1102 34-35 1.45881267564409 1102 36-37 1.3783780133739398 1102 38-39 1.5783060563417308 1102 40-41 1.3762882652679451 1102 42-43 1.7011606487065762 1102 44-45 1.660392961486771 1102 46-47 1.6299279784527414 1102 48-49 1.8355842391892736 1102 50-51 1.543286293060575 1102 52-53 1.8222424358138625 1102 54-55 1.8300780696723677 1102 56-57 2.165797438973044 1102 58-59 2.0889451381103257 1102 60-61 2.1429021830274237 1102 62-63 2.0034184974753444 1102 64-65 1.9324363117067094 1102 66-67 2.1633589857739715 1102 68-69 2.1263706603614274 1102 70-71 1.4992435886532292 1102 72-73 1.5299882333764536 1102 74-75 1.722938372214042 1102 76-77 1.8077918855927457 1102 78-79 1.960647500957272 1102 80-81 1.938540470647915 1102 82-83 2.2449767459393364 1102 84-85 1.9899269036936644 1102 86-87 2.1898215053942955 1102 88-89 2.4767252695304585 1102 90-91 2.2099814664289816 1102 92-93 2.217897195002685 1102 94-95 2.1844733930585996 1102 96-97 2.2086455245979337 1102 98-99 2.5671661258814495 1102 100-101 2.623123660429105 1102 102-103 2.715847017161156 1102 104-105 2.6951693708114313 1102 106-107 2.5530727742765045 1102 108-109 2.391115347773784 1102 110-111 2.4832998005691245 1102 112-113 2.6535990109793204 1102 114-115 2.576512981328431 1102 116-117 2.451839208004184 1102 118-119 2.491608199843487 1102 120-121 2.315297824304775 1103 1 0.7473293827819987 1103 2 1.0658142566183813 1103 3 0.9582689261223507 1103 4 1.1498961387276907 1103 5 1.065747960674642 1103 6 1.2370222410742997 1103 7 1.3735611672686119 1103 8 1.1237076670300965 1103 9 1.3579095161240176 1103 10-11 1.1119440818263158 1103 12-13 1.0698589014873008 1103 14-15 1.283132960926487 1103 16-17 1.1298427607927408 1103 18-19 1.3166036429489552 1103 20-21 1.2934143204764368 1103 22-23 1.3472329811008876 1103 24-25 1.3773551767213021 1103 26-27 1.5401514604663973 1103 28-29 1.473304859194677 1103 30-31 1.3866999787310306 1103 32-33 1.3428735013497715 1103 34-35 1.4400067994852677 1103 36-37 1.1370141705395085 1103 38-39 1.307145896946281 1103 40-41 1.5094035998538686 1103 42-43 1.5344989717507787 1103 44-45 1.5130177214644718 1103 46-47 1.535755616633324 1103 48-49 1.5357504481790087 1103 50-51 1.6191204350711885 1103 52-53 1.6177289842415874 1103 54-55 1.671902858555974 1103 56-57 1.5950440270431656 1103 58-59 1.389795281607526 1103 60-61 1.5380289560953742 1103 62-63 1.6911087086949692 1103 64-65 1.8019101208864043 1103 66-67 1.5808857753595866 1103 68-69 1.5628891498210855 1103 70-71 1.4638333311841407 1103 72-73 1.258684576778684 1103 74-75 1.4678034435497018 1103 76-77 1.535623116365155 1103 78-79 1.5925926623141962 1103 80-81 1.52813382641774 1103 82-83 1.4314673618417153 1103 84-85 1.6165797024052857 1103 86-87 1.707003488752747 1103 88-89 1.7224822946992475 1103 90-91 1.6613410766121213 1103 92-93 1.3655186514476654 1103 94-95 1.393825487704813 1103 96-97 1.6179552927312315 1103 98-99 1.7411146733594087 1103 100-101 1.8529948226532085 1103 102-103 1.7002481486211103 1103 104-105 1.8336302342722952 1103 106-107 1.9956212432955631 1103 108-109 2.266237681719648 1103 110-111 2.2186259211893606 1103 112-113 2.4948771684339484 1103 114-115 2.2977386952012004 1103 116-117 2.2168088567973605 1103 118-119 2.237838043132154 1103 120-121 2.2619065415017268 1104 1 1.0579793570574232 1104 2 1.2313573569754084 1104 3 1.2881312606665567 1104 4 1.490918261862653 1104 5 1.706561169074881 1104 6 1.52569407344361 1104 7 1.7282750008539978 1104 8 1.3548780572641164 1104 9 1.3628049808033964 1104 10-11 1.3496531914441903 1104 12-13 1.3849381799519378 1104 14-15 