##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003812_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1821807 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.74958489016674 31.0 31.0 34.0 28.0 34.0 2 30.645039787419854 31.0 31.0 34.0 27.0 34.0 3 30.710271724721665 31.0 31.0 34.0 27.0 34.0 4 34.188194468459066 37.0 35.0 37.0 30.0 37.0 5 34.14974363365604 37.0 35.0 37.0 30.0 37.0 6 34.26171762431476 37.0 35.0 37.0 30.0 37.0 7 34.30908048986528 37.0 35.0 37.0 30.0 37.0 8 34.27881164140878 37.0 35.0 37.0 30.0 37.0 9 35.74218289862757 39.0 35.0 39.0 30.0 39.0 10-11 35.57278405451291 39.0 35.0 39.0 30.0 39.0 12-13 35.41578032140617 39.0 35.0 39.0 30.0 39.0 14-15 36.39607461163559 39.0 36.0 41.0 29.0 41.0 16-17 36.474235745059715 39.0 36.0 41.0 30.0 41.0 18-19 36.46493701034193 39.0 36.0 41.0 29.5 41.0 20-21 36.337775626067966 39.0 36.0 41.0 29.0 41.0 22-23 36.25310063030826 39.0 36.0 41.0 29.0 41.0 24-25 36.06669614289549 39.0 36.0 41.0 27.0 41.0 26-27 35.86809552274198 39.0 35.0 41.0 27.0 41.0 28-29 35.67149538891881 39.0 35.0 40.0 27.0 41.0 30-31 35.4493272338947 38.0 35.0 40.0 26.5 41.0 32-33 35.194134724479596 38.0 34.0 40.0 25.5 41.0 34-35 35.15367956100729 38.0 34.5 40.0 25.0 41.0 36-37 35.00764076545979 38.0 34.0 40.0 25.0 41.0 38-39 35.02233661414189 38.0 34.5 40.0 25.0 41.0 40-41 35.086803377086596 38.0 35.0 40.0 25.0 41.0 42-43 34.93488196060285 38.0 34.5 40.0 24.0 41.0 44-45 34.70556787848548 38.0 34.0 40.0 23.5 41.0 46-47 34.41287441534696 38.0 34.0 40.0 22.5 41.0 48-49 34.14488719167288 38.0 33.5 40.0 19.5 41.0 50-51 33.399705621945685 37.5 32.5 39.5 18.5 40.5 52-53 33.536212123457645 38.0 33.0 39.5 19.0 40.5 54-55 33.856295699818915 38.0 33.0 40.0 19.0 41.0 56-57 33.67285365573851 38.0 33.0 40.0 16.5 41.0 58-59 33.40882815797722 38.0 33.0 40.0 14.0 41.0 60-61 33.147663281566054 37.5 32.5 40.0 13.0 41.0 62-63 32.88443095234567 37.0 32.0 40.0 12.5 41.0 64-65 32.59031060919186 36.5 32.0 40.0 9.0 41.0 66-67 32.2420086211108 36.0 31.0 39.0 8.0 41.0 68-69 31.872478533675633 36.0 31.0 39.0 7.0 41.0 70-71 31.476685785047483 35.0 31.0 39.0 6.0 40.0 72-73 31.122647184910367 35.0 31.0 38.0 2.0 40.0 74-75 30.755288567888915 35.0 30.0 37.0 2.0 39.5 76-77 30.328396751137745 35.0 30.0 37.0 2.0 39.0 78-79 29.779315536717117 34.0 29.0 36.0 2.0 39.0 80-81 29.378450626218914 34.0 29.0 36.0 2.0 38.5 82-83 29.04344340536621 34.0 29.0 35.5 2.0 37.0 84-85 28.80364934375595 34.0 29.0 35.0 2.0 37.0 86-87 28.552990794304776 34.0 29.0 35.0 2.0 36.5 88-89 28.21291223494036 34.0 27.5 35.0 2.0 36.0 90-91 27.86048851497442 34.0 27.0 35.0 2.0 36.0 92-93 27.593529665875693 34.0 26.0 35.0 2.0 35.0 94-95 27.356803711918992 34.0 26.0 35.0 2.0 35.0 96-97 27.06475027266884 34.0 25.0 35.0 2.0 35.0 98-99 26.774345471282086 33.0 25.0 35.0 2.0 35.0 100-101 26.460550705974892 33.0 25.0 35.0 2.0 35.0 102-103 26.141812497152554 33.0 24.0 35.0 2.0 35.0 104-105 25.816469856576465 33.0 22.5 35.0 2.0 35.0 106-107 25.475735629515093 32.0 19.5 35.0 2.0 35.0 108-109 25.10799140633448 32.0 18.5 35.0 2.0 35.0 110-111 24.681376237987887 32.0 16.5 34.5 2.0 35.0 112-113 24.19052814046713 32.0 7.0 34.0 2.0 35.0 114-115 23.662271854263377 31.0 2.0 34.0 2.0 35.0 116-117 22.996763652790882 31.0 2.0 34.0 2.0 35.0 118-119 22.221065403744745 31.0 2.0 34.0 2.0 35.0 120-121 20.58155035083299 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.45755522400070703 1101 2 0.46182850366322725 1101 3 0.6080781277132417 1101 4 0.6521148957676814 1101 5 0.7017142385433317 1101 6 0.8264992893263212 1101 7 0.5530986034392669 1101 8 0.6948729499710282 1101 9 0.8271468188857085 1101 10-11 0.7372746514771151 1101 12-13 0.8319741658876367 1101 