##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003821_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1378150 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.858839748938795 31.0 31.0 34.0 30.0 34.0 2 32.035215324892064 33.0 31.0 34.0 30.0 34.0 3 32.10371004607626 33.0 31.0 34.0 30.0 34.0 4 35.78960563073686 37.0 35.0 37.0 35.0 37.0 5 35.68890831912346 37.0 35.0 37.0 33.0 37.0 6 35.78163480027573 37.0 35.0 37.0 33.0 37.0 7 35.74757827522403 37.0 35.0 37.0 33.0 37.0 8 35.75355657947248 37.0 35.0 37.0 33.0 37.0 9 37.36944091717157 39.0 37.0 39.0 34.0 39.0 10-11 37.270980299677106 39.0 37.0 39.0 34.0 39.0 12-13 37.248464971156984 39.0 37.0 39.0 33.5 39.0 14-15 38.35406087871422 40.0 38.0 41.0 33.5 41.0 16-17 38.41950186844683 40.0 38.0 41.0 34.0 41.0 18-19 38.46338025614048 40.0 38.0 41.0 34.0 41.0 20-21 38.39675398178718 40.0 38.0 41.0 34.0 41.0 22-23 38.30117186082792 40.0 38.0 41.0 34.0 41.0 24-25 38.15242571563328 40.0 37.5 41.0 33.0 41.0 26-27 37.92715633276494 40.0 37.0 41.0 33.0 41.0 28-29 37.80553785872365 40.0 37.0 41.0 33.0 41.0 30-31 37.645688060080545 39.5 37.0 41.0 33.0 41.0 32-33 37.37012661901825 39.0 36.0 41.0 32.0 41.0 34-35 37.26620469469942 39.0 36.0 41.0 32.0 41.0 36-37 37.08090519899866 39.0 36.0 41.0 31.5 41.0 38-39 36.8754333708232 39.0 36.0 40.0 31.0 41.0 40-41 36.977516235533145 39.0 36.0 41.0 31.0 41.0 42-43 36.851320610963974 39.0 36.0 41.0 31.0 41.0 44-45 36.605745383303706 39.0 36.0 41.0 30.0 41.0 46-47 36.4228857526394 39.0 36.0 41.0 30.0 41.0 48-49 36.15358161303196 39.0 35.0 41.0 29.0 41.0 50-51 35.86894097159235 39.0 35.0 40.5 28.0 41.0 52-53 35.75644523455357 39.0 35.0 40.0 28.0 41.0 54-55 35.596578746870804 39.0 35.0 40.0 28.0 41.0 56-57 35.27250408155861 38.5 34.5 40.0 27.0 41.0 58-59 35.004135616587455 38.0 34.0 40.0 26.0 41.0 60-61 34.554494068134815 38.0 34.0 40.0 26.0 41.0 62-63 34.39042484490077 37.5 34.0 40.0 26.0 41.0 64-65 33.9657123680296 37.0 33.0 39.5 24.5 41.0 66-67 33.55764611979828 36.5 33.0 39.0 23.5 41.0 68-69 33.17629793563836 36.0 32.5 39.0 23.0 40.0 70-71 32.65648042665893 35.0 32.0 38.5 22.0 40.0 72-73 32.20690672278054 35.0 31.5 37.5 21.5 40.0 74-75 31.852314697239052 35.0 31.0 37.0 20.0 39.0 76-77 30.890540579762725 34.0 30.5 36.0 20.0 39.0 78-79 31.319563908137724 34.5 31.0 36.0 20.0 39.0 80-81 31.03486376664369 34.0 31.0 36.0 19.5 37.5 82-83 30.685805246163333 34.0 31.0 35.0 17.5 37.0 84-85 30.37579472481225 34.0 31.0 35.0 17.5 37.0 86-87 30.191312629249357 34.0 31.0 35.0 12.5 36.0 88-89 29.862309255160902 34.0 30.0 35.0 7.5 36.0 90-91 29.53200486158981 34.0 30.0 35.0 4.5 36.0 92-93 29.26639770707107 34.0 30.0 35.0 2.0 35.0 94-95 28.89848166019664 34.0 29.0 35.0 2.0 35.0 96-97 28.519410078728733 34.0 29.0 35.0 2.0 35.0 98-99 28.247480680622573 34.0 29.0 35.0 2.0 35.0 100-101 27.316307731379023 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 9.9020525323823E-4 1101 2 0.05594317930270165 1101 3 0.07390153547280676 1101 4 0.04234154270852741 1101 5 -0.009690412097093315 1101 6 -0.02358208145925289 1101 7 -0.009471828681611782 1101 8 -0.05463893778021145 1101 9 -0.03204630339208592 1101 10-11 0.10645946653027494 1101 12-13 0.09506190966206418 1101 14-15 0.263993917905772 1101 16-17 0.0607925023253344 1101 18-19 0.03713703093419696 1101 20-21 -0.05424712215848615 1101 22-23 -0.0670986788359258 1101 24-25 0.09677583719629723 1101 26-27 -0.0026424152666422174 1101 28-29 0.07149334745275127 1101 30-31 0.07781026950454617 1101 32-33 0.17340809055982476 1101 34-35 0.1696161279034385 1101 36-37 0.29856982806830246 1101 38-39 0.024165237677884477 