##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003817_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 631558 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44086845547044 31.0 31.0 34.0 30.0 34.0 2 31.63809974697494 31.0 31.0 34.0 30.0 34.0 3 31.687506135620165 31.0 31.0 34.0 30.0 34.0 4 35.47063135927342 37.0 35.0 37.0 33.0 37.0 5 35.32020020330674 37.0 35.0 37.0 33.0 37.0 6 35.34265894818845 37.0 35.0 37.0 33.0 37.0 7 35.267877217927726 37.0 35.0 37.0 32.0 37.0 8 35.263136560695926 37.0 35.0 37.0 32.0 37.0 9 36.79324464261398 39.0 36.0 39.0 32.0 39.0 10-11 36.705487065321 39.0 35.0 39.0 32.0 39.0 12-13 36.63948600128571 39.0 35.0 39.0 32.0 39.0 14-15 37.652376345482125 39.0 36.0 41.0 32.0 41.0 16-17 37.66880555705097 39.0 36.5 41.0 32.0 41.0 18-19 37.70164339617264 39.5 37.0 41.0 32.0 41.0 20-21 37.61199288109722 39.0 36.5 41.0 32.0 41.0 22-23 37.49941493892881 39.0 36.0 41.0 32.0 41.0 24-25 37.38849717682304 39.0 36.0 41.0 32.0 41.0 26-27 37.10551604128204 39.0 36.0 40.5 31.0 41.0 28-29 36.88695733408491 39.0 36.0 40.0 30.5 41.0 30-31 36.61307591701791 38.5 35.0 40.0 30.0 41.0 32-33 36.296226949860504 38.0 35.0 40.0 30.0 41.0 34-35 36.17295323628234 38.0 35.0 40.0 30.0 41.0 36-37 35.89566120609667 38.0 35.0 40.0 29.5 41.0 38-39 35.572638617514144 38.0 34.0 40.0 28.0 41.0 40-41 35.71910497531502 38.0 35.0 40.0 29.0 41.0 42-43 35.539636897957116 38.0 35.0 40.0 27.5 41.0 44-45 35.337537486659976 38.0 34.5 40.0 27.0 41.0 46-47 35.00217082199893 38.0 34.0 40.0 25.5 41.0 48-49 34.68468216695854 38.0 34.0 40.0 24.0 41.0 50-51 34.3947016742722 38.0 33.0 40.0 23.5 41.0 52-53 34.16723246321003 38.0 33.0 40.0 23.0 41.0 54-55 33.93321991012702 38.0 33.0 40.0 22.5 41.0 56-57 33.66198116404194 38.0 33.0 40.0 21.5 41.0 58-59 33.335752219115264 37.0 32.0 40.0 20.0 41.0 60-61 32.880134524461724 37.0 31.5 40.0 18.0 41.0 62-63 32.58129657133628 36.0 31.0 39.0 16.5 41.0 64-65 32.08018028431276 36.0 31.0 39.0 12.5 40.5 66-67 31.707011865893552 35.0 30.5 39.0 9.5 40.0 68-69 31.414685587071972 35.0 30.0 38.5 8.0 40.0 70-71 30.98587065637677 35.0 30.0 37.5 7.0 40.0 72-73 30.59344905772708 35.0 29.5 37.0 6.5 39.5 74-75 30.23145142647231 34.0 29.0 37.0 2.0 39.0 76-77 29.236012369410254 33.5 28.5 35.5 2.0 38.5 78-79 29.579210460480272 34.0 29.0 36.0 2.0 38.5 80-81 29.371064098625936 34.0 29.0 35.5 2.0 37.5 82-83 29.057145186982034 34.0 29.0 35.0 2.0 37.0 84-85 28.70695644738884 34.0 29.0 35.0 2.0 36.5 86-87 28.339906231890026 34.0 28.0 35.0 2.0 36.0 88-89 28.00158813600651 34.0 27.0 35.0 2.0 36.0 90-91 27.703434997260743 33.0 27.0 35.0 2.0 35.0 92-93 27.458250865320366 33.0 26.5 35.0 2.0 35.0 94-95 27.09977943435124 33.0 26.0 35.0 2.0 35.0 96-97 26.669624959227814 33.0 25.0 35.0 2.0 35.0 98-99 26.236754977373415 33.0 25.0 35.0 2.0 35.0 100-101 25.231985819196336 32.