##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003814_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4540136 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.702541069254313 31.0 31.0 34.0 27.0 34.0 2 30.62749287686536 31.0 31.0 34.0 27.0 34.0 3 30.693314253141317 31.0 31.0 34.0 27.0 34.0 4 34.18139522692712 37.0 35.0 37.0 30.0 37.0 5 34.13249250683239 37.0 35.0 37.0 30.0 37.0 6 34.25152440367425 37.0 35.0 37.0 30.0 37.0 7 34.270212830628864 37.0 35.0 37.0 30.0 37.0 8 34.24258612517334 37.0 35.0 37.0 30.0 37.0 9 35.67658589962944 39.0 35.0 39.0 30.0 39.0 10-11 35.515156484299155 38.0 35.0 39.0 30.0 39.0 12-13 35.360100886845686 38.0 35.0 39.0 29.5 39.0 14-15 36.308711236844005 39.0 36.0 41.0 29.0 41.0 16-17 36.38127040687768 39.0 36.0 41.0 29.5 41.0 18-19 36.34165044835661 39.0 36.0 41.0 29.5 41.0 20-21 36.21761187330071 39.0 36.0 41.0 28.0 41.0 22-23 36.13589306575838 39.0 35.5 40.5 27.5 41.0 24-25 35.95295823737439 39.0 35.0 41.0 27.0 41.0 26-27 35.75845492293623 39.0 35.0 40.0 27.0 41.0 28-29 35.57089798631583 38.0 35.0 40.0 27.0 41.0 30-31 35.36746057827342 38.0 34.0 40.0 26.5 41.0 32-33 35.11557572284178 38.0 34.0 40.0 25.5 41.0 34-35 35.10461624938108 38.0 34.0 40.0 25.0 41.0 36-37 34.98471367377542 38.0 34.0 40.0 25.0 41.0 38-39 35.01720895585507 38.0 34.0 40.0 25.0 41.0 40-41 35.105274049059325 38.0 34.0 40.0 25.0 41.0 42-43 34.95948744707207 38.0 34.0 40.0 24.5 41.0 44-45 34.73674918548696 38.0 34.0 40.0 24.0 41.0 46-47 34.46586214597977 38.0 33.5 40.0 23.0 41.0 48-49 34.21462121399007 38.0 33.0 40.0 22.5 41.0 50-51 33.45857668140337 37.5 32.5 39.5 21.0 40.5 52-53 33.616137710412204 37.5 33.0 39.5 22.0 40.5 54-55 33.95249338345812 38.0 33.0 40.0 22.0 41.0 56-57 33.77990328924068 38.0 33.0 40.0 20.5 41.0 58-59 33.51981317299746 38.0 33.0 40.0 19.0 41.0 60-61 33.23192719337042 37.0 32.0 40.0 18.0 41.0 62-63 32.9820792813255 37.0 32.0 40.0 18.0 41.0 64-65 32.676629290400115 36.5 31.5 39.5 15.0 41.0 66-67 32.31128693061177 36.0 31.0 39.0 13.0 41.0 68-69 31.929509600593462 35.5 31.0 39.0 11.5 40.5 70-71 31.527116037933666 35.0 31.0 38.5 8.0 40.0 72-73 31.160913461623174 35.0 30.0 37.5 7.5 40.0 74-75 30.777836060417574 35.0 30.0 37.0 7.0 39.0 76-77 30.34025919047359 34.5 29.5 36.5 4.0 39.0 78-79 29.784723739553176 34.0 29.0 36.0 2.0 39.0 80-81 29.392024159628697 34.0 29.0 36.0 2.0 37.5 82-83 29.049585188637522 34.0 29.0 35.0 2.0 37.0 84-85 28.798742372475186 34.0 29.0 35.0 2.0 37.0 86-87 28.568297843941238 34.0 28.5 35.0 2.0 36.0 88-89 28.24472460736859 34.0 27.5 35.0 2.0 36.0 90-91 27.89910731749005 34.0 26.5 35.0 2.0 35.5 92-93 27.63144408008923 34.0 26.0 35.0 2.0 35.0 94-95 27.376918334604955 33.5 26.0 35.0 2.0 35.0 96-97 27.062335247226073 33.0 25.0 35.0 2.0 35.0 98-99 26.787407249474466 33.0 25.0 35.0 2.0 35.0 100-101 26.477753750107926 33.0 25.0 35.0 2.0 35.0 102-103 26.17103221136988 32.5 24.0 35.0 2.0 35.0 104-105 25.86430637760631 32.0 23.0 35.0 2.0 35.0 106-107 25.527911498686382 32.0 20.0 35.0 2.0 35.0 108-109 25.187535681750504 32.0 19.0 34.5 2.0 35.0 110-111 24.789136162440947 32.0 18.0 34.0 2.0 35.0 112-113 24.31102922908036 31.5 11.5 34.0 2.0 35.0 114-115 23.795062306503596 31.0 4.5 34.0 2.0 35.0 116-117 23.13656132767829 31.0 2.0 34.0 2.0 35.0 118-119 22.356863318631866 31.0 2.0 34.0 2.0 35.0 120-121 20.681697971162098 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3756265705976425 1101 2 0.37148357270293175 1101 3 0.46420144784842066 1101 4 0.629120758753146 1101 5 0.6468110690579394 1101 6 0.7154183830152405 1101 7 0.5057314139157754 1101 8 0.5671034769946246 1101 9 0.667715037386138 1101 10-11 0.6935025233717269 1101 12-13 0.7829632001760771 1101 14-15 0.9626089223077443 1101 16-17 0.772983886571943 1101 18-19 0.6727541557722603 1101 20-21 0.7724256791562141 1101 22-23 0.6844599200110864 1101 24-25 0.6935222208392915 1101 26-27 0.7624311523563918 1101 28-29 0.7219050053925926 1101 30-31 0.7040194764171517 1101 32-33 0.7188199824699737 1101 34-35 0.8315826857288258 1101 36-37 0.7409173234133846 1101 38-39 0.5706336251634596 1101 40-41 0.5395300500853608 1101 42-43 0.5685605538002392 1101 44-45 0.6830439085536995 1101 46-47 0.6442851025558056 1101 48-49 0.7693250757170986 1101 50-51 0.5484123101994172 1101 52-53 0.4912286187212942 1101 54-55 0.6678877922643025 1101 56-57 0.7229305427676991 1101 58-59 0.7229752449617237 1101 60-61 0.6784858564836469 1101 62-63 0.5987577220698626 1101 64-65 0.690470194701426 1101 66-67 0.6753108040787978 1101 68-69 0.5672315051140622 1101 70-71 0.3950156445229567 1101 72-73 