##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1003816_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1982729
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58564786211328	31.0	31.0	34.0	30.0	34.0
2	31.806177243586994	31.0	31.0	34.0	30.0	34.0
3	31.83472880055721	31.0	31.0	34.0	30.0	34.0
4	35.607818315059696	37.0	35.0	37.0	33.0	37.0
5	35.475036174888245	37.0	35.0	37.0	33.0	37.0
6	35.50373298620235	37.0	35.0	37.0	33.0	37.0
7	35.42879737977303	37.0	35.0	37.0	33.0	37.0
8	35.42392076779025	37.0	35.0	37.0	33.0	37.0
9	36.98965718461777	39.0	37.0	39.0	33.0	39.0
10-11	36.90673687629525	39.0	37.0	39.0	33.0	39.0
12-13	36.84715964713281	39.0	37.0	39.0	32.0	39.0
14-15	37.9311678499684	40.0	37.0	41.0	33.0	41.0
16-17	37.939737856257715	40.0	37.0	41.0	33.0	41.0
18-19	37.95803208607934	40.0	37.0	41.0	33.0	41.0
20-21	37.88802630112335	40.0	37.0	41.0	32.5	41.0
22-23	37.79065671607164	39.5	37.0	41.0	32.0	41.0
24-25	37.694218927548846	39.0	37.0	41.0	32.0	41.0
26-27	37.45633240851372	39.0	36.0	41.0	32.0	41.0
28-29	37.269124524834204	39.0	36.0	41.0	31.5	41.0
30-31	37.031601393836475	39.0	36.0	40.0	31.0	41.0
32-33	36.76486347856918	39.0	35.0	40.0	30.5	41.0
34-35	36.70698617915005	39.0	35.0	40.0	30.0	41.0
36-37	36.49531176474446	38.5	35.0	40.0	30.0	41.0
38-39	36.23374550934596	38.0	35.0	40.0	30.0	41.0
40-41	36.34474277624426	39.0	35.0	40.0	30.0	41.0
42-43	36.20112733510227	39.0	35.0	40.0	30.0	41.0
44-45	36.03200739990185	39.0	35.0	40.0	29.5	41.0
46-47	35.742987064798065	39.0	35.0	40.0	28.5	41.0
48-49	35.48360012891323	38.5	35.0	40.0	27.0	41.0
50-51	35.22719090707807	38.0	34.0	40.0	26.5	41.0
52-53	35.025134801579036	38.0	34.0	40.0	26.0	41.0
54-55	34.80508052285512	38.0	34.0	40.0	26.0	41.0
56-57	34.551907497192005	38.0	34.0	40.0	25.0	41.0
58-59	34.27762972145966	38.0	33.0	40.0	24.0	41.0
60-61	33.84807353904644	37.0	33.0	40.0	23.0	41.0
62-63	33.59970601126024	37.0	33.0	40.0	22.5	41.0
64-65	33.15545921807771	36.0	32.0	39.0	22.0	41.0
66-67	32.7935126283017	36.0	32.0	39.0	21.0	40.5
68-69	32.526484456524315	35.5	31.0	39.0	20.5	40.0
70-71	32.112104074737395	35.0	31.0	38.5	19.5	40.0
72-73	31.73289667927387	35.0	31.0	37.5	18.5	40.0
74-75	31.350539080227303	35.0	31.0	37.0	18.0	39.0
76-77	30.363255139759392	34.0	29.5	36.0	17.0	39.0
78-79	30.744624454476636	34.0	30.0	36.0	16.0	39.0
80-81	30.558170582061393	34.0	30.0	36.0	13.0	37.5
82-83	30.246833026601216	34.0	30.0	35.0	9.5	37.0
84-85	29.898545893059513	34.0	30.0	35.0	7.0	37.0
86-87	29.54820351142289	34.0	29.5	35.0	4.5	36.0
88-89	29.22248401067418	34.0	29.0	35.0	2.0	36.0
90-91	28.925717029407448	34.0	29.0	35.0	2.0	36.0
92-93	28.670865256926184	34.0	29.0	35.0	2.0	35.0
94-95	28.341264489499068	34.0	29.0	35.0	2.0	35.0
96-97	27.959373419161167	34.0	29.0	35.0	2.0	35.0
98-99	27.563934607301352	34.0	28.0	35.0	2.0	35.0
100-101	26.58224749827132	33.0	25.5	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.040641415890952715
1101	2	0.03861184506510895
1101	3	0.09990976751769765
1101	4	0.09702528402062427
1101	5	0.07943423984974629
1101	6	0.04538730047089956
1101	7	0.059474790228073005
1101	8	0.08149318814073325
1101	9	0.10195170948288279
1101	10-11	0.16882058840114667
1101	12-13	0.12046944129718185
1101	14-15	0.1211673724945257
1101	16-17	0.1246613460360777
1101	18-19	0.018989412611375656
1101	20-21	-0.006413692231504342
1101	22-23	-0.14156917712756467
1101	24-25	0.022184902296089604
1101	26-27	0.03445680359030945
1101	28-29	-0.06051916811345137
1101	30-31	0.10749236253901273
1101	32-33	0.11866686014625571
1101	34-35	0.1188432392978882
1101	36-37	0.15446364890136977
1101	38-39	0.10077280213713635
1101	40-41	0.18251751704946173
1101	42-43	0.020753902100686616
1101	44-45	-0.002334127179921097
1101	46-47	-0.07256983954236063
1101	48-49	-0.12994016493051674
1101	50-51	-0.22362201112201063
1101	52-53	-0.07306359421126274
1101	54-55	0.10083558982434937
1101	56-57	-0.044434181914212445
1101	58-59	-0.20350077685042578
1101	60-61	-7.989117812030599E-4
