##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003813_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1249485 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.699014393930298 31.0 31.0 34.0 30.0 34.0 2 31.91314421541675 31.0 31.0 34.0 30.0 34.0 3 31.951813747263873 31.0 31.0 34.0 30.0 34.0 4 35.66918930599407 37.0 35.0 37.0 33.0 37.0 5 35.56006674749997 37.0 35.0 37.0 33.0 37.0 6 35.61374486288351 37.0 35.0 37.0 33.0 37.0 7 35.54880130613813 37.0 35.0 37.0 33.0 37.0 8 35.55233316126244 37.0 35.0 37.0 33.0 37.0 9 37.1229698635838 39.0 37.0 39.0 33.0 39.0 10-11 37.03807008487497 39.0 37.0 39.0 33.0 39.0 12-13 37.00427696210839 39.0 37.0 39.0 33.0 39.0 14-15 38.063380912936125 40.0 37.0 41.0 33.0 41.0 16-17 38.10308487096684 40.0 37.0 41.0 33.0 41.0 18-19 38.15162166812726 40.0 37.0 41.0 33.0 41.0 20-21 38.0925505308187 40.0 37.0 41.0 33.0 41.0 22-23 38.001826352457215 40.0 37.0 41.0 33.0 41.0 24-25 37.875419472822806 40.0 37.0 41.0 32.5 41.0 26-27 37.60930023169546 39.5 36.5 41.0 32.0 41.0 28-29 37.45524235985226 39.0 36.0 41.0 32.0 41.0 30-31 37.25810273832819 39.0 36.0 40.5 31.5 41.0 32-33 36.98585617274317 39.0 36.0 40.0 31.0 41.0 34-35 36.898308903268145 39.0 35.0 40.0 31.0 41.0 36-37 36.68137832787109 39.0 35.0 40.0 30.5 41.0 38-39 36.433524612140204 38.5 35.0 40.0 30.0 41.0 40-41 36.59251691696979 39.0 35.0 40.5 30.0 41.0 42-43 36.45885904992857 39.0 35.0 41.0 30.0 41.0 44-45 36.29911803663109 39.0 35.0 41.0 30.0 41.0 46-47 36.016609643172984 39.0 35.0 41.0 29.5 41.0 48-49 35.734564640631945 39.0 35.0 40.5 28.0 41.0 50-51 35.444989335606266 39.0 35.0 40.0 27.0 41.0 52-53 35.304259354854196 38.5 34.5 40.0 27.0 41.0 54-55 35.118892583744504 38.0 34.0 40.0 26.0 41.0 56-57 34.82573500282116 38.0 34.0 40.0 25.5 41.0 58-59 34.56415683261504 38.0 34.0 40.0 25.0 41.0 60-61 34.05705790785804 38.0 33.0 40.0 23.0 41.0 62-63 33.88455403626294 37.0 33.0 40.0 23.0 41.0 64-65 33.45598306502279 36.5 32.5 39.5 22.0 41.0 66-67 33.07384682489186 36.0 32.0 39.0 22.0 41.0 68-69 32.7742541927274 36.0 32.0 39.0 21.5 40.0 70-71 32.29113954949439 35.0 31.0 38.5 20.5 40.0 72-73 31.892784627266433 35.0 31.0 37.5 19.0 40.0 74-75 31.521399616642057 35.0 31.0 37.0 18.0 39.5 76-77 30.54238346198634 34.0 29.5 36.0 18.0 39.0 78-79 30.91076483511207 34.0 31.0 36.0 17.5 39.0 80-81 30.662853095475334 34.0 30.5 36.0 13.0 38.5 82-83 30.32920363189634 34.0 30.0 35.5 9.5 37.0 84-85 29.978241835636283 34.0 30.0 35.0 7.0 37.0 86-87 29.680629219238327 34.0 30.0 35.0 4.5 36.5 88-89 29.34091685774539 34.0 29.5 35.0 2.0 36.0 90-91 29.028897905937246 34.0 29.0 35.0 2.0 36.0 92-93 28.759021917029816 34.0 29.0 35.0 2.0 35.5 94-95 28.412907717979806 34.0 29.0 35.0 2.0 35.0 96-97 27.984300331736677 34.0 29.0 35.0 2.0 35.0 98-99 27.656861026743016 34.0 29.0 35.0 2.0 35.0 100-101 26.65226953504844 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.07008268811719276 1101 2 0.1399462743733757 1101 3 0.1215909583382988 1101 4 0.08075995787986301 1101 5 0.18655693916760185 1101 6 0.09616861975525381 1101 7 0.171843166068129 1101 8 0.19029400516927097 1101 9 0.24718069893325634 1101 10-11 0.23074774512223684 1101 12-13 0.20637838882533543 1101 14-15 0.06489849423902427 1101 16-17 0.12834999521930257 1101 18-19 0.027089132297824392 1101 20-21 