##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003817_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 631558 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.52190139306287 31.0 31.0 34.0 27.0 34.0 2 30.415198604087035 31.0 31.0 34.0 26.0 34.0 3 30.5068782281279 31.0 31.0 34.0 26.0 34.0 4 34.04099860978722 37.0 35.0 37.0 30.0 37.0 5 34.029800905063354 37.0 35.0 37.0 30.0 37.0 6 34.11534490893948 37.0 35.0 37.0 30.0 37.0 7 34.09185696325595 37.0 35.0 37.0 30.0 37.0 8 34.06100627337474 37.0 35.0 37.0 30.0 37.0 9 35.48373071040189 39.0 35.0 39.0 30.0 39.0 10-11 35.341330487461164 38.0 35.0 39.0 30.0 39.0 12-13 35.198517000813865 38.0 35.0 39.0 29.0 39.0 14-15 36.12989305811976 39.0 36.0 41.0 27.0 41.0 16-17 36.137219384442915 39.0 36.0 41.0 28.5 41.0 18-19 36.06409704255191 39.0 36.0 41.0 27.5 41.0 20-21 35.99650071727379 39.0 35.5 41.0 27.0 41.0 22-23 35.87865801715757 39.0 35.0 40.5 27.0 41.0 24-25 35.62009269140759 39.0 35.0 40.5 27.0 41.0 26-27 35.486172291380996 38.5 34.5 40.0 27.0 41.0 28-29 35.28687705642237 38.0 34.0 40.0 26.0 41.0 30-31 34.98779209510448 38.0 34.0 40.0 25.0 41.0 32-33 34.691378305713805 38.0 34.0 40.0 24.5 41.0 34-35 34.62217405210606 38.0 34.0 40.0 24.0 41.0 36-37 34.42241409340076 38.0 33.5 40.0 24.0 41.0 38-39 34.35716038748619 38.0 33.5 40.0 24.0 41.0 40-41 34.435035262002856 38.0 34.0 40.0 24.0 41.0 42-43 34.27692151789701 38.0 33.0 40.0 22.5 41.0 44-45 34.021355124944975 38.0 33.0 40.0 20.0 41.0 46-47 33.70905205855994 38.0 33.0 40.0 16.5 41.0 48-49 33.39942095579504 38.0 33.0 40.0 14.5 41.0 50-51 32.53741540761102 37.5 32.0 39.5 9.5 40.5 52-53 32.6267706212256 37.5 31.5 39.5 9.0 40.5 54-55 32.92730754736699 38.0 32.0 40.0 8.5 41.0 56-57 32.744130388657894 38.0 32.0 40.0 7.5 41.0 58-59 32.478677334464926 37.0 31.5 40.0 6.5 41.0 60-61 32.15560328584232 37.0 31.0 40.0 2.0 41.0 62-63 31.851225857324266 36.5 31.0 40.0 2.0 41.0 64-65 31.545918189619954 36.0 30.5 39.0 2.0 41.0 66-67 31.162958904803673 36.0 30.0 39.0 2.0 41.0 68-69 30.77826264571108 35.0 29.5 39.0 2.0 40.0 70-71 30.326294655439405 35.0 29.0 38.0 2.0 40.0 72-73 29.934033770453386 35.0 29.0 37.0 2.0 40.0 74-75 29.560302458364866 34.5 29.0 37.0 2.0 39.0 76-77 29.102413396710993 34.0 28.5 36.5 2.0 39.0 78-79 28.571147859737348 34.0 27.0 36.0 2.0 38.5 80-81 28.15194091437366 34.0 26.0 35.5 2.0 37.5 82-83 27.76474053056093 34.0 26.0 35.0 2.0 37.0 84-85 27.467431336472657 34.0 26.0 35.0 2.0 36.5 86-87 27.193960808033466 34.0 25.5 35.0 2.0 36.0 88-89 26.82253332235519 33.0 25.0 35.0 2.0 36.0 90-91 26.447207540716768 33.0 24.5 35.0 2.0 35.5 92-93 26.167005247340704 33.0 23.5 35.0 2.0 35.0 94-95 25.875207819392678 33.0 22.0 35.0 2.0 35.0 96-97 25.510211255339968 32.5 20.0 35.0 2.0 35.0 98-99 25.156411604318208 32.0 18.5 35.0 2.0 35.0 100-101 24.72369521089116 32.0 16.5 35.0 2.0 35.0 102-103 24.3019279306097 32.0 8.5 34.5 2.0 35.0 104-105 23.884674091690705 31.0 6.0 34.0 2.0 35.0 106-107 23.416512022648753 31.0 2.0 34.0 2.0 35.0 108-109 22.978857048758783 31.0 2.0 34.0 2.0 35.0 110-111 22.506812517615167 31.0 2.0 34.0 2.0 35.0 112-113 21.94137672232796 30.0 2.0 34.0 2.0 35.0 114-115 21.378678601173604 29.5 2.0 34.0 2.0 35.0 116-117 20.64709733706168 29.0 2.0 34.0 2.0 35.0 118-119 19.830954559992904 28.5 2.0 34.0 2.0 35.0 120-121 18.32918512630669 25.