##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003816_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1982729 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.585237316849657 31.0 31.0 33.0 27.0 34.0 2 30.499470679048926 31.0 31.0 34.0 26.0 34.0 3 30.56153009311913 31.0 31.0 34.0 26.0 34.0 4 34.081954719984424 37.0 35.0 37.0 30.0 37.0 5 34.04156543834281 37.0 35.0 37.0 30.0 37.0 6 34.13687548827903 37.0 35.0 37.0 30.0 37.0 7 34.14979354213309 37.0 35.0 37.0 30.0 37.0 8 34.12341979161045 37.0 35.0 37.0 30.0 37.0 9 35.55615921288285 39.0 35.0 39.0 30.0 39.0 10-11 35.38497696861245 38.0 35.0 39.0 29.5 39.0 12-13 35.238959030709694 38.0 35.0 39.0 29.0 39.0 14-15 36.183825928808226 39.0 36.0 41.0 27.0 41.0 16-17 36.2272342816391 39.0 36.0 41.0 29.0 41.0 18-19 36.18843977164806 39.0 36.0 41.0 28.0 41.0 20-21 36.074026505891624 39.0 35.5 41.0 27.0 41.0 22-23 35.974923955820486 39.0 35.0 40.5 27.0 41.0 24-25 35.78239966228365 39.0 35.0 40.5 27.0 41.0 26-27 35.58894483310628 39.0 35.0 40.0 27.0 41.0 28-29 35.392879208404175 38.0 34.0 40.0 26.5 41.0 30-31 35.14224082060635 38.0 34.0 40.0 25.5 41.0 32-33 34.869473084823994 38.0 34.0 40.0 25.0 41.0 34-35 34.839278842443925 38.0 34.0 40.0 25.0 41.0 36-37 34.687834293037525 38.0 34.0 40.0 24.0 41.0 38-39 34.68122522039069 38.0 34.0 40.0 24.0 41.0 40-41 34.75931859573346 38.0 34.0 40.0 24.0 41.0 42-43 34.611975968475775 38.0 34.0 40.0 24.0 41.0 44-45 34.3943900048872 38.0 33.0 40.0 23.0 41.0 46-47 34.11846349148068 38.0 33.0 40.0 21.0 41.0 48-49 33.84871583559831 38.0 33.0 40.0 18.5 41.0 50-51 33.06854870231888 37.5 32.0 39.5 16.0 40.5 52-53 33.20768748527912 37.5 32.5 39.5 18.0 40.5 54-55 33.52819850821771 38.0 33.0 40.0 18.0 41.0 56-57 33.35738015634007 38.0 33.0 40.0 15.5 41.0 58-59 33.09003676246224 38.0 32.0 40.0 13.0 41.0 60-61 32.80378105126823 37.0 32.0 40.0 12.5 41.0 62-63 32.5285132259628 37.0 31.0 40.0 10.0 41.0 64-65 32.234328796320625 36.0 31.0 39.5 8.0 41.0 66-67 31.875144308677584 36.0 31.0 39.0 8.0 41.0 68-69 31.504502380305127 35.0 30.5 39.0 7.0 40.5 70-71 31.09885642465511 35.0 30.0 38.5 3.5 40.0 72-73 30.72197889878042 35.0 30.0 37.5 2.0 40.0 74-75 30.350506297128856 35.0 29.5 37.0 2.0 39.0 76-77 29.920118937081163 34.5 29.0 36.5 2.0 39.0 78-79 29.37788194957556 34.0 29.0 36.0 2.0 39.0 80-81 28.98716440824742 34.0 28.5 36.0 2.0 37.5 82-83 28.649088201161128 34.0 28.0 35.0 2.0 37.0 84-85 28.41564555720928 34.0 27.0 35.0 2.0 37.0 86-87 28.180211970471 34.0 27.0 35.0 2.0 36.0 88-89 27.859831827748522 34.0 26.5 35.0 2.0 36.0 90-91 27.52307148379834 34.0 26.0 35.0 2.0 35.5 92-93 27.278519404315972 33.5 25.5 35.0 2.0 35.0 94-95 27.04380805445424 33.0 25.0 35.0 2.0 35.0 96-97 26.74923602771735 33.0 25.0 35.0 2.0 35.0 98-99 26.4925647932723 33.0 25.0 35.0 2.0 35.0 100-101 26.185324872940274 33.0 24.0 35.0 2.0 35.0 102-103 25.885139623216283 32.5 23.0 35.0 2.0 35.0 104-105 25.57574358371719 32.0 20.0 35.0 2.0 35.0 106-107 25.24149240768658 32.0 19.0 35.0 2.0 35.0 108-109 24.90378614525737 32.0 18.0 34.5 2.0 35.0 110-111 24.510995451219003 32.0 15.0 34.0 2.0 35.0 112-113 24.01709588148456 31.0 7.0 34.0 2.0 35.0 114-115 23.499411921649404 31.0 2.0 34.0 2.0 35.0 116-117 22.84237482782569 31.0 2.0 34.0 2.0 35.0 118-119 22.076765155500322 30.5 2.0 34.0 2.0 35.0 120-121 20.426418083358847 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4527023645485535 1101 2 0.28412669547357083 1101 3 0.5059909855385598 1101 4 0.6914879063007078 1101 5 0.774109074844354 1101 6 0.6524434447161553 1101 7 0.4211152229451329 1101 8 0.43364120945209095 1101 9 0.5734714717542815 1101 10-11 0.625560296505725 