1101 40-41 0.226571591626481 1101 42-43 0.25611474136237433 1101 44-45 0.2229307459465275 1101 46-47 0.2675164004715711 1101 48-49 0.27235459735186396 1101 50-51 0.29846932369462564 1101 52-53 0.06635541375626275 1101 54-55 -0.04449910933352896 1101 56-57 0.027317228646055014 1101 58-59 0.023035262681226243 1101 60-61 0.0022058193720440045 1101 62-63 -0.10684968270501116 1101 64-65 -0.1336257801538494 1101 66-67 -0.1299301499959995 1101 68-69 0.030338313970865727 1101 70-71 -0.0014911355136533189 1101 72-73 -0.12122329197055848 1101 74-75 -0.10961136708650443 1101 76-77 -0.18025605442979398 1101 78-79 -0.1247618910486068 1101 80-81 -0.058552081309702686 1101 82-83 0.06411190675147793 1101 84-85 -0.007781497609538945 1101 86-87 -0.1485909261016367 1101 88-89 -0.01746426104606158 1101 90-91 -0.1452687107612327 1101 92-93 0.040027787224481415 1101 94-95 0.0028148816556345935 1101 96-97 0.11785932889343798 1101 98-99 -0.10164770879397622 1101 100-101 -0.21328512044823 1102 1 -2.103074865118515E-4 1102 2 0.0521490646459597 1102 3 -0.0027845549663538804 1102 4 0.038441770172006784 1102 5 0.014283161471688288 1102 6 -0.02190311794311839 1102 7 -0.08523570524928203 1102 8 0.03543420538222364 1102 9 0.05431446045457733 1102 10-11 -0.014449829170246176 1102 12-13 0.013509177409098072 1102 14-15 0.022805286693632354 1102 16-17 -0.014330648910963362 1102 18-19 -0.04219868003368532 1102 20-21 -0.09030496685735301 1102 22-23 0.011938997169167465 1102 24-25 -0.015269161099219275 1102 26-27 0.004510822549306681 1102 28-29 0.10190527363576507 1102 30-31 -0.008735894017966928 1102 32-33 -0.09524162167196693 1102 34-35 -0.010760014148594621 1102 36-37 -0.11862569283500335 1102 38-39 -0.004899650215115514 1102 40-41 0.051378865673115115 1102 42-43 0.07430404439913474 1102 44-45 0.24617667562622358 1102 46-47 -0.12113414904908382 1102 48-49 0.11239016219290932 1102 50-51 0.14209087710468538 1102 52-53 0.1388638314996129 1102 54-55 -0.022146885833222996 1102 56-57 0.008142516364429753 1102 58-59 0.04932633219812743 1102 60-61 -0.13827829307187045 1102 62-63 -0.17234740109287117 1102 64-65 -0.2918402815981693 1102 66-67 -0.11480153644667013 1102 68-69 -0.07573422315392264 1102 70-71 -0.01819185857239347 1102 72-73 0.017746922475062377 1102 74-75 -0.034906860279544816 1102 76-77 0.0558386708497558 1102 78-79 0.12531117237204015 1102 80-81 0.08789273039001344 1102 82-83 0.019678581857487387 1102 84-85 -0.025316519209599875 1102 86-87 -0.03703414695251794 1102 88-89 -0.06475234223915649 1102 90-91 -0.19868873671718745 1102 92-93 -0.202637263341785 1102 94-95 -0.1683580603581234 1102 96-97 -0.25223829500039585 1102 98-99 -0.24768882765111755 1102 100-101 -0.21629696808557597 1103 1 -0.04628729906230333 1103 2 0.05501919290416879 1103 3 -0.007655984382687109 1103 4 -0.06975719206692332 1103 5 -0.017526073085804228 1103 6 0.009248873486896514 1103 7 -0.03828322251128924 1103 8 -0.08335020304481588 1103 9 0.06192315339042409 1103 10-11 -0.029544762900314936 1103 12-13 -0.05401110008244814 1103 14-15 -0.0607572626100108 1103 16-17 -0.08738355573321854 1103 18-19 -0.1474692034707843 1103 20-21 -0.130764570562242 1103 22-23 -0.062451511873554466 1103 24-25 -0.018025167293465927 1103 26-27 -0.18095356678519892 1103 28-29 -0.15869641620378871 1103 30-31 -0.2611918091644654 1103 32-33 -0.09446005213539621 1103 34-35 0.039112160809686713 1103 36-37 0.0032768719126039514 1103 38-39 0.143755791949566 1103 40-41 -0.04287637956853274 1103 42-43 0.30786338662289126 1103 44-45 0.10756501634971727 1103 46-47 0.02976312526620717 1103 48-49 -0.004805075668294023 