1.5387915880136873 1104 16-17 1.4934152027565446 1104 18-19 1.2903700326217091 1104 20-21 1.3704999599270167 1104 22-23 1.6357987970376442 1104 24-25 1.6591788152676301 1104 26-27 1.4118949630622453 1104 28-29 1.4101806484714245 1104 30-31 1.5314295588747768 1104 32-33 1.4843836118874947 1104 34-35 1.6622343918209097 1104 36-37 1.6925278045645413 1104 38-39 1.623081881729803 1104 40-41 1.7311361889934176 1104 42-43 1.7720474976546399 1104 44-45 1.6968065616758778 1104 46-47 1.7640978626226271 1104 48-49 1.6093609313333133 1104 50-51 1.806336880090754 1104 52-53 1.853879028674939 1104 54-55 1.9937570547799268 1104 56-57 1.9601201873774272 1104 58-59 2.073353380171625 1104 60-61 2.3019420538019872 1104 62-63 2.1722783193963906 1104 64-65 2.283521726567635 1104 66-67 2.3340015671308443 1104 68-69 2.3529485502046192 1104 70-71 2.152925331620679 1104 72-73 2.0375132511055547 1104 74-75 2.2856796849553582 1104 76-77 2.0883647324921277 1104 78-79 2.2741964726695088 1104 80-81 2.2544040747768257 1104 82-83 2.303423676835248 1104 84-85 2.142860084688067 1104 86-87 1.97895356748554 1104 88-89 2.1142636825790753 1104 90-91 1.8422799456662382 1104 92-93 2.193608328877083 1104 94-95 2.2306872453949005 1104 96-97 2.2506831890845795 1104 98-99 2.563206627023991 1104 100-101 2.740056920219505 1104 102-103 2.4038542663928943 1104 104-105 2.435684302652895 1104 106-107 2.375013519686636 1104 108-109 2.314762026522505 1104 110-111 2.4209130698150467 1104 112-113 2.62014026068384 1104 114-115 2.6723781613772815 1104 116-117 2.8148031194374816 1104 118-119 2.670002337421298 1104 120-121 2.5321050104997163 1105 1 0.9834230435472762 1105 2 1.347689548659293 1105 3 1.038254388974746 1105 4 1.315173123307929 1105 5 1.315641527986827 1105 6 1.193556169738443 1105 7 1.2969607830725707 1105 8 1.25295329290622 1105 9 1.3881156321096881 1105 10-11 1.3901072093897895 1105 12-13 1.4321401952934352 1105 14-15 1.6606263413324953 1105 16-17 1.5337729824842512 1105 18-19 1.4715229098613705 1105 20-21 1.6511942903637689 1105 22-23 1.3651967420686404 1105 24-25 1.7189469943839342 1105 26-27 1.5640079580521942 1105 28-29 1.6384364722988778 1105 30-31 1.6644477592201028 1105 32-33 2.051263512252632 1105 34-35 2.0727413407863438 1105 36-37 1.8054322173698907 1105 38-39 1.696606464933403 1105 40-41 2.0797322432264806 1105 42-43 2.0967439226879065 1105 44-45 1.9582178668359447 1105 46-47 1.8980784451469006 1105 48-49 2.2678723525453464 1105 50-51 2.119302149416235 1105 52-53 2.4028699426549593 1105 54-55 2.5818192699572577 1105 56-57 2.8732383056372086 1105 58-59 2.520323291575856 1105 60-61 2.362446172231607 1105 62-63 2.100303454554478 1105 64-65 2.295487816748505 1105 66-67 2.220369706619646 1105 68-69 2.3672710614560053 1105 70-71 2.3637139189002525 1105 72-73 2.5269312929408763 1105 74-75 2.613418935995579 1105 76-77 2.850684639650556 1105 78-79 2.7477974803402603 1105 80-81 2.9088098037813275 1105 82-83 2.70882575444854 1105 84-85 2.830732653965434 1105 86-87 2.7657024140421953 1105 88-89 2.6396465125487865 1105 90-91 2.5228539783306196 1105 92-93 2.3061136361317445 1105 94-95 2.615516988665302 1105 96-97 2.5904125898601436 1105 98-99 2.616064052934714 1105 100-101 2.910286759728127 1105 102-103 2.9692113384866445 1105 104-105 2.7725534469819166 1105 106-107 2.7072561532171626 1105 108-109 2.5138053054152003 1105 110-111 2.4896554627500294 1105 112-113 2.3154472322935646 1105 114-115 2.056245522468739 1105 116-117 1.955991661428481 1105 118-119 1.886324622229811 1105 120-121 1.7594819529549248 1106 1 0.6177461209058208 1106 2 