##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003822_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 818431 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.764289231468506 31.0 31.0 34.0 30.0 34.0 2 31.92494541384674 31.0 31.0 34.0 30.0 34.0 3 32.01390465414922 33.0 31.0 34.0 30.0 34.0 4 35.72125933646208 37.0 35.0 37.0 35.0 37.0 5 35.60918269224895 37.0 35.0 37.0 33.0 37.0 6 35.69022678759725 37.0 35.0 37.0 33.0 37.0 7 35.64554617310439 37.0 35.0 37.0 33.0 37.0 8 35.649217832657854 37.0 35.0 37.0 33.0 37.0 9 37.25093502078978 39.0 37.0 39.0 33.0 39.0 10-11 37.156782917558104 39.0 37.0 39.0 33.0 39.0 12-13 37.12855390374998 39.0 37.0 39.0 33.0 39.0 14-15 38.1997107880811 40.0 37.5 41.0 33.0 41.0 16-17 38.261164349835234 40.0 38.0 41.0 33.0 41.0 18-19 38.3035161180356 40.0 38.0 41.0 33.5 41.0 20-21 38.237505055404796 40.0 37.5 41.0 33.5 41.0 22-23 38.1606800084552 40.0 37.5 41.0 33.0 41.0 24-25 37.989528744634555 40.0 37.0 41.0 33.0 41.0 26-27 37.76015876720212 39.5 37.0 41.0 32.5 41.0 28-29 37.641029604206096 39.0 37.0 41.0 32.0 41.0 30-31 37.47761692800004 39.0 36.5 41.0 32.0 41.0 32-33 37.224081199270316 39.0 36.0 41.0 31.0 41.0 34-35 37.136731746475874 39.0 36.0 41.0 31.0 41.0 36-37 36.95064825257108 39.0 35.5 40.5 30.5 41.0 38-39 36.749821304422724 39.0 35.0 40.0 30.0 41.0 40-41 36.8757494523057 39.0 36.0 41.0 30.5 41.0 42-43 36.742775505815395 39.0 35.0 41.0 30.0 41.0 44-45 36.37023829742519 39.0 35.0 41.0 30.0 41.0 46-47 36.25016769892636 39.0 35.0 41.0 30.0 41.0 48-49 35.98337856215124 39.0 35.0 41.0 29.0 41.0 50-51 35.68436496173777 39.0 35.0 40.0 28.0 41.0 52-53 35.53567680109869 39.0 35.0 40.0 28.0 41.0 54-55 35.38522367798873 38.5 34.5 40.0 27.5 41.0 56-57 35.05507550422699 38.0 34.0 40.0 26.5 41.0 58-59 34.769613443283546 38.0 34.0 40.0 26.0 41.0 60-61 34.30155016122312 38.0 33.5 40.0 25.0 41.0 62-63 34.13177286784103 37.0 33.0 40.0 25.0 41.0 64-65 33.65867739613969 37.0 33.0 39.5 23.0 41.0 66-67 33.25503493880363 36.0 32.0 39.0 22.0 41.0 68-69 32.90462910129259 36.0 32.0 39.0 22.0 40.0 70-71 32.40029580990945 35.0 31.5 38.5 21.5 40.0 72-73 31.967672290028116 35.0 31.0 37.0 20.5 40.0 74-75 31.596926924811008 35.0 31.0 37.0 19.5 39.0 76-77 30.61359479296361 34.0 29.5 36.0 19.0 39.0 78-79 31.03563037079485 34.0 31.0 36.0 18.5 39.0 80-81 30.776681846117754 34.0 31.0 36.0 18.0 37.5 82-83 30.478793569647287 34.0 30.5 35.0 15.5 37.0 84-85 30.198320933591226 34.0 30.0 35.0 12.0 37.0 86-87 29.96236029671408 34.0 30.0 35.0 7.5 36.0 88-89 29.64218730717678 34.0 30.0 35.0 6.5 36.0 90-91 29.305102079466685 34.0 29.0 35.0 2.0 35.5 92-93 29.062566667196137 34.0 29.0 35.0 2.0 35.0 94-95 28.711495532305108 34.0 29.0 35.0 2.0 35.0 96-97 28.312161929350182 34.0 29.0 35.0 2.0 35.0 98-99 28.01992898607213 34.0 29.0 35.0 2.0 35.0 100-101 27.10746354915686 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.08719179047932002 1101 2 0.1271345956790455 1101 3 0.12760435457116515 1101 4 0.08806414509807325 1101 5 0.17032213354546855 1101 6 0.1048375289513288 1101 7 0.1104557018317891 1101 8 2.7863504777059234E-4 1101 9 0.15957678992522517 1101 10-11 0.2158556139478378 1101 12-13 0.15831912455642794 1101 14-15 0.2923046686968078 1101 16-17 0.24944944215621945 1101 18-19 0.1900797264574976 1101 20-21 0.18146567233521438 1101 22-23 0.07928206997910792 1101 24-25 0.14401067374461718 1101 26-27 0.07583095161632514 1101 28-29 0.11666006989929656 1101 30-31 0.2957255195070232 1101 32-33 0.23802077587288295 1101 34-35 0.1340438763623979 1101 36-37 0.15468501874081397 1101 38-39 0.21239201936847252 1101 40-41 0.23318871433204436 1101 