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6605587466753171 1101 2 0.7117582940671277 1101 3 0.6582856736726157 1101 4 0.8624379258865744 1101 5 1.0065037370669714 1101 6 1.0162442264070108 1101 7 0.8803316843824973 1101 8 0.807060883522638 1101 9 0.9667761083412572 1101 10-11 1.0661926921257745 1101 12-13 1.122657999169185 1101 14-15 1.2976425528785 1101 16-17 1.2905084428809772 1101 18-19 0.9437262086893483 1101 20-21 1.2434199061939708 1101 22-23 1.0827319143805951 1101 24-25 1.1113482264344299 1101 26-27 1.2208747763904384 1101 28-29 1.225035934687078 1101 30-31 1.0637851098949582 1101 32-33 1.1487964803310504 1101 34-35 1.2103851089390858 1101 36-37 1.2434493044871218 1101 38-39 1.132888388180909 1101 40-41 1.3394099408027174 1101 42-43 1.1306771030535145 1101 44-45 1.3764321428141528 1101 46-47 1.4454753720382314 1101 48-49 1.4151765351825105 1101 50-51 1.2822753955373365 1101 52-53 1.218419199392848 1101 54-55 1.3057129689255689 1101 56-57 1.3165161739456614 1101 58-59 1.2174212696620295 1101 60-61 1.4461642753114567 1101 62-63 1.4396572329044126 1101 64-65 1.3994004854099913 1101 66-67 1.3607409712040663 1101 68-69 1.3245179631120259 1101 70-71 1.1023361366811173 1101 72-73 0.7684199659057285 1101 74-75 0.8801182122928175 1101 76-77 0.8832057155787396 1101 78-79 1.271242476000829 1101 80-81 1.4103876451323423 1101 82-83 1.2170280137735645 1101 84-85 1.2399548732439527 1101 86-87 1.4650590863963302 1101 88-89 1.6610589838389878 1101 90-91 1.6439134794861268 1101 92-93 1.5909999205389198 1101 94-95 1.572915614067906 1101 96-97 1.7336718598256802 1101 98-99 1.9553215460333533 1101 100-101 2.3287377946141063 1101 102-103 2.2507199895454626 1101 104-105 2.312944455049081 1101 106-107 2.378195646391692 1101 108-109 2.458138099275587 1101 110-111 2.6797350146517687 1101 112-113 2.5659244137732635 1101 114-115 2.6086032593486728 1101 116-117 2.5705603355224653 1101 118-119 2.612234768909815 1101 120-121 2.532999978062904 1102 1 0.9298679072402862 1102 2 0.8097048765124129 1102 3 0.9697763613741088 1102 4 1.2045647872415373 1102 5 1.2836910262052612 1102 6 1.1382164446709382 1102 7 0.8936575237207123 1102 8 1.1850745147157937 1102 9 1.1407150782909667 1102 10-11 1.1437974967564273 1102 12-13 1.1272641250136957 1102 14-15 1.3341392855926202 1102 16-17 1.277643861590569 1102 18-19 1.1874856951388395 1102 20-21 1.4407703287490463 1102 22-23 1.4435772131978624 1102 24-25 1.5964228792372097 1102 26-27 1.6201494741770475 1102 28-29 1.712698150801124 1102 30-31 1.5691069553044912 1102 32-33 1.3632494476502472 1102 34-35 1.455977711333162 1102 36-37 1.6597843436642634 1102 38-39 1.4998216477659128 1102 40-41 1.5695327724802652 1102 42-43 1.5608434290313795 1102 44-45 1.7366647479489643 1102 46-47 1.7685089306713735 1102 48-49 1.7065853681996472 1102 50-51 1.446113112146147 1102 52-53 1.6997548815508026 1102 54-55 1.753342057873894 1102 56-57 1.6649266211177185 1102 58-59 1.689840123746098 1102 60-61 2.117082724987906 1102 62-63 1.8473939553507002 1102 64-65 1.7380924886651172 1102 66-67 1.6566097077889808 1102 68-69 1.6830899896236886 1102 70-71 1.6183282362095852 1102 72-73 1.5912112275234485 1102 74-75 1.6915972320027848 1102 76-77 1.3328114119418508 1102 78-79 1.6289143293793558 1102 80-81 1.7405232867750549 1102 82-83 1.8037983981271353 1102 84-85 1.4941542330436022 1102 86-87 1.669177937054478 1102 88-89 1.7411877984653863 1102 90-91 1.7603034326999634 1102 92-93 1.8362149268669405 1102 94-95 1.7960282171372342 1102 96-97 1.6641979074996023 1102 98-99 1.7591220343015586 1102 100-101 1.990309934826623 1102 102-103 1.8631410878559862 1102 104-105 2.159237285887535 1102 106-107 2.3853071630965346 1102 108-109 2.602915282840897 1102 110-111 2.6338490243347117 1102 112-113 2.694585121985593 1102 114-115 2.944177674571282 1102 116-117 3.2924398553175855 1102 118-119 3.3199704486845185 1102 120-121 3.2720840754841767 1103 1 0.6247119970385597 1103 2 0.713982752826972 1103 3 0.9444120879222453 1103 4 1.1575562734047793 1103 5 1.1371465623984491 1103 6 1.1493797781207107 1103 7 0.956665219939353 1103 8 0.79573110605034 1103 9 1.0362954125053108 1103 10-11 1.1903173155867464 1103 12-13 1.1521878937674828 1103 14-15 1.497751204187871 1103 16-17 1.3917546307337645 1103 18-19 1.1786620173321225 1103 20-21 1.3760510680207219 1103 22-23 1.52433899524312 1103 24-25 1.1544019652902193 1103 26-27 1.3083268067496832 