1101 12-13 0.8080724639752006 1101 14-15 0.9108120343368924 1101 16-17 0.7717581302990197 1101 18-19 0.5676040059184402 1101 20-21 0.6549149154128315 1101 22-23 0.6723033531577371 1101 24-25 0.6428499586458827 1101 26-27 0.7277891979093596 1101 28-29 0.7011330083612677 1101 30-31 0.8252665757330888 1101 32-33 0.7788481832954375 1101 34-35 0.7247056930508577 1101 36-37 0.7186258901166056 1101 38-39 0.5879562098949762 1101 40-41 0.7353916672569625 1101 42-43 0.716678351039981 1101 44-45 0.7227740394372404 1101 46-47 0.75271261217231 1101 48-49 0.7222674496395314 1101 50-51 0.476200513738128 1101 52-53 0.5005970644863851 1101 54-55 0.6231657790231893 1101 56-57 0.6488999915590128 1101 58-59 0.7412583099348069 1101 60-61 0.5790917028342619 1101 62-63 0.3604177757541862 1101 64-65 0.4314509530229458 1101 66-67 0.3691167927219574 1101 68-69 0.43773288341037997 1101 70-71 0.26666309401453603 1101 72-73 0.2739979202143168 1101 74-75 0.2096706340538681 1101 76-77 0.16703250896202348 1101 78-79 0.45742346382201404 1101 80-81 0.5373404994765814 1101 82-83 0.4660714342642933 1101 84-85 0.3494449280760641 1101 86-87 0.38058391659126656 1101 88-89 0.5556177425787716 1101 90-91 0.5168084993979853 1101 92-93 0.3878633339514259 1101 94-95 0.6557394768835394 1101 96-97 0.5291310617275577 1101 98-99 0.5127901842993516 1101 100-101 0.4621948317920861 1101 102-103 0.47163775056078094 1101 104-105 0.5975253169569577 1101 106-107 0.37369668504156195 1101 108-109 0.5082873096226557 1101 110-111 0.34510361734656314 1101 112-113 0.25387228657228533 1101 114-115 0.3736873528080693 1101 116-117 0.268072342418062 1101 118-119 -0.02876842863146223 1101 120-121 0.16431151927125853 1102 1 0.47414297757537227 1102 2 0.3281726724850671 1102 3 0.5335312154236185 1102 4 0.8142925040018554 1102 5 0.8136339790589133 1102 6 0.7628495749843509 1102 7 0.473329782332101 1102 8 0.5735186040881075 1102 9 0.6436017399535174 1102 10-11 0.6363419057011299 1102 12-13 0.6850609697223291 1102 14-15 0.7990878964058581 1102 16-17 0.7141336092262236 1102 18-19 0.7406155001713088 1102 20-21 0.7841026548764347 1102 22-23 0.5597133148435631 1102 24-25 0.7985281195654181 1102 26-27 0.7513294277944169 1102 28-29 0.8173628934187391 1102 30-31 0.7629830508288862 1102 32-33 0.6376297924908343 1102 34-35 0.6275945819397464 1102 36-37 0.7708021353273367 1102 38-39 0.6173431830750502 1102 40-41 0.5829931998240099 1102 42-43 0.7103718376300208 1102 44-45 0.7260077559123417 1102 46-47 0.8458926888006673 1102 48-49 0.7610797101759275 1102 50-51 0.3756564524354431 1102 52-53 0.5032407426473071 1102 54-55 0.5666063920500051 1102 56-57 0.6069229800647662 1102 58-59 0.5327525628083691 1102 60-61 0.6519269518764048 1102 62-63 0.5874062815013161 1102 64-65 0.7185927154750544 1102 66-67 0.6561282869748339 1102 68-69 0.46993977996210035 1102 70-71 0.43581251930189424 1102 72-73 0.45997493170856885 1102 74-75 0.2292415152799201 1102 76-77 0.2120823173911397 1102 78-79 0.3198602454312116 1102 80-81 0.31736348798232683 1102 82-83 0.45111357985815914 1102 84-85 0.434464085164187 1102 86-87 0.6515417709973939 1102 88-89 0.7193188920040576 1102 90-91 0.8066782311987488 1102 92-93 0.6651353646027651 1102 94-95 0.5677164883777905 1102 96-97 0.5008168854823474 1102 98-99 0.9092039774028002 1102 100-101 0.6177733758533925 1102 102-103 0.5945649536259126 1102 104-105 0.6625981138918213 1102 106-107 0.5639112444285352 1102 108-109 0.5334750490862596 1102 110-111 0.37947143343851764 1102 112-113 0.4092439340818679 1102 114-115 0.4696490386318217 1102 116-117 0.4314746412686361 1102 118-119 0.4083580081501488 1102 120-121 