0.032482120254648805 1101 22-23 0.14724821095848029 1101 24-25 0.2090584834471727 1101 26-27 0.2403514335689536 1101 28-29 0.20997507407040672 1101 30-31 0.26874197628983154 1101 32-33 0.3235974909866215 1101 34-35 0.2932030379162498 1101 36-37 0.24743941872492314 1101 38-39 0.29401291306083266 1101 40-41 0.4814845902884386 1101 42-43 0.3239667733941545 1101 44-45 0.20731693773081616 1101 46-47 0.11797878779290727 1101 48-49 0.1587301600784059 1101 50-51 0.1548118310006643 1101 52-53 0.32109299635224886 1101 54-55 0.3858237643608362 1101 56-57 0.3740906684740182 1101 58-59 0.3688630415750609 1101 60-61 0.3562120214335991 1101 62-63 0.08070169694292417 1101 64-65 0.0030375793519965555 1101 66-67 0.26463185671702405 1101 68-69 -0.0640103399401113 1101 70-71 0.3533039481824787 1101 72-73 0.09580461108676985 1101 74-75 0.171621970037247 1101 76-77 0.08540289109993893 1101 78-79 0.1659038077077426 1101 80-81 -0.19309413891993543 1101 82-83 -0.05695544536321506 1101 84-85 0.110933112861062 1101 86-87 0.16694851081188133 1101 88-89 -0.05529704390265877 1101 90-91 0.19474423139688568 1101 92-93 0.20943698555759482 1101 94-95 0.10932926472325732 1101 96-97 -0.04038215286083968 1101 98-99 -0.3309666596036678 1101 100-101 -0.4267306801439972 1102 1 -0.005091598590830415 1102 2 -0.0913745735212963 1102 3 -0.019960048706852973 1102 4 0.09736899821050571 1102 5 0.04747101413938992 1102 6 0.11197774647381209 1102 7 0.04417131360438731 1102 8 0.012292248523216642 1102 9 -0.09011253921746487 1102 10-11 -0.06764272964678497 1102 12-13 -0.07855696123488798 1102 14-15 0.07314317563886874 1102 16-17 0.005288822805937343 1102 18-19 -0.04755869593328299 1102 20-21 -0.010509281533771286 1102 22-23 -0.1623544665167671 1102 24-25 0.02156918736427116 1102 26-27 -0.12084773529122828 1102 28-29 -0.0539878203925781 1102 30-31 -0.09682232734169816 1102 32-33 -0.0612452121573952 1102 34-35 -0.26652980606895227 1102 36-37 -0.3507989830435534 1102 38-39 -0.38098933949039093 1102 40-41 -0.3067522089016066 1102 42-43 -0.20312024111227345 1102 44-45 -0.08788763862791171 1102 46-47 -0.0874903378114098 1102 48-49 -0.06637880493792636 1102 50-51 -0.0013783517276735324 1102 52-53 -0.24249532119488748 1102 54-55 -0.2225562764533322 1102 56-57 -0.1695630865460771 1102 58-59 -0.25054624663938796 1102 60-61 -0.05314944391459875 1102 62-63 -0.12173800518382905 1102 64-65 -0.3606841029839387 1102 66-67 -0.35481766790869784 1102 68-69 -0.4584399698958066 1102 70-71 -0.3250066315175957 1102 72-73 -0.3195985061690152 1102 74-75 -0.29452098133446825 1102 76-77 -0.2693759909055835 1102 78-79 -0.32593920563492773 1102 80-81 -0.34829595031502336 1102 82-83 -0.555955037496684 1102 84-85 -0.47990144533282475 1102 86-87 -0.5694926783425878 1102 88-89 -0.6234853805706848 1102 90-91 -0.41178115158841067 1102 92-93 -0.2109114273739081 1102 94-95 -0.3606010678220848 1102 96-97 -0.17393127257757257 1102 98-99 -0.2735410470988775 1102 100-101 -0.2356080705135355 1103 1 -0.1666679736186758 1103 2 -0.2087175607797107 1103 3 -0.20075428467628242 1103 4 -0.1585824607212203 1103 5 -0.12201556050224127 1103 6 -0.14972859658573157 1103 7 -0.181843978661405 1103 8 -0.18989065382078252 1103 9 -0.32364193102313266 1103 10-11 -0.23927392433476058 1103 12-13 -0.27948960356905417 1103 14-15 -0.32377786546259557 