0.7517655202407134 1106 3 0.8027085201417776 1106 4 1.0054955213378776 1106 5 1.0651617521652597 1106 6 1.3638864932686872 1106 7 1.1437268958977285 1106 8 0.9452570660104698 1106 9 0.8759245143310963 1106 10-11 1.0901779727852983 1106 12-13 0.9330431362201566 1106 14-15 1.1554096638154405 1106 16-17 0.9394793426982346 1106 18-19 1.0636936477820527 1106 20-21 1.1846399016179774 1106 22-23 1.2130582722563048 1106 24-25 1.1205490776583034 1106 26-27 1.0124780534412494 1106 28-29 1.1791310857892086 1106 30-31 0.9760359728689423 1106 32-33 1.1039171395842828 1106 34-35 1.4508641294302365 1106 36-37 1.5059388832817504 1106 38-39 1.408067304470336 1106 40-41 1.182302369751433 1106 42-43 1.4673827746225712 1106 44-45 1.4227540835417685 1106 46-47 1.7728442183077604 1106 48-49 2.0678157418289445 1106 50-51 1.9411765302365254 1106 52-53 1.92676532605104 1106 54-55 2.1154771713979983 1106 56-57 2.441898613033409 1106 58-59 2.3780181032036936 1106 60-61 2.5417379721693365 1106 62-63 2.354494062836622 1106 64-65 1.966466332981632 1106 66-67 2.0235059403086844 1106 68-69 1.863152631837277 1106 70-71 1.4903888884719976 1106 72-73 1.5790584406099306 1106 74-75 2.0349508219815995 1106 76-77 2.116061525495038 1106 78-79 2.1014559478881694 1106 80-81 2.0838501389167696 1106 82-83 2.5971554552609035 1106 84-85 2.2718688310437543 1106 86-87 2.0671459873106137 1106 88-89 2.10916164176275 1106 90-91 2.2562741147624443 1106 92-93 2.212558766194867 1106 94-95 1.9952645048701179 1106 96-97 1.8818785243615466 1106 98-99 2.2410491926216594 1106 100-101 2.4375787861087197 1106 102-103 2.414058348025552 1106 104-105 2.0967630198540625 1106 106-107 2.357520808316373 1106 108-109 1.8220506562601138 1106 110-111 1.8830121951794787 1106 112-113 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-0.7090259606737099 2316 74-75 -0.9684313119613961 2316 76-77 -0.6825625490062919 2316 78-79 -0.5408663458803176 2316 80-81 -0.3138101585012727 2316 82-83 -0.423803287948612 2316 84-85 -0.7872424804901073 2316 86-87 -0.9035474288019785 2316 88-89 -0.5094070601270531 2316 90-91 -1.0459628128965939 2316 92-93 -0.5536230405463307 2316 94-95 -0.5511520062358635 2316 96-97 -0.790264227908942 2316 98-99 -0.4414864115480661 2316 100-101 -1.0329206161187692 2316 102-103 -1.2891528439538114 2316 104-105 -0.9326222183110922 2316 106-107 -0.683059555381007 2316 108-109 -0.6490779801893289 2316 110-111 -0.47046537262050947 2316 112-113 -0.6867005640324848 2316 114-115 -1.0791320007554042 2316 116-117 -0.6764662557329366 2316 118-119 -0.4730802810320469 2316 120-121 -1.208493181500618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9777.0 3 1827.0 4 1485.0 5 1643.0 6 1909.0 7 1970.0 8 2315.0 9 2549.0 10 2940.0 11 3696.0 12 3713.0 13 4085.0 14 5027.0 15 5285.0 16 3865.0 17 3507.0 18 3766.0 19 3891.0 20 4122.0 21 4498.0 22 4933.0 23 5575.0 24 6537.0 25 7224.0 26 8381.0 27 9580.0 28 11300.0 29 13067.0 30 15557.0 31 18760.0 32 22870.0 33 29338.0 34 38418.0 35 52289.0 36 74616.0 37 90552.0 38 38059.0 39 248.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.403612550485356 19.078637455306467 13.734719251233656 21.783030742974525 2 13.951236052271584 21.382359704267234 39.7130753011696 24.953328942291577 3 20.853933546194092 26.10988207046975 30.611283955085213 22.424900428250943 4 11.170455982644057 16.209516582513693 41.88716691474769 30.73286052009456 5 10.891222034999785 37.96449432430645 36.11274336214311 15.031540278550665 6 26.33021775335813 37.30057434588385 19.672708453312996 16.696499447445017 7 24.376675785795175 32.89326728126739 