0.41170605784074255 1101 74-75 0.40498684304688837 1101 76-77 0.38997011715438745 1101 78-79 0.6351607560828754 1101 80-81 0.6173152976399905 1101 82-83 0.7382853547971315 1101 84-85 0.6701997354201374 1101 86-87 0.592123588990809 1101 88-89 0.6579018822720748 1101 90-91 0.6230996373819764 1101 92-93 0.6400478265441301 1101 94-95 0.8280524416691009 1101 96-97 0.6959445834001663 1101 98-99 0.6561826966243132 1101 100-101 0.5972501743259571 1101 102-103 0.461454812553324 1101 104-105 0.5404581597567564 1101 106-107 0.36882780526252645 1101 108-109 0.49116219667829597 1101 110-111 0.4674393439148332 1101 112-113 0.4532347458048953 1101 114-115 0.37624795857010085 1101 116-117 0.1671670281107147 1101 118-119 0.27553753344295373 1101 120-121 0.31412617430860834 1102 1 0.29858670397122467 1102 2 0.41327525375684715 1102 3 0.4688056940535148 1102 4 0.661214138938135 1102 5 0.7294156547203698 1102 6 0.6829852186401411 1102 7 0.5216997766784743 1102 8 0.5534728873590922 1102 9 0.5845052184308344 1102 10-11 0.6531716295086483 1102 12-13 0.5843322124836234 1102 14-15 0.7478270026367895 1102 16-17 0.6407416494406775 1102 18-19 0.5249462587431637 1102 20-21 0.6937795069346038 1102 22-23 0.6304655647150454 1102 24-25 0.751045975265491 1102 26-27 0.7102637265800027 1102 28-29 0.7531046835851569 1102 30-31 0.6925726900906071 1102 32-33 0.4707846677003289 1102 34-35 0.5412374454740387 1102 36-37 0.5028891830069213 1102 38-39 0.5281087255940449 1102 40-41 0.5526951816064098 1102 42-43 0.6733887858181618 1102 44-45 0.7323957294715058 1102 46-47 0.7683516240542687 1102 48-49 0.5258392363878173 1102 50-51 0.3038423319092729 1102 52-53 0.4830222258570913 1102 54-55 0.48652682514240553 1102 56-57 0.4945302669994689 1102 58-59 0.4903308886267652 1102 60-61 0.5532963008435701 1102 62-63 0.44613384990753246 1102 64-65 0.3446896797861996 1102 66-67 0.373951107003613 1102 68-69 0.21837037981998364 1102 70-71 0.16587563658049476 1102 72-73 0.21444081395324943 1102 74-75 0.29036043405962886 1102 76-77 0.14924604577682743 1102 78-79 0.29417230953911755 1102 80-81 0.4012553701120325 1102 82-83 0.41541454492989516 1102 84-85 0.34732883216711485 1102 86-87 0.4015204638821004 1102 88-89 0.3463236554487388 1102 90-91 0.48205091031197966 1102 92-93 0.44204184658518386 1102 94-95 0.27458107013304556 1102 96-97 0.3430957448742369 1102 98-99 0.4351379524581809 1102 100-101 0.4065199745060539 1102 102-103 0.5181361571312415 1102 104-105 0.533379143473308 1102 106-107 0.3947738791722415 1102 108-109 0.432623525506699 1102 110-111 0.36917711335325976 1102 112-113 0.2273250690061026 1102 114-115 0.06046403273896317 1102 116-117 0.30234510430277695 1102 118-119 0.21951822406353827 1102 120-121 0.2667872373610578 1103 1 0.5150374670661293 1103 2 0.6410710894670331 1103 3 0.7062077314360735 1103 4 0.9009429549044228 1103 5 0.9087452845650077 1103 6 0.8686984008881566 1103 7 0.7318409196104625 1103 8 0.759529142069816 1103 9 0.7207603225620645 1103 10-11 0.871707632014953 1103 12-13 0.8738023615619213 1103 14-15 0.9877872346717638 1103 16-17 0.9230216602450483 1103 18-19 0.7856507451971524 1103 20-21 1.0246801848946916 1103 22-23 0.9783919356879309 1103 24-25 0.9325537275958382 1103 26-27 1.0644192126544922 1103 28-29 0.9954174097657571 1103 30-31 1.0262093407154325 1103 32-33 1.035182335836211 1103 34-35 0.9385611799301614 1103 36-37 0.7855671613223691 1103 38-39 0.8171342977053158 1103 40-41 0.9117631556087389 1103 42-43 0.9617817359160625 1103 44-45 0.9018633213866494 1103 46-47 0.8965682882181412 1103 48-49 1.039345687587499 1103 50-51 0.776216286075126 1103 52-53 0.8666755004412394 1103 54-55 0.8537347819978365 1103 56-57 0.8658688579497991 1103 58-59 0.8383352938560478 1103 60-61 0.9178151823509637 1103 62-63 0.9038942163734802 1103 64-65 0.8495291230442845 1103 66-67 0.8341515012769136 1103 68-69 0.693461739631072 1103 70-71 0.6412176031780916 1103 72-73 0.5954591070252597 1103 74-75 0.45195181953830144 1103 76-77 0.47884844768707424 1103 78-79 0.5736755674033525 1103 80-81 0.5438048617739035 1103 82-83 0.5910454277844757 1103 84-85 0.6775474000502015 1103 86-87 0.6393158150266594 1103 88-89 0.7432202525026739 1103 90-91 0.6349154128094163 1103 92-93 0.6049666990925253 1103 94-95 0.6267937838876563 1103 96-97 0.4794701193168045 1103 98-99 0.5735013488506127 1103 100-101 0.47951762700149203 1103 102-103 0.4405918397666646 1103 104-105 0.6242779215523306 1103 106-107 0.5036059992524038 1103 108-109 0.6354176220580783 1103 110-111 0.6797725965299577 1103 112-113 0.558786307834108 1103 114-115 