##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003812_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1821807 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.621357805739027 31.0 31.0 34.0 30.0 34.0 2 31.83369368983652 31.0 31.0 34.0 30.0 34.0 3 31.860514313535955 31.0 31.0 34.0 30.0 34.0 4 35.63899194590865 37.0 35.0 37.0 33.0 37.0 5 35.511562421266355 37.0 35.0 37.0 33.0 37.0 6 35.54582345989449 37.0 35.0 37.0 33.0 37.0 7 35.472350254445175 37.0 35.0 37.0 33.0 37.0 8 35.465260590172285 37.0 35.0 37.0 33.0 37.0 9 37.02253147561734 39.0 37.0 39.0 33.0 39.0 10-11 36.94221341777697 39.0 37.0 39.0 33.0 39.0 12-13 36.89233876036265 39.0 37.0 39.0 33.0 39.0 14-15 37.980589601423205 40.0 37.0 41.0 33.0 41.0 16-17 37.98610912132844 40.0 37.0 41.0 33.0 41.0 18-19 38.012781540525424 40.0 37.0 41.0 33.0 41.0 20-21 37.9562288980117 40.0 37.0 41.0 33.0 41.0 22-23 37.85267676543124 40.0 37.0 41.0 33.0 41.0 24-25 37.7534244845914 39.5 37.0 41.0 32.0 41.0 26-27 37.51476363851934 39.0 36.0 41.0 32.0 41.0 28-29 37.32132520074849 39.0 36.0 41.0 32.0 41.0 30-31 37.075516780866465 39.0 36.0 40.0 31.0 41.0 32-33 36.784929468379474 39.0 35.0 40.0 30.5 41.0 34-35 36.73145399046112 39.0 35.0 40.0 30.0 41.0 36-37 36.52201907227275 38.5 35.0 40.0 30.0 41.0 38-39 36.24561163723709 38.0 35.0 40.0 30.0 41.0 40-41 36.333578145215164 39.0 35.0 40.0 30.0 41.0 42-43 36.174581610455995 39.0 35.0 40.0 30.0 41.0 44-45 35.98096203384881 39.0 35.0 40.0 29.5 41.0 46-47 35.655514277857094 39.0 35.0 40.0 28.0 41.0 48-49 35.36684017571565 38.5 35.0 40.0 27.0 41.0 50-51 35.090005417697924 38.0 34.0 40.0 26.0 41.0 52-53 34.88255287195625 38.0 34.0 40.0 26.0 41.0 54-55 34.66337817342891 38.0 34.0 40.0 25.0 41.0 56-57 34.40301332687821 38.0 34.0 40.0 24.0 41.0 58-59 34.13821881242085 38.0 33.0 40.0 23.0 41.0 60-61 33.69800670433256 37.0 33.0 40.0 22.0 41.0 62-63 33.456065049700655 37.0 32.5 40.0 22.0 41.0 64-65 33.005736612056054 36.0 32.0 39.0 20.5 41.0 66-67 32.662106633688424 36.0 31.5 39.0 20.0 40.5 68-69 32.39072497800261 35.5 31.0 39.0 19.5 40.0 70-71 31.9613888847721 35.0 31.0 38.5 18.0 40.0 72-73 31.588929288338445 35.0 31.0 37.5 17.5 40.0 74-75 31.21260320110747 35.0 31.0 37.0 13.5 39.0 76-77 30.22907640600788 34.0 29.5 36.0 12.5 39.0 78-79 30.592914068284948 34.0 30.0 36.0 10.5 39.0 80-81 30.407752577523304 34.0 30.0 36.0 7.5 37.5 82-83 30.10438921356653 34.0 30.0 35.5 7.0 37.0 84-85 29.75406148949916 34.0 30.0 35.0 3.5 37.0 86-87 29.377279810649537 34.0 29.0 35.0 2.0 36.0 88-89 29.058542974091107 34.0 29.0 35.0 2.0 36.0 90-91 28.764695711455715 34.0 29.0 35.0 2.0 36.0 92-93 28.509909392158445 34.0 29.0 35.0 2.0 35.0 94-95 28.187618666521757 34.0 29.0 35.0 2.0 35.0 96-97 27.763111295543382 34.0 28.5 35.0 2.0 35.0 98-99 27.357244483087396 34.0 27.0 35.0 2.0 35.0 100-101 26.349156908497992 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04545329705808854 1101 2 0.10253658602345794 1101 3 0.18911441631108872 1101 4 0.08677895443959471 1101 5 0.1935894465809156 1101 6 0.20866197354035165 1101 7 0.21901239480295231 1101 8 0.12491711587036747 1101 9 0.22245450008620793 1101 10-11 0.22614025056237352 1101 12-13 0.2866961396815384 1101 14-15 0.22651201749914662 1101 16-17 0.2726450067714268 1101 18-19 0.18668090803512882 1101 20-21 0.014818491899156072 1101 22-23 0.0820225561910064 1101 24-25 0.08764665061328714 1101 26-27 0.022640493290943198 1101 28-29 0.003286813824438184 1101 30-31 0.12075620005223442 1101 32-33 0.2606421691867524 1101 34-35 