14-15 1.0201234407926663 1101 16-17 0.8669856230951964 1101 18-19 0.7559007643742106 1101 20-21 0.8215000258667047 1101 22-23 0.8298491423632655 1101 24-25 0.7616669457167546 1101 26-27 0.6935636799453064 1101 28-29 0.7632178546944459 1101 30-31 0.8175903931315958 1101 32-33 0.6298090572688366 1101 34-35 0.7851106227131766 1101 36-37 0.7818902477583478 1101 38-39 0.7596703626152461 1101 40-41 0.6031984328957236 1101 42-43 0.4978378737846043 1101 44-45 0.6052313367906876 1101 46-47 0.6074407958412351 1101 48-49 0.7372095006313657 1101 50-51 0.5034368204436959 1101 52-53 0.6076704160952957 1101 54-55 0.7322179338284371 1101 56-57 0.6380087754436872 1101 58-59 0.5653905320863117 1101 60-61 0.607909731918383 1101 62-63 0.6131370320480514 1101 64-65 0.7468471597938589 1101 66-67 0.643441985676489 1101 68-69 0.43095306423020574 1101 70-71 0.20728232382907308 1101 72-73 0.22623603826755811 1101 74-75 0.2272509715237092 1101 76-77 0.007999288800359494 1101 78-79 0.30878460318106704 1101 80-81 0.45250498849974363 1101 82-83 0.4447143642134641 1101 84-85 0.15609129214522355 1101 86-87 0.18753126834846512 1101 88-89 0.2847878490443314 1101 90-91 0.3469977779513691 1101 92-93 0.36959818901368635 1101 94-95 0.4904217253637917 1101 96-97 0.2990935065252174 1101 98-99 0.2127210771128425 1101 100-101 0.3440210989689412 1101 102-103 0.1550050772884326 1101 104-105 0.19275722300911724 1101 106-107 0.17028431528595434 1101 108-109 0.13016751222690104 1101 110-111 0.25688938361675895 1101 112-113 0.3642310367956725 1101 114-115 0.3481095153948601 1101 116-117 0.20234368579650308 1101 118-119 0.46561830439597784 1101 120-121 0.59642382152051 1102 1 0.5269066596488123 1102 2 0.35010966513249286 1102 3 0.5202022177859256 1102 4 0.723775887473785 1102 5 0.7903035206922979 1102 6 0.7300192038511568 1102 7 0.553905868827762 1102 8 0.7324982083121441 1102 9 0.6652474788359513 1102 10-11 0.6632013068837992 1102 12-13 0.6928074947241711 1102 14-15 0.7590877559931002 1102 16-17 0.7443050271433975 1102 18-19 0.7072635643331964 1102 20-21 0.7051863579355384 1102 22-23 0.6658790262918828 1102 24-25 0.828421210031415 1102 26-27 0.9317852138574594 1102 28-29 0.9187432517168475 1102 30-31 0.8789584813264213 1102 32-33 0.647140170982091 1102 34-35 0.6868657234324829 1102 36-37 0.7892252467060956 1102 38-39 0.6899363392930482 1102 40-41 0.678907353286192 1102 42-43 0.5555673319562828 1102 44-45 0.8221105221865201 1102 46-47 0.7450738786129207 1102 48-49 0.5498610652476756 1102 50-51 0.2772978261798116 1102 52-53 0.3102074202557361 1102 54-55 0.35214918676259543 1102 56-57 0.5841170655627863 1102 58-59 0.5565489255418541 1102 60-61 0.5323386833907264 1102 62-63 0.4500119544782635 1102 64-65 0.37719569770457184 1102 66-67 0.11856434358212908 1102 68-69 0.26070127945061117 1102 70-71 0.1645452840539825 1102 72-73 0.05265401395246627 1102 74-75 0.19029105958902548 1102 76-77 0.15767012310050532 1102 78-79 0.39532361471265887 1102 80-81 0.596337448554678 1102 82-83 0.5162534027258516 1102 84-85 0.3290120171731452 1102 86-87 0.48613285373998494 1102 88-89 0.40032959642854493 1102 90-91 0.43393394678639297 1102 92-93 0.3773991462922943 1102 94-95 0.2791812563296503 1102 96-97 0.2892989382988844 1102 98-99 0.4026108335841876 1102 100-101 0.4723056060567963 1102 102-103 0.32268640172170393 1102 104-105 0.5078969565467837 1102 106-107 0.2516273653029515 1102 108-109 0.09947362013928895 1102 110-111 0.05294344233776371 1102 112-113 0.060253258178491365 1102 114-115 0.11976349209424697 1102 116-117 -0.039514319675198806 1102 118-119 0.08620761892190032 1102 120-121 0.16569728267086248 1103 1 0.7409249729993483 1103 2 0.7686735818207353 1103 