42-43 0.3872154050683321 1101 44-45 0.41564742367481955 1101 46-47 0.37690916712173106 1101 48-49 0.4844738879012169 1101 50-51 0.32425804048595097 1101 52-53 0.36870666798279217 1101 54-55 0.48689620988651683 1101 56-57 0.4498254331203171 1101 58-59 0.4791221984545402 1101 60-61 0.29819979544024733 1101 62-63 0.29968486583373277 1101 64-65 0.5036112636519547 1101 66-67 0.35478587025495045 1101 68-69 0.5394340833752693 1101 70-71 0.5396073517532045 1101 72-73 0.6049286336768489 1101 74-75 0.46114083671372086 1101 76-77 0.3723409350566804 1101 78-79 0.5593716644941971 1101 80-81 0.5575717700183205 1101 82-83 0.5353069393463272 1101 84-85 0.4672688569921526 1101 86-87 0.314104581656224 1101 88-89 0.4009745527698172 1101 90-91 0.446866612936077 1101 92-93 0.5825109205058254 1101 94-95 0.40438870779401626 1101 96-97 0.4478910249518222 1101 98-99 0.4817018097934209 1101 100-101 0.3176735763435161 1102 1 -0.0518171976410855 1102 2 0.16261516688160071 1102 3 0.08816846482907792 1102 4 -0.06393420003301742 1102 5 -0.06551532559426931 1102 6 -0.04409521699447083 1102 7 0.14353807702330101 1102 8 0.03982409165973877 1102 9 0.07207377797288927 1102 10-11 0.13578269060373316 1102 12-13 0.042382145965156326 1102 14-15 0.08891991622245854 1102 16-17 0.07396650685522843 1102 18-19 0.07980317240375001 1102 20-21 -0.0664782439757019 1102 22-23 0.054161705544238714 1102 24-25 -0.1140054384955036 1102 26-27 -0.06214738051222923 1102 28-29 0.037852848487844426 1102 30-31 0.12877697956512435 1102 32-33 0.040254394261928894 1102 34-35 0.22310537474616865 1102 36-37 0.0362673280103607 1102 38-39 0.10089454486209348 1102 40-41 0.16442641079089526 1102 42-43 0.4099466661784561 1102 44-45 0.4073864908561333 1102 46-47 0.3880997634655614 1102 48-49 0.34405803389338985 1102 50-51 0.41753195311432734 1102 52-53 0.40363144873539625 1102 54-55 0.5548774516746775 1102 56-57 0.693076386715795 1102 58-59 0.3748943266432363 1102 60-61 0.32000473913378613 1102 62-63 0.3155247799987748 1102 64-65 0.21515351963835627 1102 66-67 0.294058318960829 1102 68-69 0.1947057955735687 1102 70-71 0.3064547355600382 1102 72-73 0.44919887740630315 1102 74-75 0.4265224794893534 1102 76-77 0.40319514747092455 1102 78-79 0.5415459076417193 1102 80-81 0.521393562459572 1102 82-83 0.3154426022258896 1102 84-85 0.3194199592530502 1102 86-87 0.3278049867353978 1102 88-89 0.31240428676327525 1102 90-91 0.3199949971202578 1102 92-93 0.2702826213382643 1102 94-95 0.26403672990742777 1102 96-97 0.5358786770838293 1102 98-99 0.3464076386599757 1102 100-101 0.5209776312269696 1103 1 -0.11220012163114035 1103 2 -0.01874558215192934 1103 3 -0.03654410864432833 1103 4 -0.03279572751338833 1103 5 0.008819761040875562 1103 6 -0.1307543147626049 1103 7 0.06894969576159582 1103 8 0.10527051295996159 1103 9 0.13819325638954894 1103 10-11 0.08193093562526599 1103 12-13 0.08747367933776928 1103 14-15 -0.04412503249586308 1103 16-17 0.05576222803233577 1103 18-19 -0.24307442221945763 1103 20-21 -0.15505860843254027 1103 22-23 0.09547387949476871 1103 24-25 0.03405844444525741 1103 26-27 -0.08410257550717404 1103 28-29 -0.12006886610719647 1103 30-31 0.026480980542366694 1103 32-33 0.09508699226239514 1103 34-35 0.010275013882164785 1103 36-37 0.06689112740580327 1103 38-39 0.02633849908462338 1103 40-41 -0.09899131815630824 1103 42-43 0.15023105077430898 1103 44-45 0.09526792980913257 1103 46-47 0.3637235987893277 1103 48-49 0.1529409506977828 1103 50-51 0.14761598944504328 1103 52-53 0.010869387599775848 1103 54-55 0.10207168973091285 1103 56-57 -0.030120490874033123 1103 58-59 0.24803383868879791 1103 60-61 