0.6382885307655108 1103 1 0.6410729083945057 1103 2 0.6514147516796598 1103 3 0.6689792759366853 1103 4 0.9867665948252977 1103 5 0.9391124576182293 1103 6 0.8613448238470411 1103 7 0.8446291443606881 1103 8 0.8194137829623713 1103 9 1.017226871181812 1103 10-11 0.9713323495892539 1103 12-13 0.9887703562468602 1103 14-15 1.0447074285601516 1103 16-17 0.8346100688886651 1103 18-19 0.7793885492439685 1103 20-21 0.9107967785405933 1103 22-23 0.8368680161814126 1103 24-25 0.8748031202383828 1103 26-27 1.005325497675173 1103 28-29 0.9848925712027992 1103 30-31 1.0016058939740518 1103 32-33 0.9405203144429777 1103 34-35 0.9009903664830716 1103 36-37 0.9835007274832535 1103 38-39 0.8340009665954682 1103 40-41 0.8692329370982321 1103 42-43 0.900722587313723 1103 44-45 0.9792226472956926 1103 46-47 0.9753459715269841 1103 48-49 0.8925120502119981 1103 50-51 0.8010805553722733 1103 52-53 0.8767797342756154 1103 54-55 0.936455916936275 1103 56-57 0.8207063928080345 1103 58-59 0.9609772794898461 1103 60-61 0.8033878256549656 1103 62-63 0.9769548561601198 1103 64-65 0.8981883977276084 1103 66-67 1.1274384164534155 1103 68-69 1.0135845617866472 1103 70-71 0.8383789410090721 1103 72-73 0.8532953441019053 1103 74-75 0.7935280371243856 1103 76-77 0.6960915773981426 1103 78-79 0.8525491468821258 1103 80-81 0.8894294924508195 1103 82-83 0.9877649029085802 1103 84-85 1.0619065466032573 1103 86-87 1.0784501669165323 1103 88-89 1.076184487309451 1103 90-91 1.1433107112116723 1103 92-93 1.0298003623146847 1103 94-95 1.0880938875002464 1103 96-97 1.0556285897004969 1103 98-99 1.0597878423789773 1103 100-101 0.8499955083468613 1103 102-103 0.8554295798608038 1103 104-105 1.0640129568216459 1103 106-107 1.002298818791239 1103 108-109 1.0741582437886734 1103 110-111 1.1954158733965237 1103 112-113 1.151997709419021 1103 114-115 1.3132340611088757 1103 116-117 1.0188045828552212 1103 118-119 0.8782026876839168 1103 120-121 1.103287319427384 1104 1 0.4420254477793861 1104 2 0.6143333375399926 1104 3 0.6249319449481519 1104 4 0.7153144745663909 1104 5 0.8842091158447616 1104 6 0.671717327454985 1104 7 0.5737687694805942 1104 8 0.7156530995709929 1104 9 0.6313419104586657 1104 10-11 0.8220479963827287 1104 12-13 0.882875047001022 1104 14-15 0.9118390946074939 1104 16-17 0.8390531282489988 1104 18-19 0.8638161830402069 1104 20-21 0.8248942102057839 1104 22-23 0.7699482896071004 1104 24-25 0.7925252353986565 1104 26-27 0.9305899359167427 1104 28-29 0.9278648656798438 1104 30-31 0.9589149972173132 1104 32-33 0.9888832386459825 1104 34-35 0.8560355413493355 1104 36-37 0.9862804616623251 1104 38-39 0.6708539138720155 1104 40-41 0.858222130561586 1104 42-43 0.7401145954024315 1104 44-45 0.973255179248639 1104 46-47 1.1161207712539039 1104 48-49 1.222308450049539 1104 50-51 1.0376025227582204 1104 52-53 1.0889826733424783 1104 54-55 1.0000116174815687 1104 56-57 1.0269528820879188 1104 58-59 1.1214612619643276 1104 60-61 1.0503135150523804 1104 62-63 1.0218193747701747 1104 64-65 0.8920827693411155 1104 66-67 0.8617121186752144 1104 68-69 0.9276227973095139 1104 70-71 0.930471827101865 1104 72-73 0.9050126803619207 1104 74-75 1.0445351276988042 1104 76-77 1.0232448910858452 1104 78-79 1.133715591743293 1104 80-81 1.1078331194027768 1104 82-83 1.0841019795697449 1104 84-85 1.0341138497652835 1104 86-87 0.930988795640058 1104 88-89 0.9601325027263705 1104 90-91 1.2076465477784701 1104 92-93 1.152017495493041 1104 94-95 1.1309953194419649 1104 96-97 1.032022615643097 1104 98-99 1.053939630793817 1104 100-101 1.0638303381471523 1104 102-103 1.1612753069363428 1104 104-105 1.168584562549409 