1101	62-63	-0.13816220262517476
1101	64-65	-0.28072394680474844
1101	66-67	-0.40083765387390713
1101	68-69	-0.4038755240727525
1101	70-71	-0.342029916050123
1101	72-73	-0.3979009267964848
1101	74-75	-0.5937256903013015
1101	76-77	-0.44215898479664517
1101	78-79	-0.21694339907191917
1101	80-81	-0.28043581537477635
1101	82-83	-0.27889919358580784
1101	84-85	-0.4341273115505011
1101	86-87	-0.5234955816853386
1101	88-89	-0.28920896161052667
1101	90-91	-0.34454536708790684
1101	92-93	-0.1888034793398674
1101	94-95	-0.3481147375604756
1101	96-97	-0.31778274341293766
1101	98-99	-0.438505163006365
1101	100-101	-0.3990114427836424
1102	1	-0.0345923005841442
1102	2	-0.051993518919566384
1102	3	-0.07268793363172676
1102	4	-0.023066670002357625
1102	5	-0.09316346129967457
1102	6	-0.001877067345191108
1102	7	-0.15917655076809467
1102	8	-0.08468305706999502
1102	9	-0.05725135565121775
1102	10-11	-0.13862002462567347
1102	12-13	-0.06867232115492783
1102	14-15	0.009244000847019151
1102	16-17	0.04396402802841948
1102	18-19	-0.07307955290586676
1102	20-21	-0.1065516232659931
1102	22-23	-0.12294848747239229
1102	24-25	-0.015799772033410875
1102	26-27	-0.09698380943650875
1102	28-29	-0.06099426389889118
1102	30-31	-0.16791759914681137
1102	32-33	-0.292091760543407
1102	34-35	-0.3164211285182006
1102	36-37	-0.3523179602940303
1102	38-39	-0.24086704460615493
1102	40-41	-0.09044800019191968
1102	42-43	-0.12416563812919179
1102	44-45	-0.029621483501763635
1102	46-47	-0.1709223299638083
1102	48-49	-0.25087503083089757
1102	50-51	-0.19323886935955414
1102	52-53	-0.19402144861739146
1102	54-55	-0.25678126841319227
1102	56-57	-0.15480966084140846
1102	58-59	-0.27741648566269106
1102	60-61	-0.28155753247087034
1102	62-63	-0.3681851911309266
1102	64-65	-0.388877203509729
1102	66-67	-0.40794110214977053
1102	68-69	-0.43426632867045356
1102	70-71	-0.2977923681574026
1102	72-73	-0.26518828311832365
1102	74-75	-0.3710436979641365
1102	76-77	-0.3673084100840036
1102	78-79	-0.37891274773092576
1102	80-81	-0.16822125598780246
1102	82-83	-0.2603168181068831
1102	84-85	-0.29770585561180596
1102	86-87	-0.5164764245972151
1102	88-89	-0.391392869656503
1102	90-91	-0.300070271302463
1102	92-93	-0.3423283835544275
1102	94-95	-0.3995476877520474
1102	96-97	-0.29546090433247585
1102	98-99	-0.2585894541941052
1102	100-101	-0.2654558872280859
1103	1	-0.006651844582634681
1103	2	-0.041748290245845254
1103	3	0.003728659008718438
1103	4	-0.11239235844619344
1103	5	0.082113396758416
1103	6	0.04215857291430325
1103	7	0.10877897966341266
1103	8	-6.915633554953615E-4
1103	9	0.08777112140065668
1103	10-11	0.04917291731084816
1103	12-13	0.14317618082359473
1103	14-15	0.13186318305918832
1103	16-17	0.03990308426142519
1103	18-19	0.11089417451613315
1103	20-21	0.06520535538754046
1103	22-23	-0.11791578134302227
1103	24-25	-0.019382102647966803
1103	26-27	-0.015061281239248103
1103	28-29	-0.23470391960968584
1103	30-31	-0.16629009907951087
1103	32-33	0.014086063893323342
1103	34-35	-0.09102717444137909
1103	36-37	-0.0603450942680297
1103	38-39	-0.09939997954383983
1103	40-41	-0.04446452822766389
1103	42-43	0.05719106057063783
1103	44-45	0.14653238335872487
1103	46-47	0.21250171913575855
1103	48-49	0.14307258552486246
1103	50-51	0.09353385668959646
1103	52-53	0.18821925772732584
1103	54-55	0.0902412624759279
1103	56-57	0.2514429973728056
1103	58-59	0.12064806520005078
1103	60-61	0.2394680671415017
1103	62-63	0.026025151004802183
1103	64-65	0.014690052674822596
1103	66-67	-0.051683867769298786
1103	68-69	0.003156482172371966
1103	70-71	0.09334817398370632
1103	72-73	0.18879540419493068
1103	74-75	0.1711194827405933
1103	76-77	0.15740073417291
1103	78-79	0.2722676339751793
1103	80-81	0.09538099440924697
1103	82-83	0.06728607833716893
1103	84-85	-0.10611846429575422
1103	86-87	-0.07841725745947059
1103	88-89	0.10143116329189539
1103	90-91	0.22575491914680867
1103	92-93	-0.017892732527482025