0.061421165080737694 1103 62-63 0.193299942993562 1103 64-65 0.14369269826914888 1103 66-67 0.14050488876475953 1103 68-69 0.06084647169656421 1103 70-71 -0.12477996496547306 1103 72-73 -0.07217936871703046 1103 74-75 -0.14526439134595392 1103 76-77 -0.1683111830983215 1103 78-79 -0.0052486279009649195 1103 80-81 -0.0463070612559946 1103 82-83 0.14898731381732588 1103 84-85 -0.08046069885788754 1103 86-87 -0.08617274489445137 1103 88-89 0.03313588308230209 1103 90-91 0.03706400104363183 1103 92-93 0.007172678296615942 1103 94-95 0.02306042582932477 1103 96-97 -0.1659704934511197 1103 98-99 -0.07499677525338555 1103 100-101 0.2988714774250347 1104 1 5.87014103093253E-4 1104 2 0.022577472051111158 1104 3 0.031635549497824655 1104 4 0.06769446856504402 1104 5 -0.040589152412650265 1104 6 0.04741971398387079 1104 7 -0.044994390448579225 1104 8 -0.01256845707995069 1104 9 0.007742977844124255 1104 10-11 0.028857431801668554 1104 12-13 0.04605832497828288 1104 14-15 -0.05637079582729143 1104 16-17 -0.0019701490348182915 1104 18-19 -0.10218051805077977 1104 20-21 3.7759662367875535E-4 1104 22-23 -0.03355808377525449 1104 24-25 -0.013463342047117521 1104 26-27 -0.17200705124228932 1104 28-29 0.02361232001378255 1104 30-31 0.0590263812529912 1104 32-33 -0.005379443379723625 1104 34-35 -0.11470305340739628 1104 36-37 0.08474343984457278 1104 38-39 0.1730500546986704 1104 40-41 0.27439571376309857 1104 42-43 0.18012716283583785 1104 44-45 0.23858082916722623 1104 46-47 0.16389832938252624 1104 48-49 0.22244205464421185 1104 50-51 0.413967738944244 1104 52-53 0.16060126021459098 1104 54-55 0.010952244189830651 1104 56-57 0.007112112831130446 1104 58-59 0.02079268122655975 1104 60-61 0.18850203098414653 1104 62-63 -0.053056485483864435 1104 64-65 0.05027945676076939 1104 66-67 0.11158478231800473 1104 68-69 0.08341157529206811 1104 70-71 0.01869925744683343 1104 72-73 0.17502146911250804 1104 74-75 0.1246477681487157 1104 76-77 0.0725081498548441 1104 78-79 0.1311061190686651 1104 80-81 0.2339646484718969 1104 82-83 0.2470064245190251 1104 84-85 0.2690416113876104 1104 86-87 0.353198846396797 1104 88-89 0.1881489695995313 1104 90-91 0.2040623999557667 1104 92-93 0.3681713842666987 1104 94-95 0.18329258356937927 1104 96-97 0.01874222459657915 1104 98-99 0.03040086478696935 1104 100-101 0.46348834952808815 1105 1 -0.10433623590700591 1105 2 -0.13911652873829894 1105 3 0.12311395414229409 1105 4 -0.16421512836930674 1105 5 -0.18068779767198606 1105 6 0.08175130999206459 1105 7 -0.01958872969005654 1105 8 0.09503226271343124 1105 9 -0.09711655988441947 1105 10-11 -0.09152692419148423 1105 12-13 -0.1687763805000415 1105 14-15 0.05565093472259264 1105 16-17 0.03281442575563176 1105 18-19 -0.08764090921311407 1105 20-21 0.0821989287387126 1105 22-23 -0.13705946213607234 1105 24-25 -0.08318477952050074 1105 26-27 0.031246781595328343 1105 28-29 0.1398235215652548 1105 30-31 -0.009929731862733604 1105 32-33 0.13217925738700842 1105 34-35 -0.034535132696547066 1105 36-37 0.044952789078003264 1105 38-39 0.03870338529703332 1105 40-41 0.15208755765867465 1105 42-43 0.306224645734531 1105 44-45 0.055996969819084086 1105 46-47 -0.17067117760872463 1105 48-49 -0.1024851424194324 1105 50-51 0.0785194686549957 1105 52-53 -0.015876320832092006 1105 54-55 0.020551043291014537 1105 56-57 -0.047946176136697716 1105 58-59 0.04764896185896106 1105 60-61 0.19569064109295908 1105 62-63 0.2262783582967245 1105 64-65 -0.11765135480938937 1105 66-67 0.08737489230716022 1105 68-69 0.10212977742472162 1105 70-71 0.008754702671367909 1105 72-73 0.0023013587193290164 1105 74-75 -0.16881954745084826 1105 76-77 0.1594839609432661 1105 78-79 0.200431297229958 1105 80-81 0.14872771648242633 1105 82-83 0.3782319691036342 1105 84-85 0.43052971273054297 1105 86-87 0.31319141847714604 1105 88-89 0.227781116641264 1105 90-91 0.2602333229136349 1105 92-93 0.3184268549761491 1105 94-95 0.2785230200140454 1105 96-97 0.27727034657607064 1105 98-99 0.40591017454986655 1105 100-101 0.3962796622808007 1106 1 -0.17954059249510124 1106 2 0.041523939526989295 1106 3 0.0162682914835095 1106 4 0.010855066137402503 1106 5 0.11942485318959228 1106 6 0.2836086339963231 1106 7 -0.06421318907914042 1106 8 0.07064816290137088 1106 9 -0.059367199560171 1106 10-11 0.25623449373755136 1106 12-13 0.0020962957221328793 1106 14-15 0.10553007563240158 1106 16-17 0.09700277346751562 1106 18-19 -0.09743417506914653 1106 20-21 0.03268774290440746 1106 22-23 -0.2145026838375017 1106 24-25 -0.049274888888078294 1106 26-27 0.2415472593708472 1106 28-29 0.1009349157187387 1106 30-31 -0.09001656926054835 1106 32-33 -0.12176210755649919 1106 34-35 0.07559806704078653 1106 36-37 0.43054564961737185 1106 38-39 0.3533332790995374 1106 40-41 0.6074178114910751 1106 42-43 0.6692533688738109 1106 44-45 0.873365298510528 1106 46-47 0.8850895836620154 1106 48-49 0.8232629385533272 1106 50-51 0.7113144554086119 1106 52-53 0.815246451624489 1106 54-55 1.0909592469909484 1106 56-57 0.8935305691025306 1106 58-59 1.0848294070279891 1106 60-61 0.9155678573146773 1106 62-63 1.261485998176262 1106 64-65 1.446132242319429 1106 66-67 1.0923465314621055 1106 68-69 1.0370569223197634 1106 70-71 1.0411276732576056 1106 72-73 0.6964967258818859 1106 74-75 0.934580074404554 1106 76-77 0.7478894134714018 1106 78-79 1.0399335389254993 1106 80-81 0.8831632167881125 1106 82-83 0.842166710855782 1106 84-85 1.035770525175348 1106 86-87 0.8802568918371989 1106 88-89 1.0834181883981628 1106 90-91 0.9160722038773343 1106 92-93 1.2119505626047022 1106 94-95 1.112505935576845 1106 96-97 1.3429913686986623 1106 98-99 1.1935661308934122 1106 100-101 1.4216077083827408 1107 1 -0.1907903998614131 1107 2 -0.23900849998603135 1107 3 -0.26534289668107647 1107 4 -0.3738151449891163 1107 5 -0.44023379991566003 1107 6 -0.26260418770193894 1107 7 -0.22043910986816684 1107 8 -0.2913297998047497 1107 9 -0.1813235872932566 1107 10-11 -0.29744288336215163 1107 12-13 -0.561337977663598 1107 14-15 -0.44618243785195233 1107 16-17 -0.5047463404175261 1107 18-19 -0.37582838064781754 1107 20-21 -0.31040248671494197 1107 22-23 -0.1932551880754403 1107 24-25 -0.3718881574964925 1107 26-27 -0.2788719115337628 1107 28-29 -0.487127574649584 1107 30-31 -0.37808098596574524 1107 32-33 -0.48157294127510397 1107 34-35 -0.37602198227343564 1107 36-37 -0.6677842163095775 1107 38-39 -0.7039752430148098 1107 40-41 -0.4538204378539703 1107 42-43 -0.36633570494342393 1107 44-45 -0.4324695758923127 1107 46-47 -0.49419651589106905 1107 48-49 -0.5820479722187528 1107 50-51 -0.5648644627606032 1107 52-53 -0.48412556255334493 1107 54-55 -0.11686360706976728 1107 56-57 -0.24482857714496475 1107 58-59 -0.423107100908517 1107 60-61 -0.8251568306187451 1107 62-63 -0.8310166986953718 1107 64-65 -0.627280456093267 1107 66-67 -0.7940670145388182 1107 68-69 -0.7509978627010412 1107 70-71 -0.9488457432934823 1107 72-73 -0.943355331445904 1107 74-75 -0.9954264751408353 1107 76-77 -1.2471213740151121 1107 78-79 -1.0409437148886518 1107 80-81 -0.8651030800218749 1107 82-83 -0.8577485303215973 1107 84-85 -0.6534920730678344 1107 86-87 -0.46260217735682474 1107 88-89 -0.5198142048827741 1107 90-91 -0.6333479687224468 1107 92-93 -0.4499484202894273 