##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003820_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1801853 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67085494765666 31.0 31.0 34.0 30.0 34.0 2 31.868702385821706 31.0 31.0 34.0 30.0 34.0 3 31.926662718878845 31.0 31.0 34.0 30.0 34.0 4 35.69815351196796 37.0 35.0 37.0 33.0 37.0 5 35.554948711132376 37.0 35.0 37.0 33.0 37.0 6 35.6273974625011 37.0 35.0 37.0 33.0 37.0 7 35.57733289008593 37.0 35.0 37.0 33.0 37.0 8 35.57400076476827 37.0 35.0 37.0 33.0 37.0 9 37.170073807352765 39.0 37.0 39.0 33.0 39.0 10-11 37.06786596908849 39.0 37.0 39.0 33.0 39.0 12-13 37.029292622650125 39.0 37.0 39.0 33.0 39.0 14-15 38.14631604242966 40.0 37.0 41.0 33.0 41.0 16-17 38.14225744275476 40.0 37.0 41.0 33.0 41.0 18-19 38.15364821658592 40.0 37.0 41.0 33.0 41.0 20-21 38.09595871583309 40.0 37.0 41.0 33.0 41.0 22-23 38.01342701097148 40.0 37.0 41.0 33.0 41.0 24-25 37.90279700952297 40.0 37.0 41.0 33.0 41.0 26-27 37.68546352005408 39.5 37.0 41.0 32.5 41.0 28-29 37.511520362648895 39.0 36.5 41.0 32.0 41.0 30-31 37.32832978050929 39.0 36.0 40.5 31.5 41.0 32-33 37.072669357600205 39.0 36.0 40.0 31.0 41.0 34-35 37.042766529789056 39.0 36.0 40.0 31.0 41.0 36-37 36.8854090761011 39.0 35.5 40.0 30.5 41.0 38-39 36.64272002211057 39.0 35.0 40.0 30.0 41.0 40-41 36.672264052616946 39.0 35.0 40.0 30.0 41.0 42-43 36.55245405701797 39.0 35.0 40.0 30.0 41.0 44-45 36.337676547420905 39.0 35.0 40.0 30.0 41.0 46-47 36.15886839825446 39.0 35.0 40.0 30.0 41.0 48-49 35.936077748850764 39.0 35.0 40.0 29.0 41.0 50-51 35.70713842916153 39.0 35.0 40.0 28.0 41.0 52-53 35.48122377352648 38.0 35.0 40.0 27.5 41.0 54-55 35.30042295348178 38.0 34.0 40.0 27.0 41.0 56-57 35.039556778494145 38.0 34.0 40.0 26.5 41.0 58-59 34.778059308944734 38.0 34.0 40.0 26.0 41.0 60-61 34.418579096074986 38.0 33.5 40.0 26.0 41.0 62-63 34.14471380295729 37.0 33.0 40.0 25.5 41.0 64-65 33.65423649986985 36.5 32.5 39.5 23.5 41.0 66-67 33.29509760230163 36.0 32.0 39.0 23.0 40.5 68-69 33.00724948150598 36.0 32.0 39.0 22.5 40.0 70-71 32.585700942307724 35.0 31.5 38.5 22.0 40.0 72-73 32.16740821809548 35.0 31.0 37.5 21.5 40.0 74-75 31.771020443954086 35.0 31.0 37.0 20.0 39.0 76-77 30.779730366461635 34.0 29.5 36.0 20.0 39.0 78-79 31.221923209052015 34.0 31.0 36.0 20.0 39.0 80-81 31.03466653495041 34.0 31.0 36.0 20.0 37.5 82-83 30.738013311851745 34.0 31.0 35.5 18.5 37.0 84-85 30.397564618201375 34.0 30.5 35.0 18.0 37.0 86-87 30.06504387427831 34.0 30.0 35.0 13.5 36.0 88-89 29.715765381526687 34.0 30.0 35.0 8.5 36.0 90-91 29.409924117006213 34.0 29.0 35.0 6.5 36.0 92-93 29.16429420158026 34.0 29.0 35.0 2.0 35.0 94-95 28.811781815719705 34.0 29.0 35.0 2.0 35.0 96-97 28.42557050991396 34.0 29.0 35.0 2.0 35.0 98-99 28.009883714154263 34.0 29.0 35.0 2.0 35.0 100-101 27.044923198507313 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.02829827092213577 1101 2 0.041589394998279516 1101 3 -0.0028890702836008586 1101 4 -0.025826243585662212 1101 5 -0.017907460789345464 1101 6 0.04972172744264469 1101 7 -0.0829989537884046 1101 8 -0.14334579087237387 1101 9 -0.0499917408240691 1101 10-11 0.08917000909975314 1101 12-13 0.08173657825747682 1101 14-15 0.09151282363806246 1101 16-17 0.12016725834564568 1101 18-19 -0.009011115632176825 1101 20-21 -0.16391134761595083 1101 22-23 -0.11751386292880284 1101 24-25 -0.14690224721234557 1101 26-27 -0.12032245989914259 1101 28-29 -0.04310289185328031 1101 30-31 0.040104525563876336 1101 32-33 -0.033515339427744095 1101 34-35 -0.012252400141186115 1101 36-37 -0.008977422863814866 1101 38-39 -0.23534604581412566 1101 40-41 -0.009863646775535528 1101 42-43 0.021714213825504203 1101 44-45 -0.028731076799438426 1101 46-47 0.10516394410608143 1101 48-49 0.0658349361901216 1101 50-51 0.1765421092540791 1101 52-53 0.013465644770761287 1101 54-55 0.009184113049172993 1101 56-57 -0.09183902010886413 1101 58-59 -0.027016819472386544 1101 60-61 -0.16619277405713717 1101 62-63 -0.3527720833274657 1101 64-65 -0.28066773308218274 1101 66-67 -0.35123218521315636 1101 68-69 -0.2982314143438458 1101 70-71 -0.2578326632080703 1101 72-73 -0.4008025700269826 1101 74-75 -0.3779199881725184 1101 76-77 -0.28591296285654977 1101 78-79 -0.2885613767064825 1101 80-81 -0.32744350394428423 1101 82-83 -0.2804298334482631 1101 84-85 -0.2811961756964685 1101 86-87 -0.5505360476861831 1101 88-89 -0.4765070788145742 1101 90-91 -0.6423561471083872 1101 92-93 -0.508371257546667 1101 94-95 -0.4954393836068185 1101 96-97 -0.6010398467069358 1101 98-99 -0.7434956791220699 1101 100-101 -0.4048310827403654 1102 1 0.01855311270774962 1102 2 0.05979277780652836 1102 3 0.15534309701201465 1102 4 -0.012675580888171112 1102 5 -0.0023782822771778456 1102 6 -0.06056696974381026 1102 7 -0.05207300618253896 1102 8 -0.06339438241086981 1102 9 -0.006603193539831409 1102 10-11 0.04607753859433927 1102 12-13 -0.09953793423026269 1102 14-15 -0.03808435079931627 1102 16-17 -0.015881865936897555 1102 18-19 -0.07130130155092473 1102 20-21 -0.1365130820240097 1102 22-23 -0.12622259734909846 1102 24-25 0.05388353085121622 1102 26-27 -0.042960898120348645 1102 28-29 -0.02046078422151254 1102 30-31 0.11715962360800347 1102 32-33 0.0960178630105375 1102 34-35 -0.003985062807224438 1102 36-37 -0.15009644941772393 1102 38-39 -0.2374499382821469 1102 40-41 -0.27708449151267445 1102 42-43 -0.08287320949303734 1102 44-45 0.08049766551882698 1102 46-47 -0.1345652495108567 1102 48-49 -0.09442045478557048 1102 50-51 0.05546155909100747 1102 52-53 -0.15631905013716363 1102 54-55 -0.10667956427101188 1102 56-57 -0.18136749308462186 1102 58-59 -0.14844110992195425 1102 60-61 -0.4139922440700019 1102 62-63 -0.6506126060845077 1102 64-65 -0.7764848962550417 1102 66-67 -0.4200314006970629 1102 68-69 -0.43731644196441266 1102 70-71 -0.4506576647467 1102 72-73 -0.3042776571560708 1102 74-75 -0.3887698916126574 1102 76-77 -0.3228562584260146 1102 78-79 -0.18369670903373603 1102 80-81 -0.12862920893027407 1102 82-83 -0.23883094894907586 1102 84-85 -0.3478407288253571 1102 86-87 -0.36989145067968465 1102 88-89 -0.3789547309839634 1102 90-91 -0.21189196278826472 1102 92-93 -0.2688257286069593 1102 94-95 -0.12995822777743982 1102 96-97 -0.058174724337913375 1102 98-99 -0.2751722056308452 1102 100-101 -0.24203991549698145 1103 1 -0.15756024761613574 1103 2 0.007853833149486178 1103 3 -0.03325308610471822 1103 4 -0.02304907345344276 1103 5 0.036227452291065276 1103 6 -0.029029925517505717 1103 7 0.074744388551224 1103 8 0.0269882302500406 1103 9 0.031638969648255966 1103 10-11 -0.07140469878697076 1103 12-13 -0.09557130885051635 1103 14-15 -0.050705207472084624 1103 16-17 -0.02657579429484258 1103 18-19 -0.20022032372877874 1103 20-21 -0.20132461031787585 1103 22-23 -0.12611999627316806 1103 24-25 -0.15260252094846294 1103 26-27 -0.14687791757449276 1103 28-29 -0.1724779891586934 1103 30-31 -0.2568678229500847 1103 32-33 -0.19755185334216918 1103 34-35 -0.30205351282231874 1103 36-37 -0.36532331179671473 1103 38-39 -0.20137379780802434 1103 40-41 -0.23353048883827654 1103 42-43 0.0068996508277692215 1103 44-45 -0.024300552365190242 1103 46-47 -0.09614561419143541 1103 48-49 -0.2571975239072728 1103 50-51 -0.1817746205748776 1103 52-53 -0.3046694220275512 1103 54-55 -0.272865577248659 1103 56-57 -0.3149288288089025 1103 58-59 -0.2542228192004288 1103 60-61 -0.18117622967503166 1103 62-63 -0.38911296657477124 1103 64-65 -0.32141677283796355 1103 66-67 -0.28705732518513827 1103 68-69 -0.3862845770897252 1103 70-71 -0.372170306554942 1103 72-73 -0.21315557728128454 1103 74-75 -0.22819520234294544 1103 76-77 -0.34698538172601445 1103 78-79 -0.12967185061089737 1103 80-81 -0.16051719197182734 1103 82-83 -0.08689909085283176 1103 84-85 -0.2861833363099997 1103 86-87 -0.087341336370379 1103 88-89 -0.17863922497270934 1103 90-91 -0.03689088439857713 1103 92-93 -0.11967149423282564 1103 94-95 -0.034900641768157215 1103 96-97 -0.09555201899711463 1103 98-99 -0.05072850065361223 1103 100-101 -0.026290496286854648 1104 1 -0.09117606682517376 1104 2 -0.056609701664640966 1104 3 -0.08951421140461946 1104 4 -0.09937701504723151 1104 5 -0.20122965887163957 1104 6 -0.18617978491951703 1104 7 -0.23654085768874467 1104 8 -0.2607513667883694 1104 9 -0.1792722085371281 1104 10-11 -0.20338493767456356 1104 12-13 -0.16707054781550568 1104 14-15 -0.22825338485773017 1104 16-17 -0.17796242587651534 1104 18-19 -0.2923455462347775 1104 20-21 -0.3054451634226396 1104 22-23 -0.314229146751714 1104 24-25 -0.10459733559129347 1104 26-27 -0.28930497430385316 1104 28-29 -0.2938850009998788 1104 30-31 -0.4011361974309082 1104 32-33 -0.2986398754250885 1104 34-35 -0.22564688794808063 1104 36-37 -0.23180100960131966 1104 38-39 -0.08419182014891646 1104 40-41 0.09680901016851351 1104 42-43 0.07640536503977557 1104 44-45 0.13533556970998717 1104 46-47 0.07113955465514721 1104 48-49 -0.0011743832834767431 1104 50-51 0.038681097506795936 1104 52-53 0.10325630611526293 1104 54-55 -0.0018500469749511694 1104 56-57 -0.07920081498127729 1104 58-59 -0.10629696206862604 1104 60-61 -8.089980366179361E-4 1104 62-63 -0.2318025158872672 1104 64-65 -0.24221108700353966 1104 66-67 -0.24247034791299882 1104 68-69 -0.3357254098426097 1104 70-71 -0.37148537504809553 1104 72-73 -0.24463920857917287 1104 74-75 -0.20139903073599896 1104 76-77 -0.37301084415152275 1104 78-79 -0.2950699106165722 1104 80-81 -0.16841454253808763 1104 82-83 -0.2874692221636188 1104 84-85 -0.13923005612012673 1104 86-87 -0.059931822142893054 1104 88-89 -0.3577339802806101 1104 90-91 -0.41775020901225446 1104 92-93 -0.23165844054760854 1104 94-95 -0.10240251210842999 1104 96-97 -0.23899808870276118 1104 98-99 -0.22310292987735636 1104 100-101 -0.05561162726227309 1105 1 -0.1271548422107287 1105 2 -0.06885893637150176 1105 3 -0.051204109535195386 1105 4 -0.06868076189335426 1105 5 -0.16102268434263323 1105 6 -0.17462767590977535 1105 7 -0.1019992337403437 1105 8 -0.028884815006563258 1105 9 -0.10856439418524388 1105 10-11 -0.027664725937405876 1105 12-13 -0.12964026038522292 1105 14-15 0.006220798768993063 1105 16-17 0.053661904264771465 1105 18-19 -0.032972523090563755 1105 20-21 -0.027132952907038543 1105 22-23 -0.1132219955326974 1105 24-25 -0.05957546747620768 1105 26-27 -0.033956556879161326 1105 28-29 -0.0971919399000356 1105 30-31 -0.09350108047375727 1105 32-33 -0.07209038404508306 1105 34-35 -0.10072596217970187 1105 36-37 -0.05687472132757421 1105 38-39 0.050546126029708205 1105 40-41 -0.01001164220298989 1105 42-43 0.20792308130324955 1105 44-45 0.32430071524292714 1105 46-47 0.15069550285516442 1105 48-49 0.11299517368268397 1105 50-51 0.1590997910187113 1105 52-53 0.007832340987910413 1105 54-55 0.0482643834360843 1105 56-57 0.07465219056663841 1105 58-59 0.0905542474944454 1105 60-61 