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25466047514807144 1101 2 0.27678809028485674 1101 3 0.23701809306190214 1101 4 0.28398612204401275 1101 5 0.23505011208786186 1101 6 0.2889471246559765 1101 7 0.1815163503394288 1101 8 0.2095045623500269 1101 9 0.372098077278622 1101 10-11 0.4596137470684809 1101 12-13 0.4400774894322481 1101 14-15 0.5261145741865505 1101 16-17 0.5818036766106545 1101 18-19 0.2978748607552859 1101 20-21 0.3509757189228324 1101 22-23 0.32734237606348415 1101 24-25 0.43045279180044105 1101 26-27 0.647313618764386 1101 28-29 0.5781873149659802 1101 30-31 0.6942212537173447 1101 32-33 0.6641012470954308 1101 34-35 0.6428308734175872 1101 36-37 0.6858819713711171 1101 38-39 0.7718428607138392 1101 40-41 0.8522350748371395 1101 42-43 0.8293620349907869 1101 44-45 0.9605303056055305 1101 46-47 0.8980148683366806 1101 48-49 1.0226495792246055 1101 50-51 1.0964703844857837 1101 52-53 1.1622755324252978 1101 54-55 1.249781473493016 1101 56-57 1.0200738517481724 1101 58-59 1.0583327010322776 1101 60-61 0.9994130763241884 1101 62-63 0.706937664067322 1101 64-65 0.8312063554591553 1101 66-67 0.9131834799863192 1101 68-69 0.7533602693500931 1101 70-71 0.969613320788806 1101 72-73 0.8269887391455732 1101 74-75 0.6070467759188745 1101 76-77 0.7657426931466205 1101 78-79 0.8161591309516361 1101 80-81 1.1815613857171634 1101 82-83 1.0623219953571095 1101 84-85 0.8609944520877697 1101 86-87 0.8842049551123701 1101 88-89 1.1672084960692963 1101 90-91 1.282547428035837 1101 92-93 1.1714649977223424 1101 94-95 0.9217245504499196 1101 96-97 1.2186180893791452 1101 98-99 0.7853886922043714 1101 100-101 0.9080788648435956 1102 1 0.17350438075749608 1102 2 0.08761519200044887 1102 3 0.14493529699406338 1102 4 0.13148199728488663 1102 5 0.03414318641495129 1102 6 0.20321121946560794 1102 7 0.08728316825968108 1102 8 0.14085158976781997 1102 9 0.3316429163770138 1102 10-11 0.20658888233029415 1102 12-13 0.29568336354603275 1102 14-15 0.33889147176194 1102 16-17 0.3231384551199028 1102 18-19 0.24721765492645176 1102 20-21 0.20780971120805702 1102 22-23 0.26120596582286026 1102 24-25 0.31245414528745385 1102 26-27 0.1232214505685505 1102 28-29 0.4932046158666026 1102 30-31 0.42594557929501775 1102 32-33 0.5548590558366939 1102 34-35 0.49070235868338585 1102 36-37 0.40908990441372595 1102 38-39 0.5569288836019695 1102 40-41 0.4827043217353193 1102 42-43 0.6556097529054199 1102 44-45 0.7925762705409767 1102 46-47 0.8058645468710992 1102 48-49 0.7761332194592114 1102 50-51 0.9276400087814167 1102 52-53 1.0807378698864483 1102 54-55 1.0943174027144806 1102 56-57 1.0706809100389805 1102 58-59 1.0417653943473155 1102 60-61 0.8684443400868389 1102 62-63 0.9195734037708228 1102 64-65 0.7591515427105584 1102 66-67 1.0016166772592854 1102 68-69 1.0253813690508444 1102 70-71 1.1738299839716255 1102 72-73 1.0802695549318209 1102 74-75 1.2372523758596898 1102 76-77 1.317383485835208 1102 78-79 1.5006616053106079 1102 80-81 1.4476966043331174 1102 82-83 1.32223370589956 1102 84-85 1.30469117963818 1102 86-87 1.3270424716275393 1102 88-89 1.379051060604457 1102 90-91 1.3614707157084318 1102 92-93 1.5455802784716006 1102 94-95 1.6167652071939962 1102 96-97 1.7765496672838417 1102 98-99 1.5945296450175235 1102 100-101 1.5269044492237782 1103 1 0.029514973569444436 1103 2 -0.009457554019160597 1103 3 0.10619934495409566 1103 4 0.12452059998587117 1103 5 0.2215058116935822 1103 6 0.16836930308258502 1103 7 0.17580908817708973 1103 8 0.19423610185550189 1103 9 0.22996007199743218 1103 10-11 0.3696862319280214 1103 12-13 0.13097503457893112 1103 14-15 0.12987102225309854 1103 16-17 0.3136216777772418 1103 