##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003823_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 519174 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795105301883375 31.0 31.0 34.0 30.0 34.0 2 31.93458455161468 31.0 31.0 34.0 30.0 34.0 3 32.030766178583676 33.0 31.0 34.0 30.0 34.0 4 35.74460778082107 37.0 35.0 37.0 35.0 37.0 5 35.63138369795097 37.0 35.0 37.0 33.0 37.0 6 35.71203296004808 37.0 35.0 37.0 33.0 37.0 7 35.66512575745318 37.0 35.0 37.0 33.0 37.0 8 35.667138570113295 37.0 35.0 37.0 33.0 37.0 9 37.277689175498004 39.0 37.0 39.0 34.0 39.0 10-11 37.18422917942732 39.0 37.0 39.0 33.0 39.0 12-13 37.14618702015124 39.0 37.0 39.0 33.0 39.0 14-15 38.25799346654493 40.0 37.5 41.0 33.0 41.0 16-17 38.30535427428954 40.0 38.0 41.0 33.0 41.0 18-19 38.340176896377706 40.0 38.0 41.0 33.5 41.0 20-21 38.26943664359155 40.0 38.0 41.0 33.5 41.0 22-23 38.180026349547546 40.0 37.5 41.0 33.0 41.0 24-25 38.014539441497455 40.0 37.0 41.0 33.0 41.0 26-27 37.80564126863055 40.0 37.0 41.0 32.5 41.0 28-29 37.67165536024531 39.0 37.0 41.0 32.5 41.0 30-31 37.48869839398738 39.0 36.5 41.0 32.0 41.0 32-33 37.21466117332532 39.0 36.0 41.0 31.0 41.0 34-35 37.11303050615015 39.0 36.0 41.0 31.0 41.0 36-37 36.9181969821293 39.0 35.5 41.0 30.5 41.0 38-39 36.70116569782 39.0 35.0 40.0 30.0 41.0 40-41 36.80462426854966 39.0 36.0 41.0 30.5 41.0 42-43 36.66563907283492 39.0 35.0 41.0 30.0 41.0 44-45 36.30854010408842 39.0 35.0 41.0 30.0 41.0 46-47 36.16959150496751 39.0 35.0 41.0 30.0 41.0 48-49 35.92525242019053 39.0 35.0 41.0 28.5 41.0 50-51 35.644959108121746 39.0 35.0 40.0 28.0 41.0 52-53 35.48409396464384 39.0 35.0 40.0 27.0 41.0 54-55 35.31891716457295 39.0 35.0 40.0 27.0 41.0 56-57 35.01444602387639 38.0 34.0 40.0 26.5 41.0 58-59 34.72687769418346 38.0 34.0 40.0 26.0 41.0 60-61 34.307156175001055 38.0 34.0 40.0 24.5 41.0 62-63 34.1094527075701 37.5 33.5 40.0 24.5 41.0 64-65 33.6335120788021 37.0 33.0 39.5 22.5 41.0 66-67 33.2619208589028 36.5 32.5 39.0 22.0 41.0 68-69 32.92347459618548 36.0 32.0 39.0 22.0 40.5 70-71 32.4506909051686 35.0 32.0 38.5 20.5 40.0 72-73 32.033213335028336 35.0 31.0 37.5 20.0 40.0 74-75 31.666182243332678 35.0 31.0 37.0 18.5 39.5 76-77 30.67452145138239 34.0 29.5 36.0 18.0 39.0 78-79 31.08352594698502 34.5 31.0 36.0 18.0 39.0 80-81 30.81834702816397 34.0 31.0 36.0 15.5 38.5 82-83 30.506757849969375 34.0 30.5 35.5 12.5 37.0 84-85 30.19758597310343 34.0 30.0 35.0 8.5 37.0 86-87 29.926979201577893 34.0 30.0 35.0 7.0 36.5 88-89 29.609263946191447 34.0 30.0 35.0 2.0 36.0 90-91 29.293907244969894 34.0 30.0 35.0 2.0 36.0 92-93 29.016294151864308 34.0 29.0 35.0 2.0 35.5 94-95 28.63647640290153 34.0 29.0 35.0 2.0 35.0 96-97 28.24112243679383 34.0 29.0 35.0 2.0 35.0 98-99 27.924047621799243 34.0 29.0 35.0 2.0 35.0 100-101 27.05043106935247 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2048700209091585 1101 2 0.2889826126043076 1101 3 0.19580839475746004 1101 4 0.1663342310506195 1101 5 0.15765146065240287 1101 6 0.18561618944237068 1101 7 0.12616638203601838 1101 8 0.07009813434933676 1101 9 0.21522303847932278 1101 10-11 0.28270724677720693 1101 12-13 0.277653732491153 1101 14-15 0.4817742414967725 1101 16-17 0.3728997339751601 1101 18-19 0.24597057224843866 1101 20-21 0.22169392999693827 1101 22-23 0.18241181349990399 1101 24-25 0.2781335685990811 1101 26-27 0.4048172623393569 1101 28-29 0.29707706580335724 1101 30-31 0.223994406359175 1101 32-33 