##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003813_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1249485 Sequences flagged as poor quality 0 Sequence length 121 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.87975605949651 31.0 31.0 34.0 28.0 34.0 2 30.782346326686596 31.0 31.0 34.0 27.0 34.0 3 30.865226073142136 31.0 31.0 34.0 27.0 34.0 4 34.316007795211625 37.0 35.0 37.0 30.0 37.0 5 34.28345118188694 37.0 35.0 37.0 30.0 37.0 6 34.41230987166713 37.0 35.0 37.0 30.0 37.0 7 34.462253648503186 37.0 35.0 37.0 30.0 37.0 8 34.43644541551119 37.0 35.0 37.0 30.0 37.0 9 35.916141450277514 39.0 35.0 39.0 30.0 39.0 10-11 35.745437920423214 39.0 35.0 39.0 30.0 39.0 12-13 35.61454999459777 39.0 35.0 39.0 30.0 39.0 14-15 36.599009591951884 39.0 36.0 41.0 30.0 41.0 16-17 36.705055282776506 39.0 36.0 41.0 30.5 41.0 18-19 36.707813219046244 39.5 36.0 41.0 30.5 41.0 20-21 36.58252920203124 39.0 36.0 41.0 30.0 41.0 22-23 36.50059624565321 39.0 36.0 41.0 30.0 41.0 24-25 36.29922928246438 39.0 36.0 41.0 29.0 41.0 26-27 36.091278406703566 39.0 36.0 41.0 28.0 41.0 28-29 35.927410893288034 39.0 35.0 40.5 27.0 41.0 30-31 35.72031717067432 38.5 35.0 40.0 27.0 41.0 32-33 35.455374014093806 38.0 35.0 40.0 27.0 41.0 34-35 35.385245521154715 38.0 35.0 40.0 26.0 41.0 36-37 35.2364478165004 38.0 35.0 40.0 25.0 41.0 38-39 35.25501666686675 38.5 35.0 40.0 25.5 41.0 40-41 35.36612964541391 39.0 35.0 40.5 25.5 41.0 42-43 35.21681172643129 39.0 35.0 41.0 25.0 41.0 44-45 34.99591511702822 39.0 35.0 40.0 24.0 41.0 46-47 34.72288943044534 38.0 34.0 40.0 23.5 41.0 48-49 34.458332833127244 38.0 34.0 40.0 22.5 41.0 50-51 33.68949327122775 37.5 33.5 39.5 21.0 40.5 52-53 33.84905981264281 38.0 33.5 40.0 22.0 40.5 54-55 34.18449201070841 38.0 34.0 40.0 22.0 41.0 56-57 33.94220298763091 38.0 33.5 40.0 19.5 41.0 58-59 33.659443690800614 38.0 33.0 40.0 18.0 41.0 60-61 33.35171970851991 38.0 33.0 40.0 15.5 41.0 62-63 33.135797148425155 37.0 32.0 40.0 14.0 41.0 64-65 32.85599987194725 37.0 32.0 40.0 13.0 41.0 66-67 32.449912563976355 36.0 31.5 39.0 9.5 41.0 68-69 32.06484311536353 36.0 31.0 39.0 8.0 41.0 70-71 31.622713758068322 35.5 31.0 39.0 7.0 40.5 72-73 31.252883387955837 35.0 31.0 38.0 4.5 40.0 74-75 30.887238742361852 35.0 30.0 37.0 2.0 40.0 76-77 30.452556453258744 35.0 30.0 37.0 2.0 39.0 78-79 29.854115495584182 34.5 29.0 36.0 2.0 39.0 80-81 29.352854175920477 34.0 29.0 36.0 2.0 38.5 82-83 28.973905248962573 34.0 29.0 35.5 2.0 37.0 84-85 28.71071601499818 34.0 29.0 35.0 2.0 37.0 86-87 28.50906613524772 34.0 29.0 35.0 2.0 36.5 88-89 28.137985650087835 34.0 27.5 35.0 2.0 36.0 90-91 27.73009319839774 34.0 26.0 35.0 2.0 36.0 92-93 27.44510338259363 34.0 26.0 35.0 2.0 35.5 94-95 27.201045230635025 34.0 25.0 35.0 2.0 35.0 96-97 26.9184796136008 34.0 25.0 35.0 2.0 35.0 98-99 26.665181654841795 33.5 25.0 35.0 2.0 35.0 100-101 26.368471810385877 33.0 24.5 35.0 2.0 35.0 102-103 25.977766839938056 33.0 23.5 35.0 2.0 35.0 104-105 25.617748112222237 33.0 20.0 35.0 2.0 35.0 106-107 25.250400765115227 32.0 19.0 35.0 2.0 35.0 108-109 24.95424274801218 32.0 18.0 35.0 2.0 35.0 110-111 24.59100629459337 32.0 13.0 35.0 2.0 35.0 112-113 24.074788012661216 32.0 4.5 34.0 2.0 35.0 114-115 23.55345202223316 31.0 2.0 34.0 2.0 35.0 116-117 22.92943972916842 31.0 2.0 34.0 2.0 35.0 118-119 22.206348615629636 31.0 2.0 34.0 2.0 35.0 120-121 20.599539810401886 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.638599466229337 1101 2 0.4370343186048551 1101 3 0.6010694092112736 1101 4 0.9898506261378444 1101 5 0.840205447385749 1101 6 0.8562798864283181 1101 7 0.6927929963826074 1101 8 0.6342964144073591 1101 9 0.8477761047016017 1101 10-11 0.8146617932569953 1101 12-13 0.9361641935863219 1101 14-15 1.1489600328193745 1101 16-17 1.1350001851437597 1101 18-19 1.0466692803714892 1101 20-21 1.0085480192852216 1101 22-23 0.9452549967580524 1101 24-25 1.104435056715623 1101 26-27 1.330342987235042 1101 28-29 1.1658235299353308 1101 30-31 1.1805559494596096 1101 32-33 1.0927287889492945 1101 34-35 1.0847015197829037 1101 36-37 1.0300318765441432 1101 38-39 1.1685122743681262 1101 40-41 1.0895364377995094 1101 42-43 1.0380473684619318 1101 44-45 1.3281406891188254 1101 46-47 1.4515886964607532 1101 48-49 1.5519232882627634 1101 50-51 1.1062055336536076 1101 52-53 1.193141196866513 1101 54-55 1.3303210448098923 1101 56-57 1.445820859750313 1101 58-59 1.303730169438154 1101 60-61 1.3159212303089163 1101 62-63 1.1493742618311842 1101 64-65 1.3576550355268893 1101 66-67 1.1662145180908041 1101 68-69 1.165809047160561 1101 70-71 0.8766305690380634 1101 72-73 0.7519918093051885 1101 74-75 0.7148611904027753 1101 76-77 0.6875079219741416 1101 78-79 0.8958633966332563 1101 80-81 1.0649064941632567 1101 82-83 1.1581176536686506 1101 84-85 1.120352408296963 1101 86-87 0.7825773344491473 1101 88-89 1.0015217092229385 1101 90-91 0.8923907090229406 1101 92-93 0.9941700979768378 1101 94-95 0.9986852146595169 1101 96-97 0.824289794209573 1101 98-99 0.8302919921671936 1101 100-101 1.0201799140697858 1101 102-103 0.8236486224467434 1101 104-105 0.7863561759050306 1101 106-107 0.6701643973171443 1101 108-109 0.7651857922826899 1101 110-111 0.3995866271607227 1101 112-113 0.6509019978481838 1101 114-115 0.349812668092337 1101 116-117 0.06124293821706672 1101 118-119 -0.10034640889859858 1101 120-121 0.13257031638983463 1102 1 0.7120216314709928 1102 2 0.6831449707992512 1102 3 0.6100631068824462 1102 4 0.8579137140795083 1102 5 0.749987847497998 1102 6 0.7246414803954693 1102 7 0.7224056194733564 1102 8 0.5779908873000537 1102 9 0.6566565901796935 1102 10-11 0.8251266991966517 1102 12-13 0.8990748199193632 1102 14-15 0.9242520563119427 1102 16-17 0.9835737183890032 1102 18-19 0.7858661478465052 1102 20-21 0.8918662095133456 1102 22-23 0.7532781371652462 1102 24-25 0.9278659914315313 1102 26-27 0.9588037484804275 1102 28-29 0.9298290681162413 1102 30-31 0.9697656444401019 1102 32-33 0.9177255046952197 1102 34-35 0.9446320380347615 1102 36-37 0.9294102411850673 1102 38-39 0.7852449814807585 1102 40-41 1.0156293722169067 1102 42-43 0.9610221529564029 1102 44-45 1.0387755509084826 1102 46-47 1.1789292347482814 1102 48-49 1.2284352659889848 1102 50-51 0.8514664065980213 1102 52-53 0.8798735669562134 1102 54-55 0.9857838770268046 1102 56-57 1.0242481594331636 1102 58-59 0.5376103282103415 1102 60-61 0.6451169766668343 1102 62-63 0.5144409407865709 1102 64-65 