0.5824958164058387 1103 116-117 0.5392036125794952 1103 118-119 0.4470566642707787 1103 120-121 0.6559034376373098 1104 1 0.4084083713046667 1104 2 0.6100819949196072 1104 3 0.6599940808566487 1104 4 0.8544234308643297 1104 5 0.9749048631460724 1104 6 0.7852840134515233 1104 7 0.6402529503559009 1104 8 0.6584850498667905 1104 9 0.7652032636408137 1104 10-11 0.747879772681955 1104 12-13 0.8718730422528154 1104 14-15 1.0404233060500516 1104 16-17 0.9738371699489008 1104 18-19 0.8452245656058466 1104 20-21 0.8381905305562896 1104 22-23 0.7572033010191248 1104 24-25 0.9126917375121906 1104 26-27 1.0671998246075347 1104 28-29 0.9279758809998029 1104 30-31 0.8742479779702137 1104 32-33 0.9146167564244152 1104 34-35 0.7557646521565147 1104 36-37 0.7429428672542073 1104 38-39 0.647679242241054 1104 40-41 0.7663292601584715 1104 42-43 0.8042261508605009 1104 44-45 0.7679328918561623 1104 46-47 0.8254040856056761 1104 48-49 0.8590749087334402 1104 50-51 0.6169818244705638 1104 52-53 0.7792966478420809 1104 54-55 0.7827831070735272 1104 56-57 0.7647493374459273 1104 58-59 0.7726802646581277 1104 60-61 0.6470985194143495 1104 62-63 0.7619706325463298 1104 64-65 0.8110444031492037 1104 66-67 0.7576802286570512 1104 68-69 0.7789266138698032 1104 70-71 0.7510976799802194 1104 72-73 0.6034320746190289 1104 74-75 0.7458569055379094 1104 76-77 0.7257925732572517 1104 78-79 0.7213172830215946 1104 80-81 0.8987505661617199 1104 82-83 0.8976210973329692 1104 84-85 0.8445470291254473 1104 86-87 0.9424965019021556 1104 88-89 0.9287885044070094 1104 90-91 1.0147830438237158 1104 92-93 1.0175176421519332 1104 94-95 1.0291380321286923 1104 96-97 0.961915488283875 1104 98-99 1.0294856851037864 1104 100-101 0.996045241796466 1104 102-103 1.0228153535596682 1104 104-105 1.0711829454317332 1104 106-107 1.046850809875128 1104 108-109 0.9195724832856058 1104 110-111 0.8576887406824909 1104 112-113 0.7921525592030925 1104 114-115 0.6640826453717033 1104 116-117 0.632101495566399 1104 118-119 0.5887506302620018 1104 120-121 0.7893962364683524 1105 1 0.719533715952533 1105 2 0.8057055777436801 1105 3 0.8139900756975571 1105 4 1.0566527585584709 1105 5 1.0219649176571295 1105 6 0.9946915340117854 1105 7 0.9250811298215922 1105 8 0.9670869070609243 1105 9 1.0254112370760637 1105 10-11 1.053686745025118 1105 12-13 1.1679614601923802 1105 14-15 1.3632499452437372 1105 16-17 1.155520076685626 1105 18-19 1.0206760638409165 1105 20-21 1.2367684618529253 1105 22-23 1.1258792262625619 1105 24-25 1.2727984523243592 1105 26-27 1.1984109347866934 1105 28-29 1.274783982141507 1105 30-31 1.2199912467640601 1105 32-33 1.2226338101218488 1105 34-35 1.0691412396265463 1105 36-37 1.162196705155118 1105 38-39 1.2463623435592623 1105 40-41 1.2361056580739245 1105 42-43 1.2022596634968608 1105 44-45 1.1779919073755138 1105 46-47 1.2168735090598872 1105 48-49 1.146011783845175 1105 50-51 1.1021752042944755 1105 52-53 1.1966899591529696 1105 54-55 1.2150763049397355 1105 56-57 1.1405060942103091 1105 58-59 1.2426025986870428 1105 60-61 1.2616335868669069 1105 62-63 1.2372830007837408 1105 64-65 1.1693945893854263 1105 66-67 1.1077013953947414 1105 68-69 1.0544780716353124 1105 70-71 0.8125488568310715 1105 72-73 0.9141166456063949 1105 74-75 0.7868270396805208 1105 76-77 0.8201922696084658 1105 78-79 1.0495650922854232 1105 80-81 1.00828991638382 1105 82-83 1.013384725167782 1105 84-85 0.8410578357405392 1105 86-87 0.8026645706004345 1105 88-89 0.8399080454997083 1105 90-91 0.879801387859029 1105 92-93 0.9463460752231043 1105 94-95 0.9244093490386724 1105 96-97 0.8108771178882783 1105 98-99 0.9134559550863024 1105 100-101 0.9952926152994372 1105 102-103 1.0122239359869525 1105 104-105 1.0665636120526472 1105 106-107 1.1395219363833107 1105 108-109 1.21618618651015 1105 110-111 1.053038172597752 1105 112-113 1.2083252882550113 1105 114-115 1.2101039199905692 1105 116-117 1.2381774930506744 1105 118-119 1.2318384124934063 1105 120-121 1.2386080684911533 1106 1 0.5047746748572912 1106 2 0.723011979477338 1106 3 0.609408340367029 1106 4 0.8021199186083621 1106 5 0.8814642207900079 1106 6 0.8765389185801951 1106 7 0.7482735120411093 1106 8 0.8461752847065398 1106 9 0.9003465949780889 1106 10-11 1.0032238894958994 1106 12-13 1.0190939238184598 1106 14-15 0.9717338950135854 1106 16-17 0.9632856901260212 1106 18-19 0.8550590390553978 1106 20-21 0.9005181901216375 1106 22-23 0.7376617221899551 1106 24-25 1.0125973744252121 1106 26-27 1.0188432189795904 1106 28-29 1.0388084271387825 1106 30-31 0.9925459879073415 