1103 16-17 -0.29451504496007885 1103 18-19 -0.4010274851652653 1103 20-21 -0.12382303852453447 1103 22-23 -0.24851545279857135 1103 24-25 -0.3430582646574649 1103 26-27 -0.3112796687759172 1103 28-29 -0.19949743227351746 1103 30-31 -0.4057191628192811 1103 32-33 -0.11324939001739409 1103 34-35 -0.019853874938732474 1103 36-37 -0.06888637289896593 1103 38-39 -0.0363667852423859 1103 40-41 0.12697774083720503 1103 42-43 0.19479068136570277 1103 44-45 0.06724860652041542 1103 46-47 0.23280525463480473 1103 48-49 0.09408708841157676 1103 50-51 0.35465328612031755 1103 52-53 0.3218864307623832 1103 54-55 0.07352336547810268 1103 56-57 0.21708295636255315 1103 58-59 0.26013554706697306 1103 60-61 0.3564530692924279 1103 62-63 0.4055774099688776 1103 64-65 0.16845285617718275 1103 66-67 -0.05849830060664374 1103 68-69 0.2703919110705826 1103 70-71 0.1519203547730612 1103 72-73 0.2970430077741497 1103 74-75 0.12067709083098421 1103 76-77 0.10393837093073088 1103 78-79 0.2695426718646736 1103 80-81 0.17662073589848148 1103 82-83 0.24554719231806743 1103 84-85 0.07121169176500786 1103 86-87 0.16893227433931557 1103 88-89 0.20512019964585093 1103 90-91 0.17912049609938308 1103 92-93 0.18098091306857356 1103 94-95 -0.17511927883646905 1103 96-97 0.056209877720643675 1103 98-99 -0.13320765918192023 1103 100-101 -0.1342267360472249 1104 1 -0.07031211160873241 1104 2 -0.028575255097567975 1104 3 -0.06070880554075231 1104 4 -0.08680611537320715 1104 5 -0.025977563418038585 1104 6 -0.05097805025538804 1104 7 0.03038434615987029 1104 8 -0.06433400815154044 1104 9 -0.0941266444709612 1104 10-11 -0.08728023884376768 1104 12-13 0.02242706080532031 1104 14-15 -0.04237800666798108 1104 16-17 -0.07807806626618685 1104 18-19 0.005239038462185874 1104 20-21 -0.14153025436398536 1104 22-23 -0.06692823708137752 1104 24-25 0.10621477214456831 1104 26-27 0.06145134108706429 1104 28-29 -0.06297903125634718 1104 30-31 -0.2736653886317484 1104 32-33 0.03946789663869765 1104 34-35 0.016823611360983648 1104 36-37 -0.05765641901994201 1104 38-39 0.1347117854657469 1104 40-41 0.012453911665154749 1104 42-43 0.02346759917622876 1104 44-45 0.2504763414577411 1104 46-47 -0.08917281268531951 1104 48-49 -0.17950062855415894 1104 50-51 8.818493402955596E-4 1104 52-53 -0.18574232745549324 1104 54-55 -0.32795226131179334 1104 56-57 -0.3854727998961849 1104 58-59 -0.22459853141510422 1104 60-61 -0.004832510948595825 1104 62-63 -0.1610137637779232 1104 64-65 -0.2153835595290161 1104 66-67 -0.3481825140284869 1104 68-69 -0.5091122196452531 1104 70-71 -0.3963515603325476 1104 72-73 -0.36374774235650875 1104 74-75 -0.47500101591661803 1104 76-77 -0.7286094692687932 1104 78-79 -0.6228149689662992 1104 80-81 -0.6941511257202428 1104 82-83 -0.6529071894804979 1104 84-85 -0.6438598431252522 1104 86-87 -0.7404082170676354 1104 88-89 -0.5949588081265595 1104 90-91 -0.6798597993316129 1104 92-93 -0.8089742496385313 1104 94-95 -0.6075233157210285 1104 96-97 -0.3042405429919235 1104 98-99 -0.5086145415688463 1104 100-101 -0.48288635467044116 1105 1 0.05422344069070206 1105 2 0.09613511070763892 1105 3 0.022704253345970926 1105 4 0.1305776574836628 1105 5 0.015397614971199403 1105 6 0.08575630631966646 1105 7 -0.057513473756792166 1105 8 0.11432221632171746 1105 9 