##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003811_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 916865 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.604786964275004 31.0 31.0 34.0 30.0 34.0 2 31.81150005726034 31.0 31.0 34.0 30.0 34.0 3 31.853596767244905 31.0 31.0 34.0 30.0 34.0 4 35.585318449280976 37.0 35.0 37.0 33.0 37.0 5 35.466665212435856 37.0 35.0 37.0 33.0 37.0 6 35.51168710769851 37.0 35.0 37.0 33.0 37.0 7 35.449728149727605 37.0 35.0 37.0 33.0 37.0 8 35.442608235672644 37.0 35.0 37.0 33.0 37.0 9 36.990778358864176 39.0 37.0 39.0 33.0 39.0 10-11 36.90734404737884 39.0 37.0 39.0 33.0 39.0 12-13 36.86346245085154 39.0 37.0 39.0 33.0 39.0 14-15 37.89716534058994 40.0 37.0 41.0 33.0 41.0 16-17 37.933244807032665 40.0 37.0 41.0 33.0 41.0 18-19 37.98787498704826 40.0 37.0 41.0 33.0 41.0 20-21 37.91174873072917 40.0 37.0 41.0 32.5 41.0 22-23 37.823732501513305 40.0 37.0 41.0 32.0 41.0 24-25 37.71811117230999 39.5 37.0 41.0 32.0 41.0 26-27 37.414974941785324 39.0 36.0 41.0 31.5 41.0 28-29 37.2160432560955 39.0 36.0 41.0 31.0 41.0 30-31 36.99898185665283 39.0 36.0 40.0 31.0 41.0 32-33 36.72607035932225 39.0 35.0 40.0 30.0 41.0 34-35 36.655711037066524 39.0 35.0 40.0 30.0 41.0 36-37 36.40403276382019 38.5 35.0 40.0 30.0 41.0 38-39 36.12795231577168 38.0 35.0 40.0 30.0 41.0 40-41 36.27604063848004 39.0 35.0 40.0 30.0 41.0 42-43 36.140275285892685 39.0 35.0 40.0 30.0 41.0 44-45 35.97657943099584 39.0 35.0 40.0 29.5 41.0 46-47 35.65809579381916 39.0 35.0 40.0 27.5 41.0 48-49 35.37694098913144 39.0 35.0 40.0 27.0 41.0 50-51 35.07138019228567 38.0 34.0 40.0 26.0 41.0 52-53 34.84909664999755 38.0 34.0 40.0 25.5 41.0 54-55 34.65855878455389 38.0 34.0 40.0 24.5 41.0 56-57 34.401062860944634 38.0 33.5 40.0 23.5 41.0 58-59 34.12648208842087 38.0 33.0 40.0 23.0 41.0 60-61 33.65081391480752 37.0 33.0 40.0 22.0 41.0 62-63 33.41089909637733 37.0 32.0 40.0 22.0 41.0 64-65 32.93702289868192 36.0 32.0 39.5 19.5 41.0 66-67 32.589287408724296 36.0 31.0 39.0 19.0 41.0 68-69 32.31001019779357 35.5 31.0 39.0 18.5 40.0 70-71 31.87485235012788 35.0 31.0 38.5 17.0 40.0 72-73 31.486184989066004 35.0 31.0 37.5 14.5 40.0 74-75 31.129681032649298 35.0 30.5 37.0 12.0 39.5 76-77 30.142300665855934 34.0 29.0 36.0 9.5 39.0 78-79 30.51182834986612 34.0 30.0 36.0 8.0 39.0 80-81 30.310488457951827 34.0 30.0 36.0 7.0 38.5 82-83 29.996308616862898 34.0 30.0 35.5 4.5 37.0 84-85 29.67012537287387 34.0 30.0 35.0 2.0 37.0 86-87 29.308076434371472 34.0 29.0 35.0 2.0 36.5 88-89 28.975741248711643 34.0 29.0 35.0 2.0 36.0 90-91 28.679446265262605 34.0 29.0 35.0 2.0 36.0 92-93 28.438944664699818 34.0 29.0 35.0 2.0 35.0 94-95 28.12025707165177 34.0 29.0 35.0 2.0 35.0 96-97 27.689603703925876 34.0 27.0 35.0 2.0 35.0 98-99 27.314319447246866 34.0 27.0 35.0 2.0 35.0 100-101 26.332351545756463 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1549269606989654 1101 2 0.17494856618939636 1101 3 0.1428796737507696 1101 4 0.2652595413406402 1101 5 0.2820561512785176 1101 6 0.19815306837435287 1101 7 0.20336029764003882 1101 8 0.12510277609603548 1101 9 0.29122059959581037 1101 10-11 0.32525087600392766 1101 12-13 0.2808054188177991 1101 14-15 0.3129632803896456 1101 16-17 0.438395479062045 1101 18-19 0.14466219666412883 1101 20-21 0.2512557112918046 1101 22-23 0.4353298352728885 1101 24-25 0.1831053704191774 1101 26-27 0.28659572924133414 1101 28-29 0.3910952204256475 1101 30-31 0.36802070354730887 