1103	94-95	-0.11791749582225108
1103	96-97	0.1468121043622368
1103	98-99	0.06749900040763279
1103	100-101	0.11921234071883191
1104	1	-0.06336801420334837
1104	2	-0.081489425947602
1104	3	0.0011594600146231926
1104	4	-0.16564015263374188
1104	5	-0.15935911808383452
1104	6	-0.14379228132492017
1104	7	0.002534650826682139
1104	8	-0.12704637725492063
1104	9	-0.2707042970771809
1104	10-11	-0.19163472115194935
1104	12-13	-0.06353998879712464
1104	14-15	0.0019451502723057956
1104	16-17	0.039406938492000165
1104	18-19	-0.15314773376008617
1104	20-21	-0.17657113380592193
1104	22-23	-0.11956733210912063
1104	24-25	0.00884808392790859
1104	26-27	0.1724676686989639
1104	28-29	0.059467096749195036
1104	30-31	-0.10759906837528632
1104	32-33	0.06372959077355489
1104	34-35	-0.05858541998870237
1104	36-37	0.01422994656434895
1104	38-39	-0.06782477883866989
1104	40-41	-0.009529838424093384
1104	42-43	-0.032169837136699186
1104	44-45	0.11885775473889737
1104	46-47	-0.03299829382689978
1104	48-49	0.12669124138355414
1104	50-51	0.15847720987020608
1104	52-53	0.1666114775394547
1104	54-55	0.012542436892822195
1104	56-57	0.022386441292027826
1104	58-59	0.06915379469528915
1104	60-61	0.10588743508225917
1104	62-63	0.08645587855563264
1104	64-65	-0.03486068317211277
1104	66-67	0.09638620836472
1104	68-69	0.00865092119170896
1104	70-71	0.25967529100194753
1104	72-73	0.3672700449132371
1104	74-75	0.08204819243752937
1104	76-77	0.15318603365353667
1104	78-79	0.17623474770954672
1104	80-81	0.18267283571173465
1104	82-83	0.29267009710709857
1104	84-85	0.21390938381644986
1104	86-87	0.09496608293130748
1104	88-89	0.16016680714716003
1104	90-91	0.10781092647502888
1104	92-93	-0.030391220217278914
1104	94-95	0.09399432353013282
1104	96-97	-5.373559590609034E-4
1104	98-99	-0.015497987934615765
1104	100-101	0.13950106234140947
1105	1	0.0032730524536255245
1105	2	0.0803545927388889
1105	3	0.06360106472623528
1105	4	0.09161313295330586
1105	5	0.012025372854672867
1105	6	0.024691077153988772
1105	7	-0.018815302820641477
1105	8	0.02007884769190582
1105	9	0.04774043416816198
1105	10-11	0.11921248768960169
1105	12-13	0.09866758032290335
1105	14-15	0.03950672662698196
1105	16-17	0.1174287242517309
1105	18-19	0.10621437156046909
1105	20-21	-0.06090301899669015
1105	22-23	-0.03615045791574545
1105	24-25	-0.02102144691025387
1105	26-27	0.07984706084263848
1105	28-29	0.16537683626530963
1105	30-31	0.09441409215040153
1105	32-33	0.08672597344674671
1105	34-35	0.1808859322371319
1105	36-37	0.027238689952277184
1105	38-39	0.19379633798826035
1105	40-41	0.1746423117949476
1105	42-43	0.4245535736934656
1105	44-45	0.4273407496673727
1105	46-47	0.2814914631396377
1105	48-49	0.35579163583577156
1105	50-51	0.3565476659442268
1105	52-53	0.4500015398883548
1105	54-55	0.36183942124197443
1105	56-57	0.32537096313231473
1105	58-59	0.21757530415668924
1105	60-61	0.3537134034897278
1105	62-63	0.2850496200349184
1105	64-65	0.2137007932061934
1105	66-67	0.19813443151197419
1105	68-69	0.2625645416087039
1105	70-71	0.32089400294275805
1105	72-73	0.25343240653684873
1105	74-75	0.10632904423619394
1105	76-77	0.09389027628709457
1105	78-79	0.11722846737580639
1105	80-81	0.3075751315327224
1105	82-83	0.1857751359161064
1105	84-85	0.23543043338655423
1105	86-87	0.33074853435188345
1105	88-89	0.3427943224617245
1105	90-91	0.47945353822134607
1105	92-93	0.3696749005178219
1105	94-95	0.32669204952217257
1105	96-97	0.46422710880381146
1105	98-99	0.5140870646893099
1105	100-101	0.5214735052185482
1106	1	-0.0031586477830352067
1106	2	0.07955804054425641
1106	3	0.06677127659123983
1106	4	0.012815159856351954
1106	5	0.05382201444382417
1106	6	0.10151719540881743
1106	7	9.940516098012608E-4
1106	8	-0.005909549840801276
1106	9	0.08225989159048908
1106	10-11	-0.21235483343902928
1106	12-13	-0.17286261855637264
1106	14-15	-0.09177500254526905
1106	16-17	0.11206599423729102
1106	18-19	0.004958849096567519
1106	20-21	0.035351350748435095
1106	22-23	-0.009955041501967798