0.7300540266590616 1102 66-67 0.6975229167710566 1102 68-69 0.5694775761655073 1102 70-71 0.4728564624411504 1102 72-73 0.6573564578005886 1102 74-75 0.7142965050411938 1102 76-77 0.6151662935206055 1102 78-79 0.6802189296900458 1102 80-81 0.7648501631874787 1102 82-83 0.7786284566292281 1102 84-85 0.5410801439774424 1102 86-87 0.5258900085060176 1102 88-89 0.4256361814690983 1102 90-91 0.6941276839463697 1102 92-93 0.5751150965221363 1102 94-95 0.4564331999157929 1102 96-97 0.6468424058965638 1102 98-99 0.9623100648499694 1102 100-101 0.8379787829107777 1102 102-103 0.6988136089279955 1102 104-105 0.8660514383943116 1102 106-107 0.6259315130955265 1102 108-109 0.6055782658040378 1102 110-111 0.36948635252142026 1102 112-113 0.212907683218706 1102 114-115 0.02115168771326381 1102 116-117 -0.04106106059441217 1102 118-119 -0.15932542199916 1102 120-121 0.21730441561350844 1103 1 0.39640068375177506 1103 2 0.5276967138608626 1103 3 0.5309404574819077 1103 4 0.7716103678083783 1103 5 0.7811270667161523 1103 6 0.9260225935768247 1103 7 0.630376156118885 1103 8 0.6928444187866916 1103 9 0.8809001275525077 1103 10-11 0.8580933633554224 1103 12-13 0.7801533332975197 1103 14-15 0.7861602850143612 1103 16-17 0.7735650735233364 1103 18-19 0.6398383603567837 1103 20-21 0.8961842166505178 1103 22-23 0.7192227510156144 1103 24-25 0.5596452405084023 1103 26-27 0.7285984255445399 1103 28-29 0.8300988882410749 1103 30-31 0.7181576404156615 1103 32-33 0.852177581011972 1103 34-35 0.5011921043612375 1103 36-37 0.5220563519544186 1103 38-39 0.3676618262818181 1103 40-41 0.46517455786761275 1103 42-43 0.4550644242842097 1103 44-45 0.4102130474969954 1103 46-47 0.28701513461830785 1103 48-49 0.6531740390966689 1103 50-51 0.6729484552246561 1103 52-53 0.5944532214271234 1103 54-55 0.49970491370257264 1103 56-57 0.5557248382114963 1103 58-59 0.4753565999276219 1103 60-61 0.4345835176436381 1103 62-63 0.5607964512983443 1103 64-65 0.4291131230198033 1103 66-67 0.24540566229777738 1103 68-69 0.29991082260761814 1103 70-71 0.24867746651426614 1103 72-73 0.1919123167624086 1103 74-75 0.18290797143759718 1103 76-77 0.25291212266796137 1103 78-79 0.5520794493556416 1103 80-81 0.37311972957223105 1103 82-83 0.5104838277314769 1103 84-85 0.5058110098359769 1103 86-87 0.3772853576863042 1103 88-89 0.5693260933829336 1103 90-91 0.24571326442844565 1103 92-93 0.12821684777128795 1103 94-95 0.3138991330965908 1103 96-97 0.5143956138645898 1103 98-99 0.46980793682629596 1103 100-101 0.2971992845195217 1103 102-103 0.38604096741995164 1103 104-105 0.08081512818820613 1103 106-107 0.23836603118852295 1103 108-109 0.17534203673574922 1103 110-111 0.1766084826399279 1103 112-113 0.21795519309907974 1103 114-115 0.2232781623931004 1103 116-117 -0.05803066636788401 1103 118-119 -0.014055535488839865 1103 120-121 -0.12472431012443863 1104 1 0.5635915219065701 1104 2 0.6275078287415248 1104 3 0.7431473875485679 1104 4 0.8994470830411387 1104 5 1.1881974218385167 1104 6 1.026721590774109 1104 7 0.8309137757384804 1104 8 0.9670075018319579 1104 9 0.9130656129230843 1104 10-11 1.0371177931087985 1104 12-13 1.0784626892237128 1104 14-15 1.0620288356419962 1104 16-17 1.0558871990616367 1104 18-19 0.9081324037514591 1104 20-21 1.0690538339594866 1104 22-23 1.0076179304717598 1104 24-25 1.0955135466245522 1104 26-27 1.024763721418708 1104 28-29 0.9718505990573476 1104 30-31 1.0737781952733414 1104 32-33 0.9258588236193219 1104 34-35 0.6880653526657028 1104 36-37 0.909484794141207 1104 38-39 0.7458507908673795 1104 40-41 0.928118842870191 1104 42-43 1.0549320824449353 1104 44-45 1.3917447896718755 1104 46-47 1.2754887105681618 1104 48-49 1.1494788593348844 1104 50-51 1.055912270581814 1104 52-53 1.1352751773355934 1104 54-55 1.123264206208603 1104 56-57 1.2202405679407278 1104 58-59 1.0431114955059329 1104 60-61 0.8272506183181889 1104 62-63 0.8554396632011674 1104 64-65 0.9644694832218903 1104 66-67 0.9520744073120397 1104 68-69 0.9331978515791697 1104 70-71 0.996693867121877 1104 72-73 0.8951242807091937 1104 74-75 1.0192067705805847 1104 76-77 0.9597520257191618 1104 78-79 1.1676340472412328 1104 80-81 1.2503344687243256 1104 82-83 1.1136607959724927 1104 84-85 1.0266517931285044 1104 86-87 1.2033681614994585 1104 88-89 1.1123738678699944 1104 90-91 1.1097167426042702 1104 92-93 1.245206422413002 1104 94-95 1.1881484064390335 1104 96-97 1.1038134625914502 1104 98-99 0.9845871276766758 1104 100-101 1.020906659291434 1104 102-103 0.9160371774919902 1104 104-105 0.7937005776155921 1104 106-107 0.8121351451094796 1104 108-109 1.0008999049623988 1104 110-111 1.0337534137640993 1104 112-113 0.836106961375549 1104 114-115 0.8282140851030242 1104 116-117 0.8414392021766304 1104 118-119 0.8540969968860104 1104 120-121 0.5439465865908559 1105 1 0.7450860820281129 1105 2 0.9603892956493283 1105 3 1.0951359706374255 1105 4 1.1825766262057726 1105 5 1.258094218301558 1105 6 1.1628472785063337 1105 7 0.9354157002762591 1105 8 1.1738214144442765 1105 9 1.313806504344825 1105 10-11 1.2858654927219888 1105 12-13 1.3285763180024617 1105 14-15 1.5107163040563734 1105 16-17 1.350271553570714 1105 18-19 1.142841784244176 1105 20-21 1.1739411428168083 1105 22-23 1.2040868565765024 1105 24-25 1.3555414850012042 1105 26-27 1.3269908454822712 1105 28-29 1.3670181180722878 1105 30-31 1.2678903174775158 1105 32-33 1.1957200216957418 1105 34-35 1.3060938695161326 1105 36-37 1.1521616974629865 1105 38-39 0.9940772283906227 1105 40-41 1.004653784911568 1105 42-43 1.0957518306499239 1105 44-45 0.8162944859731311 1105 46-47 1.127037876072059 1105 48-49 1.0007975888538923 1105 50-51 0.7571512215736362 1105 52-53 0.768051597218566 1105 54-55 0.8197156509627774 1105 56-57 0.8902498807329451 1105 58-59 0.952794892354845 1105 60-61 0.9392877995616544 1105 62-63 0.7434716127531686 1105 64-65 0.7963060421292738 1105 66-67 0.7519145324149363 1105 68-69 0.5701936338060385 1105 70-71 0.3095504469433159 1105 72-73 0.20529588400331278 1105 74-75 0.04097105204939666 1105 76-77 0.13750637142399214 1105 78-79 0.2640548201712001 1105 80-81 0.6186474548250622 1105 82-83 0.6512361644316869 1105 84-85 0.7633445123049079 1105 86-87 0.7028318018794728 1105 88-89 0.588623863896963 1105 90-91 0.5865587207303307 1105 92-93 0.7258784055291976 1105 94-95 0.8042070929221339 1105 96-97 0.6140027061575175 1105 98-99 0.7185885134710119 1105 100-101 0.6971194677180854 1105 102-103 