1101 32-33 0.45969408060133077 1101 34-35 0.23928753046016737 1101 36-37 0.0890676375728745 1101 38-39 -0.0074894751721146235 1101 40-41 0.27169281654684596 1101 42-43 0.05926796298913217 1101 44-45 0.3201155216558149 1101 46-47 0.13841666974607136 1101 48-49 -0.018693909374967177 1101 50-51 -0.02647538520689352 1101 52-53 0.0736683901616928 1101 54-55 0.12115183012906527 1101 56-57 0.2833173707585388 1101 58-59 0.1559925784202818 1101 60-61 0.2040784133115494 1101 62-63 -0.007656918464000739 1101 64-65 -0.05487993313220585 1101 66-67 0.03510633142845876 1101 68-69 -0.07292600704193575 1101 70-71 0.037873165202856995 1101 72-73 0.03026625254232229 1101 74-75 -0.04785978697941928 1101 76-77 -0.1623960823959152 1101 78-79 0.0042025411840320714 1101 80-81 -0.06528621179699812 1101 82-83 -0.03680352359209138 1101 84-85 -0.08083346904704669 1101 86-87 -0.1837661148184857 1101 88-89 -0.1385582817388702 1101 90-91 -0.30728652053168304 1101 92-93 0.07577882283617754 1101 94-95 -0.11969438012929956 1101 96-97 0.01692454725971615 1101 98-99 -0.16562753659630758 1101 100-101 -0.32533450367595407 1102 1 0.05911389615654983 1102 2 0.10141199347114949 1102 3 -0.0082169398589933 1102 4 -3.7726497285461846E-4 1102 5 -0.15569145720992594 1102 6 0.046742360526444315 1102 7 0.0824996991818665 1102 8 0.09973620935348038 1102 9 0.19668020934853558 1102 10-11 -0.004766112741023676 1102 12-13 0.03626922637086949 1102 14-15 0.15624951604922188 1102 16-17 0.03382580298406879 1102 18-19 0.028911062023382783 1102 20-21 -0.06006649704749378 1102 22-23 -0.12871575777752753 1102 24-25 -0.1622390220603407 1102 26-27 -0.09896136825134505 1102 28-29 0.09681287143534689 1102 30-31 -0.023819019496635008 1102 32-33 -0.13243800689070895 1102 34-35 -0.024563976056299452 1102 36-37 -0.2458047246034596 1102 38-39 -0.31999250005765845 1102 40-41 0.021772327824116644 1102 42-43 0.18051031434995934 1102 44-45 0.3019921359569864 1102 46-47 0.3424482865906171 1102 48-49 0.08193847690169065 1102 50-51 0.22255862685314298 1102 52-53 -0.05073885573557391 1102 54-55 0.10004504302668238 1102 56-57 0.20269565183337335 1102 58-59 0.13458108039818484 1102 60-61 0.26414350390996333 1102 62-63 -0.17413377870688151 1102 64-65 0.08694670265181514 1102 66-67 -0.11792769050875762 1102 68-69 -0.10229999154464764 1102 70-71 -0.13259358081046813 1102 72-73 -0.3412980947984927 1102 74-75 -0.2785884263488576 1102 76-77 -0.46341195008124814 1102 78-79 -0.2861519013233895 1102 80-81 -0.2582721812666442 1102 82-83 -0.36717534993156065 1102 84-85 -0.3850547276957492 1102 86-87 -0.47022129465718976 1102 88-89 -0.411439750672681 1102 90-91 -0.44678214247284487 1102 92-93 -0.3981970373425021 1102 94-95 -0.5148187584844557 1102 96-97 -0.38070122624673175 1102 98-99 -0.6524627408316412 1102 100-101 -0.782592994344494 1103 1 0.04770787478967975 1103 2 0.0238189583248527 1103 3 -0.014208928695339296 1103 4 0.020509386665821694 1103 5 0.06342516793201725 1103 6 0.009897222308445919 1103 7 -0.03543728493704634 1103 8 -0.16277007585072312 1103 9 -0.009865442420078807 1103 10-11 0.12606556807794078 1103 12-13 -0.013374847458386796 1103 14-15 0.044357344730094894 1103 16-17 -0.012065227111463628 1103 18-19 -0.07745351936789291 1103 20-21 -0.1295579745930766 1103 22-23 0.0568480462257952 1103 24-25 0.02916592587839517 1103 26-27 0.019738314804875756 1103 28-29 0.08408229624735242 1103 30-31 0.07908064887056554 1103 32-33 0.1612728341684786 1103 34-35 