##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003814_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4540136 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.526193708734716 31.0 31.0 34.0 30.0 34.0 2 31.757338766944425 31.0 31.0 34.0 30.0 34.0 3 31.787495572819847 31.0 31.0 34.0 30.0 34.0 4 35.562895692992456 37.0 35.0 37.0 33.0 37.0 5 35.41235725097222 37.0 35.0 37.0 33.0 37.0 6 35.443790670587845 37.0 35.0 37.0 33.0 37.0 7 35.36077333366225 37.0 35.0 37.0 33.0 37.0 8 35.35363081634559 37.0 35.0 37.0 32.0 37.0 9 36.90219566109914 39.0 37.0 39.0 33.0 39.0 10-11 36.81804664001255 39.0 37.0 39.0 32.0 39.0 12-13 36.75874511248121 39.0 35.5 39.0 32.0 39.0 14-15 37.82649638689237 40.0 37.0 41.0 32.5 41.0 16-17 37.84043991193215 40.0 37.0 41.0 32.5 41.0 18-19 37.84379708008747 40.0 37.0 41.0 32.0 41.0 20-21 37.77765610986103 39.5 37.0 41.0 32.0 41.0 22-23 37.692394677163854 39.0 37.0 41.0 32.0 41.0 24-25 37.60048168160601 39.0 36.5 41.0 32.0 41.0 26-27 37.38114959551872 39.0 36.0 41.0 31.5 41.0 28-29 37.194948785675145 39.0 36.0 40.5 31.0 41.0 30-31 36.98466962223158 39.0 36.0 40.0 31.0 41.0 32-33 36.74625683019187 38.5 35.0 40.0 30.0 41.0 34-35 36.72206999966521 39.0 35.0 40.0 30.0 41.0 36-37 36.54722512717681 38.5 35.0 40.0 30.0 41.0 38-39 36.316080839869116 38.0 35.0 40.0 30.0 41.0 40-41 36.45765203949838 39.0 35.0 40.0 30.0 41.0 42-43 36.31689722510515 39.0 35.0 40.0 30.0 41.0 44-45 36.158334574118484 39.0 35.0 40.0 30.0 41.0 46-47 35.890260666200305 38.5 35.0 40.0 28.5 41.0 48-49 35.66504340398613 38.0 35.0 40.0 28.0 41.0 50-51 35.42494486949289 38.0 34.0 40.0 27.0 41.0 52-53 35.238547479634974 38.0 34.0 40.0 27.0 41.0 54-55 35.021542085963944 38.0 34.0 40.0 26.0 41.0 56-57 34.77580803746848 38.0 34.0 40.0 26.0 41.0 58-59 34.512528038807645 38.0 33.0 40.0 26.0 41.0 60-61 34.080783042622514 37.0 33.0 40.0 24.0 41.0 62-63 33.8639285254891 37.0 33.0 40.0 24.0 41.0 64-65 33.41511223452337 36.0 32.0 39.0 22.5 41.0 66-67 33.04618859435048 36.0 32.0 39.0 22.0 40.5 68-69 32.770238600782 35.5 31.0 39.0 22.0 40.0 70-71 32.36258242043851 35.0 31.0 38.0 21.5 40.0 72-73 31.98930329399824 35.0 31.0 37.5 20.5 40.0 74-75 31.603501304806727 35.0 31.0 37.0 20.0 39.0 76-77 30.61053446416583 34.0 29.5 36.0 19.5 39.0 78-79 30.998515793359495 34.0 30.5 36.0 19.5 39.0 80-81 30.82014657710694 34.0 30.5 36.0 18.0 37.5 82-83 30.498697175591218 34.0 30.0 35.0 18.0 37.0 84-85 30.145690679750565 34.0 30.0 35.0 14.5 37.0 86-87 29.81173328287963 34.0 30.0 35.0 10.5 36.0 88-89 29.497115174523408 34.0 29.0 35.0 7.0 36.0 90-91 29.19922751212739 34.0 29.0 35.0 4.0 35.5 92-93 28.936490999388568 34.0 29.0 35.0 2.0 35.0 94-95 28.59158569258718 34.0 29.0 35.0 2.0 35.0 96-97 28.16592817924397 34.0 29.0 35.0 2.0 35.0 98-99 27.744637165054087 34.0 29.0 35.0 2.0 35.0 100-101 26.715737259853007 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04349839413773893 1101 2 0.07083997665368358 1101 3 0.0885413717331005 1101 4 0.14203701192325724 1101 5 0.13158827084620128 1101 6 0.1761088335522345 1101 7 0.04010767188886177 1101 8 0.15164684166644804 1101 9 0.22509711342581795 1101 10-11 0.1789255835342516 1101 12-13 0.16278813956097338 1101 14-15 0.13552383594466022 1101 16-17 0.1486213282868718 1101 18-19 0.025089631386222777 1101 20-21 0.05976256425082482 1101 22-23 0.10295117863242353 1101 24-25 0.09788261391534547 1101 26-27 0.05373022610422851 1101 28-29 0.02109405393833441 1101 30-31 0.07418510770073539 1101 32-33 0.13807092897713602 1101 34-35 0.251206636050199 1101 36-37 0.1433916197168159 1101 38-39 0.14418251552262262 1101 40-41 