0.7797001243391506 1105 104-105 0.8891336542379413 1105 106-107 0.628735912381849 1105 108-109 0.8256668467749293 1105 110-111 0.547710682986434 1105 112-113 0.4400264390963642 1105 114-115 0.7420667840646296 1105 116-117 0.5564343868591557 1105 118-119 0.2833420250309864 1105 120-121 0.31524284462830465 1106 1 0.5081483683769115 1106 2 0.5635088128437431 1106 3 0.6274700565373692 1106 4 0.8684980394654858 1106 5 0.8846294038657021 1106 6 0.873316564731013 1106 7 0.7525789155360272 1106 8 0.6212250596398761 1106 9 0.7799091374333784 1106 10-11 0.9035040661777387 1106 12-13 1.0118617912304444 1106 14-15 1.0293859384968655 1106 16-17 1.0287313192127456 1106 18-19 0.963561799273144 1106 20-21 1.00956097031915 1106 22-23 0.7405321031622734 1106 24-25 0.8927188905585624 1106 26-27 0.9106985052237562 1106 28-29 1.033968814336184 1106 30-31 1.100688858862533 1106 32-33 1.0525506539523306 1106 34-35 1.164822412142378 1106 36-37 1.2356353682547905 1106 38-39 1.3216994981002088 1106 40-41 1.3551172339925586 1106 42-43 1.4493175203218627 1106 44-45 1.2896474218215843 1106 46-47 1.4865430271836289 1106 48-49 1.5579630182026492 1106 50-51 1.4063263056922466 1106 52-53 1.5070214084507043 1106 54-55 1.2738965889984684 1106 56-57 1.393092133602039 1106 58-59 1.7803634871799119 1106 60-61 1.6063148148019692 1106 62-63 1.7097990094206281 1106 64-65 1.5502142446580365 1106 66-67 1.3385214745903156 1106 68-69 1.2527024985486825 1106 70-71 1.16111339248679 1106 72-73 1.158260750436714 1106 74-75 0.9785194852459718 1106 76-77 1.0286780270593532 1106 78-79 1.1984875940556847 1106 80-81 0.9433301859373735 1106 82-83 0.8993466407627366 1106 84-85 1.2574771764913528 1106 86-87 1.0234822072925844 1106 88-89 1.024103298029491 1106 90-91 0.7785890067839176 1106 92-93 0.7586544705563192 1106 94-95 0.9990033128683891 1106 96-97 0.9509417616808342 1106 98-99 1.0516428492275445 1106 100-101 0.9714887039580233 1106 102-103 0.7618180010949054 1106 104-105 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0.9758167363278929 2204 72-73 0.7725920007376885 2204 74-75 1.024393735286413 2204 76-77 0.7963121863205735 2204 78-79 0.9050961284380641 2204 80-81 1.066170753085224 2204 82-83 0.8427880522363296 2204 84-85 0.6528782682620751 2204 86-87 0.8416836131850864 2204 88-89 0.8838958961647627 2204 90-91 0.9487222492392533 2204 92-93 0.8990359729326087 2204 94-95 0.9433535043172157 2204 96-97 0.9834287936622346 2204 98-99 1.0080309797708225 2204 100-101 0.994792551332381 2204 102-103 0.9626540539770083 2204 104-105 0.8699885885469705 2204 106-107 1.133696832407015 2204 108-109 1.1103341850813067 2204 110-111 1.2851274402759643 2204 112-113 1.3330702399467036 2204 114-115 1.4914436381963654 2204 116-117 1.3287761059900944 2204 118-119 1.284625596408464 2204 120-121 1.2911274632302998 2205 1 1.0117863678135564 2205 2 1.0731321341578521 2205 3 1.2132993760661108 2205 4 1.180596629810772 2205 5 1.356892460934361 2205 6 1.20792212347083 2205 7 1.1295392801207527 2205 8 1.2022637393095366 2205 9 1.393256783594488 2205 10-11 1.4375777286593987 2205 12-13 1.4973090927906014 2205 14-15 1.6507838495900984 2205 16-17 1.6647225689552485 2205 18-19 1.6231058021095492 2205 20-21 1.731066928313986 2205 22-23 0.7919896152247148 2205 24-25 1.4454758382826398 2205 26-27 1.615502738532193 2205 28-29 1.6292235093314105 2205 30-31 -4.244604023915688 2205 32-33 -2.071895272847648 2205 34-35 0.9649335420943501 2205 36-37 1.7046402016369342 2205 38-39 1.755970966290839 2205 40-41 1.8957942298122248 2205 42-43 1.7214681861906769 2205 44-45 1.8345306793165435 2205 46-47 1.6681238332331318 2205 48-49 1.7058734107450348 2205 50-51 1.7711044171557262 2205 52-53 1.787491405465751 2205 54-55 1.5982425969387322 2205 56-57 1.7308168284150867 2205 58-59 1.6776758380198302 2205 60-61 1.701894281125952 2205 62-63 1.6231579367433255 2205 64-65 1.635099730357588 2205 66-67 1.6546707727058703 2205 68-69 1.709030389363857 2205 70-71 1.7954727276129603 2205 72-73 1.7016214014648376 2205 74-75 1.7316743566757644 2205 76-77 1.7346156317254078 2205 78-79 2.001916506621779 2205 80-81 1.9200668191622832 2205 82-83 1.800142488174167 2205 84-85 1.797712034860222 2205 86-87 1.8872597287723387 2205 88-89 1.728200882179248 2205 90-91 1.7066285090674782 2205 92-93 1.7879662216028045 2205 94-95 1.7447308356794622 2205 96-97 1.7818469592833424 2205 98-99 1.8221836723179088 2205 100-101 1.9095057280016121 2205 102-103 1.9723314831340346 2205 104-105 2.2392823947593428 2205 106-107 1.9022650530028713 2205 108-109 1.8112090204229538 2205 110-111 2.09127954737113 2205 112-113 2.1580097322748024 2205 114-115 2.172430833714518 2205 116-117 2.328215723157207 2205 118-119 2.4616982152372238 2205 120-121 2.5299128429358824 2206 1 0.7813310161703733 2206 2 1.0695870263482696 2206 3 1.0880942537514038 2206 4 1.2367894004860105 2206 5 1.3051928836202933 2206 6 1.2460880050179668 2206 7 1.0404616819445565 2206 8 1.2053233976061364 2206 9 1.1843580430574363 2206 10-11 1.3131861985066209 2206 12-13 1.4650890393221871 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1.6294138709982136 2206 82-83 1.508760304648785 2206 84-85 1.4706554134084477 2206 86-87 1.4250640432778319 2206 88-89 1.3762823485657165 2206 90-91 1.5834795174495753 2206 92-93 1.6342713141538887 2206 94-95 1.5049198373565176 2206 96-97 1.4357397603588637 2206 98-99 1.625961265347808 2206 100-101 1.4384533742611865 2206 102-103 1.4277038176050354 2206 104-105 1.4418023817503567 2206 106-107 1.3664022712763568 2206 108-109 1.3545328693637977 2206 110-111 1.3939384843374967 2206 112-113 1.4608702766891888 2206 114-115 1.4364830054776974 2206 116-117 1.3428495058806185 2206 118-119 1.5919199144590728 2206 120-121 1.1451195621937984 2207 1 0.8354871348469324 2207 2 1.0347553240535028 2207 3 1.0049997274298583 2207 4 1.2348029662025937 2207 5 1.2053188664591943 2207 6 1.1576674420195872 2207 7 1.091882070124342 2207 8 1.2536615122365973 2207 9 1.3457749090809017 2207 10-11 1.3859886024427723 2207 12-13 1.4067710520359853 2207 14-15 1.5746942392636143 2207 16-17 1.3793307304942957 2207 18-19 1.3170363551121724 2207 20-21 1.6141266031642445 2207 22-23 1.5933422734710874 2207 24-25 1.6836506240088625 2207 26-27 1.5859893569312717 2207 28-29 1.5842853765037361 2207 30-31 1.6510905550037123 2207 32-33 1.5880140620083196 2207 34-35 1.7499298239742345 2207 36-37 1.7752744274494603 2207 38-39 1.6935333656208513 2207 40-41 1.7286268786501608 