3 0.923078967210845 1103 4 1.0683995769860175 1103 5 1.1554986319740195 1103 6 0.9954550609986939 1103 7 0.9464489962623759 1103 8 0.919565185657035 1103 9 0.9738393387093609 1103 10-11 1.0737075278879047 1103 12-13 1.011140783419826 1103 14-15 1.2616728726015864 1103 16-17 1.1955423835216763 1103 18-19 0.9879589598081253 1103 20-21 1.0397421684218173 1103 22-23 1.0986008234642313 1103 24-25 0.9714443412751095 1103 26-27 1.2372363668486344 1103 28-29 1.1549769924433733 1103 30-31 1.3167347513427003 1103 32-33 1.0985327176805342 1103 34-35 1.1163356659094035 1103 36-37 0.9683246964723438 1103 38-39 0.9257930404497685 1103 40-41 1.1740357461215467 1103 42-43 1.0432012077861685 1103 44-45 1.2220498149471766 1103 46-47 1.1917065611289317 1103 48-49 1.0547740366587206 1103 50-51 0.9516899354290373 1103 52-53 0.9164150330625844 1103 54-55 0.8374489585152745 1103 56-57 0.8755359187316074 1103 58-59 0.9611306853971016 1103 60-61 0.8773379245502326 1103 62-63 1.0079834994477537 1103 64-65 0.8871607612835035 1103 66-67 0.8723109325197314 1103 68-69 0.6815686490118757 1103 70-71 0.6486335892804611 1103 72-73 0.7096622778742159 1103 74-75 0.7713756955985325 1103 76-77 0.692493908434944 1103 78-79 0.8027709645738348 1103 80-81 0.9097394643921248 1103 82-83 0.7994272307636656 1103 84-85 0.7761794590474516 1103 86-87 0.8097027369975827 1103 88-89 0.9182949218818415 1103 90-91 0.780910785784247 1103 92-93 0.6462658552159617 1103 94-95 0.5330168230347034 1103 96-97 0.6867861347254873 1103 98-99 0.9341931796809213 1103 100-101 0.9592778027363487 1103 102-103 0.86086083980776 1103 104-105 0.9621053966385595 1103 106-107 0.9974522546759097 1103 108-109 0.7815728705239202 1103 110-111 1.0650537926244823 1103 112-113 0.7685973789501865 1103 114-115 0.8138445362587596 1103 116-117 0.772108884774287 1103 118-119 0.6086462346778099 1103 120-121 0.8028425247409814 1104 1 0.5956109438703372 1104 2 0.705500285330416 1104 3 0.7722336886814958 1104 4 0.9905255340408772 1104 5 1.068710306604153 1104 6 0.9704181220391277 1104 7 0.6533311561840875 1104 8 0.7907623917284567 1104 9 0.9387645972820806 1104 10-11 1.0167586859482682 1104 12-13 1.0999468394902863 1104 14-15 1.2865433463797231 1104 16-17 1.1149439723766577 1104 18-19 1.0215192038930994 1104 20-21 1.0160475049021613 1104 22-23 0.9205919773030118 1104 24-25 0.9899047062512238 1104 26-27 0.922128812111815 1104 28-29 1.1317495827759174 1104 30-31 1.037861917308966 1104 32-33 0.9346839659295298 1104 34-35 0.9037644264873705 1104 36-37 0.9080878074043568 1104 38-39 0.8401330323903551 1104 40-41 0.9913773883221495 1104 42-43 0.8323713764580916 1104 44-45 0.9465619328448227 1104 46-47 1.1262804678316698 1104 48-49 1.205314688703318 1104 50-51 1.0427382343179872 1104 52-53 1.085251983535592 1104 54-55 1.0323600171762592 1104 56-57 0.9804905365812786 1104 58-59 0.9738717480877952 1104 60-61 1.08186846395499 1104 62-63 1.0402633528432617 1104 64-65 0.6740224848457643 1104 66-67 0.8265334873524992 1104 68-69 0.7770083665487668 1104 70-71 0.6715155956896091 1104 72-73 0.6234712123051622 1104 74-75 0.6523339018042797 1104 76-77 0.7318449340907272 1104 78-79 0.6877324904886741 1104 80-81 0.7329131446638399 1104 82-83 0.7382400030085954 1104 84-85 0.826270607129505 1104 86-87 0.9657751181692653 1104 88-89 0.7805404268422151 1104 90-91 1.0771142283033903 1104 92-93 1.0814375014462705 1104 94-95 0.8892940475276347 1104 96-97 0.5679439072582539 1104 98-99 0.5417066600025287 1104 100-101 0.5964834903766665 1104 102-103 0.7693241790897041 1104 104-105 0.7328861998930236 1104 106-107 0.7009082061659413 1104 108-109 0.7017386640879053 1104 110-111 0.5026883559771989 1104 112-113 