0.2857807768866536 1101 36-37 0.2527979193528296 1101 38-39 0.07255623136931888 1101 40-41 0.02435658107280858 1101 42-43 0.05513037188158876 1101 44-45 0.2778314793901231 1101 46-47 0.3809567344092457 1101 48-49 0.2825166486406019 1101 50-51 0.2072387125265891 1101 52-53 0.3615490875889691 1101 54-55 0.44416642662541506 1101 56-57 0.40290985880858443 1101 58-59 0.2363058649184424 1101 60-61 0.1811595633666414 1101 62-63 0.04168054268858157 1101 64-65 -0.029057091280435543 1101 66-67 -0.003496275954589123 1101 68-69 -0.23019042453066874 1101 70-71 -0.11668290758899857 1101 72-73 -0.17692691300088725 1101 74-75 -0.22187589339720404 1101 76-77 -0.168000370988441 1101 78-79 -0.1439781501231039 1101 80-81 -0.15826457680331174 1101 82-83 -0.16944364801122447 1101 84-85 -0.2987948441141093 1101 86-87 -0.274029600432371 1101 88-89 -0.14063290688499208 1101 90-91 -0.24036400693332283 1101 92-93 -0.22321976949961098 1101 94-95 -0.22623107123386887 1101 96-97 -0.16090642098370012 1101 98-99 -0.3191246762177613 1101 100-101 -0.46200867397680057 1102 1 0.013454074104988223 1102 2 0.025469722217113855 1102 3 0.08450123997000958 1102 4 -0.004777135935277954 1102 5 -0.07324414127125323 1102 6 0.04827949378944396 1102 7 0.029739958922810672 1102 8 0.037531716797431613 1102 9 0.04143980634520972 1102 10-11 -0.026039258063391912 1102 12-13 0.0502105501241914 1102 14-15 -0.014965828570019823 1102 16-17 0.00692545594770877 1102 18-19 -0.09690100835678095 1102 20-21 -0.07437650909913174 1102 22-23 -0.08913767195859634 1102 24-25 -0.07624872865574872 1102 26-27 -0.07794094284281528 1102 28-29 -0.18211846469830562 1102 30-31 -0.2109179043202687 1102 32-33 -0.10745492929854805 1102 34-35 -0.24697417813635525 1102 36-37 -0.20447124645495762 1102 38-39 -0.177224414766755 1102 40-41 -0.12429356974983818 1102 42-43 0.10947427988786984 1102 44-45 0.08608949133722632 1102 46-47 -0.05853127802350144 1102 48-49 -0.11511983371126888 1102 50-51 -0.01880611876249816 1102 52-53 -0.28880678909800395 1102 54-55 -0.38233977625937143 1102 56-57 -0.3431280114353754 1102 58-59 -0.4725624526131327 1102 60-61 -0.4080554034200006 1102 62-63 -0.589221641136966 1102 64-65 -0.5162514663242845 1102 66-67 -0.49587716923475256 1102 68-69 -0.36692019028340184 1102 70-71 -0.34863383491892463 1102 72-73 -0.41092703171638334 1102 74-75 -0.3271339107404607 1102 76-77 -0.29632022291783855 1102 78-79 -0.37700156405706764 1102 80-81 -0.3520687070234203 1102 82-83 -0.5371751567233254 1102 84-85 -0.5172391854319613 1102 86-87 -0.6433664664511376 1102 88-89 -0.6291010452640968 1102 90-91 -0.4756494907259601 1102 92-93 -0.43335645420743774 1102 94-95 -0.4819876936700176 1102 96-97 -0.45095698299366305 1102 98-99 -0.4518146075246534 1102 100-101 -0.40164616001424136 1103 1 0.11345288376696061 1103 2 0.01978317988125511 1103 3 -0.052443578934941115 1103 4 -0.06469785942272921 1103 5 0.09243335449560419 1103 6 0.03386509546067629 1103 7 0.046856424571139144 1103 8 0.059078322994920995 1103 9 0.04036016975540946 1103 10-11 0.07113487946231345 1103 12-13 0.08182994549198952 1103 14-15 0.03624072781182974 1103 16-17 -0.026967336257960994 1103 18-19 0.18132145986863435 1103 20-21 0.013096819889462097 1103 22-23 0.018872261412226976 1103 24-25 0.13949096227297986 1103 26-27 0.16834639285333708 1103 28-29 -0.03688160231030935 1103 30-31 0.2581123272897514 1103 32-33 0.11090008306609178 1103 34-35 -0.051666937012555536 1103 36-37 -0.03920545885291915 1103 38-39 0.009705376120606957 1103 40-41 0.15624544506689375 