FastQCFastQC Report
Wed 25 May 2016
SRR1003810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905798
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60960.31986600888446726No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58810.30858464538214436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52100.2733762969632668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38420.20159534221360292No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36440.19120599349983577No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35970.18873983496676983No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31340.1644455498431628No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25700.13485164744637154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.12388511269295066No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21400.11228892044172571No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20610.10814367524784893No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19160.10053531381604977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3400.069.396182
TACCTGG25350.063.1098862
TAACGCA4150.058.156424
ACCTGGG30050.051.631623
GTACCTG38200.043.336521
GTACACG2950.043.0166131
TACACGG3200.039.564282
ATAACGC6600.039.1800583
GTATAAC7850.036.004931
TATCACG3150.032.8845982
CCTGGGG22450.028.156124
ACACGGG4800.026.3377023
GTACCCG2656.91216E-1126.1198641
GTACATG73200.023.8763031
TCACGCA4150.023.5395034
AGTACTT68000.023.15464412-13
TACATGG71500.023.019222
TAACGCC2053.063953E-622.4250074
TCTCGGG9000.021.393665114-115
CACGCAG4600.021.2378525
Produced by FastQC (version 0.11.5)