18-19 0.3426968865206632 1103 20-21 0.33332411849313814 1103 22-23 0.19867532131467414 1103 24-25 0.47580667712215785 1103 26-27 0.13780823646256124 1103 28-29 0.41561304457793824 1103 30-31 0.6172921523495916 1103 32-33 0.7943817065753294 1103 34-35 0.8087260514970183 1103 36-37 0.8125896204466727 1103 38-39 0.7492832486089469 1103 40-41 0.8753925905971371 1103 42-43 0.8472444975355984 1103 44-45 0.9961567134260889 1103 46-47 1.0983988008010357 1103 48-49 1.1689249842853329 1103 50-51 1.3678005827700375 1103 52-53 1.282418514976797 1103 54-55 1.342721300889977 1103 56-57 1.4207841472633689 1103 58-59 1.5774650335345015 1103 60-61 1.3701872768106966 1103 62-63 1.557068115773891 1103 64-65 1.5588849112325214 1103 66-67 1.2059005692767677 1103 68-69 1.467049749869421 1103 70-71 1.6107469297539723 1103 72-73 1.5577158437605974 1103 74-75 1.5948251108828515 1103 76-77 1.8062017171667968 1103 78-79 1.875378770245657 1103 80-81 2.0338870731310266 1103 82-83 1.758162912072578 1103 84-85 1.5774373286180357 1103 86-87 1.4350676780696467 1103 88-89 1.7047978163294033 1103 90-91 1.5716946292272063 1103 92-93 1.48961257179986 1103 94-95 1.307814552776076 1103 96-97 1.7279951824802815 1103 98-99 1.6362304330099242 1103 100-101 1.773408226179626 1104 1 0.013231921773204647 1104 2 -0.02512174718827964 1104 3 -0.11519902373843749 1104 4 0.044506731354353235 1104 5 -0.054611668786705536 1104 6 0.03831989124039836 1104 7 -0.058767385897674274 1104 8 -0.03820487413975826 1104 9 -0.19458566252674814 1104 10-11 -0.12606020455035605 1104 12-13 -0.023190978363835768 1104 14-15 -0.08917297191278095 1104 16-17 -0.27912334278358486 1104 18-19 -0.083609060139338 1104 20-21 -0.1154621825530171 1104 22-23 -0.1655132959478962 1104 24-25 -0.0030983789237666315 1104 26-27 -3.6333168064572874E-4 1104 28-29 0.04091496817262197 1104 30-31 0.11524188049729389 1104 32-33 0.2462192034035624 1104 34-35 0.2570014794198414 1104 36-37 0.05855154369270821 1104 38-39 0.0435161447704715 1104 40-41 -0.06950477959266976 1104 42-43 0.4280181386130977 1104 44-45 0.1931619800195321 1104 46-47 0.2306084069590213 1104 48-49 0.241743047596799 1104 50-51 0.1798554452168446 1104 52-53 0.6461466200684782 1104 54-55 0.5021950676761548 1104 56-57 0.45871611349054575 1104 58-59 0.4541368718835983 1104 60-61 0.4011219760880209 1104 62-63 0.4813596243362177 1104 64-65 0.414080708804736 1104 66-67 0.5268184751134122 1104 68-69 0.3294965561134049 1104 70-71 0.4990332627599585 1104 72-73 0.6609393321088142 1104 74-75 0.6176463701778374 1104 76-77 0.6090090497214717 1104 78-79 0.67348703854557 1104 80-81 0.7235033226734195 1104 82-83 0.848173914003727 1104 84-85 0.8276654056238044 1104 86-87 0.6310725049732433 1104 88-89 0.4173253937932664 1104 90-91 0.5400142004618012 1104 92-93 0.28784383704131145 1104 94-95 0.530685495087603 1104 96-97 0.7612915198831693 1104 98-99 0.6381020203290326 1104 100-101 0.8029415351072657 1105 1 0.01592733956296044 1105 2 -0.02336648755054327 1105 3 -0.13961410467913282 1105 4 0.15530917085260398 1105 5 -0.050152058740188465 1105 6 -0.19941506455430158 1105 7 -0.02941703294756337 1105 8 0.04755496793153924 1105 9 -0.32619887119049196 1105 10-11 -0.03819561412210959 1105 12-13 0.015634650213407042 1105 14-15 -0.08502042064512949 1105 16-17 0.016668053764398394 1105 18-19 -0.12104454988133995 1105 20-21 -0.19402233369292787 1105 22-23 -0.25139596153073995 1105 24-25 -0.14968712337716994 1105 26-27 -0.08952873475391243 1105 28-29 0.2610749960423888 1105 30-31 0.06536101930349503 1105 32-33 0.0035512087541960113 1105 34-35 -0.01559860499340715 1105 36-37 -0.30583120116712337 1105 38-39 0.004191406105412909 1105 40-41 -0.11276322035935493 1105 42-43 -0.15144309047062166 1105 44-45 0.1385468307443034 1105 46-47 0.044930521667239987 1105 48-49 0.13973588810264914 1105 50-51 0.31051621337741864 1105 52-53 0.12923940541541867 1105 54-55 0.1591802640932798 1105 56-57 -0.12449011611796834 1105 58-59 -0.14228628414469568 1105 60-61 0.12471882912633703 1105 62-63 -0.11119012476884294 1105 64-65 -0.31169458669746675 1105 66-67 -0.06081051748814659 1105 68-69 -0.008982710161269836 1105 70-71 -0.03918494772381109 1105 72-73 0.19236316924930463 1105 74-75 -0.14811244464227258 1105 76-77 -0.23768375974352907 1105 78-79 -0.24274621000232344 1105 80-81 -0.43449125969314295 1105 82-83 -0.12370508473900799 1105 84-85 0.07023179173179628 1105 86-87 0.2633444942898322 1105 88-89 0.23534672935379675 1105 90-91 0.41145483092605417 1105 92-93 0.2110467615807785 1105 94-95 0.16034246018562825 1105 96-97 0.14348814022676137 1105 98-99 0.23929296176008208 1105 100-101 0.3950078062077864 1106 1 -0.0467100230744002 1106 2 -0.08874336419502526 1106 3 -0.12470413202268205 1106 4 0.025473748059440027 1106 5 -0.4159623048321208 1106 6 -0.24986133190197535 1106 7 0.03491769335899164 1106 8 -0.22630356789883166 1106 9 -0.3603473518178504 1106 10-11 -0.44914851099049 1106 12-13 -0.7249653601049104 1106 14-15 -0.2804390864863322 1106 16-17 -0.45446128637902206 1106 18-19 -0.186166048101434 1106 20-21 -0.4465657996166783 1106 22-23 -0.24117566539494106 1106 24-25 -0.35985582791207094 1106 26-27 -0.12260926924290061 1106 28-29 -0.29663020955013764 1106 30-31 -0.07893552921840552 1106 32-33 -0.13046055413079927 1106 34-35 -0.10183912503674009 1106 36-37 -0.45733251675295605 1106 38-39 -0.2683567030624161 1106 40-41 -0.19409995873834873 1106 42-43 0.17855742544539055 1106 44-45 0.5299800454169556 1106 46-47 0.2877799257842568 1106 48-49 0.1476268234583813 1106 50-51 0.41633058679742163 1106 52-53 0.521477449061905 1106 54-55 0.2468292348408454 1106 56-57 0.18097343441601055 1106 58-59 0.35440153507833116 1106 60-61 0.5147435930947637 1106 62-63 0.4083837286078271 1106 64-65 0.11001257937589415 1106 66-67 0.2590419305333178 1106 68-69 0.4223591873778112 1106 70-71 0.5171840354057373 1106 72-73 0.3429151993788011 1106 74-75 0.4231174991228812 1106 76-77 0.29067614326426394 1106 78-79 0.42496260700226784 1106 80-81 0.6526546929457666 1106 82-83 0.3814076429089326 1106 84-85 0.5630192859277408 1106 86-87 0.3933708060062848 1106 88-89 0.5534889158058398 1106 90-91 0.8166939579961685 1106 92-93 1.0427472207460262 1106 94-95 0.7461754065819548 1106 96-97 1.1254310799161793 1106 98-99 0.6061659948303024 1106 100-101 0.7435787188632084 1107 1 -0.26055617692055577 1107 2 -0.47028182573348687 1107 3 -0.5206015679201172 1107 4 -0.4658083032226159 1107 5 -0.19596230483212196 1107 6 -0.26319466523531077 1107 7 -0.5814925630512633 1107 8 -0.45399587559113286 1107 9 -0.3506037620742575 1107 10-11 -0.49427671611869783 1107 12-13 -0.3400935652331185 1107 14-15 -0.01582370187094284 1107 16-17 -0.9057433376610717 1107 18-19 -0.7530891250245091 1107 20-21 -0.974514517565396 1107 22-23 -0.7586115628308434 1107 24-25 -0.37370198175822367 1107 26-27 -0.8451733718070074 1107 28-29 -0.7299635428834677 1107 30-31 -0.6912432215261006 1107 32-33 -0.5650759387461832 1107 34-35 -0.6372237404213621 1107 36-37 -0.6319479013683349 1107 38-39 -0.4155361902419017 1107 40-41 0.013848759210368655 1107 42-43 -0.6788784719905152 1107 44-45 -0.29078918535228127 1107 46-47 -0.7637585357542065 1107 48-49 -1.0444244585929 1107 50-51 -0.9800796696128273 1107 52-53 -1.1500610124765558 1107 54-55 -0.8762476882360772 1107 56-57 -1.014411180968608 1107 58-59 -1.2594446187678194 1107 60-61 -1.1906410222898494 1107 62-63 -1.0864880662639642 1107 64-65 -0.8507566513933362 1107 66-67 -1.0927529412615549 1107 68-69 -1.5591792741606518 1107 70-71 -1.3597390415173436 1107 72-73 -1.4542642878006866 1107 74-75 -1.4432927572873737 1107 76-77 -1.1898366772485574 1107 78-79 -1.4776014955618315 1107 80-81 -1.1681145378234667 1107 82-83 -1.4591051776038917 1107 84-85 -1.4523653294568746 1107 86-87 -0.7250907324552536 1107 88-89 -1.0390751867582573 1107 90-91 -1.0317675804653739 1107 92-93 -0.8682784202796121 1107 94-95 -0.8717733113667627 1107 96-97 -0.8920048175197159 1107 98-99 -0.8994750308107236 1107 100-101 -0.7966776913931994 1108 1 -0.46091322601480655 1108 2 -0.09619938939848893 1108 3 -0.47343958314721135 1108 4 -0.3965617818259233 1108 5 -0.3207929690484548 1108 6 -0.5295322866582524 1108 7 -0.4123563068160294 1108 8 -0.4700420819445128 1108 9 -0.5441138697140602 1108 10-11 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3359.0 13 4331.0 14 4685.0 15 5267.0 16 6391.0 17 7879.0 18 6945.0 19 5387.0 20 4910.0 21 4869.0 22 5141.0 23 5634.0 24 6460.0 25 7684.0 26 9357.0 27 11137.0 28 13772.0 29 16845.0 30 20539.0 31 24298.0 32 29559.0 33 35984.0 34 45070.0 35 57917.0 36 77501.0 37 100638.0 38 87353.0 39 17902.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.890061481904034 19.772453571654943 13.638207517271978 20.699277429169044 2 15.569728887523631 23.071161878944686 38.594496679576785 22.764612553954898 3 20.947542458238576 27.445189122075753 30.19175097079076 21.415517448894914 4 12.396169472954186 17.853942155748165 39.61441387806029 30.135474493237357 5 11.91054503307693 38.96712574300381 34.1921090382831 14.930220185636156 6 27.00685776355521 38.53854107453602 18.632342485990748 15.822258675918025 7 26.025163167911735 33.211043166265014 23.298572735995744 17.465220929827506 8 24.38176699527201 32.87409865760548 21.086741043577945 21.657393303544566 9 24.135392157173214 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30.606749650451526 20.398667361277152 34-35 22.154607685255122 26.514161841015582 30.808951721320433 20.52227875240887 36-37 22.20074560208794 26.70128088990808 31.56234361043716 19.535629897566817 38-39 21.634443711634706 27.87732289910793 30.929998899465804 19.558234489791555 40-41 20.5051727113939 28.398684375133616 30.637368623582116 20.458774289890368 42-43 20.44721149918139 28.62595359412754 29.110786341080313 21.81604856561076 