1103 42-43 0.28136444739818955 1103 44-45 0.3278571135477435 1103 46-47 0.39940574714321286 1103 48-49 0.41936009272584585 1103 50-51 0.3842613823311325 1103 52-53 0.3207935846681309 1103 54-55 0.1902992626617035 1103 56-57 0.2464248380850691 1103 58-59 0.2977252223293547 1103 60-61 0.40807337925105713 1103 62-63 0.10566808892412638 1103 64-65 0.2655664179093904 1103 66-67 0.17296513775937683 1103 68-69 0.1643900147820787 1103 70-71 0.24177204058957358 1103 72-73 0.24135782212498214 1103 74-75 0.44549129050106373 1103 76-77 0.3636856097384893 1103 78-79 0.3900147644048104 1103 80-81 0.17543375548837048 1103 82-83 0.15754123036269974 1103 84-85 -0.04095101649918931 1103 86-87 0.051691398856160475 1103 88-89 0.15579341639065447 1103 90-91 0.09848937410818337 1103 92-93 0.15002240955539747 1103 94-95 -0.09756177882085026 1103 96-97 0.01221946378781169 1103 98-99 0.05518428248513629 1103 100-101 0.12881935142937095 1104 1 -0.11666148308967905 1104 2 -0.07898967909827093 1104 3 -0.1498923920909654 1104 4 -0.09229941757520521 1104 5 -0.007318147049112156 1104 6 -0.04209483776136125 1104 7 -0.10607662476395774 1104 8 -0.1856811420138058 1104 9 -0.2406119485260163 1104 10-11 -0.07152146348474275 1104 12-13 -0.18347621611835763 1104 14-15 -0.04863876482916396 1104 16-17 -0.23819102324585373 1104 18-19 -0.31179590048291317 1104 20-21 -0.25344559754956464 1104 22-23 -0.1467687494679737 1104 24-25 -0.18348027331123262 1104 26-27 -0.10549026978362264 1104 28-29 -0.18923322282726218 1104 30-31 -0.14468304046877734 1104 32-33 -0.2353331025114116 1104 34-35 -0.2413945481652462 1104 36-37 -0.3462147463627474 1104 38-39 -0.08072647389762011 1104 40-41 -0.07975948652406828 1104 42-43 -0.01825944683165659 1104 44-45 -0.03534544627418512 1104 46-47 -0.1298494291083898 1104 48-49 -0.15961396365164404 1104 50-51 -0.07365890627248461 1104 52-53 -0.10318843517336518 1104 54-55 -0.26197128403489245 1104 56-57 -0.3103186808065672 1104 58-59 -0.27052022506380524 1104 60-61 -0.1562498222533648 1104 62-63 -0.39423116845887307 1104 64-65 -0.2843472312760227 1104 66-67 -0.23248359864037127 1104 68-69 -0.2824620182998814 1104 70-71 -0.38519723770950876 1104 72-73 -0.37652878966094505 1104 74-75 -0.327189230674076 1104 76-77 -0.30812180875593853 1104 78-79 -0.20828652318177987 1104 80-81 -0.19410263658270566 1104 82-83 -0.27309316028838637 1104 84-85 -0.47796049589972256 1104 86-87 -0.43521445956688254 1104 88-89 -0.533409853426857 1104 90-91 -0.5754835309251085 1104 92-93 -0.7706605294425444 1104 94-95 -0.7597137370654323 1104 96-97 -0.7212793752734576 1104 98-99 -0.7018499508155784 1104 100-101 -0.5921787680417765 1105 1 0.141090711100766 1105 2 0.07655547164904064 1105 3 0.14683743788222614 1105 4 0.0989678880690974 1105 5 0.05989385397912628 1105 6 0.17100420377218484 1105 7 0.1648205145258217 1105 8 0.13864223942645992 1105 9 0.16346061583077898 1105 10-11 0.19419958972667217 1105 12-13 0.13408832429514916 1105 14-15 0.1360652489786247 1105 16-17 0.2379978791080788 1105 18-19 0.28649924108368197 1105 20-21 0.10971311861951705 1105 22-23 0.2553336338451686 1105 24-25 0.24722003148844607 1105 26-27 0.2847873549822637 1105 28-29 0.4232966144904964 1105 30-31 0.49695057563052103 1105 32-33 0.3512575478503166 1105 34-35 0.25861004219133576 1105 36-37 0.31608281891212187 1105 38-39 0.33523052364194683 1105 40-41 0.31101848710892455 1105 42-43 0.45888057740543076 1105 44-45 0.5129239892955724 1105 46-47 0.3318789319506905 1105 48-49 0.22842632131769136 1105 50-51 