FastQCFastQC Report
Wed 25 May 2016
SRR1003816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982729
Sequences flagged as poor quality0
Sequence length121
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT163170.8229566420827052No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT138620.699137401026565No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC88560.44665710745139653No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT62470.3150707938402071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51640.26044910827450446No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41800.21082054077990486No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41520.20940834576989595No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40260.20305346822485576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38400.1936724585155107No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37940.1913524238562103No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34320.17309475979823769No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31730.16003195595565506No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28260.14253082493875865No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26730.13481418791978125No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT26560.13395678380656156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.12941758556010427No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25600.1291149723436738No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25380.12800539055009535No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22320.11257211651214058No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.1114120991824904No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21080.10631811003924391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG21950.049.8066252
TATAACG3750.046.031812
ACCTGGG24100.043.3934973
TAACGCA4650.037.071374
GTACCTG33050.034.5712621
ATAACGC5600.033.8607253
GTATAAC7400.032.7520941
TACACGG3800.030.2840862
GTACACG4300.029.5240361
TATCACG3550.027.554252
CCTGGGG21400.026.8507584
GAGTACG5400.025.647141
GTACATG81950.025.0681631
TCACGCA3900.025.0469384
ACACGGG4900.024.6259823
TACGCAG4900.024.6259824
TACATGG80350.024.061432
CATGGGG31550.023.3120774
ACATGGG80700.022.7848823
AGTACGC5200.022.1306782
Produced by FastQC (version 0.11.5)