0.34929352579472095 1105 62-63 0.1980377359868868 1105 64-65 0.09199933407128924 1105 66-67 0.06849815691143846 1105 68-69 -0.05836436235443898 1105 70-71 0.01035181928910589 1105 72-73 -0.00479888399308237 1105 74-75 0.06382230440059189 1105 76-77 -0.046074343486207425 1105 78-79 0.08809427657303814 1105 80-81 0.05340049283627124 1105 82-83 0.21613638101825572 1105 84-85 0.08827696058180479 1105 86-87 0.1533622831003676 1105 88-89 0.20170711941472774 1105 90-91 0.22817628649050903 1105 92-93 0.36093930247386297 1105 94-95 0.3434475449704415 1105 96-97 0.35239627175939603 1105 98-99 0.5539240971496717 1105 100-101 0.5563933862257926 1106 1 -0.14082158670900924 1106 2 -0.058390237187900595 1106 3 -0.0967105848472869 1106 4 -0.00808364055109223 1106 5 -0.12480492539269505 1106 6 -0.14636229235281917 1106 7 -0.1081816349011433 1106 8 -0.07180512655538251 1106 9 0.08486835513303248 1106 10-11 -0.0021773054715836793 1106 12-13 -0.028601625329308433 1106 14-15 -0.01835661727710658 1106 16-17 -0.027420740397786858 1106 18-19 -0.10242107917519405 1106 20-21 -0.11857536724362205 1106 22-23 -0.07064202355208238 1106 24-25 -0.146863445827897 1106 26-27 0.0728428335325404 1106 28-29 -0.20536259126723166 1106 30-31 -0.1519852820465104 1106 32-33 -0.054686679816320805 1106 34-35 -0.08520168288652741 1106 36-37 0.029804502635663255 1106 38-39 0.030425959581243944 1106 40-41 0.11956600316839427 1106 42-43 0.2338368383185241 1106 44-45 0.3004972720280321 1106 46-47 0.11263792970039788 1106 48-49 0.04239738521232539 1106 50-51 -0.04420176338975779 1106 52-53 -0.002050015141747963 1106 54-55 0.20625855857389297 1106 56-57 0.08931858482266364 1106 58-59 0.3357842816013701 1106 60-61 0.07879497262877777 1106 62-63 -0.021041919107886997 1106 64-65 -0.07256448923983072 1106 66-67 -0.1995554760179985 1106 68-69 -0.19518868667876177 1106 70-71 -0.020603445602446868 1106 72-73 0.08938813702978621 1106 74-75 0.13948505446938952 1106 76-77 0.07457368806895204 1106 78-79 0.19017686286188962 1106 80-81 0.1552626141368343 1106 82-83 0.1732806975038237 1106 84-85 0.09564403530849219 1106 86-87 0.3274160035903684 1106 88-89 0.1659000611994621 1106 90-91 0.2555327080154015 1106 92-93 0.16648292848185164 1106 94-95 0.2909763565651282 1106 96-97 0.3636186172149998 1106 98-99 0.4216054934487765 1106 100-101 0.42586612570393356 1107 1 -0.08246253684003335 1107 2 -0.11717618757819892 1107 3 -0.044072785820958416 1107 4 -0.07873236051278809 1107 5 -0.06731384628706394 1107 6 0.004481620033686795 1107 7 0.023869007077642834 1107 8 0.05538439677521012 1107 9 -0.002590778789517856 1107 10-11 -0.06499288324042851 1107 12-13 0.008878642580917528 1107 14-15 0.05482396043748139 1107 16-17 -0.024076234088951765 1107 18-19 -0.005487527326671682 1107 20-21 -0.10628434047551139 1107 22-23 -0.06478631110455524 1107 24-25 0.016553380323500733 1107 26-27 0.020017059594451325 1107 28-29 -0.050644315462960776 1107 30-31 0.07009117294261813 1107 32-33 0.11887732451057076 1107 34-35 -0.046691849100362504 1107 36-37 0.14892678189854536 1107 38-39 0.18311103120768024 1107 40-41 0.22965071397947412 1107 42-43 0.1419260002307965 1107 44-45 0.4490238321142854 1107 46-47 0.44428943778075336 1107 48-49 0.4215240902518076 1107 50-51 0.3842874248118662 1107 52-53 0.2966136294551305 1107 54-55 0.4031209000388358 1107 56-57 0.5245914514355476 1107 58-59 0.4032659182463263 1107 60-61 0.26809791884534206 1107 62-63 0.3656823188324765 1107 64-65 0.43806174608847925 1107 66-67 0.5594216450082925 1107 68-69 0.5185187393402231 1107 70-71 0.4601729164091992 1107 72-73 0.31781051650575876 1107 74-75 