##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003815_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1382417 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.762502197238604 31.0 31.0 34.0 27.0 34.0 2 30.69935771912527 31.0 31.0 34.0 27.0 34.0 3 30.78245203871191 31.0 31.0 34.0 27.0 34.0 4 34.316829147789704 37.0 35.0 37.0 30.0 37.0 5 34.30675331683566 37.0 35.0 37.0 30.0 37.0 6 34.39673846603449 37.0 35.0 37.0 30.0 37.0 7 34.38149849141033 37.0 35.0 37.0 30.0 37.0 8 34.364473237814636 37.0 35.0 37.0 30.0 37.0 9 35.80473113394873 39.0 35.0 39.0 30.0 39.0 10-11 35.66965792521359 39.0 35.0 39.0 30.0 39.0 12-13 35.55237710473757 38.0 35.0 39.0 30.0 39.0 14-15 36.50976007962865 39.0 36.0 41.0 30.0 41.0 16-17 36.54577851690192 39.0 36.0 41.0 30.0 41.0 18-19 36.478023273729995 39.0 36.0 41.0 29.5 41.0 20-21 36.4019420334096 39.0 36.0 41.0 29.0 41.0 22-23 36.29953986387609 39.0 36.0 41.0 29.0 41.0 24-25 36.08387121975497 39.0 35.0 41.0 27.0 41.0 26-27 35.92446960649356 39.0 35.0 40.5 27.0 41.0 28-29 35.762723186997846 39.0 35.0 40.0 27.0 41.0 30-31 35.484947740081324 38.0 34.5 40.0 26.5 41.0 32-33 35.203373873440505 38.0 34.0 40.0 26.0 41.0 34-35 35.17686197435361 38.0 34.0 40.0 25.5 41.0 36-37 35.01900909783372 38.0 34.0 40.0 25.0 41.0 38-39 35.00158779876115 38.0 34.0 40.0 25.0 41.0 40-41 35.12751506962081 38.0 35.0 40.0 25.0 41.0 42-43 34.9889787234966 38.0 34.0 40.0 24.5 41.0 44-45 34.75505003193682 38.0 34.0 40.0 24.0 41.0 46-47 34.481294356189196 38.0 34.0 40.0 23.0 41.0 48-49 34.194624342727266 38.0 33.0 40.0 22.0 41.0 50-51 33.34307629318795 37.5 32.5 39.5 19.0 40.5 52-53 33.49533534382172 38.0 33.0 39.5 20.0 40.5 54-55 33.827897805076184 38.0 33.0 40.0 19.5 41.0 56-57 33.607825641611754 38.0 33.0 40.0 18.0 41.0 58-59 33.33234074812448 38.0 32.5 40.0 15.5 41.0 60-61 32.996093436350975 37.5 32.0 40.0 13.0 41.0 62-63 32.76749779552769 37.0 32.0 40.0 13.0 41.0 64-65 32.47847574212412 36.5 31.0 39.5 10.5 41.0 66-67 32.07075867845954 36.0 31.0 39.0 8.0 41.0 68-69 31.664550566146104 35.5 31.0 39.0 7.5 40.5 70-71 31.182951309192525 35.0 30.0 38.5 6.5 40.0 72-73 30.78764981912115 35.0 30.0 37.5 2.0 40.0 74-75 30.434101287816915 35.0 29.5 37.0 2.0 39.5 76-77 30.020349503803843 34.5 29.0 36.5 2.0 39.0 78-79 29.47470191700478 34.0 29.0 36.0 2.0 39.0 80-81 29.020845012756645 34.0 28.5 36.0 2.0 37.5 82-83 28.632416991399843 34.0 27.5 35.0 2.0 37.0 84-85 28.367071223805844 34.0 27.0 35.0 2.0 37.0 86-87 28.19072718289778 34.0 27.0 35.0 2.0 36.0 88-89 27.851722020200853 34.0 26.5 35.0 2.0 36.0 90-91 27.499473747791008 34.0 26.0 35.0 2.0 36.0 92-93 27.24518072332733 33.5 25.0 35.0 2.0 35.0 94-95 26.99290698826765 33.0 25.0 35.0 2.0 35.0 96-97 26.6626980136963 33.0 25.0 35.0 2.0 35.0 98-99 26.427914297928915 33.0 25.0 35.0 2.0 35.0 100-101 26.11371424107198 33.0 24.0 35.0 2.0 35.0 102-103 25.73343462934845 32.5 21.5 35.0 2.0 35.0 104-105 25.360774643251638 32.0 19.5 35.0 2.0 35.0 106-107 24.939448082597366 32.0 18.5 34.5 2.0 35.0 108-109 24.617367624964103 32.0 17.0 34.0 2.0 35.0 110-111 24.250501115075984 31.5 9.0 34.0 2.0 35.0 112-113 23.713581719553506 31.0 4.5 34.0 2.0 35.0 114-115 23.201527107956572 31.0 2.0 34.0 2.0 35.0 116-117 22.52577225251136 31.0 2.0 34.0 2.0 35.0 118-119 21.731681178689207 30.5 2.0 34.0 2.0 35.0 120-121 20.11781502976309 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5526899712386744 1101 2 0.490816610433761 1101 3 0.5175151119574508 1101 4 0.7389974432398461 1101 5 0.7559884942849919 1101 6 0.8458494816665905 1101 7 0.5234841888208877 1101 8 0.574348089467513 1101 9 0.7995511201751739 1101 10-11 0.7828601375923014 1101 12-13 0.8287445678926844 1101 14-15 1.0333051363545067 1101 16-17 0.834136893068397 1101 18-19 0.7540513623707952 1101 20-21 0.8811335856685716 1101 22-23 0.764168187655109 