1103 50-51 0.2646563077821966 1103 52-53 0.13451032466186774 1103 54-55 0.0810663166734571 1103 56-57 0.033100053793546635 1103 58-59 -0.08133635047006038 1103 60-61 -0.14931273044592075 1103 62-63 -0.15158572378174995 1103 64-65 -0.007262129226056402 1103 66-67 -0.01663948478186228 1103 68-69 -0.13206220829972892 1103 70-71 -0.10442690504173413 1103 72-73 0.07133535333000651 1103 74-75 -0.043716958666092154 1103 76-77 -0.10959292296899648 1103 78-79 0.1330947969387708 1103 80-81 0.13263940856329626 1103 82-83 0.22708876904265907 1103 84-85 0.08676530047920394 1103 86-87 0.03956466567820982 1103 88-89 0.06252104008058623 1103 90-91 -0.07420947069698869 1103 92-93 -0.08443762636656515 1103 94-95 -0.0073505707852952185 1103 96-97 0.08903832272151035 1103 98-99 -0.04860059630153302 1103 100-101 0.15805047445563147 1104 1 -0.06543313732531075 1104 2 -0.11444954246874062 1104 3 0.003011969612032317 1104 4 0.0057164450551070445 1104 5 -0.06656169971691384 1104 6 -0.0922966110487593 1104 7 -0.11257028400077473 1104 8 -0.18545443171019826 1104 9 -0.10676057670593764 1104 10-11 -0.012735373235443603 1104 12-13 -0.125890898416408 1104 14-15 -0.1693388642971243 1104 16-17 -0.059647134424601234 1104 18-19 -0.052131219405779916 1104 20-21 -0.08074747143739813 1104 22-23 -0.18321883078259305 1104 24-25 -0.07614491989158267 1104 26-27 -0.21192414074581478 1104 28-29 0.015794107599404583 1104 30-31 0.06738212382423825 1104 32-33 -5.003611632119487E-4 1104 34-35 -0.11083334652479948 1104 36-37 0.0444369698094107 1104 38-39 0.022963428867790014 1104 40-41 0.11365013184728667 1104 42-43 0.13658240309504066 1104 44-45 0.36622727754490114 1104 46-47 0.35686463361628284 1104 48-49 0.1286393444036591 1104 50-51 0.26990306197104985 1104 52-53 0.2252000239072629 1104 54-55 0.045964749599122 1104 56-57 0.1145834287808114 1104 58-59 0.18548426589294564 1104 60-61 0.13019794096243942 1104 62-63 -0.24235832787483247 1104 64-65 0.03227571079176528 1104 66-67 -0.007542404932408431 1104 68-69 -0.04083708191301838 1104 70-71 -0.2983335714550819 1104 72-73 -0.28860208805367904 1104 74-75 -0.26036200674226606 1104 76-77 -0.24352699979760928 1104 78-79 -0.2222625834062164 1104 80-81 -0.17123276597904535 1104 82-83 0.040428936268646964 1104 84-85 0.039705140921913795 1104 86-87 0.02854825224555313 1104 88-89 -0.010436521273316401 1104 90-91 -0.2705968055997552 1104 92-93 -0.1656817863245621 1104 94-95 -0.2837531739975141 1104 96-97 -0.2851630704253658 1104 98-99 -0.1973862993605522 1104 100-101 -0.4737103726937413 1105 1 0.0067871874488076855 1105 2 -0.027586438174644456 1105 3 0.043205095330044685 1105 4 -0.1142140048470921 1105 5 -0.09596835044501262 1105 6 -0.0043685208820249954 1105 7 -0.023797656932124767 1105 8 0.07912159213554304 1105 9 -0.00219945645257269 1105 10-11 0.029673109392561514 1105 12-13 0.09793777236944123 1105 14-15 0.014071574427383382 1105 16-17 0.09500868573871912 1105 18-19 -0.05590679614375915 1105 20-21 -0.05883142834117905 1105 22-23 0.034637535845782 1105 24-25 -0.02585461033155667 1105 26-27 -0.04631418017823563 1105 28-29 -0.007134137813082475 1105 30-31 -0.010183312261084154 1105 32-33 0.10091392765598783 1105 34-35 0.14205577695767602 1105 36-37 0.029086269651060093 1105 38-39 0.03159043541915452 1105 40-41 0.14174495595384684 1105 42-43 0.3103241362586431 1105 44-45 0.4492527067994061 1105 46-47 0.26906850234047397 1105 48-49 0.2818420643092736 1105 50-51 0.3895835662092608 1105 52-53 0.3967124280430099 1105 54-55 0.3323139904473891 1105 56-57 0.27383297080583446 1105 58-59 0.4017974897262704 1105 60-61 