0.4853397479029553 1107 76-77 0.4070630185707529 1107 78-79 0.37776105210664923 1107 80-81 0.5275712716552903 1107 82-83 0.6271012762796353 1107 84-85 0.5535488099616934 1107 86-87 0.4308802892615873 1107 88-89 0.3225577798382524 1107 90-91 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-0.4728291072508206 2316 76-77 -0.17493984735171253 2316 78-79 -0.21536971439095254 2316 80-81 -0.24280742837165192 2316 82-83 -0.250097460215585 2316 84-85 -0.3802089454041031 2316 86-87 -0.4857463560082742 2316 88-89 -0.22856418585657678 2316 90-91 -0.22653229321806734 2316 92-93 -0.1291700626355059 2316 94-95 -0.22775808873519665 2316 96-97 -0.45141890705578547 2316 98-99 -0.410165866940293 2316 100-101 -0.9706008282684202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 17.0 5 38.0 6 87.0 7 233.0 8 522.0 9 983.0 10 1768.0 11 3043.0 12 4644.0 13 6543.0 14 7659.0 15 9690.0 16 12130.0 17 15178.0 18 13720.0 19 9988.0 20 9293.0 21 9447.0 22 10215.0 23 11347.0 24 13071.0 25 16162.0 26 20063.0 27 25012.0 28 31623.0 29 39536.0 30 49621.0 31 61387.0 32 76595.0 33 97300.0 34 128687.0 35 174772.0 36 251239.0 37 344668.0 38 297430.0 39 48121.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19828025626247 20.495223119148278 14.425980279472238 20.88051634511701 2 15.53016995697102 22.862356664885127 38.84895679841759 22.758516579726265 3 21.216124992227954 27.106445911016763 29.89849710879973 21.778931987955552 4 13.012215757889239 17.587117262063 39.36991530385664 30.03075167619112 5 12.275252198708774 39.19370781079255 34.59288854307205 13.938151447426621 6 28.055396305913966 38.85100504869154 18.054358485403636 15.039240159990856 7 27.100656934833196 32.91056484629989 22.62548609681256 17.363292122054354 8 24.554111794913347 33.69092817227599 20.17234480282243 21.58261522998824 9 24.230722484020617 19.859944179686135 22.852419148509895 33.056914187783356 10-11 24.54822896207404 26.308194952640417 28.464669426418247 20.678906658867287 12-13 27.304114153596327 24.014389631118632 27.88743032866721 20.794065886617833 14-15 22.440355149647694 26.58100200240644 28.27752531867566 22.70111752927021 16-17 20.459882132449206 29.500908231692595 28.79519028466806 21.244019351190136 18-19 20.105163961766024 30.57349850404001 29.69176730843193 19.629570225762034 20-21 21.670646775834054 27.860412645507566 28.772151354231568 21.696789224426816 22-23 20.68906287027854 28.09346822410041 28.983829424486903 22.233639481134144 24-25 22.351684626881326 28.028202078637936 29.10972759709033 20.51038569739041 26-27 22.11097306719008 26.69340531474338 31.01312243601609 20.182499182050453 28-29 20.61941015719206 29.205153388603055 30.113071382641298 20.062365071563587 30-31 21.34149641211763 28.157737501384116 29.265031516251828 21.23573457024643 32-33 21.43221074142793 28.291530526104296 29.537900034022424 20.738358698445346 34-35 22.51958295187729 26.965461797460648 29.862076410351328 20.652878840310727 36-37 22.499181934221127 26.858730101018285 30.711618698915988 19.930469265844593 38-39 21.755629619635975 27.955137376654715 30.531362584758675 19.75787041895063 40-41 20.924375297969398 28.54786852327616 29.89170702955246 20.63604914920198 42-43 20.54631924561332 29.038941807223335 28.580396414337656 21.834342532825694 44-45 21.548324538778466 27.9256117752124 28.3719722059874 22.154091480021734 46-47 22.385425483204063 28.515047661333963 28.28305421043138 20.816472645030597 48-49 22.32781621890119 28.223807350078463 28.698883210980025 20.74949322004032 50-51 20.94966912373776 28.758970510984394 29.745591438370322 20.545768926907524 52-53 21.277885214935825 