0.5123062423815767 1104 114-115 0.5529139623136814 1104 116-117 0.5364445591741465 1104 118-119 0.3602226167705673 1104 120-121 0.25851337462489 1105 1 0.7117745074559565 1105 2 0.8151418702887341 1105 3 0.8830338796403296 1105 4 1.029251618283105 1105 5 1.1452333869170346 1105 6 1.0494328479030273 1105 7 0.8772285525338361 1105 8 0.9483291355131129 1105 9 1.0801677774811154 1105 10-11 1.1238324254363548 1105 12-13 1.2846425906562402 1105 14-15 1.3785184850084917 1105 16-17 1.2573946719470044 1105 18-19 1.0311415093537804 1105 20-21 1.2811461057614721 1105 22-23 1.3827316343115612 1105 24-25 1.3758003766268985 1105 26-27 1.4222022578170765 1105 28-29 1.3787823762833185 1105 30-31 1.4098754313187314 1105 32-33 1.3527901684193395 1105 34-35 1.315536854969615 1105 36-37 1.5816207294417381 1105 38-39 1.3987684111865804 1105 40-41 1.296001713539738 1105 42-43 1.3768620304920915 1105 44-45 1.2526652709521287 1105 46-47 1.3052180757050493 1105 48-49 1.2749494799339018 1105 50-51 0.9690978979366633 1105 52-53 0.8634202476463528 1105 54-55 0.9090446780407149 1105 56-57 1.0370804524859523 1105 58-59 1.1248862168917313 1105 60-61 1.0651008094435852 1105 62-63 1.028736049402326 1105 64-65 1.0553277986425442 1105 66-67 0.9503005175754069 1105 68-69 0.8679102798093901 1105 70-71 0.7920785570204494 1105 72-73 0.9777962462737975 1105 74-75 0.5789056398968953 1105 76-77 0.6440405934495494 1105 78-79 1.030793707484012 1105 80-81 0.8846427910227703 1105 82-83 0.8836202681475918 1105 84-85 1.011735702058079 1105 86-87 0.9588344990219717 1105 88-89 0.9613784423392566 1105 90-91 0.9063511274257152 1105 92-93 0.9743426463179254 1105 94-95 0.8974749810225475 1105 96-97 0.8327955469336246 1105 98-99 0.88869171380151 1105 100-101 0.8355088658678369 1105 102-103 0.8228862957949303 1105 104-105 0.7143815544521672 1105 106-107 0.7432019155412846 1105 108-109 0.8854630958017573 1105 110-111 0.761604664596053 1105 112-113 0.7540454366821905 1105 114-115 0.8771262571247398 1105 116-117 1.1043607209015889 1105 118-119 0.9047490755858902 1105 120-121 0.9025982411373192 1106 1 0.32154114887093144 1106 2 0.5968321966943222 1106 3 0.5645224669727611 1106 4 0.7366521485686661 1106 5 0.7599607509695971 1106 6 0.8342793615224764 1106 7 0.7397306110958084 1106 8 0.804607337812115 1106 9 0.8530968641383438 1106 10-11 0.9538306139018218 1106 12-13 1.0000009039529019 1106 14-15 1.0066627325629227 1106 16-17 0.8866867736564288 1106 18-19 0.7533972403903135 1106 20-21 0.8179227270848841 1106 22-23 0.7595287589122037 1106 24-25 0.9381161353304961 1106 26-27 0.9713615411081449 1106 28-29 0.8941777436954581 1106 30-31 0.8119702239803743 1106 32-33 0.7618791312334778 1106 34-35 0.5922849857953452 1106 36-37 0.6248122415790149 1106 38-39 0.7062420204221169 1106 40-41 0.5593986289904791 1106 42-43 0.48020657612629236 1106 44-45 0.4418738773880193 1106 46-47 0.42023857898228556 1106 48-49 0.48717569119177284 1106 50-51 0.2507538463573269 1106 52-53 0.2861325677280391 1106 54-55 0.51564669213775 1106 56-57 0.41437266124059846 1106 58-59 0.5061378637413085 1106 60-61 0.6252451969802308 1106 62-63 0.4971813822306217 1106 64-65 0.1909357743350384 1106 66-67 0.24833479674934011 1106 68-69 0.22131308274736838 1106 70-71 -0.007457031398903524 1106 72-73 0.034045628705207776 1106 74-75 0.05371069407824436 1106 76-77 0.11193958393075931 1106 78-79 0.19512376907618645 1106 80-81 0.17343927335207354 1106 82-83 0.29615341099532344 1106 84-85 0.04917023978740431 1106 86-87 -0.0488311740454499 1106 88-89 0.21182967912329076 1106 90-91 0.2123464833099078 1106 92-93 0.13147082885473083 1106 94-95 0.2731820970595713 1106 96-97 0.43685461942794035 1106 98-99 0.3490794233631185 1106 100-101 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scores pass #Quality Count 2 29078.0 3 4191.0 4 3716.0 5 4156.0 6 4752.0 7 5125.0 8 6197.0 9 7631.0 10 10242.0 11 12347.0 12 12890.0 13 15003.0 14 18442.0 15 18549.0 16 13646.0 17 12892.0 18 13580.0 19 14531.0 20 16250.0 21 17546.0 22 20069.0 23 22168.0 24 24916.0 25 28125.0 26 32425.0 27 37208.0 28 43724.0 29 51957.0 30 61153.0 31 73059.0 32 88978.0 33 110487.0 34 141328.0 35 186773.0 36 255463.0 37 292416.0 38 110291.0 39 503.