1106 32-33 1.048962140813778 1106 34-35 0.858399874970452 1106 36-37 0.9110873543581803 1106 38-39 0.9210483215564835 1106 40-41 0.9288352896959253 1106 42-43 0.9330060221583807 1106 44-45 0.8132883175696435 1106 46-47 0.8229021037803719 1106 48-49 0.909457990244313 1106 50-51 0.6664077204699694 1106 52-53 0.6440622839869334 1106 54-55 0.8550481414606352 1106 56-57 0.8431362858796518 1106 58-59 0.9023882074640142 1106 60-61 0.7729677876974321 1106 62-63 0.8438432880774158 1106 64-65 0.7501542873716076 1106 66-67 0.6884172170781113 1106 68-69 0.5820031621481796 1106 70-71 0.4467777496132612 1106 72-73 0.5689310692746723 1106 74-75 0.6225198037863819 1106 76-77 0.7052156020512754 1106 78-79 0.8014885690418012 1106 80-81 0.6317095453509474 1106 82-83 0.6567610377772901 1106 84-85 0.6144210383893487 1106 86-87 0.6924684699919297 1106 88-89 0.6348407225364134 1106 90-91 0.5451766054646008 1106 92-93 0.5242505402149682 1106 94-95 0.6014022919648383 1106 96-97 0.7084657180726417 1106 98-99 0.8130264409042276 1106 100-101 0.8169131115594581 1106 102-103 0.6483969900682816 1106 104-105 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74-75 1.3693403448784487 2206 76-77 1.3683791886607324 2206 78-79 1.460569282028768 2206 80-81 1.4871231973159702 2206 82-83 1.4577903026019534 2206 84-85 1.4570182117123132 2206 86-87 1.3502032769361385 2206 88-89 1.4073233017011866 2206 90-91 1.434906522771925 2206 92-93 1.5822923801334987 2206 94-95 1.6809204060242848 2206 96-97 1.618867341036701 2206 98-99 1.6791947009244375 2206 100-101 1.64582475603558 2206 102-103 1.4594601430549297 2206 104-105 1.6018395597078978 2206 106-107 1.6602083112694395 2206 108-109 1.7025074202086792 2206 110-111 1.653186281519659 2206 112-113 1.7644289428344706 2206 114-115 1.8120899752294797 2206 116-117 1.799507241941516 2206 118-119 1.7461845380977046 2206 120-121 1.613885232122584 2207 1 0.663895493053186 2207 2 0.971875636348507 2207 3 0.9567577058877497 2207 4 1.151908852969818 2207 5 1.136581599290892 2207 6 1.1671649272473346 2207 7 1.0761870884176972 2207 8 1.127353357640871 2207 9 1.196768923170005 2207 10-11 1.3278581246967747 2207 12-13 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1.3781354641503896 2208 86-87 1.387068741636341 2208 88-89 1.3646776066465662 2208 90-91 1.5311232800582495 2208 92-93 1.4543896436803898 2208 94-95 1.4911943688292908 2208 96-97 1.4978999041029581 2208 98-99 1.4923718014117746 2208 100-101 1.4667072370417387 2208 102-103 1.5367380783781748 2208 104-105 1.5841338198272474 2208 106-107 1.6315310977401296 2208 108-109 1.6073194669940989 2208 110-111 1.7439605736815267 2208 112-113 1.712548276779998 2208 114-115 1.7075095772659097 2208 116-117 1.6806821074076126 2208 118-119 1.614799847190742 2208 120-121 1.3790676588406825 2209 1 0.5733340034987364 2209 2 0.766098743465399 2209 3 0.9096783214240318 2209 4 0.987865367912967 2209 5 1.0822534726247 2209 6 0.9753739482262347 2209 7 0.883264431756345 2209 8 0.8359061772315783 2209 9 0.9859337315001824 2209 10-11 1.045464026126794 2209 12-13 1.1184727612172978 2209 14-15 1.282003593222484 2209 16-17 1.1815298974223012 2209 18-19 1.1488095669505043 2209 20-21 1.2947659790651613 2209 22-23 1.2446056626740258 2209 24-25 1.3319320413567013 2209 26-27 1.3263800489909698 2209 28-29 1.2929088541768223 2209 30-31 1.3298035811172326 2209 32-33 1.3784670357486206 2209 34-35 1.3667308274373795 2209 36-37 1.3277640980590277 2209 38-39 1.3779170960374714 2209 40-41 1.4423314084370418 2209 42-43 1.4672614898606327 2209 44-45 1.436838657470517 2209 46-47 1.448846441855764 2209 48-49 1.5291283622732905 2209 50-51 1.4162809767436926 2209 52-53 1.3750771809275264 2209 54-55 1.325043591244473 2209 56-57 1.2793577943613954 2209 58-59 1.2046084124972012 2209 60-61 1.2083690758025583 2209 62-63 1.2324191705703456 2209 64-65 1.3471024111126084 2209 66-67 1.362243924613125 2209 68-69 1.262286239502675 2209 70-71 1.4119049453879313 2209 72-73 1.444956711509061 2209 74-75 1.4040522325560936 2209 76-77 1.255502375991835 2209 78-79 1.0673441514950959 2209 80-81 1.20205118869373 2209 82-83 1.269614125025214 2209 84-85 1.3748706229058278 2209 86-87 1.2966272642812768 2209 88-89 1.50035212227343 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1.174739446593101 2210 96-97 1.2940231532995483 2210 98-99 1.192724657660495 2210 100-101 1.2583137849018513 2210 102-103 1.2799291756447424 2210 104-105 1.3201928257811595 2210 106-107 1.284912423117376 2210 108-109 1.4670774182582562 2210 110-111 1.5952559249467342 2210 112-113 1.6917075032444124 