1103 28-29 1.5350977823554928 1103 30-31 1.2604211858702143 1103 32-33 1.500227650106467 1103 34-35 1.4424006579408868 1103 36-37 1.422442546948453 1103 38-39 1.446364365078331 1103 40-41 1.4864533276843659 1103 42-43 1.5640058775906525 1103 44-45 1.4237644287932838 1103 46-47 1.4277141851614843 1103 48-49 1.6863331485346365 1103 50-51 1.377870137258153 1103 52-53 1.4228996193631787 1103 54-55 1.444305049010758 1103 56-57 1.3925928388706303 1103 58-59 1.4818384626358743 1103 60-61 1.502004268578471 1103 62-63 1.4992108916890423 1103 64-65 1.319410561518513 1103 66-67 1.4802965703993785 1103 68-69 1.6354614702757644 1103 70-71 1.6377670422801387 1103 72-73 1.5833532964336463 1103 74-75 1.4241164402069089 1103 76-77 1.367700548358588 1103 78-79 1.6553730726537381 1103 80-81 1.7811548320862371 1103 82-83 1.614509454212893 1103 84-85 1.7169490251266808 1103 86-87 2.0482448433143006 1103 88-89 2.068203026602834 1103 90-91 1.9521988577902292 1103 92-93 1.8583481856831803 1103 94-95 1.9902196355106128 1103 96-97 2.0022542144789846 1103 98-99 1.9681767668485826 1103 100-101 2.1406918349349056 1103 102-103 2.202479451093186 1103 104-105 2.2387383577176116 1103 106-107 2.2928500136600043 1103 108-109 2.5501012922564428 1103 110-111 2.691658312321291 1103 112-113 2.520382331993133 1103 114-115 2.5605459494358627 1103 116-117 2.3317857866971714 1103 118-119 2.7059495916603105 1103 120-121 2.814152920999632 1104 1 0.5958193375347705 1104 2 0.6763418147961247 1104 3 0.7283849085036032 1104 4 1.1809208498982215 1104 5 1.1645070867721827 1104 6 1.1004715454608558 1104 7 0.8215245095012733 1104 8 0.8945925611332086 1104 9 0.9095330212898105 1104 10-11 1.1295776487825506 1104 12-13 0.9615290756615593 1104 14-15 1.148137240257931 1104 16-17 1.1354202882444255 1104 18-19 1.1605553179695747 1104 20-21 1.4952205771757576 1104 22-23 0.964144098357167 1104 24-25 1.2469421523617896 1104 26-27 1.340371383940436 1104 28-29 1.1224566022046858 1104 30-31 1.314571799983291 1104 32-33 1.4678852653604082 1104 34-35 1.7134389458234622 1104 36-37 1.4022889654130992 1104 38-39 1.3607512301671463 1104 40-41 1.2179548782206169 1104 42-43 1.2711249793062223 1104 44-45 1.381585678403475 1104 46-47 1.4463477375649632 1104 48-49 1.468769960066524 1104 50-51 1.2710695685667837 1104 52-53 1.4783659133588003 1104 54-55 1.6561890716112018 1104 56-57 1.7582119008785249 1104 58-59 1.5634987470202937 1104 60-61 1.18282139440144 1104 62-63 1.6946656220351919 1104 64-65 1.4823495492246295 1104 66-67 1.3813595738878455 1104 68-69 1.456396808749787 1104 70-71 1.2737769633877676 1104 72-73 1.3308173319342451 1104 74-75 1.506626081423864 1104 76-77 1.4004913392773481 1104 78-79 1.7328253120697035 1104 80-81 1.7333695518178516 1104 82-83 1.6848995385287466 1104 84-85 1.845991702159953 1104 86-87 1.7614808637918387 1104 88-89 2.042236628979868 1104 90-91 2.228495022803706 1104 92-93 2.161651864163364 1104 94-95 2.088912575098778 1104 96-97 1.7509177122795627 1104 98-99 2.1540629162307887 1104 100-101 2.180945247373675 1104 102-103 2.5309979868291244 1104 104-105 2.411540344263493 1104 106-107 2.2393323011319417 1104 108-109 2.2253428795561874 1104 110-111 2.181361918746571 1104 112-113 2.3003974596484618 1104 114-115 2.4812787787722783 1104 116-117 2.0978969333192126 1104 118-119 2.226578212564366 1104 120-121 2.293439558108293 1105 1 0.42610850874404704 1105 2 0.6609174149006343 1105 3 0.532309454670731 1105 4 0.7997146336886445 1105 5 0.8782054813572273 1105 6 0.5675291630866965 1105 7 0.8325540608381488 1105 8 0.9750776916722685 1105 9 1.1395501942498782 1105 10-11 1.0577549255396548 1105 12-13 1.1184215961563382 1105 14-15 1.3786166762523706 1105 16-17 1.0754835311870963 1105 18-19 0.6259056887849184 1105 20-21 1.0441802620329597 1105 22-23 0.8429206421928797 1105 24-25 1.0882686909495831 1105 26-27 1.1715102705341351 1105 