FastQCFastQC Report
Wed 25 May 2016
SRR1003814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4540136
Sequences flagged as poor quality0
Sequence length121
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT427450.9414916205153325No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT292570.6444080089230807No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC188940.4161549345658368No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT167230.3683369837379321No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT117280.25831825302149536No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT107130.23596209452756484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101740.22409020346527064No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC88530.19499415876528808No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC81710.17997258231911994No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG77770.17129442818453017No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC77360.17039137153600686No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75680.16669104185425282No Hit
GTACAAGCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCAGT72140.15889391859627114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71470.15741819187795256No Hit
GAGTACAAGCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCA71190.1568014702643269No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA59550.13116347175503112No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA58830.12957761617713653No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56530.12451168863663997No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA56050.12345445158471025No Hit
GCTTACTGCTTGTACTCTGCGTTGATACCACTGCTTACTGCTTGTACTCT55800.12290380728683017No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA48490.10680296801681712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47570.10477659700061848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG35050.031.3438032
TATAACG7750.028.2025432
ACCTGGG39750.026.1639583
TAACGCA8550.025.5358624
GTACATG190250.025.2408471
GTACACG7900.024.8104931
TACATGG188300.023.7037872
ACATGGG190200.022.5367013
GTATAAC12950.022.2578831
GAGTACT128900.021.93021212-13
AGTACTT136100.021.31885512-13
CATGGGT38850.021.1484284
TACACGG7250.020.6272662
CATGGGG78250.020.2655224
GTACTTT141850.020.1522414-15
GTACTAG14550.019.810281
GTACCTG63100.019.1855181
GAGTACG12900.018.3222051
GTATACG13000.018.1812651
GTATTAG27200.017.8030321
Produced by FastQC (version 0.11.5)