1106	24-25	-0.18612582677108946
1106	26-27	-0.01645882837403434
1106	28-29	-0.01351311908447883
1106	30-31	-0.10140671418813696
1106	32-33	0.03278720398579793
1106	34-35	0.1022930960314099
1106	36-37	0.046593543839286156
1106	38-39	0.1395126938913549
1106	40-41	0.026101089160249558
1106	42-43	-0.011039794174386941
1106	44-45	-0.008326486301221792
1106	46-47	0.07990071123764864
1106	48-49	0.05562366824363352
1106	50-51	0.20549610412791708
1106	52-53	0.17318600782630256
1106	54-55	0.25337681873234175
1106	56-57	0.31391411480657894
1106	58-59	0.3255657624682584
1106	60-61	0.2835220051242402
1106	62-63	0.10026950702143012
1106	64-65	-0.01330529032592409
1106	66-67	0.10951764698569377
1106	68-69	0.04296879302372503
1106	70-71	0.1493887404287051
1106	72-73	0.21565781245853444
1106	74-75	0.24282317948539145
1106	76-77	-0.02135478231335952
1106	78-79	0.3447426881614426
1106	80-81	0.24123499010119076
1106	82-83	0.19396931962654662
1106	84-85	0.16604970213940362
1106	86-87	0.17715134604468474
1106	88-89	0.049921888437229
1106	90-91	-0.08096115823721917
1106	92-93	-0.04715432302022293
1106	94-95	0.09110079889288158
1106	96-97	-0.01558408056670757
1106	98-99	-0.13269873193345916
1106	100-101	-0.06834987003611204
1107	1	-0.24014197569892204
1107	2	-0.1441853162525497
1107	3	-0.1341213732512898
1107	4	-0.18421653358343804
1107	5	-0.1506629956125991
1107	6	-0.08357107258279939
1107	7	-0.10460613340289626
1107	8	-0.02237631720827693
1107	9	-0.09224932854274925
1107	10-11	-0.16163932050680785
1107	12-13	-0.07873149080747055
1107	14-15	0.024943243694607986
1107	16-17	-0.07837985722628815
1107	18-19	-0.093559046238326
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2309	4	0.014674029540337585
2309	5	0.03799337963469185
2309	6	-0.0274585776652998
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2309	8	0.022145517889832433
2309	9	0.09787285643628962
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2311	28-29	-0.18592982317149165
2311	30-31	-0.08043840031339045
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2312	60-61	0.18626659040219806
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2312	84-85	0.20737001984202408
2312	86-87	0.27663639308419263
2312	88-89	0.25635629797220005
2312	90-91	0.29171276003485147
2312	92-93	0.35514120770525537
2312	94-95	0.3894864269227867
2312	96-97	0.33016776604848985
2312	98-99	0.40603535130707513
2312	100-101	0.32579108026342496
2313	1	-0.1493157380661998
2313	2	-0.19241401796075408
2313	3	-0.13858640297847202
2313	4	-0.17471364371829878
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2313	24-25	-0.2922002680890827
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2313	28-29	-0.37384428743857256
2313	30-31	-0.39113656408991204
2313	32-33	-0.30199048251109417
2313	34-35	-0.2964488019941527
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2313	70-71	-0.16203169205189738
2313	72-73	-0.15980435669991166
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2313	78-79	-0.23335543148395388
2313	80-81	-0.2787006616396219
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2313	84-85	-0.1376189310421232
2313	86-87	-0.16617867636843187
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2313	100-101	-1.1916530234252214
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2314	2	-0.26647565115045424
2314	3	-0.2619140290308941
2314	4	-0.13725627819553665
2314	5	-0.23712161846088264
2314	6	-0.10561724085516744
2314	7	-0.14379617556060253
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2314	52-53	-0.13028127510742138
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2314	58-59	-0.03565820948742271
2314	60-61	0.23918473944495133
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2314	74-75	0.23476356189367564
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2314	78-79	0.06198424246607814
2314	80-81	-0.04657447719736041
2314	82-83	0.10789765776144833
2314	84-85	-2.496245992453794E-4