0.23481013857742994 1101 34-35 0.4194208335635139 1101 36-37 0.5059996912883946 1101 38-39 0.37281276159639987 1101 40-41 0.4790355060422016 1101 42-43 0.45760665207767204 1101 44-45 0.6952315084558194 1101 46-47 0.5256371371496869 1101 48-49 0.6720307057537624 1101 50-51 0.7719117414358649 1101 52-53 0.8099194555578677 1101 54-55 0.7103634610657323 1101 56-57 0.6046723665760325 1101 58-59 0.6114708809876817 1101 60-61 0.5826996967691827 1101 62-63 0.3727469340128877 1101 64-65 0.17041984215175177 1101 66-67 0.3257656631748844 1101 68-69 0.827356117503733 1101 70-71 0.7332316098295131 1101 72-73 0.460063464356228 1101 74-75 0.5787139226704632 1101 76-77 0.7044447886290577 1101 78-79 0.7701928541232732 1101 80-81 0.6893339848291475 1101 82-83 0.7758923088643108 1101 84-85 0.4479127212786871 1101 86-87 0.2757952798559167 1101 88-89 0.36538445687183696 1101 90-91 0.3961574121432001 1101 92-93 0.617232555854212 1101 94-95 0.5712166492352218 1101 96-97 0.535591255681819 1101 98-99 0.39580164797816053 1101 100-101 0.5666126398848697 1102 1 0.1234468965399742 1102 2 0.14973414659235473 1102 3 0.09307445156708383 1102 4 0.016201258718474776 1102 5 0.07293687054362152 1102 6 0.02404118824081536 1102 7 -0.06066455085300504 1102 8 0.11071877870441682 1102 9 4.567013063478953E-4 1102 10-11 0.0868838596675161 1102 12-13 0.14863812826807532 1102 14-15 0.1901126480716897 1102 16-17 0.1314015523317451 1102 18-19 0.06643629582619326 1102 20-21 0.06714683696436907 1102 22-23 -0.0052049378098004695 1102 24-25 0.057886528435027174 1102 26-27 -0.027953608709516686 1102 28-29 -0.009465012730416333 1102 30-31 0.031106023014359607 1102 32-33 2.289213198665152E-4 1102 34-35 0.15132978756790294 1102 36-37 0.22414440683974846 1102 38-39 0.13908264736836173 1102 40-41 0.15588510312002057 1102 42-43 0.3705209890583987 1102 44-45 0.44724291523901627 1102 46-47 0.45892788195494916 1102 48-49 0.4159812656794273 1102 50-51 0.6956833494398396 1102 52-53 0.5750242313472924 1102 54-55 0.7249751978766383 1102 56-57 0.4824868141000209 1102 58-59 0.4636837488644545 1102 60-61 0.4897408220675672 1102 62-63 0.38168772127318107 1102 64-65 0.28073440983147435 1102 66-67 0.30586707460409457 1102 68-69 0.5037455181669941 1102 70-71 0.5609999976601436 1102 72-73 0.5313154068737269 1102 74-75 0.47870310865549826 1102 76-77 0.4853644605238294 1102 78-79 0.5623218533062158 1102 80-81 0.5991479036679621 1102 82-83 0.2102776722953159 1102 84-85 0.38709990787251414 1102 86-87 0.5021890862289737 1102 88-89 0.5613744599157862 1102 90-91 0.7877032555562664 1102 92-93 0.4919742210523097 1102 94-95 0.594657428485128 1102 96-97 0.5757681249043323 1102 98-99 0.5445812663635898 1102 100-101 0.7674821667917442 1103 1 0.012440970828166087 1103 2 0.05767811041867077 1103 3 -0.06529174503440771 1103 4 0.01661936207775483 1103 5 0.10323026554290493 1103 6 0.08772194309803893 1103 7 0.05861362724870389 1103 8 0.01201128587928224 1103 9 0.12631097421944304 1103 10-11 0.11936378038272721 1103 12-13 0.09353675110787663 1103 14-15 0.1494002205945577 1103 16-17 0.14947239246141208 1103 18-19 -8.308567350781004E-4 1103 20-21 0.060507252383608545 1103 22-23 0.15315093525195778 1103 24-25 0.1521012167673561 1103 26-27 0.17534114899699915 1103 28-29 -0.06959034050280621 1103 30-31 0.09016699384003601 1103 32-33 0.2754230038668837 1103 34-35 0.324670794732441 1103 36-37 0.14197057907768595 1103 38-39 0.3028036640305558 1103 40-41 0.11065509465471735 