2207 42-43 1.8474169213873353 2207 44-45 1.8450229852382165 2207 46-47 1.915594757310295 2207 48-49 1.8120535353677028 2207 50-51 1.6909611899168553 2207 52-53 1.6235541537012494 2207 54-55 1.734764928286637 2207 56-57 1.642213550994164 2207 58-59 1.6400848334681442 2207 60-61 1.7728572993790905 2207 62-63 1.4811506100074396 2207 64-65 1.6362724327074005 2207 66-67 1.6128864773077893 2207 68-69 1.6314124668516037 2207 70-71 1.6622684902410185 2207 72-73 1.596967354244999 2207 74-75 1.5808941101456817 2207 76-77 1.634902410246685 2207 78-79 1.4029882526521043 2207 80-81 1.412744273498685 2207 82-83 1.590397493668938 2207 84-85 1.5822043731862472 2207 86-87 1.5167355101502267 2207 88-89 1.553873286464892 2207 90-91 1.6820607021214364 2207 92-93 1.5987323443046826 2207 94-95 1.7254957795333326 2207 96-97 1.618798033739047 2207 98-99 1.4881897743927368 2207 100-101 1.5907740008440285 2207 102-103 1.8382431097599117 2207 104-105 1.9558512989838057 2207 106-107 1.9821845225994856 2207 108-109 2.0590791326034683 2207 110-111 2.0336724300344073 2207 112-113 2.126100663358006 2207 114-115 2.071275618541236 2207 116-117 2.126464826590226 2207 118-119 2.2889528458471595 2207 120-121 1.737697094755692 2208 1 0.6966512502330957 2208 2 0.9721699442350875 2208 3 0.8435724108951028 2208 4 1.0201661304507752 2208 5 1.0584786408768707 2208 6 0.9800039454500151 2208 7 1.0995593145454663 2208 8 0.9816918903079568 2208 9 1.2116865505613177 2208 10-11 1.1744251680269926 2208 12-13 1.2175220243454419 2208 14-15 1.2546403363650285 2208 16-17 1.2389143327797214 2208 18-19 1.088602412831385 2208 20-21 1.3092644572149652 2208 22-23 1.1999765642920295 2208 24-25 1.344354627213427 2208 26-27 1.3035763205283075 2208 28-29 1.3716176510819693 2208 30-31 1.3085039995970433 2208 32-33 1.3968696703806884 2208 34-35 1.1010584431260853 2208 36-37 1.257247924465318 2208 38-39 1.3766179023900946 2208 40-41 1.2414054141502078 2208 42-43 1.3379735733119773 2208 44-45 1.2999612178718962 2208 46-47 1.0610071329891113 2208 48-49 1.1752822776297691 2208 50-51 1.0840095991173513 2208 52-53 0.9783741744046637 2208 54-55 0.972788350794147 2208 56-57 1.0098591851532746 2208 58-59 0.944664783810417 2208 60-61 0.8886875749130354 2208 62-63 0.925573042408395 2208 64-65 0.974089419740011 2208 66-67 0.8659250463991768 2208 68-69 1.074565971465244 2208 70-71 0.8707558020516544 2208 72-73 0.8864918832205255 2208 74-75 0.8651325738695341 2208 76-77 0.9700607173560591 2208 78-79 0.8034869524297221 2208 80-81 0.8646115539385342 2208 82-83 1.0097001630162374 2208 84-85 0.7649623576619469 2208 86-87 1.0470776607583225 2208 88-89 1.081992976588534 2208 90-91 1.2039979409476338 2208 92-93 1.2217848680640806 2208 94-95 1.2393652718207093 2208 96-97 1.0919513533063885 2208 98-99 0.993701977107694 2208 100-101 1.1896374355863628 2208 102-103 1.3152913818084464 2208 104-105 1.4645008528589507 2208 106-107 1.4427936079373964 2208 108-109 1.443858693477715 2208 110-111 1.5213687559945512 2208 112-113 1.7775382009795528 2208 114-115 1.8791017708082158 2208 116-117 1.8672878907610162 2208 118-119 1.8988913016249036 2208 120-121 1.7797420631835301 2209 1 0.4664466935883631 2209 2 0.6872707983516548 2209 3 0.8880106837810722 2209 4 0.9670656344202371 2209 5 1.0418795222568065 2209 6 0.9367894722637402 2209 7 0.834874735438973 2209 8 0.8180445399411198 2209 9 0.9814006107279099 2209 10-11 1.003505721107139 2209 12-13 1.1145539794544703 2209 14-15 1.3314742047928263 2209 16-17 0.9526230257250248 2209 18-19 0.9985005414249528 2209 20-21 1.1059580617170823 2209 22-23 0.9591896498949382 2209 24-25 1.0323981143202943 2209 26-27 1.148268712420915 2209 28-29 1.3272719515731097 2209 30-31 1.072786749209456 2209 32-33 1.0437998965038773 2209 34-35 0.9904775095795273 2209 36-37 0.94360525363183 2209 38-39 0.7993267621709279 2209 40-41 0.8885894540930721 2209 42-43 0.9810711598935242 2209 44-45 0.9873083700706644 2209 46-47 0.9218174653987532 2209 48-49 0.9763985497914334 2209 50-51 0.9659114021619004 2209 52-53 1.0021126332512225 2209 54-55 0.7328244238925308 2209 56-57 0.8643765624986344 2209 58-59 0.614460634081631 2209 60-61 0.6793659444822353 2209 62-63 0.7428454034567693 2209 64-65 0.7763323404198914 2209 66-67 0.9064238643083726 2209 68-69 0.9424006903473661 2209 70-71 0.850463412600547 2209 72-73 0.7582299432894608 2209 74-75 0.8156063257566082 2209 76-77 0.5885495536171526 2209 78-79 0.5970544251968697 2209 80-81 0.5977331892523239 2209 82-83 0.586517246161371 2209 84-85 0.9607260575044734 2209 86-87 0.8257550618675715 2209 88-89 0.7871065875199221 2209 90-91 0.9556403554240767 2209 92-93 0.9814811663497451 2209 94-95 1.0335690593943134 2209 96-97 0.9222389208035686 2209 98-99 1.0588888939462784 2209 100-101 1.0499559083494425 2209 102-103 1.283555498739812 2209 104-105 1.4242644957332402 2209 106-107 1.333620094909982 2209 108-109 1.2351133577517182 2209 110-111 1.2533973133554568 2209 112-113 1.3768099231698976 2209 114-115 1.2723366915206462 2209 116-117 1.124726822850814 2209 118-119 1.4300493640539464 2209 120-121 1.4387429421219657 2210 1 0.4115418165975946 2210 2 0.5611053513551951 2210 3 0.6409880311532881 2210 4 0.6787953126864466 2210 5 0.7661875777137723 2210 6 0.7623484643034928 2210 7 0.5668993879878883 2210 8 0.6576959339706505 2210 9 0.7545729363263334 2210 10-11 0.7952723838303015 2210 12-13 0.7921948612225123 2210 14-15 1.0488656316098002 2210 16-17 0.7590440401075611 2210 18-19 0.7284090802431464 2210 20-21 0.9502247666920951 2210 22-23 0.8011106727278374 2210 24-25 0.9600073071971948 2210 26-27 1.0061433314500263 2210 28-29 1.1354626144765447 2210 30-31 1.0993617575189845 2210 32-33 1.0885924962323372 2210 34-35 1.0986922560402306 2210 36-37 0.8250057054450011 2210 38-39 1.0494499967475903 2210 40-41 1.0972396763378356 2210 42-43 1.0468016662960835 2210 44-45 1.2401603702734718 2210 46-47 1.1780989078207327 2210 48-49 0.8366413852852403 2210 50-51 0.6393524495572152 2210 52-53 1.1464139847472197 2210 54-55 1.1735950695572086 2210 56-57 0.9636994061048014 2210 58-59 0.7435527262244719 2210 60-61 0.881296009212079 2210 62-63 1.0327804482719074 2210 64-65 1.1061742621469222 2210 66-67 1.3062369702703833 2210 68-69 1.1865628924013656 2210 70-71 1.2531341523460213 2210 72-73 1.1505271484700934 2210 74-75 1.1194133587186847 2210 76-77 1.080828731339718 2210 78-79 0.7737502738112916 2210 80-81 0.5023573730873281 2210 82-83 0.8362644392298151 