0.15590721000101837 1101 42-43 0.03641561896918688 1101 44-45 -0.0069692407562413905 1101 46-47 0.07196635108363125 1101 48-49 0.20483754372366292 1101 50-51 0.10772352343368397 1101 52-53 0.17543283107197993 1101 54-55 0.17916926649626674 1101 56-57 0.1757139900267788 1101 58-59 0.016655023123384183 1101 60-61 0.02854695718176714 1101 62-63 -0.012103360679937225 1101 64-65 -0.19126248171078686 1101 66-67 -0.15234826582668148 1101 68-69 -0.22332656738186785 1101 70-71 -0.11854615148907044 1101 72-73 -0.1822901506909993 1101 74-75 -0.1457153297531235 1101 76-77 -0.15049671483407323 1101 78-79 -0.10706562566820921 1101 80-81 -0.10668101132814911 1101 82-83 -0.17136161023323382 1101 84-85 -0.22073982344214116 1101 86-87 -0.2177943580940962 1101 88-89 -0.09389934051903737 1101 90-91 -0.17568858729387316 1101 92-93 -0.1167120164333113 1101 94-95 -0.034331646206499755 1101 96-97 0.0335196566940148 1101 98-99 -0.11121738326100328 1101 100-101 -0.0768913555440669 1102 1 1.3713144981863934E-4 1102 2 -0.0025708715530896598 1102 3 0.041515884183411345 1102 4 0.018746759788626832 1102 5 -0.06801217805927706 1102 6 -0.017514037747908162 1102 7 -0.061241916174751054 1102 8 -0.06661703715428757 1102 9 -0.013788682058837765 1102 10-11 -0.06919499245994842 1102 12-13 0.01029827600461175 1102 14-15 0.08092746916544513 1102 16-17 -0.003601778765698782 1102 18-19 -0.11781009539031118 1102 20-21 -0.12578848310581492 1102 22-23 -0.2886822485302787 1102 24-25 -0.23890137098229758 1102 26-27 -0.21471450596843766 1102 28-29 -0.057902058177838 1102 30-31 -0.1777171491302667 1102 32-33 -0.15814608278503073 1102 34-35 -0.25214619390104787 1102 36-37 -0.3062898997259893 1102 38-39 -0.2787468238873174 1102 40-41 -0.08624834877634413 1102 42-43 -0.08494778085248811 1102 44-45 0.04975204959254853 1102 46-47 -0.17204475995744417 1102 48-49 -0.11609607135459754 1102 50-51 -0.06042634301297056 1102 52-53 -0.032560969045540844 1102 54-55 -0.15597229585264216 1102 56-57 -0.05144712637230242 1102 58-59 -0.1803719702721409 1102 60-61 -0.25467675290953196 1102 62-63 -0.3833203899519404 1102 64-65 -0.3884493282291501 1102 66-67 -0.2692147430602745 1102 68-69 -0.3563604358391146 1102 70-71 -0.33407179557669053 1102 72-73 -0.39519608995708566 1102 74-75 -0.2619251414710284 1102 76-77 -0.2555851896970722 1102 78-79 -0.21373813361592653 1102 80-81 -0.2954515804234781 1102 82-83 -0.39140107973658544 1102 84-85 -0.4815591156385679 1102 86-87 -0.4645343185965771 1102 88-89 -0.43863337429247906 1102 90-91 -0.3755796527720179 1102 92-93 -0.27657601871005966 1102 94-95 -0.39334644481888503 1102 96-97 -0.35739626411203673 1102 98-99 -0.49730866740197044 1102 100-101 -0.5185900010065545 1103 1 -0.042821866142226384 1103 2 -0.14673845257749818 1103 3 -0.0694725289409277 1103 4 -0.05564437870550165 1103 5 0.026916626776397834 1103 6 0.027071325203742447 1103 7 0.030421204207179642 1103 8 0.04737299614640733 1103 9 -0.0649033509158059 1103 10-11 0.03851053652979175 1103 12-13 -0.02574011694548517 1103 14-15 0.027144522900890422 1103 16-17 -0.09972981155879523 1103 18-19 -0.049510594492907956 1103 20-21 0.015531387299269284 1103 22-23 -0.005935519468195594 1103 24-25 -0.031434550534022776 1103 26-27 -0.04037854078807612 1103 28-29 -0.15125180277336625 1103 30-31 -0.021674735724779737 1103 32-33 0.020688474516290967 1103 34-35 -0.10698863570879524 1103 36-37 -0.17948543251068827 1103 38-39 -0.011649285624621086 1103 40-41 -0.05145987126714857 1103 42-43 0.1026074704235711 1103 44-45 0.15108151055886765 1103 46-47 0.221526997255026 1103 48-49 0.21505588506712314 1103 50-51 