0.03767479281390251 1105 10-11 0.21475935800450685 1105 12-13 -0.005735394974813346 1105 14-15 0.194774789871353 1105 16-17 0.10691991543242096 1105 18-19 0.12712139850808768 1105 20-21 0.047285813222089246 1105 22-23 0.05329816230074158 1105 24-25 0.14311949242706845 1105 26-27 0.11453425672207374 1105 28-29 0.04364608276973314 1105 30-31 -0.11591924326955194 1105 32-33 0.2787057415377987 1105 34-35 0.18363304239070288 1105 36-37 0.11357340988531206 1105 38-39 0.1261459236828415 1105 40-41 0.05742389141857274 1105 42-43 0.18330197132556947 1105 44-45 0.09874298608730925 1105 46-47 -0.1258049828201777 1105 48-49 -0.17887924600182004 1105 50-51 -0.21937283804548713 1105 52-53 -0.19520291654522026 1105 54-55 -0.11689117511602376 1105 56-57 -0.04862068258677965 1105 58-59 -0.04361172506714439 1105 60-61 -0.13580596790156818 1105 62-63 -0.19970237642615274 1105 64-65 -0.3081833090689372 1105 66-67 -0.3652252416277335 1105 68-69 -0.4252804029515147 1105 70-71 -0.2572373562584396 1105 72-73 -0.4396660694866128 1105 74-75 -0.5266989318217874 1105 76-77 -0.4967567003817841 1105 78-79 -0.45531820301615866 1105 80-81 -0.3443739334794209 1105 82-83 -0.2983583831389005 1105 84-85 -0.4494131914395716 1105 86-87 -0.4468891894121363 1105 88-89 -0.3979582900428973 1105 90-91 -0.3499271184234729 1105 92-93 -0.49807240265438324 1105 94-95 -0.47217872651687287 1105 96-97 -0.2523002719992071 1105 98-99 -0.47064981575588405 1105 100-101 -0.1749364721133304 1106 1 -0.11526842588695274 1106 2 -0.08158243192196224 1106 3 -0.08253709912693097 1106 4 -0.06662554556507416 1106 5 -0.08522602009326619 1106 6 -0.019996634896273235 1106 7 -0.07755936578305267 1106 8 -0.05664570958010273 1106 9 -0.10949838584630811 1106 10-11 -0.1817613353408163 1106 12-13 -0.10470097930979705 1106 14-15 -0.11653366962592315 1106 16-17 -0.010562665390210668 1106 18-19 -0.013964712780079935 1106 20-21 -0.16915228789468273 1106 22-23 -0.23237718516026717 1106 24-25 -0.2246507522297705 1106 26-27 0.023323854529444077 1106 28-29 0.07126432686298045 1106 30-31 0.023473593146171368 1106 32-33 -0.13686282644982128 1106 34-35 -0.15278268300858855 1106 36-37 -0.05576833474972176 1106 38-39 -0.01885509949465103 1106 40-41 -0.1281788272143345 1106 42-43 -0.03941944595975855 1106 44-45 -0.13752295283737936 1106 46-47 -0.2729093905346005 1106 48-49 -0.2242999396826022 1106 50-51 -0.21245548730359332 1106 52-53 -0.2949274972940614 1106 54-55 -0.247421784896261 1106 56-57 -0.2514002029345974 1106 58-59 -0.18446804075598067 1106 60-61 -0.014998060249205025 1106 62-63 -0.018871690494677296 1106 64-65 -0.06579759463944868 1106 66-67 -0.17597514685801485 1106 68-69 -0.18764827048190114 1106 70-71 -0.06436840013430611 1106 72-73 -0.1571793456338284 1106 74-75 7.784156996102354E-4 1106 76-77 -0.03359779924085515 1106 78-79 -0.0194211111884961 1106 80-81 -0.0786874484798723 1106 82-83 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-0.9317836369018089 2316 82-83 -1.0074870469836377 2316 84-85 -0.9759789058669206 2316 86-87 -0.8431510556836983 2316 88-89 -0.8539239918018708 2316 90-91 -1.0768837622962515 2316 92-93 -1.3389445650078358 2316 94-95 -1.330936702771936 2316 96-97 -1.0755971332722645 2316 98-99 -1.166796151979991 2316 100-101 -1.3984306570550444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 13.0 5 26.0 6 71.0 7 180.0 8 450.0 9 851.0 10 1477.0 11 2649.0 12 4245.0 13 5933.0 14 6321.0 15 7795.0 16 9670.0 17 12160.0 18 10631.0 19 8084.0 20 7213.0 21 7355.0 22 7846.0 23 8504.0 24 9857.0 25 11519.0 26 14348.0 27 18034.0 28 22484.0 29 27956.0 30 34369.0 31 42605.0 32 52478.0 33 66258.0 34 86279.0 35 115272.0 36 162845.0 37 221608.0 38 211789.0 39 50280.