2210 84-85 0.9501947826815105 2210 86-87 1.0963401655536238 2210 88-89 1.4156895448117481 2210 90-91 1.465784321577523 2210 92-93 1.3541418219858592 2210 94-95 1.303154906713008 2210 96-97 1.4145364076632418 2210 98-99 1.2784675584496519 2210 100-101 1.4172407196526606 2210 102-103 1.3392497812188147 2210 104-105 1.4388632157837158 2210 106-107 1.6130372446561907 2210 108-109 1.7667438004948188 2210 110-111 1.7261307830214427 2210 112-113 1.7991052411010209 2210 114-115 1.8374362368906851 2210 116-117 1.397837883259701 2210 118-119 1.7852642288198446 2210 120-121 1.8080952775228276 2211 1 0.42103228459779274 2211 2 0.636590302848763 2211 3 0.4759943737816208 2211 4 0.7563473741535134 2211 5 0.6781035931888795 2211 6 0.6943396884533328 2211 7 0.5467533043915935 2211 8 0.7302113616804888 2211 9 0.8433494184924939 2211 10-11 0.8772478078383941 2211 12-13 0.9517395011372827 2211 14-15 0.9273447099649559 2211 16-17 0.8474194243895781 2211 18-19 0.686756520845897 2211 20-21 0.8478687398408837 2211 22-23 0.710544711650364 2211 24-25 0.8824888774604247 2211 26-27 0.978870029626691 2211 28-29 0.9346543243678909 2211 30-31 1.076899824574788 2211 32-33 0.8097258629716819 2211 34-35 0.7420916339660835 2211 36-37 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0.3289235821735872 2312 16-17 0.23225591427729597 2312 18-19 0.21709112751126014 2312 20-21 0.1753316555332134 2312 22-23 0.182264245396631 2312 24-25 0.2801602863963595 2312 26-27 0.0824361030634364 2312 28-29 0.19185876555958004 2312 30-31 0.17534906682323026 2312 32-33 0.1403068058323953 2312 34-35 0.1781923818998976 2312 36-37 0.08812290007915635 2312 38-39 0.2090655368325116 2312 40-41 -0.05986624972283039 2312 42-43 0.25821190079926737 2312 44-45 0.36383057570951394 2312 46-47 0.1337391200734075 2312 48-49 0.055519828741182664 2312 50-51 0.006927418735379831 2312 52-53 -0.018773141922714842 2312 54-55 -0.09891244986835801 2312 56-57 -0.1982195035279588 2312 58-59 -0.1031674353903469 2312 60-61 -0.29822143383413646 2312 62-63 -0.21583785153866586 2312 64-65 -0.10178539661723107 2312 66-67 0.06796144051146769 2312 68-69 0.08406512939602351 2312 70-71 0.02220533562891802 2312 72-73 -0.009065654194817085 2312 74-75 0.07761968403926289 2312 76-77 -0.1243411148910134 2312 78-79 -0.042868759108817045 2312 80-81 -0.20477455172025216 2312 82-83 0.060534866619814665 2312 84-85 0.07834150380774219 2312 86-87 0.1576664323724266 2312 88-89 0.11746140994164023 2312 90-91 0.3203617546651998 2312 92-93 0.3752674115510857 2312 94-95 0.26862916795356284 2312 96-97 0.1695620664972104 2312 98-99 0.37669665793757545 2312 100-101 0.37584621301333243 2312 102-103 0.16983188673146898 2312 104-105 0.4839600174785055 2312 106-107 0.4225670256236356 2312 108-109 0.6925194450891929 2312 110-111 0.43949344992329387 2312 112-113 0.4657466439676803 2312 114-115 0.3412285629425327 2312 116-117 0.45093641942919405 2312 118-119 0.6839894634057124 2312 120-121 0.7827400684947357 2313 1 -0.7928976026160015 2313 2 -0.4548953558995166 2313 3 -0.3495305680170837 2313 4 -0.09539640452334197 2313 5 -2.521930310479494 2313 6 -7.645725406589953 2313 7 -3.0598036650725007 2313 8 -1.282251626814869 2313 9 -0.7388647170769786 2313 10-11 -0.3471526622924799 2313 12-13 -0.3691643706616432 2313 14-15 0.09633754498970859 2313 16-17 -0.17974306459173306 2313 18-19 -0.15171171141683004 2313 20-21 -0.061504494343054716 2313 22-23 -0.1662357960005707 2313 24-25 0.1721448682386324 2313 26-27 0.25523239203430137 2313 28-29 0.041811388762248214 2313 30-31 -0.06663022537661334 2313 32-33 -0.09311752468286016 2313 34-35 0.03190019125197807 2313 36-37 0.09075829192666163 2313 38-39 0.05000253949113187 2313 40-41 -0.22726181378270383 2313 42-43 -0.09298815807674998 2313 44-45 -0.24064883148255944 2313 46-47 -0.027054280292730937 2313 48-49 -0.15806374300937165 2313 50-51 -0.4331013753181452 2313 52-53 -0.2251469310545744 2313 54-55 -0.23056865990855613 2313 56-57 -0.27247504385344 2313 58-59 -0.37284697505347 2313 60-61 -0.18279522664573733 2313 62-63 -0.28398730463563027 2313 64-65 -0.039871741980277875 2313 66-67 -0.08718998125242194 2313 68-69 -0.9270001125936176 2313 70-71 -0.665039036651514 2313 72-73 -0.16793615454899324 2313 74-75 -0.17195208490915448 2313 76-77 -0.09271296295408504 2313 78-79 -0.13824630158694262 2313 80-81 -0.32808761575712353 2313 82-83 -0.21744504193836534 2313 84-85 0.0010745470540207691 2313 86-87 0.006839408279258663 2313 88-89 -0.12746881277530164 2313 90-91 -0.1618360687619358 2313 92-93 -0.5139449376941059 2313 94-95 -0.04928547101684444 2313 96-97 0.04140185012783704 2313 98-99 0.18976874426454415 2313 100-101 0.11091102263220165 2313 102-103 0.2800581454363815 2313 104-105 0.29058586957090426 2313 106-107 0.3330860545372829 2313 108-109 0.3920518866961409 2313 110-111 0.5102801347434003 2313 112-113 0.6631850771296008 2313 114-115 0.46671727990526435 2313 116-117 0.4587655501799546 2313 118-119 0.4801882803656845 2313 120-121 0.3943933807308291 2314 1 -0.5193716606510463 2314 2 -0.15436520476760407 2314 3 -0.19602996844139753 2314 4 -0.6141075352696745 2314 5 -0.18390313295169136 2314 6 -0.4110233253098201 2314 7 -0.4257458242246486 2314 8 -0.23043129561605724 2314 9 -0.1472961591171824 2314 10-11 -2.668921871774998 2314 12-13 -1.0530639429481425 2314 14-15 -0.17985857209018974 2314 16-17 -0.24156392982556696 2314 18-19 -0.34129026068641366 2314 20-21 -0.0921598038648952 2314 22-23 -0.10835489552042077 2314 24-25 0.1460575001088742 2314 26-27 0.09357003810501396 2314 28-29 -0.017503881240997998 2314 30-31 -0.17459754243321157 2314 32-33 -0.2903250069990193 2314 34-35 -0.0440434729079584 2314 36-37 -0.15170841584451722 2314 38-39 -0.18850235663904868 2314 40-41 -0.04610159842094674 2314 42-43 -0.10587175300633334 2314 44-45 0.1171111828878395 2314 46-47 0.043491988987149455 2314 48-49 0.09363861499800663 2314 50-51 0.04179339550800876 2314 52-53 0.06976347243315928 2314 54-55 -0.03785289762210908 2314 56-57 -0.107861660935761 2314 58-59 -0.050920710725549156 2314 60-61 0.04327563494695141 2314 62-63 0.14695177167797624 2314 64-65 0.2274754147687048 2314 66-67 0.2617492309973741 2314 68-69 0.3803545206456924 2314 70-71 0.33490974453491873 2314 72-73 0.21330343783663963 2314 74-75 0.2174315415770387 2314 76-77 0.4415849509109222 2314 78-79 0.38236550835484806 2314 80-81 0.3260713033559881 2314 82-83 0.35626878325740563 2314 84-85 0.42507747939177776 2314 