0.02510452741562119 1103 52-53 0.08817762411171515 1103 54-55 -0.04279690852398943 1103 56-57 0.08653588600427042 1103 58-59 0.03255554494730717 1103 60-61 0.17144260567910408 1103 62-63 0.07191054394994723 1103 64-65 0.02356550043852934 1103 66-67 -0.19472669144108323 1103 68-69 -0.09852872787962497 1103 70-71 -0.09263181526636544 1103 72-73 0.056610860057329404 1103 74-75 0.07896660800757971 1103 76-77 0.10908791321650924 1103 78-79 0.11339094459152577 1103 80-81 0.06980014662118705 1103 82-83 0.02387744234785316 1103 84-85 -0.09008715879465612 1103 86-87 -0.10826499894110952 1103 88-89 -0.16717150696846517 1103 90-91 -0.16813931551434536 1103 92-93 -0.2085588132719849 1103 94-95 -0.38444612572522274 1103 96-97 -0.28196645200355874 1103 98-99 -0.2690025744929905 1103 100-101 -0.2560113490489364 1104 1 -0.018638574283819764 1104 2 -0.013723114181701135 1104 3 -0.02141772814113807 1104 4 -1.7467573179885676E-4 1104 5 0.0386124336814575 1104 6 -0.04810684017764544 1104 7 0.01749065185455123 1104 8 -0.01971876371711545 1104 9 -0.062497398495345635 1104 10-11 0.032449963562136475 1104 12-13 0.01825275993765274 1104 14-15 -0.022185964060057017 1104 16-17 0.004150961519123086 1104 18-19 -0.08128713591786152 1104 20-21 -0.08296487825524679 1104 22-23 -0.07936557310998182 1104 24-25 0.046710871069763016 1104 26-27 0.12275110351276197 1104 28-29 0.043466344051374506 1104 30-31 0.009134569522650793 1104 32-33 0.05484432853248222 1104 34-35 0.09315479388816783 1104 36-37 0.17418141824735756 1104 38-39 0.06524779878198217 1104 40-41 0.13993827206676457 1104 42-43 0.08074030724910841 1104 44-45 0.2869451832774743 1104 46-47 0.18081526327776487 1104 48-49 0.2110634563571523 1104 50-51 0.250746008357531 1104 52-53 0.23162381482415384 1104 54-55 0.052756116838743594 1104 56-57 0.05153902179818459 1104 58-59 0.1235986859642324 1104 60-61 0.16227550242180655 1104 62-63 0.12259458006892032 1104 64-65 0.1633103858986047 1104 66-67 0.20716475061660589 1104 68-69 0.07151625679313867 1104 70-71 0.03500986536788986 1104 72-73 0.21471271525787827 1104 74-75 0.12233929926571818 1104 76-77 -0.022284738140978533 1104 78-79 0.23107410393628314 1104 80-81 0.12395230084494813 1104 82-83 0.2520315059406002 1104 84-85 0.16284761905564338 1104 86-87 0.14284706619263687 1104 88-89 0.20697456477006781 1104 90-91 0.27320157700645 1104 92-93 0.1051489082316941 1104 94-95 0.15844347929522584 1104 96-97 0.23734841697339348 1104 98-99 0.24197282240737295 1104 100-101 0.2519527779981381 1105 1 0.0666289244234406 1105 2 0.07122243547992824 1105 3 0.13116112607609764 1105 4 0.09794720344524421 1105 5 -0.0353550771412543 1105 6 0.11271842819088818 1105 7 0.0616694036129104 1105 8 0.16443171300752368 1105 9 0.11813973866595973 1105 10-11 0.15818215053094065 1105 12-13 0.08135985628768339 1105 14-15 0.14850640713137864 1105 16-17 0.10269412796706234 1105 18-19 0.0862303658253154 1105 20-21 0.0378477719699859 1105 22-23 0.06534734577326162 1105 24-25 0.07497510255903705 1105 26-27 0.1808069887836794 1105 28-29 0.2515086876935797 1105 30-31 0.16909657671116207 1105 32-33 0.15689236220044478 1105 34-35 0.20535933150578956 1105 36-37 0.1585315175008617 1105 38-39 0.20364691562492254 1105 40-41 0.21098381288891233 1105 42-43 0.310524872503521 1105 44-45 0.3670427874964801 1105 46-47 0.2965827036670632 1105 48-49 0.1915480489772321 1105 50-51 0.3255263699176041 1105 52-53 0.3126785114253039 1105 54-55 0.36780945165190815 1105 56-57 0.3773304869289902 1105 58-59 0.2968167332980869 1105 60-61 0.31736146422905165 1105 62-63 0.19158451859642156 1105 64-65 0.2335975107891315 