0.3588889087644631 1105 62-63 0.28813504313146865 1105 64-65 0.06900219882231795 1105 66-67 0.2947769666327815 1105 68-69 0.05739466894859646 1105 70-71 0.21115504903114868 1105 72-73 0.2760469012964677 1105 74-75 0.44143108171676104 1105 76-77 0.5118569229521022 1105 78-79 0.4251789665673904 1105 80-81 0.384006778219419 1105 82-83 0.6608266758904691 1105 84-85 0.44486550978582784 1105 86-87 0.45919221163326185 1105 88-89 0.588802774531942 1105 90-91 0.4274486503570216 1105 92-93 0.3934575312151054 1105 94-95 0.4151771827572297 1105 96-97 0.398635482684206 1105 98-99 0.48092431634410815 1105 100-101 0.48181171877705964 1106 1 0.11168339630345514 1106 2 0.035079380255645276 1106 3 -0.08677299189693244 1106 4 -0.15308914916508343 1106 5 -0.004493126015809423 1106 6 0.023083706916565916 1106 7 0.10252123282070613 1106 8 -0.017414775999043286 1106 9 0.02129669114055588 1106 10-11 0.040498726131090734 1106 12-13 -0.039352819275428885 1106 14-15 0.10276057798525784 1106 16-17 -0.12865593102337414 1106 18-19 -0.08034425049214633 1106 20-21 0.06524434744166996 1106 22-23 -0.02515370375743231 1106 24-25 -0.018388952123693514 1106 26-27 0.06630451400898352 1106 28-29 0.13617068535319277 1106 30-31 0.12591974845850018 1106 32-33 0.0993670743773194 1106 34-35 0.2635196915685398 1106 36-37 0.12002427770930524 1106 38-39 0.252845825254461 1106 40-41 0.10743143836473479 1106 42-43 0.018815924859289623 1106 44-45 0.18913630991903574 1106 46-47 0.04873085713889225 1106 48-49 0.01727478267123672 1106 50-51 0.005213499528458954 1106 52-53 0.13912071248493874 1106 54-55 0.09833623309010875 1106 56-57 -0.10010850088427503 1106 58-59 0.029048261385746343 1106 60-61 -0.016036281499658855 1106 62-63 0.04532738072955311 1106 64-65 0.14047671638788017 1106 66-67 0.1095621978950021 1106 68-69 -0.059764446058117926 1106 70-71 0.2342223660123679 1106 72-73 0.11103193934928868 1106 74-75 0.1465805833778937 1106 76-77 -0.0012318355597109587 1106 78-79 -0.03499397940466409 1106 80-81 0.010487068504758668 1106 82-83 0.16763495090755143 1106 84-85 0.1232979218402086 1106 86-87 0.04511502212311669 1106 88-89 0.06081450846846437 1106 90-91 -0.04462316698941038 1106 92-93 -0.06871230455691091 1106 94-95 -0.12193315865791376 1106 96-97 -0.13949745480548614 1106 98-99 0.04891988666526004 1106 100-101 0.2871968416535218 1107 1 -0.11893178162637597 1107 2 -0.013247548228743966 1107 3 -0.03157256698987965 1107 4 -0.04731853648660689 1107 5 -0.019052441165619882 1107 6 -0.023502977246273815 1107 7 0.02063914452752158 1107 8 -0.09802214313747726 1107 9 -0.0533276097936195 1107 10-11 -0.007193966092486903 1107 12-13 -0.01657186510914954 1107 14-15 -0.07991664887136807 1107 16-17 0.03035147360686352 1107 18-19 0.0037514160807958774 1107 20-21 -0.1125573834297029 1107 22-23 -0.09132047977518454 1107 24-25 -0.004131133521504182 1107 26-27 -0.024389709129700066 1107 28-29 0.011016812762825623 1107 30-31 0.1250277560818347 1107 32-33 -0.013661325660173418 1107 34-35 -0.11852642334381613 1107 36-37 0.024401445203913852 1107 38-39 0.13925504823693302 1107 40-41 0.09252063760239793 1107 42-43 0.026926650638024796 1107 44-45 0.3505838057793156 1107 46-47 0.014889822365255156 1107 48-49 0.07818489608392554 1107 50-51 -0.12444407537703484 1107 52-53 -0.1923224093321565 1107 54-55 -0.06154129370090544 1107 56-57 -0.07061651475623876 1107 58-59 -0.018311691124637264 1107 60-61 -0.10479967700690196 1107 62-63 -0.013061409222650866 1107 64-65 -0.15481025208776344 1107 66-67 -0.15315064617036 1107 68-69 -0.19440061939520348 1107 70-71 -0.20830114446970782 1107 72-73 -0.2419642802276769 1107 74-75 -0.299014130278497 1107 76-77 -0.11828904179584754 1107 78-79 0.026391030124500503 1107 80-81 -0.014155978328748375 1107 82-83 0.0744115935169809 1107 84-85 0.15505816178772136 1107 86-87 0.25088875911815123 1107 88-89 0.1530521439856969 1107 90-91 -4.000283009979455E-4 1107 92-93 -0.1149303193661666 1107 94-95 -0.01661088540518918 1107 96-97 0.05909035411131214 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-0.16987356934186693 2316 80-81 -0.34543052565367915 2316 82-83 -0.5063913465977237 2316 84-85 -0.5522942964054884 2316 86-87 -0.7181602364602782 2316 88-89 -0.6227600382731886 2316 90-91 -0.6197912953568938 2316 92-93 -0.5506757030440816 2316 94-95 -0.588059717040732 2316 96-97 -0.693018646189973 2316 98-99 -0.6475064842527942 2316 100-101 -1.063593001164481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 13.0 5 17.0 6 53.0 7 152.0 8 348.0 9 728.0 10 1317.0 11 2454.0 12 4122.0 13 6041.0 14 6089.0 15 7407.0 16 9019.0 17 11850.0 18 10856.0 19 7791.0 20 6908.0 21 7104.0 22 7357.0 23 7915.0 24 9359.0 25 11052.0 26 13753.0 27 17092.0 28 21711.0 29 27295.0 30 33924.0 31 42208.0 32 53373.0 33 68445.0 34 91677.0 35 127909.0 36 190782.0 37 270656.0 38 251455.0 39 49896.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.69663455639842 19.381592387623137 13.478472036188846 19.443301019789597 2 15.14152114000601 25.33034660815123 36.69122224979645 22.8369100020463 3 24.72844786286073 26.021024421545313 29.33169574546542 19.91883197012854 4 12.307586256938649 17.313572542901717 41.42582447483946 28.953016725320175 5 11.546928853898342 40.480716903094724 33.53531908718209 14.437035155824837 6 26.41316257301455 39.53793128469325 17.666654573159672 16.382251569132535 7 27.186808402568662 32.82037514058702 22.64325363712223 17.34956281972209 8 24.007401226281612 30.447266262743533 20.15179769981497 25.39353481115989 9 22.60559445633639 22.366288139897687 24.46148822697094 30.56662917679498 10-11 25.21369226862098 25.17541631897834 28.43173819976055 21.179153212640134 12-13 29.672604578601746 23.245836810216595 26.955919166999237 20.12563944418242 14-15 22.45664675920534 26.99255111921874 29.945763324339236 20.605038797236684 16-17 19.6157892827341 29.16231905090157 29.657947248122486 21.563944418241846 18-19 18.879947756049777 32.5801980916446 28.952545078547328 19.5873090737583 20-21 22.294899594196842 27.286815461158426 27.454521309019974 22.96376363562476 22-23 20.03247106628451 28.450785473279396 28.463265972499364 23.05347748793673 24-25 23.665493596488048 28.068932989877737 28.29180423030875 19.973769183325473 26-27 22.71638138970565 25.33517745030027 32.676971371680516 19.27146978831356 28-29 19.655949404751105 31.723736298027205 29.27510622317207 19.345208074049616 30-31 21.762421495408827 27.535940035899216 28.237197524427177 22.46444094426478 32-33 22.026709508695394 28.36419497767193 29.375162362871404 20.233933150761274 34-35 23.950261269112627 25.927793794414118 30.053821219507416 20.06812371696584 36-37 23.075934939347196 25.792166028550312 32.04708525793363 19.08481377416886 38-39 21.034096023461434 28.88892115158466 31.18886017508965 18.888122649864254 40-41 20.41864224998077 29.58157544515026 29.00174446364999 20.998037841218977 42-43 19.66571527888144 29.90833730652031 26.9572935013175 23.46865391328075 44-45 22.419746241665482 26.733304112324785 27.321401098980846 23.52554854702889 46-47 24.067076248110133 29.378730524895808 27.52367707527937 19.030516151714693 48-49 23.143052023205275 28.631701538673642 28.37454240694852 19.850704031172565 50-51 19.26274547365572 