86-87 0.43480560871716634 2314 88-89 0.5651039737708992 2314 90-91 0.673182549640913 2314 92-93 0.7291157626685525 2314 94-95 0.8003688626773666 2314 96-97 0.7793409118768508 2314 98-99 0.8392536432105508 2314 100-101 0.8460624215612214 2314 102-103 0.9030262635455095 2314 104-105 1.1444801986044446 2314 106-107 1.0108544192734925 2314 108-109 1.1706047974297569 2314 110-111 1.0156068913545084 2314 112-113 0.9747789774786675 2314 114-115 1.1106227265770166 2314 116-117 1.1214513094078349 2314 118-119 1.3316326023596723 2314 120-121 1.2430277075311373 2315 1 -0.4724810453393786 2315 2 -3.0099516761435474 2315 3 -0.9411344135458428 2315 4 -0.334196739569407 2315 5 -3.091488624747708 2315 6 -0.9198781025856491 2315 7 -0.597290069453102 2315 8 -0.2772359371575348 2315 9 -0.14740089080086705 2315 10-11 -0.11409561694873815 2315 12-13 0.09442562296247559 2315 14-15 -0.1483035931667871 2315 16-17 0.020964532702897998 2315 18-19 0.12238347667152283 2315 20-21 0.09172532475707129 2315 22-23 0.030201214088314998 2315 24-25 0.1650313901353897 2315 26-27 0.07148337654467696 2315 28-29 0.21996713517738442 2315 30-31 0.13885552891459696 2315 32-33 0.1457615711928213 2315 34-35 0.13688337643994686 2315 36-37 0.18266467905489492 2315 38-39 0.20167214406176726 2315 40-41 0.0022734973225766453 2315 42-43 -0.13956956301992562 2315 44-45 0.009640583311970374 2315 46-47 -0.1830882349614953 2315 48-49 0.11877057170890737 2315 50-51 -0.20452119554342119 2315 52-53 -0.03979394501899236 2315 54-55 -0.25944012973565833 2315 56-57 -0.2002376112064468 2315 58-59 -0.3310287250440851 2315 60-61 -0.5096026626681933 2315 62-63 -0.5322764443923518 2315 64-65 -0.11054896009777337 2315 66-67 -0.08965657040842956 2315 68-69 -0.2737740440091869 2315 70-71 -0.358081830035605 2315 72-73 -0.3707077856482677 2315 74-75 -0.4116262901123733 2315 76-77 -0.2544589530277186 2315 78-79 -0.5440696022907936 2315 80-81 -0.4585930602557404 2315 82-83 -0.45707174060943245 2315 84-85 -0.350185001133859 2315 86-87 -0.2736591602739189 2315 88-89 -0.22319447505386592 2315 90-91 -0.07645557894458577 2315 92-93 -0.21150085163227317 2315 94-95 0.10053012862810817 2315 96-97 0.09091115765762581 2315 98-99 0.04467129336504527 2315 100-101 0.01802950142303672 2315 102-103 0.16891786364763206 2315 104-105 0.23553580018636566 2315 106-107 0.27380415327585794 2315 108-109 0.3424900719466102 2315 110-111 0.5233606833188276 2315 112-113 0.3150209545627476 2315 114-115 0.43174622138472074 2315 116-117 0.5782119740632368 2315 118-119 0.3832499513454444 2315 120-121 0.2082877912122747 2316 1 -1.7409842164222162 2316 2 -3.3374964631724033 2316 3 -5.67439604316559 2316 4 -2.495878052692298 2316 5 -3.5994471811129394 2316 6 -1.4168643232441056 2316 7 -1.2646165214533056 2316 8 -1.4794259575569697 2316 9 -1.0709347166108643 2316 10-11 -0.8465148668945446 2316 12-13 -0.7982072373486844 2316 14-15 -5.085464181693084 2316 16-17 -2.0046673215329065 2316 18-19 -1.0770412260915663 2316 20-21 -0.6781218740734332 2316 22-23 -1.1153248076895963 2316 24-25 -0.8290869599010122 2316 26-27 -1.1651747301437894 2316 28-29 -0.9505228957830596 2316 30-31 -0.6643180552149772 2316 32-33 -1.3061616446909383 2316 34-35 -0.9931916067458175 2316 36-37 -1.4017380957120693 2316 38-39 -1.2983831622909605 2316 40-41 -1.3271942276686701 2316 42-43 -1.3724023599650153 2316 44-45 -1.8584498745356939 2316 46-47 -1.5893000555132275 2316 48-49 -1.6825956897484033 2316 50-51 -1.4054438944934375 2316 52-53 -1.833925357359817 2316 54-55 -1.773817558073631 2316 56-57 -1.8643885336274764 2316 58-59 -2.134409638516054 2316 60-61 -1.9207356369271125 2316 62-63 -1.8069953269261028 2316 64-65 -1.599877335313856 2316 66-67 -1.4584451667387555 2316 68-69 -1.6429517302581935 2316 70-71 -1.6402401118145207 2316 72-73 -1.600520688981657 2316 74-75 -1.360558516206968 2316 76-77 -1.5904720042152753 2316 78-79 -1.3131892029733656 2316 80-81 -1.4326518795345926 2316 82-83 -1.2684298311784516 2316 84-85 -1.2376696459781407 2316 86-87 -1.2775873009243668 2316 88-89 -1.2765798352677535 2316 90-91 -1.220716780565425 2316 92-93 -1.2400608854275141 2316 94-95 -1.3438283454723994 2316 96-97 -1.1452803190497853 2316 98-99 -1.1149773028541148 2316 100-101 -0.9375308714362731 2316 102-103 -0.994075794186692 2316 104-105 -1.1479048107959748 2316 106-107 -1.0127766912716005 2316 108-109 -1.021049979302088 2316 110-111 -1.030492418561586 2316 112-113 -1.0786766225187456 2316 114-115 -0.7979535679508807 2316 116-117 -0.5989012010772505 2316 118-119 -0.5842363054427828 2316 120-121 -0.9830346698370498 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18782.0 3 2631.0 4 2256.0 5 2536.0 6 2921.0 7 3086.0 8 3953.0 9 5003.0 10 6631.0 11 8065.0 12 8539.0 13 9733.0 14 11908.0 15 12230.0 16 9429.0 17 9311.0 18 9798.0 19 10746.0 20 11655.0 21 12912.0 22 14285.0 23 15921.0 24 17824.0 25 20047.0 26 22555.0 27 25874.0 28 29871.0 29 35087.0 30 41246.0 31 48821.0 32 59607.0 33 73936.0 34 95059.0 35 124544.0 36 171902.0 37 204041.0 38 86267.0 39 473.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.14872825977682 19.371388358298987 13.911169196742737 21.568714185181463 2 13.844792331751945 21.826300603729056 39.51531952380567 24.81358754071332 3 20.886168550506383 25.67041817927558 31.14911393653388 22.294299333684155 4 11.152118943005398 16.633385403492554 42.43534801480161 29.779147638700437 5 10.788395156916895 38.619958927060374 36.69998892760187 13.89165698842086 6 26.32011173906858 38.15181123339757 19.173224655018558 16.354852372515293 7 24.493700751772774 33.161307942417956 24.86894073082915 17.476050574980125 8 22.087909868349296 32.66068535926221 21.919943122371734 23.331461650016763 9 22.010361790151535 19.502155811400232 25.67964408309144 32.80783831535679 10-11 23.25300543004994 25.25793141108596 29.573657876157377 21.915405282706722 12-13 26.43005492335046 23.620837429629205 28.788390404125675 21.160717242894663 14-15 22.0881274492301 25.793992837806947 29.84863747880464 22.269242234158312 16-17 19.278911943914835 29.326009164100096 30.3501322980591 21.044946593925964 18-19 18.522804062246394 30.842510354460423 30.644191317449486 19.990494265843697 20-21 20.205475576993333 27.937784293884306 29.198416521455194 22.658323607667167 22-23 19.067349977072606 27.812888225419712 29.96955807700776 23.150203720499917 24-25 21.45158071550514 27.5140401664702 29.510325208309933 21.52405390971472 26-27 20.741302715239037 