1103 42-43 0.4258716797032065 1103 44-45 0.3464996203780757 1103 46-47 0.03396917611389938 1103 48-49 0.03620404766691365 1103 50-51 0.3565612632589321 1103 52-53 0.30050014652909596 1103 54-55 0.19216389153591962 1103 56-57 0.1054112664362421 1103 58-59 0.15936179895426505 1103 60-61 0.1796464649143914 1103 62-63 0.014875320637251832 1103 64-65 -0.2019834684746442 1103 66-67 -0.09128672549088179 1103 68-69 -0.19459342428959303 1103 70-71 -0.08415361661112186 1103 72-73 0.21173320052681888 1103 74-75 -0.01388820305345817 1103 76-77 0.10016893268124605 1103 78-79 0.24888679869468788 1103 80-81 0.29949219371813385 1103 82-83 0.4094889538595403 1103 84-85 0.1838836090679763 1103 86-87 0.27267769936464603 1103 88-89 0.22643134261972264 1103 90-91 0.3275417953948043 1103 92-93 0.20614018227709252 1103 94-95 0.459112448822502 1103 96-97 0.3702574193936279 1103 98-99 0.18041788602373643 1103 100-101 0.2577857820953575 1104 1 0.11239575793781498 1104 2 0.20262595968824826 1104 3 0.28882340752011615 1104 4 0.13146010356916094 1104 5 0.08262877819471726 1104 6 0.09517427468001927 1104 7 0.1676468509277882 1104 8 0.054891814714189024 1104 9 0.15485745774792292 1104 10-11 0.24882933283747377 1104 12-13 0.3211127165589929 1104 14-15 0.4356508247323134 1104 16-17 0.1802452210733847 1104 18-19 0.1248438287235274 1104 20-21 0.36037629090810697 1104 22-23 0.2259234208695844 1104 24-25 0.12614356097777346 1104 26-27 0.2589327674625679 1104 28-29 0.27238826912286385 1104 30-31 0.28945839871366985 1104 32-33 0.3557663100205204 1104 34-35 0.4017533160526554 1104 36-37 0.44318742945420553 1104 38-39 0.7589510580530998 1104 40-41 0.7699915565121884 1104 42-43 0.5529534214908267 1104 44-45 0.7487360993852619 1104 46-47 0.9471183318800911 1104 48-49 0.7388084660576482 1104 50-51 0.9468061923994142 1104 52-53 1.0326418343730595 1104 54-55 0.9254125156608879 1104 56-57 1.0266519705100805 1104 58-59 1.0177082544195741 1104 60-61 0.9377551629390481 1104 62-63 0.8212807399478308 1104 64-65 0.9123926715917676 1104 66-67 0.932846824032822 1104 68-69 0.9161243693213592 1104 70-71 0.9564475589240224 1104 72-73 0.9692931076065356 1104 74-75 1.2045422860252906 1104 76-77 0.968452869632646 1104 78-79 0.8701384162248189 1104 80-81 1.0501072449334252 1104 82-83 0.9840543644393556 1104 84-85 0.9018643078409916 1104 86-87 1.0253452598953494 1104 88-89 0.8492950335506428 1104 90-91 0.8063385088853963 1104 92-93 0.7024974264734745 1104 94-95 1.0100502265309856 1104 96-97 0.8211585590702768 1104 98-99 1.2273387322843199 1104 100-101 1.2965480402453728 1105 1 0.018421968699513513 1105 2 0.13899268390914443 1105 3 0.10563395975765744 1105 4 0.12779739021752334 1105 5 -0.3159899203249168 1105 6 0.034329076684038284 1105 7 0.11889087142086652 1105 8 -5.454663201476251E-5 1105 9 -0.0590065067005483 1105 10-11 -0.23470402502716325 1105 12-13 0.04001928057156334 1105 14-15 0.13469693416083572 1105 16-17 0.15179833607805193 1105 18-19 0.21631259376074752 1105 20-21 -0.13328330885412498 1105 22-23 0.0597030353506014 1105 24-25 0.10017764062904888 1105 26-27 0.26223175696127754 1105 28-29 0.07772807494810507 1105 30-31 0.027023854854519413 1105 32-33 0.28699561172732757 1105 34-35 0.17312103096109155 1105 36-37 0.1617826345265243 1105 38-39 0.22986871776608098 1105 40-41 0.24256932548385635 1105 42-43 0.4659215213521293 1105 44-45 0.468590892897673 1105 46-47 0.59881523810445 1105 48-49 0.7815339889991364 1105 50-51 