28-29 0.9043745248348003 1105 30-31 1.3146856908758124 1105 32-33 1.1032155601145064 1105 34-35 0.9568248997266764 1105 36-37 1.3863542896560048 1105 38-39 1.1461204934717273 1105 40-41 0.9230221296760774 1105 42-43 1.217520332557804 1105 44-45 1.053354359301288 1105 46-47 1.3564576757735693 1105 48-49 1.350819192196262 1105 50-51 1.085581501429214 1105 52-53 1.4728142906368902 1105 54-55 1.3272062959338342 1105 56-57 1.2864981555876902 1105 58-59 1.3488467953546994 1105 60-61 1.178345035694253 1105 62-63 1.3705857376232835 1105 64-65 1.6122320629529412 1105 66-67 1.9469305803930226 1105 68-69 1.8202815600991755 1105 70-71 1.5080348972283275 1105 72-73 1.2385690587211613 1105 74-75 1.0833159654561335 1105 76-77 1.1716867693494564 1105 78-79 1.627537648239887 1105 80-81 1.547258434503533 1105 82-83 1.7079602111102972 1105 84-85 1.751011164103648 1105 86-87 1.38785844113157 1105 88-89 1.4185678683584264 1105 90-91 1.5711156745722512 1105 92-93 1.8145057242830163 1105 94-95 1.7625113263186378 1105 96-97 1.4155481140017763 1105 98-99 1.2895149179190533 1105 100-101 1.3596311251263167 1105 102-103 1.3959166888302512 1105 104-105 1.4814336217977306 1105 106-107 1.3495664027449514 1105 108-109 1.6482398618941652 1105 110-111 1.3000038480136276 1105 112-113 1.624903876618653 1105 114-115 1.8559641830961127 1105 116-117 2.0050311928162614 1105 118-119 1.8287070789926823 1105 120-121 1.5589102959649566 1106 1 0.727826509053596 1106 2 0.6597875588412023 1106 3 0.7812492472724948 1106 4 0.8995753363662473 1106 5 0.801571317399123 1106 6 0.905118803493238 1106 7 0.9882936264368709 1106 8 0.9428381002777471 1106 9 0.7534386532087609 1106 10-11 0.8821887077199122 1106 12-13 1.1860246503791103 1106 14-15 1.0057925520197912 1106 16-17 0.9556826747665284 1106 18-19 0.7571289256419576 1106 20-21 1.0547797564352734 1106 22-23 0.6004968687831891 1106 24-25 1.15432141756569 1106 26-27 1.1631395332901633 1106 28-29 1.1332451846913827 1106 30-31 0.8827866040471477 1106 32-33 1.062705835097745 1106 34-35 1.2715723588403378 1106 36-37 0.7526546559563698 1106 38-39 0.6145077400279817 1106 40-41 0.6654974946866545 1106 42-43 0.9134084819670818 1106 44-45 0.7206633413990033 1106 46-47 1.051757680932731 1106 48-49 1.3576782955342352 1106 50-51 1.1880862736522992 1106 52-53 1.037391497467624 1106 54-55 0.7965402483663766 1106 56-57 0.8885386034027825 1106 58-59 0.9191790452644177 1106 60-61 0.8438689484012656 1106 62-63 0.9439954265259303 1106 64-65 0.7770491707278033 1106 66-67 0.8704898849382445 1106 68-69 0.8545512809886127 1106 70-71 0.6769870718143025 1106 72-73 0.8312456309752001 1106 74-75 0.4128598957042904 1106 76-77 0.5885288474591412 1106 78-79 0.5954992640888292 1106 80-81 0.3360346817304851 1106 82-83 0.4398954120595846 1106 84-85 0.5436438834969302 1106 86-87 0.6566016697339556 1106 88-89 0.9035701899804671 1106 90-91 0.371087299191764 1106 92-93 0.7405001523901191 1106 94-95 0.4459787978142806 1106 96-97 0.5103089869316619 1106 98-99 0.4859576770378702 1106 100-101 0.8734009234031568 1106 102-103 1.266837082990083 1106 104-105 0.9158091054689876 1106 106-107 0.9866814004233291 1106 108-109 0.9398381696896578 1106 110-111 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88-89 -0.4253201725305864 2316 90-91 -0.6011275549196426 2316 92-93 -0.8328641270086408 2316 94-95 -0.6988487883926204 2316 96-97 -0.8909951686828386 2316 98-99 -0.29215903383746067 2316 100-101 -0.2281021712033855 2316 102-103 -0.6299135801398918 2316 104-105 -0.7224534940005078 2316 106-107 -0.862036548294622 2316 108-109 -0.904967312184791 2316 110-111 -1.0453354290279329 2316 112-113 -0.9262536432129806 2316 114-115 -0.9325860840238498 2316 116-117 -0.5659695241290876 2316 118-119 -0.03798734039721907 2316 120-121 -0.643774201826389 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11559.0 3 1638.0 4 1427.0 5 1525.0 