0.14504614354619605 1103 36-37 0.017797869702853575 1103 38-39 0.01821758677701979 1103 40-41 0.28185746762400754 1103 42-43 0.2987950326880693 1103 44-45 0.18927935707753107 1103 46-47 0.17904851370540342 1103 48-49 0.1620487010221794 1103 50-51 0.47291822527802907 1103 52-53 0.4896243019510109 1103 54-55 0.18905568126444905 1103 56-57 0.42131567522281443 1103 58-59 0.26231733345603914 1103 60-61 0.2676668905192301 1103 62-63 0.21589689873926687 1103 64-65 0.15904604560081026 1103 66-67 0.10896168424761044 1103 68-69 0.17186428815682575 1103 70-71 0.1410300300887819 1103 72-73 0.22454852927002733 1103 74-75 0.41548709779479864 1103 76-77 0.3131198036178411 1103 78-79 0.1837045417877441 1103 80-81 0.2846005384047743 1103 82-83 0.16103038669437453 1103 84-85 0.08140007815499573 1103 86-87 0.07364971317883473 1103 88-89 0.15474585070130686 1103 90-91 0.3478608861763881 1103 92-93 0.13708089579987615 1103 94-95 -0.20532428663934255 1103 96-97 -0.1372932049137603 1103 98-99 -0.21812010257127312 1103 100-101 -0.1467910587840784 1104 1 -0.04129205378128731 1104 2 -0.18067977218638376 1104 3 -0.038529865844282085 1104 4 -0.18351011995435584 1104 5 -0.010373363227273558 1104 6 -0.2083230258749822 1104 7 -0.16575891040214685 1104 8 -0.1653116507960135 1104 9 -0.09334780269514908 1104 10-11 -0.2967774373961163 1104 12-13 -0.14472602506343435 1104 14-15 -0.20872644152826325 1104 16-17 -0.23008060383468631 1104 18-19 -0.2837318068892358 1104 20-21 -0.32059947486255425 1104 22-23 -0.2891214081213107 1104 24-25 -0.3057625476831234 1104 26-27 -0.008259269595022545 1104 28-29 -0.2161940042481021 1104 30-31 -0.15058025801844366 1104 32-33 -0.11056464529566767 1104 34-35 0.09583088890168057 1104 36-37 0.14283017510743434 1104 38-39 0.16396723450201023 1104 40-41 0.280390899659281 1104 42-43 0.19957198578516255 1104 44-45 0.29178197073086665 1104 46-47 0.2916476214918191 1104 48-49 0.2732562413308841 1104 50-51 0.3414225662153072 1104 52-53 0.3121699028964784 1104 54-55 0.21305940856138506 1104 56-57 0.09530316864808697 1104 58-59 0.21242610039608678 1104 60-61 0.24640603817602624 1104 62-63 0.3217917422122767 1104 64-65 0.36392140193428446 1104 66-67 0.48306995767795513 1104 68-69 0.27940979169905944 1104 70-71 0.27485882118482863 1104 72-73 0.37926154688702596 1104 74-75 0.39786198334600087 1104 76-77 0.2423612272634479 1104 78-79 0.36973886668596023 1104 80-81 0.4131974773459355 1104 82-83 0.27954933811613714 1104 84-85 0.40781193797226933 1104 86-87 0.40669797972601884 1104 88-89 0.3684170433713909 1104 90-91 0.32747374080531344 1104 92-93 -0.10962655035237034 1104 94-95 0.16670377847796303 1104 96-97 0.14247387149582735 1104 98-99 0.1749710863325511 1104 100-101 0.06255283305937809 1105 1 0.09552479532948865 1105 2 0.030690942744271865 1105 3 0.16224725475144197 1105 4 0.06994482023982584 1105 5 0.028466434274982078 1105 6 0.009805391781682715 1105 7 0.06687914010054641 1105 8 0.08029193653874245 1105 9 -0.11435136365244603 1105 10-11 0.1471263254502162 1105 12-13 0.08689547865477465 1105 14-15 0.11831248551776952 1105 16-17 0.015216091881001148 1105 18-19 0.0666993631540791 1105 20-21 -0.048108123626370514 1105 22-23 0.0989006880752612 1105 24-25 -0.07248281470419471 1105 26-27 0.2051125815955217 1105 28-29 0.19333383919151714 1105 30-31 0.2568607224115098 1105 32-33 0.2878258222955665 1105 34-35 0.3578184306237304 1105 36-37 0.21982598514912866 1105 38-39 0.4173120995204229 1105 40-41 