1101 24-25 0.8239764215146366 1101 26-27 0.8018682241925816 1101 28-29 0.8266738773267122 1101 30-31 0.7730971885483839 1101 32-33 0.8576859744563308 1101 34-35 0.8892446402633141 1101 36-37 1.0171510194219593 1101 38-39 0.8544635459195078 1101 40-41 0.7199154830721639 1101 42-43 0.6596076928585077 1101 44-45 0.7973023514088169 1101 46-47 0.8041205755606811 1101 48-49 0.9275548917539638 1101 50-51 0.5763886567986489 1101 52-53 0.6442516776010123 1101 54-55 0.7993555280973794 1101 56-57 0.9265931189642416 1101 58-59 0.9261056931304381 1101 60-61 0.7057589765306744 1101 62-63 0.7981773635361833 1101 64-65 0.8883762262356072 1101 66-67 0.7506940924185947 1101 68-69 0.820701824931092 1101 70-71 0.5903017317329784 1101 72-73 0.6196149830176267 1101 74-75 0.5353152073050964 1101 76-77 0.5305605859493809 1101 78-79 0.7682372924311451 1101 80-81 0.8217858348801776 1101 82-83 0.7103462572619748 1101 84-85 0.6703062267260549 1101 86-87 0.7902449189660885 1101 88-89 0.824218095228396 1101 90-91 0.8790405885731083 1101 92-93 0.8552730651877383 1101 94-95 0.8148350259536095 1101 96-97 0.8780582097840082 1101 98-99 0.710907526292818 1101 100-101 0.6598915764872508 1101 102-103 0.7580595385340168 1101 104-105 0.7713611022000713 1101 106-107 0.6595884560261069 1101 108-109 0.9100181088155566 1101 110-111 0.7125512045588529 1101 112-113 0.6858473017992068 1101 114-115 0.5482913852243954 1101 116-117 0.6894105565605635 1101 118-119 0.8576600610227771 1101 120-121 0.8651112638310892 1102 1 0.5516100240802757 1102 2 0.6451999655773015 1102 3 0.6386195660870371 1102 4 0.8667089618891239 1102 5 0.8824814994834043 1102 6 0.8997238076316805 1102 7 0.8260067167174512 1102 8 0.8958206452637469 1102 9 0.9473423237632375 1102 10-11 0.9088554226101238 1102 12-13 0.9043029651964147 1102 14-15 0.9319749620840909 1102 16-17 1.0187522442252046 1102 18-19 0.9376302625148298 1102 20-21 1.1074213511970044 1102 22-23 1.0374237125976578 1102 24-25 1.21764232637738 1102 26-27 1.055179523621291 1102 28-29 1.0181215409001823 1102 30-31 0.9700896786281632 1102 32-33 0.9190963183368552 1102 34-35 0.8651772908314399 1102 36-37 1.0354197112691637 1102 38-39 0.9640104527843221 1102 40-41 1.0661398312202834 1102 42-43 1.1142666892905808 1102 44-45 1.159503455216715 1102 46-47 1.109365030112805 1102 48-49 1.1501675436642458 1102 50-51 0.882982113238505 1102 52-53 0.7864710249351319 1102 54-55 0.9055793294368115 1102 56-57 0.9553064781218552 1102 58-59 1.0459127193735256 1102 60-61 1.331097469749622 1102 62-63 1.117902617025507 1102 64-65 1.0314157866014213 1102 66-67 1.1470791277561112 1102 68-69 1.0381962232489101 1102 70-71 0.9737451103147308 1102 72-73 0.9238876094018877 1102 74-75 0.9628326088702153 1102 76-77 0.7814252271869577 1102 78-79 1.199858866022275 1102 80-81 1.214485503936647 1102 82-83 1.2695771335601798 1102 84-85 1.1670020730927 1102 86-87 1.1151086206869216 1102 88-89 1.0175270831476553 1102 90-91 1.2354697538572132 1102 92-93 1.3019393341753585 1102 94-95 1.1574687672807933 1102 96-97 1.2067262708128297 1102 98-99 1.1129399147655938 1102 100-101 1.2694561924970635 1102 102-103 1.406277936409417 1102 104-105 1.3364412780239192 1102 106-107 1.089532631830071 1102 108-109 1.1781901770821328 1102 110-111 1.2545482865888538 1102 112-113 1.1897218763136976 1102 114-115 1.1984810905566476 1102 116-117 1.4093972840224147 1102 118-119 1.1977797251331097 1102 120-121 1.1232257804832777 1103 1 0.4840350897809671 1103 2 0.570233259845363 1103 3 0.6813698501981236 1103 4 0.7889428740988009 1103 5 0.9901276391240046 1103 6 0.8970176495238604 1103 7 0.8022563615785927 1103 8 0.7446939672326423 1103 9 0.8856564795981683 1103 10-11 0.9615724568455093 1103 12-13 1.030883333120272 1103 14-15 1.0609440827602796 1103 16-17 1.014079282722257 1103 18-19 