FastQCFastQC Report
Wed 25 May 2016
SRR1003819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5185388
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT486580.938367582136573No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT238100.4591748968447491No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT182210.35139125558203166No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC175070.3376217941646797No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT133140.25675995701768123No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT122300.23585506041206558No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC122090.2354500762527317No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC113000.21792004764156508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107420.2071590399792648No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG99570.19202034640416493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89340.17229183235661438No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC86840.16747059236454437No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA84340.16264935237247433No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA84310.16259149749256951No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG82380.15886950021869145No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA69570.13416546649932462No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT58340.11250845645494609No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA57520.11092708973754713No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC53680.10352166510972757No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA52630.10149674431305816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52300.10086034063410491No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG6350.022.4497472
TAACGCA6600.021.587514
GAGTACT147300.019.55535912-13
GTACATG201750.019.5344831
GGCGTCG6350.019.4455668
AGTACTT152650.019.13445312-13
TACATGG199600.018.926512
GTACTTT155400.018.35454814-15
ACATGGG200200.017.8476093
CCGATCG3208.303687E-917.810219
CATGGGG93450.017.7874584
GTATAAC16500.017.5754641
TAGACCG1901.8695224E-417.4972445
TACCTGG31600.017.14282
ACGTGCG3401.7562343E-816.762559
GTACCGG4451.1277734E-1016.0247841
GAGTACG8850.015.5781421
TACTTTT190650.015.40936114-15
ATAACGC10200.015.372343
GCGTAAT2801.3287936E-515.2807761
Produced by FastQC (version 0.11.5)