1104 106-107 1.0991534296090109 1104 108-109 1.143605472804289 1104 110-111 1.0560661019714104 1104 112-113 0.9077039798892201 1104 114-115 0.7945899768343097 1104 116-117 0.6432556142507799 1104 118-119 0.5469572786256087 1104 120-121 0.561275233908404 1105 1 0.8647505309415457 1105 2 0.9656439368528815 1105 3 0.9145821185434855 1105 4 1.1026997289608005 1105 5 1.1569837327534955 1105 6 1.0557888196477379 1105 7 0.9654480089330875 1105 8 1.0499718060585508 1105 9 1.159857897588985 1105 10-11 1.0894781946994883 1105 12-13 1.2179837940244624 1105 14-15 1.4321256632367252 1105 16-17 1.3232961708025783 1105 18-19 1.114638488677059 1105 20-21 1.4023456762229003 1105 22-23 1.2634407368468459 1105 24-25 1.3894805005178057 1105 26-27 1.463808902342862 1105 28-29 1.5354417111527283 1105 30-31 1.2660208176597507 1105 32-33 1.3555772473348426 1105 34-35 1.2436536952402335 1105 36-37 1.3904901484636198 1105 38-39 1.3775566477712715 1105 40-41 1.4891836760144912 1105 42-43 1.335314366365651 1105 44-45 1.4024116967990423 1105 46-47 1.6253015557957369 1105 48-49 1.5971716641989175 1105 50-51 1.3999257463599122 1105 52-53 1.3408433699051017 1105 54-55 1.2811219913931922 1105 56-57 1.4349032756312639 1105 58-59 1.518007078407713 1105 60-61 1.4853752277384729 1105 62-63 1.539031897264863 1105 64-65 1.5980672639151194 1105 66-67 1.326243229503568 1105 68-69 1.3288231953972378 1105 70-71 1.1263543912230745 1105 72-73 1.0261654078990468 1105 74-75 1.0519508748858364 1105 76-77 1.2080757492466425 1105 78-79 1.2966418546266105 1105 80-81 1.3136360659790398 1105 82-83 1.2388147292834475 1105 84-85 1.3303754152134495 1105 86-87 1.3301460402913214 1105 88-89 1.2928000085886246 1105 90-91 1.304313699179044 1105 92-93 1.2535425895617145 1105 94-95 1.2462058151429822 1105 96-97 1.3063998083066473 1105 98-99 1.3499921547427007 1105 100-101 1.4111559702704675 1105 102-103 1.62257808991491 1105 104-105 1.6705784094583223 1105 106-107 1.732154265775005 1105 108-109 1.64051938406163 1105 110-111 1.44888030043359 1105 112-113 1.2248596976286628 1105 114-115 1.2763649663822356 1105 116-117 1.375596151941874 1105 118-119 1.2139573513356972 1105 120-121 1.19139197904137 1106 1 0.5666456946981846 1106 2 0.7741477078899983 1106 3 0.7609632873532668 1106 4 0.8052644105987028 1106 5 0.885919326356607 1106 6 0.8584593632134485 1106 7 0.8003791516302599 1106 8 0.7910076532597969 1106 9 0.8486144198599774 1106 10-11 0.9453864665152807 1106 12-13 1.0236292930105364 1106 14-15 0.9724070677657366 1106 16-17 0.9830220589841545 1106 18-19 0.8806431654217803 1106 20-21 1.0517942532033473 1106 22-23 0.6975863840396244 1106 24-25 1.0002845336220076 1106 26-27 0.984176972503441 1106 28-29 1.0089668192495154 1106 30-31 0.8847743757967663 1106 32-33 0.8835988996278132 1106 34-35 1.0285401406790058 1106 36-37 0.8891824007819977 1106 38-39 0.8912831758293933 1106 40-41 0.959197461589973 1106 42-43 0.9719741167197 1106 44-45 0.9332751537320476 1106 46-47 0.887147505518378 1106 48-49 0.84727540888818 1106 50-51 0.703601978239881 1106 52-53 0.6827651638178125 1106 54-55 0.8319171320954553 1106 56-57 0.6940843277576789 1106 58-59 0.8648208696291704 1106 60-61 0.7177863860561615 1106 62-63 0.8422783297178924 1106 64-65 0.8060017330293192 1106 66-67 0.8069104889970333 1106 68-69 0.6714883752919434 1106 70-71 0.5605656728110979 1106 72-73 0.6222882736049584 1106 74-75 0.678762642968227 1106 76-77 0.870674024402966 1106 78-79 0.9072534542709185 1106 80-81 0.8478104135166973 1106 82-83 1.0393812082216307 1106 84-85 0.9608521232368403 1106 86-87 0.845165260112438 1106 88-89 0.7649797292072336 1106 90-91 