0.37723529518908094 1105 52-53 0.4275862334028915 1105 54-55 0.1333621594669907 1105 56-57 0.2642296281060652 1105 58-59 0.1596632055850833 1105 60-61 0.30676751093307786 1105 62-63 0.12748489044458466 1105 64-65 0.07790718464699609 1105 66-67 -0.05346887277647028 1105 68-69 0.16809800520275076 1105 70-71 0.2611268047421049 1105 72-73 0.2953405871522463 1105 74-75 0.09978003229827337 1105 76-77 -0.06905603812687389 1105 78-79 0.141477532490061 1105 80-81 0.29564617910529734 1105 82-83 0.3566725479118311 1105 84-85 0.24310832272610838 1105 86-87 0.23292387541897952 1105 88-89 0.2904655092113124 1105 90-91 0.3948866354294118 1105 92-93 0.35538882607139044 1105 94-95 0.48362198325332173 1105 96-97 0.5248105589926375 1105 98-99 0.37595016669754955 1105 100-101 0.4062026722285026 1106 1 -0.12204126742431498 1106 2 -0.10225369142200691 1106 3 -0.023442279518388176 1106 4 -0.13787501894977083 1106 5 -0.13585233356410242 1106 6 -0.17289380683352817 1106 7 -0.09798621120784645 1106 8 -0.07226278675844355 1106 9 -0.08182980609208101 1106 10-11 -0.15241819295506076 1106 12-13 -0.16257474674994654 1106 14-15 -0.10571676577714584 1106 16-17 -0.164544856638436 1106 18-19 -0.07616667901117324 1106 20-21 -0.025766814518398462 1106 22-23 -0.10214038710276441 1106 24-25 -0.20916089305290342 1106 26-27 -0.17328488407441256 1106 28-29 -0.24366058388900314 1106 30-31 -0.24233605030406125 1106 32-33 -0.0754096814899583 1106 34-35 -0.23208361085039542 1106 36-37 -0.36561574174135103 1106 38-39 -0.08519308724043384 1106 40-41 -0.3472237891665628 1106 42-43 -0.3031576871444841 1106 44-45 -0.293313659332604 1106 46-47 -0.4078659298786249 1106 48-49 -0.22919344581777779 1106 50-51 -0.014707904969014862 1106 52-53 -0.20665300443512535 1106 54-55 -0.2241454160410612 1106 56-57 -0.24337058269068024 1106 58-59 -0.3040453761480606 1106 60-61 -0.32554120748857684 1106 62-63 -0.3553788162844427 1106 64-65 -0.25303650133172084 1106 66-67 -0.6047048333838205 1106 68-69 -0.39169519166165045 1106 70-71 -0.4564216126354488 1106 72-73 -0.34295401776854106 1106 74-75 -0.3750651638415121 1106 76-77 -0.2784626370531278 1106 78-79 -0.3177063300309335 1106 80-81 -0.3674296006779798 1106 82-83 -0.384068246199039 1106 84-85 -0.3733420576961457 1106 86-87 -0.4695824237806505 1106 88-89 -0.5772827945336267 1106 90-91 -0.7131701644124924 1106 92-93 -0.4266261355570151 1106 94-95 -0.41297686065827577 1106 96-97 -0.5067945429473539 1106 98-99 -0.6846781887584612 1106 100-101 -0.6110013477313139 1107 1 -0.2153604366220101 1107 2 -0.1941345034670512 1107 3 -0.25334991805156903 1107 4 -0.3037293219372259 1107 5 -0.23307806275256127 1107 6 -0.3405552996227996 1107 7 -0.30544600935088084 1107 8 -0.16206000996261594 1107 9 -0.14503590869428962 1107 10-11 -0.2223805161137804 1107 12-13 -0.2848581004036461 1107 14-15 -0.21339448179223552 1107 16-17 -0.32266855354997404 1107 18-19 -0.10076033184315492 1107 20-21 -0.10323948122353954 1107 22-23 -0.2922120704946565 1107 24-25 -0.2109128481364806 1107 26-27 -0.14917526773251666 1107 28-29 -0.2658883871974922 1107 30-31 -0.3347072113784222 1107 32-33 -0.31268202427165903 1107 34-35 -0.5631690790243766 1107 36-37 -0.532492225188065 1107 38-39 -0.5480742293411041 1107 40-41 -0.5457136945145464 1107 42-43 -0.5622714156547985 1107 44-45 -0.3864242892879517 1107 46-47 -0.6736078245782622 1107 48-49 -0.851792200985038 1107 50-51 -0.676338217421673 1107 52-53 -0.7154747780061541 1107 54-55 -0.8806788039628302 1107 56-57 -0.9099476714197365 1107 58-59 -0.8598984977888833 