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.55928164426209 20.50323016658499 14.069961852965765 19.867526336187154 2 14.746856921495871 24.31599824557472 38.32514814968961 22.611996683239795 3 22.891523816844746 26.652701975555388 29.843681938145387 20.61209226945448 4 12.325077932108028 17.628302860778643 40.991768608666774 29.054850598446556 5 11.596537773562709 40.48467968803146 34.58280811694418 13.335974421461641 6 27.01985641912171 39.925568441018996 17.719433039608795 15.335142100250506 7 26.287630503767552 33.82945773658748 23.18251119461218 16.700400565032794 8 23.729296470145698 33.286994241627546 20.34894376483111 22.63476552339564 9 23.284233104038865 20.99304913624413 23.617730504968044 32.104987254748956 10-11 24.345678063803316 26.349230740295663 28.671795998416943 20.633295197484074 12-13 28.040192559334447 24.254152710916898 27.604853199518203 20.100801530230456 14-15 22.446996678538557 27.288126775781347 28.905598463323862 21.359278082356237 16-17 19.68735118868974 29.792354450033415 29.683309523523693 20.836984837753153 18-19 19.22920243140174 31.74263796684234 29.851338751565642 19.17682085019028 20-21 21.520680909784737 27.976188808627995 29.074378756267865 21.4287515253194 22-23 19.986634493411284 28.68225708992105 29.246569586669708 22.08453882999796 24-25 22.32339723966274 28.6943420689324 29.14200650668075 19.840254184724106 26-27 22.100264490023296 26.417626905386687 32.03042615749902 19.451682447090988 28-29 19.75651338696122 30.70528674910954 30.321207027654378 19.216992836274862 30-31 21.215328943313676 28.293095043898862 29.461943780320794 21.029632232466668 32-33 21.22573495954676 28.802858639568978 29.979458230334348 19.991948170549914 34-35 22.71067914851111 27.106991583079076 30.437742318639206 19.744586949770607 36-37 22.486197309826267 26.853106715988385 31.39216450677346 19.268531467411883 38-39 21.108016589552648 28.62838433557545 31.35626356465671 18.90733551021519 40-41 20.5961915365968 29.21940468095444 29.97719116318664 20.207212619262123 42-43 19.877589566901563 29.965545804871606 28.41106535892788 21.745799269298953 44-45 21.469525444483125 27.777564356514883 28.456283989003467 22.296626209998518 46-47 22.76294743952406 29.36851157417319 28.29935065220897 19.56919033409378 48-49 22.510834463799085 28.70634701497017 28.765771497857116 20.017047023373628 50-51 19.98867533423771 29.218037831586614 30.797208449881353 19.99607838429433 52-53 21.142190582519998 30.507409052529646 28.18229110393482 20.168109261015537 54-55 20.108284613260665 29.490590123130726 28.41078524352033 21.990340020088276 56-57 22.035494235358097 27.994418435649344 28.806962629369803 21.16312469962276 58-59 24.085581064793253 27.544814571158287 27.783073305577833 20.58653105847063 60-61 21.581148521825437 29.014123400661944 30.23612372883391 19.16860434867871 62-63 20.580647381927804 28.65599170080639 30.12087781902827 