0.8742066795188848 1103 20-21 1.037965512714159 1103 22-23 1.0551697794347916 1103 24-25 1.1454803297867144 1103 26-27 1.1082301664226293 1103 28-29 1.050122215664011 1103 30-31 0.9489265100792608 1103 32-33 0.9750295788667174 1103 34-35 0.9637001351385663 1103 36-37 0.9245031866580433 1103 38-39 1.029505605138887 1103 40-41 1.0866841703068673 1103 42-43 0.9311806213880303 1103 44-45 1.0115774661549892 1103 46-47 1.029416392070324 1103 48-49 1.0711344979933912 1103 50-51 0.8448954682353005 1103 52-53 0.8855445564359528 1103 54-55 0.7127142910818094 1103 56-57 0.8161022555208177 1103 58-59 0.9038895820768502 1103 60-61 0.8821859703533619 1103 62-63 1.091299387037587 1103 64-65 0.9888663779076268 1103 66-67 1.0836071570905794 1103 68-69 1.0788889215939577 1103 70-71 0.905936006330073 1103 72-73 0.734203061493659 1103 74-75 0.6564682985498784 1103 76-77 0.6602236592488957 1103 78-79 0.7987162067424975 1103 80-81 0.7231635439252848 1103 82-83 0.6997870206260224 1103 84-85 0.9778622194099071 1103 86-87 1.0152415757973685 1103 88-89 0.8403458646662259 1103 90-91 0.9950991220651879 1103 92-93 0.8993694606047917 1103 94-95 0.9086045457451704 1103 96-97 0.7872222666221944 1103 98-99 0.9916921787758213 1103 100-101 0.9978435513293675 1103 102-103 1.176497545401535 1103 104-105 1.2746164857801503 1103 106-107 1.2185101870541644 1103 108-109 1.3565515663853525 1103 110-111 1.459856502726364 1103 112-113 1.4136819853413272 1103 114-115 1.302971821432422 1103 116-117 1.0068609266816928 1103 118-119 1.0069631721108792 1103 120-121 1.0921131748294677 1104 1 0.5702131567822519 1104 2 0.6752260130456271 1104 3 0.6653395887297009 1104 4 0.9385384637645728 1104 5 1.0184747851494507 1104 6 0.8533189607568872 1104 7 0.6449081552831188 1104 8 0.841539907079337 1104 9 0.8726229400471723 1104 10-11 0.845896631974874 1104 12-13 0.9393353388788057 1104 14-15 1.078613871873543 1104 16-17 0.926705128992829 1104 18-19 0.8577577816160726 1104 20-21 0.9269389475999574 1104 22-23 0.9298160723805964 1104 24-25 1.064351434023905 1104 26-27 1.0290756588159127 1104 28-29 0.9069483119248574 1104 30-31 1.0402913888777547 1104 32-33 1.1096127419732156 1104 34-35 0.9319762965719747 1104 36-37 0.9462253472743569 1104 38-39 0.7015118013958457 1104 40-41 0.7771677604395464 1104 42-43 0.9593879382239479 1104 44-45 0.9933622216119744 1104 46-47 1.0325666829150393 1104 48-49 1.0483014856592447 1104 50-51 0.8233920491076461 1104 52-53 0.9488487566616186 1104 54-55 0.8157050550598655 1104 56-57 0.9502514617773841 1104 58-59 1.077096120732925 1104 60-61 0.8273739007840817 1104 62-63 0.9681822174721972 1104 64-65 0.9009178301857474 1104 66-67 1.0920091891126766 1104 68-69 1.1238740389045745 1104 70-71 0.9790996235044993 1104 72-73 0.8235694771973279 1104 74-75 0.7998628892272848 1104 76-77 0.7402172635833573 1104 78-79 0.8385809007232758 1104 80-81 0.9251494831883456 1104 82-83 0.9868643717018593 1104 84-85 0.8733273764076905 1104 86-87 0.875029497473566 1104 88-89 0.9993971904901251 1104 90-91 1.140346753480305 1104 92-93 1.222959739193172 1104 94-95 1.1110306429787435 1104 96-97 1.0872540017686845 1104 98-99 1.2011496172023435 1104 100-101 1.0894655942459828 1104 102-103 1.1319412781356668 1104 104-105 1.2151419030790898 1104 106-107 1.2468602512540023 1104 108-109 1.382675063525543 1104 110-111 1.279249595407581 1104 112-113 1.3280169090797038 1104 114-115 1.314474292153406 1104 116-117 1.2189831446887744 1104 118-119 1.123111133279707 1104 120-121 1.1798832275511515 1105 1 0.7198104927920532 1105 2 0.7689428377124301 1105 3 0.8207889938298223 1105 4 0.9949617175901082 1105 5 1.2387285976368574 1105 6 1.2205060553911196 1105 7 1.0119198533731364 1105 8 1.0707281203042385 1105 9 1.2576817010727268 1105 10-11 1.3293873924605535 1105 12-13 1.2856214364266592 1105 14-15 