1107 60-61 -0.6378234248852195 1107 62-63 -0.860969020666424 1107 64-65 -0.7463080108582432 1107 66-67 -0.8102636554279314 1107 68-69 -0.7175761966900396 1107 70-71 -0.788901701083141 1107 72-73 -0.7204380019547258 1107 74-75 -0.725758280648737 1107 76-77 -0.8258988894248382 1107 78-79 -0.8465913822987581 1107 80-81 -0.9178291393215403 1107 82-83 -0.7551668715083508 1107 84-85 -0.7531459000485938 1107 86-87 -0.7751726732522002 1107 88-89 -1.0238947194612997 1107 90-91 -0.8031143796015421 1107 92-93 -0.8364323496206296 1107 94-95 -0.9093322710454679 1107 96-97 -0.9074148851951911 1107 98-99 -0.8020359582317411 1107 100-101 -0.6527297119540059 1108 1 -0.15864723119227264 1108 2 -0.17586963614734685 1108 3 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-0.4096557092108277 2316 80-81 -0.5465036315896441 2316 82-83 -0.4672994680075 2316 84-85 -0.4973161147447662 2316 86-87 -0.5049287856239246 2316 88-89 -0.5459216451002824 2316 90-91 -0.6684635046078959 2316 92-93 -0.44835669181614435 2316 94-95 -0.734925236125104 2316 96-97 -0.5169768414906883 2316 98-99 -0.811124815474205 2316 100-101 -1.2803821376063418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 23.0 5 50.0 6 131.0 7 328.0 8 765.0 9 1350.0 10 2486.0 11 4417.0 12 6775.0 13 9439.0 14 10206.0 15 12684.0 16 15852.0 17 20021.0 18 17370.0 19 12080.0 20 10771.0 21 10954.0 22 11620.0 23 12887.0 24 14778.0 25 17883.0 26 22018.0 27 27283.0 28 34791.0 29 42663.0 30 53220.0 31 64829.0 32 79865.0 33 100007.0 34 128589.0 35 169988.0 36 235363.0 37 317250.0 38 292211.0 39 60812.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80157416056943 20.395220623568363 14.189357281838117 20.613847934024086 2 15.489724421940409 22.44010807499377 39.352625956662415 22.71754154640341 3 20.345270719263656 27.374196134857133 30.110989680870343 22.16954346500887 4 12.718196823263936 17.666690269605947 39.04535442008951 30.569758487040616 5 12.032449101359255 38.76420498988092 34.491798527505935 14.711547381253887 6 27.922214793614618 38.783918638083726 18.462267247635015 14.83159932066664 7 26.51598111106171 33.38108811745701 22.858952677204556 17.243978094276727 8 24.958351790282947 33.93954463892169 20.562496466420427 20.53960710437494 9 24.748560083477557 18.901069103368247 23.050850062602677 33.299520750551515 10-11 24.160609463837375 26.738831637755666 28.34334542029358 20.757213478113382 12-13 26.46185902238821 24.520627047760822 28.369497976459634 20.64801595339133 14-15 22.50452928507695 26.66944054786715 28.150951101835563 22.675079065220334 16-17 20.357754690809728 29.523846378897435 28.84227033928402 21.276128591008817 18-19 19.96759261546366 29.346247983458184 30.853295656455376 19.832863744622784 20-21 21.51569840291721 27.598090143156917 30.216444037598762 20.669767416327108 22-23 20.743827419699233 27.740863878555743 30.278426858607965 21.236881843137063 24-25 21.50074074805948 27.841670385501864 30.432971220332337 20.22461764610631 26-27 21.45214675447262 26.712461680047717 31.706976324177532 20.128415241302136 28-29 20.619442671771964 28.5268885823881 31.042729412410537 19.8109393334294 30-31 21.29525150022905 27.928071348953154 30.383278570363988 20.393398580453805 32-33 21.03141808596395 28.059228014626953 30.625916208739557 20.28343769066954 34-35 21.767806767485638 27.1401249724842 30.870580524252052 20.22148773577811 36-37 21.963581562668562 27.228841579660358 31.037117049851286 19.770459807819794 38-39 21.456978145629744 27.97615208166987 30.986319094501535 19.58055067819885 