29.326734519654035 31.58189759793459 19.828622408755656 52-53 21.729803114996706 30.851303514629684 26.850511142014287 20.56838222835932 54-55 19.88770294250073 28.77274684799444 27.98820576135569 23.351344448149142 56-57 23.136246040414083 26.8131365726042 28.43133270681466 21.619284680167056 58-59 26.074665743604147 26.514462945607164 27.12395996724289 20.286911343545803 60-61 21.845102100719448 28.673936950479128 30.901790213961426 18.57917073484 62-63 20.422386759487065 28.514008868177203 29.838901916334652 21.224702456001076 64-65 19.74318906794485 32.524156580094846 27.535451555773072 20.197202796187227 66-67 22.115769172980905 27.647533183761137 26.35749110659959 23.879206536658376 68-69 20.62315649205114 29.421044151289582 27.033048146040667 22.922751210618607 70-71 24.157282781488433 28.713255344850296 26.494723821128446 20.63473805253282 72-73 22.918293245718164 26.27820088988991 30.677438127109713 20.12606773728221 74-75 20.10599037546149 32.35739516335013 27.47256455058397 20.064049910604407 76-77 21.945613692648287 27.821935491361188 26.790001893855564 23.44244892213496 78-79 22.402589863744087 29.028938962748395 27.790641312723942 20.777829860783577 80-81 24.12648633195007 27.43155453614688 27.88252435708683 20.55943477481622 82-83 22.555593892209185 26.677739549966383 30.471508186931455 20.295158370892974 84-85 20.735724312179073 29.318461098068155 29.36947193623863 20.576342653514143 86-87 20.953422768873526 29.969105735303454 27.114424882309724 21.963046613513292 88-89 20.260131807371852 29.697982255164995 26.024246316159918 24.017639621303235 90-91 22.213575283153997 26.43855436723635 26.825634896965976 24.522235452643677 92-93 23.472845897397477 29.248042747362668 27.160923372638624 20.11818798260123 94-95 22.35873769147601 28.605662828076134 27.895995239857484 21.139604240590373 96-97 18.524243100417433 29.48821633114368 31.07771906049936 20.909821507939533 98-99 21.310480301178767 30.704755698623853 26.48085848719787 21.50390551299951 100-101 19.46380586156875 28.780640727649022 27.17417728389937 24.581376126882855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 269.0 1 207.5 2 436.0 3 625.0 4 444.5 5 354.5 6 445.5 7 680.5 8 964.0 9 1154.5 10 1167.5 11 1140.5 12 1175.5 13 1312.0 14 1495.5 15 1583.0 16 1700.5 17 1864.0 18 2064.0 19 2246.5 20 2430.5 21 2789.0 22 3163.0 23 3691.0 24 4502.5 25 5532.5 26 6805.5 27 8811.5 28 11515.5 29 14431.5 30 17500.0 31 20621.5 32 23827.0 33 26687.0 34 29582.5 35 32637.5 36 35056.5 37 37792.5 38 40215.0 39 41171.5 40 42085.0 41 43011.0 42 43995.0 43 45247.0 44 45270.5 45 45633.0 46 49112.0 47 77246.0 48 144998.5 49 143536.0 50 77465.5 51 46967.5 52 34444.5 53 27504.5 54 23129.5 55 19951.5 56 17106.0 57 14262.0 58 11760.0 59 9625.5 60 7679.5 61 5775.5 62 4237.5 63 3036.0 64 2019.0 65 1280.0 66 761.5 67 431.5 68 247.5 69 159.5 70 94.0 71 47.5 72 26.5 73 17.0 74 11.0 75 7.0 76 4.0 77 1.5 78 1.0 79 1.5 80 1.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05144577876138301 2 0.0037731741827812647 3 0.03729637557595327 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0017051844864492253 16-17 0.0 18-19 0.0 20-21 0.010303667960671914 22-23 0.0 24-25 0.0 26-27 0.0053695171062656455 28-29 0.011246961506367231 30-31 0.00910641076805863 32-33 0.004643906686500018 34-35 0.005006711896382832 36-37 0.019337517686753982 38-39 0.041359793926640784 40-41 0.005514639190218771 42-43 0.011827449842179734 44-45 0.010593912128578166 46-47 0.005550919711207053 48-49 0.0010884156296484418 50-51 0.0017051844864492253 52-53 7.618909407539092E-4 54-55 0.0014149403185429743 56-57 