1.5116439060647267 1105 16-17 1.4138134829933477 1105 18-19 1.1282251417834885 1105 20-21 1.248232310893954 1105 22-23 1.3062055965912052 1105 24-25 1.3444337717192312 1105 26-27 1.3695483636439079 1105 28-29 1.3163340324815564 1105 30-31 1.144913040069092 1105 32-33 1.1899801706757955 1105 34-35 1.2522949516656467 1105 36-37 1.1003349265040967 1105 38-39 0.9507335787071725 1105 40-41 1.0378457015069458 1105 42-43 0.9802744515666646 1105 44-45 1.0891516540816042 1105 46-47 1.2920442240401186 1105 48-49 1.2137492895894653 1105 50-51 0.929127356941315 1105 52-53 1.1609502905696516 1105 54-55 1.4036957077928562 1105 56-57 1.2843064357018719 1105 58-59 1.3304160016924769 1105 60-61 1.2420724495129676 1105 62-63 1.412774098999634 1105 64-65 1.4715887904070186 1105 66-67 1.2557366107866592 1105 68-69 1.1473166942618178 1105 70-71 0.8675405774197777 1105 72-73 0.9966604004102493 1105 74-75 1.0741159180009134 1105 76-77 1.0448512975797257 1105 78-79 1.0278304295241227 1105 80-81 1.3830501743653265 1105 82-83 1.4057692542514282 1105 84-85 1.4742774781599657 1105 86-87 1.3799495137844922 1105 88-89 1.3482688750259122 1105 90-91 1.3889836103742326 1105 92-93 1.4081180083125915 1105 94-95 1.4091051327132682 1105 96-97 1.3031564838366947 1105 98-99 1.4566533602155296 1105 100-101 1.5344493326625717 1105 102-103 1.635563328187029 1105 104-105 1.5891185310916676 1105 106-107 1.5947809427302069 1105 108-109 1.87488917591239 1105 110-111 1.5228295566548162 1105 112-113 1.9048488718688965 1105 114-115 1.7526075381386192 1105 116-117 1.758215945561492 1105 118-119 1.6853842279273081 1105 120-121 1.864813258336266 1106 1 0.45591245112296264 1106 2 0.5468329903375277 1106 3 0.5236293711290614 1106 4 0.7179108731920465 1106 5 0.8571897518639133 1106 6 0.9003877568232355 1106 7 0.8607071323206199 1106 8 0.8517496571223191 1106 9 0.798244006674885 1106 10-11 0.8388196673134942 1106 12-13 0.782149012431141 1106 14-15 0.8739796351253943 1106 16-17 0.7655050610205762 1106 18-19 0.7150662394906746 1106 20-21 0.9749557776882156 1106 22-23 0.7505536706745346 1106 24-25 0.9902283903378262 1106 26-27 0.8553285493756277 1106 28-29 0.9794664552489181 1106 30-31 0.8977568586763098 1106 32-33 0.9317485413874991 1106 34-35 0.7748625136081628 1106 36-37 0.9387956026572368 1106 38-39 1.0371963386032022 1106 40-41 1.0080070885477639 1106 42-43 1.1647277348835772 1106 44-45 1.000235468270759 1106 46-47 1.212665819051331 1106 48-49 1.2939560812889468 1106 50-51 0.9662147657518076 1106 52-53 1.084674008352728 1106 54-55 1.2180381727502407 1106 56-57 1.0494825008485122 1106 58-59 1.1728694193646376 1106 60-61 1.4258626113286255 1106 62-63 1.3558948419265064 1106 64-65 1.2182712252704775 1106 66-67 1.4467329127200195 1106 68-69 1.1895149605844466 1106 70-71 1.07743728460321 1106 72-73 1.0880640130829988 1106 74-75 1.2316113958749497 1106 76-77 1.3482310329328406 1106 78-79 1.3155384071354241 1106 80-81 1.1653936267926461 1106 82-83 1.282096476069075 1106 84-85 1.17776902511239 1106 86-87 1.1679764820993164 1106 88-89 1.4115197035156584 1106 90-91 1.2785629588996024 1106 92-93 1.4888564511846099 1106 94-95 1.077608468721344 1106 96-97 1.09807987455126 1106 98-99 1.2513760423091433 1106 100-101 1.1067736744969672 1106 102-103 1.222106866090666 1106 104-105 1.2690377365987509 1106 106-107 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-0.45151265713566247 2316 88-89 -0.4329396622189847 2316 90-91 -0.698821615249539 2316 92-93 -0.7490773369717516 2316 94-95 -0.8186650772127422 2316 96-97 -0.7662469127567988 2316 98-99 -0.6488212503056481 2316 100-101 -0.2948079822636238 2316 102-103 -0.6839221641083668 2316 104-105 -1.0792776773703423 2316 106-107 -1.2109093736945091 2316 108-109 -1.1581947281077767 2316 110-111 -1.117826270723814 2316 112-113 -0.9859417163788926 