FastQCFastQC Report
Wed 25 May 2016
SRR1003818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905895
Sequences flagged as poor quality0
Sequence length121
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114420.6003478680619867No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT95350.5002898900516555No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56910.2985998704020945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51460.2700043811437671No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45020.23621448191007374No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44160.2317021661738973No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39750.20856343082908554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37060.19444932695662667No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33010.17319946796649344No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28830.15126751473717073No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT26720.14019660054724944No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24900.130647281198597No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23850.12513805849745133No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.12204240002728377No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22060.11574614551168874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21950.11516898884775918No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19570.10268141739182904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3550.059.9626352
TACCTGG22300.056.7577482
TAACGCA4800.051.4733354
ACCTGGG27400.044.666733
GTACACG3150.040.2598531
GTACCTG32700.039.6638451
ATAACGC6600.036.564573
TACACGG3350.036.064662
GTATAAC7250.033.3942261
ACACGGG4150.027.6908933
TATCACG3650.023.6431712
CCTGGGG19750.023.5653024
GTACATG79550.023.333281
TACATGG76850.022.1592562
AGTACTT67100.021.36614212-13
GAGTACT65250.021.04725612-13
TCTCGGG10450.020.903446114-115
ACATGGG76750.020.662643
CCTGGGA20850.019.566244
GAGTACC8300.019.4464361
Produced by FastQC (version 0.11.5)