FastQCFastQC Report
Wed 25 May 2016
SRR1003816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982729
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT213231.0754369356578735No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT103320.521099958693296No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC74110.37377775782772127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71220.35920188790298624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57380.2893991059796876No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54720.2759832533846028No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53040.2675100833245491No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA50410.2542455373376796No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46840.23624005096006565No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC43760.22070590584996738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.19659771960767206No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC38980.19659771960767206No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37710.1901924065265601No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG35500.1790461530547039No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA32770.1652772517071168No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29330.14792742729843567No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29230.14742307193771817No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.14232908279447168No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26630.1343098325590638No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC21380.10783117612139631No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20060.10117368535992564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3550.052.204612
TACCTGG21800.051.4432142
ACCTGGG25650.042.6079983
TAACGCA4650.040.8543784
ATAACGC5400.037.8377573
GTACCTG30500.037.5757561
GTATAAC6550.034.122991
GTACACG3300.025.9387531
GCGTTAG1109.406567E-525.9387531
CCTGGGG20850.024.6008034
TACACGG3000.023.7597892
TACGCAG4000.021.3719464
CTGGGGG14100.020.5467115
AGTACTT83400.020.21695712-13
AACCGTA1200.004770024719.7878447
GAGTACT81300.019.68760312-13
CCGGTCG3153.2196112E-1019.6013649
TCACGCA3404.1836756E-1119.556034
CATGGGG28600.018.7648014
CCCGGTC3801.0913936E-1118.7463768
Produced by FastQC (version 0.11.5)