1105 66-67 0.2098576143335933 1105 68-69 0.2646143046805136 1105 70-71 0.4145194465997335 1105 72-73 0.30715727718547825 1105 74-75 0.10260692929429993 1105 76-77 0.13085682087750783 1105 78-79 0.19136281880323835 1105 80-81 0.30150952940589804 1105 82-83 0.2301818973769798 1105 84-85 0.18155003449247786 1105 86-87 0.4017398495353319 1105 88-89 0.2952202333551526 1105 90-91 0.29248222987392936 1105 92-93 0.2626375559976921 1105 94-95 0.3595358185176849 1105 96-97 0.4029178445790542 1105 98-99 0.3811643857608722 1105 100-101 0.3683528906238216 1106 1 -0.06806412856956712 1106 2 -0.06444239636796922 1106 3 -0.04019598700618232 1106 4 -0.06411207998647939 1106 5 -0.06471867760187422 1106 6 -0.09173237468044704 1106 7 -0.06764794333145119 1106 8 -0.04616307229620986 1106 9 -0.11042506236174177 1106 10-11 -0.2085223147580777 1106 12-13 -0.17351243302736208 1106 14-15 -0.09409609464433544 1106 16-17 -0.0940452870025652 1106 18-19 -0.15742196355134297 1106 20-21 -0.11858647658123544 1106 22-23 -0.11211356461963362 1106 24-25 -0.22073211890467803 1106 26-27 -0.11486905496856537 1106 28-29 -0.1423398639380622 1106 30-31 -0.13607933661935334 1106 32-33 -0.009684764307515081 1106 34-35 -0.11632804709730493 1106 36-37 -0.08240145613036276 1106 38-39 -0.027477568549947762 1106 40-41 -0.16485976126634938 1106 42-43 -0.08779430294514157 1106 44-45 -0.06474908542363522 1106 46-47 -0.19114583731540336 1106 48-49 -0.22589269781694554 1106 50-51 -0.0656240212408008 1106 52-53 -0.13334400190032625 1106 54-55 -0.10685035842545432 1106 56-57 -0.06997934319218047 1106 58-59 -0.1301544804114485 1106 60-61 0.005615829039015807 1106 62-63 -0.1600137302887248 1106 64-65 -0.0944856630805404 1106 66-67 -0.19159692921309812 1106 68-69 -0.11038482475792222 1106 70-71 -0.028585756888340086 1106 72-73 -0.04870358934039487 1106 74-75 -0.09537585652313041 1106 76-77 -0.13562420956280263 1106 78-79 -0.1209015317889417 1106 80-81 -0.05983264809004396 1106 82-83 -0.01827720865518856 1106 84-85 0.05158146106847994 1106 86-87 0.031952783786525885 1106 88-89 0.0842806592422285 1106 90-91 0.017718792791015403 1106 92-93 -0.0555110246712367 1106 94-95 0.03171548459534179 1106 96-97 0.20080936304364272 1106 98-99 0.13866925324468937 1106 100-101 0.170969329741947 1107 1 -0.13583891649305357 1107 2 -0.10838533123722982 1107 3 -0.14285012938305286 1107 4 -0.11060366893021722 1107 5 -0.16673897525370762 1107 6 -0.11887001523079732 1107 7 -0.2429556795872827 1107 8 -0.11149406208289037 1107 9 -0.1617593990171997 1107 10-11 -0.07541873462744064 1107 12-13 -0.11724271395561914 1107 14-15 -0.08901219621538559 1107 16-17 -0.1592394886755173 1107 18-19 -0.21010321464144965 1107 20-21 -0.16485483180822058 1107 22-23 -0.19175169413142612 1107 24-25 -0.11885434998249167 1107 26-27 -0.18347250976989216 1107 28-29 -0.27785645366776635 1107 30-31 -0.16423145163941655 1107 32-33 -0.18315478589178014 1107 34-35 -0.2018614079467227 1107 36-37 -0.2367865085711145 1107 38-39 -0.07456250396734276 1107 40-41 -0.17299538008838766 1107 42-43 -0.08835926027913388 1107 44-45 -0.1527122965071115 1107 46-47 -0.3617659121864918 1107 48-49 -0.44400331849906394 1107 50-51 -0.2916616028107981 1107 52-53 -0.297824488044391 1107 54-55 -0.4331027690917111 1107 56-57 -0.4242316752837638 1107 58-59 -0.35562827071007064 1107 60-61 -0.31244191967351753 1107 62-63 -0.41127564548646234 1107 64-65 -0.4111381718036924 1107 66-67 -0.33822593863529704 1107 68-69 -0.3052390442832902 1107 70-71 -0.25125639994810456 1107 72-73 -0.32206923038174295 1107 74-75 -0.23500685331964277 1107 76-77 -0.3488364927407481 