1107 94-95 -0.4237573561476111 1107 96-97 -0.5560589472205848 1107 98-99 -0.5489982087583059 1107 100-101 -0.5276622099405728 1108 1 0.008798845844339098 1108 2 -0.08174630874325928 1108 3 -0.308568908353692 1108 4 -0.10793817765583924 1108 5 -0.07293371416897543 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-0.7686042450725452 2316 56-57 -0.869842446770484 2316 58-59 -0.3888213866228014 2316 60-61 -0.4926464283996026 2316 62-63 -0.4322441605538998 2316 64-65 -0.4401375989504075 2316 66-67 -0.20253442091884466 2316 68-69 -0.12901450625166433 2316 70-71 -0.12399137436144514 2316 72-73 -0.7996937503085917 2316 74-75 -0.5365707192462388 2316 76-77 -0.16488836430637477 2316 78-79 -0.27256646107450067 2316 80-81 -0.5468367832118872 2316 82-83 -0.7281507494616797 2316 84-85 -0.41256280815798263 2316 86-87 -0.3318462827659765 2316 88-89 -0.4199945100145328 2316 90-91 -0.4101579548528207 2316 92-93 -0.4150732469191034 2316 94-95 -0.5418988263279161 2316 96-97 -0.9365721233648294 2316 98-99 -0.5760370437097606 2316 100-101 -0.930574831299797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 16.0 6 30.0 7 95.0 8 263.0 9 485.0 10 872.0 11 1340.0 12 2031.0 13 2864.0 14 3605.0 15 4616.0 16 5867.0 17 7655.0 18 6918.0 19 5174.0 20 4674.0 21 4842.0 22 4991.0 23 5420.0 24 6173.0 25 7417.0 26 8958.0 27 10993.0 28 13892.0 29 16996.0 30 21195.0 31 26413.0 32 33002.0 33 42148.0 34 55315.0 35 76072.0 36 112770.0 37 158280.0 38 139743.0 39 27284.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.44703719054238 19.44657382174117 14.480336168563547 19.626052819152903 2 15.239201914934952 24.856153830175376 37.326691177638565 22.577953077251106 3 24.03515204849907 26.006404615234185 29.3173706854405 20.641072650826246 4 12.51846520965115 17.54918863044044 41.09937184686308 28.83297431304533 5 12.49573879777281 40.43187513669448 34.05797189989138 13.01441416564133 6 27.8727223186805 40.111017300175575 16.48935585284526 15.52690452829866 7 28.206898321300145 33.26975639974536 21.25835898200337 17.26498629695112 8 24.19397603463212 32.242058279806116 19.435114261312194 24.12885142424957 9 23.092722538613515 22.414473547556238 22.608992083633193 31.883811830197047 10-11 26.033165899141164 25.38851778586099 27.924895317992597 20.653420997005245 12-13 29.537431988768752 23.683487062440207 26.685206205532293 20.09387474325875 14-15 22.319231932357262 27.043471089022415 29.088759710050187 21.548537268570136 16-17 19.750779234901906 29.23196946352228 29.112411431140806 21.904839870435016 18-19 19.58961720658186 32.38733625681334 28.950210830234923 19.07283570636987 20-21 22.704543621553057 26.60979502257025 27.78651504153561 22.89914631434109 22-23 20.44741706997902 28.407159552851734 28.310572302368804 22.83485107480044 24-25 24.106247197381332 28.099742067443685 28.183194429341018 19.610816305833968 26-27 23.076471907187994 26.009197377030752 31.890655442942233 19.02367527283902 28-29 20.270160455122852 31.166212590664994 29.09901097175523 19.464615982456923 30-31 22.18628968252756 27.573441583079216 28.188272930618695 22.051995803774528 32-33 22.391422687063585 28.68853110033523 28.856787979836056 20.063258232765126 34-35 23.76649504911781 26.31112691450709 29.97967349287839 19.94270454349671 36-37 23.183672421884165 25.77639220210395 31.827008623049547 19.21292675296234 38-39 21.32498507128804 28.55653260265667 30.799048229671737 19.319434096383553 40-41 20.238391256136193 29.148799032261017 29.23127678617795 21.381532925424835 42-43 20.455027302004712 29.425493968630644 27.101906099677826 23.017572629686814 44-45 22.860756400073317 27.02236207001894 