24.543621993314368 18.186434939623066 8 22.760947871725563 31.68874436381398 21.87531014834578 23.674997616114677 9 22.444797347003117 19.11882047509507 25.694874220078855 32.741507957822954 10-11 23.25439957962546 24.861651040475646 29.36063172952012 22.523317650378775 12-13 26.2807469276184 23.308937226979154 28.610005809523532 21.800310035878915 14-15 22.153488349462602 25.3157979243603 29.854374364438733 22.67633936173836 16-17 19.489375367981587 28.899485677305087 30.07332869453514 21.53781026017819 18-19 18.67031169253318 30.32381882962748 30.50532398914278 20.50054548869656 20-21 20.166330890439625 27.609175937010328 28.98021353421511 23.244279638334937 22-23 19.104515669418625 27.433574920365817 30.094179040419032 23.367730369796526 24-25 21.362982081699506 26.995893905286337 29.610402755433835 22.030721257580314 26-27 20.678112598928735 25.28652953480744 32.61409350571988 21.421264360543944 28-29 19.081341911317352 29.07481010551154 30.973414284893813 20.870433698277296 30-31 19.694755768596135 27.43800639186638 30.178448526784802 22.68878931275268 32-33 20.143394646794547 27.04979873154634 30.888063588176024 21.918743033483086 34-35 21.782118456125595 25.979427209588952 30.365699554704033 21.87275477958142 36-37 21.16510128268031 25.753779117310344 32.07499045972446 21.006129140284884 38-39 20.74646583338769 27.4961618478427 31.161901386991854 20.595470931777758 40-41 19.849549544287186 28.55774341840102 30.746157935010544 20.84654910230125 42-43 19.16793542382979 28.84082970877643 28.83051091650345 23.160723950890336 44-45 19.895329242684877 27.27095727209627 28.82972602779733 24.003987457421527 46-47 21.441019380139302 28.306920031855633 29.162212525337495 21.089848062667578 48-49 21.145039059761825 27.391874946453466 29.718325765423348 21.744760228361358 50-51 18.82137704419763 28.152377538107537 31.56094039666713 21.465305021027703 52-53 19.83120021340935 29.187038886844363 28.969929921509753 22.011830978236528 54-55 19.027617940464577 27.670808481470882 29.905674889931323 23.395898688133222 56-57 20.987177491535977 26.489668054636727 29.110012841965986 23.413141611861306 58-59 22.390785140650156 26.374810901785672 28.95724799470768 22.27715596285649 60-61 21.1505546566036 26.801684707366796 31.109157432125883 20.938603203903725 62-63 20.02428815450928 27.623636834632144 30.529840150052877 21.822234860805697 64-65 19.149607526148692 29.871252994682614 29.652519428916463 21.326620050252235 66-67 19.788551266481694 27.620685591743836 28.28389758292828 24.306865558846187 68-69 19.180643301731727 28.127896922154104 29.226779830021265 23.464679946092904 70-71 21.461199680584386 27.142429528198956 28.41472938886901 22.98164140234765 72-73 20.616159262231385 25.821121532324664 31.031251614941414 22.531467590502537 74-75 19.184547054550418 28.846538249647757 29.692608464602436 22.27630623119939 76-77 19.75703362214721 27.132513109731722 29.2953019479823 23.81515132013877 78-79 20.321130428942812 27.108920971002487 29.893044120638525 22.676904479416176 80-81 21.414202363978767 26.743811465637634 29.039274748054755 22.802711422328844 82-83 20.354235665657466 26.296856421397873 30.918397058293657 22.430510854650997 84-85 20.271987365547165 27.39318016071538 29.860676336592885 22.474156137144572 86-87 20.586995360701785 28.029929071799593 29.104415852477132 22.278659715021494 88-89 19.50854008009885 27.41043894677701 28.33596726716463 24.745053705959506 90-91 19.65843759250892 26.344203293707057 29.135440848822896 24.861918264961126 92-93 21.339887547447614 27.824508580009 29.027326320791648 