FastQCFastQC Report
Wed 25 May 2016
SRR1003819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5185388
Sequences flagged as poor quality0
Sequence length121
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT413460.7973559548485089No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT275310.5309342328867194No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC179300.34577933223126217No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT175580.33860532712306196No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT130850.2523437011849451No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT113990.21982925867842484No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC102340.19736228031537853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92970.17929227282510007No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC88550.17076832051912025No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88150.16999692212038905No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG82300.1587152205389452No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC81200.1565938749424344No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG70710.13636395193570858No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA69930.13485972505818272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68190.131504142023702No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN64010.1234430287569609No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA62670.12085884412121137No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA61520.11864107372485916No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC54060.1042544935885222No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA53090.10238385247159905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG191700.023.2207551
GAGTACT127200.022.9494112-13
TATAACG7750.022.2657532
TACATGG188650.021.831022
ACATGGG186300.021.0042933
GTACTTT140600.020.86437414-15
TCTCGGG23350.020.68169114-115
AGTACTT139700.020.19668212-13
CCGGTCG6750.018.715679
GTATAGG25200.018.5338481
TAACGCA8300.017.9981044
GTCGCCG18700.017.831177114-115
CATGGGG91350.017.7995664
CTAACGC4502.9540388E-916.5998673
GGCGTCG7650.016.5149528
GTACTAG16500.016.424611
GAGTACG13450.016.29081
TACTTTT183650.015.83268214-15
TATACTG34500.015.8213845
GCGTGCG9450.015.7989419
Produced by FastQC (version 0.11.5)