40-41 20.901742614762075 28.33918816319741 30.51519208947432 20.243877132566194 42-43 20.5698518009866 28.750465883597986 29.599485565704818 21.080196749710588 44-45 21.28370348780085 28.04204836187368 29.32695395286109 21.347294197464386 46-47 21.85801993078293 28.75458679715996 28.94302628437182 20.44436698768529 48-49 22.01863314829727 28.36705534669699 29.1836347099336 20.430676795072145 50-51 20.97079987067785 28.780792916044344 29.737699986881154 20.510707226396647 52-53 21.327725714084973 29.47279816138592 28.491355011809706 20.708121112719404 54-55 20.944068169679884 29.13733452555622 28.53886278842929 21.379734516334604 56-57 21.711444845182708 28.433125578247388 28.480579258761985 21.37485031780792 58-59 22.597625867135946 27.839413942898293 28.531832767776084 21.031127422189677 60-61 21.73718400731656 28.557841767810583 29.47845064325967 20.226523581613183 62-63 21.190494359409044 28.5742637092245 29.33112786016583 20.904114071200624 64-65 20.82501270574591 29.584193310750834 29.011219770827058 20.579574212676203 66-67 21.17328493097888 28.251305727081217 28.62865302989625 21.946756312043654 68-69 21.067986772230466 28.879084035419332 28.409404352407147 21.64352483994306 70-71 21.753114427543917 28.57917991704374 28.42194561597889 21.24576003943345 72-73 21.287106702301617 27.657924247738645 29.72831370172581 21.326655348233924 74-75 20.586105055613753 29.413881221743367 29.127654336506097 20.872359386136782 76-77 21.071880830406293 28.563536093559854 28.508892544599952 21.8556905314339 78-79 21.0478662119533 29.054971245581996 28.396119896344672 21.501042646120034 80-81 21.33977419122882 28.870237077802425 27.990314012406365 21.799674718562397 82-83 20.771245252653 28.311066979103718 28.955042987539294 21.962644780703993 84-85 20.599964924924794 28.641515989760713 28.588299580663477 22.170219504651016 86-87 21.178931317152212 28.549660389703774 27.768891543171183 22.502516749972827 88-89 20.82100354208761 28.448073808037844 27.533844144851788 23.197078505022763 90-91 20.877870158584308 26.974948498935397 28.147026551111065 24.00015479136923 92-93 20.911929748870214 27.860607627481944 28.39299113462623 22.834471489021617 94-95 20.494833284892376 28.187010084226884 28.554118428119796 22.764038202760943 96-97 19.800801678557363 28.70980800608189 28.83800521736028 22.65138509800047 98-99 20.247249904695934 29.478117938819317 27.550437053892562 22.724195102592187 100-101 19.26737117832979 29.325160749200506 27.342020066067178 24.065448006402523 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 468.0 1 351.0 2 767.5 3 1121.5 4 745.5 5 542.5 6 710.0 7 1151.0 8 1651.5 9 1989.0 10 2012.5 11 1967.5 12 2079.0 13 2276.0 14 2479.5 15 2704.0 16 3002.5 17 3360.0 18 3667.0 19 3890.5 20 4177.5 21 4607.5 22 5220.5 23 6016.0 24 7071.5 25 8426.0 26 10283.5 27 13061.0 28 16929.5 29 21494.5 30 26402.0 31 31263.5 32 35968.0 33 39840.0 34 43141.0 35 46753.5 36 50042.0 37 54009.5 38 57956.5 39 59969.0 40 61529.5 41 63200.5 42 64570.5 43 66807.5 44 68304.0 45 69389.0 46 72310.5 47 83786.0 48 112866.5 49 111358.0 50 82685.0 51 67779.5 52 55154.0 53 46877.0 54 41302.5 55 36067.0 56 30963.0 57 25386.5 58 20426.0 59 16552.0 60 13030.5 61 10017.0 62 7523.0 63 5310.0 64 3540.5 65 2311.5 66 1434.0 67 829.0 68 469.0 69 277.0 70 150.5 71 92.5 72 55.0 73 37.5 74 28.0 75 17.5 76 8.0 77 6.5 78 8.0 79 6.5 80 2.5 81 0.5 82 1.