0.5265418193196183 1105 42-43 0.3785278441179045 1105 44-45 0.3769857366207461 1105 46-47 0.5445856648928924 1105 48-49 0.5222128098260512 1105 50-51 0.6031549567234791 1105 52-53 0.6987549347016113 1105 54-55 0.6373302239292968 1105 56-57 0.5816259476151089 1105 58-59 0.43330386115250974 1105 60-61 0.4970872868444758 1105 62-63 0.40427000616950437 1105 64-65 0.38416762539197435 1105 66-67 0.23908554067057963 1105 68-69 0.2482194580241739 1105 70-71 0.28349743981745945 1105 72-73 0.4875090689274231 1105 74-75 0.4888769069020924 1105 76-77 0.4650275828113415 1105 78-79 0.4680319405732867 1105 80-81 0.4961972034261457 1105 82-83 0.3658995090623769 1105 84-85 0.25464410630105405 1105 86-87 0.6410150571032851 1105 88-89 0.38399597792485096 1105 90-91 0.3665818782521768 1105 92-93 0.4626421142379158 1105 94-95 0.5715983193579568 1105 96-97 0.5868316310348298 1105 98-99 0.6118183709757972 1105 100-101 0.7759794682083587 1106 1 0.05743784587503953 1106 2 -0.009309328168502162 1106 3 -0.0724478667868027 1106 4 -0.07974464599428188 1106 5 -0.1887954503297209 1106 6 -0.05649373655646883 1106 7 0.03920540035290543 1106 8 -0.2789011497672931 1106 9 -0.12356239792968182 1106 10-11 -0.3968038738738642 1106 12-13 -0.38571693321391365 1106 14-15 -0.19022837828760686 1106 16-17 -0.15268379230378315 1106 18-19 -0.09968679591235485 1106 20-21 -0.12685400067260844 1106 22-23 -0.23882256098162458 1106 24-25 -0.43169960587785283 1106 26-27 -0.39195715812503096 1106 28-29 -0.061273371151933986 1106 30-31 -0.1425205805434686 1106 32-33 -0.11835708446303528 1106 34-35 0.08032417058809926 1106 36-37 -0.1207604732759151 1106 38-39 -0.08942008288514813 1106 40-41 -0.31856888241219394 1106 42-43 -0.015618069501563525 1106 44-45 0.35127611447634166 1106 46-47 0.20708422566878681 1106 48-49 -0.06714099548248242 1106 50-51 0.3256302190009066 1106 52-53 0.1862167529326797 1106 54-55 0.0282488891420698 1106 56-57 0.10008357347102503 1106 58-59 0.3426378221004356 1106 60-61 0.3813855211704791 1106 62-63 0.15294253358248255 1106 64-65 0.14146533821088525 1106 66-67 0.1676373764432597 1106 68-69 0.2850551291089758 1106 70-71 0.3834524005689737 1106 72-73 0.2890028142287804 1106 74-75 0.230024832048894 1106 76-77 0.13318144772068408 1106 78-79 0.1959000602354557 1106 80-81 0.28709317969418535 1106 82-83 0.42079828927761653 1106 84-85 0.6040809463744701 1106 86-87 0.749249789475332 1106 88-89 0.5461609773491638 1106 90-91 0.45075278357493787 1106 92-93 0.3329493970505304 1106 94-95 0.3220893487587624 1106 96-97 0.5059133721912197 1106 98-99 0.6444616669005931 1106 100-101 0.784340513660748 1107 1 -0.11766803540057325 1107 2 0.05680179566108379 1107 3 -0.057171201875284794 1107 4 -0.279041823256712 1107 5 -0.247477714929893 1107 6 -9.707402880678728E-4 1107 7 -0.20123094496886296 1107 8 -0.08592479849328072 1107 9 -0.25031086980532535 1107 10-11 -0.09835145749145369 1107 12-13 -0.18317463793679423 1107 14-15 0.03153966731574798 1107 16-17 -0.06925621509630986 1107 18-19 0.05477853226236107 1107 20-21 0.0571516310665956 1107 22-23 -0.17348408429840134 1107 24-25 -0.09986768811040037 1107 26-27 -0.1692928418319113 1107 28-29 -0.19285804183255095 1107 30-31 -0.17379962635286716 1107 32-33 -0.016654971416173225 1107 34-35 0.13713605676286988 1107 36-37 -0.22075474996372435 1107 38-39 -0.06394333097927785 1107 40-41 -0.20618363482170565 1107 42-43 0.03951917552488027 1107 44-45 0.18081183939106182 1107 46-47 