FastQCFastQC Report
Wed 25 May 2016
SRR1003818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905895
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT141590.7429055640525842No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT81460.427410744033643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61520.3227879814995055No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56800.298022713738165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53380.2800783883687191No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA51940.2725228829500051No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA49320.258776060590956No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38180.20032583117118202No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32320.16957912162002628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.16265324165287176No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30120.1580359883414354No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28420.1491162944443424No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27450.14402682204423645No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26160.13725834843997178No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25880.13578922238633293No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23690.12429855789537199No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA21410.11233567431574142No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19600.10283882375471891No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19600.10283882375471891No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4050.055.1409572
TACCTGG24100.046.9236072
TAACGCA5450.042.6986854
GTACACG2450.040.7443241
ATAACGC5800.038.5025863
GTATAAC6500.038.028031
ACCTGGG28950.037.9125943
GTACCTG31150.036.776711
CCTGGGG16600.030.3258324
TACACGG3000.026.925212
TATCACG3200.026.7272322
TCACGCA3100.026.0436694
ACACGGG3800.023.7569163
TACGCAG2457.54153E-921.3226684
CATGGGT13500.021.1072864
GTACATG86550.019.8268621
TACATGG83350.019.2112562
CACGCAG4250.018.996565
GAACCGC1759.954973E-518.9965576
TAAGACG1507.7330245E-418.9965574
Produced by FastQC (version 0.11.5)