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04940150081759484 2 0.005983070654575375 3 0.05033464027748274 4 0.0 5 0.0 6 0.0011527016857438795 7 0.0 8 0.0 9 0.0 10-11 4.940150081759484E-4 12-13 0.0 14-15 0.0028268636578957047 16-17 0.0 18-19 0.0 20-21 0.011225118796886827 22-23 0.0 24-25 0.0 26-27 0.0076023420702632065 28-29 0.009303949320647029 30-31 0.009249058764183034 32-33 0.005571391481095418 34-35 0.006916210114463277 36-37 0.018635343919526055 38-39 0.009907745441750965 40-41 0.012844390212574658 42-43 0.0 44-45 0.0 46-47 1.0978111292798852E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0016741619721518252 58-59 2.7445278231997134E-4 60-61 0.01023708878053493 62-63 0.015259574696990406 64-65 0.042842079320147525 66-67 0.022395347037309662 68-69 0.0018388336415438079 70-71 1.9211694762397995E-4 72-73 0.0 74-75 2.744527823199713E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.744527823199713E-5 86-87 7.135772340319255E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.233583469599139E-5 96-97 2.470075040879742E-4 98-99 3.5678861701596276E-4 100-101 0.0013722639115998567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1821807.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.63108849307094 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8789287234833 46.833885387182555 2 12.758671443090908 14.961095888677603 3 3.2652034704049773 5.743273008635897 4 1.309542864360516 3.0711969426316403 5 0.6822865077307769 2.0001600306195755 6 0.4294398501747099 1.5107115514826728 7 0.2921162283536476 1.1988964704405383 8 0.20742493391338748 0.972923972475617 9 0.15808249294853566 0.8341693769943769 >10 0.9232853384205465 9.89005340041545 >50 0.061345790059643246 2.445847670182595 >100 0.027825189653979718 3.0390250984140863 >500 0.0027349653991581334 1.0886930597881777 >1k 0.0025463470957679175 3.5352527186529095 >5k 4.7154575847554027E-4 1.7766670071640984 >10k+ 9.430915169510805E-5 1.098148416242227 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19860 1.090126451374926 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7351 0.40350048056682186 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7149 0.392412588161095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6281 0.34476758515034794 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5752 0.315730480780895 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5598 0.3072773350854399 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4379 0.2403657467558309 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4362 0.23943260729594298 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4156 0.22812515266436018 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4037 0.22159317644514484 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3954 0.21703726025863332 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3701 0.20314994947324277 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3647 0.2001858594241871 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3093 0.16977649114313426 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2912 0.1598413004231513 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2756 0.15127837361476817 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2744 0.15061968693720026 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2408 0.13217645996529817 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2205 0.12103367700310737 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2192 0.12032009976907541 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2040 0.1119767351865483 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.4890556463994266E-5 