6 1863.0 7 2145.0 8 2620.0 9 3253.0 10 4524.0 11 5666.0 12 5731.0 13 6439.0 14 7936.0 15 7712.0 16 5937.0 17 5782.0 18 6038.0 19 6372.0 20 7006.0 21 7522.0 22 8325.0 23 9192.0 24 10380.0 25 11752.0 26 13455.0 27 15060.0 28 17778.0 29 20476.0 30 23848.0 31 27846.0 32 33202.0 33 39148.0 34 48289.0 35 60989.0 36 78049.0 37 82295.0 38 28654.0 39 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.24604010148808 18.800459340263785 13.129900598384664 21.823599959863472 2 14.7857113129444 21.61263161938667 39.25483178009918 24.34682528756975 3 20.32329315343479 26.60599725741027 30.274930236572338 22.795779352582603 4 11.680457238571833 16.692532313411725 40.871930685394354 30.75507976262209 5 11.36832245916994 38.05435057109337 35.14533870807906 15.431988261657626 6 26.696277874386254 37.330420161620296 19.617877966448447 16.355423997545007 7 25.076360707041808 32.82559390329082 24.308518034872588 17.78952735479478 8 23.29212055337548 32.25226377244791 21.89205821471672 22.56355745945989 9 23.4831457081744 18.823316888822102 24.677317764868 33.01621963813549 10-11 23.418091357687175 25.43076469276315 29.14571297701869 22.00543097253098 12-13 26.419777760012792 23.652889919258136 28.550483651770726 21.376848668958353 14-15 22.58133886757941 25.478184123471674 29.084131306170548 22.85634570277837 16-17 20.020001534889968 28.801890472810065 29.423121198749065 21.754986793550906 18-19 19.27929857995696 29.568775041008898 30.51837486450276 20.63355151453138 20-21 20.90554061022261 27.67539003321728 29.17916145356576 22.239907902994354 22-23 20.01651608199523 27.56618823856705 29.620865583395023 22.796430096042698 24-25 21.47150484420047 27.299656052971997 29.652838962114657 21.57600014071287 26-27 21.164272447652195 25.88930704769489 31.778710040048903 21.167710464604017 28-29 19.772087471207115 28.442054043076798 30.850942199956343 20.934916285759744 30-31 20.46682812898542 27.77029279904178 29.844249364132175 21.918629707840623 32-33 20.56107500191919 27.476953734742445 30.375009595946672 21.586961667391694 34-35 21.67312268894661 26.29690878616119 30.441611116086854 21.588357408805354 36-37 21.73601870569871 26.592367384016992 30.992348831853423 20.67926507843087 38-39 21.21886487381223 27.790416590367332 30.670733121321142 20.3199854144993 40-41 20.372008149922756 28.322181031918397 30.416835923631258 20.88897489452759 42-43 19.785192310613965 28.602270737329956 28.950501636405363 22.66203531565072 44-45 20.476708731396933 27.69171225389494 28.73542721383602 23.096151800872107 46-47 21.40505966709265 28.26201698924697 28.793590725809675 21.539332617850704 48-49 21.351397605932068 27.628068559144737 29.36515933795257 21.655374496970627 50-51 19.895733375804028 27.994484145118527 30.548808352854646 21.560974126222803 52-53 20.052932947600542 28.815343356307206 29.053053722382767 22.078669973709488 54-55 19.813939650353063 28.337173600608473 29.24988023324725 22.599006515791217 56-57 20.865594901586057 27.335207123066407 28.90375304357561 22.89544493177193 58-59 21.953747681350183 26.524554711344585 28.86354391189091 22.658153695414327 60-61 21.35999027710258 27.23154888314612 30.304256326279965 21.10420451347133 62-63 20.12731882428898 27.436030571238618 30.197778600933013 22.238872003539388 64-65 19.70845079887233 28.494366454082044 29.884833105602187 21.912349641443445 66-67 20.065907901747785 27.239852599273878 29.11500360821361 23.579235890764732 68-69 19.693318939820124 27.856409501319185 28.998661211215737 23.451610347644955 70-71 21.10445781511313 27.196148350345695 28.532547753150972 23.166846081390208 72-73 20.17565781569146 26.397693139492972 30.653290079698824 22.773358965116746 74-75 19.505400099166696 28.690735283398066 29.596612632299024 22.20725198513621 76-77 20.315693863297042 27.616765239233377 28.800196717950033 23.267344179519547 78-79 20.727438940581937 