0.8797266783218944 1106 92-93 0.9367547697998795 1106 94-95 0.852971568574084 1106 96-97 1.1213978557422024 1106 98-99 1.1575829254645846 1106 100-101 0.9079337684364681 1106 102-103 0.9515626152535503 1106 104-105 1.005705514346321 1106 106-107 0.9487734121985234 1106 108-109 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28155.0 3 4518.0 4 4203.0 5 4723.0 6 5770.0 7 6160.0 8 7696.0 9 9658.0 10 12653.0 11 15133.0 12 15465.0 13 17316.0 14 20442.0 15 20159.0 16 15488.0 17 15173.0 18 16029.0 19 17094.0 20 18554.0 21 20956.0 22 23164.0 23 25822.0 24 29146.0 25 33040.0 26 37614.0 27 43545.0 28 50955.0 29 59086.0 30 69537.0 31 82966.0 32 100227.0 33 123244.0 34 156555.0 35 204059.0 36 271233.0 37 296853.0 38 99896.0 39 442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.56542396345824 18.859154165904453 13.351437332175273 22.223984538462048 2 14.714066031811942 21.023990750946226 40.11863580527729 24.14330741196454 3 19.78709893422262 26.49677458090922 30.153165429043323 23.562961055824836 4 11.793025109585145 16.344299563083755 40.34849526127225 31.514180066058845 5 11.611422629349596 37.9982214462247 35.782914691485026 14.607441232940687 6 27.87950975982546 37.502111525162256 19.107420153820794 15.51095856119149 7 26.087706268948256 32.55376726961971 23.89286222963762 17.465664231794413 8 24.03555850327957 33.5304397714881 21.335763171172346 21.098238554059993 9 24.216550298650784 18.337727650017086 23.578306316423355 33.86741573490878 10-11 23.78529214473409 25.84884991738446 29.060710346191932 21.305147591689522 12-13 25.81533686435129 23.89295873926944 28.768006322571686 21.52369807380758 14-15 22.22157520992441 25.6978197154762 28.452557922151367 23.62804715244802 16-17 20.280500770690367 28.940720430162227 29.08236975841528 21.69640904073213 18-19 19.883666731799458 29.23854171755166 30.448764919479586 20.429026631169297 20-21 21.18213241659821 27.459034014024258 29.45859593091516 21.900237638462375 22-23 20.511114468418672 27.200320877213596 29.83540036655694 22.453164287810786 24-25 21.466938580984664 27.277059887017717 29.8136552766306 21.442346255367013 26-27 21.163779225581536 26.410002222738772 31.263939801927016 21.162278749752677 28-29 20.232858365692437 28.13600108874132 30.695458956614424 20.93568158895182 30-31 20.685433956043454 27.597266974689138 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22.181412518349102 56-57 21.343148334809655 27.778821385299896 28.760703340562227 22.117326939328223 58-59 22.090351577587825 27.35302334741435 28.68000775550022 21.8766173194976 60-61 21.50500501965235 27.806023837304732 29.85712122619482 20.831849916848093 62-63 20.862360528899394 28.125861021056807 29.480928486946063 21.530849963097733 64-65 20.385180602535023 29.213544026327554 29.0434894536911 21.35778591744633 66-67 20.68251121920395 28.08890295379044 28.590634963315793 22.63795086368982 68-69 20.452437661902472 28.69060035046375 28.71523000869417 22.141731978939607 70-71 21.38968141598325 28.102756297833658 28.353051226485864 22.154511059697224 72-73 21.001874758402504 27.39597915600499 29.70933483103635 21.892811254556158 74-75 20.32625121328153 28.919338346109587 29.230656080557317 21.52375436005157 76-77 20.884855022351342 27.95295922696913 28.902830779615496 22.25935497106403 78-79 20.85698512598049 28.20206991593831 29.078463686837875 21.862481271243325 80-81 21.356266693020604 28.13355027786254 28.674553150475724 21.835629878641136 82-83 20.881659781802774 27.663479870939355 29.695074877987178 21.759785469270692 84-85 20.73459109508346 28.18215529837673 28.99922449308903 22.084029113450782 86-87 21.12775621858897 