0.7621788324401777 1105 52-53 0.8257636323294051 1105 54-55 0.8527837970444594 1105 56-57 0.8983919008788988 1105 58-59 0.9043283115963874 1105 60-61 1.1197741841397644 1105 62-63 1.0292670967651745 1105 64-65 1.1462487105399433 1105 66-67 1.0823543108000706 1105 68-69 1.0490335940376028 1105 70-71 1.2779200036578153 1105 72-73 1.2467209997065076 1105 74-75 1.0461554083893496 1105 76-77 0.9479286543987051 1105 78-79 1.1254923747291627 1105 80-81 1.0866268180886252 1105 82-83 1.1660748009079818 1105 84-85 1.0658315868469082 1105 86-87 1.0770803783532763 1105 88-89 1.2158296085456897 1105 90-91 1.1144791299049572 1105 92-93 1.0452710685239133 1105 94-95 1.0570201379352184 1105 96-97 1.180142679764323 1105 98-99 1.180412694185307 1105 100-101 1.2984742198711743 1106 1 -0.2811902478930861 1106 2 -0.102038763343824 1106 3 -0.1557830064740493 1106 4 -0.05804426960868625 1106 5 -0.09521378740295461 1106 6 0.10100517847011048 1106 7 0.23032906667122433 1106 8 -0.020909934556947007 1106 9 -0.09295595333079376 1106 10-11 -0.30437766424707036 1106 12-13 -0.30935375574421187 1106 14-15 -0.2961276009236613 1106 16-17 -0.003428248314371274 1106 18-19 0.009683478869298767 1106 20-21 0.04988066408595415 1106 22-23 -0.16328940711876072 1106 24-25 -0.11802553523214243 1106 26-27 -0.1412130051321796 1106 28-29 -0.04904030230570555 1106 30-31 -0.12089145551373548 1106 32-33 0.13127651410214725 1106 34-35 0.12842970089230477 1106 36-37 0.15091337697606377 1106 38-39 0.0016624283154911268 1106 40-41 0.36474374310110846 1106 42-43 0.6936239754266893 1106 44-45 0.5016515512803039 1106 46-47 0.5856022968946775 1106 48-49 0.5325402444833074 1106 50-51 0.4103630437113708 1106 52-53 0.644886732332246 1106 54-55 0.668852748897045 1106 56-57 0.2598881221135798 1106 58-59 0.36610475587729496 1106 60-61 0.060933005504651305 1106 62-63 0.05233030263004679 1106 64-65 0.09169296313695696 1106 66-67 0.08080600770629331 1106 68-69 0.4839086258811207 1106 70-71 0.4200044102359861 1106 72-73 0.18430768486600968 1106 74-75 0.10906123646260468 1106 76-77 -0.14379202832653348 1106 78-79 0.25206261447554823 1106 80-81 0.39267284260106194 1106 82-83 0.40825261516821953 1106 84-85 0.1278118297068822 1106 86-87 0.18205079925395395 1106 88-89 0.2662046545418981 1106 90-91 0.4346183922673177 1106 92-93 0.7075994672897998 1106 94-95 1.1908082440236996 1106 96-97 0.6688261975542424 1106 98-99 0.6755894611472932 1106 100-101 0.7856150956389563 1107 1 -0.29570149713671867 1107 2 -0.42832733807290424 1107 3 -0.05102476894723296 1107 4 -0.3052855920136608 1107 5 -0.1965174894767756 1107 6 -0.17537966118003823 1107 7 -0.3547856261061568 1107 8 -0.10967169262944765 1107 9 -0.2571013958743933 1107 10-11 -0.11411417340599428 1107 12-13 -0.17022069011903795 1107 14-15 -0.19591306767100747 1107 16-17 -0.48797085241800886 1107 18-19 -0.40195082444771657 1107 20-21 -0.42033221891091443 1107 22-23 -0.36975290896153723 1107 24-25 -0.3442260963191117 1107 26-27 -0.602542011128115 1107 28-29 -0.5719513535186493 1107 30-31 -0.26057460640212327 1107 32-33 -0.3384302904525569 1107 34-35 -0.7357267433565511 1107 36-37 -0.7726192705843076 1107 38-39 -0.6326518904039133 1107 40-41 -0.12962338380356186 1107 42-43 -0.4719791930644277 1107 44-45 -0.1829020545285971 1107 46-47 -0.478653162701562 1107 48-49 -0.2057896966575683 1107 50-51 -0.3156009807124107 1107 52-53 -0.4443591007718908 1107 54-55 -0.22179030077565898 1107 56-57 -0.21706880621031388 