28.089809525940463 28.40985005641663 22.772901477060962 80-81 21.417643162848965 27.254489805212078 28.3427168045226 22.985150227416355 82-83 20.755875790702696 26.019496669252362 30.025590378017863 23.19903716202708 84-85 21.003823893014157 26.77068956417855 29.27884992139509 22.9466366214122 86-87 21.7624652216577 26.990232873940656 28.369930399330144 22.8773715050715 88-89 20.458440805390598 26.871573390918364 28.13604462839662 24.533941175294423 90-91 20.920005439086236 26.045704321673956 28.610073507650714 24.424216731589095 92-93 21.787122333750062 27.074309856496225 28.253470814126576 22.885096995627137 94-95 20.750243949258554 27.127317517956268 28.59325260345848 23.5291859293267 96-97 19.748923454925873 27.80845495786598 29.37704351609539 23.06557807111276 98-99 21.522602543615925 28.24258245317696 27.600360220291027 22.634454782916087 100-101 21.28973329046273 26.706066030326152 28.126154746879482 23.87804593233163 102-103 22.392641226185038 25.815693140219643 28.646815167673477 23.14485046592184 104-105 23.091036770786843 26.09650911871364 28.20450680884214 22.60794730165738 106-107 22.618987632340673 25.95400859169608 29.01469034461963 22.412313431343613 108-109 22.710905144074726 25.803396749780443 28.342800742889047 23.142897363255788 110-111 22.489494067567502 26.56501200563729 27.244398302414087 23.70109562438112 112-113 22.86533107134773 26.28686424647005 27.23557044349475 23.61223423868747 114-115 22.46652696239182 25.838545582518833 27.616175835426233 24.078751619663112 116-117 23.056614819601645 26.43016641820171 26.921951079063046 23.5912676831336 118-119 23.82134987382675 25.45652321707798 27.661575745941857 23.060551163153413 120-121 23.55160131531902 27.228799439445652 26.73751450778321 22.48208473745212 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1850.0 1 1733.5 2 1194.5 3 707.5 4 602.5 5 551.5 6 546.5 7 595.0 8 669.5 9 694.5 10 668.5 11 659.5 12 673.5 13 704.5 14 777.0 15 875.0 16 924.0 17 990.5 18 1141.0 19 1255.0 20 1361.0 21 1517.5 22 1696.0 23 2000.5 24 2384.0 25 2887.0 26 3611.5 27 4534.5 28 5694.0 29 7104.5 30 8593.5 31 9932.5 32 11228.5 33 12486.5 34 13719.0 35 14897.0 36 15932.0 37 17391.5 38 18686.5 39 19237.0 40 19898.5 41 20437.0 42 20849.5 43 21834.5 44 22466.0 45 23020.5 46 25251.0 47 30405.0 48 41447.0 49 41509.0 50 30811.0 51 24514.5 52 19407.5 53 16317.0 54 14496.0 55 12964.0 56 11254.5 57 9460.5 58 7832.5 59 6357.5 60 5119.5 61 4065.0 62 3114.0 63 2244.0 64 1543.0 65 1023.5 66 678.5 67 434.5 68 245.0 69 180.0 70 134.0 71 92.0 72 67.5 73 52.0 74 45.0 75 44.0 76 32.0 77 18.5 78 17.0 79 19.0 80 17.5 81 14.5 82 11.0 83 6.5 84 5.5 85 5.5 86 3.0 87 1.5 88 2.0 89 1.5 90 0.5 91 0.0 92 0.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.586011102701573 2 1.0830359206913696 3 0.9297641705116553 4 0.9324559264548942 5 0.9367310682470968 6 0.9332476193793762 7 0.9356226981528221 8 0.9410062100392996 9 0.9531982810763223 10-11 0.9613527181984869 12-13 0.9671320765472055 14-15 0.9688738009810658 16-17 0.9668153993774126 18-19 0.9633319505096919 20-21 0.9706946947073745 22-23 0.9669737379623091 24-25 0.9771074073956786 26-27 0.9813033798954331 28-29 0.9795616554615728 30-31 0.9866868917819108 32-33 0.9965830533379356 34-35 0.9978497620171068 36-37 0.9965830533379356 38-39 0.994287143856938 40-41 0.9937329588098006 42-43 0.9903286792345278 44-45 0.9961872068756946 46-47 1.0037082896582736 48-49 1.0048166597525485 50-51 1.0788399481916149 52-53 1.017721254421605 54-55 1.0109126952710596 56-57 0.9920704036683884 58-59 0.9380769462187163 60-61 0.9864493839045662 62-63 1.0436887823446144 64-65 1.0374344082412068 66-67 1.044242967391752 68-69 1.0671228929092815 70-71 1.1568217012530917 72-73 1.1610968430452944 74-75 1.1648177997903597 76-77 1.1580884099322628 78-79 0.9272307531533128 80-81 0.90625089065454 82-83 1.0011748722999312 84-85 0.9942079745644897 86-87 1.0052125062147894 88-89 1.0126544197049203 90-91 1.0217588883364632 92-93 1.021679719044015 94-95 1.0178004237140532 96-97 1.0149503291859179 98-99 1.010991864563508 100-101 1.0166920536197783 102-103 1.0132877740445059 104-105 1.0157420221104 106-107 1.0164545457424339 108-109 1.0182754394687423 110-111 1.0189087938083279 112-113 1.0202546717799474 114-115 1.0178004237140532 116-117 1.0111502031484043 118-119 1.017404577251812 120-121 1.023263104892979 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 631558.