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.87969847683149 19.24201161045095 13.784239303681789 22.09405060903577 2 14.505354746034183 20.900224923172964 40.51627546740721 24.078144863385646 3 19.234672093423015 26.49430800538979 30.691578731403286 23.57944116978391 4 11.576145224576774 16.8675667255397 40.618455087417026 30.937832962466505 5 11.262813394179341 37.6538730006836 36.030080740530884 15.053232864606175 6 27.31818998423045 37.59290392553047 19.5359405947182 15.552965495520871 7 25.28803548326661 32.87251720785447 24.16804222340224 17.67140508547667 8 23.569429434422368 33.42943442236817 21.877906171197385 21.12322997201208 9 23.72168317073117 18.327990849055976 24.134506463075898 33.81581951713696 10-11 23.281236189389517 25.863326804450203 29.309291453166463 21.546145552993814 12-13 25.387883918143643 23.97658080274203 29.10394970358866 21.531585575525664 14-15 21.943018547345357 25.7887290615716 28.76501024928 23.503242141803042 16-17 19.910935410989733 29.081822132450597 29.27333206214345 21.733910394416224 18-19 19.447502447249338 29.038696064523663 30.942019804768968 20.571781683458035 20-21 20.696946596626425 27.552897476056494 29.911266961427046 21.838888965890032 22-23 20.0817825321823 27.344520348635804 30.306400432330566 22.26729668685133 24-25 20.95351050873384 27.3026174499434 30.229932675934172 21.513939365388584 26-27 20.658552211541874 26.46387733657291 31.675000221686233 21.202570230198987 28-29 19.81297516008784 28.06805579923628 31.07848052988708 21.040488510788798 30-31 20.37132668349223 27.541097209921816 30.356815671338744 21.730760435247205 32-33 20.2994018614913 27.518362685913534 30.758538302484023 21.423697150111146 34-35 21.094824589561362 26.76551123470609 30.68486551007046 21.454798665662086 36-37 21.28261746445606 26.859755125576807 31.091261672980508 20.76636573698663 38-39 20.968819383040117 27.70656569716356 30.797324603832372 20.527290315963956 40-41 20.216870696176723 28.100409075448457 30.787106403138214 20.8956138252366 42-43 19.971494043103203 28.30962502913933 29.61190708565951 22.106973842097954 44-45 20.3830806861686 27.836010382653896 29.41156092880743 22.369348002370074 46-47 21.07070419873295 28.231326732881794 29.28956606007802 21.408403008307236 48-49 21.063794172796367 27.862263072742216 29.498811934199942 21.575130820261467 50-51 20.18101278454766 28.254654218457205 30.182343353254904 21.38198964374023 52-53 20.39033361435225 28.826721074589738 29.06134692866277 21.721598382395246 54-55 20.128335611677095 28.438996375714964 29.285725372545237 22.146942640062708 56-57 20.94964041546075 27.670288211195874 29.018549969660228 22.36152140368315 58-59 21.546642596335612 27.179809753085976 29.23500093008247 22.03854672049594 60-61 21.022197490877545 27.606113965474265 30.184800259734573 21.186888283913618 62-63 20.41052642956859 28.05679666771893 29.81082281833176 21.721854084380723 64-65 19.987224767877358 28.902175253460882 29.67558946888566 21.435010509776102 66-67 20.194497783411432 27.873230588118574 29.038294037394763 22.893977591075227 68-69 20.038162278937765 28.29907052898052 29.21756518118906 22.445202010892658 70-71 