21.80827755175174 94-95 20.39852539372494 27.143992225794705 29.022693590759758 23.4347887897206 96-97 18.319173073271433 27.796890747710417 30.586259110849273 23.29767706816887 98-99 19.88218211423602 28.50573458935627 28.38468348007818 23.22739981632953 100-101 19.2586735121175 26.768640771593454 29.164775792657633 24.807909923631414 102-103 20.926068371907345 26.13847696756667 28.871211162283476 24.064243498242504 104-105 22.53468331063141 26.103055363803563 28.655731448116455 22.70652987744857 106-107 21.683197333024367 26.240676246566725 30.06968291692671 22.0064435034822 108-109 20.531562302832974 26.648900538241637 29.344198129006628 23.475339029918757 110-111 19.737387900563782 28.170186661992812 28.660064849706423 23.432360587736976 112-113 20.938112829820508 26.918358166228174 27.67464006745875 24.468888936492572 114-115 20.20229953105769 26.7987481888856 28.23556561297459 24.76338666708212 116-117 22.341312350271703 26.826636542469274 27.10320783748515 23.728843269773876 118-119 21.508401376939666 25.57923489285871 29.57508654562452 23.337277184577108 120-121 20.533430443431584 28.080754576621718 28.414732822271965 22.971082157674733 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1524.0 1 1671.5 2 1195.0 3 515.0 4 402.5 5 354.0 6 385.5 7 442.5 8 514.5 9 541.0 10 521.5 11 507.0 12 497.0 13 534.5 14 597.5 15 652.0 16 679.5 17 706.0 18 782.0 19 879.5 20 971.5 21 1103.5 22 1283.5 23 1518.5 24 1885.0 25 2420.5 26 3034.0 27 3832.0 28 4758.5 29 5860.5 30 7180.5 31 8433.0 32 9658.0 33 10882.0 34 11953.0 35 12950.5 36 14025.0 37 15088.5 38 16019.5 39 16590.5 40 17053.5 41 17574.0 42 17996.5 43 18416.0 44 18526.5 45 18758.5 46 20090.0 47 26283.0 48 40930.5 49 40117.5 50 25073.0 51 17728.0 52 13431.0 53 10852.0 54 9427.5 55 8247.5 56 7136.5 57 5990.0 58 4952.5 59 4192.5 60 3498.0 61 2786.0 62 2142.5 63 1598.5 64 1135.0 65 807.5 66 552.0 67 363.5 68 242.5 69 178.0 70 131.0 71 96.0 72 77.5 73 48.5 74 33.5 75 27.5 76 18.0 77 14.0 78 12.0 79 8.5 80 8.0 81 6.0 82 3.0 83 5.0 84 6.0 85 4.5 86 3.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23152160932558255 2 1.1572228193245426 3 1.0060211027516788 4 1.0071767846617896 5 1.0069841710101046 6 1.0012057614595493 7 1.008525080223586 8 1.0222006494932334 9 1.0285568999988444 10-11 1.029808888734798 12-13 1.0328907071617608 14-15 1.0317350252516497 16-17 1.0384765030606309 18-19 1.04290661704939 20-21 1.0476256515156768 22-23 1.0436770716561306 24-25 1.045025367217927 26-27 1.0561969590156672 28-29 1.0658276415999262 30-31 1.0694873009819443 32-33 1.0702577555886852 34-35 1.0753620173583422 36-37 1.0713171306729536 38-39 1.0748804832291294 40-41 1.0753620173583422 42-43 1.068620539549361 44-45 1.0716060511504815 46-47 1.0799847449987865 48-49 1.0790216767403606 50-51 1.1407543521054597 52-53 1.0799847449987865 54-55 1.0846074726392307 56-57 1.0081398529202157 58-59 1.0057321822741507 60-61 1.0811404269088976 62-63 1.1084915654481928 64-65 1.1102250883133593 66-67 1.1101287814875167 68-69 1.0975125873021376 70-71 1.2255043588469376 72-73 1.2297418591840117 74-75 1.2310901547458077 76-77 1.2302233933132245 78-79 1.0511890040718526 80-81 1.0442549126111862 82-83 1.103772530981906 84-85 1.089711734408888 86-87 1.0837407112066475 88-89 1.0947196893527027 90-91 1.0998239511223598 92-93 1.1004017920774152 94-95 1.0942381552234897 96-97 1.0991498033414617 98-99 1.1083952586223502 100-101 1.1061802016279705 102-103 1.0900006548864158 104-105 1.085281620420129 106-107 1.0850890067684436 108-109 1.0882671320212491 110-111 1.0938529279201192 112-113 1.0930824733133786 114-115 1.0936603142684342 116-117 1.1063728152796557 118-119 1.1010759398583134 120-121 1.0916378709257397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 519174.