1107 58-59 -0.3428781364710147 1107 60-61 -0.7722756418356838 1107 62-63 -0.012205700450429902 1107 64-65 0.29354675047400036 1107 66-67 0.343482722597102 1107 68-69 -0.3562420974809939 1107 70-71 -0.2910853086704499 1107 72-73 0.2171917819010467 1107 74-75 0.22171869836920166 1107 76-77 -0.23186617982001678 1107 78-79 -0.007737154488644649 1107 80-81 -0.061147499551967854 1107 82-83 -0.2707451294822043 1107 84-85 0.09193526883994707 1107 86-87 0.03610217721753628 1107 88-89 0.010095957693884827 1107 90-91 -0.37485957681789017 1107 92-93 -0.007632393648645319 1107 94-95 0.18905897288667006 1107 96-97 0.002161364505116836 1107 98-99 1.2326388672079247E-4 1107 100-101 -0.22986430366793442 1108 1 -0.2506554666928231 1108 2 0.12170456421494436 1108 3 0.036059361943298285 1108 4 -0.2933085616609077 1108 5 -0.49463069702394336 1108 6 -0.4787613036921172 1108 7 -0.507533780330931 1108 8 -0.027783077188743732 1108 9 -0.3327553927753115 1108 10-11 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86-87 -1.009425174486008 2316 88-89 -0.4690135848607504 2316 90-91 -1.0983901862925762 2316 92-93 -0.7352555781199364 2316 94-95 -1.2314446242356283 2316 96-97 -0.8532546800177521 2316 98-99 -0.26835101329530886 2316 100-101 -0.3381919811485936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 13.0 6 23.0 7 73.0 8 213.0 9 353.0 10 622.0 11 1044.0 12 1673.0 13 2392.0 14 2448.0 15 3142.0 16 3784.0 17 5001.0 18 4428.0 19 3264.0 20 2996.0 21 3048.0 22 3254.0 23 3523.0 24 4029.0 25 4434.0 26 5504.0 27 6769.0 28 8527.0 29 10673.0 30 13170.0 31 16263.0 32 20273.0 33 25750.0 34 33655.0 35 46383.0 36 68376.0 37 98413.0 38 94680.0 39 20961.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.86032705488694 19.597657725727238 13.695327016337325 19.846688203048505 2 15.313406501966615 24.48696563334271 38.22226416984163 21.977363694849046 3 22.91068197975851 26.905033013315144 29.46941716615321 20.71486784077313 4 12.22018051751436 17.89245994599113 40.88648507051586 29.000874465978647 5 12.018128796896608 39.91108953838212 33.89615042355742 14.174631241163848 6 26.96109589463263 39.83654805517996 17.794804824586745 15.407551225600665 7 27.78606016480024 32.835619657378835 22.340872231660292 17.037447946160633 8 24.2833809089053 32.34214348176142 20.239072064471642 23.135403544861646 9 23.363843335760265 21.60855512795325 23.201855254693033 31.825746281593453 10-11 25.231425302499737 25.634854595954344 28.788806835473267 20.344913266072645 12-13 28.574620454799355 23.780948198484516 27.630717254716146 20.013714091999983 14-15 21.859846503963563 27.197922053907565 29.388933993237238 21.553297448891637 16-17 19.57561434124205 29.310211990585046 29.412200919152347 21.70197274902056 18-19 19.459275695624203 31.711718999795828 29.561476499208357 19.267528805371608 20-21 22.105899883163953 27.260459619324468 28.466288514718162 22.167351982793413 22-23 20.205846209555947 28.726785239630644 28.837056555220407 22.230311995593 24-25 23.221790767642446 28.259600827468244 28.985465373843837 19.53314303104547 26-27 22.426670647551997 26.45924327864432 32.10966054926586 19.004425524537822 28-29 20.096761578930135 30.81961276104725 29.829940032093326 19.253685627929286 30-31 21.694251491930668 28.108975470309712 28.85725238188802 21.3395206558716 32-33 21.82829262467098 28.742489018244015 29.644047604142486 19.785170752942513 34-35 23.03457574882019 26.498025618799964 30.641722045651548 19.825676586728306 