2210 114-115 1.7700140150890498 2210 116-117 1.6558003507396997 2210 118-119 1.6501394655774 2210 120-121 1.4903330893161026 2211 1 0.3070055403863847 2211 2 0.5599714783546723 2211 3 0.5349972183034879 2211 4 0.6153670415322807 2211 5 0.7870794902997318 2211 6 0.661397846297767 2211 7 0.5681064582567714 2211 8 0.5278136048685838 2211 9 0.6212867338452668 2211 10-11 0.6093274534028126 2211 12-13 0.7491201993337953 2211 14-15 0.8180590947492803 2211 16-17 0.8379028343581751 2211 18-19 0.7639200426624839 2211 20-21 0.7139593619044007 2211 22-23 0.6941746804446964 2211 24-25 0.8462820473507549 2211 26-27 0.908561492223285 2211 28-29 0.9018131824190263 2211 30-31 0.8681695865078325 2211 32-33 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-1.0817045921469344 2316 72-73 -1.1477358994009244 2316 74-75 -0.9690427633665983 2316 76-77 -0.9989380249629534 2316 78-79 -0.9777044756962461 2316 80-81 -0.9663940349667719 2316 82-83 -0.8993282182012869 2316 84-85 -0.8930987894233162 2316 86-87 -0.7547652801396652 2316 88-89 -0.5565538794782583 2316 90-91 -0.658625922272325 2316 92-93 -0.8484730674747638 2316 94-95 -0.8291589532783803 2316 96-97 -0.6052386930772009 2316 98-99 -0.47034185876363566 2316 100-101 -0.2779670700105328 2316 102-103 -0.3071377719562811 2316 104-105 -0.4905198077018085 2316 106-107 -0.5788463822877148 2316 108-109 -0.5046157050386313 2316 110-111 -0.5979674936419173 2316 112-113 -0.5384073042339672 2316 114-115 -0.2772851996991825 2316 116-117 -0.2487721135006531 2316 118-119 -0.29021987565402085 2316 120-121 -0.7958359456660631 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59824.0 3 9387.0 4 8553.0 5 9864.0 6 11780.0 7 12490.0 8 15214.0 9 17994.0 10 22785.0 11 27653.0 12 30341.0 13 35658.0 14 43723.0 15 42751.0 16 33297.0 17 32643.0 18 34655.0 19 37580.0 20 41328.0 21 45687.0 22 51260.0 23 57846.0 24 65315.0 25 74581.0 26 86062.0 27 100136.0 28 117068.0 29 137283.0 30 161965.0 31 193535.0 32 233854.0 33 288736.0 34 368203.0 35 478047.0 36 634069.0 37 687243.0 38 230846.0 39 880.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.50654953819046 19.281507684731004 14.05092593104727 22.161016846031263 2 14.290110256339045 20.67262476740634 40.575821368067324 24.4614436081873 3 20.543631365617937 25.50115780424053 29.68147439887173 24.273736431269803 4 12.000269246635916 15.899458152252752 40.187570713341415 31.912701887769916 5 12.024646846871665 38.59267961809562 35.57989718779424 13.80277634723847 6 29.108239721346024 37.67117017428317 17.78916616227737 15.431423942093442 7 26.897337791524755 32.81311227196271 22.681552381824012 17.60799755468852 8 24.52738074322381 34.08884595676289 20.263240369090912 21.12053293092239 9 24.22867735312487 18.660264445863938 22.734050936434734 34.377007264576456 10-11 24.35726302854816 26.037019899531277 28.246958638685378 21.358758433235185 12-13 26.251467732754598 23.91964097929839 28.134063326102847 21.69482796184417 14-15 22.323958222982068 25.866192192316245 27.64631495067257 24.163534634029123 16-17 20.71304071973189 29.14682678085792 28.286049352053805 21.854083147356388 18-19 20.39445862826583 29.61357463187558 29.743151229066576 20.248815510792014 20-21 21.776660252822985 27.30836433876249 28.77952653990298 22.13544886851154 22-23 20.896483887334405 27.265656168946855 29.371993897383998 22.465866046334742 24-25 21.794100641858446 27.607220924181096 29.134636122879122 21.46404231108134 26-27 21.401279756731828 26.782022100042703 30.4524239356601 21.36427420756537 28-29 20.370221428998327 28.543584939993128 29.699009153944957 21.38718447706359 30-31 21.15241792214109 27.53895906302941 29.15554728997168 22.15307572485782 32-33 21.51307489989811 27.68630993615963 29.487888459988348 21.312726703953906 34-35 22.082387985677045 26.943899761984042 29.766904793727832 21.206807458611078 36-37 21.624330128439112 26.854520158676877 30.558765857381708 20.962383855502306 38-39 21.371110154917766 28.12443860211051 29.853163587164893 20.651287655806836 40-41 20.992502796618194 28.091968628099334 29.52968815207203 21.385840423210443 42-43 20.63419812539198 28.240231579029235 28.707333724779367 22.418236570799422 44-45 21.37765875258223 27.553786000582736 28.6512835648404 22.417271681994634 46-47 21.99210434123499 28.04549456276529 28.69793829159022 21.2644628044095 48-49 21.965555039157856 27.912501544009377 28.72612783775594 21.395815579076828 50-51 20.68544451319032 28.427444804767642 29.682671861721232 21.204438820320807 52-53 21.060630196082876 28.862211007203335 28.14647596691214 