20.64248309823754 64-65 20.111766957206843 31.45176141649946 28.321421880029273 20.115049746264425 66-67 21.144717102872118 28.2925546477615 27.89425399981748 22.668474249548908 68-69 20.435539107600626 29.643334776620033 27.69485771087259 22.226268404906747 70-71 22.33251245917305 29.29428298629591 27.31282028298149 21.060384271549548 72-73 21.78365486580471 27.237861999143647 29.886193111561965 21.092290023489678 74-75 20.38367807536705 31.1766047611616 28.26152374778409 20.17819341568726 76-77 21.363161622588507 28.64616221883416 27.738068084050628 22.252608074526705 78-79 21.2948934961204 29.16449577225817 28.085851370764754 21.454759360856674 80-81 22.358491698579815 28.807868841962893 27.904856801002015 20.928782658455283 82-83 21.5670056063098 28.079448732877943 29.33184472002465 21.021700940787603 84-85 20.589675889948083 29.223886999663062 28.897792126823653 21.288644983565202 86-87 20.828267872506455 29.161327757658217 27.437323675943897 22.573080693891434 88-89 20.422414034582246 29.319199510198203 27.083958590939467 23.174427864280084 90-91 21.39405435039236 26.38943244616782 28.034310135775943 24.182203067663878 92-93 21.75236197313293 28.227629783470793 28.13955349604037 21.88045474735591 94-95 20.79372187080951 28.298240748855225 28.933904930889277 21.97413244944599 96-97 18.751900796814024 28.564494235983723 30.45284583297318 22.230759134229068 98-99 20.044570791045505 29.9436242319762 27.76934755693 22.242457420048293 100-101 19.02304664552697 28.995106709586338 27.61823851149211 24.36360813339459 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 214.0 1 165.0 2 283.5 3 394.0 4 279.5 5 246.0 6 337.0 7 540.0 8 797.0 9 978.5 10 1024.0 11 1028.0 12 1083.0 13 1222.0 14 1370.0 15 1466.5 16 1601.0 17 1777.0 18 1978.5 19 2234.5 20 2461.0 21 2739.0 22 3223.0 23 3876.0 24 4559.5 25 5513.5 26 6910.0 27 8802.5 28 11683.0 29 15544.5 30 19587.0 31 23401.5 32 27188.0 33 30656.5 34 33355.0 35 35098.0 36 36479.5 37 38214.0 38 39610.5 39 39894.5 40 40160.5 41 40410.0 42 40829.5 43 42234.5 44 42653.0 45 43119.5 46 46224.5 47 63287.0 48 104800.0 49 103356.5 50 62941.0 51 44005.0 52 33002.5 53 26300.5 54 22315.0 55 19096.5 56 15749.5 57 12671.0 58 10040.0 59 7904.5 60 6175.5 61 4663.0 62 3503.0 63 2404.5 64 1551.5 65 983.0 66 582.0 67 344.5 68 187.0 69 98.5 70 59.0 71 32.5 72 23.0 73 20.5 74 15.0 75 12.0 76 8.0 77 3.0 78 2.5 79 2.0 80 2.5 81 2.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050660872279379106 2 0.005842407071713546 3 0.04970047659635769 4 0.0 5 0.0 6 0.001200494603776756 7 0.0 8 0.0 9 0.0 10-11 3.601483811330268E-4 12-13 0.0 14-15 0.0028011540754790975 16-17 0.0 18-19 0.0 20-21 0.012525160366070822 22-23 0.0 24-25 0.0 26-27 0.007963280871719148 28-29 0.010804451433990803 30-31 0.01088448440757592 32-33 0.006362621400016807 34-35 0.007763198437756355 36-37 0.02176896881515184 38-39 0.011564764683049417 40-41 0.015086215520794567 42-43 0.0 44-45 0.0 46-47 4.001648679255853E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011604781169841974 58-59 2.4009892075535123E-4 60-61 0.01120461630191639 62-63 0.016566825532119234 64-65 0.04377803655105904 66-67 0.024249990996290473 68-69 0.0017607254188725754 70-71 3.2013189434046825E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.6006594717023412E-4 86-87 