36-37 22.62470114302558 26.62692824157469 31.72738849576447 19.020982119635267 38-39 21.027197317841555 28.829253253947606 31.10407815254776 19.03947127566307 40-41 20.170950048877266 29.348126531929136 29.587167670697234 20.89375574849636 42-43 20.14556865101953 29.49561435190937 27.952860182244937 22.40595681482616 44-45 22.281946548600633 27.494271826052284 28.072434553385428 22.15134707196166 46-47 23.359366196572676 29.17895835801303 28.084094912564062 19.37758053285024 48-49 22.74057195248561 29.037172866638798 28.5944461244797 19.627809056395897 50-51 20.120173046104536 29.437388282790927 30.905410194773175 19.53702847633136 52-53 21.690424069888273 30.604434180227535 27.393260223259556 20.31188152662463 54-55 20.010863587815447 29.356053127567222 28.33200588251723 22.3010774021001 56-57 22.95274293629129 27.33737625145204 28.742412304444066 20.967468507812605 58-59 24.642249993980112 27.2128873799032 27.93681523754485 20.208047388571842 60-61 21.223418599275753 28.853436320209568 30.642961707373452 19.280183373141227 62-63 20.42220287465473 28.438067023911795 29.763482408053036 21.376247693380435 64-65 20.1380574786436 31.4353080893084 28.459196795046047 19.96743763700195 66-67 21.83274125932071 27.437644345139162 27.401512896036813 23.328101499503312 68-69 20.585781475494173 29.489844874460257 27.829822408135662 22.094551241909908 70-71 23.30885660645257 28.787173848336767 27.44986413056566 20.454105414645003 72-73 22.06437533466622 27.215731142160433 30.177743877775082 20.54214964539827 74-75 20.347128323067025 31.049224344824665 28.154241159996456 20.449406172111857 76-77 21.74213269539692 27.987437737637094 27.524587132637613 22.745842434328374 78-79 21.72133042101492 29.085624472720127 28.159923262721172 21.033121843543785 80-81 22.584721114693725 27.62310901547458 28.596771024743152 21.195398845088544 82-83 21.690704850396976 27.306259558452467 30.15058535288747 20.85245023826309 84-85 20.36484877902206 28.849094908450727 29.173359991062725 21.61269632146448 86-87 20.35085353815039 29.060650567018996 27.51768473262225 23.070811162208365 88-89 20.314769229583916 29.008867932523586 26.870471171514755 23.805891666377747 90-91 21.747819102523852 26.835103155632 27.595359489335802 23.821718252508344 92-93 22.39710539089165 29.25552610695492 27.37214256546963 20.9752259366838 94-95 21.746642461248054 29.372601328087754 27.40156115319237 21.479195057471816 96-97 19.551172775655733 29.743447500062604 29.226836789925127 21.478542934356536 98-99 21.319477217347753 30.465091641063673 26.02519527299695 22.190235868591625 100-101 19.3709824378235 28.998973276288847 26.80259358499943 24.82745070088822 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 119.0 1 89.5 2 154.0 3 223.5 4 156.0 5 115.5 6 152.5 7 245.5 8 375.0 9 453.5 10 461.0 11 456.0 12 479.5 13 502.5 14 571.0 15 665.5 16 699.0 17 740.0 18 813.5 19 915.0 20 1019.5 21 1129.0 22 1291.5 23 1567.5 24 1924.0 25 2354.5 26 2977.5 27 3728.5 28 4825.0 29 6108.0 30 7221.0 31 8413.5 32 9600.0 33 10816.5 34 11971.0 35 13144.0 36 14260.5 37 15248.0 38 16254.0 39 16717.0 40 17011.5 41 17492.0 42 17966.5 43 18571.0 44 18662.5 45 18590.0 46 19635.5 47 26585.5 48 44497.0 49 43162.0 50 25085.5 51 17905.0 52 13897.0 53 11007.5 54 9163.0 55 7929.5 56 6832.0 57 5762.0 58 4834.5 59 4058.0 60 3279.5 61 2543.0 62 1950.5 63 1402.5 64 905.5 65 596.5 66 387.0 67 227.0 68 148.5 69 89.5 70 45.0 71 19.5 72 12.5 73 7.0 74 4.0 75 4.0 