2314	86-87	-0.11706021989302684
2314	88-89	-0.26113448386302096
2314	90-91	0.016230896914517956
2314	92-93	0.10314626331832244
2314	94-95	-0.0028807078904797834
2314	96-97	0.03513823751349321
2314	98-99	0.1173997719012938
2314	100-101	-0.05413890266859411
2315	1	-0.8091005376494884
2315	2	-0.6944875112181279
2315	3	-0.5564613491909505
2315	4	-0.19806623571069082
2315	5	-0.2854675233744359
2315	6	-0.3135514064980356
2315	7	-0.1986455568192227
2315	8	-0.20947966380441585
2315	9	-0.5284306022999345
2315	10-11	-0.7814637664827018
2315	12-13	-0.6666402704296601
2315	14-15	-0.5629163106848694
2315	16-17	-0.44995690193481863
2315	18-19	-0.38713569234244005
2315	20-21	-0.38245567375962963
2315	22-23	-0.3407505373290789
2315	24-25	-0.36558783769831393
2315	26-27	-0.43480656052947353
2315	28-29	-0.40558270015935705
2315	30-31	-0.5212526696468203
2315	32-33	-0.32362785018120377
2315	34-35	-0.36042544248212494
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2315	38-39	-0.28550035767254656
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2315	56-57	-0.5343891217406878
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2315	60-61	-1.6478165440230228
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2315	64-65	-0.7289646408993491
2315	66-67	-0.6146697277613953
2315	68-69	-0.36341540652446724
2315	70-71	-0.38480545481306905
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2315	80-81	-0.7061903633736577
2315	82-83	-0.677103783566217
2315	84-85	-0.8199045295745719
2315	86-87	-0.7157371154104979
2315	88-89	-0.5325255023326072
2315	90-91	-0.5324255797943174
2315	92-93	-0.6853721891075715
2315	94-95	-0.8321259326346429
2315	96-97	-0.9689112068890076
2315	98-99	-1.631287418813809
2315	100-101	-1.4346540400408507
2316	1	-0.25989851466460223
2316	2	-0.23867634937933602
2316	3	-0.19921002414896805
2316	4	-0.2795267993127055
2316	5	-0.3274720101502524
2316	6	-0.3236335328624307
2316	7	-0.3068759042163691
2316	8	-0.3575727840814906
2316	9	-0.40420627662822994
2316	10-11	-0.4825110088210707
2316	12-13	-0.46775278092504635
2316	14-15	-0.7308022455610228
2316	16-17	-0.6504810150750373
2316	18-19	-0.37666162905529177
2316	20-21	-0.35135900473149917
2316	22-23	-0.4202801146275661
2316	24-25	-0.4620928754816873
2316	26-27	-0.7212263561319077
2316	28-29	-0.5057127445023397
2316	30-31	-0.5495770819054258
2316	32-33	-0.6305570981870758
2316	34-35	-0.731719260702107
2316	36-37	-0.5540129135986263
2316	38-39	-0.5461638298073055
2316	40-41	-0.7840736287838723
2316	42-43	-1.0537539103993154
2316	44-45	-1.1354148563465927
2316	46-47	-1.1697747006534698
2316	48-49	-1.3500946788194028
2316	50-51	-1.0703711430664455
2316	52-53	-1.0689707122668182
2316	54-55	-1.2005932296200896
2316	56-57	-1.1917353971919837
2316	58-59	-1.6091379296282042
2316	60-61	-1.2801009951145446
2316	62-63	-1.247703869291847
2316	64-65	-1.1296701273603063
2316	66-67	-0.9890564038739029
2316	68-69	-1.1160187532394161
2316	70-71	-1.287288596605677
2316	72-73	-1.1107603017964855
2316	74-75	-1.008482634745743
2316	76-77	-1.1373959639633142
2316	78-79	-0.9833869754608102
2316	80-81	-1.029332516763663
2316	82-83	-1.2612906866413631
2316	84-85	-1.1273938046060579
2316	86-87	-1.1817386372408905
2316	88-89	-1.029990211610528
2316	90-91	-1.3448960615323493
2316	92-93	-1.3888989654509771
2316	94-95	-1.3994628278382528
2316	96-97	-1.1834884725796009
2316	98-99	-1.3246935310619214
2316	100-101	-1.8168491163947529
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	23.0
5	72.0
6	149.0
7	362.0
8	861.0
9	1614.0
10	2762.0
11	4638.0
12	7504.0
13	9921.0
14	10409.0
15	12956.0
16	15656.0
17	19399.0
18	17314.0
19	12425.0
20	11216.0
21	11435.0
22	12563.0
23	13633.0
24	16143.0
25	19587.0
26	24097.0
27	30705.0
28	38168.0
29	47676.0
30	58549.0
31	71894.0
32	87820.0
33	109592.0
34	140762.0
35	184158.0
36	254816.0
37	349413.0
38	319804.0
39	64574.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5897149177694	20.058342550803737	13.93287932245537	21.419063208971494