8.003297358511706E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 3.601483811330268E-4 96-97 7.202967622660536E-4 98-99 9.603956830214049E-4 100-101 0.0015206264981172243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1249485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.73561986889095 #Duplication Level Percentage of deduplicated Percentage of total 1 81.45891347994578 46.21621950131081 2 12.187241874795998 13.829014445173113 3 2.8129331805155506 4.787805229389621 4 1.1020020768289243 2.5009108370277686 5 0.5766952878354891 1.635958231540748 6 0.358987222806663 1.2220417566568618 7 0.24353830304322233 0.9672107609482521 8 0.19025627934611083 0.8635446354120379 9 0.137756232787618 0.7034116732207871 >10 0.8345478367013345 8.836284389244756 >50 0.059460953622582746 2.3310740115425768 >100 0.029849543215847665 3.1628717008140317 >500 0.0027006698643830067 1.0324890323126823 >1k 0.0038377940178074303 4.759949437102535 >5k 9.94983634246371E-4 3.5752039576285357 >10k+ 2.8428103835610594E-4 3.5760104006749414 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 33746 2.7007927266033604 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10597 0.8481094210814855 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8071 0.6459461298054798 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7184 0.574956882235481 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6297 0.5039676346654822 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6174 0.4941235789145128 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5828 0.4664321700540623 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5702 0.4563480153823375 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5077 0.4063274068916394 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4351 0.3482234680688444 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4237 0.339099709080141 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3863 0.30916737695930724 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3660 0.2929206833215285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3596 0.28779857301208095 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3326 0.2661896701440994 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3320 0.2657094723025887 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3169 0.253624493291236 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2586 0.20696526969111273 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2378 0.1903184111854084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1974 0.1579850898570211 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1810 0.1448596821890619 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1762 0.14101809945697627 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1743 0.13949747295885906 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1709 0.13677635185696507 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1481 0.11852883387955838 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1476 0.1181286690116328 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1412 0.1130065587021853 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1411 0.1129265257286002 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1409 0.11276645978142996 