0.00427064539361055 1107 48-49 0.10948270818730776 1107 50-51 -0.07799034829380247 1107 52-53 -0.23312689762477135 1107 54-55 0.16113046236612405 1107 56-57 -0.05119043582363503 1107 58-59 -0.14010639163093686 1107 60-61 0.07799617568846173 1107 62-63 -0.04924491633853023 1107 64-65 0.07141428626610491 1107 66-67 0.15762501408891438 1107 68-69 0.1803356858727838 1107 70-71 0.22401351409659398 1107 72-73 0.268380575726912 1107 74-75 0.24322736862085748 1107 76-77 0.17677362738690405 1107 78-79 0.18548935535232758 1107 80-81 0.6001698033978577 1107 82-83 0.5093768474608105 1107 84-85 0.26522423521859295 1107 86-87 0.2528863820441849 1107 88-89 0.03894960398316627 1107 90-91 0.33322800163313104 1107 92-93 0.3174815735116958 1107 94-95 0.3634460026815205 1107 96-97 0.6284105677682454 1107 98-99 0.4589702633155106 1107 100-101 0.3359796702734634 1108 1 -0.20810313506027 1108 2 -0.1954344707463065 1108 3 -0.13392436181694478 1108 4 -0.026079377948008187 1108 5 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199.0 8 428.0 9 751.0 10 1397.0 11 2285.0 12 3523.0 13 4707.0 14 5286.0 15 6556.0 16 8124.0 17 9949.0 18 8712.0 19 6535.0 20 5927.0 21 6008.0 22 6391.0 23 7077.0 24 7943.0 25 9233.0 26 11354.0 27 14065.0 28 17634.0 29 21468.0 30 26542.0 31 32073.0 32 39739.0 33 49433.0 34 63510.0 35 84087.0 36 117628.0 37 156812.0 38 146602.0 39 34754.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.25199013960168 20.441884649926394 13.700453844584146 19.60567136588778 2 15.371610751358208 24.06665117856796 38.518247052625725 22.04349101744811 3 21.777952343545433 27.787597102208256 29.90387972418609 20.530570830060224 4 12.3443473139448 17.79684032000349 40.65505826921084 29.203754096840868 5 12.00165782312554 40.687124058612774 33.29039716861261 14.020820949649076 6 26.60136293633377 40.081409705467884 18.309657913450923 15.007569444747414 7 27.263446636091466 33.54790508962606 22.648699644985903 16.539948629296568 8 24.104530110757853 34.11429163508259 20.00850725024949 21.772671003910062 9 24.45245483249988 20.806552764038326 23.103837533333696 31.637154870128096 10-11 24.21475240780904 26.814548123733108 28.539728780986856 20.430970687470996 12-13 27.890201938126115 24.483648083414682 27.70980460591254 19.91634537254667 14-15 22.667226565375902 27.654942424052376 28.381029250162925 21.296801760408798 16-17 19.772321988515213 29.818293860055732 29.424997136983087 20.984387014445964 18-19 19.16983416315379 31.590474061066786 29.995310105631688 19.24438167014773 20-21 21.60590789100508 28.55531557475402 28.91397779086764 20.92479874337326 22-23 20.136443205924536 29.121735479050898 28.999525557197625 21.742295757826945 24-25 21.864778347957444 29.302787215129815 29.141640263288487 19.69079417362425 26-27 22.221555641968596 27.11670287106417 31.547892064174228 19.11384942279301 28-29 20.340709834880897 30.17488171691141 30.4711416533726 19.013266794835086 30-31 21.558277798593647 29.164341924924344 29.29365483216319 19.98372544431882 32-33 21.27302881120315 29.437939122842323 29.46242610956318 19.82660595639135 34-35 22.242782901586335 27.53773048276148 30.50615811435119 19.713328501300996 36-37 22.683871798242432 27.651338084980924 31.032127894120475 18.632662222656162 38-39 21.75060251543034 28.779921933415974 30.759401231373012 18.710074319780666 40-41 20.279355293663375 29.360883823385848 30.481642732601106 19.87811815034967 42-43 20.00588963478811 30.193812611453158 28.55774841443397 