76 2.5 77 1.0 78 1.0 79 1.0 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06972614191003401 2 0.0015409092134814146 3 0.027158524887609933 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014446023876388263 16-17 0.0 18-19 0.0 20-21 0.013579262443804967 22-23 0.0 24-25 0.0 26-27 0.005104261769657186 28-29 0.012134660056166141 30-31 0.00924545528088849 32-33 0.0033707389044905943 34-35 0.004622727640444245 36-37 0.021765342640424984 38-39 0.03640398016849842 40-41 0.003178125252805418 42-43 0.0072230119381941306 44-45 0.0056821027247127166 46-47 0.004333807162916479 48-49 9.630682584258843E-4 50-51 0.0011556819101110611 52-53 4.8153412921294215E-4 54-55 0.0016372160393240032 56-57 0.014831251179758616 58-59 0.011845739578638374 60-61 0.0026965911235924756 62-63 0.0030818184269628293 64-65 0.09187671185382935 66-67 0.045360514971859146 68-69 0.033707389044905944 70-71 0.021091194859526863 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.002215056994379534 88-89 0.0 90-91 0.0013482955617962378 92-93 3.8522730337035366E-4 94-95 0.0012519887359536495 96-97 0.004044886685388714 98-99 0.005585795898870129 100-101 0.009052841629203312 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 519174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.23769841570036 #Duplication Level Percentage of deduplicated Percentage of total 1 88.42810965279033 58.57274458652007 2 7.652506193654034 10.137687947590699 3 1.5814955444714558 3.1426387476142237 4 0.6867567497360116 1.8195674589584216 5 0.3916879107941518 1.2972252854129385 6 0.26003970443041313 1.033465891090174 7 0.18128065395773746 0.8405329299817487 8 0.12631797260101663 0.6693609418903071 9 0.10021448567787462 0.5974179191294023 >10 0.534127865292217 6.256998032626799 >50 0.030197533520414304 1.3620882250201491 >100 0.017883975457405128 2.3870527440525375 >500 0.0032249791808435396 1.4655944565054084 >1k 0.0058635985106246175 7.093772346851207 >5k 0.0 0.0 >10k+ 2.931799255312309E-4 3.3238524867558725 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17116 3.296775262243487 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3664 0.705736419774488 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3466 0.667598916740823 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3142 0.6051920935948256 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2283 0.4397369667972587 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2123 0.4089187825276304 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2040 0.39293184943776077 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1940 0.37367048426924304 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1938 0.37328525696587267 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1881 0.36230627881981764 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1644 0.3166568433704307 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1604 0.30895229730302365 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1562 0.3008625239322462 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1527 0.294121046123265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1376 0.26503638471880336 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1145 0.2205426311795275 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1098 0.21148978955032416 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1065 0.20513353904471335 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1015 