44-45 21.200348978241113 27.75073706611269 29.063284765611392 21.985629190034803 46-47 22.046896870586295 28.354651052321568 28.758890739696874 20.83956133739526 48-49 22.02576802130604 27.91271743846171 29.295092453899723 20.766422086332533 50-51 20.76468036189867 28.216094167123206 30.499732407791523 20.519493063186594 52-53 20.825957394253575 29.039850655046727 28.885233026895392 21.248958923804306 54-55 20.329645099895814 28.801471915485198 29.03628803688656 21.832594947732435 56-57 21.894011128388158 27.567778977670386 28.688851660549325 21.84935823339213 58-59 22.50227217308417 27.08176972990433 28.705383862662575 21.710574234348922 60-61 21.56349960411718 27.718923198733176 30.08424386381631 20.633333333333333 62-63 20.627625863278535 27.918439677001988 29.87367350849382 21.580260951225654 64-65 20.026568167469758 28.957797487826888 29.406066002172032 21.609568342531325 66-67 20.676976342335294 27.184536753560845 28.898874055443546 23.239612848660318 68-69 20.253855051489094 28.471921481011147 28.48933918040224 22.784884287097523 70-71 21.616514120272694 27.800600579678935 27.907637717290534 22.675247582757837 72-73 20.63658444671748 27.14406277808214 30.115603000832863 22.103749774367518 74-75 19.935698700673573 29.071835049195798 29.073972620091897 21.91849363003873 76-77 20.453307534699903 27.841385905965883 28.308405562117812 23.396900997216406 78-79 20.718366325816472 28.547734333188718 27.78256312167687 22.95133621931794 80-81 21.419172902567933 27.765066708045815 27.70109791974767 23.114662469638574 82-83 20.56066109525966 26.708552500324593 29.142058211597348 23.588728192818394 84-85 19.88249693614838 27.138441758318315 28.550821935594193 24.428239369939103 86-87 20.523900267832474 27.795595450252275 27.669714979696824 24.010789302218424 88-89 20.43581112106885 27.80409716922278 26.952314751772597 24.807776957935772 90-91 20.056115194487283 26.580694093020753 27.87194525285089 25.49124545964108 92-93 20.021755721564766 27.541175949002312 27.614170036639546 24.822898292793376 94-95 19.53955103058708 27.906564327080275 28.128238521433126 24.425646120899522 96-97 18.903816769719185 28.35960446833609 28.8061213987697 23.93045736317502 98-99 19.239937709156866 28.608423514397437 27.12818470644213 25.02345407000357 100-101 18.275218055994294 27.102279879628817 27.584825503743588 27.0376765606333 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 176.0 1 128.5 2 304.5 3 428.5 4 266.0 5 220.0 6 257.5 7 397.5 8 553.0 9 653.0 10 681.0 11 658.0 12 704.0 13 756.0 14 823.5 15 931.5 16 1024.0 17 1090.0 18 1164.0 19 1294.0 20 1427.5 21 1573.0 22 1763.5 23 2057.0 24 2455.5 25 2980.0 26 3634.0 27 4435.0 28 5588.0 29 7070.5 30 8521.0 31 9879.0 32 11301.0 33 12550.5 34 13672.5 35 14830.0 36 15930.5 37 17356.0 38 18753.5 39 19290.0 40 19859.5 41 20472.0 42 20982.5 43 22126.0 44 22695.5 45 23017.5 46 24937.0 47 30871.5 48 43825.5 49 43821.0 50 31944.0 51 25281.0 52 19707.5 53 16313.5 54 14333.0 55 12788.0 56 11101.0 57 9155.0 58 7521.0 59 6119.5 60 4903.0 61 3803.0 62 2875.0 63 2071.0 64 1352.0 65 873.0 66 566.0 67 341.0 68 176.0 69 93.5 70 50.5 71 28.0 72 20.0 73 13.0 74 8.0 75 5.5 76 5.0 77 4.0 78 1.5 79 2.0 80 2.5 81 1.5 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07568584358047875 