28.475959030472676 28.54520007634073 21.851084674597622 88-89 20.68250211077618 27.74809317645478 28.41135274416171 23.158051968607328 90-91 20.74452111133281 26.813469392741236 29.01716453768441 23.424844958241543 92-93 21.34333994435343 27.38873357996153 29.068131167931615 22.19979530775342 94-95 20.575981427406788 27.07692339008858 29.53231120289296 22.814783979611672 96-97 19.454105999902282 27.54347445481344 30.205177847266327 22.79724169801795 98-99 20.345114978079653 28.33709321607183 28.7677868927993 22.55000491304921 100-101 19.744482035754395 27.769876023339418 28.982866032512693 23.502775908393495 102-103 20.23844815560583 27.197049641116518 29.33584608177408 23.22865612150357 104-105 21.09287239983869 27.170217935849433 29.23917731156901 22.497732352742865 106-107 20.662648947040598 27.300155511498485 29.66089231846475 22.37630322299616 108-109 20.462266378443513 27.009038523385563 29.548190870490913 22.980504227680008 110-111 20.39923865855933 27.634781020198545 28.623222236950603 23.34275808429152 112-113 21.070662100630912 26.739376154931723 28.154226356693297 24.035735387744072 114-115 20.73040860120928 26.725309578950235 28.450727306640864 24.093554513199617 116-117 21.435747584751642 26.70081719455747 27.933697916732953 23.92973730395794 118-119 21.470988366839702 26.71233730947057 28.218654035335994 23.598020288353734 120-121 21.655989526011297 27.62527244203489 27.46882423369515 23.24991379825866 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4661.0 1 5211.5 2 3856.5 3 1787.5 4 1451.0 5 1287.0 6 1321.5 7 1642.5 8 2072.0 9 2150.5 10 1998.5 11 1889.5 12 1952.5 13 2102.5 14 2281.0 15 2537.0 16 2702.5 17 2893.5 18 3231.5 19 3563.0 20 3917.0 21 4417.0 22 5124.5 23 6183.0 24 7468.5 25 8963.0 26 11078.5 27 13788.0 28 17280.0 29 21608.5 30 25855.0 31 29981.5 32 34304.5 33 38393.5 34 42311.5 35 46992.5 36 52113.5 37 57884.0 38 63451.5 39 66729.0 40 69296.0 41 71824.0 42 73985.0 43 76978.5 44 78543.5 45 79835.0 46 83083.5 47 94280.5 48 123977.5 49 122456.0 50 90963.0 51 73520.5 52 59882.0 53 50515.5 54 43945.5 55 38010.0 56 31873.5 57 25564.0 58 20719.0 59 17211.5 60 14020.5 61 10968.0 62 8292.0 63 5848.0 64 3867.5 65 2573.5 66 1667.5 67 975.5 68 576.5 69 381.5 70 247.0 71 162.5 72 113.5 73 93.5 74 78.5 75 55.5 76 42.5 77 35.5 78 31.0 79 27.5 80 19.5 81 13.5 82 12.0 83 8.0 84 8.0 85 5.5 86 4.0 87 3.5 88 2.0 89 4.0 90 5.0 91 3.5 92 2.0 93 1.5 94 1.5 95 0.5 96 1.0 97 1.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46985745404440044 2 0.8853958357395287 3 0.8777296342566231 4 0.8526631728289645 5 0.8617920048579508 6 0.8737956624430268 7 0.849788347272875 8 0.8380873029042295 9 0.8348594285956376 10-11 0.8267393072880862 12-13 0.829185430787566 14-15 0.8284541155145257 16-17 0.8553362562407671 18-19 0.8832775432245152 20-21 0.8749808975407127 22-23 0.8507466224582381 24-25 0.8406847330119245 26-27 0.8415673548931801 28-29 0.8652972746149373 30-31 0.8719295476083722 32-33 0.8646163948779687 34-35 0.8788896515862732 36-37 0.8880941369193672 38-39 0.8745774132521389 40-41 0.884740173770596 42-43 0.8774270210401925 44-45 0.8587154371575743 46-47 0.8534449236380767 48-49 0.8585136950132872 50-51 0.9064526720494834 52-53 0.862573755667063 54-55 0.8762670037105423 56-57 0.9274590728233661 58-59 0.8912967934599232 60-61 0.9053935257919766 62-63 0.8777044164885872 64-65 0.8666590340888745 66-67 0.8693825530367488 68-69 0.8885984922800847 70-71 0.9001230122724789 72-73 0.9049396059673309 74-75 0.9086466178686043 76-77 0.9232477055613753 78-79 0.9668996620314727 80-81 0.9939835449020014 82-83 0.9583256208992758 84-85 0.918305023026344 86-87 0.9004760610249813 88-89 0.8983577685099677 90-91 0.9127318962904158 92-93 0.8991647370871159 94-95 0.8918515843567124 96-97 0.9026447890760663 98-99 0.936688775924496 100-101 0.9243320695869177 102-103 0.8985342928862189 104-105 0.8869845551257888 106-107 0.887715870398829 108-109 0.8869089018216811 110-111 0.8833027609925511 112-113 0.8970464445721024 114-115 0.8998960523601561 116-117 0.9273329839831868 118-119 0.9230459634170882 120-121 0.9008543275455194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1982729.