2316 114-115 -0.5023538304122184 2316 116-117 -0.8995604868204161 2316 118-119 -0.9932011414236861 2316 120-121 -1.2828709347669864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18601.0 3 2773.0 4 2467.0 5 2757.0 6 3355.0 7 3751.0 8 4835.0 9 5868.0 10 7968.0 11 10021.0 12 10459.0 13 11741.0 14 14059.0 15 14186.0 16 11054.0 17 10878.0 18 11302.0 19 12289.0 20 13026.0 21 14716.0 22 16044.0 23 17913.0 24 20271.0 25 23212.0 26 26600.0 27 30526.0 28 36023.0 29 42084.0 30 49049.0 31 58741.0 32 70115.0 33 86693.0 34 109551.0 35 141391.0 36 186452.0 37 205737.0 38 75451.0 39 458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.033871333732854 18.804292172617835 13.29754583379395 21.864290659855357 2 14.463774675855626 21.747021376007474 39.049687536502745 24.739516411634156 3 21.62557052311234 25.451111722230284 30.04359938171598 22.879718372941394 4 11.424405713552181 16.191169434363 41.62375570363282 30.760669148451996 5 11.080337057342348 38.52905047982237 35.55171548532231 14.838896977512977 6 27.147781864229 37.62967512469674 18.84835505344563 16.37418795762864 7 25.48740453756102 32.610939187755136 23.98429277749293 17.91736349719092 8 23.351785401715386 31.550557208705293 21.487615963591807 23.610041425987514 9 22.843624778823024 19.29523862315236 25.113670013434724 32.74746658458989 10-11 23.879683158698167 25.061255717595373 28.992999398656476 22.066061725049988 12-13 26.935622336379 23.46324157433267 28.14968282715265 21.45145326213568 14-15 22.336176476165885 25.621180757655992 29.386083917206047 22.65655884897208 16-17 19.81248137561317 28.987989572896534 29.49942389779192 21.70010515369838 18-19 19.062365368599572 30.392798111539825 30.07903968983844 20.465796830022168 20-21 21.113968231849206 27.440859242278936 28.503421188276068 22.94175133759579 22-23 19.81212660573213 27.556806991180782 29.522675381327186 23.108391021759907 24-25 22.04452812241039 27.18967745888724 29.129658376031465 21.636136042670902 26-27 21.22871243684992 25.58136735720359 32.18256815248152 21.00735205346497 28-29 19.53968019464827 29.372335943356482 30.263938818576946 20.82404504341831 30-31 20.4799656198048 27.44669774649932 29.44103362393305 22.632303009762822 32-33 20.726322202643495 27.3516911279172 30.44088622326318 21.48110044617613 34-35 22.275501753080203 26.138845411526912 30.186253370255645 21.399399465137236 36-37 21.74561221719853 26.17757932743528 31.487942171459927 20.588866283906256 38-39 20.979498827806058 28.103796624169014 30.81396154853642 20.10274299948851 40-41 20.267783391562975 28.724362173789945 30.123843798615912 20.88401063603116 42-43 19.601083099661366 28.807977462420954 28.333811257260976 23.257128180656704 44-45 20.753285953967275 27.217428388292888 28.43196089797774 23.5973247597621 46-47 22.13529895681137 28.508362472935282 28.802002104982826 20.55433646527052 48-49 21.584744009710107 27.69434901302817 29.415835692821133 21.305071284440587 50-51 19.183847978569318 28.318723330766588 31.274058674412906 21.223370016251188 52-53 20.341142470818347 29.459120148537917 28.36767001757485 21.832067363068887 54-55 19.639286015300296 28.071744363091454 29.135000844665164 23.153968776943085 56-57 21.524427361681443 26.78955404937879 28.89460476971115 22.791413819228616 58-59 23.18760642791793 26.656737042241396 28.394384837514576 21.7612716923261 60-61 21.538595701935577 27.46314805834719 30.666646233401163 20.331610006316065 62-63 20.223863822135034 28.122701078960276 30.157574616408606 21.495860482496088 64-65 19.515407025113042 30.26642652180132 28.93217756319128 21.28598888989436 66-67 20.469284123318904 27.810318806395394 27.73529523381374 23.985101836471966 68-69 19.96992059723782 28.43018144354127 28.613606678050758 22.986291281170153 70-71 22.11726184520075 27.6009191164387 28.06862112813801 22.21319791022254 