21.930682829801647 54-55 20.62376346742842 28.28512152459911 28.648271031941423 22.442843976031043 56-57 21.65683917238074 27.66285782948413 28.456467777686505 22.223835220448628 58-59 22.484594775024156 27.24411370176089 28.349936396777437 21.92135512643752 60-61 21.457606003744008 27.69037999596594 29.71619907350041 21.13581492678964 62-63 20.94570401319902 28.237061580067618 29.14952140658327 21.66771300015009 64-65 20.7683301396873 29.257552278597014 28.492910105806878 21.4812074759088 66-67 20.95531172836626 27.78155841743683 28.383990942461256 22.87913891173565 68-69 20.623428274427813 28.37487825619541 28.868642017995082 22.133051451381696 70-71 21.612471943582392 28.134782802411824 28.18015972811177 22.072585525894013 72-73 21.33504229058051 27.311025774364307 29.271804205501557 22.082127729553626 74-75 20.739921911712923 28.867197574567182 28.69858447895009 21.69429603476981 76-77 21.241364824495893 27.792140100999323 28.499202051993315 22.46729302251147 78-79 21.47207286934963 27.98343429549216 28.442426325685062 22.102066509473147 80-81 21.851228001167573 27.7525794342954 28.181498821745382 22.214693742791646 82-83 21.36019396553162 27.452274217836266 29.6185184419152 21.569013374716917 84-85 21.26720988417263 27.892744367295357 28.80757051509335 22.032475233438664 86-87 21.320578446079306 28.412458151359978 28.061266842386413 22.205696560174307 88-89 21.07510977736711 27.85446435367898 27.878900687251047 23.191525181702865 90-91 21.155460450075793 26.65715982332875 28.796421852669713 23.390957873925743 92-93 21.689341788795105 27.44228356141063 28.555707770066796 22.312666879727473 94-95 21.221861164484544 26.999925773522126 29.097212684638365 22.68100037735497 96-97 20.096028815312337 27.4135196685599 30.124423393149275 22.36602812297849 98-99 20.56136511844712 28.312605151877833 28.723507618737827 22.402522110937216 100-101 20.280147794645874 27.42292951165543 29.062993905321573 23.233928788377128 102-103 20.69074456730398 26.91573549683659 28.94277521298235 23.45074472287708 104-105 21.38847527653694 26.876669518897444 28.960031324538416 22.774823880027196 106-107 21.04573908479163 27.120124126182212 29.470101470309473 22.364035318716684 108-109 21.105177666847148 26.784721232255176 29.086305262244444 23.02379583865323 110-111 20.57228811581005 27.621418699334686 28.48556302101406 23.3207301638412 112-113 21.493433781148855 26.667734913687013 27.69575228462812 24.143079020536014 114-115 20.980115674176513 26.74105504263467 28.30493559377333 23.973893689415494 116-117 21.882988084318157 26.285285406791214 27.698791446888983 24.13293506200165 118-119 21.747506491020722 26.13504635443002 28.361495083897253 23.755952070652008 120-121 21.729885809160983 27.837910549564874 27.352094698864292 23.08010894240985 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10751.0 1 11708.0 2 7451.5 3 2162.0 4 2141.0 5 2176.0 6 2181.5 7 2421.5 8 2762.5 9 2890.5 10 2863.5 11 2904.0 12 3102.0 13 3428.5 14 3767.0 15 4107.5 16 4525.5 17 5092.5 18 5686.5 19 6225.0 20 6920.0 21 7890.0 22 9241.5 23 11209.0 24 13732.0 25 16967.0 26 21273.0 27 26901.5 28 34712.0 29 43976.5 30 53351.5 31 62976.0 32 74110.5 33 85043.5 34 94888.0 35 106676.0 36 118844.0 37 133145.5 38 145755.0 39 151364.5 40 156582.5 41 162534.0 42 167962.0 43 175530.0 44 179986.0 45 184427.0 46 198767.0 47 245505.0 48 317509.0 49 295367.0 50 212900.0 51 170275.5 52 140036.0 53 118177.0 54 102127.0 55 87276.5 56 72676.5 57 58465.0 58 46420.0 59 38055.0 60 30712.0 61 23732.5 62 17664.0 63 12361.5 64 8295.0 65 5475.5 66 3432.0 67 2095.0 68 1265.0 69 771.0 70 489.0 71 333.5 72 225.5 73 159.0 74 120.5 75 86.5 76 72.5 77 58.5 78 44.5 79 39.0 80 29.5 81 24.0 82 21.5 83 15.5 84 11.0 85 6.5 86 9.0 87 10.0 88 7.5 89 8.5 90 7.0 91 3.0 92 2.5 93 2.5 94 2.5 95 2.5 96 4.0 97 4.0 98 1.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4448985669151761 2 0.8647538311627669 3 0.8767799026284675 4 0.8521110380834408 5 0.8615160426912322 6 0.8731897018062895 7 0.8471111878586898 8 0.8356357606908692 9 0.8312526320797438 10-11 0.8224753619715357 12-13 0.8247109778209287 14-15 0.825283647890724 16-17 0.8520009092238646 18-19 0.8831233249400458 20-21 0.8747425187263113 22-23 0.8471111878586898 24-25 0.8381466986892022 26-27 0.8393691290304961 28-29 0.8641811610929716 30-31 0.8710421890445572 32-33 0.863894826058074 34-35 0.8767909155144251 36-37 0.8876275952967049 38-39 0.8733548950956534 40-41 0.8823083713791834 42-43 0.8750508795331241 44-45 0.8577165970358597 46-47 0.8518467288204582 48-49 0.8560977028000923 50-51 