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.36060611086836 #Duplication Level Percentage of deduplicated Percentage of total 1 91.29410070452946 65.14802360621876 2 5.661680911216808 8.0804196286153 3 1.2062853442908246 2.582437599337523 4 0.564192648277052 1.610445173773856 5 0.32016387800963525 1.1423544194786843 6 0.20012637858657822 0.8568683804826798 7 0.1286541698184774 0.6426587675855994 8 0.10177854460958449 0.5810382905937602 9 0.07384903741401622 0.4742920863511553 >10 0.4027568530332812 5.102778659414262 >50 0.023472499600933335 1.1633717945107904 >100 0.014670310275970594 2.2940044861192 >500 0.003467527883411134 1.8314728813009173 >1k 0.004000993711628232 4.509207291417453 >5k 8.001987423256464E-4 3.9806269348000503 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8301 1.598885922638653 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7166 1.380269427975977 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5446 1.048973947077473 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4150 0.7993466544934837 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2540 0.48923867528034914 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1927 0.37116650679733576 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1816 0.3497863914602811 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1539 0.2964324099434872 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1365 0.2629176345502664 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1325 0.25521308848285934 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1314 0.2530943383143224 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1216 0.23421820044917502 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1202 0.23152160932558255 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1172 0.22574319977502724 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1096 0.21110456224695384 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1020 0.19646592471888039 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1006 0.1937693335952879 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1002 0.1929988789885472 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 954 0.1837534237076587 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 949 0.18279035544923283 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 902 0.17373751382002953 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 852 0.16410683123577066 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 845 0.1627585356739744 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 775 0.14927558005601205 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 736 0.14176364764029017 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 733 0.14118580668523462 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 630 0.1213466005616614 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 601 0.11576080466279128 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 556 0.10709319033695831 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 550 0.10593750842684727 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 539 0.10381875825831031 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.9261365168517683E-4 0.0 9 0.0 0.0 0.0 1.9261365168517683E-4 0.0 10-11 0.0 0.0 0.0 1.9261365168517683E-4 0.0 12-13 0.0 0.0 0.0 1.9261365168517683E-4 0.0 14-15 0.0 