1107 78-79 -0.3141313364030438 1107 80-81 -0.1666046284487095 1107 82-83 -0.2572946367614861 1107 84-85 -0.25527679446866713 1107 86-87 -0.1332283265912082 1107 88-89 -0.30790765861334535 1107 90-91 -0.22519628928252544 1107 92-93 -0.3549163910438473 1107 94-95 -0.12581589627977152 1107 96-97 -0.06325598687223888 1107 98-99 -0.054369788011459974 1107 100-101 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-0.8500426754337731 2316 78-79 -0.6209605346992326 2316 80-81 -0.7330977374103895 2316 82-83 -0.7764090770191672 2316 84-85 -0.8324868804767185 2316 86-87 -0.7370972632434878 2316 88-89 -0.8406568554807059 2316 90-91 -0.9946327424908361 2316 92-93 -0.9200858107349674 2316 94-95 -1.089346230948042 2316 96-97 -0.7360960151869698 2316 98-99 -0.9988509392020717 2316 100-101 -1.5204068738145935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 59.0 5 101.0 6 255.0 7 734.0 8 1636.0 9 3071.0 10 5217.0 11 8225.0 12 11797.0 13 16236.0 14 19950.0 15 26146.0 16 32127.0 17 40182.0 18 35253.0 19 26182.0 20 24276.0 21 25172.0 22 27522.0 23 31103.0 24 36992.0 25 45667.0 26 57315.0 27 73002.0 28 91038.0 29 113737.0 30 140954.0 31 171745.0 32 210326.0 33 260338.0 34 333131.0 35 432145.0 36 595316.0 37 796817.0 38 712983.0 39 133307.0 40 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98548496104988 20.57698321038223 14.853140701496677 21.58439112707121 2 15.025025887139781 21.722179281962 39.525791212535786 23.727003618362435 3 20.729174929709053 26.062869633429404 29.877420520549634 23.33053491631191 4 12.881882833465783 16.83286579961481 38.97801739859775 31.30723396832165 5 12.377140244257 39.155655249093854 34.91540782038247 13.551796686266668 6 29.21427311226718 38.599265344798305 17.170346220085115 15.016115322849396 7 26.992501324299827 33.13207646909178 22.21839218437337 17.65703002223502 8 24.89821890797985 34.75688833990876 19.601945844794077 20.742946907317314 9 24.256123605107867 18.883905680358474 22.405077733354243 34.45489298117942 10-11 24.642402033690963 26.63815346710588 27.851821961954965 20.86762253724819 12-13 26.5365508874624 24.21844852224691 27.979943772609456 21.265056817681234 14-15 22.370009987816164 26.33951989099635 27.453267670059116 23.837202451128366 16-17 20.72730860925752 29.58014914090679 28.104510525675884 21.588031724159805 18-19 20.51018956260341 29.812289764007073 29.759505001612286 19.918015671777233 20-21 21.873608425943395 27.408161757036442 29.052734509450563 21.6654953075696 22-23 20.92280936077686 27.55631108847841 29.503554519071674 22.01732503167306 24-25 21.85869542233977 27.820488196829345 29.44012029595589 20.88069608487499 26-27 21.566045065146515 26.97827032457668 30.62220396887047 20.833480641406332 28-29 20.595983323912687 28.77042492478535 29.866715098925606 20.76687665237636 30-31 21.34893201205906 27.71666940549266 29.318772471406433 21.615626111041845 32-33 21.66462364987428 27.903578418253446 29.579391852823495 20.85240607904878 34-35 22.170353489117957 27.125602343025157 29.993708935162385 20.710335232694504 36-37 21.745497147756648 27.016639811915404 30.690794966727868 20.547068073600084 38-39 21.409329874038857 28.235742195330303 30.076565306518955 20.27836262411188 40-41 21.137903160697704 28.184039766924112 29.542984587794628 21.135072484583553 42-43 20.8019649631641 28.39363182072079 28.86325431661078 21.94114889950433 44-45 21.66421886921449 27.645658632252424 28.73484406634515 21.955278432187935 46-47 22.212123394677377 28.29680465167195 28.662234635960942 20.828837317689732 48-49 22.18705563005161 28.098893953837507 28.755328034226284 20.958722381884595 