72-73 21.1675028093591 26.25827697267773 30.87216311337978 21.702057104583393 74-75 19.72395995589612 29.840278647709837 29.080257304294506 21.35550409209954 76-77 20.460043827611397 27.779181884587288 28.502373995617237 23.258400292184074 78-79 21.03505989298021 27.94744191172875 29.024354994463614 21.99314320082743 80-81 22.28282887269197 27.272627733230166 28.328363547441686 22.11617984663618 82-83 21.215186158670413 26.485475123180493 30.599497240370134 21.699841477778968 84-85 20.633059061745225 27.678052422375572 29.66898755724837 22.019900958630835 86-87 21.250745286178947 28.369842929797507 28.60807908462437 21.771332699399174 88-89 20.153906165332543 27.640639430527486 27.839216260092588 24.366238144047383 90-91 20.350036821780392 26.0442021935417 28.774779890417797 24.830981094260107 92-93 21.828016512972052 27.543113373237617 29.133433878596154 21.495436235194173 94-95 20.853574299504146 27.027322886908813 29.517030143516404 22.60207267007064 96-97 18.40209750018702 27.974040333454365 31.048695357073136 22.575166809285488 98-99 20.19835572201585 28.756689049266292 28.34821086408903 22.696744364628827 100-101 19.3001420751586 27.37674806640198 29.189310313240508 24.133799545198915 102-103 20.94848391303618 26.035762268923445 29.26351794645001 23.75223587159036 104-105 22.62536045452207 26.151110125127847 28.95359728413504 22.269932136215047 106-107 21.452637985502516 26.711860918757623 30.146930578330256 21.6885705174096 108-109 20.531213500143945 26.692179393291887 29.708862279076097 23.067744827488067 110-111 20.260442246098183 28.271838099484576 28.067470224173423 23.400249430243818 112-113 21.2292739104241 26.539976872386145 27.251831544269965 24.97891767291978 114-115 20.228145512187076 26.754825130526292 28.17397472818273 24.843054629103897 116-117 22.51334714548635 26.795491695738 27.042307595455494 23.648853563320156 118-119 22.276892757431433 25.788106056458826 28.851334829656395 23.08366635645335 120-121 21.48356149637001 28.23324630113142 27.39368660744098 22.889505595057592 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3312.0 1 3393.0 2 2289.0 3 1066.0 4 944.0 5 855.5 6 881.5 7 942.5 8 1058.5 9 1170.0 10 1125.5 11 1094.0 12 1149.0 13 1227.0 14 1321.0 15 1458.0 16 1636.5 17 1818.0 18 1990.0 19 2169.5 20 2452.5 21 2902.5 22 3443.0 23 4200.0 24 5260.5 25 6550.0 26 8106.5 27 10077.0 28 12688.0 29 15807.0 30 19064.5 31 22322.0 32 25676.5 33 28749.5 34 31383.5 35 33924.0 36 36449.5 37 39510.5 38 42328.0 39 43557.0 40 44301.5 41 44847.5 42 45184.5 43 46751.5 44 47744.0 45 48795.5 46 53221.5 47 71240.5 48 109981.5 49 108588.0 50 70112.5 51 49850.0 52 38613.5 53 31791.5 54 27245.0 55 23910.5 56 20691.5 57 17161.5 58 14243.5 59 11733.0 60 9325.0 61 7142.5 62 5316.5 63 3816.5 64 2548.5 65 1665.5 66 1057.5 67 640.0 68 373.5 69 231.5 70 150.5 71 97.5 72 71.0 73 48.5 74 36.5 75 30.5 76 27.5 77 30.0 78 25.0 79 18.0 80 14.0 81 7.0 82 4.5 83 4.5 84 4.0 85 4.5 86 5.0 87 3.5 88 2.5 89 2.5 90 1.5 91 1.0 92 0.5 93 0.0 94 1.0 95 1.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5388388597651793 2 0.9155703380383777 3 0.833757108021675 4 0.8218214909104851 5 0.8313699845994371 6 0.8312253104526348 7 0.819434367488247 8 0.8171919182128112 9 0.8205194235892643 10-11 0.8188918394377385 12-13 0.8244979626263277 14-15 0.8245702996997287 16-17 0.8366867594944217 18-19 0.8518775449086635 20-21 0.8512265112480532 22-23 0.8352761865630992 24-25 0.8357102090035061 26-27 0.8373377931550321 28-29 0.849201073192821 30-31 0.8579538590743603 32-33 0.859509106152485 34-35 0.8660194427585888 36-37 0.8695639593552452 38-39 0.8615345442077174 40-41 0.8666704764191991 42-43 0.8612451959141127 44-45 0.8570858141935466 46-47 0.8578815220009592 48-49 0.8598346229827903 50-51 0.9388267071368479 52-53 0.8675023527633123 