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.774558074535875 #Duplication Level Percentage of deduplicated Percentage of total 1 80.78950191626056 47.483672721900845 2 11.970039360717703 14.070675471219657 3 3.097203479595985 5.4611029734050645 4 1.286384515882959 3.0242672573978675 5 0.6932193075563914 2.0371829225181335 6 0.42394316021466505 1.4950243134203458 7 0.2905907243552298 1.1955538983176532 8 0.21296221224788242 1.0013407929155842 9 0.1735152478632809 0.9178453811122101 >10 0.9583613747607226 10.395184391561717 >50 0.0697426880681296 2.7993762700387945 >100 0.029056843998672956 2.9920212010518177 >500 0.002087302277270775 0.8560015763513572 >1k 0.0029570115594669292 3.69211380976246 >5k 2.609127846588467E-4 1.0359837542047623 >10k+ 1.7394185643923113E-4 1.5426532648219038 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16317 0.8229566420827052 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13862 0.699137401026565 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8856 0.44665710745139653 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6247 0.3150707938402071 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5164 0.26044910827450446 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4180 0.21082054077990486 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4152 0.20940834576989595 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4026 0.20305346822485576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3840 0.1936724585155107 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3794 0.1913524238562103 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3432 0.17309475979823769 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3173 0.16003195595565506 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2826 0.14253082493875865 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2673 0.13481418791978125 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2656 0.13395678380656156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2566 0.12941758556010427 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2560 0.1291149723436738 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2538 0.12800539055009535 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2232 0.11257211651214058 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2209 0.1114120991824904 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2108 0.10631811003924391 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.7652437625111653E-4 0.0 16-17 0.0 0.0 0.0 2.521776803587379E-4 0.0 18-19 0.0 2.5217768035873787E-5 0.0 3.5304875250223306E-4 0.0 20-21 0.0 5.0435536071747574E-5 0.0 4.7913759268160196E-4 0.0 22-23 0.0 5.0435536071747574E-5 0.0 0.0010087107214349516 0.0 24-25 0.0 5.0435536071747574E-5 0.0 0.0022948168912645148 0.0 26-27 5.0435536071747574E-5 5.0435536071747574E-5 0.0 0.0043122383341344175 0.0 28-29 5.0435536071747574E-5 5.0435536071747574E-5 0.0 0.01071755141524636 0.0 30-31 5.0435536071747574E-5 5.0435536071747574E-5 0.0 0.025116896963730293 0.0 32-33 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.04347543209384641 