9.630682584258842E-5 0.0 1.9261365168517683E-4 0.0 16-17 0.0 1.9261365168517683E-4 0.0 4.8153412921294204E-4 0.0 18-19 0.0 1.9261365168517683E-4 0.0 7.704546067407073E-4 0.0 20-21 0.0 1.9261365168517683E-4 0.0 9.630682584258842E-4 0.0 22-23 0.0 1.9261365168517683E-4 0.0 0.0014446023876388261 0.0 24-25 0.0 1.9261365168517683E-4 0.0 0.002985511601120241 0.0 26-27 0.0 1.9261365168517683E-4 0.0 0.00481534129212942 0.0 28-29 0.0 3.8522730337035366E-4 0.0 0.008475000674147781 0.0 30-31 0.0 3.8522730337035366E-4 0.0 0.015601705786499324 0.0 32-33 0.0 3.8522730337035366E-4 0.0 0.0274474453651377 0.0 34-35 0.0 3.8522730337035366E-4 0.0 0.03736704842692431 0.0 36-37 0.0 3.8522730337035366E-4 0.0 0.044782674016803616 0.0 38-39 0.0 3.8522730337035366E-4 0.0 0.053546595168479164 0.0 40-41 0.0 3.8522730337035366E-4 0.0 0.07280796033699685 0.0 42-43 0.0 3.8522730337035366E-4 0.0 0.11065654289313409 0.0 44-45 0.0 3.8522730337035366E-4 0.0 0.16083239915712266 0.0 46-47 0.0 3.8522730337035366E-4 0.0 0.20811905064583358 0.0 48-49 0.0 3.8522730337035366E-4 0.0 0.31684945702211587 0.0 50-51 0.0 3.8522730337035366E-4 0.0 0.41305997603886174 0.0 52-53 0.0 3.8522730337035366E-4 0.0 0.5007954943814598 0.0 54-55 0.0 3.8522730337035366E-4 0.0 0.6844526112632758 0.0 56-57 0.0 3.8522730337035366E-4 0.0 0.8559750680889259 0.0 58-59 0.0 3.8522730337035366E-4 0.0 1.060434459352741 0.0 60-61 0.0 3.8522730337035366E-4 0.0 1.284636749914287 0.0 62-63 0.0 3.8522730337035366E-4 0.0 1.6526251314588172 0.0 64-65 0.0 3.8522730337035366E-4 0.0 1.8865544114304647 0.0 66-67 0.0 3.8522730337035366E-4 0.0 2.2927766028345022 0.0 68-69 0.0 3.8522730337035366E-4 0.0 2.8092701098283044 0.0 70-71 0.0 3.8522730337035366E-4 0.0 3.0191226833393046 0.0 72-73 0.0 5.778409550555305E-4 0.0 3.37651731404115 0.0 74-75 0.0 5.778409550555305E-4 0.0 3.892625593731581 0.0 76-77 0.0 5.778409550555305E-4 0.0 4.106619360753813 0.0 78-79 0.0 5.778409550555305E-4 0.0 4.293936137017647 0.0 80-81 0.0 7.704546067407073E-4 0.0 4.721442136932898 0.0 82-83 0.0 7.704546067407073E-4 0.0 5.4032944638984235 0.0 84-85 0.0 7.704546067407073E-4 0.0 6.302703910442356 0.0 86-87 0.0 7.704546067407073E-4 0.0 7.126801419177386 0.0 88-89 0.0 7.704546067407073E-4 0.0 7.860755738923752 0.0 90-91 0.0 7.704546067407073E-4 0.0 8.353461459934433 0.0 92-93 0.0 7.704546067407073E-4 0.0 8.836343884709173 0.0 94-95 0.0 7.704546067407073E-4 0.0 9.345614379764779 0.0 96-97 0.0 7.704546067407073E-4 0.0 10.116454213808858 0.0 98-99 0.0 7.704546067407073E-4 0.0 10.602322150184715 0.0 100-101 0.0 7.704546067407073E-4 0.0 10.820360803892337 0.0 102-103 0.0 7.704546067407073E-4 0.0 11.522726484762334 0.0 104-105 0.0 7.704546067407073E-4 0.0 12.384094735098444 0.0 106-107 0.0 7.704546067407073E-4 0.0 12.951630859788818 0.0 108-109 0.0 7.704546067407073E-4 0.0 13.693000805125063 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 665 0.0 71.83546 2 ACCTGGG 795 0.0 59.93664 3 GTACCTG 860 0.0 56.23839 1 TATAACG 60 2.6675745E-5 47.962425 2 TACACGG 85 8.044117E-8 47.398167 2 GTACACG 75 1.8422215E-6 46.06192 1 CCTGGGG 640 0.0 42.16398 4 ATAACGC 95 9.5395535E-6 36.25844 3 TCACGCA 100 1.3547959E-5 34.448868 4 ACACGGG 85 2.1089555E-4 33.770115 3 AGTACGC 105 1.860612E-5 32.888523 2 GTATAAC 180 3.018431E-8 28.788702 1 TATCACG 100 5.387877E-4 28.777458 2 GAGTACG 145 5.2436444E-6 27.795986 1 TAACCGT 105 7.278044E-4 27.335049 7 CTGGGGG 420 0.0 25.973354 5 TAACGCA 140 1.3439747E-4 24.606335 4 GTACGCA 140 1.3448636E-4 24.60394 3 TCTCGGG 250 0.0 24.14007 114-115 ACCGTGC 120 0.0015877117 23.91351 9 >>END_MODULE