2	15.514714263953913	22.227097890987444	39.27507548039628	22.983112364662365
3	20.247487285056913	27.16855372568015	30.029567288286106	22.55439170097683
4	12.648728091433576	17.23049393033541	39.01592199438249	31.104855983848523
5	12.044006013933322	38.89896198623211	34.78347267831358	14.273559321520995
6	28.18814287140907	38.38814226617562	18.411201660760597	15.01251320165471
7	26.616295015607278	33.01555583239061	23.012776834353055	17.35537231764906
8	24.696214157355847	34.13229947209124	20.542847761847433	20.628638608705476
9	24.643370144568493	18.636847817754123	23.00739183589479	33.7123902017826
10-11	24.326046635371316	26.481416335367154	28.518110829932077	20.674426199329456
12-13	26.40378488436897	24.241361275292792	28.46904443320292	20.885809407135316
14-15	22.391623032424132	26.28489951325848	28.22757845526333	23.095898999054054
16-17	20.47130495392966	29.2688511642287	28.89076116806684	21.369082713774805
18-19	20.183671091712483	29.347076680675976	30.58075511075896	19.88849711685258
20-21	21.42333453299736	27.567497494955234	29.898620403182953	21.110547568864458
22-23	20.71193289652797	27.491452437524238	30.084772049029397	21.711842616918396
24-25	21.605221893662723	27.62213091148614	30.202589461292995	20.57005773355814
26-27	21.447781693568604	26.69150310379805	31.517358016434216	20.343357186199132
28-29	20.558840805451865	28.358052362290415	30.940353777992545	20.142753054265178
30-31	21.033661788183192	27.787255077515177	30.24201582333551	20.93706731096612
32-33	21.081242211964693	27.77628563581766	30.44168831113152	20.700783841086125
34-35	21.95447717755676	26.854465374671005	30.669849074381382	20.52120837339085
36-37	21.999228681350306	26.902146702385764	31.003932514410515	20.094692101853408
38-39	21.427922899719498	27.896947756605396	30.88503003012849	19.790099313546623
40-41	20.91356357589654	28.11091149794534	30.405274697337205	20.570250228820917
42-43	20.604732164607466	28.496683106970245	29.44691382433	21.45167090409229
44-45	21.18506866041703	27.85282305347831	29.187473426776933	21.774634859327723
46-47	21.88839773070292	28.39148173842258	28.921130654045673	20.798989876828823
48-49	21.95426102104725	28.24972550459493	29.069681232281365	20.726332242076452
50-51	20.969784574694778	28.700316583859927	29.653472562311844	20.676426279133455
52-53	21.188019139277227	29.30405516840678	28.574858187881453	20.933067504434543
54-55	20.825387634921363	28.803255512982357	28.738218889217844	21.633137962878436
56-57	21.914651469923292	28.14482534092935	28.514143931108276	21.426379258039077
58-59	22.62343161125268	27.692181603372124	28.479758312728297	21.204628472646895
60-61	21.839684585723685	28.289833902091537	29.544489871681996	20.32599164050278
62-63	21.326724946977965	28.42957167981425	29.214214571456605	21.029488801751185
64-65	20.883300590184017	29.50899908897769	28.79448613270651	20.813214188131784
66-67	21.171649053137216	28.271366903716572	28.443466447525406	22.113517595620806
68-69	20.98758862923148	29.05583704628743	28.364254162762055	21.59232016171903
70-71	21.817215746708257	28.57132770017837	28.030884759388027	21.580571793725355
72-73	21.5553663662558	27.748623235954085	29.23798461615279	21.45802578163733
74-75	20.817348416588555	29.323505462296023	28.771765776302704	21.08738034481272
76-77	21.361013028003324	28.301901066661152	28.359145400102587	21.97794050523294
78-79	21.191221795817786	28.649225385819243	28.57347121063948	21.586081607723496
80-81	21.754037428725113	28.649610877132194	28.24564734909495	21.350704345047745
82-83	21.207512473968958	28.2579969325107	29.045169561750495	21.489321031769848
84-85	20.798944686310982	28.67390282479493	28.52393268264734	22.003219806246747
86-87	21.183215347029023	28.729681911075517	27.972362153732554	22.114740588162903
88-89	21.12499993695558	28.443624923022764	27.8722155170979	22.559159622923758
90-91	21.146258515409823	27.29687213935944	28.347469573501975	23.209399771728766