25.87017284890234 32.58456515495078 20.803959280907847 28-29 18.852009573186674 29.40203141307945 30.973897590500194 20.772061423233687 30-31 19.761535977913834 27.673779666064704 30.16364599577075 22.401038360250716 32-33 20.227507179301305 27.5256856752988 30.93986923509277 21.306937910307123 34-35 21.879623317218613 26.408206101394637 30.50814512058561 21.20402546080114 36-37 21.385901644777125 26.13880921482575 32.07911516592256 20.39617397447457 38-39 20.723031683551604 27.885259210139772 31.456088861195198 19.93562024511343 40-41 19.880198284690323 28.771042287855508 30.817782900410684 20.53097652704349 42-43 19.201305725804854 28.961598035107343 28.939615030717615 22.89748120837019 44-45 20.057882079908463 27.65556162506182 28.865121743880483 23.421434551149236 46-47 21.508958598298943 28.38599785711169 29.221013233870565 20.884030310718803 48-49 21.144119807453464 27.627090977997103 29.737563625285752 21.491225589263678 50-51 18.9212585505508 28.368349201140315 31.680300937357792 21.03009131095109 52-53 19.6566205573522 29.396991015714015 29.304586527288212 21.641801899645575 54-55 19.03232285108018 28.18133803599492 29.910972441503304 22.87536667142159 56-57 20.956962600592508 26.90471251861439 29.497444830041474 22.64088005075163 58-59 22.46633808025511 26.697140732387993 29.032205914151245 21.804315273205656 60-61 21.009460789564635 27.32719052344582 31.303511264625218 20.35983742236433 62-63 19.824043460586367 28.043497762558882 30.855109019186955 21.27734975766779 64-65 19.286527842367526 30.21761817970984 29.44138296896635 21.05447100895628 66-67 19.690829992860458 28.004375039647282 28.475617073321512 23.82917789417075 68-69 19.270848066675665 28.537194297459006 29.190526262191124 23.0014313736742 70-71 21.335679750433894 27.74842960445631 28.48896530683579 22.42692533827401 72-73 20.333983686744105 26.371925099977695 31.167581040931854 22.126510172346347 74-75 19.259428395919883 29.53031797093484 29.702397310098444 21.50785632304683 76-77 19.889422371538082 27.633696145546317 29.254043593243438 23.22283788967216 78-79 20.335532463119744 27.719956799436936 29.77388469332859 22.170626044114734 80-81 21.385033301190134 27.47798366240658 28.946076715550497 22.19090632085279 82-83 20.278355910856867 26.912645327556767 31.061371444009712 21.747627317576658 84-85 20.141732729102912 27.77221698933164 30.023649072026583 22.062401209538862 86-87 20.486504358426522 28.495532441034122 29.1958798055664 21.822083394972953 88-89 19.636930814844337 27.33285127816698 28.51892524601587 24.51129266097281 90-91 19.8578478374406 25.65497682134732 29.641470627707346 24.845704713504738 92-93 20.751265399926197 26.853316271367706 30.016785773036787 22.378632555669313 94-95 19.743429944193323 26.107969425960775 30.789336269165357 23.35926436068054 96-97 17.56869985719823 27.127162286752256 32.108975574147586 23.195162281901926 98-99 18.648771807388602 28.140694577482417 29.82888071140597 23.381652903723012 100-101 17.82477304748063 27.087144511801934 30.646220894387994 24.441861546329445 102-103 19.37843502919396 25.823659272588234 30.541577645907147 24.25632805231066 104-105 20.8216082260241 26.07376926282063 29.42311865828804 23.68150385286723 106-107 19.4812758149648 26.306813129965974 31.57254055626955 22.63937049879968 108-109 19.22793248450631 26.278625684258174 30.925094821158304 23.56834701007721 110-111 19.154530343804442 27.386309492297105 29.210789105820705 24.248371058077744 112-113 20.13644083347008 25.555273532386003 28.20850978867393 26.099775845469985 114-115 19.34520597665971 25.735027531323624 29.242335872043512 25.67743061997315 116-117 20.9202153616388 25.75901959270177 28.271895484027993 25.04886956163144 118-119 20.252559677290247 25.37361221950279 29.72281987779527 24.651008225411694 120-121 18.940149646094103 27.962729133455987 28.46699249506084 24.63012872538907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3371.0 1 3688.0 2 2462.0 3 899.5 4 819.5 5 793.0 6 833.5 7 897.5 8 1015.0 9 1073.0 10 1056.0 11 1020.0 12 1043.5 13 1114.0 14 1237.0 15 1372.0 16 1500.0 17 1647.5 18 1762.0 19 1911.0 20 2158.5 21 2490.0 22 2871.0 23 3531.0 24 4482.0 25 5533.0 26 6997.0 27 8985.5 28 11896.0 29 15686.0 30 19696.5 31 23591.5 32 27537.0 33 30921.5 34 33279.5 35 35410.5 36 37262.0 37 39253.0 38 40670.0 39 40897.5 40 41281.5 41 41470.0 42 41557.0 43 42788.5 44 43352.5 45 44125.0 46 47947.0 47 63516.0 48 98132.0 49 96810.0 50 61390.5 51 42331.0 52 31804.0 53 25922.0 54 22080.5 55 18707.0 56 15316.0 57 12116.5 58 9593.0 59 7653.0 60 6087.0 61 4614.0 62 3353.5 63 2303.5 64 1527.0 65 1004.5 66 621.0 67 371.0 68 222.0 69 138.5 70 90.5 71 59.5 72 41.5 73 26.5 74 27.5 75 24.0 76 14.5 77 11.5 78 11.5 79 12.0 80 10.5 81 7.5 82 7.0 83 5.5 84 1.5 85 1.0 86 1.0 87 1.5 88 3.0 89 2.0 90 1.0 91 2.0 92 2.0 93 2.0 94 1.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48900146860506527 2 0.9744014533988004 3 0.9911283448780898 4 0.9645573976478309 5 0.9743214204252152 6 0.985766135647887 7 0.9505516272704354 8 0.9395871098892743 9 0.933824735791146 10-11 0.9251011416703682 12-13 0.9266217681684854 14-15 0.9280623616930175 16-17 0.9554736551459201 18-19 0.9873667951195892 20-21 0.9771225745006943 22-23 0.9509918086251536 24-25 0.9432686266741896 26-27 0.9432286101873972 28-29 0.9660380076591556 30-31 0.9723606125723798 32-33 0.966278106579911 34-35 0.9772426239610719 36-37 0.9865264488969455 38-39 0.9715602828365286 40-41 0.982324717783727 42-43 0.9736411401497417 44-45 0.9579146608402662 46-47 0.9526324845836485 48-49 0.9576745619195107 50-51 0.983125047519578 52-53 0.9596753862591388 54-55 0.9741213379912523 56-57 1.0303845184215896 58-59 0.9898078008139353 60-61 1.0034134063234053 62-63 0.9711201014818104 64-65 0.9582748092213992 66-67 0.9612360292440485 68-69 0.9773626734214497 70-71 0.9714002168893584 72-73 0.9771225745006943 74-75 0.981604421021461 76-77 0.9952500430177234 78-79 1.0716415163047175 80-81 1.1099372941651962 82-83 1.0585161086367585 84-85 1.012417115851731 86-87 0.9968907189762183 88-89 0.9940495484139465 90-91 1.0060945109385067 92-93 0.9944497132818721 94-95 0.9889674545912917 96-97 0.9968507024894256 98-99 1.0317450789725366 100-101 1.0178193415687262 102-103 0.9935293340856433 104-105 0.9835652288742962 106-107 0.9856060697007167 108-109 0.983405162927126 110-111 0.9808441077724023 112-113 0.9917686086667707 114-115 0.9955701749120638 116-117 1.022381221063078 118-119 