21.242549339324764 44-45 21.2295157956733 28.84972160568895 28.377514683186728 21.54324791545102 46-47 22.05831829113337 29.427233016856352 28.087341102561446 20.427107589448827 48-49 22.483571736297055 29.25834228594177 28.447317762156914 19.81076821560426 50-51 21.121757292513074 29.478058383731522 30.041990914693002 19.3581934090624 52-53 21.10561533050122 30.08605410829293 28.672432691835763 20.13589786937008 54-55 20.158583870035393 30.411401896680534 28.387385274822357 21.042628958461716 56-57 21.578156042137824 28.987831686478195 28.577076801674433 20.856935469709555 58-59 22.876700226751435 27.745584672955502 28.27047225157957 21.107242848713494 60-61 21.259309595591606 29.04053900758729 29.81622956470142 19.883921832119686 62-63 20.86012872697087 28.393890786602405 29.69283220606787 21.053148280358855 64-65 20.13328597283537 30.3598797654455 29.00328522532521 20.503549036393924 66-67 20.759710760932652 27.9532670311975 28.962799963343265 22.32422224452658 68-69 20.127218123347262 29.72496606589624 27.971626781029187 22.17618902972731 70-71 21.532921748984446 29.313578302549942 27.38815537972664 21.765344568738968 72-73 20.769469878335414 28.02364579300115 29.761251656459788 21.44563267220365 74-75 19.92387101699814 30.511798356355623 28.670742148516958 20.893588478129278 76-77 20.830656639745218 28.769557132184126 27.810582801175748 22.58920342689491 78-79 20.658941424823407 29.68737474294184 27.634835899961224 22.018847932273523 80-81 21.287103335823705 29.049151183652995 27.50268578253069 22.161059697992616 82-83 20.59882446995898 27.460591167928033 28.891175671828833 23.049408690284153 84-85 19.663547875006206 28.145036082330865 28.600012106517724 23.59140393614521 86-87 20.487962628687477 28.733395647939204 27.603825258792845 23.174816464580474 88-89 20.16365549999182 28.892530525213633 26.978617353699835 23.96519662109471 90-91 19.799316147960713 27.63694764223741 27.844502734862818 24.71923347493906 92-93 19.924034617964477 29.42412459849596 26.945733559466223 23.706107224073335 94-95 19.66656868738483 29.728404702787394 27.105813807007717 23.499212802820058 96-97 18.59704805298562 30.025712969098084 28.05565780755356 23.321581170362734 98-99 19.501054149356552 30.341668729883637 26.61135427380398 23.545922846955836 100-101 18.145066513677662 28.851060952192935 27.000359386000746 26.00351314812866 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 358.0 1 237.0 2 289.5 3 410.0 4 300.5 5 263.0 6 325.5 7 466.0 8 657.0 9 785.5 10 787.0 11 774.0 12 837.0 13 924.5 14 1036.0 15 1157.5 16 1262.0 17 1373.0 18 1474.0 19 1563.5 20 1779.5 21 2080.5 22 2384.0 23 2864.5 24 3590.0 25 4369.5 26 5451.5 27 7144.0 28 9473.5 29 12377.0 30 15202.0 31 17921.5 32 20605.5 33 22579.5 34 23998.0 35 25613.5 36 27073.0 37 28302.5 38 29679.5 39 30238.0 40 30038.0 41 30206.0 42 30749.5 43 31556.0 44 31722.0 45 31794.5 46 34326.0 47 42342.5 48 65187.5 49 65083.0 50 42696.0 51 32537.0 52 24737.0 53 20229.0 54 17857.0 55 16215.5 56 14450.5 57 12024.5 58 9491.5 59 7523.0 60 5706.0 61 4108.5 62 2993.5 63 2080.5 64 1322.5 65 853.0 66 529.5 67 301.0 68 172.5 69 97.5 70 47.5 71 23.0 72 14.0 73 8.5 74 3.5 75 2.0 76 1.5 77 0.5 78 0.5 79 0.5 80 1.5 81 1.