20.917585187768005 27.69263859166165 29.060504456491522 22.329271764078822 72-73 20.240583947432754 27.14993006667365 30.57674621270708 22.032739773186517 74-75 19.802859261415218 28.56300977803649 29.932849628905846 21.70128133164245 76-77 20.188668469793196 27.942916518346756 29.427002762354356 22.441412249505692 78-79 20.289783902244544 28.05933178908433 29.454094382301786 22.19678992636934 80-81 20.574474863845367 27.992099367905027 29.00604342294858 22.427382345301027 82-83 20.15549401362473 27.4798745772614 30.003642397057895 22.360989012055974 84-85 20.378668034633595 27.738570428344644 29.456773862588648 22.425987674433117 86-87 20.684379128183163 28.053056986774568 28.951515983216268 22.311047901826 88-89 20.219005987054476 27.191473742457962 28.66389658663597 23.925623683851587 90-91 19.993234569133552 25.96627820893536 29.615645890693926 24.42484133123716 92-93 20.31501673084547 26.38890968115749 30.060158963824225 23.235914624172814 94-95 19.7417562845726 26.47749307335247 30.141443597892692 23.63930704418224 96-97 18.65376175596389 27.047054906308542 30.5637456511632 23.73543768656437 98-99 18.985266962462678 27.78811960601702 29.60704873866983 23.619564692850474 100-101 18.35625357724407 27.28358505299867 30.277960183332368 24.082201186424886 102-103 18.886530614960588 26.754774811638416 30.243421552368925 24.11527302103207 104-105 19.55991697509031 26.768492667685262 29.571387230041534 24.100203127182894 106-107 19.344749617259644 26.410401334761087 30.677994432212337 23.566854615766935 108-109 19.627393824619915 26.41503419432667 30.341089517287873 23.616482463765546 110-111 19.74288981887145 26.622620307485573 29.269892236468852 24.36459763717412 112-113 20.445113597363505 25.606881099929197 28.541167782116617 25.40683752059068 114-115 19.96631638479164 25.572656112467236 29.346191325706698 25.114836177034427 116-117 20.741032643661843 25.440186934548503 28.776295297129646 25.042485124660008 118-119 20.520729952551253 25.474411202684706 29.178552423640685 24.826306421123356 120-121 19.956651893859796 26.75739199429566 28.49777576198796 24.788180349856585 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4994.0 1 5459.5 2 3888.0 3 1672.0 4 1373.5 5 1275.0 6 1351.0 7 1581.0 8 1890.5 9 1979.0 10 1861.5 11 1775.0 12 1806.0 13 1927.0 14 2110.5 15 2305.5 16 2497.5 17 2692.0 18 3026.5 19 3319.0 20 3572.0 21 4100.5 22 4727.0 23 5607.5 24 6911.5 25 8412.5 26 10362.0 27 13118.5 28 16851.5 29 21582.0 30 26793.0 31 31745.5 32 36450.5 33 40358.0 34 43987.5 35 47945.0 36 51586.0 37 55688.5 38 59369.0 39 61297.5 40 62686.0 41 64412.5 42 65922.5 43 67955.0 44 68936.5 45 69834.5 46 72852.0 47 83058.0 48 108217.0 49 107088.5 50 80372.5 51 65058.0 52 53512.5 53 45735.5 54 40550.5 55 35302.5 56 29890.0 57 24256.0 58 19249.0 59 15565.0 60 12487.0 61 9700.5 62 7253.0 63 5117.0 64 3535.0 65 2361.5 66 1471.0 67 901.5 68 565.0 69 354.0 70 203.0 71 126.5 72 92.5 73 74.5 74 64.5 75 58.0 76 41.5 77 26.5 78 26.5 79 25.0 80 22.0 81 15.0 82 8.0 83 9.5 84 11.0 85 11.0 86 8.0 87 4.5 88 5.0 89 3.0 90 1.5 91 3.5 92 3.0 93 2.0 94 2.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4727723628243826 2 0.9924212608690163 3 1.0098215672681023 4 0.9839132246170971 5 0.9950560075792879 6 1.0061987905414789 7 0.9709590532915946 8 0.9582244441919479 9 0.9552603541428922 10-11 0.947493340403237 12-13 0.9498536343311887 14-15 0.9490577212624609 16-17 0.9728253322113702 18-19 1.0018349913025912 20-21 0.9925310419819443 22-23 0.9678302915731469 24-25 0.9591850289300677 26-27 0.9581146630790198 28-29 0.9811961420721296 30-31 0.9882770238559848 32-33 0.9812784779068254 34-35 0.9916527930785204 36-37 1.0006273990603836 38-39 