0.01197257192613286 58-59 0.009324093893988318 60-61 0.005405797627253927 62-63 0.005550919711207053 64-65 0.07176287051482058 66-67 0.03214454159561731 68-69 0.024815876355984468 70-71 0.01534666037804303 72-73 1.4512208395312556E-4 74-75 4.353662518593767E-4 76-77 6.530493777890651E-4 78-79 5.804883358125023E-4 80-81 6.530493777890651E-4 82-83 6.167688568007836E-4 84-85 8.344519827304719E-4 86-87 0.004535065123535174 88-89 1.8140260494140697E-4 90-91 9.432935456953162E-4 92-93 4.716467728476581E-4 94-95 0.0029024416790625115 96-97 0.0056234807531836154 98-99 0.007110982113703153 100-101 0.008925008163117223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1378150.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.83409177007612 #Duplication Level Percentage of deduplicated Percentage of total 1 83.7792075377363 47.61515169623958 2 11.0056685250945 12.509943498925184 3 2.274896969443311 3.8787510938642753 4 0.9058002784791442 2.059213446097768 5 0.483940088156846 1.3752147690762457 6 0.312397892887192 1.0652910307877448 7 0.21392440515078842 0.8510739491939198 8 0.1553084390955146 0.7061451264177404 9 0.11981446340168572 0.6128591587518449 >10 0.6729832888970627 6.962036420288897 >50 0.045219782696438206 1.764776171860054 >100 0.023225532050296316 2.442954901857043 >500 0.002193517754002938 0.8566061352748042 >1k 0.003870913683534597 5.224116759242061 >5k 9.032131928247394E-4 4.02321083755212 >10k+ 6.451522805890996E-4 8.052655004570754 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 53326 3.869390124442187 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19931 1.4462141276348728 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13231 0.9600551463919021 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12761 0.9259514566629178 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10560 0.766244603272503 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9557 0.6934658781700106 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8682 0.6299749664405181 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8433 0.611907266988354 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8085 0.5866560243805101 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8009 0.5811413851902913 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6835 0.4959547219098066 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5261 0.3817436418386968 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4754 0.3449551935565795 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4288 0.31114174799550126 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4098 0.2973551500199543 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4057 0.2943801472989152 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3774 0.2738453724195479 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3692 0.2678953669774698 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3660 0.26557341363421977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3191 0.23154228494721182 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3164 0.22958313681384462 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2901 0.21049958277400863 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2666 0.1934477379095164 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2612 0.189529441642782 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2540 0.18430504662046948 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2383 0.1729129630301491 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2198 0.159489170264485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1894 