92-93	21.18880089008634	28.09277011633965	28.385447532164005	22.332981461410007
94-95	20.795192481064596	28.097154046635254	28.64420350249845	22.4634499698017
96-97	19.88434090784721	28.43495323478034	29.27314373385901	22.40756212351344
98-99	20.287287233519415	29.24034388121971	27.949692329146913	22.522676556113964
100-101	19.665981659908645	29.25088975644643	27.842158101644443	23.240970482000478
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	463.0
1	367.5
2	800.5
3	1129.0
4	764.0
5	607.0
6	740.0
7	1172.0
8	1666.5
9	1959.0
10	1991.5
11	1931.0
12	2108.0
13	2361.5
14	2566.5
15	2825.0
16	3071.0
17	3375.5
18	3696.5
19	3987.0
20	4315.0
21	4723.5
22	5412.5
23	6427.5
24	7753.5
25	9289.5
26	11306.5
27	14059.5
28	17627.0
29	21762.5
30	25873.0
31	30019.5
32	34350.0
33	38532.0
34	42464.5
35	46789.5
36	51389.0
37	56933.5
38	62566.0
39	66149.5
40	68936.0
41	71334.5
42	73830.0
43	77727.5
44	79495.5
45	80392.0
46	83413.5
47	96079.5
48	130138.0
49	128025.5
50	92528.0
51	74269.0
52	60014.0
53	50153.0
54	43546.5
55	37628.0
56	31401.5
57	25069.5
58	20362.5
59	16912.0
60	13673.5
61	10529.5
62	7735.5
63	5355.5
64	3485.0
65	2209.5
66	1338.0
67	814.5
68	479.0
69	260.0
70	156.0
71	104.5
72	65.5
73	45.0
74	27.0
75	17.5
76	21.0
77	21.5
78	15.5
79	9.5
80	7.0
81	3.5
82	1.0
83	0.5
84	0.5
85	1.0
86	1.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04967900303067136
2	0.00479137592681602
3	0.04080234868204379
4	0.0
5	0.0
6	0.0011600173296501942
7	0.0
8	0.0
9	5.0435536071747574E-5
10-11	3.278309844663592E-4
12-13	0.0
14-15	0.002748736715910243
16-17	0.0
18-19	0.0
20-21	0.01157495552846607
22-23	0.0
24-25	0.0
26-27	0.007968814699336116
28-29	0.010818422487389855
30-31	0.010793204719353982
32-33	0.006077482096645583
34-35	0.008220992379694855
36-37	0.02065335202138063
38-39	0.011095817935784466
40-41	0.014298474476340438
42-43	0.0
44-45	0.0
46-47	2.5217768035873787E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.001185235097686068
58-59	7.565330410762137E-5
60-61	0.009759276229883155
62-63	0.01505500751741665
64-65	0.04486240933581947
66-67	0.022494249087999418
68-69	0.0015635016182241749
70-71	1.765243762511165E-4
72-73	0.0
74-75	2.5217768035873787E-5
76-77	0.0
78-79	0.0
80-81	2.5217768035873787E-5
82-83	0.0
84-85	5.0435536071747574E-5
86-87	6.556619689327184E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	7.565330410762137E-5
96-97	2.7739544839461164E-4
98-99	4.7913759268160196E-4
100-101	0.001185235097686068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1982729.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.04060121136878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37056130936753	43.13562811740025
2	13.35078846944836	14.696708480084956
3	3.5586087324371407	5.8760389232810155
4	1.4380676940489536	3.1660844185240453
5	0.7853233953378492	2.1612335912374334
6	0.4894439384928983	1.6163573180345594
7	0.34224666264433856	1.3186223452170232
8	0.2462270429161933	1.0841987581283825
9	0.18205723658691755	0.9018485780961911
>10	1.1084019461287036	11.416571743313577
>50	0.08426282767321508	3.202785209803849
>100	0.03785620835919549	3.6585730130199856
>500	0.002663903883657733	1.0100799046025057
>1k	0.0027557626382666194	3.3302098846958277
>5k	5.511525276533238E-4	1.8245952346228465
>10k+	1.837175092177746E-4	1.6004644799375474
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	21323	1.0754369356578735	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	10332	0.521099958693296	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	7411	0.37377775782772127	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7122	0.35920188790298624	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5738	0.2893991059796876	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	5472	0.2759832533846028	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	5304	0.2675100833245491	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	5041	0.2542455373376796	