0.8914270409520771 52-53 0.85964385207844 54-55 0.8732778048939503 56-57 0.9241242993601955 58-59 0.8890482575852353 60-61 0.9018121924100952 62-63 0.8700069777645427 64-65 0.8592473881839664 66-67 0.8619565581295362 68-69 0.8789824798199878 70-71 0.8821652038617345 72-73 0.8879910205333056 74-75 0.8917243888729325 76-77 0.9051711226271636 78-79 0.9686824359446501 80-81 0.9989128078982655 82-83 0.9617993822211494 84-85 0.9169549106017969 86-87 0.8972528576236483 88-89 0.8961956205717186 90-91 0.9090366455982817 92-93 0.8950833190900007 94-95 0.8897090307426914 96-97 0.9000060791130486 98-99 0.935544662098228 100-101 0.9221640056597424 102-103 0.8952044608355345 104-105 0.8843237295094244 106-107 0.885414005219227 108-109 0.8838171367553747 110-111 0.8827378739315298 112-113 0.893200115591251 114-115 0.8961735947998033 116-117 0.9257431935959628 118-119 0.9208204335729151 120-121 0.8963277752032098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 4540136.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.50206543547991 #Duplication Level Percentage of deduplicated Percentage of total 1 75.92671685148672 37.58529305883451 2 14.011449852676487 13.871914149062736 3 4.235265103815193 6.28963110916993 4 1.8534912812216402 3.670065867485006 5 0.9914544354924297 2.453952117102153 6 0.6127338132603602 1.819895359112729 7 0.4203260968110492 1.45649069640063 8 0.30068469860208935 1.1907610900518546 9 0.2201165294623633 0.9806600560388987 >10 1.2471793793381432 11.623053540524197 >50 0.11047330538055661 3.7677684812199597 >100 0.062344757150342774 5.6567534685518375 >500 0.003927086738678796 1.3369281663948063 >1k 0.0029340300782015735 3.146491251820175 >5k 5.868060156402978E-4 2.0168549517138463 >10k+ 3.1597246996016034E-4 3.133486636516763 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 42745 0.9414916205153325 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 29257 0.6444080089230807 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18894 0.4161549345658368 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 16723 0.3683369837379321 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11728 0.25831825302149536 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10713 0.23596209452756484 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10174 0.22409020346527064 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8853 0.19499415876528808 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8171 0.17997258231911994 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7777 0.17129442818453017 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7736 0.17039137153600686 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7568 0.16669104185425282 No Hit GTACAAGCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCAGT 7214 0.15889391859627114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7147 0.15741819187795256 No Hit GAGTACAAGCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCA 7119 0.1568014702643269 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5955 0.13116347175503112 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5883 0.12957761617713653 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5653 0.12451168863663997 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5605 0.12345445158471025 No Hit GCTTACTGCTTGTACTCTGCGTTGATACCACTGCTTACTGCTTGTACTCT 5580 0.12290380728683017 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4849 0.10680296801681712 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4757 0.10477659700061848 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.202577191520254E-5 2 0.0 0.0 0.0 0.0 2.202577191520254E-5 3 0.0 0.0 0.0 0.0 2.202577191520254E-5 4 0.0 0.0 0.0 0.0 2.202577191520254E-5 5 0.0 0.0 0.0 2.202577191520254E-5 2.202577191520254E-5 6 0.0 0.0 0.0 2.202577191520254E-5 2.202577191520254E-5 7 0.0 0.0 0.0 2.202577191520254E-5 2.202577191520254E-5 8 0.0 0.0 0.0 2.202577191520254E-5 2.202577191520254E-5 9 0.0 0.0 0.0 6.607731574560762E-5 2.202577191520254E-5 10-11 0.0 0.0 0.0 7.709020170320889E-5 2.202577191520254E-5 12-13 0.0 0.0 0.0 9.911597361841144E-5 2.202577191520254E-5 14-15 0.0 0.0 0.0 1.3215463149121524E-4 2.202577191520254E-5 16-17 1.101288595760127E-5 0.0 0.0 1.8721906127922158E-4 2.202577191520254E-5 18-19 2.202577191520254E-5 0.0 0.0 2.4228349106722794E-4 2.202577191520254E-5 20-21 2.202577191520254E-5 0.0 0.0 4.6254121021925337E-4 2.202577191520254E-5 22-23 2.202577191520254E-5 0.0 0.0 9.250824204385067E-4 2.202577191520254E-5 24-25 2.202577191520254E-5 0.0 0.0 