0.985944175206265 40-41 0.9938209700588482 42-43 0.9865754166056009 44-45 0.9684889782507148 46-47 0.9625882434308354 48-49 0.966842261556795 50-51 0.9917076836349844 52-53 0.9692574460412107 54-55 0.9806197912292576 56-57 1.0370472832742437 58-59 0.9992002445923196 60-61 1.0114957292402544 62-63 0.9747190564093782 64-65 0.9624784623179075 66-67 0.9644545223506112 68-69 0.9842700132341132 70-71 0.9806472365074895 72-73 0.9850659263028411 74-75 0.9891003822029447 76-77 1.0030151382665673 78-79 1.0778584120052235 80-81 1.1076365388869402 82-83 1.0661118329219286 84-85 1.023406979992941 86-87 1.003097474101263 88-89 1.0016977649114314 90-91 1.0166828868261018 92-93 1.0018624365808233 94-95 0.9926682683731043 96-97 1.004908862464575 98-99 1.042261886138323 100-101 1.0288136998046447 102-103 1.0005450632256874 104-105 0.9886338124730008 106-107 0.9901433027757605 108-109 0.9879751257954327 110-111 0.985285488528697 112-113 0.9990081276446957 114-115 1.0022741157543031 116-117 1.0332049443217641 118-119 1.0266455228243168 120-121 1.002740685484247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1821807.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.18685538550693 #Duplication Level Percentage of deduplicated Percentage of total 1 81.94737916228428 50.96049817186277 2 11.545195550505758 14.359188121933993 3 2.9023401390492287 5.414622195198195 4 1.1348349030621805 2.8228725601261444 5 0.6271083190666693 1.949894717442365 6 0.3816854284986274 1.4241489926879636 7 0.2552352278965536 1.111059334454292 8 0.1968064077317976 0.979101729324673 9 0.14320151570879533 0.8014726753531429 >10 0.7932063166389532 8.944888009616651 >50 0.04626283410590068 1.9512322346290987 >100 0.02153970056159312 2.414873267094169 >500 0.0019741187512892764 0.8632557959248959 >1k 0.0027817127859076167 3.524784460594181 >5k 2.6919801153944676E-4 1.0441656401646189 >10k+ 1.7946534102629785E-4 1.4339420935928928 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12953 0.7109973778781177 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12744 0.699525251577143 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7553 0.4145883729725487 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6003 0.32950801045335754 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5156 0.28301570912835444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3817 0.20951725402306612 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3675 0.20172279500517892 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3490 0.19156804205933997 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3387 0.18591431474354858 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3085 0.1693373666914223 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3032 0.1664281671988306 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2604 0.14293500903224107 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2598 0.1426056656934571 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2544 0.1396415756444014 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2509 0.1377204061681616 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2319 0.1272912004400027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2250 0.12350375204398709 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2114 0.11603863636488387 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1872 0.10275512170059727 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1823 0.10006548443386154 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.4890556463994266E-5 0.0 6 0.0 0.0 0.0 5.4890556463994266E-5 0.0 7 0.0 0.0 0.0 5.4890556463994266E-5 0.0 8 0.0 0.0 0.0 5.4890556463994266E-5 