50-51 20.950705000907462 28.652776040189103 29.464723083185174 20.931795875718258 52-53 21.369965569313344 29.088633027733092 27.937885561137378 21.603515841816193 54-55 20.953226951791752 28.43741905528821 28.460546115799172 22.148807877120863 56-57 22.018647693134856 27.917300557985886 28.30838406115841 21.75566768772085 58-59 22.74408394397201 27.465464320519033 28.185984368046373 21.604467367462583 60-61 21.60592850857817 28.09707443776741 29.494977797500116 20.8020192561543 62-63 21.2329556243741 28.3763603448329 28.945644363201552 21.44503966759144 64-65 21.043017879752483 29.491191012483785 28.253675571360137 21.21211553640359 66-67 21.244522012850194 27.92960505618324 28.161282164035146 22.66459076693142 68-69 20.883977401617752 28.648291944191477 28.56515426725134 21.902576386939423 70-71 21.785869292647472 28.426124026030593 27.961863118360654 21.826143562961285 72-73 21.52360634130784 27.42775767069533 28.926567397981028 22.122068590015807 74-75 20.991970284590593 29.215512486850614 28.378819489107816 21.413697739450978 76-77 21.512296107429382 28.040525658262222 28.016176167409963 22.431002066898436 78-79 21.434963622235102 28.23766732978924 28.114356045721976 22.213013002253675 80-81 22.005026061446845 28.185403277060782 27.79834434456857 22.0112263169238 82-83 21.595641628356507 27.88544219820728 29.08625424436625 21.432661929069965 84-85 21.02845654208186 28.228597242790325 28.46171814110752 22.28122807402029 86-87 21.097858352552795 28.398276426768295 27.723258701399505 22.78060651927941 88-89 21.145633082357005 28.632578407342862 27.556377165794153 22.66541134450598 90-91 21.531238271276454 27.038308984576677 28.097385188461317 23.333067555685556 92-93 21.184068054349034 27.89667534188403 28.169200217790834 22.7500563859761 94-95 20.856749948707467 27.974732907141846 28.591619917868073 22.576897226282615 96-97 20.43601423484409 28.0531910230705 29.414705800080043 22.09608894200537 98-99 20.368906873745075 28.661877802241175 28.131494878104434 22.837720445909312 100-101 19.52916965991059 28.119800827539482 28.557920329914495 23.793109182635437 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 320.0 1 224.0 2 299.5 3 455.5 4 394.5 5 366.5 6 550.0 7 1087.5 8 1683.0 9 2133.5 10 2347.5 11 2424.5 12 2730.0 13 3148.0 14 3612.0 15 4171.5 16 4709.5 17 5267.0 18 5926.0 19 6578.5 20 7318.0 21 8373.0 22 9836.5 23 11735.0 24 14279.0 25 17616.5 26 21878.5 27 27443.0 28 35219.0 29 44554.5 30 53791.5 31 63411.0 32 74535.5 33 85479.0 34 95708.5 35 107731.0 36 119375.5 37 132975.5 38 145571.5 39 152136.0 40 157766.5 41 164238.5 42 170293.5 43 177863.0 44 181864.0 45 185421.0 46 198974.5 47 250378.0 48 332354.0 49 307170.0 50 215719.5 51 170874.5 52 138984.0 53 116701.0 54 100821.0 55 85928.5 56 70747.5 57 56190.5 58 44617.5 59 36727.5 60 29601.5 61 22376.0 62 16398.5 63 11436.5 64 7537.5 65 4784.5 66 2851.5 67 1698.5 68 1003.0 69 560.0 70 331.0 71 218.0 72 140.5 73 87.5 74 63.0 75 54.0 76 40.0 77 24.5 78 14.0 79 11.5 80 11.5 81 7.0 82 5.5 83 4.0 84 2.0 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04742148693343107 2 0.005220107943903002 3 0.04087983267461592 4 0.0 5 0.0 6 0.0010352112800145195 7 2.202577191520254E-5 8 0.0 9 0.0 10-11 3.634252366008419E-4 12-13 0.0 14-15 0.0023016931651386654 16-17 0.0 18-19 0.0 20-21 0.011838852404421365 22-23 0.0 24-25 0.0 26-27 0.00727951761797444 28-29 0.010175906624823573 30-31 0.010528318975466815 32-33 0.006189241908171914 34-35 0.008017380977133724 36-37 0.01986724626751269 38-39 0.010616422063127623 40-41 0.012874063684435885 42-43 0.0 44-45 0.0 46-47 