0.0016519328936401905 2.202577191520254E-5 26-27 2.202577191520254E-5 0.0 0.0 0.002885376120891533 2.202577191520254E-5 28-29 3.303865787280381E-5 0.0 0.0 0.00702622124094961 2.202577191520254E-5 30-31 4.405154383040508E-5 1.101288595760127E-5 0.0 0.01840253243515172 2.202577191520254E-5 32-33 4.405154383040508E-5 2.202577191520254E-5 0.0 0.04145250274441118 2.202577191520254E-5 34-35 4.405154383040508E-5 2.202577191520254E-5 0.0 0.06337915868599531 2.202577191520254E-5 36-37 4.405154383040508E-5 4.405154383040508E-5 0.0 0.08031697728878606 2.202577191520254E-5 38-39 4.405154383040508E-5 6.607731574560762E-5 0.0 0.09951243751288508 2.202577191520254E-5 40-41 4.405154383040508E-5 7.709020170320889E-5 0.0 0.13391669324443145 2.202577191520254E-5 42-43 4.405154383040508E-5 1.101288595760127E-4 0.0 0.17651453612843315 2.202577191520254E-5 44-45 4.405154383040508E-5 1.101288595760127E-4 0.0 0.2114914619297748 2.202577191520254E-5 46-47 4.405154383040508E-5 1.3215463149121524E-4 0.0 0.25013567875499765 2.202577191520254E-5 48-49 4.405154383040508E-5 1.5418040340641777E-4 0.0 0.28386814844313035 2.202577191520254E-5 50-51 4.405154383040508E-5 1.5418040340641777E-4 0.0 0.337985470038783 2.202577191520254E-5 52-53 4.405154383040508E-5 1.5418040340641777E-4 0.0 0.5199513847162287 2.202577191520254E-5 54-55 4.405154383040508E-5 1.5418040340641777E-4 0.0 0.7253967722552805 2.202577191520254E-5 56-57 4.405154383040508E-5 1.5418040340641777E-4 0.0 0.8722536064998934 2.202577191520254E-5 58-59 4.405154383040508E-5 1.5418040340641777E-4 0.0 1.1324881016780113 2.202577191520254E-5 60-61 5.506442978800635E-5 1.5418040340641777E-4 0.0 1.4348689114158695 2.202577191520254E-5 62-63 6.607731574560762E-5 1.5418040340641777E-4 0.0 1.5598211154908135 2.202577191520254E-5 64-65 6.607731574560762E-5 1.5418040340641777E-4 0.0 1.6658752072625138 2.202577191520254E-5 66-67 6.607731574560762E-5 1.9823194723682285E-4 0.0 2.1367972236955017 2.202577191520254E-5 68-69 6.607731574560762E-5 2.202577191520254E-4 0.0 2.488174363058728 2.202577191520254E-5 70-71 6.607731574560762E-5 2.3127060510962669E-4 0.0 2.583490891021767 2.202577191520254E-5 72-73 6.607731574560762E-5 2.4228349106722794E-4 0.0 2.7619106564208646 2.202577191520254E-5 74-75 6.607731574560762E-5 2.4228349106722794E-4 0.0 3.0417370757175557 2.202577191520254E-5 76-77 6.607731574560762E-5 2.643092629824305E-4 0.0 3.2728645133097336 2.202577191520254E-5 78-79 6.607731574560762E-5 2.643092629824305E-4 0.0 3.4710083574588957 2.202577191520254E-5 80-81 6.607731574560762E-5 2.643092629824305E-4 0.0 3.799698951749463 2.202577191520254E-5 82-83 6.607731574560762E-5 2.643092629824305E-4 0.0 4.1964271554860915 2.202577191520254E-5 84-85 6.607731574560762E-5 2.643092629824305E-4 0.0 4.613595275560027 2.202577191520254E-5 86-87 6.607731574560762E-5 2.643092629824305E-4 0.0 5.35982842804709 2.202577191520254E-5 88-89 6.607731574560762E-5 2.643092629824305E-4 0.0 6.315901109570286 2.202577191520254E-5 90-91 8.810308766081016E-5 2.643092629824305E-4 0.0 7.348601451586472 2.202577191520254E-5 92-93 8.810308766081016E-5 2.643092629824305E-4 0.0 8.353174882866945 2.202577191520254E-5 94-95 8.810308766081016E-5 2.643092629824305E-4 0.0 9.246286895370535 2.202577191520254E-5 96-97 8.810308766081016E-5 2.643092629824305E-4 0.0 10.19152289711145 2.202577191520254E-5 98-99 8.810308766081016E-5 2.643092629824305E-4 0.0 11.173597002380546 2.202577191520254E-5 100-101 8.810308766081016E-5 2.643092629824305E-4 0.0 12.035035954870075 2.202577191520254E-5 102-103 8.810308766081016E-5 2.643092629824305E-4 0.0 12.980800575136957 2.202577191520254E-5 104-105 8.810308766081016E-5 2.643092629824305E-4 0.0 13.95534186641105 2.202577191520254E-5 106-107 8.810308766081016E-5 2.643092629824305E-4 0.0 14.918572923806687 2.202577191520254E-5 108-109 8.810308766081016E-5 2.643092629824305E-4 0.0 15.862520417890565 4.405154383040508E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3505 0.0 31.343803 2 TATAACG 775 0.0 28.202543 2 ACCTGGG 3975 0.0 26.163958 3 TAACGCA 855 0.0 25.535862 4 GTACATG 19025 0.0 25.240847 1 GTACACG 790 0.0 24.810493 1 TACATGG 18830 0.0 23.703787 2 ACATGGG 19020 0.0 22.536701 3 GTATAAC 1295 0.0 22.257883 1 GAGTACT 12890 0.0 21.930212 12-13 AGTACTT 13610 0.0 21.318855 12-13 CATGGGT 3885 0.0 21.148428 4 TACACGG 725 0.0 20.627266 2 CATGGGG 7825 0.0 20.265522 4 GTACTTT 14185 0.0 20.15224 14-15 GTACTAG 1455 0.0 19.81028 1 GTACCTG 6310 0.0 19.185518 1 GAGTACG 1290 0.0 18.322205 1 GTATACG 1300 0.0 18.181265 1 GTATTAG 2720 0.0 17.803032 1 >>END_MODULE