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05147977074051251 2 0.0034901539485093225 3 0.03326552982172948 4 0.0 5 0.0 6 0.002290413528709243 7 0.0 8 0.0 9 0.0 10-11 4.908028990091235E-4 12-13 0.0 14-15 0.0035446876039547806 16-17 0.0 18-19 0.0 20-21 0.013633413861364541 22-23 0.0 24-25 0.0 26-27 0.008670851215827849 28-29 0.011179399366318924 30-31 0.010524995500973425 32-33 0.005616966510882191 34-35 0.007252976174245937 36-37 0.021867995833628726 38-39 0.009107120459391512 40-41 0.008234581972264184 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0017996106297001194 58-59 2.1813462178183265E-4 60-61 0.0075256444514732275 62-63 0.012924476340573586 64-65 0.05338844868110355 66-67 0.027594029655401833 68-69 0.0015814760079182865 70-71 1.636009663363745E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.4533655445458164E-5 80-81 0.0 82-83 2.1813462178183265E-4 84-85 3.817355881182072E-4 86-87 0.001308807730690996 88-89 0.0 90-91 0.0 92-93 0.0 94-95 4.908028990091235E-4 96-97 0.001363341386136454 98-99 0.001308807730690996 100-101 0.0025630818059365337 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 916865.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.46386568989865 #Duplication Level Percentage of deduplicated Percentage of total 1 86.58323197549483 55.814898370656366 2 8.91136236368769 11.48921733053562 3 1.9178625012338475 3.708984920736954 4 0.7747987439754703 1.9978608867334757 5 0.4136134201116521 1.3331559980808583 6 0.26878221227109184 1.0396044259006496 7 0.18974859523040324 0.8562349570445761 8 0.13263810417629576 0.6840291946386811 9 0.09502668732342581 0.5513208847715985 >10 0.6318547463079996 7.533509658749132 >50 0.04763688752626675 2.1224444531706292 >100 0.02584290765027063 3.0069559072314473 >500 0.0028714341152127044 1.3182653048244743 >1k 0.004391605117384136 5.879112651250737 >5k 1.6890788913015908E-4 0.6116045442470532 >10k+ 1.6890788913015908E-4 2.052800511427754 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18853 2.056246012226446 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5617 0.612631085274277 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4655 0.5077083321972156 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4623 0.5042181782487062 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3761 0.41020215626073636 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3642 0.3972231462647173 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3043 0.3318918270410584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2748 0.2997169703282381 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2618 0.28553821991241896 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2221 0.24223849748872517 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2159 0.23547632421348835 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2142 0.2336221799283428 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1882 0.20526467909670454 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1775 0.19359447683137648 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1615 0.17614370708882987 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1614 0.17603463977793896 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1589 0.17330795700566606 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1499 0.16349189902548358 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1479 0.16131055280766526 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1458 0.159020139278956 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1413 0.1541121102888648 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1291 0.140805898360173 