29.518430860650724 28.227353491189643 20.976330433223804 54-55 20.485179091584417 28.721835223588027 28.68834148847047 22.104644196357093 56-57 22.17881079580934 27.97554985082911 28.374023451051134 21.471615902310415 58-59 23.20418652887702 27.49847573117032 28.07879408342253 21.218543656530127 60-61 21.673172731084613 28.12845404864799 29.877945732013416 20.32042748825398 62-63 21.374739562231447 28.033843312202304 29.185236328196545 21.406180797369704 64-65 20.80286325905369 29.76901381229814 28.689543600749857 20.73857932789831 66-67 21.38139760309698 27.885191376389944 28.23750403194242 22.495906988570656 68-69 20.71585513029325 29.07797166422967 28.119244053784893 22.086929151692186 70-71 22.181591006291455 28.594662268282033 27.674798183029754 21.548948542396758 72-73 21.5581110176577 27.625241522802835 29.194574349619295 21.622073109920173 74-75 20.783318667822147 29.736911551566685 28.15714324166903 21.322626538942135 76-77 21.541876429443953 27.772739709529777 28.06865992872829 22.616723932297983 78-79 21.468633695149066 28.330099598299068 28.585005977229915 21.616260729321947 80-81 22.010055327053422 28.101664362529004 28.23566694101722 21.65261336940035 82-83 21.223447207985195 27.601722688259468 29.221821087946452 21.953009015808885 84-85 20.810763711600195 28.45795279471116 28.7380843497063 21.99319914398235 86-87 21.169554745661916 28.757252342242563 27.8662274999438 22.206965412151717 88-89 20.970369611890447 28.509789524596812 27.54980368782187 22.970037175690866 90-91 21.187882474353252 27.32919995793181 28.240908650701655 23.24200891701328 92-93 21.434868547202605 28.651806523814905 28.06893158649373 21.844393342488765 94-95 20.980701327907596 28.556978591194714 28.11536023780901 22.346959843088676 96-97 19.956586013336675 28.57697088371218 28.80991450685968 22.656528596091466 98-99 20.890334830924967 29.236061754910235 27.158240088850878 22.715363325313927 100-101 20.19775865384909 28.333343971785713 27.609614681730342 23.85928269263485 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 348.0 1 251.0 2 430.0 3 665.5 4 484.0 5 364.0 6 493.5 7 821.5 8 1205.0 9 1419.5 10 1397.0 11 1367.0 12 1479.5 13 1614.0 14 1745.5 15 1919.0 16 2140.5 17 2350.0 18 2525.5 19 2726.0 20 2929.0 21 3227.0 22 3790.0 23 4548.5 24 5506.5 25 6658.0 26 8344.0 27 10639.5 28 13682.0 29 17651.5 30 21886.0 31 26006.0 32 29970.0 33 34137.0 34 38338.0 35 43027.5 36 47844.0 37 52596.0 38 57566.5 39 60955.0 40 63351.0 41 65394.0 42 67671.0 43 70747.5 44 71562.5 45 72526.0 46 76309.0 47 93246.0 48 139850.5 49 136614.5 50 89724.0 51 68642.0 52 52970.0 53 42497.5 54 35701.5 55 30490.5 56 25178.0 57 20023.0 58 16024.5 59 12990.0 60 10416.5 61 7969.5 62 5873.0 63 4110.0 64 2697.0 65 1784.5 66 1104.0 67 627.5 68 378.0 69 209.5 70 119.0 71 66.0 72 39.0 73 25.5 74 13.5 75 10.0 76 7.5 77 5.5 78 4.0 79 2.0 80 1.5 81 1.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04922710121191906 2 0.0024419306125416447 3 0.029136672081462804 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011654668832585123 16-17 0.0 18-19 0.0 20-21 0.010294957468783525 22-23 0.0 24-25 0.0 26-27 0.0050781057056263745 28-29 0.011293929083005107 30-31 0.009073992162512702 32-33 0.005549842301231011 34-35 0.005244600974663305 36-37 0.019285701996777763 38-39 0.03940388033874017 40-41 0.004106883302910948 42-43 0.009850970084685043 44-45 0.009434731912092717 46-47 0.004328876994960188 48-49 0.001970194016937009 50-51 0.00258067667007242 52-53 0.0013597113638015976 54-55 0.0013042129407892873 56-57 0.012653640446806703 58-59 0.009406982700586562 60-61 0.004023635668392483 62-63 