0.1374306135036099 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1704 0.12364401552806298 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1677 0.12168486739469579 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1541 0.11181656568588325 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1527 0.11080071109821138 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1521 0.110365344846352 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1519 0.11022022276239887 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1437 0.10427021732032071 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1392 0.10100497043137538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.256104197656278E-5 0.0 14-15 0.0 0.0 0.0 7.256104197656278E-5 0.0 16-17 0.0 0.0 0.0 7.256104197656278E-5 0.0 18-19 0.0 0.0 0.0 1.0884156296484417E-4 0.0 20-21 0.0 3.628052098828139E-5 0.0 1.8140260494140697E-4 0.0 22-23 0.0 7.256104197656278E-5 0.0 4.716467728476581E-4 0.0 24-25 0.0 7.256104197656278E-5 0.0 9.070130247070347E-4 0.0 26-27 0.0 7.256104197656278E-5 0.0 0.0012698182345898487 0.0 28-29 0.0 7.256104197656278E-5 0.0 0.0027210390741211044 0.0 30-31 0.0 7.256104197656278E-5 0.0 0.00573232231614846 0.0 32-33 0.0 7.256104197656278E-5 0.0 0.01142836411130864 0.0 34-35 0.0 1.0884156296484417E-4 0.0 0.016217392881761782 0.0 36-37 0.0 1.4512208395312556E-4 0.0 0.02057105540035555 0.0 38-39 0.0 1.4512208395312556E-4 0.0 0.023763741247324312 0.0 40-41 0.0 1.4512208395312556E-4 0.0 0.029205819395566518 0.0 42-43 0.0 1.4512208395312556E-4 0.0 0.0380219859957189 0.0 44-45 0.0 1.4512208395312556E-4 0.0 0.05427565939846896 0.0 46-47 0.0 2.1768312592968834E-4 0.0 0.08772629974966441 0.0 48-49 0.0 2.902441679062511E-4 0.0 0.2076334216159344 0.0 50-51 0.0 2.902441679062511E-4 0.0 0.26401335123172365 0.0 52-53 0.0 2.902441679062511E-4 0.0 0.3140441896745637 0.0 54-55 0.0 2.902441679062511E-4 0.0 0.38308602111526324 0.0 56-57 0.0 2.902441679062511E-4 0.0 0.42274063055545474 0.0 58-59 0.0 2.902441679062511E-4 0.0 0.48365562529477923 0.0 60-61 0.0 2.902441679062511E-4 0.0 0.6053767732104633 0.0 62-63 0.0 2.902441679062511E-4 0.0 0.6637521314806081 0.0 64-65 0.0 2.902441679062511E-4 0.0 0.7366396981460654 0.0 66-67 0.0 2.902441679062511E-4 0.0 0.9476109276929217 0.0 68-69 0.0 2.902441679062511E-4 0.0 1.151108369916192 0.0 70-71 0.0 2.902441679062511E-4 0.0 1.2897362406124153 0.0 72-73 0.0 3.6280520988281393E-4 0.0 1.4213256902369118 0.0 74-75 0.0 3.6280520988281393E-4 0.0 1.5152922395965605 0.0 76-77 0.0 3.6280520988281393E-4 0.0 1.5672459456517795 0.0 78-79 0.0 3.6280520988281393E-4 0.0 1.659978957297827 0.0 80-81 0.0 3.6280520988281393E-4 0.0 1.8341254580415773 0.0 82-83 0.0 3.6280520988281393E-4 0.0 2.091318071327504 0.0 84-85 0.0 3.6280520988281393E-4 0.0 2.445343395131154 0.0 86-87 0.0 3.6280520988281393E-4 0.0 3.1500199542865435 0.0 88-89 0.0 3.6280520988281393E-4 0.0 3.9221782824801363 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2080 0.0 53.214176 2 TATAACG 310 0.0 50.569305 2 ACCTGGG 2190 0.0 48.370403 3 GTACCTG 2330 0.0 48.348103 1 ATAACGC 360 0.0 43.54421 3 TAACGCA 380 0.0 41.237446 4 GTACACG 185 0.0 41.10885 1 TACACGG 225 0.0 38.003597 2 TACGCAG 820 0.0 36.482796 4 AGTACGC 830 0.0 36.057632 2 GTACCCG 225 0.0 35.913143 1 GTACGCA 850 0.0 35.20794 3 CCTGGGG 1420 0.0 35.11255 4 GAGTACG 910 0.0 34.996166 1 ACGCAGT 915 0.0 32.694965 5 GTATAAC 515 0.0 32.30337 1 CGCAGTG 985 0.0 30.371464 6 TCACGCA 210 1.8189894E-12 29.395813 4 ACAACGC 130 3.036057E-7 29.23248 3 GTAGGCA 925 0.0 28.244896 3 >>END_MODULE