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1298 0.10388279971348195 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 8.003297358511706E-5 0.0 6 0.0 0.0 0.0 8.003297358511706E-5 0.0 7 0.0 0.0 0.0 8.003297358511706E-5 0.0 8 0.0 0.0 0.0 8.003297358511706E-5 0.0 9 0.0 0.0 0.0 1.6006594717023412E-4 0.0 10-11 0.0 0.0 0.0 2.400989207553512E-4 0.0 12-13 0.0 0.0 0.0 3.601483811330268E-4 0.0 14-15 0.0 0.0 0.0 4.0016486792558535E-4 8.003297358511706E-5 16-17 0.0 0.0 0.0 6.402637886809366E-4 8.003297358511706E-5 18-19 0.0 0.0 0.0 0.0011604781169841974 8.003297358511706E-5 20-21 0.0 0.0 0.0 0.001720708932080017 8.003297358511706E-5 22-23 0.0 0.0 0.0 0.002601071641516305 8.003297358511706E-5 24-25 0.0 0.0 0.0 0.004521863007559114 8.003297358511706E-5 26-27 0.0 0.0 0.0 0.007483083030208447 8.003297358511706E-5 28-29 0.0 0.0 0.0 0.014806100113246658 8.003297358511706E-5 30-31 0.0 0.0 0.0 0.030812694830270073 8.003297358511706E-5 32-33 0.0 0.0 0.0 0.056383229890714974 8.003297358511706E-5 34-35 0.0 0.0 0.0 0.07899254492851054 8.003297358511706E-5 36-37 0.0 0.0 0.0 0.1022021072681945 8.003297358511706E-5 38-39 0.0 0.0 0.0 0.12541166960787845 8.003297358511706E-5 40-41 0.0 0.0 0.0 0.17295125591743798 8.003297358511706E-5 42-43 0.0 0.0 0.0 0.2299747495968339 8.003297358511706E-5 44-45 0.0 0.0 0.0 0.3526252816160258 8.003297358511706E-5 46-47 0.0 0.0 0.0 0.5805191738996467 8.003297358511706E-5 48-49 0.0 0.0 0.0 0.7146944541150955 8.003297358511706E-5 50-51 0.0 0.0 0.0 0.8565528997947154 8.003297358511706E-5 52-53 0.0 0.0 0.0 1.0055342801234108 8.003297358511706E-5 54-55 0.0 0.0 0.0 1.1698019584068637 8.003297358511706E-5 56-57 0.0 0.0 0.0 1.3433134451393975 8.003297358511706E-5 58-59 0.0 0.0 0.0 1.6657262792270415 8.003297358511706E-5 60-61 0.0 0.0 0.0 1.9426403678315465 8.003297358511706E-5 62-63 0.0 0.0 0.0 2.245685222311592 8.003297358511706E-5 64-65 0.0 0.0 0.0 2.425879462338483 8.003297358511706E-5 66-67 0.0 0.0 0.0 2.9250851350756513 8.003297358511706E-5 68-69 0.0 0.0 0.0 3.4649475583940585 8.003297358511706E-5 70-71 0.0 0.0 0.0 3.6679912123795004 8.003297358511706E-5 72-73 0.0 0.0 0.0 4.209734410577157 8.003297358511706E-5 74-75 0.0 0.0 0.0 4.477124575325034 8.003297358511706E-5 76-77 0.0 0.0 0.0 4.651196292872664 8.003297358511706E-5 78-79 0.0 0.0 0.0 4.8775295421713745 8.003297358511706E-5 80-81 0.0 0.0 0.0 5.089576905685142 8.003297358511706E-5 82-83 0.0 0.0 0.0 5.463290875840846 8.003297358511706E-5 84-85 0.0 0.0 0.0 6.188389616522007 8.003297358511706E-5 86-87 0.0 0.0 0.0 7.545268650684082 8.003297358511706E-5 88-89 0.0 0.0 0.0 9.07017691288811 8.003297358511706E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1115 0.0 39.630287 2 ATAACGC 230 0.0 37.181686 3 TATAACG 210 0.0 36.20094 2 ACCTGGG 1275 0.0 35.026928 3 TCACGCA 150 2.5465852E-11 34.825275 4 TACGCAG 445 0.0 32.015068 4 GTACACG 135 1.186163E-8 31.697397 1 GTACGCA 470 0.0 30.325485 3 TAACGCA 300 0.0 30.076374 4 AGTACGC 475 0.0 30.008673 2 GTACCTG 1565 0.0 28.861849 1 GAGTACG 540 0.0 28.175465 1 CCGGTCG 135 4.2312058E-7 28.143892 9 ACGCAGT 540 0.0 26.383842 5 CGCAGTG 580 0.0 24.564268 6 GTATAAC 415 0.0 24.05947 1 GTACCCG 105 0.002183981 22.640997 1 TATCACG 210 3.436253E-8 22.625587 2 CCTGGGG 1025 0.0 22.238758 4 CACGCAG 235 4.660251E-9 22.22979 5 >>END_MODULE