1.0170590283196677 120-121 0.9950499605837606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1249485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.20583154011814 #Duplication Level Percentage of deduplicated Percentage of total 1 83.19181677081573 50.08632506020108 2 11.137619733994908 13.410993149255857 3 2.542141064502223 4.591551500419124 4 0.9564144786528277 2.303269159372083 5 0.5283717365783225 1.5905529881497078 6 0.31693401471237026 1.1448765539463774 7 0.21091257754938833 0.8888716979541404 8 0.16587662729998912 0.7989392223732886 9 0.12419907520336709 0.6729757739219144 >10 0.7455999946650519 8.147473517468981 >50 0.050988513469859026 2.095016397123286 >100 0.021268330556984374 2.3793219672049797 >500 0.0032512063443942214 1.3334895237831899 >1k 0.003793074068459925 4.871192372934238 >5k 4.064007930492777E-4 1.550354884256554 >10k+ 4.064007930492777E-4 4.134796231635252 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 20305 1.625069528645802 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19100 1.528629795475736 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11292 0.903732337723142 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8369 0.6697959559338448 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5347 0.42793630975962094 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5293 0.4236145291860246 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3839 0.3072465855932644 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3819 0.3056459261215621 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3347 0.2678703625893868 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3254 0.2604272960459709 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3048 0.24394050348743682 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2791 0.22337202927606173 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2770 0.2216913368307743 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2481 0.19856180746467542 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2439 0.19520042257410053 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2316 0.18535636682313114 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2240 0.17927386083066224 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2223 0.17791330027971525 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2128 0.1703101677891291 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2087 0.16702881587213933 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2039 0.1631872331400537 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1846 0.1477408692381261 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1743 0.13949747295885906 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1696 0.13573592320035854 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1696 0.13573592320035854 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1652 0.1322144723626134 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1602 0.12821282368335754 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1353 0.1082846132606634 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1341 0.107324217577642 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1311 0.10492322837008848 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1279 0.10236217321536473 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 8.003297358511706E-5 0.0 6 0.0 0.0 0.0 8.003297358511706E-5 0.0 7 0.0 0.0 0.0 8.003297358511706E-5 0.0 8 0.0 0.0 0.0 8.003297358511706E-5 0.0 9 0.0 0.0 0.0 1.6006594717023412E-4 0.0 10-11 0.0 0.0 0.0 2.400989207553512E-4 0.0 12-13 0.0 0.0 0.0 3.601483811330268E-4 0.0 14-15 0.0 0.0 0.0 4.0016486792558535E-4 0.0 16-17 0.0 0.0 0.0 6.402637886809366E-4 0.0 18-19 0.0 0.0 0.0 0.0012004946037767559 0.0 20-21 0.0 0.0 0.0 0.0017607254188725754 0.0 22-23 0.0 0.0 0.0 0.002601071641516305 0.0 24-25 0.0 0.0 0.0 0.004521863007559114 0.0 26-27 0.0 0.0 0.0 0.007323017083038211 0.0 28-29 0.0 0.0 0.0 0.014525984705698748 0.0 30-31 0.0 0.0 0.0 0.029732249686870993 0.0 32-33 0.0 0.0 0.0 0.05370212527561355 0.0 34-35 0.0 0.0 0.0 0.07463074786812166 0.0 36-37 0.0 0.0 0.0 0.09679988155119909 0.0 38-39 0.0 0.0 0.0 0.11892899874748396 0.0 40-41 0.0 0.0 0.0 0.16498797504571883 0.0 42-43 0.0 0.0 0.0 0.2199306114119017 0.0 44-45 0.0 0.0 0.0 0.33925977502731125 0.0 46-47 0.0 0.0 0.0 0.5604308975297823 0.0 48-49 0.0 0.0 0.0 0.6905244960923901 0.0 50-51 0.0 0.0 0.0 0.8291816228286053 0.0 52-53 0.0 0.0 0.0 0.9742814039384227 0.0 54-55 0.0 0.0 0.0 1.1344674005690345 0.0 56-57 0.0 0.0 0.0 1.304737551871371 0.0 58-59 0.0 0.0 0.0 1.6238290175552328 0.0 60-61 0.0 0.0 0.0 1.8945005342200987 0.0 62-63 0.0 0.0 0.0 2.1893019924208774 0.0 64-65 0.0 0.0 0.0 2.3634137264553 0.0 66-67 0.0 0.0 0.0 2.8524552115471575 0.0 68-69 0.0 0.0 0.0 3.378872095303265 0.0 70-71 0.0 0.0 0.0 3.5743926497717062 0.0 72-73 0.0 0.0 0.0 4.097608214584408 0.0 74-75 0.0 0.0 0.0 4.352473218966214 0.0 76-77 0.0 0.0 0.0 4.517461194011933 0.0 78-79 0.0 0.0 0.0 4.733390156744578 0.0 80-81 0.0 0.0 0.0 4.932232079616802 0.0 82-83 0.0 0.0 0.0 5.289699356134728 0.0 84-85 0.0 0.0 0.0 5.993349259895076 0.0 86-87 0.0 0.0 0.0 7.302648691260799 0.0 88-89 0.0 0.0 0.0 8.777576361460923 0.0 90-91 0.0 0.0 0.0 10.270111285849769 0.0 92-93 0.0 0.0 0.0 11.689295989947858 0.0 94-95 0.0 0.0 0.0 12.89427244024538 0.0 96-97 0.0 0.0 0.0 14.07087720140698 0.0 98-99 0.0 0.0 0.0 15.287058268006419 0.0 100-101 0.0 8.003297358511706E-5 0.0 16.274705178533555 0.0 102-103 0.0 1.200494603776756E-4 0.0 17.09584348751686 0.0 104-105 0.0 1.6006594717023412E-4 0.0 18.06092109949299 0.0 106-107 0.0 1.6006594717023412E-4 0.0 19.22892231599419 0.0 108-109 0.0 1.6006594717023412E-4 0.0 20.46251055434839 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1040 0.0 36.495815 2 ACCTGGG 1105 0.0 32.751904 3 TATAACG 155 2.656052E-7 29.681835 2 TAGCGTG 105 7.2904426E-4 27.33084 9 GGGTACG 85 0.0083898865 27.144127 1 GAGTACG 450 0.0 25.636122 1 TATACCG 230 1.2032615E-8 24.976433 5 AATACCG 115 0.0012333697 24.976433 5 TACGCAG 420 0.0 23.251873 4 TACCGAC 125 0.0020097261 22.967178 7 GAGTACT 3555 0.0 22.869827 12-13 AGTACGC 430 0.0 22.735943 2 GTACATG 4705 0.0 22.067225 1 ATACCGA 185 3.5290643E-5 21.736248 6 CCGACCG 190 4.36459E-5 21.145437 9 TACATGG 4445 0.0 20.959248 2 TAGTACG 195 5.2950487E-5 20.621569 4 AGTACTT 3825 0.0 20.354193 12-13 GTACCTG 1930 0.0 20.024067 1 ACATGGG 4520 0.0 19.826427 3 >>END_MODULE