2 0.0019000630187567889 3 0.01805059867818949 4 0.0 5 0.0 6 0.0017417244338603897 7 0.0 8 0.0 9 0.0 10-11 0.0011083700942747935 12-13 0.0 14-15 0.0028500945281351832 16-17 0.0 18-19 0.0 20-21 0.013933795470883118 22-23 0.0 24-25 0.0 26-27 0.006096035518511364 28-29 0.009262807216439345 30-31 0.008471114291957349 32-33 0.004591818961995573 34-35 0.005858527641166765 36-37 0.019317307357360685 38-39 0.007362744197682556 40-41 0.011242039527644333 42-43 0.0 44-45 0.0 46-47 3.166771697927981E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0031667716979279815 58-59 6.333543395855962E-4 60-61 0.009183637923991145 62-63 0.015675519904743508 64-65 0.05565601259108427 66-67 0.028025929526662635 68-69 0.0026125866507905847 70-71 2.375078773445986E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.001029200801826594 88-89 0.0 90-91 0.0 92-93 0.0 94-95 7.916929244819953E-5 96-97 4.750157546891972E-4 98-99 7.125236320337958E-4 100-101 0.001820893726308589 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 631558.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.85143306312676 #Duplication Level Percentage of deduplicated Percentage of total 1 86.34333394724469 56.85832275874184 2 8.753439133964868 11.528530224048836 3 2.0363073640005274 4.0228127422929845 4 0.8663755910188099 2.2820829695800815 5 0.5035919990847145 1.6581127409426637 6 0.3089465535539386 1.2206743974864538 7 0.22836576343273735 1.0526748949220521 8 0.15761861962207158 0.8303529583636229 9 0.12139310537472603 0.7194518957617978 >10 0.6206416907643465 7.279801689168214 >50 0.03311242302658062 1.5127612105605515 >100 0.01943552251672741 2.7204071780351757 >500 0.0028793366691271184 1.363600740336485 >1k 0.004319005003690677 5.436074144905727 >5k 2.3994472242725985E-4 1.5143394548534033 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9584 1.5175169976470886 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4840 0.7663587508985715 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2572 0.4072468403535384 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2517 0.3985382181842364 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2498 0.39552978507120484 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2141 0.3390029102631904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2126 0.3366278314897444 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2034 0.3220606816792757 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1711 0.27091731875773883 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1706 0.2701256258332568 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1668 0.26410875960719365 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1645 0.2604669721545765 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1503 0.2379828930992878 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1474 0.2333910741372922 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1367 0.2164488455533775 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1263 0.199981632724152 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1167 0.1847811285740977 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1132 0.17923927810272372 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1040 