54-55 0.8717702400939803 56-57 0.89372454187123 58-59 0.8514796910049572 60-61 0.8754594308374392 62-63 0.8831633291546617 64-65 0.8776657115761742 66-67 0.8811378910994295 68-69 0.8952797889493547 70-71 0.9603469864736907 72-73 0.9644340311208557 74-75 0.9676168623505064 76-77 0.9705465138232531 78-79 0.8962563394402702 80-81 0.9021879794591646 82-83 0.9098195407029861 84-85 0.88117405963613 86-87 0.87802739694318 88-89 0.8800890035351127 90-91 0.8900353511277711 92-93 0.8840675425721761 94-95 0.8792932957277001 96-97 0.8835611830583681 98-99 0.9008859121379439 100-101 0.8946287552887443 102-103 0.8786060935303892 104-105 0.8751700825438344 106-107 0.8760019588879477 108-109 0.8767614981586599 110-111 0.8748445657135293 112-113 0.882042104516944 114-115 0.8821506101270455 116-117 0.8946287552887443 118-119 0.8964010135870726 120-121 0.885007924526391 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1382417.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.202647527528946 #Duplication Level Percentage of deduplicated Percentage of total 1 86.25838814235163 54.51758502053832 2 9.226690234780197 11.66302501509012 3 1.9130292752070364 3.627255449722635 4 0.7624900599846307 1.9276556201781208 5 0.4176151099528483 1.3197190298260058 6 0.26047460779067305 0.9877610895639152 7 0.19102160453515615 0.8451149799104949 8 0.1354822511675454 0.6850269573422814 9 0.11088226492389802 0.6307247436335292 >10 0.654128872052659 7.617682248010622 >50 0.04318848325283513 1.8454323697155697 >100 0.020619198261461802 2.3959665571863558 >500 0.0019582351455610172 0.88747248285313 >1k 0.0032253284750416757 4.589594158453172 >5k 3.455709080401795E-4 1.3301166744648634 >10k+ 4.607612107202394E-4 5.129867603510848 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24890 1.800469756954667 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 21279 1.5392605849031082 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14023 1.014382780304351 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10270 0.7429017438298284 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6592 0.47684598786039234 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6569 0.4751822351721659 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5109 0.36957010800648427 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4644 0.33593336887494873 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4605 0.3331122230123038 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4175 0.3020072814498086 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3597 0.26019645302394284 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3269 0.23646989294836507 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3250 0.23509548855374318 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2934 0.21223697335897926 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2660 0.19241661524706366 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2545 0.18409785180593122 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2352 0.17013679663950892 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2342 0.1694134259054974 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2253 0.16297542637279488 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2190 0.15841819074852234 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2181 0.15776715708791197 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2064 0.14930371949997723 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2005 0.14503583216930926 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1962 0.14192533801305973 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1814 0.13121945114968928 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1473 0.10655250911989654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1468 