27.459033420907925 22.65784810899982 46-47 24.27797436925092 28.74563778727065 27.640299514169246 19.33608832930918 48-49 23.44951543443504 29.029946353524227 27.775629312077577 19.74490889996316 50-51 20.327978822260086 29.369634771962343 30.819403316078258 19.482983089699314 52-53 22.183821602273166 30.789626578578073 26.36200183646875 20.66454998268001 54-55 20.027638570101104 29.143295966687095 27.783727578026724 23.04533788518508 56-57 23.903291165227934 26.678394059967346 28.092707915807175 21.325606858997546 58-59 25.781562654847683 26.769777275951633 27.132033025211268 20.31662704398942 60-61 21.516500233688397 28.356025036733374 30.90338130309538 19.224093426482852 62-63 20.6786917856593 27.830102224101232 29.53190257063189 21.959303419607576 64-65 20.22563430218657 31.771643263227993 27.9078285893545 20.09489384523094 66-67 22.274589237101356 26.785982781024654 26.938173781341263 24.001254200532728 68-69 20.795478574196142 29.306703302210234 27.48454132437979 22.413276799213836 70-71 23.833296771855768 28.45629701688896 26.984081397459107 20.72632481379616 72-73 22.459648351111277 26.807057414806394 30.057488117493246 20.67580611658908 74-75 20.617108743025515 31.379123064356797 27.58679886294421 20.416969329673478 76-77 22.066258892068433 27.306805991012016 27.098662554708675 23.528272562210876 78-79 21.972739119198998 28.70536169755219 27.8459065608293 21.47599262241951 80-81 23.19595123792953 27.13932770706591 28.373041813341022 21.29167924166354 82-83 22.01608089689397 26.85721163039474 30.40689764297134 20.719809829739948 84-85 20.311024223355837 28.98720102636161 28.738125057274644 21.963649693007913 86-87 20.1197014307107 28.729992992163076 27.15582631073128 23.994479266394947 88-89 20.88272668401696 28.445682587392934 26.359432083379154 24.312158645210953 90-91 22.44541623443191 25.31160612936374 27.860172209657375 24.382805426546973 92-93 22.7937389612873 28.09812487743249 27.79388252946355 21.314253631816662 94-95 21.49613332306944 29.001543249474892 27.89597545463543 21.606347972820235 96-97 18.588633229525815 29.817747236331044 30.513178178058915 21.080441356084226 98-99 21.213539496307664 31.466228857520985 25.1178333090966 22.20239833707475 100-101 18.744050026824528 29.66034895103271 26.256567120585718 25.339033901557045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 100.0 1 82.5 2 103.5 3 152.5 4 143.5 5 131.0 6 179.5 7 307.0 8 465.5 9 577.5 10 601.0 11 596.5 12 664.0 13 752.0 14 811.5 15 928.5 16 1060.5 17 1141.0 18 1203.0 19 1307.5 20 1449.0 21 1561.5 22 1782.5 23 2188.0 24 2618.5 25 3069.0 26 3820.5 27 4895.5 28 6456.5 29 8467.5 30 10006.5 31 11352.0 32 13084.0 33 14839.5 34 16667.5 35 18725.0 36 20330.0 37 21954.5 38 23696.0 39 24367.5 40 24800.0 41 25538.0 42 26484.5 43 27776.0 44 28469.0 45 29127.5 46 32294.5 47 46942.5 48 82198.0 49 79131.5 50 44857.0 51 31362.0 52 22677.0 53 17777.5 54 15269.0 55 13059.5 56 10799.5 57 8647.5 58 6687.0 59 5486.5 60 4528.5 61 3568.5 62 2741.0 63 1986.5 64 1350.0 65 905.5 66 584.0 67 354.5 68 203.0 69 107.5 70 51.0 71 31.5 72 14.5 73 11.0 74 11.0 75 3.5 76 2.5 77 1.5 78 1.0 79 0.5 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06206998513008427 2 0.0026880702221690035 3 0.033112137736718186 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0016494976363309796 16-17 0.0 18-19 0.0 20-21 0.011851945979563336 22-23 0.0 24-25 0.0 26-27 0.005559417959485895 28-29 0.012523963535105586 30-31 0.009347153272542218 32-33 0.004826307898894348 34-35 0.00507067791909153 36-37 0.01942741660567598 38-39 0.04600265630211954 40-41 0.003482272787809846 42-43 0.010324633353330948 