0.0 9 0.0 0.0 0.0 5.4890556463994266E-5 0.0 10-11 0.0 5.4890556463994266E-5 0.0 1.0978111292798853E-4 0.0 12-13 0.0 5.4890556463994266E-5 0.0 1.3722639115998565E-4 0.0 14-15 0.0 5.4890556463994266E-5 0.0 3.018980605519685E-4 0.0 16-17 0.0 1.0978111292798853E-4 0.0 5.489055646399427E-4 0.0 18-19 0.0 1.0978111292798853E-4 0.0 6.586866775679311E-4 0.0 20-21 0.0 1.0978111292798853E-4 0.0 8.782489034239083E-4 0.0 22-23 0.0 1.0978111292798853E-4 0.0 0.002113286423863779 0.0 24-25 0.0 1.0978111292798853E-4 0.0 0.003979565343639585 0.0 26-27 0.0 1.0978111292798853E-4 0.0 0.006998545949159269 0.0 28-29 0.0 1.0978111292798853E-4 0.0 0.014820450245278453 0.0 30-31 0.0 1.0978111292798853E-4 0.0 0.03405959028590844 0.0 32-33 0.0 1.0978111292798853E-4 0.0 0.07283976842772039 0.0 34-35 0.0 1.0978111292798853E-4 0.0 0.11181206351715631 0.0 36-37 0.0 1.0978111292798853E-4 0.0 0.14213909596351315 0.0 38-39 0.0 1.0978111292798853E-4 0.0 0.16719663498932652 0.0 40-41 0.0 1.0978111292798853E-4 0.0 0.18939986507901221 0.0 42-43 0.0 1.6467166939198278E-4 0.0 0.21882120334371313 0.0 44-45 0.0 1.6467166939198278E-4 0.0 0.24256136901439065 0.0 46-47 0.0 1.6467166939198278E-4 0.0 0.2818630074426105 0.0 48-49 0.0 1.6467166939198278E-4 0.0 0.3219880042177904 0.0 50-51 0.0 1.6467166939198278E-4 0.0 0.3530286138981791 0.0 52-53 0.0 1.6467166939198278E-4 0.0 0.4179366969168523 0.0 54-55 0.0 1.6467166939198278E-4 0.0 0.5541750580604861 0.0 56-57 0.0 1.6467166939198278E-4 0.0 0.851050632695999 0.0 58-59 0.0 1.6467166939198278E-4 0.0 1.1493259165213439 0.0 60-61 0.0 1.6467166939198278E-4 0.0 1.3623561661581056 0.0 62-63 0.0 1.6467166939198278E-4 0.0 1.6452895394517641 0.0 64-65 0.0 1.6467166939198278E-4 0.0 2.0194784628668128 0.0 66-67 0.0 1.6467166939198278E-4 0.0 2.783198220228597 0.0 68-69 0.0 1.6467166939198278E-4 0.0 3.543295200863758 0.0 70-71 0.0 1.6467166939198278E-4 0.0 3.9486894056285875 0.0 72-73 0.0 1.6467166939198278E-4 0.0 4.334570017570467 0.0 74-75 0.0 1.6467166939198278E-4 0.0 4.764445410518238 0.0 76-77 0.0 1.6467166939198278E-4 0.0 5.18773393669033 0.0 78-79 0.0 1.6467166939198278E-4 0.0 5.448464079894302 0.0 80-81 0.0 1.6467166939198278E-4 0.0 5.731424898466193 0.0 82-83 0.0 1.6467166939198278E-4 0.0 6.165307302035836 0.0 84-85 0.0 1.6467166939198278E-4 0.0 7.117466339738512 0.0 86-87 0.0 1.6467166939198278E-4 0.0 8.406571058295416 0.0 88-89 0.0 2.1956222585597706E-4 0.0 9.6954836599047 0.0 90-91 0.0 2.1956222585597706E-4 0.0 11.164931301724057 0.0 92-93 0.0 2.1956222585597706E-4 0.0 12.621040538322665 0.0 94-95 0.0 2.1956222585597706E-4 0.0 13.875811213811343 0.0 96-97 0.0 2.7445278231997134E-4 0.0 15.06284694262345 0.0 98-99 0.0 3.018980605519685E-4 0.0 16.251200044790693 0.0 100-101 0.0 3.2934333878396557E-4 0.0 17.305976977802807 0.0 102-103 0.0 3.2934333878396557E-4 0.0 18.11802787013114 0.0 104-105 0.0 3.8423389524795985E-4 0.0 19.106277448708894 0.0 106-107 0.0 3.8423389524795985E-4 0.0 20.312524872283397 0.0 108-109 0.0 3.8423389524795985E-4 0.0 21.477165254058196 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2280 0.0 52.236893 2 TATAACG 355 0.0 48.62222 2 ACCTGGG 2545 0.0 45.403404 3 TAACGCA 425 0.0 41.930378 4 GTACCTG 3490 0.0 35.031166 1 ATAACGC 570 0.0 30.257046 3 GTATAAC 725 0.0 29.431194 1 GAGTACG 420 0.0 28.834618 1 GTACACG 285 0.0 28.328745 1 TCACGCA 350 0.0 27.92138 4 CCTGGGG 2045 0.0 27.266817 4 TACACGG 270 9.094947E-11 25.571684 2 GTACATG 6875 0.0 24.745342 1 TATCACG 405 0.0 24.151033 2 GAGTACT 5805 0.0 24.110073 12-13 AGTACTT 6115 0.0 24.062754 12-13 TACATGG 6770 0.0 23.626423 2 GTACCGG 225 2.586603E-7 23.067692 1 TACGCAG 380 0.0 22.691525 4 AGTACGC 385 0.0 22.416735 2 >>END_MODULE