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1207 0.13164424424533602 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1204 0.13131704231266328 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1159 0.126409013322572 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1082 0.11801083038397148 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1081 0.11790176307308056 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1034 0.11277559946120748 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 951 0.10372301265726143 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.1813462178183265E-4 0.0 10-11 0.0 0.0 0.0 3.8173558811820715E-4 0.0 12-13 0.0 0.0 0.0 5.998702099000398E-4 0.0 14-15 0.0 0.0 0.0 6.54403865345498E-4 0.0 16-17 0.0 0.0 0.0 8.180048316818725E-4 0.0 18-19 0.0 0.0 0.0 0.0011997404198000796 0.0 20-21 0.0 0.0 0.0 0.002017745251481952 0.0 22-23 0.0 0.0 0.0 0.004308158780191195 0.0 24-25 0.0 0.0 0.0 0.008289115627709641 0.0 26-27 0.0 0.0 0.0 0.012324606130673545 0.0 28-29 0.0 0.0 0.0 0.024212943017783428 0.0 30-31 0.0 0.0 0.0 0.04362692435636653 0.0 32-33 0.0 0.0 0.0 0.07307509829691394 0.0 34-35 0.0 0.0 0.0 0.1005055269859794 0.0 36-37 0.0 1.0906731089091633E-4 0.0 0.12662714794435387 0.0 38-39 0.0 2.1813462178183265E-4 0.0 0.18879551515217616 0.0 40-41 0.0 2.1813462178183265E-4 0.0 0.3156953313737573 0.0 42-43 0.0 2.1813462178183265E-4 0.0 0.5089080726170156 0.0 44-45 0.0 2.1813462178183265E-4 0.0 0.6744722505494265 0.0 46-47 0.0 2.1813462178183265E-4 0.0 1.0614430695903978 0.0 48-49 0.0 2.1813462178183265E-4 0.0 1.3644320592453631 0.0 50-51 0.0 2.1813462178183265E-4 0.0 1.5449384587698298 0.0 52-53 0.0 2.1813462178183265E-4 0.0 1.8035370528921923 0.0 54-55 0.0 2.1813462178183265E-4 0.0 2.018072453414625 0.0 56-57 0.0 2.1813462178183265E-4 0.0 2.6355025003681023 0.0 58-59 0.0 2.1813462178183265E-4 0.0 3.4839371117885403 0.0 60-61 0.0 2.1813462178183265E-4 0.0 4.145539419652838 0.0 62-63 0.0 2.1813462178183265E-4 0.0 4.714216378638076 0.0 64-65 0.0 2.1813462178183265E-4 0.0 5.5838645820267985 0.0 66-67 0.0 2.1813462178183265E-4 0.0 6.764027419521958 0.0 68-69 0.0 2.1813462178183265E-4 0.0 7.8042568971440724 0.0 70-71 0.0 2.1813462178183265E-4 0.0 8.337214311812534 0.0 72-73 0.0 2.1813462178183265E-4 0.0 8.745507790132681 0.0 74-75 0.0 2.1813462178183265E-4 0.0 9.3168023645793 0.0 76-77 0.0 2.1813462178183265E-4 0.0 9.893713905536803 0.0 78-79 0.0 2.1813462178183265E-4 0.0 10.434796834866638 0.0 80-81 0.0 2.1813462178183265E-4 0.0 11.422292267672994 0.0 82-83 0.0 2.1813462178183265E-4 0.0 12.527416795275204 0.0 84-85 0.0 2.1813462178183265E-4 0.0 14.349822492951525 0.0 86-87 0.0 2.1813462178183265E-4 0.0 15.373201071040993 0.0 88-89 0.0 2.1813462178183265E-4 0.0 16.595300289573707 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 240 0.0 53.451923 2 ATAACGC 265 0.0 50.196747 3 TACCTGG 1080 0.0 48.83262 2 TAACGCA 270 0.0 45.733124 4 TCACGCA 150 0.0 41.159817 4 ACCTGGG 1250 0.0 41.0466 3 GTACCCG 105 1.020453E-9 40.736393 1 GTATAAC 380 0.0 33.768322 1 GTACCTG 1605 0.0 33.46052 1 TACACGG 100 1.2960772E-6 33.258972 2 GTACACG 105 1.8930805E-6 31.68386 1 TAGCGTA 60 0.00395376 31.661394 9 CCTGGGG 735 0.0 31.015242 4 TACCCGG 125 2.1480264E-7 30.408205 2 CGGTAGA 65 0.00584822 29.2259 9 CACGCAG 215 1.8189894E-12 28.71615 5 ACACGGG 120 5.373393E-6 27.71279 3 TTGTACG 105 6.9121656E-5 27.138338 4 TATCACG 195 5.329639E-10 26.802105 2 ACGGTAG 95 0.0012279467 24.994474 8 >>END_MODULE