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.4183467771813 #Duplication Level Percentage of deduplicated Percentage of total 1 88.11987016250646 61.17135704901065 2 7.704604426201987 10.69681803678191 3 1.7413948742247936 3.6265425976482817 4 0.744975266267649 2.0685980549676253 5 0.4309810166040626 1.4958994832501473 6 0.2653040428218215 1.105018082759602 7 0.18765585831749476 0.9118731613217418 8 0.13780524547630787 0.7652969854551148 9 0.10216466578011388 0.6382891977748897 >10 0.5166526752524797 6.25062295474766 >50 0.024525119528462674 1.1497392950322467 >100 0.016732049831867803 2.404805595037498 >500 0.0036672985932316667 1.7804535270787223 >1k 0.0032088862690777087 3.7975053021258143 >5k 4.5841232415395834E-4 2.137180677008168 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6752 1.0691021252204864 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6680 1.0577017471079457 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4256 0.6738890173190744 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2708 0.4287808878994486 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1960 0.3103436263969422 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1708 0.2704423030030496 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1651 0.26141700366395487 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1480 0.2343411056466706 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1450 0.22959094809977865 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1431 0.22658251498674709 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1388 0.2197739558362019 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1323 0.20948194781793597 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1218 0.19285639640381408 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1107 0.17528081348031377 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1105 0.17496413631052096 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1082 0.1713223488579038 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 924 0.14630485244427274 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 884 0.1399713090484168 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 878 0.13902127753903837 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 851 0.1347461357468356 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 836 0.13237105697338963 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 823 0.13031265536973644 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 680 0.10767023772955135 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 640 0.10133669433369541 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 639 0.101178355748799 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 632 0.10006998565452421 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5833858489639906E-4 0.0 5 0.0 0.0 0.0 1.5833858489639906E-4 0.0 6 0.0 0.0 0.0 1.5833858489639906E-4 0.0 7 0.0 0.0 0.0 1.5833858489639906E-4 0.0 8 0.0 0.0 0.0 1.5833858489639906E-4 0.0 9 0.0 0.0 0.0 1.5833858489639906E-4 0.0 10-11 0.0 0.0 0.0 4.7501575468919717E-4 0.0 12-13 0.0 0.0 0.0 4.7501575468919717E-4 0.0 14-15 0.0 0.0 0.0 6.333543395855962E-4 0.0 16-17 0.0 0.0 0.0 7.125236320337958E-4 0.0 18-19 0.0 0.0 0.0 0.0011083700942747935 0.0 20-21 0.0 0.0 0.0 0.001979232311204988 0.0 