0.004384375417972499 64-65 0.06923428270785686 66-67 0.033965034883533786 68-69 0.025251782470601097 70-71 0.015678304500977604 72-73 2.497429035553955E-4 74-75 3.607397495800157E-4 76-77 8.879747681969617E-4 78-79 8.324763451846517E-4 80-81 0.0012209653062708224 82-83 9.98971614221582E-4 84-85 0.0011654668832585123 86-87 0.005966080473823336 88-89 1.9424448054308535E-4 90-91 9.157239797031167E-4 92-93 4.7173659560463587E-4 94-95 0.003940388033874018 96-97 0.007187045780094158 98-99 0.010128462199746594 100-101 0.011793414890115896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1801853.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.84798449431759 #Duplication Level Percentage of deduplicated Percentage of total 1 80.73309066105388 45.087803954168734 2 11.892649434861376 13.28361002468986 3 3.0988634432107665 5.191958325793275 4 1.3017753483145085 2.9080611787101422 5 0.694843281091255 1.9402798394182588 6 0.44750489599267707 1.499534789551814 7 0.32649715443564226 1.2763945612852396 8 0.21758395191479163 0.9721300138199702 9 0.16446848899602476 0.8266710260928453 >10 1.0086233925634913 10.504169111764087 >50 0.07006305266912456 2.6653837111536616 >100 0.03706327143738675 3.7410598594777884 >500 0.002689826191393641 1.0550547653388818 >1k 0.0033871885373105114 4.368548756255778 >5k 6.973623459168699E-4 2.477706790319908 >10k+ 1.992463845476771E-4 2.2016332921597264 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28328 1.5721593270927208 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11243 0.6239687699274025 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8092 0.4490932390156134 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7215 0.40042112203381747 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7009 0.38898844689328155 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6230 0.3457551753666919 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5882 0.32644172415840805 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5104 0.28326395105483076 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5001 0.27754761348456286 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4987 0.2767706355623905 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4952 0.27482819075695963 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4585 0.25446026951144185 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4568 0.25351679632023255 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4216 0.23398135141989942 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3930 0.21810880243837868 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3884 0.21555587497981243 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3265 0.18120235113519248 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2660 0.14762580521274488 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2633 0.1461273477914125 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2477 0.13746959380149215 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2382 0.13219724361532267 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2288 0.1269803918521655 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2219 0.12315100066431611 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2197 0.12193003535804531 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2120 0.11765665678609742 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2059 0.1142712529823465 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2042 0.11332777979113724 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1864 0.10344906049494604 