FastQCFastQC Report
Wed 25 May 2016
SRR1003812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821807
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT198601.090126451374926No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT73510.40350048056682186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71490.392412588161095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62810.34476758515034794No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57520.315730480780895No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55980.3072773350854399No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43790.2403657467558309No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA43620.23943260729594298No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41560.22812515266436018No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC40370.22159317644514484No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39540.21703726025863332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37010.20314994947324277No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG36470.2001858594241871No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30930.16977649114313426No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA29120.1598413004231513No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27560.15127837361476817No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27440.15061968693720026No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24080.13217645996529817No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC22050.12103367700310737No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21920.12032009976907541No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20400.1119767351865483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22750.052.6298182
TATAACG4800.050.482592
TAACGCA5150.047.954574
ATAACGC5100.047.513033
ACCTGGG26450.044.3694463
GTATAAC7750.039.8660551
GTACCTG32200.038.3803631
TATCACG3500.033.9378782
TCACGCA3300.033.101514
CCTGGGG18150.029.0455934
ACAACGC2151.6589183E-924.308993
TACGCAG4000.023.7467354
TAGACCG1252.2587094E-422.7968655
GTACGCA4600.021.6907313
GTACACG2457.4669515E-921.341171
AGTACGC4500.021.1169012
CACGCAG5400.021.108215
GAGTACG5150.020.305191
AGTACTT87050.020.16004412-13
CTGGGGG12050.020.100975
Produced by FastQC (version 0.11.5)