44-45 0.009896985817985877 46-47 0.004765215393845053 48-49 0.002138237676725344 50-51 0.0029935327474154816 52-53 0.0015273126262323887 54-55 0.0011607575959366153 56-57 0.01454001620173234 58-59 0.010569003373528129 60-61 0.004093197838302801 62-63 0.004032105333253506 64-65 0.09536540038195034 66-67 0.04643030383746461 68-69 0.03445617284780269 70-71 0.021321284262204147 72-73 1.221850100985911E-4 74-75 1.8327751514788662E-4 76-77 6.109250504929554E-4 78-79 6.720175555422509E-4 80-81 9.774800807887288E-4 82-83 7.94202565640842E-4 84-85 7.331100605915465E-4 86-87 0.0075143781210633516 88-89 1.221850100985911E-4 90-91 0.0012218501009859108 92-93 5.498325454436598E-4 94-95 0.0034211802827605504 96-97 0.010935558403823903 98-99 0.013929091151239383 100-101 0.018327751514788663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 818431.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.27961673051157 #Duplication Level Percentage of deduplicated Percentage of total 1 84.14842640034071 45.67544333486156 2 9.451508215048406 10.26048486876218 3 2.5264176592194105 4.113989467308775 4 1.0942312682953967 2.3757781543046286 5 0.6343128383829928 1.721512887733589 6 0.397047840907328 1.2930962776876136 7 0.2916736684648262 1.1082354454257144 8 0.21329918222533886 0.9262238288098353 9 0.15395311407511192 0.7520864423819221 >10 0.9725802407586723 9.62907109364769 >50 0.07083514018191454 2.6986772081852046 >100 0.03575683302928421 3.843162720054823 >500 0.002942017907237136 1.09118501696551 >1k 0.006110344884261744 8.07191924460437 >5k 6.789272093624159E-4 2.3935314708513973 >10k+ 2.26309069787472E-4 4.045602538415187 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 32934 4.024041122586999 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7761 0.9482778633751654 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5927 0.7241905548543494 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5797 0.7083065035415326 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4701 0.5743917324734767 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4270 0.5217299931209839 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4191 0.5120773773231952 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3873 0.4732225441118433 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3676 0.4491520971224208 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3472 0.4242263550623082 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3274 0.4000337230627872 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3195 0.39038110726499853 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3032 0.3704649506189282 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2703 0.3302660822964917 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2416 0.2951989843981961 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2344 0.28640166367109754 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2274 0.2778487129641961 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2255 0.2755271977723229 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2180 0.26636332201492857 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2140 0.26147592161098493 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1879 0.22958563397525267 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1875 0.2290968939348583 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1695 0.20710359211711188 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1613 0.19708442128902742 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1544 0.18865365559222463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1381 