0.10619082375289077 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1385 0.10018684666059517 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 7.233707340115176E-5 0.0 9 0.0 0.0 0.0 7.233707340115176E-5 0.0 10-11 0.0 0.0 0.0 1.446741468023035E-4 0.0 12-13 0.0 0.0 0.0 1.8084268350287936E-4 0.0 14-15 0.0 0.0 0.0 2.1701122020345524E-4 0.0 16-17 0.0 0.0 0.0 2.8934829360460697E-4 0.0 18-19 0.0 3.616853670057588E-5 0.0 6.148651239097899E-4 0.0 20-21 0.0 7.233707340115176E-5 0.0 9.765504909155485E-4 0.0 22-23 0.0 7.233707340115176E-5 0.0 0.0017360897616276422 0.0 24-25 0.0 7.233707340115176E-5 0.0 0.0033275053764529805 0.0 26-27 0.0 7.233707340115176E-5 0.0 0.005642291725289837 0.0 28-29 0.0 7.233707340115176E-5 0.0 0.013924886629721713 0.0 30-31 0.0 7.233707340115176E-5 0.0 0.030779424732190072 0.0 32-33 0.0 7.233707340115176E-5 0.0 0.05635058017949721 0.0 34-35 0.0 7.233707340115176E-5 0.0 0.0794622751311652 0.0 36-37 0.0 1.0850561010172763E-4 0.0 0.10803541912462014 0.0 38-39 0.0 1.446741468023035E-4 0.0 0.150858966578102 0.0 40-41 0.0 2.1701122020345524E-4 0.0 0.2071733782208986 0.0 42-43 0.0 2.1701122020345524E-4 0.0 0.28160822675068375 0.0 44-45 0.0 2.1701122020345524E-4 0.0 0.33202716691128653 0.0 46-47 0.0 2.1701122020345524E-4 0.0 0.4408582938433193 0.0 48-49 0.0 2.89348293604607E-4 0.0 0.5538488024959184 0.0 50-51 0.0 2.89348293604607E-4 0.0 0.5964553387291968 0.0 52-53 0.0 2.89348293604607E-4 0.0 0.6737475016583274 0.0 54-55 0.0 2.89348293604607E-4 0.0 0.8066307054962432 0.0 56-57 0.0 2.89348293604607E-4 0.0 1.0072575785743374 0.0 58-59 0.0 2.89348293604607E-4 0.0 1.2629329645107084 0.0 60-61 0.0 2.89348293604607E-4 0.0 1.538826562462701 0.0 62-63 0.0 2.89348293604607E-4 0.0 1.7688946244150643 0.0 64-65 0.0 2.89348293604607E-4 0.0 2.0168661120342124 0.0 66-67 0.0 2.89348293604607E-4 0.0 2.4885399991464228 0.0 68-69 0.0 2.89348293604607E-4 0.0 2.9845191429214193 0.0 70-71 0.0 2.89348293604607E-4 0.0 3.143588367330552 0.0 72-73 0.0 3.255168303051829E-4 0.0 3.341755779913007 0.0 74-75 0.0 3.978539037063346E-4 0.0 3.5496886974046182 0.0 76-77 0.0 4.340224404069105E-4 0.0 3.7258294711364224 0.0 78-79 0.0 4.340224404069105E-4 0.0 3.939947208403832 0.0 80-81 0.0 4.340224404069105E-4 0.0 4.3038026876116255 0.0 82-83 0.0 4.340224404069105E-4 0.0 4.7949714160054455 0.0 84-85 0.0 4.340224404069105E-4 0.0 5.742297729266929 0.0 86-87 0.0 4.701909771074864E-4 0.0 6.921717542536007 0.0 88-89 0.0 5.063595138080622E-4 0.0 8.135171948840327 0.0 90-91 0.0 5.063595138080622E-4 0.0 9.047921141016062 0.0 92-93 0.0 5.063595138080622E-4 0.0 9.880412350253216 0.0 94-95 0.0 5.063595138080622E-4 0.0 10.691998145277438 0.0 96-97 0.0 5.063595138080622E-4 0.0 11.763780393325604 0.0 98-99 0.0 5.063595138080622E-4 0.0 12.807495856894121 0.0 100-101 0.0 5.063595138080622E-4 0.0 13.454297798710519 0.0 102-103 0.0 5.063595138080622E-4 0.0 14.18971265544333 0.0 104-105 0.0 5.063595138080622E-4 0.0 15.11371026253294 0.0 106-107 0.0 5.063595138080622E-4 0.0 16.083641911232284 0.0 108-109 0.0 5.063595138080622E-4 0.0 17.12525959967217 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1495 0.0 56.550293 2 ACCTGGG 1735 0.0 48.00346 3 TATAACG 240 0.0 47.926693 2 ATAACGC 310 0.0 40.762894 3 GTACCTG 2170 0.0 40.113636 1 GTACACG 180 2.1827873E-11 35.22857 1 GTACCCG 150 5.0640665E-9 34.58805 1 GTATAAC 490 0.0 31.764536 1 TCTAACG 110 2.5735231E-5 31.3702 2 GTACCGG 130 2.2227032E-6 31.040558 1 CTAACGC 130 2.2863096E-6 30.928488 3 TACCCGG 190 1.54796E-9 30.26949 2 TAACGCA 380 0.0 30.231958 4 TACACGG 220 2.3464963E-10 28.756016 2 ACGTGCG 80 0.006375379 28.715122 9 CCTGGGG 1360 0.0 28.298002 4 TACCGCC 280 5.456968E-12 26.665014 8 ACACGGG 235 1.5146725E-8 24.441967 3 GAGTACT 4165 0.0 23.109678 12-13 GTACATG 5425 0.0 21.571041 1 >>END_MODULE