22-23 0.0 0.0 0.0 0.004275141792202775 0.0 24-25 0.0 0.0 0.0 0.007916929244819952 0.0 26-27 0.0 0.0 0.0 0.013300441131297521 0.0 28-29 0.0 0.0 0.0 0.028184268111559035 0.0 30-31 0.0 0.0 0.0 0.05367678027987928 0.0 32-33 0.0 0.0 0.0 0.08637369806098569 0.0 34-35 1.5833858489639906E-4 0.0 0.0 0.11511215121968212 0.0 36-37 1.5833858489639906E-4 7.916929244819953E-5 0.0 0.14131718702003615 0.0 38-39 1.5833858489639906E-4 1.5833858489639906E-4 0.0 0.20172335715801243 0.0 40-41 1.5833858489639906E-4 1.5833858489639906E-4 0.0 0.336073646442607 0.0 42-43 1.5833858489639906E-4 1.5833858489639906E-4 0.0 0.5446847320436128 0.0 44-45 1.5833858489639906E-4 1.5833858489639906E-4 0.0 0.6993023601949464 0.0 46-47 1.5833858489639906E-4 1.5833858489639906E-4 0.0 1.0873902317760207 0.0 48-49 1.5833858489639906E-4 1.5833858489639906E-4 0.0 1.3898169289281428 0.0 50-51 1.5833858489639906E-4 1.5833858489639906E-4 0.0 1.572381317313691 0.0 52-53 1.5833858489639906E-4 1.5833858489639906E-4 0.0 1.8286523169685127 0.0 54-55 1.5833858489639906E-4 1.5833858489639906E-4 0.0 2.029979827664284 0.0 56-57 1.5833858489639906E-4 1.5833858489639906E-4 0.0 2.6114782806963097 0.0 58-59 1.5833858489639906E-4 1.5833858489639906E-4 0.0 3.4547895838545313 0.0 60-61 1.5833858489639906E-4 1.5833858489639906E-4 0.0 4.149816802257275 0.0 62-63 1.5833858489639906E-4 1.5833858489639906E-4 0.0 4.683576171943036 0.0 64-65 1.5833858489639906E-4 1.5833858489639906E-4 0.0 5.530133416091633 0.0 66-67 1.5833858489639906E-4 1.5833858489639906E-4 0.0 6.640878589139874 0.0 68-69 1.5833858489639906E-4 1.5833858489639906E-4 0.0 7.6034188467250825 0.0 70-71 1.5833858489639906E-4 1.5833858489639906E-4 0.0 8.087618239338271 0.0 72-73 1.5833858489639906E-4 1.5833858489639906E-4 0.0 8.440317437195 0.0 74-75 1.5833858489639906E-4 1.5833858489639906E-4 0.0 8.924120983345947 0.0 76-77 1.5833858489639906E-4 1.5833858489639906E-4 0.0 9.439592246476174 0.0 78-79 1.5833858489639906E-4 1.5833858489639906E-4 0.0 9.960842867955119 0.0 80-81 1.5833858489639906E-4 1.5833858489639906E-4 0.0 10.836534411724656 0.0 82-83 1.5833858489639906E-4 2.3750787734459858E-4 0.0 11.709771707428295 0.0 84-85 1.5833858489639906E-4 3.166771697927981E-4 0.0 13.14922778272146 0.0 86-87 1.5833858489639906E-4 3.166771697927981E-4 0.0 14.323466728313155 0.0 88-89 1.5833858489639906E-4 3.166771697927981E-4 0.0 15.685653574176877 0.0 90-91 1.5833858489639906E-4 3.166771697927981E-4 0.0 16.591429449076728 0.0 92-93 1.5833858489639906E-4 3.166771697927981E-4 0.0 17.3972145076145 0.0 94-95 1.5833858489639906E-4 3.166771697927981E-4 0.0 18.14005997865596 0.0 96-97 1.5833858489639906E-4 3.166771697927981E-4 0.0 19.105371161476857 0.0 98-99 1.5833858489639906E-4 3.166771697927981E-4 0.0 19.916064716146415 0.0 100-101 1.5833858489639906E-4 3.166771697927981E-4 0.0 20.40010577017471 0.0 102-103 1.5833858489639906E-4 3.166771697927981E-4 0.0 20.98801693589504 0.0 104-105 1.5833858489639906E-4 3.166771697927981E-4 0.0 21.824440510610266 0.0 106-107 1.5833858489639906E-4 3.166771697927981E-4 0.0 22.74882116923545 0.0 108-109 1.5833858489639906E-4 3.166771697927981E-4 0.0 23.754192014035134 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 185 0.0 62.21455 2 ATAACGC 195 0.0 61.84141 3 TAACGCA 215 0.0 50.74694 4 TACCTGG 600 0.0 47.95705 2 TCACGCA 120 1.0913936E-11 47.85347 4 TATCACG 115 3.8016879E-10 45.037926 2 GTATAAC 300 0.0 42.303944 1 ACCTGGG 765 0.0 33.778923 3 TAACGCC 70 0.0033179729 32.81381 4 TACACGG 80 0.0063090664 28.774231 2 CCTGGGG 585 0.0 28.466682 4 AGTCTCG 125 6.214689E-5 27.5636 8 CACGCAG 215 5.869879E-9 26.71319 5 GCCGTTC 110 9.532425E-4 26.101894 8 CTAGATA 135 1.04904175E-4 25.521852 4 GAGTACG 160 1.1102224E-5 25.238148 1 TACGCAG 115 0.0012353964 24.967028 4 GTACCTG 1205 0.0 24.89406 1 GAGTACT 2375 0.0 23.949263 12-13 AGTACTT 2460 0.0 23.355299 12-13 >>END_MODULE