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.3874605753077526E-4 0.0 12-13 0.0 0.0 0.0 1.664952690369303E-4 0.0 14-15 0.0 0.0 0.0 2.2199369204924042E-4 0.0 16-17 0.0 0.0 0.0 3.052413265677056E-4 0.0 18-19 0.0 5.5498423012310106E-5 0.0 6.382318646415662E-4 0.0 20-21 0.0 5.5498423012310106E-5 0.0 0.001054470037233892 0.0 22-23 0.0 5.5498423012310106E-5 0.0 0.0016372034788631482 0.0 24-25 5.5498423012310106E-5 5.5498423012310106E-5 0.0 0.0022476861319985593 0.0 26-27 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.003579648284294002 0.0 28-29 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.0059105820508110266 0.0 30-31 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.012265151485720533 0.0 32-33 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.0252517824706011 0.0 34-35 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.04004211220338175 0.0 36-37 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.05455494982110083 0.0 38-39 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.08052821179086196 0.0 40-41 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.14584985567635095 0.0 42-43 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.20007181495937792 0.0 44-45 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.2601488578702036 0.0 46-47 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.3871292497223692 0.0 48-49 5.5498423012310106E-5 1.1099684602462021E-4 0.0 0.4609421523287416 0.0 50-51 5.5498423012310106E-5 1.3874605753077526E-4 0.0 0.566416905263637 0.0 52-53 5.5498423012310106E-5 1.664952690369303E-4 0.0 0.6908998680802485 0.0 54-55 5.5498423012310106E-5 1.664952690369303E-4 0.0 0.826288271018779 0.0 56-57 5.5498423012310106E-5 1.664952690369303E-4 0.0 1.108691996516919 0.0 58-59 5.5498423012310106E-5 1.664952690369303E-4 0.0 1.3853238860217787 0.0 60-61 5.5498423012310106E-5 1.664952690369303E-4 0.0 1.6076228193975868 0.0 62-63 5.5498423012310106E-5 1.664952690369303E-4 0.0 1.851593886959702 0.0 64-65 5.5498423012310106E-5 1.664952690369303E-4 0.0 2.1039729656081825 0.0 66-67 5.5498423012310106E-5 1.664952690369303E-4 0.0 2.4223396692182995 0.0 68-69 5.5498423012310106E-5 1.664952690369303E-4 0.0 2.779888259475107 0.0 70-71 5.5498423012310106E-5 1.664952690369303E-4 0.0 3.0137031156259697 0.0 72-73 5.5498423012310106E-5 2.2199369204924042E-4 0.0 3.273102744785507 0.0 74-75 5.5498423012310106E-5 2.2199369204924042E-4 0.0 3.6710541869952764 0.0 76-77 5.5498423012310106E-5 2.2199369204924042E-4 0.0 4.091343744467501 0.0 78-79 5.5498423012310106E-5 2.2199369204924042E-4 0.0 4.5146857152054025 0.0 80-81 5.5498423012310106E-5 2.2199369204924042E-4 0.0 4.926733756860299 0.0 82-83 5.5498423012310106E-5 2.2199369204924042E-4 0.0 5.417006825751047 0.0 84-85 5.5498423012310106E-5 2.2199369204924042E-4 0.0 5.986448395068854 0.0 86-87 5.5498423012310106E-5 2.2199369204924042E-4 0.0 6.630452095703701 0.0 88-89 5.5498423012310106E-5 3.329905380738606E-4 0.0 7.265132061272479 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2290 0.0 50.192913 2 ACCTGGG 2470 0.0 44.804485 3 TATAACG 310 0.0 42.900154 2 GTACCTG 2910 0.0 40.49506 1 TAACGCA 325 0.0 39.44886 4 ATAACGC 415 0.0 35.47939 3 GTACCCG 260 0.0 29.24085 1 TACGCAG 490 0.0 29.072289 4 AGTACGC 480 0.0 28.695862 2 TACCCGG 260 0.0 27.401884 2 CCTGGGG 2060 0.0 26.73898 4 GTACGCA 510 0.0 26.076563 3 TCACGCA 315 0.0 25.626682 4 GAGTACG 575 0.0 24.791157 1 TATCACG 315 0.0 24.125256 2 GTATAAC 605 0.0 23.561842 1 GTACCGG 135 3.7882675E-4 21.118391 1 ACGCAGT 630 0.0 21.104326 5 CTGGGGG 1160 0.0 20.876911 5 TGCGACG 110 1.348737E-6 19.42827 22-23 >>END_MODULE