FastQCFastQC Report
Wed 25 May 2016
SRR1003813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249485
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT203051.625069528645802No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT191001.528629795475736No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC112920.903732337723142No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT83690.6697959559338448No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT53470.42793630975962094No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT52930.4236145291860246No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC38390.3072465855932644No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38190.3056459261215621No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33470.2678703625893868No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG32540.2604272960459709No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA30480.24394050348743682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27910.22337202927606173No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27700.2216913368307743No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24810.19856180746467542No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24390.19520042257410053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23160.18535636682313114No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC22400.17927386083066224No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22230.17791330027971525No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA21280.1703101677891291No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT20870.16702881587213933No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20390.1631872331400537No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG18460.1477408692381261No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG17430.13949747295885906No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16960.13573592320035854No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC16960.13573592320035854No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC16520.1322144723626134No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC16020.12821282368335754No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG13530.1082846132606634No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT13410.107324217577642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.10492322837008848No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG12790.10236217321536473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10400.036.4958152
ACCTGGG11050.032.7519043
TATAACG1552.656052E-729.6818352
TAGCGTG1057.2904426E-427.330849
GGGTACG850.008389886527.1441271
GAGTACG4500.025.6361221
TATACCG2301.2032615E-824.9764335
AATACCG1150.001233369724.9764335
TACGCAG4200.023.2518734
TACCGAC1250.002009726122.9671787
GAGTACT35550.022.86982712-13
AGTACGC4300.022.7359432
GTACATG47050.022.0672251
ATACCGA1853.5290643E-521.7362486
CCGACCG1904.36459E-521.1454379
TACATGG44450.020.9592482
TAGTACG1955.2950487E-520.6215694
AGTACTT38250.020.35419312-13
GTACCTG19300.020.0240671
ACATGGG45200.019.8264273
Produced by FastQC (version 0.11.5)