0.1955028564604545 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1013 0.19511762915708414 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1003 0.19319149264023236 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 976 0.1879909240447326 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 847 0.1631437629773448 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 822 0.15832842168521535 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 710 0.13675569269647556 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 709 0.13656307904479037 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 647 0.12462103264030942 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 647 0.12462103264030942 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 585 0.11267898623582846 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.10921194050549526 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 525 0.10112216713471785 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.9261365168517683E-4 0.0 9 0.0 0.0 0.0 1.9261365168517683E-4 0.0 10-11 0.0 0.0 0.0 1.9261365168517683E-4 0.0 12-13 0.0 0.0 0.0 1.9261365168517683E-4 0.0 14-15 0.0 0.0 0.0 1.9261365168517683E-4 0.0 16-17 0.0 0.0 0.0 6.741477808981189E-4 0.0 18-19 0.0 0.0 0.0 9.630682584258841E-4 0.0 20-21 0.0 0.0 0.0 0.001155681910111061 0.0 22-23 0.0 0.0 0.0 0.0016372160393240032 0.0 24-25 0.0 0.0 0.0 0.0032744320786480064 0.0 26-27 0.0 0.0 0.0 0.005007954943814598 0.0 28-29 0.0 0.0 0.0 0.008571307499990369 0.0 30-31 0.0 0.0 0.0 0.016950001348295562 0.0 32-33 0.0 0.0 0.0 0.030240343314572763 0.0 34-35 0.0 0.0 0.0 0.04054517367972972 0.0 36-37 0.0 0.0 0.0 0.048731253876349745 0.0 38-39 0.0 0.0 0.0 0.05759148185386788 0.0 40-41 0.0 0.0 0.0 0.07656392654485779 0.0 42-43 0.0 0.0 0.0 0.1147014295785228 0.0 44-45 0.0 0.0 0.0 0.1670923428368909 0.0 46-47 0.0 0.0 0.0 0.21486052845481476 0.0 48-49 0.0 0.0 0.0 0.32705798056143026 0.0 50-51 0.0 0.0 0.0 0.4277949203927778 0.0 52-53 0.0 0.0 0.0 0.518997484465709 0.0 54-55 0.0 0.0 0.0 0.709684999634034 0.0 56-57 0.0 0.0 0.0 0.889875070785517 0.0 58-59 0.0 0.0 0.0 1.103772530981906 0.0 60-61 0.0 0.0 0.0 1.3350052198299607 0.0 62-63 0.0 0.0 0.0 1.7162839433407682 0.0 64-65 0.0 0.0 0.0 1.9601328263742022 0.0 66-67 0.0 0.0 0.0 2.3793564392669895 0.0 68-69 0.0 0.0 0.0 2.918289436682114 0.0 70-71 0.0 0.0 0.0 3.140180363423438 0.0 72-73 0.0 0.0 0.0 3.5150065296027924 0.0 74-75 0.0 0.0 0.0 4.05442106114713 0.0 76-77 0.0 0.0 0.0 4.285750056821028 0.0 78-79 0.0 0.0 0.0 4.486357175051139 0.0 80-81 0.0 0.0 0.0 4.933798687915805 0.0 82-83 0.0 0.0 0.0 5.650417779010505 0.0 84-85 0.0 0.0 0.0 6.589024103672372 0.0 86-87 0.0 0.0 0.0 7.443169342070289 0.0 88-89 0.0 0.0 0.0 8.206882471002015 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 130 0.0 65.767296 2 TACCTGG 840 0.0 59.373257 2 GTATAAC 170 0.0 55.91842 1 ATAACGC 170 0.0 55.880707 3 ACCTGGG 915 0.0 52.430153 3 GTACCTG 1005 0.0 49.658897 1 TAACGCA 215 0.0 46.38058 4 CCTGGGG 565 0.0 42.022015 4 TACCCGG 55 0.0025786997 34.544437 2 GTACCCG 60 0.0039365184 31.687103 1 GGAGCGT 60 0.0039551337 31.656584 8 CGTGACG 40 4.44364E-4 29.743937 64-65 GTACACG 85 6.378294E-4 27.95921 1 TACACGG 85 6.403509E-4 27.940353 2 AGTACGC 145 7.8756057E-7 26.206125 2 CCGATCT 55 9.4962976E-5 25.900843 88-89 CTGGGGG 315 0.0 24.119305 5 GCGTGAC 50 0.0016353581 23.795149 62-63 ACTGTCC 160 1.8614337E-6 23.74244 8 CCTGGGA 485 0.0 23.497671 4 >>END_MODULE