0.0 34-35 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.05822782639483258 0.0 36-37 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.07883074288014147 0.0 38-39 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.1121686322235666 0.0 40-41 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.15322315858596913 0.0 42-43 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.19069676188727758 0.0 44-45 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.20582742270880186 0.0 46-47 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.22645555696214664 0.0 48-49 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.2547246749303611 0.0 50-51 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.2864234093514545 0.0 52-53 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.35504095617706705 0.0 54-55 5.0435536071747574E-5 1.0087107214349515E-4 0.0 0.3897658227624653 0.0 56-57 5.0435536071747574E-5 1.5130660821524274E-4 0.0 0.4947978266318796 0.0 58-59 5.0435536071747574E-5 1.5130660821524274E-4 0.0 0.7200177129602684 0.0 60-61 5.0435536071747574E-5 1.5130660821524274E-4 0.0 0.8815122994620042 0.0 62-63 5.0435536071747574E-5 1.5130660821524274E-4 0.0 0.9515168235295898 0.0 64-65 5.0435536071747574E-5 1.5130660821524274E-4 0.0 1.1200219495452983 0.0 66-67 5.0435536071747574E-5 1.5130660821524274E-4 0.0 1.4991963097326968 0.0 68-69 5.0435536071747574E-5 1.5130660821524274E-4 0.0 1.9098676621969013 0.0 70-71 5.0435536071747574E-5 1.5130660821524274E-4 0.0 2.18917966096224 0.0 72-73 5.0435536071747574E-5 1.5130660821524274E-4 0.0 2.4881615187955592 0.0 74-75 5.0435536071747574E-5 1.5130660821524274E-4 0.0 2.8329640611500615 0.0 76-77 5.0435536071747574E-5 1.5130660821524274E-4 0.0 2.9705522035537886 0.0 78-79 5.0435536071747574E-5 1.5130660821524274E-4 0.0 3.1715125970316667 0.0 80-81 5.0435536071747574E-5 1.5130660821524274E-4 0.0 3.4571290378059736 0.0 82-83 5.0435536071747574E-5 1.5130660821524274E-4 0.0 3.7022205253466307 0.0 84-85 5.0435536071747574E-5 1.5130660821524274E-4 0.0 4.175331071467658 0.0 86-87 5.0435536071747574E-5 1.7652437625111653E-4 0.0 5.081481130300713 0.0 88-89 5.0435536071747574E-5 2.017421442869903E-4 0.0 6.147385749641025 0.0 90-91 5.0435536071747574E-5 2.017421442869903E-4 0.0 7.238407265945069 0.0 92-93 5.0435536071747574E-5 3.026132164304855E-4 0.0 8.227977701440793 0.0 94-95 5.0435536071747574E-5 3.026132164304855E-4 0.0 9.144467045168554 0.0 96-97 5.0435536071747574E-5 3.026132164304855E-4 0.0 10.17269631906327 0.0 98-99 5.0435536071747574E-5 3.026132164304855E-4 0.0 11.212979686079137 0.0 100-101 5.0435536071747574E-5 3.026132164304855E-4 0.0 12.018813463665484 0.0 102-103 5.0435536071747574E-5 3.026132164304855E-4 0.0 12.8978039863239 0.0 104-105 5.0435536071747574E-5 3.026132164304855E-4 0.0 13.873681173776145 0.0 106-107 5.0435536071747574E-5 3.026132164304855E-4 0.0 14.883224081556278 0.0 108-109 5.0435536071747574E-5 3.026132164304855E-4 0.0 15.874358018670225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2195 0.0 49.806625 2 TATAACG 375 0.0 46.03181 2 ACCTGGG 2410 0.0 43.393497 3 TAACGCA 465 0.0 37.07137 4 GTACCTG 3305 0.0 34.571262 1 ATAACGC 560 0.0 33.860725 3 GTATAAC 740 0.0 32.752094 1 TACACGG 380 0.0 30.284086 2 GTACACG 430 0.0 29.524036 1 TATCACG 355 0.0 27.55425 2 CCTGGGG 2140 0.0 26.850758 4 GAGTACG 540 0.0 25.64714 1 GTACATG 8195 0.0 25.068163 1 TCACGCA 390 0.0 25.046938 4 ACACGGG 490 0.0 24.625982 3 TACGCAG 490 0.0 24.625982 4 TACATGG 8035 0.0 24.06143 2 CATGGGG 3155 0.0 23.312077 4 ACATGGG 8070 0.0 22.784882 3 AGTACGC 520 0.0 22.130678 2 >>END_MODULE