0.0 12-13 2.7445278231997133E-5 0.0 0.0 8.233583469599139E-5 0.0 14-15 8.233583469599139E-5 0.0 0.0 2.470075040879742E-4 0.0 16-17 1.0978111292798853E-4 0.0 0.0 4.940150081759484E-4 0.0 18-19 1.0978111292798853E-4 0.0 0.0 6.03796121103937E-4 0.0 20-21 1.0978111292798853E-4 0.0 0.0 7.959130687279169E-4 0.0 22-23 1.0978111292798853E-4 0.0 0.0 0.0020309505891677876 0.0 24-25 1.0978111292798853E-4 0.0 0.0 0.0038423389524795985 0.0 26-27 1.0978111292798853E-4 0.0 0.0 0.006833874279767286 0.0 28-29 1.0978111292798853E-4 0.0 0.0 0.015149793584062418 0.0 30-31 1.0978111292798853E-4 0.0 0.0 0.03570630697982827 0.0 32-33 1.0978111292798853E-4 0.0 0.0 0.07585874903324008 0.0 34-35 1.0978111292798853E-4 2.7445278231997133E-5 0.0 0.11656009665129183 0.0 36-37 1.0978111292798853E-4 5.4890556463994266E-5 0.0 0.14782026855753655 0.0 38-39 1.0978111292798853E-4 5.4890556463994266E-5 0.0 0.17381094704323785 0.0 40-41 1.6467166939198278E-4 5.4890556463994266E-5 0.0 0.1971119882622034 0.0 42-43 1.6467166939198278E-4 5.4890556463994266E-5 0.0 0.2278781451602722 0.0 44-45 1.6467166939198278E-4 5.4890556463994266E-5 0.0 0.2527710125166936 0.0 46-47 1.6467166939198278E-4 5.4890556463994266E-5 0.0 0.29352725069120933 0.0 48-49 1.6467166939198278E-4 5.4890556463994266E-5 0.0 0.33527151888207696 0.0 50-51 1.6467166939198278E-4 5.4890556463994266E-5 0.0 0.36677869829240967 0.0 52-53 1.6467166939198278E-4 5.4890556463994266E-5 0.0 0.4325375849362748 0.0 54-55 1.6467166939198278E-4 5.4890556463994266E-5 0.0 0.5721242700242122 0.0 56-57 1.6467166939198278E-4 5.4890556463994266E-5 0.0 0.8744065644714287 0.0 58-59 1.6467166939198278E-4 5.4890556463994266E-5 0.0 1.1797078395241647 0.0 60-61 1.6467166939198278E-4 5.4890556463994266E-5 0.0 1.397541012851526 0.0 62-63 1.6467166939198278E-4 5.4890556463994266E-5 0.0 1.6855792078963359 0.0 64-65 1.6467166939198278E-4 5.4890556463994266E-5 0.0 2.06739791865988 0.0 66-67 1.6467166939198278E-4 5.4890556463994266E-5 0.0 2.8453617754240703 0.0 68-69 1.6467166939198278E-4 5.4890556463994266E-5 0.0 3.621761251329038 0.0 70-71 1.6467166939198278E-4 5.4890556463994266E-5 0.0 4.036843639309762 0.0 72-73 1.6467166939198278E-4 5.4890556463994266E-5 0.0 4.435266743403664 0.0 74-75 1.6467166939198278E-4 5.4890556463994266E-5 0.0 4.881362295786547 0.0 76-77 1.6467166939198278E-4 5.4890556463994266E-5 0.0 5.319828060820932 0.0 78-79 1.6467166939198278E-4 5.4890556463994266E-5 0.0 5.591591205874168 0.0 80-81 1.6467166939198278E-4 5.4890556463994266E-5 0.0 5.886161377138192 0.0 82-83 1.6467166939198278E-4 5.4890556463994266E-5 0.0 6.333656638710906 0.0 84-85 1.6467166939198278E-4 5.4890556463994266E-5 0.0 7.316773950259275 0.0 86-87 1.6467166939198278E-4 5.4890556463994266E-5 0.0 8.648034616180528 0.0 88-89 1.6467166939198278E-4 5.4890556463994266E-5 0.0 9.982451489098462 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2275 0.0 52.629818 2 TATAACG 480 0.0 50.48259 2 TAACGCA 515 0.0 47.95457 4 ATAACGC 510 0.0 47.51303 3 ACCTGGG 2645 0.0 44.369446 3 GTATAAC 775 0.0 39.866055 1 GTACCTG 3220 0.0 38.380363 1 TATCACG 350 0.0 33.937878 2 TCACGCA 330 0.0 33.10151 4 CCTGGGG 1815 0.0 29.045593 4 ACAACGC 215 1.6589183E-9 24.30899 3 TACGCAG 400 0.0 23.746735 4 TAGACCG 125 2.2587094E-4 22.796865 5 GTACGCA 460 0.0 21.690731 3 GTACACG 245 7.4669515E-9 21.34117 1 AGTACGC 450 0.0 21.116901 2 CACGCAG 540 0.0 21.10821 5 GAGTACG 515 0.0 20.30519 1 AGTACTT 8705 0.0 20.160044 12-13 CTGGGGG 1205 0.0 20.10097 5 >>END_MODULE