1.1012885957601269E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0012554689991665447 58-59 2.7532214894003174E-4 60-61 0.00979045561630753 62-63 0.01458106100786408 64-65 0.0425097397963409 66-67 0.023347318230114694 68-69 0.0016849715515129945 70-71 3.634252366008419E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.101288595760127E-5 82-83 0.0 84-85 5.5064429788006345E-5 86-87 7.268504732016838E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 3.303865787280381E-5 96-97 1.9823194723682285E-4 98-99 4.4051543830405076E-4 100-101 0.0011123014817177282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4540136.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.789887782897935 #Duplication Level Percentage of deduplicated Percentage of total 1 72.76453339094274 33.318798185461986 2 15.668885421533224 14.349530102701838 3 4.9540928995762945 6.805420738129499 4 2.1109043295719685 3.866322894861354 5 1.087856091311556 2.490640417254906 6 0.7039358696102535 1.933988668548611 7 0.46845699615229547 1.5015415299448944 8 0.3429976615764273 1.2564659546704808 9 0.24387964406748616 1.0050499380944953 >10 1.4337074556739553 12.288757973565232 >50 0.13248064041622487 4.181310000700305 >100 0.07896869702403324 6.609840096283873 >500 0.005011885462220067 1.6174669668010282 >1k 0.003325193018135616 3.1933601124951085 >5k 5.301032347752343E-4 1.8583690260367745 >10k+ 4.337208284524644E-4 3.723137394449709 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 51460 1.1334462227563227 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 25074 0.5522742050017885 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18362 0.4044372239069491 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15653 0.3447694077886654 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12673 0.27913260748136176 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12126 0.26708451024374597 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11501 0.25331840279674445 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10979 0.2418209498570087 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10894 0.2399487592442165 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9905 0.21816527082008114 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9876 0.2175265234345403 No Hit GTACAAGCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCAGT 8380 0.18457596864939727 No Hit GAGTACAAGCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCA 8132 0.17911357721442706 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8124 0.17893737103910543 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8097 0.17834267519739497 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7480 0.164752773925715 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7006 0.154312558037909 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6262 0.1379253837329983 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5615 0.12367470930386226 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5339 0.11759559625526636 No Hit GCTTACTGCTTGTACTCTGCGTTGATACCACTGCTTACTGCTTGTACTCT 4997 0.1100627822602671 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4801 0.1057457309648874 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4680 0.10308061256314789 No Hit GCAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTAAGCAGTGGTATC 4673 0.10292643215974147 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4580 0.10087803537162762 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.202577191520254E-5 0.0 6 0.0 0.0 0.0 2.202577191520254E-5 0.0 7 0.0 0.0 0.0 2.202577191520254E-5 