FastQCFastQC Report
Wed 25 May 2016
SRR1003814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4540136
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT514601.1334462227563227No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT250740.5522742050017885No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC183620.4044372239069491No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT156530.3447694077886654No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT126730.27913260748136176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121260.26708451024374597No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC115010.25331840279674445No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT109790.2418209498570087No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC108940.2399487592442165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99050.21816527082008114No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG98760.2175265234345403No Hit
GTACAAGCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCAGT83800.18457596864939727No Hit
GAGTACAAGCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCA81320.17911357721442706No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA81240.17893737103910543No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC80970.17834267519739497No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA74800.164752773925715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70060.154312558037909No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA62620.1379253837329983No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA56150.12367470930386226No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC53390.11759559625526636No Hit
GCTTACTGCTTGTACTCTGCGTTGATACCACTGCTTACTGCTTGTACTCT49970.1100627822602671No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT48010.1057457309648874No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA46800.10308061256314789No Hit
GCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCAGTGGTATC46730.10292643215974147No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC45800.10087803537162762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG37450.033.3700182
ACCTGGG39050.030.4198533
GTACACG6250.028.9036271
TAACGCA6150.027.7997384
TATAACG6750.024.6386412
GTACCTG54500.023.4641341
CTAACGC3650.022.1306533
GTATAAC10750.021.2266541
GGCGTCG6900.019.9602978
AGTACTT162050.019.40198512-13
GTACATG195100.019.1519951
TACACGG6750.019.0069522
CATGGGT38950.018.7770164
GAGTACT153750.018.75041212-13
GAGTACG10900.018.3177261
TACATGG193100.017.8651582
ACGTGCG3251.0057192E-817.5363779
ATAACGC10400.017.3615473
ACATGGG190200.017.3125423
GTATACG11550.017.286861
Produced by FastQC (version 0.11.5)