0.16467212829225503 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 974 0.1542217816890927 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 936 0.14820491546302952 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 908 0.14377143508593035 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 812 0.12857093093587602 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 755 0.1195456315967813 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 748 0.11843726150250651 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 679 0.10751189914465495 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 650 0.10292008018265938 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.166771697927981E-4 0.0 12-13 0.0 0.0 0.0 3.9584646224099764E-4 0.0 14-15 0.0 0.0 0.0 6.333543395855962E-4 0.0 16-17 0.0 0.0 0.0 7.916929244819953E-4 0.0 18-19 0.0 0.0 0.0 0.0011083700942747935 0.0 20-21 0.0 0.0 0.0 0.0021375708961013876 0.0 22-23 0.0 0.0 0.0 0.0046709882544437725 0.0 24-25 0.0 0.0 0.0 0.008471114291957349 0.0 26-27 0.0 0.0 0.0 0.014092134055779516 0.0 28-29 0.0 0.0 0.0 0.029609315375626624 0.0 30-31 0.0 0.0 0.0 0.05628936693066987 0.0 32-33 0.0 0.0 0.0 0.09080717843808486 0.0 34-35 0.0 0.0 0.0 0.12065400169105608 0.0 36-37 0.0 0.0 0.0 0.1496299627270971 0.0 38-39 0.0 0.0 0.0 0.21336124314789773 0.0 40-41 0.0 0.0 0.0 0.3551534459226231 0.0 42-43 0.0 0.0 0.0 0.5709689371364151 0.0 44-45 0.0 0.0 0.0 0.7326326323156385 0.0 46-47 0.0 0.0 0.0 1.1361585159241114 0.0 48-49 0.0 0.0 0.0 1.45061894552836 0.0 50-51 0.0 0.0 0.0 1.6425253104227957 0.0 52-53 0.0 0.0 0.0 1.9064757314450929 0.0 54-55 0.0 0.0 0.0 2.1108908445463443 0.0 56-57 0.0 0.0 0.0 2.6995145338987077 0.0 58-59 0.0 0.0 0.0 3.556047108895778 0.0 60-61 0.0 0.0 0.0 4.266195662156129 0.0 62-63 0.0 0.0 0.0 4.814601350944807 0.0 64-65 0.0 0.0 0.0 5.676517437828355 0.0 66-67 0.0 0.0 0.0 6.808242473375367 0.0 68-69 0.0 0.0 0.0 7.793662656478107 0.0 70-71 0.0 0.0 0.0 8.299871112391894 0.0 72-73 0.0 1.5833858489639906E-4 0.0 8.668404168738263 0.0 74-75 0.0 1.5833858489639906E-4 0.0 9.170416652152298 0.0 76-77 0.0 1.5833858489639906E-4 0.0 9.70758030141333 0.0 78-79 0.0 1.5833858489639906E-4 0.0 10.255590143739767 0.0 80-81 0.0 1.5833858489639906E-4 0.0 11.165403652554476 0.0 82-83 0.0 1.5833858489639906E-4 0.0 12.067854417171503 0.0 84-85 0.0 1.5833858489639906E-4 0.0 13.54539092213225 0.0 86-87 0.0 1.5833858489639906E-4 0.0 14.748368320882642 0.0 88-89 0.0 1.5833858489639906E-4 0.0 16.14333125381992 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 85 1.2914825E-10 50.289967 2 TACCTGG 785 0.0 49.613743 2 ACCTGGG 885 0.0 42.39763 3 TAACGCA 115 5.4569682E-11 41.297672 4 GTACACG 115 2.4883775E-9 37.188515 1 CTCACCG 65 1.3287704E-4 36.532555 8 ACACGGG 110 7.009294E-8 34.542606 3 GTATAAC 185 0.0 33.391487 1 GTACCTG 1230 0.0 32.065434 1 TACACGG 120 1.5050864E-7 31.664057 2 CCTGGGG 560 0.0 29.6827 4 ATAACGC 135 4.223075E-7 28.145824 3 TCCGAAC 50 0.0016552935 23.746159 76-77 CATGGGT 445 0.0 23.47935 4 GTATAGA 220 5.6057615E-8 21.59939 1 ACGCAGT 140 4.8536985E-4 20.353851 5 GAGTACT 2855 0.0 20.29605 12-13 GGACAGT 215 1.0788881E-6 19.880505 6 TAGCGTT 60 0.0047627552 19.792383 26-27 CTTACAC 240 1.4118814E-7 19.790035 3 >>END_MODULE