0.16873749894615428 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1329 0.16238387842102756 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1226 0.14979882238087266 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1082 0.13220418092667555 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1048 0.12804989058332344 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1043 0.1274389655328305 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 948 0.11583138957346435 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 933 0.11399861442198547 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 917 0.11204365426040802 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.221850100985911E-4 0.0 6 0.0 0.0 0.0 1.221850100985911E-4 0.0 7 0.0 0.0 0.0 1.221850100985911E-4 0.0 8 0.0 0.0 0.0 1.221850100985911E-4 0.0 9 0.0 0.0 0.0 2.443700201971822E-4 0.0 10-11 0.0 0.0 0.0 3.054625252464777E-4 0.0 12-13 0.0 0.0 0.0 4.887400403943644E-4 0.0 14-15 0.0 0.0 0.0 6.109250504929554E-4 0.0 16-17 0.0 0.0 0.0 6.109250504929554E-4 0.0 18-19 0.0 0.0 0.0 9.774800807887288E-4 0.0 20-21 0.0 0.0 0.0 0.0017105901413802752 0.0 22-23 0.0 0.0 0.0 0.003115717757514073 0.0 24-25 0.0 0.0 0.0 0.004643030383746461 0.0 26-27 0.0 0.0 0.0 0.005498325454436599 0.0 28-29 0.0 0.0 0.0 0.009896985817985877 0.0 30-31 0.0 0.0 0.0 0.01985506414102105 0.0 32-33 0.0 0.0 0.0 0.035006005393246345 0.0 34-35 0.0 0.0 0.0 0.04716341389805616 0.0 36-37 0.0 0.0 0.0 0.0588931748675209 0.0 38-39 0.0 0.0 0.0 0.07373865359449971 0.0 40-41 0.0 0.0 0.0 0.08846194731137995 0.0 42-43 0.0 0.0 0.0 0.13092123832064034 0.0 44-45 0.0 0.0 0.0 0.1798563348651261 0.0 46-47 0.0 0.0 0.0 0.21858898306637944 0.0 48-49 0.0 0.0 0.0 0.2702121498330342 0.0 50-51 0.0 1.221850100985911E-4 0.0 0.3327708750035128 0.0 52-53 0.0 2.443700201971822E-4 0.0 0.47994271966726576 0.0 54-55 0.0 2.443700201971822E-4 0.0 0.5596684387565964 0.0 56-57 0.0 2.443700201971822E-4 0.0 0.7606627803687789 0.0 58-59 0.0 2.443700201971822E-4 0.0 1.015785081454637 0.0 60-61 0.0 2.443700201971822E-4 0.0 1.216290683026425 0.0 62-63 0.0 2.443700201971822E-4 0.0 1.6393562804927966 0.0 64-65 0.0 2.443700201971822E-4 0.0 1.8091934445298383 0.0 66-67 0.0 2.443700201971822E-4 0.0 2.3425310136101882 0.0 68-69 0.0 2.443700201971822E-4 0.0 2.8587015887716865 0.0 70-71 0.0 2.443700201971822E-4 0.0 3.1268365934330444 0.0 72-73 0.0 4.276475353450688E-4 0.0 3.688093437320923 0.0 74-75 0.0 5.4983254544366E-4 0.0 4.151357903109731 0.0 76-77 0.0 6.109250504929554E-4 0.0 4.34398257153016 0.0 78-79 0.0 6.109250504929554E-4 0.0 4.59378982467673 0.0 80-81 0.0 6.109250504929554E-4 0.0 5.111304434949312 0.0 82-83 0.0 6.109250504929554E-4 0.0 5.487389896032775 0.0 84-85 0.0 6.109250504929554E-4 0.0 6.310244846541737 0.0 86-87 0.0 6.109250504929554E-4 0.0 8.007394636811167 0.0 88-89 0.0 6.109250504929554E-4 0.0 10.016299480347152 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 770 0.0 41.32771 2 ACCTGGG 830 0.0 38.340164 3 GCGTATC 50 0.0016181495 37.987537 9 GTACACG 105 4.633148E-8 36.209576 1 GACGCTT 80 1.0796533E-5 35.613316 5 TATAACG 100 1.2994351E-6 33.24722 2 ACACGGG 90 2.4134986E-5 31.664019 3 TACACGG 110 2.733963E-6 30.224745 2 GTACCTG 1120 0.0 28.854506 1 CGACCAT 85 6.4178876E-4 27.932014 6 GGGTACG 70 0.008368614 27.15718 1 TGCGCGA 35 0.008406033 27.133955 12-13 GAGCGTA 70 0.008403621 27.133955 7 GTACGCA 285 0.0 26.664434 3 TACGCAG 285 0.0 26.657919 4 GAGTACG 325 0.0 26.321575 1 ATGGGCG 110 9.4945106E-5 25.900593 5 AGTACGC 295 0.0 25.76056 2 TATACTG 485 0.0 25.455564 5 TAACGCA 150 1.0625208E-6 25.325024 4 >>END_MODULE