FastQCFastQC Report
Wed 25 May 2016
SRR1003810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905798
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103490.5430271203978596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75390.3955823229954067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64340.33760136173928196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38820.20369420053961648No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32910.17268356877276606No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29880.1567847169532133No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29630.15547293049945482No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28240.1481793978165577No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23980.1258265566445132No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22620.1186904383360671No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22440.117745952089361No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC21040.11039994794831351No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20280.10641211712888775No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20130.10562504525663266No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19930.10457561609362588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG26350.058.4374662
TATAACG4650.054.1688842
ACCTGGG30350.049.6343083
TAACGCA5450.046.1847534
GTACCTG35050.044.2197461
ATAACGC5850.043.05283
CCTGGGG22150.036.664154
GTATAAC7800.036.5715451
TCACGCA3600.034.299684
GTACACG3100.033.74021
TATCACG3750.030.416342
CACGCAG4000.029.6824175
GTACCGG2350.028.3234961
GTACCCG2104.1836756E-1127.1674351
TACACGG3350.026.954782
CTGGGGG14450.026.6217375
TACCGGG2405.8971636E-921.7825362
GTACGAC1100.002870841921.6002463
GGTCGCG1553.914113E-521.4473787
TACCGAC1353.802277E-421.1069437
Produced by FastQC (version 0.11.5)