0.0 8 4.405154383040508E-5 0.0 0.0 2.202577191520254E-5 0.0 9 4.405154383040508E-5 0.0 0.0 4.405154383040508E-5 0.0 10-11 4.405154383040508E-5 0.0 0.0 5.506442978800635E-5 0.0 12-13 4.405154383040508E-5 0.0 0.0 9.911597361841144E-5 0.0 14-15 4.405154383040508E-5 0.0 0.0 1.7620617532162033E-4 0.0 16-17 4.405154383040508E-5 0.0 0.0 2.3127060510962669E-4 0.0 18-19 4.405154383040508E-5 0.0 0.0 2.973479208552343E-4 0.0 20-21 4.405154383040508E-5 0.0 0.0 5.28618525964861E-4 0.0 22-23 4.405154383040508E-5 0.0 0.0 0.0010462241659721207 0.0 24-25 4.405154383040508E-5 0.0 0.0 0.001839151954919412 0.0 26-27 4.405154383040508E-5 0.0 0.0 0.003138672497916362 0.0 28-29 4.405154383040508E-5 0.0 0.0 0.007510788223084066 0.0 30-31 4.405154383040508E-5 0.0 0.0 0.01960293700453026 0.0 32-33 5.506442978800635E-5 0.0 0.0 0.043655079935931436 0.0 34-35 6.607731574560762E-5 0.0 0.0 0.06624250903497164 0.0 36-37 6.607731574560762E-5 3.303865787280381E-5 0.0 0.08389616522500648 0.0 38-39 8.810308766081016E-5 7.709020170320889E-5 0.0 0.10378543726443437 0.0 40-41 8.810308766081016E-5 8.810308766081016E-5 0.0 0.1395883295125961 0.0 42-43 8.810308766081016E-5 8.810308766081016E-5 0.0 0.18360683468512837 0.0 44-45 8.810308766081016E-5 8.810308766081016E-5 0.0 0.2204669639852198 0.0 46-47 8.810308766081016E-5 8.810308766081016E-5 0.0 0.26044374001131243 0.0 48-49 8.810308766081016E-5 8.810308766081016E-5 0.0 0.29536560138286605 0.0 50-51 8.810308766081016E-5 8.810308766081016E-5 0.0 0.350385979627042 0.0 52-53 8.810308766081016E-5 8.810308766081016E-5 0.0 0.5351822059955913 0.0 54-55 9.911597361841144E-5 8.810308766081016E-5 0.0 0.7440195624095842 0.0 56-57 1.101288595760127E-4 1.101288595760127E-4 0.0 0.8928477032406078 0.0 58-59 1.101288595760127E-4 1.101288595760127E-4 0.0 1.1569587342758014 0.0 60-61 1.101288595760127E-4 1.101288595760127E-4 0.0 1.4656499276673651 0.0 62-63 1.101288595760127E-4 1.101288595760127E-4 0.0 1.593146108398515 0.0 64-65 1.101288595760127E-4 1.101288595760127E-4 0.0 1.701050365011092 0.0 66-67 1.101288595760127E-4 1.101288595760127E-4 0.0 2.18202714632337 0.0 68-69 1.3215463149121524E-4 1.101288595760127E-4 0.0 2.541619898610967 0.0 70-71 1.3215463149121524E-4 1.101288595760127E-4 0.0 2.639469390344254 0.0 72-73 1.3215463149121524E-4 1.101288595760127E-4 0.0 2.823803075502584 0.0 74-75 1.3215463149121524E-4 1.101288595760127E-4 0.0 3.111239398995977 0.0 76-77 1.3215463149121524E-4 1.3215463149121524E-4 0.0 3.3504502948810346 0.0 78-79 1.3215463149121524E-4 1.3215463149121524E-4 0.0 3.5565564555775424 0.0 80-81 1.3215463149121524E-4 1.3215463149121524E-4 0.0 3.896315000255499 0.0 82-83 1.3215463149121524E-4 1.5418040340641777E-4 0.0 4.304882056396549 0.0 84-85 1.3215463149121524E-4 1.5418040340641777E-4 0.0 4.733911054646821 0.0 86-87 1.3215463149121524E-4 1.5418040340641777E-4 0.0 5.502940881066118 0.0 88-89 1.3215463149121524E-4 1.5418040340641777E-4 0.0 6.488748354674838 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3745 0.0 33.370018 2 ACCTGGG 3905 0.0 30.419853 3 GTACACG 625 0.0 28.903627 1 TAACGCA 615 0.0 27.799738 4 TATAACG 675 0.0 24.638641 2 GTACCTG 5450 0.0 23.464134 1 CTAACGC 365 0.0 22.130653 3 GTATAAC 1075 0.0 21.226654 1 GGCGTCG 690 0.0 19.960297 8 AGTACTT 16205 0.0 19.401985 12-13 GTACATG 19510 0.0 19.151995 1 TACACGG 675 0.0 19.006952 2 CATGGGT 3895 0.0 18.777016 4 GAGTACT 15375 0.0 18.750412 12-13 GAGTACG 1090 0.0 18.317726 1 TACATGG 19310 0.0 17.865158 2 ACGTGCG 325 1.0057192E-8 17.536377 9 ATAACGC 1040 0.0 17.361547 3 ACATGGG 19020 0.0 17.312542 3 GTATACG 1155 0.0 17.28686 1 >>END_MODULE