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	4684	0.23624005096006565	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	4376	0.22070590584996738	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3898	0.19659771960767206	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	3898	0.19659771960767206	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	3771	0.1901924065265601	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	3550	0.1790461530547039	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	3277	0.1652772517071168	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	2933	0.14792742729843567	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	2923	0.14742307193771817	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2822	0.14232908279447168	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	2663	0.1343098325590638	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	2138	0.10783117612139631	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2006	0.10117368535992564	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.0435536071747574E-5	0.0
10-11	0.0	0.0	0.0	5.0435536071747574E-5	0.0
12-13	0.0	0.0	0.0	5.0435536071747574E-5	0.0
14-15	0.0	0.0	0.0	1.5130660821524274E-4	0.0
16-17	0.0	0.0	0.0	1.5130660821524274E-4	0.0
18-19	0.0	0.0	0.0	2.521776803587379E-4	0.0
20-21	0.0	0.0	0.0	3.782665205381068E-4	0.0
22-23	0.0	0.0	0.0	8.574041132197088E-4	0.0
24-25	0.0	0.0	0.0	0.0021939458191210194	0.0
26-27	0.0	0.0	0.0	0.004513980478421407	0.0
28-29	0.0	0.0	0.0	0.011171471239892088	0.0
30-31	0.0	0.0	0.0	0.02637778536552398	0.0
32-33	0.0	0.0	0.0	0.046249386577792526	0.0
34-35	0.0	2.5217768035873787E-5	0.0	0.06208614490432127	0.0
36-37	0.0	5.0435536071747574E-5	0.0	0.08392473202338796	0.0
38-39	0.0	1.0087107214349516E-4	0.0	0.1190026473612884	0.0
40-41	0.0	1.5130660821524274E-4	0.0	0.1618224174862021	0.0
42-43	0.0	1.5130660821524274E-4	0.0	0.20073343356555534	0.0
44-45	0.0	1.5130660821524274E-4	0.0	0.21631801421172536	0.0
46-47	0.0	1.5130660821524274E-4	0.0	0.23762702820203868	0.0
48-49	0.0	1.5130660821524274E-4	0.0	0.2673587767163339	0.0
50-51	0.0	1.5130660821524274E-4	0.0	0.3008227548999384	0.0
52-53	0.0	1.5130660821524274E-4	0.0	0.37226469174556887	0.0
54-55	0.0	1.5130660821524274E-4	0.0	0.4075191314597204	0.0
56-57	0.0	1.5130660821524274E-4	0.0	0.5130554906898522	0.0
58-59	0.0	1.5130660821524274E-4	0.0	0.7425119620482679	0.0
60-61	0.0	1.5130660821524274E-4	0.0	0.9072344228585953	0.0
62-63	0.0	1.5130660821524274E-4	0.0	0.9787772307763694	0.0
64-65	0.0	1.5130660821524274E-4	0.0	1.1511658930696025	0.0
66-67	0.0	1.5130660821524274E-4	0.0	1.5391412543015208	0.0
68-69	0.0	1.5130660821524274E-4	0.0	1.9598744962120391	0.0
70-71	0.0	1.5130660821524274E-4	0.0	2.2479118427177895	0.0
72-73	0.0	1.5130660821524274E-4	0.0	2.5565268879408127	0.0
74-75	0.0	1.5130660821524274E-4	0.0	2.915350509323261	0.0
76-77	0.0	1.5130660821524274E-4	0.0	3.060075280081141	0.0
78-79	0.0	1.5130660821524274E-4	0.0	3.268651439505853	0.0
80-81	0.0	1.5130660821524274E-4	0.0	3.566321973401307	0.0
82-83	0.0	1.5130660821524274E-4	0.0	3.8195840177855875	0.0
84-85	0.0	1.5130660821524274E-4	0.0	4.311910503149951	0.0
86-87	0.0	1.5130660821524274E-4	0.0	5.2483975369301605	0.0
88-89	0.0	1.5130660821524274E-4	0.0	6.347715698918007	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	355	0.0	52.20461	2
TACCTGG	2180	0.0	51.443214	2
ACCTGGG	2565	0.0	42.607998	3
TAACGCA	465	0.0	40.854378	4
ATAACGC	540	0.0	37.837757	3
GTACCTG	3050	0.0	37.575756	1
GTATAAC	655	0.0	34.12299	1
GTACACG	330	0.0	25.938753	1
GCGTTAG	110	9.406567E-5	25.938753	1
CCTGGGG	2085	0.0	24.600803	4
TACACGG	300	0.0	23.759789	2
TACGCAG	400	0.0	21.371946	4
CTGGGGG	1410	0.0	20.546711	5
AGTACTT	8340	0.0	20.216957	12-13
AACCGTA	120	0.0047700247	19.787844	7
GAGTACT	8130	0.0	19.687603	12-13
CCGGTCG	315	3.2196112E-10	19.601364	9
TCACGCA	340	4.1836756E-11	19.55603	4
CATGGGG	2860	0.0	18.764801	4
CCCGGTC	380	1.0913936E-11	18.746376	8
>>END_MODULE